FvH4_5g14820

HNH nucleases

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0005496
Physical Location & Seq
Forward (+)
8411312 .. 8416277
4966 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g14820.t8

Sequence Viewer

Length: 609 bp
ATGAGGCCAGCAAGCAAAGAACCAACGCGCCGGAGCCGCCCTTCATCTCCGTTAAGGGACCGAAACCCAATCTCGCCCAGGTCTCGTCACTCCGGGCCCACACCACCACGAGCCTTTCTCCTCGATGACGTCACCGACTTCAACAACCCAAGAAGCTTCCCTCACAATGTGAAGAAGCAATGCTGGGAGAAGGCGGAGAAGATCAAAGGGCGGGACCCGGATCGCTGGCGGCGAGACCCGCTCGGAAACACAGTGTTCCGGAAGCTCGTGGGCTGCCCCGGTTGCTTATGTCATGATTACGATCACATTGTGCCGTACTCTAAGGGAGGAGAAAGTACGCTCGAAAATTGTCAGGTTTTACAGGCAAAAGTGAACAGATCAAAAGGAAACCGAACAGAGTTTTCCAGGGCTGAGCTCATACAGAAAAGTTCTTACTGTCGGGTTTCAGGTCGTGATATGGATCTAATTGAACTGTCAGCCTATGGTAATGTCAGTCCTGCAAAAGAAGATTCATACTGTCGGGTTCCAGGTCGTGATATTGATCTGATTGACTCAGCCAAAGGCAACATCCGCAATGCACAAGAGTTTTGGGGGTGTACAATCCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HNH

Protein Analysis

203

Amino Acids

22.97

Weight (kDa)

9.46

Isoelectric Point (pI)

