MD06G1194600.v1.1

HNH nucleases

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0005496
Physical Location & Seq
Reverse (-)
32929571 .. 32932081
2511 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1194600.v1.1.491

Sequence Viewer

Length: 609 bp
ATGAGGCCGAGCAAAGAGTCAAAGAGGCGGAGCCGCCCTTCGTCTCCACTCAGGGACCGAACCGCCGCGCCCTCCGCCCGTTTCGAACGACGTAGCAGCTCCCCACCGAAGCCTCGCGCCTCCGCTCTTCTCAATCGGGACCTCGTCGTCACCGACGTCACTGCCACTACTCGCTTCGAAGAAGAGCTCGCGACTTCTTCTTCTTTTAATAACAACCCACGAAGCTTTCCGTACAGCGTGAAGCAGCAGTGCTGGGACAAGGCGGAGAAGATCAAAGGTCGAGACCCGGACCGGTGGCGCCGTGACCCTCTCGGAAACACCATTTTTCGGAAGCTCGTGGGTTGCCCCGGTTGCTTATGTCATGATTATGATCACATTGTGCCTTACTCCAAGTTTTGTGAGGGAGGACAAAGTACGCTCGAAAATTGCCAGGTCTTACAGGCAACAGTCAATCGATCAAAAGGAAATCGGACTGAGTTTTCTAGAGCCGAGCTCATCCAGAAGAGTTCTTATTGTCGGGTTTCAGGTCGTGACATGGATCTTCTCGAACTGTCAGCCTATGGCAATGTCCGTCGTGGACAAGATTCAGGGGGATGTAGAATCCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HNH

Protein Analysis

203

Amino Acids

22.89

Weight (kDa)

9.78

Isoelectric Point (pI)

