FvH4_5g14900

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0016162
Physical Location & Seq
Reverse (-)
8436676 .. 8438607
1932 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g14900.t1

Sequence Viewer

Length: 1932 bp
ATGACTGCGATCTCACAGTCCCTGCGTTCAAACTGTGCCCATCTCCAACATCACAACATCTCCTCTACTCGTCTCACCTCCACCTTCCCCTCACTCTTCACCACTAACCAAACCTCCCTCGACCATTCCCAAATCCAAACCCCCCATGACCATCAAAACCAGACCAAACCCATCATCATCTCCTACGCTCAAACCCGAAAAGACTCCCTCCTCTCCCTCATCAAATCATGCACCCACAAATCCCATTTACTCCAAATCCACGCCCACATTCTCCAAACCTCTCTCATTCTCGACTCCTCTATCTGCTTCCACTTCCTCTCCCTCCTTTCGCTTTCCCCGCCACTCAAAAACTTAACCTACTCCCGCCATTTCTTAGCCCAAATCCCCAAACCCAATGCCATTCACTACAACACTCTCATAAGAGCTTACTCCACGAGCGATTCACCCGAACAGGGTATTCATCTCTACCGAGACTTCAGACGGCGAGGGCTTCATTGCAACTCGCTGTCGTCCTTCTTCGTAATCCAGTGCTGTGTTAAAATGCAATGCTTATCAGTAGGAATTCAGGTGCAAACTAGGATTGTTAGAGATGGGCATCATTCAGATAGTCGCTTACTCACAGCTCTGATGAACCTGTACTCGACTTGTGGGGAGTATCATGATGCATGTAAGGTGTTCGATGAAATTCCGCAGAGGGACACTGTTGCTTGGAATGTGTTGATTTCTTGCTTTCTGCATAACAGCCACAGCAGGGACGCTCTGGGTGTGTTTGACGTTATGCGGAGCGGAAGCTACGGATGTGAGCCTGATGATGTTACTTGTTTACTTACGCTGCAAGCTTGTGCAAACATGAACGCCTTGGAGTTTGGCGAAAGGGTTCATAGGTACATTGAGGAACATGGTTATGGTGGCGCTTCTAATCTGTGTAATGCTCTTATTACTATGTATTCGCGGTGTGGGTGTTTGGATAAGGCTTATGAGGTGTTTAAGGGAATGCGGGGTAGAAATGTGGTTTCATGGAGTGCAATGATTTCCGGGCTTGCAGTGAATGGGTATGGTAGAGACGCGGTTGAAGCGTTTTGTGAGATGCAGAGAATGGGTGTTTTACCTGATGAACAGACTTTTACTGGTGTGCTTTCTGCTTGCAGTCATTGTGGGTTGGTTGTTGAAGCAATGGATATTTTTGAGCGCATGAGTAAAGAGTTTGGAGTAGTGCCTAATATCCATCACTATGGGTGTATAGTTGATCTATTGGGTCGTGCTGGTCGACTTGATCAAGCTTATCAGCTTATAATGTCGATGGATATTAATCCAGATTCGAAAATATGGAGGACCTTACTTGGGGCTTGCAGGATTCATAACTATGAAACCCTTGGGGAACGTGTGGTGGATCATTTGATCGAACTCAAGGCTCAAGAGGCAGGGGACTATGTTCTGTTGATGAATATTTACTCTACAGCTAAAAACTGGGAGAAGTTAACTGAATTGAGGAAGTTTATGAAGGAAAATGCAATTCAAACTACACCAGGCTGCAGCACAATCATACTGGATGGAACAGTCCACGAGTTTTTGGTGGATGATGTTTCACATCCAAGGAAGGACGAAATCTACCGAATGCTAGATGAGATCAATAGTCAGCTGAAGATTGCTGGTTATGTTGCTGATGTATCATCTGAACTTCACAATTTGGGGGCAGCAGAGAAGGGATATGCACTCTCCTATCACAGCGAGAAACTAGCTATTGCTTTTGGAGTGCTGGCAACTCCACCTGGCATGACAATTAGAGTGGCCAAGAATCTTCGCACTTGTGTTGATTGCCACAACTTTGCAATGATTCTCTCAGGGGTTTATAACAGGACGATAATTGTTAGAGACCGCAGTCGGTTTCATCATTTTCGGGAGGGACGTTGCTCATGCAATGGCTATTGGTAG