62.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 132
AccBSI CCGCTC 1 cut(s) 241
AccII CGCG 1 cut(s) 28
AccIII TCCGGA 1 cut(s) 258
AciI CCGC 6 cut(s) 37, 194, 211, 229, 239, 571
AclWI GGATC 2 cut(s) 228, 468
AcyI GRCGYC 1 cut(s) 129
AdeI CACNNNGTG 2 cut(s) 169, 310
AfaI GTAC 3 cut(s) 317, 337, 598
AgsI TTSAA 2 cut(s) 142, 470
AjnI CCWGG 3 cut(s) 77, 404, 526
AluBI AGCT 3 cut(s) 156, 265, 415
AluI AGCT 3 cut(s) 156, 265, 415
Alw21I GWGCWC 1 cut(s) 417
Alw26I GTCTC 2 cut(s) 87, 228
AlwI GGATC 2 cut(s) 228, 468
Aor13HI TCCGGA 1 cut(s) 258
AoxI GGCC 2 cut(s) 5, 95
ApaI GGGCCC 1 cut(s) 99
ApeKI GCWGC 1 cut(s) 273
AspLEI GCGC 1 cut(s) 30
AspS9I GGNCC 4 cut(s) 58, 95, 96, 214
AsuC2I CCSGG 3 cut(s) 94, 218, 279
AsuHPI GGTGA 1 cut(s) 124
AvaII GGWCC 2 cut(s) 58, 214
BaeGI GKGCMC 1 cut(s) 99
BanII GRGCYC 2 cut(s) 99, 417
BauI CACGAG 2 cut(s) 108, 266
Bbv12I GWGCWC 1 cut(s) 417
BbvI GCAGC 1 cut(s) 260
BceAI ACGGC 1 cut(s) 298
BciT130I CCWGG 3 cut(s) 79, 406, 528
BcnI CCSGG 3 cut(s) 94, 218, 279
BcoDI GTCTC 2 cut(s) 87, 228
BisI GCNGC 3 cut(s) 37, 230, 274
BlpI GCTNAGC 1 cut(s) 411
BlsI GCNGC 3 cut(s) 38, 231, 275
Bme1390I CCNGG 6 cut(s) 79, 94, 218, 279, 406, 528
Bme18I GGWCC 2 cut(s) 58, 214
BmgT120I GGNCC 4 cut(s) 58, 95, 96, 214
BmiI GGNNCC 6 cut(s) 35, 59, 97, 215, 216, 525
BmrFI CCNGG 6 cut(s) 79, 94, 218, 279, 406, 528
BplI GAGNNNNNCTC 4 cut(s) 102, 134, 225, 257
Bpu1102I GCTNAGC 1 cut(s) 411
BpuMI CCSGG 3 cut(s) 94, 218, 279
BsaBI GATNNNNATC 3 cut(s) 300, 459, 540
BsaHI GRCGYC 1 cut(s) 129
BsaI GGTCTC 2 cut(s) 87, 228
BsaJI CCNNGG 3 cut(s) 77, 277, 405
BsaWI WCCGGW 1 cut(s) 258
Bse3DI GCAATG 2 cut(s) 185, 580
Bse8I GATNNNNATC 3 cut(s) 300, 459, 540
BseAI TCCGGA 1 cut(s) 258
BseBI CCWGG 3 cut(s) 79, 406, 528
BseDI CCNNGG 3 cut(s) 77, 277, 405
BseGI GGATG 1 cut(s) 567
BseJI GATNNNNATC 3 cut(s) 300, 459, 540
BseMI GCAATG 2 cut(s) 185, 580
BseMII CTCAG 2 cut(s) 402, 567
BseRI GAGGAG 2 cut(s) 110, 342
BseSI GKGCMC 1 cut(s) 99
BseXI GCAGC 1 cut(s) 260
BseYI CCCAGC 1 cut(s) 183
Bsh1236I CGCG 1 cut(s) 28
BshFI GGCC 2 cut(s) 7, 97
BsiHKAI GWGCWC 1 cut(s) 417
BsiSI CCGG 5 cut(s) 31, 93, 218, 259, 279
BslFI GGGAC 2 cut(s) 71, 227
BsmAI GTCTC 2 cut(s) 87, 228
BsmFI GGGAC 2 cut(s) 71, 227
BsnI GGCC 2 cut(s) 7, 97
Bso31I GGTCTC 2 cut(s) 87, 228
Bsp120I GGGCCC 1 cut(s) 95
Bsp1286I GDGCHC 2 cut(s) 99, 417
Bsp13I TCCGGA 1 cut(s) 258
Bsp1407I TGTACA 1 cut(s) 596
Bsp143I GATC 6 cut(s) 201, 220, 301, 377, 460, 541
Bsp1720I GCTNAGC 1 cut(s) 411
BspACI CCGC 6 cut(s) 37, 194, 211, 229, 239, 571
BspANI GGCC 2 cut(s) 7, 97
BspCNI CTCAG 2 cut(s) 403, 566
BspEI TCCGGA 1 cut(s) 258
BspFNI CGCG 1 cut(s) 28
BspHI TCATGA 1 cut(s) 292
BspLI GGNNCC 6 cut(s) 35, 59, 97, 215, 216, 525