67.29

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 146
AatII GACGTC 1 cut(s) 159
AccB1I GGYRCC 1 cut(s) 297
AccBSI CCGCTC 1 cut(s) 125
AccII CGCG 3 cut(s) 68, 117, 191
AciI CCGC 7 cut(s) 28, 34, 63, 66, 75, 123, 263
AclWI GGATC 1 cut(s) 546
AcyI GRCGYC 2 cut(s) 156, 298
AdeI CACNNNGTG 1 cut(s) 379
AfaI GTAC 2 cut(s) 233, 415
AfiI CCNNNNNNNGG 1 cut(s) 327
AgeI ACCGGT 1 cut(s) 291
AjnI CCWGG 1 cut(s) 429
AluBI AGCT 5 cut(s) 99, 187, 225, 334, 493
AluI AGCT 5 cut(s) 99, 187, 225, 334, 493
Alw21I GWGCWC 2 cut(s) 189, 495
Alw26I GTCTC 2 cut(s) 48, 276
AlwI GGATC 1 cut(s) 546
AoxI GGCC 1 cut(s) 5
ApeKI GCWGC 2 cut(s) 96, 244
AsiGI ACCGGT 1 cut(s) 291
AspLEI GCGC 3 cut(s) 70, 119, 300
AspS9I GGNCC 3 cut(s) 55, 139, 289
AsuC2I CCSGG 2 cut(s) 287, 348
AsuHPI GGTGA 1 cut(s) 142
AsuII TTCGAA 2 cut(s) 84, 177
AvaII GGWCC 3 cut(s) 55, 139, 289
BanI GGYRCC 1 cut(s) 297
BanII GRGCYC 2 cut(s) 189, 495
BauI CACGAG 1 cut(s) 335
Bbv12I GWGCWC 2 cut(s) 189, 495
BbvI GCAGC 2 cut(s) 108, 256
BceAI ACGGC 1 cut(s) 285
BcgI CGANNNNNNTGC 2 cut(s) 143, 177
BciT130I CCWGG 1 cut(s) 431
BclI TGATCA 1 cut(s) 370
BcnI CCSGG 2 cut(s) 287, 348
BcoDI GTCTC 2 cut(s) 48, 276
BfaI CTAG 1 cut(s) 483
BfoI RGCGCY 1 cut(s) 301
BisI GCNGC 4 cut(s) 34, 66, 97, 245
BlsI GCNGC 4 cut(s) 35, 67, 98, 246
Bme1390I CCNGG 3 cut(s) 287, 348, 431
Bme18I GGWCC 3 cut(s) 55, 139, 289
BmgT120I GGNCC 3 cut(s) 55, 139, 289
BmiI GGNNCC 4 cut(s) 32, 56, 140, 299
BmrFI CCNGG 3 cut(s) 287, 348, 431
BplI GAGNNNNNCTC 2 cut(s) 477, 509
Bpu14I TTCGAA 2 cut(s) 84, 177
BpuMI CCSGG 2 cut(s) 287, 348
Bsa29I ATCGAT 1 cut(s) 454
BsaBI GATNNNNATC 1 cut(s) 369
BsaHI GRCGYC 2 cut(s) 156, 298
BsaI GGTCTC 1 cut(s) 276
BsaJI CCNNGG 1 cut(s) 346
BsaWI WCCGGW 1 cut(s) 291
Bsc4I CCNNNNNNNGG 1 cut(s) 327
Bse118I RCCGGY 1 cut(s) 291
Bse3DI GCAATG 1 cut(s) 571
Bse8I GATNNNNATC 1 cut(s) 369
BseBI CCWGG 1 cut(s) 431
BseCI ATCGAT 1 cut(s) 454
BseDI CCNNGG 1 cut(s) 346
BseGI GGATG 2 cut(s) 495, 599
BseJI GATNNNNATC 1 cut(s) 369
BseLI CCNNNNNNNGG 1 cut(s) 327
BseMI GCAATG 1 cut(s) 571
BseMII CTCAG 2 cut(s) 64, 465
BseXI GCAGC 2 cut(s) 108, 256
BseYI CCCAGC 1 cut(s) 252
Bsh1236I CGCG 3 cut(s) 68, 117, 191
BshFI GGCC 1 cut(s) 7
BshNI GGYRCC 1 cut(s) 297
BshTI ACCGGT 1 cut(s) 291
BshVI ATCGAT 1 cut(s) 454
BsiHKAI GWGCWC 2 cut(s) 189, 495
BsiSI CCGG 3 cut(s) 287, 292, 348
BslFI GGGAC 3 cut(s) 68, 152, 269
BslI CCNNNNNNNGG 1 cut(s) 327
