pycom06g17230

HNH nucleases

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0005496
Physical Location & Seq
Reverse (-)
21242181 .. 21244460
2280 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g17230.2

Sequence Viewer

Length: 600 bp
ATGAGGCCGGGCAAAGAGCCAAAGAGGCGGAGCCGCCCTTCGTCTCCACTCAGGGACCGAACCGCCGCGCCCTCCGCCCGTTTCGAACGACGTAGCAGCTCACCACCGAAGCCTCGCGCCTCCGCTCTTCTCAATCGGGACCTCGTCGTCACCGACGTCACTGCCACTACTCGCTTCGAAGAAGAGCTCGCGACTTCTTCTTCTTTTAATAACAACCCACGAAGCTTTCCGTACAGCGTGAAGCAGCAGTGCTGGGACAAGGCGGAGAAGATCAAAGGTCGAGACCCGGACCGGTGGCGCCGTGACCCTCTCGGAAACACCATTTTTCGGAAGCTCGTGGGTTGCCCCGGTTGCTTATGTCATGATTATGATCACATTGTGCCTTACTCCAAGGGAGGACAAAGTACGCTCGAAAATTGCCAGGTCTTACAGGCAACAGTCAATCGATCAAAAGGAAATCGGACTGAGTTTTCTAGAGCCGAGCTCATCCAGAAGAGTTCTTACTGTCGGGTTTCAGGTCGTGACATGGATCTTCTCGAACTGTCAGCCTATGGCAACGTCCGTCGTGGACAAGATTCAGGGGGATGTAGAATCCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HNH

Protein Analysis

200

Amino Acids

22.49

Weight (kDa)

9.94

Isoelectric Point (pI)