BspPI GGATC 2 cut(s) 228, 468
BspTNI GGTCTC 2 cut(s) 87, 228
BsrBI CCGCTC 1 cut(s) 241
BsrDI GCAATG 2 cut(s) 185, 580
BsrGI TGTACA 1 cut(s) 596
BssECI CCNNGG 3 cut(s) 77, 277, 405
BssMI GATC 6 cut(s) 201, 220, 301, 377, 460, 541
BssNI GRCGYC 1 cut(s) 129
BssSI CACGAG 2 cut(s) 108, 266
Bst2BI CACGAG 2 cut(s) 108, 266
Bst2UI CCWGG 3 cut(s) 79, 406, 528
Bst4CI ACNGT 4 cut(s) 253, 437, 474, 518
BstACI GRCGYC 1 cut(s) 129
BstAUI TGTACA 1 cut(s) 596
BstC8I GCNNGC 3 cut(s) 9, 13, 227
BstDEI CTNAG 3 cut(s) 321, 411, 553
BstF5I GGATG 1 cut(s) 567
BstFNI CGCG 1 cut(s) 28
BstHHI GCGC 1 cut(s) 30
BstKTI GATC 6 cut(s) 204, 223, 304, 380, 463, 544
BstMAI GTCTC 2 cut(s) 87, 228
BstMBI GATC 6 cut(s) 201, 220, 301, 377, 460, 541
BstMWI GCNNNNNNNGC 4 cut(s) 36, 238, 282, 570
BstNI CCWGG 3 cut(s) 79, 406, 528
BstSCI CCNGG 6 cut(s) 77, 92, 216, 277, 404, 526
BstSLI GKGCMC 1 cut(s) 99
BstUI CGCG 1 cut(s) 28
BstV1I GCAGC 1 cut(s) 260
BstX2I RGATCY 1 cut(s) 460
BstYI RGATCY 1 cut(s) 460
BsuRI GGCC 2 cut(s) 7, 97
BtsCI GGATG 1 cut(s) 567
BtsIMutI CAGTG 1 cut(s) 258
Cac8I GCNNGC 3 cut(s) 9, 13, 227
CciI TCATGA 1 cut(s) 292
CfoI GCGC 1 cut(s) 30
Cfr13I GGNCC 4 cut(s) 58, 95, 96, 214
Csp6I GTAC 3 cut(s) 316, 336, 597
CviAII CATG 1 cut(s) 293
CviQI GTAC 3 cut(s) 316, 336, 597
DdeI CTNAG 3 cut(s) 321, 411, 553
DpnI GATC 6 cut(s) 203, 222, 303, 379, 462, 543
DpnII GATC 6 cut(s) 201, 220, 301, 377, 460, 541
DraIII CACNNNGTG 2 cut(s) 169, 310
EciI GGCGGA 1 cut(s) 209
Ecl136II GAGCTC 1 cut(s) 415
Eco24I GRGCYC 2 cut(s) 99, 417
Eco31I GGTCTC 2 cut(s) 87, 228
Eco47I GGWCC 2 cut(s) 58, 214
Eco53kI GAGCTC 1 cut(s) 415
EcoICRI GAGCTC 1 cut(s) 415
EcoO109I RGGNCCY 1 cut(s) 214
EcoRII CCWGG 3 cut(s) 77, 404, 526
EcoT38I GRGCYC 2 cut(s) 99, 417
FaeI CATG 1 cut(s) 296
FaiI YATR 6 cut(s) 289, 294, 419, 458, 483, 514
FaqI GGGAC 2 cut(s) 71, 227
FatI CATG 1 cut(s) 292
FauI CCCGC 2 cut(s) 204, 246
Fnu4HI GCNGC 3 cut(s) 37, 230, 274
FokI GGATG 1 cut(s) 554
FriOI GRGCYC 2 cut(s) 99, 417
Fsp4HI GCNGC 3 cut(s) 37, 230, 274
GlaI GCGC 1 cut(s) 29
GluI GCNGC 3 cut(s) 37, 230, 274
GsaI CCCAGC 1 cut(s) 187
HaeIII GGCC 2 cut(s) 7, 97
HapII CCGG 5 cut(s) 31, 93, 218, 259, 279
HhaI GCGC 1 cut(s) 30
Hin1I GRCGYC 1 cut(s) 129
Hin1II CATG 1 cut(s) 296
Hin6I GCGC 1 cut(s) 28
HinP1I GCGC 1 cut(s) 28
HindIII AAGCTT 1 cut(s) 154
HinfI GANTC 2 cut(s) 509, 551
HpaII CCGG 5 cut(s) 31, 93, 218, 259, 279
HphI GGTGA 1 cut(s) 124
Hpy166II GTNNAC 2 cut(s) 373, 597
Hpy188I TCNGA 2 cut(s) 245, 546
Hpy188III TCNNGA 4 cut(s) 259, 293, 452, 533
Hpy8I GTNNAC 2 cut(s) 373, 597
HpyAV CCTTC 2 cut(s) 51, 184
HpyCH4III ACNGT 4 cut(s) 253, 437, 474, 518
HpyCH4IV ACGT 1 cut(s) 129
HpyCH4V TGCA 2 cut(s) 500, 578
HpyF10VI GCNNNNNNNGC 4 cut(s) 36, 238, 282, 570