BsmAI GTCTC 2 cut(s) 48, 276
BsmBI CGTCTC 1 cut(s) 48
BsmFI GGGAC 3 cut(s) 68, 152, 269
BsnI GGCC 1 cut(s) 7
Bso31I GGTCTC 1 cut(s) 276
Bsp119I TTCGAA 2 cut(s) 84, 177
Bsp1286I GDGCHC 2 cut(s) 189, 495
Bsp143I GATC 4 cut(s) 270, 370, 455, 538
Bsp68I TCGCGA 1 cut(s) 191
BspACI CCGC 7 cut(s) 28, 34, 63, 66, 75, 123, 263
BspANI GGCC 1 cut(s) 7
BspCNI CTCAG 2 cut(s) 63, 466
BspDI ATCGAT 1 cut(s) 454
BspFNI CGCG 3 cut(s) 68, 117, 191
BspHI TCATGA 1 cut(s) 361
BspLI GGNNCC 4 cut(s) 32, 56, 140, 299
BspPI GGATC 1 cut(s) 546
BspQI GCTCTTC 2 cut(s) 132, 177
BspT104I TTCGAA 2 cut(s) 84, 177
BspT107I GGYRCC 1 cut(s) 297
BspTNI GGTCTC 1 cut(s) 276
BsrBI CCGCTC 1 cut(s) 125
BsrDI GCAATG 1 cut(s) 571
BsrFI RCCGGY 1 cut(s) 291
BssAI RCCGGY 1 cut(s) 291
BssECI CCNNGG 1 cut(s) 346
BssMI GATC 4 cut(s) 270, 370, 455, 538
BssNI GRCGYC 2 cut(s) 156, 298
BssSI CACGAG 1 cut(s) 335
Bst2BI CACGAG 1 cut(s) 335
Bst2UI CCWGG 1 cut(s) 431
Bst4CI ACNGT 2 cut(s) 448, 552
Bst6I CTCTTC 3 cut(s) 132, 177, 497
BstACI GRCGYC 2 cut(s) 156, 298
BstBI TTCGAA 2 cut(s) 84, 177
BstC8I GCNNGC 1 cut(s) 189
BstDEI CTNAG 2 cut(s) 50, 474
BstF5I GGATG 2 cut(s) 495, 599
BstFNI CGCG 3 cut(s) 68, 117, 191
BstH2I RGCGCY 1 cut(s) 301
BstHHI GCGC 3 cut(s) 70, 119, 300
BstKTI GATC 4 cut(s) 273, 373, 458, 541
BstMAI GTCTC 2 cut(s) 48, 276
BstMBI GATC 4 cut(s) 270, 370, 455, 538
BstMWI GCNNNNNNNGC 2 cut(s) 74, 351
BstNI CCWGG 1 cut(s) 431
BstSCI CCNGG 3 cut(s) 285, 346, 429
BstUI CGCG 3 cut(s) 68, 117, 191
BstV1I GCAGC 2 cut(s) 108, 256
BstX2I RGATCY 1 cut(s) 538
BstYI RGATCY 1 cut(s) 538
Bsu15I ATCGAT 1 cut(s) 454
BsuRI GGCC 1 cut(s) 7
BsuTUI ATCGAT 1 cut(s) 454
BtsCI GGATG 2 cut(s) 495, 599
BtsI GCAGTG 2 cut(s) 159, 254
BtsIMutI CAGTG 2 cut(s) 159, 254
BtuMI TCGCGA 1 cut(s) 191
Cac8I GCNNGC 1 cut(s) 189
CciI TCATGA 1 cut(s) 361
CfoI GCGC 3 cut(s) 70, 119, 300
Cfr10I RCCGGY 1 cut(s) 291
Cfr13I GGNCC 3 cut(s) 55, 139, 289
ClaI ATCGAT 1 cut(s) 454
CpoI CGGWCCG 1 cut(s) 289
Csp6I GTAC 2 cut(s) 232, 414
CspAI ACCGGT 1 cut(s) 291
CspI CGGWCCG 1 cut(s) 289
CviAII CATG 2 cut(s) 362, 535
CviQI GTAC 2 cut(s) 232, 414
DdeI CTNAG 2 cut(s) 50, 474
DinI GGCGCC 1 cut(s) 299
DpnI GATC 4 cut(s) 272, 372, 457, 540
DpnII GATC 4 cut(s) 270, 370, 455, 538
DraIII CACNNNGTG 1 cut(s) 379
DrdI GACNNNNNNGTC 1 cut(s) 146
DseDI GACNNNNNNGTC 1 cut(s) 146
Eam1104I CTCTTC 3 cut(s) 132, 177, 497
EarI CTCTTC 3 cut(s) 132, 177, 497
