MD14G1200800.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0016162
Physical Location & Seq
Forward (+)
29002913 .. 29004001
1089 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1200800.v1.1.491

Sequence Viewer

Length: 894 bp
ATGATAAGAGCTTACTCCAAGAGCGACTCGCCGGCGGAGGGGTTTTATCTGTTCAGATATTTGCGGCGGCGCGGCCTTTTAGCTGGCTCTATGGCGTCGTCTTTCGTCATCAAGTCTTGCATTAGAGTTTCGTCGTTGAGAGATACTGTGGCCTTGGGTTTGTTTGAGATTATGCAGAGTGAGGTTCATAGGTGTGAGCCTGATGACGTTACGTGTTTACTTGTGCTCCAAGCGTGTGCCAGCTTGAACGCGTTGGAGTTTGGCAAACGGGTTCATAAGTACATCGAAGAACATGGTTATGGTGGTGCTTCAAATTTGTATGACTCTCTTGTATTGATGTATTCACGGTGTTGGAGTTTGGATAAGGCGTATGGGGTGTTTGAGGGAATGCAATATAAGAATGTGCTATTGAAGCGTTTGGAGAGATGCAGAAAATGGGTGTTTTACCTGATGATCAGACTTTTACTGGGTCTTTGTGCTTGCAGCCATTGCGGTTTGGTTGATGAAAGGATGATGATTTTCGATCGTATGAGCAAAGAGTTTGGGGTAGTGCCTAATATCCATCACTATGGGCGCATGTTTGATCTTTTGGGTCGTGCTGGTCGACTTGATCAGGCCTATCAGCTCATAACATCGATGAACATGAAACCAGATCCGACAATATGGAGGACCTTACTTGGGGCTTGCAGAATTCACGGCAATAATTCCCTTGCGGAACGAGTGGTAGGCAATTTGATTGAACTCAAGGCTCAAGAGGCAGGGGATTATGTCCTGTTGATGAATATTTTCTCTTCAGCTGGAACCTGGGAGAAGCTAACAGAAATGAGGAAGTTTATGAAGGAAAAAGCAATTCAAACTACACCGGGATGTAGCACGCTTGAACTAAAGGAGTAG

Protein Analysis

298

Amino Acids

33.91

Weight (kDa)

8.9

Isoelectric Point (pI)