Protein Analysis

644

Amino Acids

72.43

Weight (kDa)

7.19

Isoelectric Point (pI)

38.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 232 - 283 7.9e-08 PPR repeat family
PPR_2 PF13041 335 - 383 2.2e-11 PPR repeat family
PPR PF01535 338 - 368 6.5e-07 PPR repeat
E_motif PF20431 455 - 514 1.8e-14 E motif
DYW_deaminase PF14432 551 - 643 3.9e-34 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016162)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G47530
fragaria_vesca FvH4_5g14900
malus_domestica MD06G1193700.v1.1 MD14G1200800.v1.1
prunus_persica Prupe.5G194000_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0181481
rosa_laevigata RLG00000005190
rosa_multiflora Rmu_co8291233.1_g000001 Rmu_sc0002060.1_g000023
rosa_roxburghii Rroxscaffold_3G00271960
rosa_rugosa Rorug06G0441700
rosa_samantha Rh7AG043500 Rh7BG042900 Rh7CG044700
rosa_wichuraiana Rw7G003620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1292, 1851
AccBSI CCGCTC 1 cut(s) 786
AccI GTMKAC 1 cut(s) 1267
AccII CGCG 2 cut(s) 952, 1067
AciI CCGC 9 cut(s) 338, 364, 689, 781, 786, 952, 997, 1067, 1876
AclWI GGATC 1 cut(s) 1398
AcoI YGGCCR 1 cut(s) 1788
AcsI RAATTY 2 cut(s) 561, 684
AcuI CTGAAG 2 cut(s) 460, 1661
AfaI GTAC 2 cut(s) 638, 887
AfiI CCNNNNNNNGG 3 cut(s) 452, 751, 1341
AflIII ACRYGT 1 cut(s) 1381
AgsI TTSAA 4 cut(s) 30, 1073, 1169, 1517
AjnI CCWGG 2 cut(s) 1525, 1768
AluBI AGCT 9 cut(s) 425, 623, 792, 839, 1280, 1288, 1460, 1639, 1739
AluI AGCT 9 cut(s) 425, 623, 792, 839, 1280, 1288, 1460, 1639, 1739
Alw26I GTCTC 4 cut(s) 77, 465, 1056, 1866
AlwI GGATC 1 cut(s) 1398
AlwNI CAGNNNCTG 1 cut(s) 22
AoxI GGCC 1 cut(s) 1788
ApeKI GCWGC 4 cut(s) 832, 1530, 1533, 1694
ApoI RAATTY 2 cut(s) 561, 684
AseI ATTAAT 1 cut(s) 1308
Asp700I GAANNNNTTC 1 cut(s) 876
AspLEI GCGC 2 cut(s) 914, 1191
AspS9I GGNCC 1 cut(s) 1332
AsuC2I CCSGG 1 cut(s) 1036
AsuHPI GGTGA 3 cut(s) 67, 91, 435
AsuII TTCGAA 1 cut(s) 1319
AvaII GGWCC 1 cut(s) 1332
BaeGI GKGCMC 1 cut(s) 40
BalI TGGCCA 1 cut(s) 1790
BauI CACGAG 2 cut(s) 433, 1562
BbvI GCAGC 4 cut(s) 819, 1517, 1545, 1706
BccI CCATC 7 cut(s) 48, 159, 179, 584, 1233, 1294, 1544
BceAI ACGGC 1 cut(s) 497
BciT130I CCWGG 2 cut(s) 1527, 1770
BclI TGATCA 1 cut(s) 1273
BcnI CCSGG 1 cut(s) 1036
BcoDI GTCTC 4 cut(s) 77, 465, 1056, 1866