EciI GGCGGA 3 cut(s) 43, 64, 278
Ecl136II GAGCTC 2 cut(s) 187, 493
Eco24I GRGCYC 2 cut(s) 189, 495
Eco31I GGTCTC 1 cut(s) 276
Eco47I GGWCC 3 cut(s) 55, 139, 289
Eco53kI GAGCTC 2 cut(s) 187, 493
EcoICRI GAGCTC 2 cut(s) 187, 493
EcoO109I RGGNCCY 1 cut(s) 139
EcoRII CCWGG 1 cut(s) 429
EcoT38I GRGCYC 2 cut(s) 189, 495
EgeI GGCGCC 1 cut(s) 299
EheI GGCGCC 1 cut(s) 299
Esp3I CGTCTC 1 cut(s) 48
FaeI CATG 2 cut(s) 365, 538
FaiI YATR 5 cut(s) 358, 363, 369, 536, 561
FaqI GGGAC 3 cut(s) 68, 152, 269
FatI CATG 2 cut(s) 361, 534
FbaI TGATCA 1 cut(s) 370
Fnu4HI GCNGC 4 cut(s) 34, 66, 97, 245
FokI GGATG 1 cut(s) 482
FriOI GRGCYC 2 cut(s) 189, 495
Fsp4HI GCNGC 4 cut(s) 34, 66, 97, 245
FspBI CTAG 1 cut(s) 483
GlaI GCGC 3 cut(s) 69, 118, 299
GluI GCNGC 4 cut(s) 34, 66, 97, 245
GsaI CCCAGC 1 cut(s) 256
HaeII RGCGCY 1 cut(s) 301
HaeIII GGCC 1 cut(s) 7
HapII CCGG 3 cut(s) 287, 292, 348
HhaI GCGC 3 cut(s) 70, 119, 300
Hin1I GRCGYC 2 cut(s) 156, 298
Hin1II CATG 2 cut(s) 365, 538
Hin6I GCGC 3 cut(s) 68, 117, 298
HinP1I GCGC 3 cut(s) 68, 117, 298
HindIII AAGCTT 1 cut(s) 223
HinfI GANTC 3 cut(s) 17, 584, 600
HpaII CCGG 3 cut(s) 287, 292, 348
HphI GGTGA 1 cut(s) 142
Hpy166II GTNNAC 1 cut(s) 578
Hpy188I TCNGA 3 cut(s) 314, 330, 471
Hpy188III TCNNGA 8 cut(s) 137, 190, 281, 362, 483, 499, 530, 545
Hpy8I GTNNAC 1 cut(s) 578
Hpy99I CGWCG 4 cut(s) 93, 149, 158, 576
HpyAV CCTTC 1 cut(s) 48
HpyCH4III ACNGT 2 cut(s) 448, 552
HpyCH4IV ACGT 2 cut(s) 91, 156
HpyF10VI GCNNNNNNNGC 2 cut(s) 74, 351
HpyF3I CTNAG 2 cut(s) 50, 474
HpySE526I ACGT 2 cut(s) 91, 156
Hsp92I GRCGYC 2 cut(s) 156, 298
Hsp92II CATG 2 cut(s) 365, 538
HspAI GCGC 3 cut(s) 68, 117, 298
KasI GGCGCC 1 cut(s) 297
Ksp22I TGATCA 1 cut(s) 370
Kzo9I GATC 4 cut(s) 270, 370, 455, 538
LguI GCTCTTC 2 cut(s) 132, 177
LmnI GCTCC 2 cut(s) 30, 104
Lsp1109I GCAGC 2 cut(s) 108, 256
MaeI CTAG 1 cut(s) 483
MaeII ACGT 2 cut(s) 91, 156
MaeIII GTNAC 4 cut(s) 148, 157, 302, 530
MalI GATC 4 cut(s) 272, 372, 457, 540
MbiI CCGCTC 1 cut(s) 125
MboI GATC 4 cut(s) 270, 370, 455, 538
MboII GAAGA 8 cut(s) 119, 189, 191, 192, 194, 280, 514, 533
MflI RGATCY 1 cut(s) 538
MhlI GDGCHC 2 cut(s) 189, 495
MluCI AATT 1 cut(s) 424
Mly113I GGCGCC 1 cut(s) 298
MlyI GAGTC 1 cut(s) 26
MnlI CCTC 8 cut(s) 18, 82, 123, 130, 152, 318, 394, 398
MseI TTAA 1 cut(s) 207
MslI CAYNNNNRTG 1 cut(s) 366
MspI CCGG 3 cut(s) 287, 292, 348
MspR9I CCNGG 3 cut(s) 287, 348, 431