43.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
E_motif PF20431 232 - 294 1.4e-14 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016162)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G47530
fragaria_vesca FvH4_5g14900
malus_domestica MD06G1193700.v1.1 MD14G1200800.v1.1
prunus_persica Prupe.5G194000_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0181481
rosa_laevigata RLG00000005190
rosa_multiflora Rmu_co8291233.1_g000001 Rmu_sc0002060.1_g000023
rosa_roxburghii Rroxscaffold_3G00271960
rosa_rugosa Rorug06G0441700
rosa_samantha Rh7AG043500 Rh7BG042900 Rh7CG044700
rosa_wichuraiana Rw7G003620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 604
AccII CGCG 2 cut(s) 72, 251
AciI CCGC 6 cut(s) 35, 64, 67, 72, 492, 713
AclWI GGATC 1 cut(s) 647
AcsI RAATTY 2 cut(s) 313, 690
AcuI CTGAAG 1 cut(s) 777
AcyI GRCGYC 1 cut(s) 95
AfaI GTAC 1 cut(s) 281
AfiI CCNNNNNNNGG 2 cut(s) 38, 678
AflIII ACRYGT 2 cut(s) 212, 249
AgsI TTSAA 6 cut(s) 247, 312, 412, 740, 854, 881
AjnI CCWGG 1 cut(s) 803
AluBI AGCT 6 cut(s) 11, 83, 243, 625, 797, 814
AluI AGCT 6 cut(s) 11, 83, 243, 625, 797, 814
Alw21I GWGCWC 1 cut(s) 228
AlwI GGATC 1 cut(s) 647
AoxI GGCC 3 cut(s) 73, 150, 615
ApeKI GCWGC 1 cut(s) 483
ApoI RAATTY 2 cut(s) 313, 690
Asp700I GAANNNNTTC 1 cut(s) 785
AspLEI GCGC 2 cut(s) 72, 576
AspS9I GGNCC 1 cut(s) 669
AsuC2I CCSGG 1 cut(s) 864
AvaII GGWCC 1 cut(s) 669
Bbv12I GWGCWC 1 cut(s) 228
BbvI GCAGC 1 cut(s) 495
BccI CCATC 1 cut(s) 570
BceAI ACGGC 1 cut(s) 712
BciT130I CCWGG 1 cut(s) 805
BclI TGATCA 2 cut(s) 453, 610
BcnI CCSGG 1 cut(s) 864
BisI GCNGC 4 cut(s) 65, 68, 73, 484
BlsI GCNGC 4 cut(s) 66, 69, 74, 485
Bme1390I CCNGG 2 cut(s) 805, 864
Bme18I GGWCC 1 cut(s) 669
BmgT120I GGNCC 1 cut(s) 669
BmiI GGNNCC 1 cut(s) 802
BmrFI CCNGG 2 cut(s) 805, 864
BmrI ACTGGG 1 cut(s) 476
BmsI GCATC 1 cut(s) 416
BmuI ACTGGG 1 cut(s) 476
BpuEI CTTGAG 2 cut(s) 728, 735
BpuMI CCSGG 1 cut(s) 864
Bsa29I ATCGAT 1 cut(s) 635
BsaAI YACGTR 1 cut(s) 213
BsaHI GRCGYC 1 cut(s) 95
BsaJI CCNNGG 2 cut(s) 153, 804
BsaXI ACNNNNNCTCC 2 cut(s) 210, 240
Bsc4I CCNNNNNNNGG 2 cut(s) 38, 678
Bse118I RCCGGY 1 cut(s) 31
Bse1I ACTGG 1 cut(s) 471
Bse3DI GCAATG 1 cut(s) 487
BseBI CCWGG 1 cut(s) 805
BseCI ATCGAT 1 cut(s) 635
BseDI CCNNGG 2 cut(s) 153, 804
BseGI GGATG 2 cut(s) 516, 872
BseLI CCNNNNNNNGG 2 cut(s) 38, 678
BseMI GCAATG 1 cut(s) 487
BseNI ACTGG 1 cut(s) 471
BseXI GCAGC 1 cut(s) 495
Bsh1236I CGCG 2 cut(s) 72, 251
Bsh1285I CGRYCG 1 cut(s) 526
BshFI GGCC 3 cut(s) 75, 152, 617
BshVI ATCGAT 1 cut(s) 635
BsiEI CGRYCG 1 cut(s) 526
BsiHKAI GWGCWC 1 cut(s) 228
BsiSI CCGG 2 cut(s) 32, 863
BslI CCNNNNNNNGG 2 cut(s) 38, 678
BsmI GAATGC 1 cut(s) 393
BsnI GGCC 3 cut(s) 75, 152, 617
Bsp1286I GDGCHC 1 cut(s) 228
Bsp143I GATC 5 cut(s) 453, 523, 583, 610, 652
BspACI CCGC 6 cut(s) 35, 64, 67, 72, 492, 713
BspANI GGCC 3 cut(s) 75, 152, 617
BspDI ATCGAT 1 cut(s) 635
BspFNI CGCG 2 cut(s) 72, 251
BspLI GGNNCC 1 cut(s) 802
BspPI GGATC 1 cut(s) 647
BsrDI GCAATG 1 cut(s) 487
BsrFI RCCGGY 1 cut(s) 31
BsrI ACTGG 1 cut(s) 471
BssAI RCCGGY 1 cut(s) 31
BssECI CCNNGG 2 cut(s) 153, 804
BssMI GATC 5 cut(s) 453, 523, 583, 610, 652
BssNI GRCGYC 1 cut(s) 95
BssT1I CCWWGG 1 cut(s) 153
Bst2UI CCWGG 1 cut(s) 805
Bst4CI ACNGT 2 cut(s) 148, 348
Bst6I CTCTTC 1 cut(s) 796
BstACI GRCGYC 1 cut(s) 95
BstAPI GCANNNNNTGC 1 cut(s) 489
BstBAI YACGTR 1 cut(s) 213
BstC8I GCNNGC 6 cut(s) 33, 85, 241, 481, 685, 875
BstF5I GGATG 2 cut(s) 516, 872
BstFNI CGCG 2 cut(s) 72, 251
BstHHI GCGC 2 cut(s) 72, 576