BfaI CTAG 3 cut(s) 576, 1619, 1736
BfmI CTRYAG 2 cut(s) 1455, 1531
BfoI RGCGCY 1 cut(s) 915
BisI GCNGC 4 cut(s) 833, 1531, 1534, 1695
BlsI GCNGC 4 cut(s) 834, 1532, 1535, 1696
Bme1390I CCNGG 3 cut(s) 1036, 1527, 1770
Bme18I GGWCC 1 cut(s) 1332
BmgT120I GGNCC 1 cut(s) 1332
BmrFI CCNGG 3 cut(s) 1036, 1527, 1770
BmrI ACTGGG 1 cut(s) 1477
BmsI GCATC 3 cut(s) 604, 652, 1077
BmuI ACTGGG 1 cut(s) 1477
BoxI GACNNNNGTC 1 cut(s) 1878
Bpu14I TTCGAA 1 cut(s) 1319
BpuEI CTTGAG 2 cut(s) 1391, 1398
BpuMI CCSGG 1 cut(s) 1036
BsaBI GATNNNNATC 2 cut(s) 594, 1308
BsaI GGTCTC 1 cut(s) 1866
BsaJI CCNNGG 3 cut(s) 858, 1372, 1592
BsaXI ACNNNNNCTCC 6 cut(s) 44, 74, 98, 128, 302, 332
Bsc4I CCNNNNNNNGG 3 cut(s) 452, 751, 1341
Bse1I ACTGG 4 cut(s) 526, 1132, 1472, 1551
Bse3DI GCAATG 6 cut(s) 493, 551, 1032, 1179, 1836, 1924
Bse8I GATNNNNATC 2 cut(s) 594, 1308
BseBI CCWGG 2 cut(s) 1527, 1770
BseDI CCNNGG 3 cut(s) 858, 1372, 1592
BseGI GGATG 4 cut(s) 803, 1555, 1582, 1588
BseJI GATNNNNATC 2 cut(s) 594, 1308
BseLI CCNNNNNNNGG 3 cut(s) 452, 751, 1341
BseMI GCAATG 6 cut(s) 493, 551, 1032, 1179, 1836, 1924
BseMII CTCAG 1 cut(s) 1854
BseNI ACTGG 4 cut(s) 526, 1132, 1472, 1551
BseRI GAGGAG 3 cut(s) 52, 200, 286
BseSI GKGCMC 1 cut(s) 40
BseXI GCAGC 4 cut(s) 819, 1517, 1545, 1706
Bsh1236I CGCG 2 cut(s) 952, 1067
BshFI GGCC 1 cut(s) 1790
BsiSI CCGG 1 cut(s) 1035
BslFI GGGAC 5 cut(s) 4, 710, 767, 1439, 1917
BslI CCNNNNNNNGG 3 cut(s) 452, 751, 1341
BsmAI GTCTC 4 cut(s) 77, 465, 1056, 1866
BsmBI CGTCTC 2 cut(s) 77, 1056
BsmFI GGGAC 5 cut(s) 4, 710, 767, 1439, 1917
BsmI GAATGC 2 cut(s) 999, 1620
BsnI GGCC 1 cut(s) 1790
Bso31I GGTCTC 1 cut(s) 1866
Bsp119I TTCGAA 1 cut(s) 1319
Bsp1286I GDGCHC 1 cut(s) 40
Bsp143I GATC 6 cut(s) 9, 1246, 1273, 1390, 1398, 1626
BspACI CCGC 9 cut(s) 338, 364, 689, 781, 786, 952, 997, 1067, 1876
BspANI GGCC 1 cut(s) 1790
BspCNI CTCAG 1 cut(s) 1853
BspFNI CGCG 2 cut(s) 952, 1067
BspHI TCATGA 1 cut(s) 658
BspMAI CTGCAG 1 cut(s) 1535
BspPI GGATC 1 cut(s) 1398
BspT104I TTCGAA 1 cut(s) 1319
BspTNI GGTCTC 1 cut(s) 1866
BsrBI CCGCTC 1 cut(s) 786
BsrDI GCAATG 6 cut(s) 493, 551, 1032, 1179, 1836, 1924
BsrI ACTGG 4 cut(s) 526, 1132, 1472, 1551
BssECI CCNNGG 3 cut(s) 858, 1372, 1592
BssMI GATC 6 cut(s) 9, 1246, 1273, 1390, 1398, 1626
