RLG00000005182

HNH nucleases

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0005496
Physical Location & Seq
Forward (+)
65686205 .. 65689629
3425 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005182

Sequence Viewer

Length: 687 bp
ATGAGGCCAGCAAGCAAAGAACCAAAGCGGCGTAGCCACCCTTCATCTCCGTTGAGGGACCGAAACCCAACCTCGCCGCCCAGGTCTCGTTTCTCACCACCACGAGTCTCTCTTCTCGATGACGTCACCGACGACTTCGCTAACAACCCACGAAGCTTCCCGCACAGCGTGAAGAAACAATGCTGGGAGAAGGCGGAGAAGATCAAAGGGCGGGACCCAGATCGCTGGCGGCGGGACCCGCTCGGGAACACCGTGTTCCGGAAGCTCGTCGGCTGCCCCGGTTGCTTATGTCATGATTACGATCACATTGTGCCTTACTCTAAGGGTGGAGAAAGTACGCTTGAAAATTGTCAGGTTTTACAGGCAAAAGTGAACAGATCAAAAGGAAATCGAACAGAGTATTCTAGAGCTGAGCTCATTCAGAAAAGTTCTTACTGTCGGGTTTCAGGTCGCGATATGGATCTAATTGAACTGTCAGCCTATGGCAATGTCCATCCTGCACGAGAAGATTCATACTGTCCAGTTCCAGGTCGCGGTGTTGATCTGATTGACTTGTCAGCCAAAGGCAACATCCGTAATGCACAAGAGTTTTGGGGATACGAGCTGTATGAGACAAATGGACAGTCCATTTGCAAATCCTCACGCACAAGGTTTGTCACTAGGAGAGAGTTATCTACCATAACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HNH

Protein Analysis

229

Amino Acids

26.0

Weight (kDa)

9.47

Isoelectric Point (pI)

