Rw4G007850

No apical meristem (NAM) protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0006410
Physical Location & Seq
Forward (+)
16870056 .. 16871685
1630 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G007850.1

Sequence Viewer

Length: 519 bp
ATGGGATCCCAGAAAGGAGTAGTAGTGAGCTCTGATTTGCTTCCAGTTGGGTTTAGGTTTATGCCAACAGATGAAGAGCTGGTCACTCACTATCTAATGAACAAAGCTTGTTCCAGAGCTGTACCAGTTGCTGATGCGATTCAGGAGATCGATGCGACTCGGTTTTACAGCAACCATCCAAAGAATCTAGTGACGTTCTCTGATGGAGAGAGGGAGTGGTTCTTCTTTATTCATGAAGACAATGAAAGTTGCAGCAGCCATGCCCAGAGAAAAAGAACTGTTCGAGAAGTCGGAAATGGGTTAGGGTTTTGGAGACCAAACGGATCAGAAAACCCGATCTACAATGAAGATGGAAACGTTTTTGCCACCAAGATCTACCTCACTTACTTCTCCGGAAGTTGTCACTCCAATAAACCAAAGAGAACCCACTGGAAAATGGTCGAGTACCATTTACAGATAAGAAGAGAATGGGTAGTGCTGGGAAGATTACAAAGAGGAATGGATTACACTGGTTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAM

Protein Analysis

172

Amino Acids

19.92

Weight (kDa)

7.78

Isoelectric Point (pI)