66.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 146
AatII GACGTC 1 cut(s) 159
AccB1I GGYRCC 1 cut(s) 297
AccBSI CCGCTC 1 cut(s) 125
AccII CGCG 3 cut(s) 68, 117, 191
AciI CCGC 7 cut(s) 28, 34, 63, 66, 75, 123, 263
AclWI GGATC 1 cut(s) 537
AcyI GRCGYC 2 cut(s) 156, 298
AdeI CACNNNGTG 1 cut(s) 379
AfaI GTAC 2 cut(s) 233, 406
AfiI CCNNNNNNNGG 1 cut(s) 327
AgeI ACCGGT 1 cut(s) 291
AjnI CCWGG 1 cut(s) 420
AluBI AGCT 5 cut(s) 99, 187, 225, 334, 484
AluI AGCT 5 cut(s) 99, 187, 225, 334, 484
Alw21I GWGCWC 2 cut(s) 189, 486
Alw26I GTCTC 2 cut(s) 48, 276
AlwI GGATC 1 cut(s) 537
AoxI GGCC 1 cut(s) 5
ApeKI GCWGC 2 cut(s) 96, 244
AsiGI ACCGGT 1 cut(s) 291
AspLEI GCGC 3 cut(s) 70, 119, 300
AspS9I GGNCC 3 cut(s) 55, 139, 289
AsuC2I CCSGG 3 cut(s) 9, 287, 348
AsuHPI GGTGA 2 cut(s) 93, 142
AsuII TTCGAA 2 cut(s) 84, 177
AvaII GGWCC 3 cut(s) 55, 139, 289
BanI GGYRCC 1 cut(s) 297
BanII GRGCYC 2 cut(s) 189, 486
BarI GAAGNNNNNNTAC 2 cut(s) 485, 517
BauI CACGAG 1 cut(s) 335
Bbv12I GWGCWC 2 cut(s) 189, 486
BbvI GCAGC 2 cut(s) 108, 256
BceAI ACGGC 1 cut(s) 285
BcgI CGANNNNNNTGC 2 cut(s) 143, 177
BciT130I CCWGG 1 cut(s) 422
BclI TGATCA 1 cut(s) 370
BcnI CCSGG 3 cut(s) 9, 287, 348
BcoDI GTCTC 2 cut(s) 48, 276
BfaI CTAG 1 cut(s) 474
BfoI RGCGCY 1 cut(s) 301
BglI GCCNNNNNGGC 1 cut(s) 25
BisI GCNGC 4 cut(s) 34, 66, 97, 245
BlsI GCNGC 4 cut(s) 35, 67, 98, 246
Bme1390I CCNGG 4 cut(s) 9, 287, 348, 422
Bme18I GGWCC 3 cut(s) 55, 139, 289
BmgT120I GGNCC 3 cut(s) 55, 139, 289
BmiI GGNNCC 4 cut(s) 32, 56, 140, 299
BmrFI CCNGG 4 cut(s) 9, 287, 348, 422
BplI GAGNNNNNCTC 2 cut(s) 468, 500
Bpu14I TTCGAA 2 cut(s) 84, 177
BpuMI CCSGG 3 cut(s) 9, 287, 348
Bsa29I ATCGAT 1 cut(s) 445
BsaBI GATNNNNATC 1 cut(s) 369
BsaHI GRCGYC 2 cut(s) 156, 298
BsaI GGTCTC 1 cut(s) 276
BsaJI CCNNGG 2 cut(s) 346, 390
BsaWI WCCGGW 1 cut(s) 291
Bsc4I CCNNNNNNNGG 1 cut(s) 327
Bse118I RCCGGY 1 cut(s) 291
Bse8I GATNNNNATC 1 cut(s) 369
BseBI CCWGG 1 cut(s) 422
BseCI ATCGAT 1 cut(s) 445
BseDI CCNNGG 2 cut(s) 346, 390
BseGI GGATG 2 cut(s) 486, 590
BseJI GATNNNNATC 1 cut(s) 369
BseLI CCNNNNNNNGG 1 cut(s) 327
BseMII CTCAG 2 cut(s) 64, 456
BseXI GCAGC 2 cut(s) 108, 256
BseYI CCCAGC 1 cut(s) 252
Bsh1236I CGCG 3 cut(s) 68, 117, 191
BshFI GGCC 1 cut(s) 7
BshNI GGYRCC 1 cut(s) 297
BshTI ACCGGT 1 cut(s) 291
BshVI ATCGAT 1 cut(s) 445
BsiHKAI GWGCWC 2 cut(s) 189, 486
BsiSI CCGG 4 cut(s) 8, 287, 292, 348
BslFI GGGAC 3 cut(s) 68, 152, 269