56.93

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 126
AccBSI CCGCTC 1 cut(s) 241
AccII CGCG 2 cut(s) 453, 534
AccIII TCCGGA 1 cut(s) 258
AciI CCGC 9 cut(s) 28, 77, 161, 194, 211, 229, 232, 239, 534
AclWI GGATC 1 cut(s) 468
AcyI GRCGYC 1 cut(s) 123
AdeI CACNNNGTG 2 cut(s) 169, 310
AfaI GTAC 1 cut(s) 337
AfiI CCNNNNNNNGG 3 cut(s) 258, 527, 533
AgsI TTSAA 2 cut(s) 344, 470
AjnI CCWGG 2 cut(s) 80, 526
AluBI AGCT 5 cut(s) 156, 265, 410, 415, 604
AluI AGCT 5 cut(s) 156, 265, 410, 415, 604
Alw21I GWGCWC 1 cut(s) 417
Alw26I GTCTC 3 cut(s) 90, 112, 605
AlwI GGATC 1 cut(s) 468
Ama87I CYCGRG 1 cut(s) 242
Aor13HI TCCGGA 1 cut(s) 258
AoxI GGCC 1 cut(s) 5
ApeKI GCWGC 1 cut(s) 273
ArsI GACNNNNNNTTYG 4 cut(s) 608, 612, 640, 644
AspS9I GGNCC 3 cut(s) 58, 214, 235
AsuC2I CCSGG 1 cut(s) 279
AsuHPI GGTGA 2 cut(s) 87, 118
AvaI CYCGRG 1 cut(s) 242
AvaII GGWCC 3 cut(s) 58, 214, 235
BanII GRGCYC 1 cut(s) 417
BarI GAAGNNNNNNTAC 2 cut(s) 25, 57
BauI CACGAG 2 cut(s) 102, 501
Bbv12I GWGCWC 1 cut(s) 417
BbvI GCAGC 1 cut(s) 260
BccI CCATC 1 cut(s) 501
BciT130I CCWGG 2 cut(s) 82, 528
BciVI GTATCC 1 cut(s) 590
BcnI CCSGG 1 cut(s) 279
BcoDI GTCTC 3 cut(s) 90, 112, 605
BfaI CTAG 2 cut(s) 405, 660
BfuI GTATCC 1 cut(s) 590
BisI GCNGC 4 cut(s) 29, 77, 230, 274
BlpI GCTNAGC 1 cut(s) 411
BlsI GCNGC 4 cut(s) 30, 78, 231, 275
Bme1390I CCNGG 3 cut(s) 82, 279, 528
Bme18I GGWCC 3 cut(s) 58, 214, 235
BmeT110I CYCGRG 1 cut(s) 242
BmgT120I GGNCC 3 cut(s) 58, 214, 235
BmiI GGNNCC 5 cut(s) 59, 215, 216, 236, 237
BmrFI CCNGG 3 cut(s) 82, 279, 528
BplI GAGNNNNNCTC 2 cut(s) 399, 431
Bpu1102I GCTNAGC 1 cut(s) 411
BpuMI CCSGG 1 cut(s) 279
BsaBI GATNNNNATC 2 cut(s) 300, 459
BsaHI GRCGYC 1 cut(s) 123
BsaI GGTCTC 1 cut(s) 90
BsaJI CCNNGG 2 cut(s) 80, 277
BsaWI WCCGGW 1 cut(s) 258
Bsc4I CCNNNNNNNGG 3 cut(s) 258, 527, 533
Bse1I ACTGG 1 cut(s) 521
Bse3DI GCAATG 1 cut(s) 493
Bse8I GATNNNNATC 2 cut(s) 300, 459
BseAI TCCGGA 1 cut(s) 258
BseBI CCWGG 2 cut(s) 82, 528
BseDI CCNNGG 2 cut(s) 80, 277
BseGI GGATG 2 cut(s) 493, 570
BseJI GATNNNNATC 2 cut(s) 300, 459
BseLI CCNNNNNNNGG 3 cut(s) 258, 527, 533
BseMI GCAATG 1 cut(s) 493
BseMII CTCAG 1 cut(s) 402
BseNI ACTGG 1 cut(s) 521
BseXI GCAGC 1 cut(s) 260
BseYI CCCAGC 1 cut(s) 183
BsgI GTGCAG 1 cut(s) 483
Bsh1236I CGCG 2 cut(s) 453, 534
BshFI GGCC 1 cut(s) 7
BsiHKAI GWGCWC 1 cut(s) 417
BsiHKCI CYCGRG 1 cut(s) 242
BsiSI CCGG 2 cut(s) 259, 279
BslFI GGGAC 3 cut(s) 71, 227, 248
BslI CCNNNNNNNGG 3 cut(s) 258, 527, 533
BsmAI GTCTC 3 cut(s) 90, 112, 605
BsmFI GGGAC 3 cut(s) 71, 227, 248
BsnI GGCC 1 cut(s) 7
Bso31I GGTCTC 1 cut(s) 90
BsoBI CYCGRG 1 cut(s) 242
Bsp1286I GDGCHC 1 cut(s) 417
Bsp13I TCCGGA 1 cut(s) 258
Bsp143I GATC 6 cut(s) 201, 220, 301, 377, 460, 541
Bsp1720I GCTNAGC 1 cut(s) 411
Bsp68I TCGCGA 1 cut(s) 453