BssSI CACGAG 2 cut(s) 433, 1562
BssT1I CCWWGG 3 cut(s) 858, 1372, 1592
Bst2BI CACGAG 2 cut(s) 433, 1562
Bst2UI CCWGG 2 cut(s) 1527, 1770
Bst4CI ACNGT 4 cut(s) 18, 35, 703, 1558
Bst6I CTCTTC 1 cut(s) 101
BstBI TTCGAA 1 cut(s) 1319
BstC8I GCNNGC 5 cut(s) 837, 1041, 1144, 1348, 1758
BstDEI CTNAG 2 cut(s) 373, 1840
BstF5I GGATG 4 cut(s) 803, 1555, 1582, 1588
BstFNI CGCG 2 cut(s) 952, 1067
BstH2I RGCGCY 1 cut(s) 915
BstHHI GCGC 2 cut(s) 914, 1191
BstKTI GATC 6 cut(s) 12, 1249, 1276, 1393, 1401, 1629
BstMAI GTCTC 4 cut(s) 77, 465, 1056, 1866
BstMBI GATC 6 cut(s) 9, 1246, 1273, 1390, 1398, 1626
BstMWI GCNNNNNNNGC 3 cut(s) 337, 1073, 1418
BstNI CCWGG 2 cut(s) 1527, 1770
BstNSI RCATGY 1 cut(s) 669
BstPAI GACNNNNGTC 1 cut(s) 1878
BstSCI CCNGG 3 cut(s) 1034, 1525, 1768
BstSFI CTRYAG 2 cut(s) 1455, 1531
BstSLI GKGCMC 1 cut(s) 40
BstUI CGCG 2 cut(s) 952, 1067
BstV1I GCAGC 4 cut(s) 819, 1517, 1545, 1706
BstXI CCANNNNNNTGG 1 cut(s) 1232
BsuRI GGCC 1 cut(s) 1790
BtsCI GGATG 4 cut(s) 803, 1555, 1582, 1588
BtsI GCAGTG 1 cut(s) 1050
BtsIMutI CAGTG 3 cut(s) 533, 699, 1050
Cac8I GCNNGC 5 cut(s) 837, 1041, 1144, 1348, 1758
CaiI CAGNNNCTG 1 cut(s) 22
CciI TCATGA 1 cut(s) 658
CfoI GCGC 2 cut(s) 914, 1191
Cfr13I GGNCC 1 cut(s) 1332
CseI GACGC 2 cut(s) 764, 1073
Csp6I GTAC 2 cut(s) 637, 886
CspCI CAANNNNNGTGG 2 cut(s) 1767, 1802
CviQI GTAC 2 cut(s) 637, 886
DdeI CTNAG 2 cut(s) 373, 1840
DpnI GATC 6 cut(s) 11, 1248, 1275, 1392, 1400, 1628
DpnII GATC 6 cut(s) 9, 1246, 1273, 1390, 1398, 1626
EaeI YGGCCR 1 cut(s) 1788
Eam1104I CTCTTC 1 cut(s) 101
EarI CTCTTC 1 cut(s) 101
Eco130I CCWWGG 3 cut(s) 858, 1372, 1592
Eco31I GGTCTC 1 cut(s) 1866
Eco47I GGWCC 1 cut(s) 1332
Eco57I CTGAAG 2 cut(s) 460, 1661
EcoO109I RGGNCCY 1 cut(s) 1332
EcoRI GAATTC 1 cut(s) 561
EcoRII CCWGG 2 cut(s) 1525, 1768
EcoT14I CCWWGG 3 cut(s) 858, 1372, 1592
EcoT22I ATGCAT 1 cut(s) 667
ErhI CCWWGG 3 cut(s) 858, 1372, 1592
Esp3I CGTCTC 2 cut(s) 77, 1056
FaqI GGGAC 5 cut(s) 4, 710, 767, 1439, 1917
FauI CCCGC 3 cut(s) 345, 371, 990
FbaI TGATCA 1 cut(s) 1273
FblI GTMKAC 1 cut(s) 1267
Fnu4HI GCNGC 4 cut(s) 833, 1531, 1534, 1695
FokI GGATG 4 cut(s) 810, 1562, 1575, 1589
Fsp4HI GCNGC 4 cut(s) 833, 1531, 1534, 1695
FspBI CTAG 3 cut(s) 576, 1619, 1736
GlaI GCGC 2 cut(s) 913, 1190