BslI CCNNNNNNNGG 1 cut(s) 327
BsmAI GTCTC 2 cut(s) 48, 276
BsmBI CGTCTC 1 cut(s) 48
BsmFI GGGAC 3 cut(s) 68, 152, 269
BsnI GGCC 1 cut(s) 7
Bso31I GGTCTC 1 cut(s) 276
Bsp119I TTCGAA 2 cut(s) 84, 177
Bsp1286I GDGCHC 2 cut(s) 189, 486
Bsp143I GATC 4 cut(s) 270, 370, 446, 529
Bsp68I TCGCGA 1 cut(s) 191
BspACI CCGC 7 cut(s) 28, 34, 63, 66, 75, 123, 263
BspANI GGCC 1 cut(s) 7
BspCNI CTCAG 2 cut(s) 63, 457
BspDI ATCGAT 1 cut(s) 445
BspFNI CGCG 3 cut(s) 68, 117, 191
BspHI TCATGA 1 cut(s) 361
BspLI GGNNCC 4 cut(s) 32, 56, 140, 299
BspPI GGATC 1 cut(s) 537
BspQI GCTCTTC 2 cut(s) 132, 177
BspT104I TTCGAA 2 cut(s) 84, 177
BspT107I GGYRCC 1 cut(s) 297
BspTNI GGTCTC 1 cut(s) 276
BsrBI CCGCTC 1 cut(s) 125
BsrFI RCCGGY 1 cut(s) 291
BssAI RCCGGY 1 cut(s) 291
BssECI CCNNGG 2 cut(s) 346, 390
BssMI GATC 4 cut(s) 270, 370, 446, 529
BssNI GRCGYC 2 cut(s) 156, 298
BssSI CACGAG 1 cut(s) 335
BssT1I CCWWGG 1 cut(s) 390
Bst2BI CACGAG 1 cut(s) 335
Bst2UI CCWGG 1 cut(s) 422
Bst4CI ACNGT 3 cut(s) 439, 506, 543
Bst6I CTCTTC 3 cut(s) 132, 177, 488
BstACI GRCGYC 2 cut(s) 156, 298
BstBI TTCGAA 2 cut(s) 84, 177
BstC8I GCNNGC 1 cut(s) 189
BstDEI CTNAG 2 cut(s) 50, 465
BstF5I GGATG 2 cut(s) 486, 590
BstFNI CGCG 3 cut(s) 68, 117, 191
BstH2I RGCGCY 1 cut(s) 301
BstHHI GCGC 3 cut(s) 70, 119, 300
BstKTI GATC 4 cut(s) 273, 373, 449, 532
BstMAI GTCTC 2 cut(s) 48, 276
BstMBI GATC 4 cut(s) 270, 370, 446, 529
BstMWI GCNNNNNNNGC 3 cut(s) 25, 74, 351
BstNI CCWGG 1 cut(s) 422
BstSCI CCNGG 4 cut(s) 7, 285, 346, 420
BstUI CGCG 3 cut(s) 68, 117, 191
BstV1I GCAGC 2 cut(s) 108, 256
BstX2I RGATCY 1 cut(s) 529
BstYI RGATCY 1 cut(s) 529
Bsu15I ATCGAT 1 cut(s) 445
BsuRI GGCC 1 cut(s) 7
BsuTUI ATCGAT 1 cut(s) 445
BtsCI GGATG 2 cut(s) 486, 590
BtsI GCAGTG 2 cut(s) 159, 254
BtsIMutI CAGTG 2 cut(s) 159, 254
BtuMI TCGCGA 1 cut(s) 191
Cac8I GCNNGC 1 cut(s) 189
CciI TCATGA 1 cut(s) 361
CfoI GCGC 3 cut(s) 70, 119, 300
Cfr10I RCCGGY 1 cut(s) 291
Cfr13I GGNCC 3 cut(s) 55, 139, 289
ClaI ATCGAT 1 cut(s) 445
CpoI CGGWCCG 1 cut(s) 289
Csp6I GTAC 2 cut(s) 232, 405
CspAI ACCGGT 1 cut(s) 291
CspI CGGWCCG 1 cut(s) 289
CviAII CATG 2 cut(s) 362, 526
CviQI GTAC 2 cut(s) 232, 405
DdeI CTNAG 2 cut(s) 50, 465
DinI GGCGCC 1 cut(s) 299
DpnI GATC 4 cut(s) 272, 372, 448, 531
DpnII GATC 4 cut(s) 270, 370, 446, 529
DraIII CACNNNGTG 1 cut(s) 379
DrdI GACNNNNNNGTC 1 cut(s) 146
DseDI GACNNNNNNGTC 1 cut(s) 146
Eam1104I CTCTTC 3 cut(s) 132, 177, 488
EarI CTCTTC 3 cut(s) 132, 177, 488