50.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 15 - 151 2.6e-27 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 392
AclI AACGTT 1 cut(s) 357
AclWI GGATC 2 cut(s) 13, 331
AfaI GTAC 2 cut(s) 123, 446
AluBI AGCT 4 cut(s) 30, 79, 107, 119
AluI AGCT 4 cut(s) 30, 79, 107, 119
Alw21I GWGCWC 1 cut(s) 32
Alw26I GTCTC 1 cut(s) 307
AlwI GGATC 2 cut(s) 13, 331
AlwNI CAGNNNCTG 1 cut(s) 131
Aor13HI TCCGGA 1 cut(s) 392
ApeKI GCWGC 2 cut(s) 252, 255
BamHI GGATCC 1 cut(s) 5
BanII GRGCYC 1 cut(s) 32
BbsI GAAGAC 1 cut(s) 243
Bbv12I GWGCWC 1 cut(s) 32
BbvI GCAGC 2 cut(s) 264, 267
BccI CCATC 3 cut(s) 183, 197, 344
BcoDI GTCTC 1 cut(s) 307
BfaI CTAG 1 cut(s) 188
BglII AGATCT 1 cut(s) 372
BisI GCNGC 2 cut(s) 253, 256
BlsI GCNGC 2 cut(s) 254, 257
BmiI GGNNCC 1 cut(s) 7
BmsI GCATC 2 cut(s) 124, 142
BpiI GAAGAC 1 cut(s) 243
Bsa29I ATCGAT 1 cut(s) 150
BsaI GGTCTC 1 cut(s) 307
BsaWI WCCGGW 1 cut(s) 392
BsaXI ACNNNNNCTCC 2 cut(s) 9, 39
Bse1I ACTGG 4 cut(s) 44, 125, 434, 514
BseAI TCCGGA 1 cut(s) 392
BseCI ATCGAT 1 cut(s) 150
BseGI GGATG 1 cut(s) 175
BseNI ACTGG 4 cut(s) 44, 125, 434, 514
BseXI GCAGC 2 cut(s) 264, 267
BseYI CCCAGC 1 cut(s) 478
BshVI ATCGAT 1 cut(s) 150
BsiHKAI GWGCWC 1 cut(s) 32
BsiSI CCGG 1 cut(s) 393
BsmAI GTCTC 1 cut(s) 307
Bso31I GGTCTC 1 cut(s) 307
Bsp1286I GDGCHC 1 cut(s) 32
Bsp13I TCCGGA 1 cut(s) 392
Bsp143I GATC 5 cut(s) 5, 147, 323, 336, 372
BspDI ATCGAT 1 cut(s) 150
BspEI TCCGGA 1 cut(s) 392
BspHI TCATGA 1 cut(s) 232
BspLI GGNNCC 1 cut(s) 7
BspPI GGATC 2 cut(s) 13, 331
BspQI GCTCTTC 1 cut(s) 69
BspTNI GGTCTC 1 cut(s) 307
BsrI ACTGG 4 cut(s) 44, 125, 434, 514
BssMI GATC 5 cut(s) 5, 147, 323, 336, 372
Bst4CI ACNGT 1 cut(s) 280
Bst6I CTCTTC 2 cut(s) 69, 457
BstF5I GGATG 1 cut(s) 175
BstKTI GATC 5 cut(s) 8, 150, 326, 339, 375
BstMAI GTCTC 1 cut(s) 307
BstMBI GATC 5 cut(s) 5, 147, 323, 336, 372
BstV1I GCAGC 2 cut(s) 264, 267
BstV2I GAAGAC 1 cut(s) 243
BstX2I RGATCY 2 cut(s) 5, 372
BstYI RGATCY 2 cut(s) 5, 372
Bsu15I ATCGAT 1 cut(s) 150
BsuTUI ATCGAT 1 cut(s) 150
BtsCI GGATG 1 cut(s) 175
BtsIMutI CAGTG 2 cut(s) 427, 507
CaiI CAGNNNCTG 1 cut(s) 131
CciI TCATGA 1 cut(s) 232
ClaI ATCGAT 1 cut(s) 150
Csp6I GTAC 2 cut(s) 122, 445
CviAII CATG 2 cut(s) 233, 260
CviJI RGCY 5 cut(s) 30, 79, 107, 119, 258
CviKI_1 RGCY 5 cut(s) 30, 79, 107, 119, 258
CviQI GTAC 2 cut(s) 122, 445
DpnI GATC 5 cut(s) 7, 149, 325, 338, 374
DpnII GATC 5 cut(s) 5, 147, 323, 336, 372
Eam1104I CTCTTC 2 cut(s) 69, 457
EarI CTCTTC 2 cut(s) 69, 457
Ecl136II GAGCTC 1 cut(s) 30
Eco24I GRGCYC 1 cut(s) 32
Eco31I GGTCTC 1 cut(s) 307
Eco53kI GAGCTC 1 cut(s) 30
EcoICRI GAGCTC 1 cut(s) 30
EcoT38I GRGCYC 1 cut(s) 32
FaeI CATG 2 cut(s) 236, 263
FaiI YATR 3 cut(s) 62, 234, 261
FatI CATG 2 cut(s) 232, 259
Fnu4HI GCNGC 2 cut(s) 253, 256
FokI GGATG 1 cut(s) 162
FriOI GRGCYC 1 cut(s) 32
Fsp4HI GCNGC 2 cut(s) 253, 256
FspBI CTAG 1 cut(s) 188
GluI GCNGC 2 cut(s) 253, 256
GsaI CCCAGC 1 cut(s) 482
HapII CCGG 1 cut(s) 393
Hin1II CATG 2 cut(s) 236, 263
HindIII AAGCTT 1 cut(s) 105
HinfI GANTC 3 cut(s) 139, 157, 184
HpaII CCGG 1 cut(s) 393
Hpy188I TCNGA 4 cut(s) 34, 202, 293, 328
Hpy188III TCNNGA 5 cut(s) 114, 143, 233, 284, 393
HpyCH4III ACNGT 1 cut(s) 280
HpyCH4IV ACGT 2 cut(s) 194, 357
HpyCH4V TGCA 1 cut(s) 252
HpySE526I ACGT 2 cut(s) 194, 357
Hsp92II CATG 2 cut(s) 236, 263
Kpn2I TCCGGA 1 cut(s) 392
Kzo9I GATC 5 cut(s) 5, 147, 323, 336, 372
LguI GCTCTTC 1 cut(s) 69
Lsp1109I GCAGC 2 cut(s) 264, 267
LweI GCATC 2 cut(s) 124, 142
MaeI CTAG 1 cut(s) 188
MaeII ACGT 2 cut(s) 194, 357
MaeIII GTNAC 3 cut(s) 82, 190, 401
MalI GATC 5 cut(s) 7, 149, 325, 338, 374
MboI GATC 5 cut(s) 5, 147, 323, 336, 372
MboII GAAGA 6 cut(s) 86, 214, 248, 359, 474, 495
MflI RGATCY 2 cut(s) 5, 372
MhlI GDGCHC 1 cut(s) 32
MlyI GAGTC 1 cut(s) 151
MmeI TCCRAC 1 cut(s) 271
MnlI CCTC 3 cut(s) 204, 389, 488
MroI TCCGGA 1 cut(s) 392
MspI CCGG 1 cut(s) 393
NdeII GATC 5 cut(s) 5, 147, 323, 336, 372
NlaIII CATG 2 cut(s) 236, 263
NlaIV GGNNCC 1 cut(s) 7
NmuCI GTSAC 3 cut(s) 82, 190, 401
PagI TCATGA 1 cut(s) 232
PciSI GCTCTTC 1 cut(s) 69
PfeI GAWTC 2 cut(s) 139, 184
PkrI GCNGC 2 cut(s) 254, 257
PleI GAGTC 1 cut(s) 151
PpsI GAGTC 1 cut(s) 151
Psp124BI GAGCTC 1 cut(s) 32
Psp1406I AACGTT 1 cut(s) 357
PspFI CCCAGC 1 cut(s) 478
PspN4I GGNNCC 1 cut(s) 7
PstNI CAGNNNCTG 1 cut(s) 131
PsuI RGATCY 2 cut(s) 5, 372
RsaI GTAC 2 cut(s) 123, 446
RsaNI GTAC 2 cut(s) 122, 445
SacI GAGCTC 1 cut(s) 32
SapI GCTCTTC 1 cut(s) 69
SatI GCNGC 2 cut(s) 253, 256
Sau3AI GATC 5 cut(s) 5, 147, 323, 336, 372
SchI GAGTC 1 cut(s) 151
SduI GDGCHC 1 cut(s) 32
SetI ASST 8 cut(s) 32, 59, 81, 109, 121, 197, 360, 381
SfaNI GCATC 2 cut(s) 124, 142
SspMI CTAG 1 cut(s) 188
SstI GAGCTC 1 cut(s) 32
TaaI ACNGT 1 cut(s) 280
TaiI ACGT 2 cut(s) 197, 360
TaqI TCGA 3 cut(s) 150, 283, 441
TfiI GAWTC 2 cut(s) 139, 184
TscAI CASTG 2 cut(s) 434, 514
TseFI GTSAC 3 cut(s) 82, 190, 401
TseI GCWGC 2 cut(s) 252, 255
Tsp45I GTSAC 3 cut(s) 82, 190, 401
TspDTI ATGAA 6 cut(s) 87, 113, 221, 249, 258, 360
TspGWI ACGGA 1 cut(s) 336
TspRI CASTG 2 cut(s) 434, 514
XspI CTAG 1 cut(s) 188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.