GluI GCNGC 4 cut(s) 833, 1531, 1534, 1695
HaeII RGCGCY 1 cut(s) 915
HaeIII GGCC 1 cut(s) 1790
HapII CCGG 1 cut(s) 1035
HgaI GACGC 2 cut(s) 764, 1073
HhaI GCGC 2 cut(s) 914, 1191
Hin6I GCGC 2 cut(s) 912, 1189
HinP1I GCGC 2 cut(s) 912, 1189
HincII GTYRAC 2 cut(s) 1268, 1479
HindII GTYRAC 2 cut(s) 1268, 1479
HindIII AAGCTT 2 cut(s) 837, 1278
HinfI GANTC 7 cut(s) 203, 293, 440, 1316, 1354, 1795, 1834
HpaI GTTAAC 1 cut(s) 1479
HpaII CCGG 1 cut(s) 1035
HphI GGTGA 3 cut(s) 67, 91, 435
Hpy166II GTNNAC 4 cut(s) 824, 1268, 1479, 1561
Hpy188I TCNGA 4 cut(s) 479, 604, 627, 1675
Hpy188III TCNNGA 5 cut(s) 290, 659, 1313, 1415, 1898
Hpy8I GTNNAC 4 cut(s) 824, 1268, 1479, 1561
HpyAV CCTTC 5 cut(s) 94, 523, 1495, 1591, 1696
HpyCH4III ACNGT 4 cut(s) 18, 35, 703, 1558
HpyCH4IV ACGT 3 cut(s) 774, 1381, 1906
HpyF10VI GCNNNNNNNGC 3 cut(s) 337, 1073, 1418
HpyF3I CTNAG 2 cut(s) 373, 1840
HpySE526I ACGT 3 cut(s) 774, 1381, 1906
HspAI GCGC 2 cut(s) 912, 1189
Ksp22I TGATCA 1 cut(s) 1273
KspAI GTTAAC 1 cut(s) 1479
Kzo9I GATC 6 cut(s) 9, 1246, 1273, 1390, 1398, 1626
LmnI GCTCC 1 cut(s) 783
Lsp1109I GCAGC 4 cut(s) 819, 1517, 1545, 1706
LweI GCATC 3 cut(s) 604, 652, 1077
MaeI CTAG 3 cut(s) 576, 1619, 1736
MaeII ACGT 3 cut(s) 774, 1381, 1906
MaeIII GTNAC 1 cut(s) 814
MalI GATC 6 cut(s) 11, 1248, 1275, 1392, 1400, 1628
MbiI CCGCTC 1 cut(s) 786
MboI GATC 6 cut(s) 9, 1246, 1273, 1390, 1398, 1626
MboII GAAGA 4 cut(s) 88, 508, 1654, 1790
MhlI GDGCHC 1 cut(s) 40
MlsI TGGCCA 1 cut(s) 1790
MluCI AATT 7 cut(s) 561, 684, 1484, 1512, 1684, 1779, 1863
MluNI TGGCCA 1 cut(s) 1790
MlyI GAGTC 2 cut(s) 197, 287
MmeI TCCRAC 1 cut(s) 70
Mox20I TGGCCA 1 cut(s) 1790
Mph1103I ATGCAT 1 cut(s) 667
MroXI GAANNNNTTC 1 cut(s) 876
MscI TGGCCA 1 cut(s) 1790
MseI TTAA 5 cut(s) 353, 537, 987, 1308, 1478
MslI CAYNNNNRTG 3 cut(s) 903, 1230, 1362
Msp20I TGGCCA 1 cut(s) 1790
MspA1I CMGCKG 1 cut(s) 1639
MspI CCGG 1 cut(s) 1035
MspR9I CCNGG 3 cut(s) 1036, 1527, 1770
Mva1269I GAATGC 2 cut(s) 999, 1620
MvaI CCWGG 2 cut(s) 1527, 1770
MvnI CGCG 2 cut(s) 952, 1067
MwoI GCNNNNNNNGC 3 cut(s) 337, 1073, 1418
NciI CCSGG 1 cut(s) 1036
NdeII GATC 6 cut(s) 9, 1246, 1273, 1390, 1398, 1626
NsiI ATGCAT 1 cut(s) 667
NspI RCATGY 1 cut(s) 669
NspV TTCGAA 1 cut(s) 1319
PagI TCATGA 1 cut(s) 658