EciI GGCGGA 3 cut(s) 43, 64, 278
Ecl136II GAGCTC 2 cut(s) 187, 484
Eco130I CCWWGG 1 cut(s) 390
Eco24I GRGCYC 2 cut(s) 189, 486
Eco31I GGTCTC 1 cut(s) 276
Eco47I GGWCC 3 cut(s) 55, 139, 289
Eco53kI GAGCTC 2 cut(s) 187, 484
EcoICRI GAGCTC 2 cut(s) 187, 484
EcoO109I RGGNCCY 1 cut(s) 139
EcoRII CCWGG 1 cut(s) 420
EcoT14I CCWWGG 1 cut(s) 390
EcoT38I GRGCYC 2 cut(s) 189, 486
EgeI GGCGCC 1 cut(s) 299
EheI GGCGCC 1 cut(s) 299
ErhI CCWWGG 1 cut(s) 390
Esp3I CGTCTC 1 cut(s) 48
FaeI CATG 2 cut(s) 365, 529
FaiI YATR 5 cut(s) 358, 363, 369, 527, 552
FaqI GGGAC 3 cut(s) 68, 152, 269
FatI CATG 2 cut(s) 361, 525
FbaI TGATCA 1 cut(s) 370
Fnu4HI GCNGC 4 cut(s) 34, 66, 97, 245
FokI GGATG 1 cut(s) 473
FriOI GRGCYC 2 cut(s) 189, 486
Fsp4HI GCNGC 4 cut(s) 34, 66, 97, 245
FspBI CTAG 1 cut(s) 474
GlaI GCGC 3 cut(s) 69, 118, 299
GluI GCNGC 4 cut(s) 34, 66, 97, 245
GsaI CCCAGC 1 cut(s) 256
HaeII RGCGCY 1 cut(s) 301
HaeIII GGCC 1 cut(s) 7
HapII CCGG 4 cut(s) 8, 287, 292, 348
HhaI GCGC 3 cut(s) 70, 119, 300
Hin1I GRCGYC 2 cut(s) 156, 298
Hin1II CATG 2 cut(s) 365, 529
Hin6I GCGC 3 cut(s) 68, 117, 298
HinP1I GCGC 3 cut(s) 68, 117, 298
HindIII AAGCTT 1 cut(s) 223
HinfI GANTC 2 cut(s) 575, 591
HpaII CCGG 4 cut(s) 8, 287, 292, 348
HphI GGTGA 2 cut(s) 93, 142
Hpy166II GTNNAC 1 cut(s) 569
Hpy188I TCNGA 3 cut(s) 314, 330, 462
Hpy188III TCNNGA 8 cut(s) 137, 190, 281, 362, 474, 490, 521, 536
Hpy8I GTNNAC 1 cut(s) 569
Hpy99I CGWCG 4 cut(s) 93, 149, 158, 567
HpyAV CCTTC 1 cut(s) 48
HpyCH4III ACNGT 3 cut(s) 439, 506, 543
HpyCH4IV ACGT 3 cut(s) 91, 156, 558
HpyF10VI GCNNNNNNNGC 3 cut(s) 25, 74, 351
HpyF3I CTNAG 2 cut(s) 50, 465
HpySE526I ACGT 3 cut(s) 91, 156, 558
Hsp92I GRCGYC 2 cut(s) 156, 298
Hsp92II CATG 2 cut(s) 365, 529
HspAI GCGC 3 cut(s) 68, 117, 298
KasI GGCGCC 1 cut(s) 297
Ksp22I TGATCA 1 cut(s) 370
Kzo9I GATC 4 cut(s) 270, 370, 446, 529
LguI GCTCTTC 2 cut(s) 132, 177
LmnI GCTCC 1 cut(s) 30
Lsp1109I GCAGC 2 cut(s) 108, 256
MaeI CTAG 1 cut(s) 474
MaeII ACGT 3 cut(s) 91, 156, 558
MaeIII GTNAC 4 cut(s) 148, 157, 302, 521
MalI GATC 4 cut(s) 272, 372, 448, 531
MbiI CCGCTC 1 cut(s) 125
MboI GATC 4 cut(s) 270, 370, 446, 529
MboII GAAGA 8 cut(s) 119, 189, 191, 192, 194, 280, 505, 524
MflI RGATCY 1 cut(s) 529
MhlI GDGCHC 2 cut(s) 189, 486
MluCI AATT 1 cut(s) 415
Mly113I GGCGCC 1 cut(s) 298
MnlI CCTC 7 cut(s) 18, 82, 123, 130, 152, 318, 389
MseI TTAA 1 cut(s) 207
MslI CAYNNNNRTG 1 cut(s) 366
MspI CCGG 4 cut(s) 8, 287, 292, 348