BspACI CCGC 9 cut(s) 28, 77, 161, 194, 211, 229, 232, 239, 534
BspANI GGCC 1 cut(s) 7
BspCNI CTCAG 1 cut(s) 403
BspEI TCCGGA 1 cut(s) 258
BspFNI CGCG 2 cut(s) 453, 534
BspHI TCATGA 1 cut(s) 292
BspLI GGNNCC 5 cut(s) 59, 215, 216, 236, 237
BspPI GGATC 1 cut(s) 468
BspTNI GGTCTC 1 cut(s) 90
BsrBI CCGCTC 1 cut(s) 241
BsrDI GCAATG 1 cut(s) 493
BsrI ACTGG 1 cut(s) 521
BssECI CCNNGG 2 cut(s) 80, 277
BssMI GATC 6 cut(s) 201, 220, 301, 377, 460, 541
BssNI GRCGYC 1 cut(s) 123
BssSI CACGAG 2 cut(s) 102, 501
Bst2BI CACGAG 2 cut(s) 102, 501
Bst2UI CCWGG 2 cut(s) 82, 528
Bst4CI ACNGT 5 cut(s) 253, 437, 474, 518, 624
Bst6I CTCTTC 1 cut(s) 117
BstACI GRCGYC 1 cut(s) 123
BstC8I GCNNGC 3 cut(s) 9, 13, 227
BstDEI CTNAG 2 cut(s) 321, 411
BstF5I GGATG 2 cut(s) 493, 570
BstFNI CGCG 2 cut(s) 453, 534
BstKTI GATC 6 cut(s) 204, 223, 304, 380, 463, 544
BstMAI GTCTC 3 cut(s) 90, 112, 605
BstMBI GATC 6 cut(s) 201, 220, 301, 377, 460, 541
BstMWI GCNNNNNNNGC 2 cut(s) 238, 282
BstNI CCWGG 2 cut(s) 82, 528
BstSCI CCNGG 3 cut(s) 80, 277, 526
BstUI CGCG 2 cut(s) 453, 534
BstV1I GCAGC 1 cut(s) 260
BstX2I RGATCY 1 cut(s) 460
BstXI CCANNNNNNTGG 1 cut(s) 225
BstYI RGATCY 1 cut(s) 460
BsuI GTATCC 1 cut(s) 590
BsuRI GGCC 1 cut(s) 7
BtsCI GGATG 2 cut(s) 493, 570
BtuMI TCGCGA 1 cut(s) 453
Cac8I GCNNGC 3 cut(s) 9, 13, 227
CciI TCATGA 1 cut(s) 292
Cfr13I GGNCC 3 cut(s) 58, 214, 235
Csp6I GTAC 1 cut(s) 336
CviAII CATG 2 cut(s) 293, 684
CviQI GTAC 1 cut(s) 336
DdeI CTNAG 2 cut(s) 321, 411
DpnI GATC 6 cut(s) 203, 222, 303, 379, 462, 543
DpnII GATC 6 cut(s) 201, 220, 301, 377, 460, 541
DraIII CACNNNGTG 2 cut(s) 169, 310
Eam1104I CTCTTC 1 cut(s) 117
EarI CTCTTC 1 cut(s) 117
EciI GGCGGA 1 cut(s) 209
Ecl136II GAGCTC 1 cut(s) 415
Eco24I GRGCYC 1 cut(s) 417
Eco31I GGTCTC 1 cut(s) 90
Eco47I GGWCC 3 cut(s) 58, 214, 235
Eco53kI GAGCTC 1 cut(s) 415
Eco88I CYCGRG 1 cut(s) 242
EcoICRI GAGCTC 1 cut(s) 415
EcoO109I RGGNCCY 2 cut(s) 214, 235
EcoRII CCWGG 2 cut(s) 80, 526
EcoT38I GRGCYC 1 cut(s) 417
FaeI CATG 2 cut(s) 296, 687
FaiI YATR 8 cut(s) 289, 294, 458, 483, 514, 609, 680, 685
FaqI GGGAC 3 cut(s) 71, 227, 248
FatI CATG 2 cut(s) 292, 683
FauI CCCGC 4 cut(s) 168, 204, 225, 246
Fnu4HI GCNGC 4 cut(s) 29, 77, 230, 274
FokI GGATG 2 cut(s) 480, 557
FriOI GRGCYC 1 cut(s) 417
Fsp4HI GCNGC 4 cut(s) 29, 77, 230, 274
FspBI CTAG 2 cut(s) 405, 660
GluI GCNGC 4 cut(s) 29, 77, 230, 274
GsaI CCCAGC 1 cut(s) 187
HaeIII GGCC 1 cut(s) 7
HapII CCGG 2 cut(s) 259, 279
Hin1I GRCGYC 1 cut(s) 123
Hin1II CATG 2 cut(s) 296, 687
HindIII AAGCTT 1 cut(s) 154
HinfI GANTC 2 cut(s) 105, 509
HpaII CCGG 2 cut(s) 259, 279
HphI GGTGA 2 cut(s) 87, 118
Hpy166II GTNNAC 1 cut(s) 373
Hpy188I TCNGA 2 cut(s) 423, 546
Hpy188III TCNNGA 6 cut(s) 116, 244, 259, 293, 405, 452
Hpy8I GTNNAC 1 cut(s) 373