PcsI WCGNNNNNNNCGW 2 cut(s) 1264, 1903
PctI GAATGC 2 cut(s) 999, 1620
PdmI GAANNNNTTC 1 cut(s) 876
PfeI GAWTC 5 cut(s) 440, 1316, 1354, 1795, 1834
PkrI GCNGC 4 cut(s) 834, 1532, 1535, 1696
PleI GAGTC 2 cut(s) 197, 287
PpsI GAGTC 2 cut(s) 197, 287
PpuMI RGGWCCY 1 cut(s) 1332
PshAI GACNNNNGTC 1 cut(s) 1878
PshBI ATTAAT 1 cut(s) 1308
PsiI TTATAA 2 cut(s) 1292, 1851
Psp5II RGGWCCY 1 cut(s) 1332
Psp6I CCWGG 2 cut(s) 1525, 1768
PspGI CCWGG 2 cut(s) 1525, 1768
PspPI GGNCC 1 cut(s) 1332
PspPPI RGGWCCY 1 cut(s) 1332
PstI CTGCAG 1 cut(s) 1535
PstNI CAGNNNCTG 1 cut(s) 22
PvuII CAGCTG 1 cut(s) 1639
RsaI GTAC 2 cut(s) 638, 887
RsaNI GTAC 2 cut(s) 637, 886
RseI CAYNNNNRTG 3 cut(s) 903, 1230, 1362
SalI GTCGAC 1 cut(s) 1266
SaqAI TTAA 5 cut(s) 353, 537, 987, 1308, 1478
SatI GCNGC 4 cut(s) 833, 1531, 1534, 1695
Sau3AI GATC 6 cut(s) 9, 1246, 1273, 1390, 1398, 1626
Sau96I GGNCC 1 cut(s) 1332
SchI GAGTC 2 cut(s) 197, 287
ScrFI CCNGG 3 cut(s) 1036, 1527, 1770
SduI GDGCHC 1 cut(s) 40
SfaNI GCATC 3 cut(s) 604, 652, 1077
SfcI CTRYAG 2 cut(s) 1455, 1531
SfuI TTCGAA 1 cut(s) 1319
SinI GGWCC 1 cut(s) 1332
SmiMI CAYNNNNRTG 3 cut(s) 903, 1230, 1362
SmlI CTYRAG 2 cut(s) 1406, 1413
SmoI CTYRAG 2 cut(s) 1406, 1413
Sse9I AATT 7 cut(s) 561, 684, 1484, 1512, 1684, 1779, 1863
SsiI CCGC 9 cut(s) 338, 364, 689, 781, 786, 952, 997, 1067, 1876
SspI AATATT 1 cut(s) 1447
SspMI CTAG 3 cut(s) 576, 1619, 1736
StyD4I CCNGG 3 cut(s) 1034, 1525, 1768
StyI CCWWGG 3 cut(s) 858, 1372, 1592
TaaI ACNGT 4 cut(s) 18, 35, 703, 1558
TaiI ACGT 3 cut(s) 777, 1384, 1909
TaqI TCGA 8 cut(s) 120, 291, 641, 678, 1267, 1298, 1319, 1401
TasI AATT 7 cut(s) 561, 684, 1484, 1512, 1684, 1779, 1863
TatI WGTACW 1 cut(s) 636
TfiI GAWTC 5 cut(s) 440, 1316, 1354, 1795, 1834
Tru1I TTAA 5 cut(s) 353, 537, 987, 1308, 1478
Tru9I TTAA 5 cut(s) 353, 537, 987, 1308, 1478
TscAI CASTG 3 cut(s) 533, 706, 1050
TseI GCWGC 4 cut(s) 832, 1530, 1533, 1694
TspGWI ACGGA 1 cut(s) 810
TspRI CASTG 3 cut(s) 533, 706, 1050
VpaK11BI GGWCC 1 cut(s) 1332
VspI ATTAAT 1 cut(s) 1308
XapI RAATTY 2 cut(s) 561, 684
XceI RCATGY 1 cut(s) 669
XmiI GTMKAC 1 cut(s) 1267
XmnI GAANNNNTTC 1 cut(s) 876
XspI CTAG 3 cut(s) 576, 1619, 1736
Zsp2I ATGCAT 1 cut(s) 667
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.