MspR9I CCNGG 4 cut(s) 9, 287, 348, 422
MvaI CCWGG 1 cut(s) 422
MvnI CGCG 3 cut(s) 68, 117, 191
MwoI GCNNNNNNNGC 3 cut(s) 25, 74, 351
NarI GGCGCC 1 cut(s) 298
NciI CCSGG 3 cut(s) 9, 287, 348
NdeII GATC 4 cut(s) 270, 370, 446, 529
NlaIII CATG 2 cut(s) 365, 529
NlaIV GGNNCC 4 cut(s) 32, 56, 140, 299
NmeAIII GCCGAG 1 cut(s) 505
NmuCI GTSAC 4 cut(s) 148, 157, 302, 521
NruI TCGCGA 1 cut(s) 191
NspV TTCGAA 2 cut(s) 84, 177
PagI TCATGA 1 cut(s) 361
PciSI GCTCTTC 2 cut(s) 132, 177
PcsI WCGNNNNNNNCGW 2 cut(s) 150, 153
PfeI GAWTC 2 cut(s) 575, 591
PflFI GACNNNGTC 1 cut(s) 143
PinAI ACCGGT 1 cut(s) 291
PkrI GCNGC 4 cut(s) 35, 67, 98, 246
PluTI GGCGCC 1 cut(s) 301
PpuMI RGGWCCY 1 cut(s) 139
Psp124BI GAGCTC 2 cut(s) 189, 486
Psp5II RGGWCCY 1 cut(s) 139
Psp6I CCWGG 1 cut(s) 420
PspFI CCCAGC 1 cut(s) 252
PspGI CCWGG 1 cut(s) 420
PspN4I GGNNCC 4 cut(s) 32, 56, 140, 299
PspPI GGNCC 3 cut(s) 55, 139, 289
PspPPI RGGWCCY 1 cut(s) 139
PsuI RGATCY 1 cut(s) 529
PsyI GACNNNGTC 1 cut(s) 143
RruI TCGCGA 1 cut(s) 191
RsaI GTAC 2 cut(s) 233, 406
RsaNI GTAC 2 cut(s) 232, 405
RseI CAYNNNNRTG 1 cut(s) 366
Rsr2I CGGWCCG 1 cut(s) 289
RsrII CGGWCCG 1 cut(s) 289
SacI GAGCTC 2 cut(s) 189, 486
SapI GCTCTTC 2 cut(s) 132, 177
SaqAI TTAA 1 cut(s) 207
SatI GCNGC 4 cut(s) 34, 66, 97, 245
Sau3AI GATC 4 cut(s) 270, 370, 446, 529
Sau96I GGNCC 3 cut(s) 55, 139, 289
ScrFI CCNGG 4 cut(s) 9, 287, 348, 422
SduI GDGCHC 2 cut(s) 189, 486
SfoI GGCGCC 1 cut(s) 299
SfuI TTCGAA 2 cut(s) 84, 177
SinI GGWCC 3 cut(s) 55, 139, 289
SmiMI CAYNNNNRTG 1 cut(s) 366
Sse9I AATT 1 cut(s) 415
SsiI CCGC 7 cut(s) 28, 34, 63, 66, 75, 123, 263
SspDI GGCGCC 1 cut(s) 297
SspMI CTAG 1 cut(s) 474
SstI GAGCTC 2 cut(s) 189, 486
StyD4I CCNGG 4 cut(s) 7, 285, 346, 420
StyI CCWWGG 1 cut(s) 390
TaaI ACNGT 3 cut(s) 439, 506, 543
TaiI ACGT 3 cut(s) 94, 159, 561
TaqI TCGA 6 cut(s) 84, 177, 280, 411, 445, 537
TaqII GACCGA 1 cut(s) 72
TasI AATT 1 cut(s) 415
TauI GCSGC 2 cut(s) 36, 68
TfiI GAWTC 2 cut(s) 575, 591
Tru1I TTAA 1 cut(s) 207
Tru9I TTAA 1 cut(s) 207
TscAI CASTG 2 cut(s) 166, 254
TseFI GTSAC 4 cut(s) 148, 157, 302, 521
TseI GCWGC 2 cut(s) 96, 244
Tsp45I GTSAC 4 cut(s) 148, 157, 302, 521
TspGWI ACGGA 2 cut(s) 219, 551
TspRI CASTG 2 cut(s) 166, 254
Tth111I GACNNNGTC 1 cut(s) 143
VpaK11BI GGWCC 3 cut(s) 55, 139, 289
XbaI TCTAGA 1 cut(s) 473
XspI CTAG 1 cut(s) 474
ZraI GACGTC 1 cut(s) 157
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.