Hpy99I CGWCG 2 cut(s) 134, 272
HpyAV CCTTC 2 cut(s) 51, 184
HpyCH4III ACNGT 5 cut(s) 253, 437, 474, 518, 624
HpyCH4IV ACGT 1 cut(s) 123
HpyCH4V TGCA 3 cut(s) 500, 581, 633
HpyF10VI GCNNNNNNNGC 2 cut(s) 238, 282
HpyF3I CTNAG 2 cut(s) 321, 411
HpySE526I ACGT 1 cut(s) 123
Hsp92I GRCGYC 1 cut(s) 123
Hsp92II CATG 2 cut(s) 296, 687
KflI GGGWCCC 2 cut(s) 214, 235
Kpn2I TCCGGA 1 cut(s) 258
Kzo9I GATC 6 cut(s) 201, 220, 301, 377, 460, 541
Lsp1109I GCAGC 1 cut(s) 260
MaeI CTAG 2 cut(s) 405, 660
MaeII ACGT 1 cut(s) 123
MaeIII GTNAC 2 cut(s) 124, 655
MalI GATC 6 cut(s) 203, 222, 303, 379, 462, 543
MbiI CCGCTC 1 cut(s) 241
MboI GATC 6 cut(s) 201, 220, 301, 377, 460, 541
MboII GAAGA 4 cut(s) 104, 184, 211, 518
MflI RGATCY 1 cut(s) 460
MhlI GDGCHC 1 cut(s) 417
MluCI AATT 2 cut(s) 346, 465
MlyI GAGTC 1 cut(s) 114
MnlI CCTC 3 cut(s) 48, 82, 649
MroI TCCGGA 1 cut(s) 258
MspI CCGG 2 cut(s) 259, 279
MspR9I CCNGG 3 cut(s) 82, 279, 528
MvaI CCWGG 2 cut(s) 82, 528
MvnI CGCG 2 cut(s) 453, 534
MwoI GCNNNNNNNGC 2 cut(s) 238, 282
NciI CCSGG 1 cut(s) 279
NdeII GATC 6 cut(s) 201, 220, 301, 377, 460, 541
NlaIII CATG 2 cut(s) 296, 687
NlaIV GGNNCC 5 cut(s) 59, 215, 216, 236, 237
NmuCI GTSAC 2 cut(s) 124, 655
NruI TCGCGA 1 cut(s) 453
PagI TCATGA 1 cut(s) 292
PcsI WCGNNNNNNNCGW 2 cut(s) 129, 249
PfeI GAWTC 1 cut(s) 509
PkrI GCNGC 4 cut(s) 30, 78, 231, 275
PleI GAGTC 1 cut(s) 113
PpsI GAGTC 1 cut(s) 113
PpuMI RGGWCCY 2 cut(s) 214, 235
Psp124BI GAGCTC 1 cut(s) 417
Psp5II RGGWCCY 2 cut(s) 214, 235
Psp6I CCWGG 2 cut(s) 80, 526
PspFI CCCAGC 1 cut(s) 183
PspGI CCWGG 2 cut(s) 80, 526
PspN4I GGNNCC 5 cut(s) 59, 215, 216, 236, 237
PspPI GGNCC 3 cut(s) 58, 214, 235
PspPPI RGGWCCY 2 cut(s) 214, 235
PsuI RGATCY 1 cut(s) 460
RruI TCGCGA 1 cut(s) 453
RsaI GTAC 1 cut(s) 337
RsaNI GTAC 1 cut(s) 336
SacI GAGCTC 1 cut(s) 417
SatI GCNGC 4 cut(s) 29, 77, 230, 274
Sau3AI GATC 6 cut(s) 201, 220, 301, 377, 460, 541
Sau96I GGNCC 3 cut(s) 58, 214, 235
SchI GAGTC 1 cut(s) 114
ScrFI CCNGG 3 cut(s) 82, 279, 528
SduI GDGCHC 1 cut(s) 417
SinI GGWCC 3 cut(s) 58, 214, 235
Sse9I AATT 2 cut(s) 346, 465
SsiI CCGC 9 cut(s) 28, 77, 161, 194, 211, 229, 232, 239, 534
SspMI CTAG 2 cut(s) 405, 660
SstI GAGCTC 1 cut(s) 417
StyD4I CCNGG 3 cut(s) 80, 277, 526
TaaI ACNGT 5 cut(s) 253, 437, 474, 518, 624
TaiI ACGT 1 cut(s) 126
TaqI TCGA 2 cut(s) 117, 391
TaqII GACCGA 1 cut(s) 75
TasI AATT 2 cut(s) 346, 465
TauI GCSGC 3 cut(s) 31, 79, 232
TfiI GAWTC 1 cut(s) 509
TseFI GTSAC 2 cut(s) 124, 655
TseI GCWGC 1 cut(s) 273
Tsp45I GTSAC 2 cut(s) 124, 655
TspDTI ATGAA 2 cut(s) 33, 501
TspGWI ACGGA 2 cut(s) 39, 563
VpaK11BI GGWCC 3 cut(s) 58, 214, 235
XbaI TCTAGA 1 cut(s) 404
XspI CTAG 2 cut(s) 405, 660
ZraI GACGTC 1 cut(s) 124
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.