HpyF3I CTNAG 3 cut(s) 321, 411, 553
HpySE526I ACGT 1 cut(s) 129
Hsp92I GRCGYC 1 cut(s) 129
Hsp92II CATG 1 cut(s) 296
HspAI GCGC 1 cut(s) 28
KflI GGGWCCC 1 cut(s) 214
Kpn2I TCCGGA 1 cut(s) 258
Kzo9I GATC 6 cut(s) 201, 220, 301, 377, 460, 541
LmnI GCTCC 1 cut(s) 33
Lsp1109I GCAGC 1 cut(s) 260
MaeII ACGT 1 cut(s) 129
MaeIII GTNAC 2 cut(s) 86, 130
MalI GATC 6 cut(s) 203, 222, 303, 379, 462, 543
MbiI CCGCTC 1 cut(s) 241
MboI GATC 6 cut(s) 201, 220, 301, 377, 460, 541
MboII GAAGA 3 cut(s) 184, 211, 518
MflI RGATCY 1 cut(s) 460
MhlI GDGCHC 2 cut(s) 99, 417
MluCI AATT 2 cut(s) 346, 465
MlyI GAGTC 1 cut(s) 545
MnlI CCTC 3 cut(s) 131, 171, 320
MroI TCCGGA 1 cut(s) 258
MseI TTAA 1 cut(s) 53
MspI CCGG 5 cut(s) 31, 93, 218, 259, 279
MspR9I CCNGG 6 cut(s) 79, 94, 218, 279, 406, 528
MvaI CCWGG 3 cut(s) 79, 406, 528
MvnI CGCG 1 cut(s) 28
MwoI GCNNNNNNNGC 4 cut(s) 36, 238, 282, 570
NciI CCSGG 3 cut(s) 94, 218, 279
NdeII GATC 6 cut(s) 201, 220, 301, 377, 460, 541
NlaIII CATG 1 cut(s) 296
NlaIV GGNNCC 6 cut(s) 35, 59, 97, 215, 216, 525
NmuCI GTSAC 2 cut(s) 86, 130
PagI TCATGA 1 cut(s) 292
PcsI WCGNNNNNNNCGW 1 cut(s) 229
PfeI GAWTC 1 cut(s) 509
PkrI GCNGC 3 cut(s) 38, 231, 275
PleI GAGTC 1 cut(s) 545
PpsI GAGTC 1 cut(s) 545
PpuMI RGGWCCY 1 cut(s) 214
Psp124BI GAGCTC 1 cut(s) 417
Psp5II RGGWCCY 1 cut(s) 214
Psp6I CCWGG 3 cut(s) 77, 404, 526
PspFI CCCAGC 1 cut(s) 183
PspGI CCWGG 3 cut(s) 77, 404, 526
PspN4I GGNNCC 6 cut(s) 35, 59, 97, 215, 216, 525
PspOMI GGGCCC 1 cut(s) 95
PspPI GGNCC 4 cut(s) 58, 95, 96, 214
PspPPI RGGWCCY 1 cut(s) 214
PsuI RGATCY 1 cut(s) 460
RsaI GTAC 3 cut(s) 317, 337, 598
RsaNI GTAC 3 cut(s) 316, 336, 597
SacI GAGCTC 1 cut(s) 417
SaqAI TTAA 1 cut(s) 53
SatI GCNGC 3 cut(s) 37, 230, 274
Sau3AI GATC 6 cut(s) 201, 220, 301, 377, 460, 541
Sau96I GGNCC 4 cut(s) 58, 95, 96, 214
SchI GAGTC 1 cut(s) 545
ScrFI CCNGG 6 cut(s) 79, 94, 218, 279, 406, 528
SduI GDGCHC 2 cut(s) 99, 417
SetI ASST 8 cut(s) 83, 132, 158, 267, 357, 417, 451, 532
SinI GGWCC 2 cut(s) 58, 214
Sse9I AATT 2 cut(s) 346, 465
SsiI CCGC 6 cut(s) 37, 194, 211, 229, 239, 571
SstI GAGCTC 1 cut(s) 417
StyD4I CCNGG 6 cut(s) 77, 92, 216, 277, 404, 526
TaaI ACNGT 4 cut(s) 253, 437, 474, 518
TaiI ACGT 1 cut(s) 132
TaqI TCGA 2 cut(s) 123, 342
TaqII GACCGA 1 cut(s) 75
TasI AATT 2 cut(s) 346, 465
TatI WGTACW 1 cut(s) 596
TauI GCSGC 2 cut(s) 39, 232
TfiI GAWTC 1 cut(s) 509
Tru1I TTAA 1 cut(s) 53
Tru9I TTAA 1 cut(s) 53
TscAI CASTG 1 cut(s) 258
TseFI GTSAC 2 cut(s) 86, 130
TseI GCWGC 1 cut(s) 273
Tsp45I GTSAC 2 cut(s) 86, 130
TspDTI ATGAA 2 cut(s) 33, 501
TspGWI ACGGA 1 cut(s) 39
TspRI CASTG 1 cut(s) 258
VpaK11BI GGWCC 2 cut(s) 58, 214
ZraI GACGTC 1 cut(s) 130
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.