MvaI CCWGG 1 cut(s) 431
MvnI CGCG 3 cut(s) 68, 117, 191
MwoI GCNNNNNNNGC 2 cut(s) 74, 351
NarI GGCGCC 1 cut(s) 298
NciI CCSGG 2 cut(s) 287, 348
NdeII GATC 4 cut(s) 270, 370, 455, 538
NlaIII CATG 2 cut(s) 365, 538
NlaIV GGNNCC 4 cut(s) 32, 56, 140, 299
NmeAIII GCCGAG 2 cut(s) 33, 514
NmuCI GTSAC 4 cut(s) 148, 157, 302, 530
NruI TCGCGA 1 cut(s) 191
NspV TTCGAA 2 cut(s) 84, 177
PagI TCATGA 1 cut(s) 361
PciSI GCTCTTC 2 cut(s) 132, 177
PcsI WCGNNNNNNNCGW 2 cut(s) 150, 153
PfeI GAWTC 2 cut(s) 584, 600
PflFI GACNNNGTC 1 cut(s) 143
PinAI ACCGGT 1 cut(s) 291
PkrI GCNGC 4 cut(s) 35, 67, 98, 246
PleI GAGTC 1 cut(s) 25
PluTI GGCGCC 1 cut(s) 301
PpsI GAGTC 1 cut(s) 25
PpuMI RGGWCCY 1 cut(s) 139
Psp124BI GAGCTC 2 cut(s) 189, 495
Psp5II RGGWCCY 1 cut(s) 139
Psp6I CCWGG 1 cut(s) 429
PspFI CCCAGC 1 cut(s) 252
PspGI CCWGG 1 cut(s) 429
PspN4I GGNNCC 4 cut(s) 32, 56, 140, 299
PspPI GGNCC 3 cut(s) 55, 139, 289
PspPPI RGGWCCY 1 cut(s) 139
PsuI RGATCY 1 cut(s) 538
PsyI GACNNNGTC 1 cut(s) 143
RruI TCGCGA 1 cut(s) 191
RsaI GTAC 2 cut(s) 233, 415
RsaNI GTAC 2 cut(s) 232, 414
RseI CAYNNNNRTG 1 cut(s) 366
Rsr2I CGGWCCG 1 cut(s) 289
RsrII CGGWCCG 1 cut(s) 289
SacI GAGCTC 2 cut(s) 189, 495
SapI GCTCTTC 2 cut(s) 132, 177
SaqAI TTAA 1 cut(s) 207
SatI GCNGC 4 cut(s) 34, 66, 97, 245
Sau3AI GATC 4 cut(s) 270, 370, 455, 538
Sau96I GGNCC 3 cut(s) 55, 139, 289
SchI GAGTC 1 cut(s) 26
ScrFI CCNGG 3 cut(s) 287, 348, 431
SduI GDGCHC 2 cut(s) 189, 495
SfoI GGCGCC 1 cut(s) 299
SfuI TTCGAA 2 cut(s) 84, 177
SinI GGWCC 3 cut(s) 55, 139, 289
SmiMI CAYNNNNRTG 1 cut(s) 366
Sse9I AATT 1 cut(s) 424
SsiI CCGC 7 cut(s) 28, 34, 63, 66, 75, 123, 263
SspDI GGCGCC 1 cut(s) 297
SspMI CTAG 1 cut(s) 483
SstI GAGCTC 2 cut(s) 189, 495
StyD4I CCNGG 3 cut(s) 285, 346, 429
TaaI ACNGT 2 cut(s) 448, 552
TaiI ACGT 2 cut(s) 94, 159
TaqI TCGA 6 cut(s) 84, 177, 280, 420, 454, 546
TaqII GACCGA 1 cut(s) 72
TasI AATT 1 cut(s) 424
TauI GCSGC 2 cut(s) 36, 68
TfiI GAWTC 2 cut(s) 584, 600
Tru1I TTAA 1 cut(s) 207
Tru9I TTAA 1 cut(s) 207
TscAI CASTG 2 cut(s) 166, 254
TseFI GTSAC 4 cut(s) 148, 157, 302, 530
TseI GCWGC 2 cut(s) 96, 244
Tsp45I GTSAC 4 cut(s) 148, 157, 302, 530
TspGWI ACGGA 2 cut(s) 219, 560
TspRI CASTG 2 cut(s) 166, 254
Tth111I GACNNNGTC 1 cut(s) 143
VpaK11BI GGWCC 3 cut(s) 55, 139, 289
XbaI TCTAGA 1 cut(s) 482
XspI CTAG 1 cut(s) 483
ZraI GACGTC 1 cut(s) 157
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.