BstKTI GATC 5 cut(s) 456, 526, 586, 613, 655
BstMBI GATC 5 cut(s) 453, 523, 583, 610, 652
BstMCI CGRYCG 1 cut(s) 526
BstMWI GCNNNNNNNGC 3 cut(s) 412, 489, 755
BstNI CCWGG 1 cut(s) 805
BstNSI RCATGY 1 cut(s) 580
BstSCI CCNGG 2 cut(s) 803, 862
BstUI CGCG 2 cut(s) 72, 251
BstV1I GCAGC 1 cut(s) 495
BstX2I RGATCY 1 cut(s) 652
BstXI CCANNNNNNTGG 1 cut(s) 569
BstYI RGATCY 1 cut(s) 652
Bsu15I ATCGAT 1 cut(s) 635
BsuRI GGCC 3 cut(s) 75, 152, 617
BsuTUI ATCGAT 1 cut(s) 635
BtsCI GGATG 2 cut(s) 516, 872
Cac8I GCNNGC 6 cut(s) 33, 85, 241, 481, 685, 875
CfoI GCGC 2 cut(s) 72, 576
Cfr10I RCCGGY 1 cut(s) 31
Cfr13I GGNCC 1 cut(s) 669
ClaI ATCGAT 1 cut(s) 635
CseI GACGC 1 cut(s) 84
Csp6I GTAC 1 cut(s) 280
CviAII CATG 3 cut(s) 293, 577, 643
CviQI GTAC 1 cut(s) 280
DpnI GATC 5 cut(s) 455, 525, 585, 612, 654
DpnII GATC 5 cut(s) 453, 523, 583, 610, 652
Eam1104I CTCTTC 1 cut(s) 796
EarI CTCTTC 1 cut(s) 796
EciI GGCGGA 1 cut(s) 50
Eco130I CCWWGG 1 cut(s) 153
Eco147I AGGCCT 1 cut(s) 617
Eco47I GGWCC 1 cut(s) 669
Eco57I CTGAAG 1 cut(s) 777
EcoO109I RGGNCCY 1 cut(s) 669
EcoRI GAATTC 1 cut(s) 690
EcoRII CCWGG 1 cut(s) 803
EcoT14I CCWWGG 1 cut(s) 153
ErhI CCWWGG 1 cut(s) 153
FaeI CATG 3 cut(s) 296, 580, 646
FatI CATG 3 cut(s) 292, 576, 642
FbaI TGATCA 2 cut(s) 453, 610
FblI GTMKAC 1 cut(s) 604
Fnu4HI GCNGC 4 cut(s) 65, 68, 73, 484
FokI GGATG 2 cut(s) 523, 879
Fsp4HI GCNGC 4 cut(s) 65, 68, 73, 484
GlaI GCGC 2 cut(s) 71, 575
GluI GCNGC 4 cut(s) 65, 68, 73, 484
HaeIII GGCC 3 cut(s) 75, 152, 617
HapII CCGG 2 cut(s) 32, 863
HgaI GACGC 1 cut(s) 84
HhaI GCGC 2 cut(s) 72, 576
Hin1I GRCGYC 1 cut(s) 95
Hin1II CATG 3 cut(s) 296, 580, 646
Hin6I GCGC 2 cut(s) 70, 574
HinP1I GCGC 2 cut(s) 70, 574
HincII GTYRAC 1 cut(s) 605
HindII GTYRAC 1 cut(s) 605
HinfI GANTC 2 cut(s) 26, 323
HpaII CCGG 2 cut(s) 32, 863
Hpy166II GTNNAC 2 cut(s) 218, 605
Hpy188I TCNGA 3 cut(s) 56, 458, 657
Hpy188III TCNNGA 1 cut(s) 752
Hpy8I GTNNAC 2 cut(s) 218, 605
Hpy99I CGWCG 2 cut(s) 100, 136
HpyAV CCTTC 1 cut(s) 832
HpyCH4III ACNGT 2 cut(s) 148, 348
HpyCH4IV ACGT 2 cut(s) 207, 212
HpyCH4V TGCA 6 cut(s) 120, 175, 391, 429, 483, 687
HpyF10VI GCNNNNNNNGC 3 cut(s) 412, 489, 755
HpySE526I ACGT 2 cut(s) 207, 212
Hsp92I GRCGYC 1 cut(s) 95
Hsp92II CATG 3 cut(s) 296, 580, 646
HspAI GCGC 2 cut(s) 70, 574
KroI GCCGGC 1 cut(s) 31
KroNI GCCGGC 1 cut(s) 33
Ksp22I TGATCA 2 cut(s) 453, 610
Kzo9I GATC 5 cut(s) 453, 523, 583, 610, 652
LmnI GCTCC 1 cut(s) 231
Lsp1109I GCAGC 1 cut(s) 495
LweI GCATC 1 cut(s) 416
MaeII ACGT 2 cut(s) 207, 212
MaeIII GTNAC 1 cut(s) 208
MalI GATC 5 cut(s) 455, 525, 585, 612, 654
MboI GATC 5 cut(s) 453, 523, 583, 610, 652
MboII GAAGA 2 cut(s) 299, 783
MflI RGATCY 1 cut(s) 652
MhlI GDGCHC 1 cut(s) 228
MluCI AATT 5 cut(s) 313, 690, 703, 730, 849
MluI ACGCGT 1 cut(s) 249
MlyI GAGTC 2 cut(s) 20, 317
MmeI TCCRAC 3 cut(s) 234, 332, 680
MnlI CCTC 6 cut(s) 31, 175, 376, 660, 748, 819
MreI CGCCGGCG 1 cut(s) 31
MroNI GCCGGC 1 cut(s) 31
MroXI GAANNNNTTC 1 cut(s) 785
MslI CAYNNNNRTG 4 cut(s) 192, 297, 567, 865
MspA1I CMGCKG 1 cut(s) 797
MspI CCGG 2 cut(s) 32, 863
MspR9I CCNGG 2 cut(s) 805, 864
Mva1269I GAATGC 1 cut(s) 393
MvaI CCWGG 1 cut(s) 805
MvnI CGCG 2 cut(s) 72, 251
MwoI GCNNNNNNNGC 3 cut(s) 412, 489, 755
NaeI GCCGGC 1 cut(s) 33
NciI CCSGG 1 cut(s) 864
NdeII GATC 5 cut(s) 453, 523, 583, 610, 652
NgoMIV GCCGGC 1 cut(s) 31
NlaIII CATG 3 cut(s) 296, 580, 646
NlaIV GGNNCC 1 cut(s) 802
NspI RCATGY 1 cut(s) 580
PceI AGGCCT 1 cut(s) 617
PcsI WCGNNNNNNNCGW 1 cut(s) 601
PctI GAATGC 1 cut(s) 393
PdiI GCCGGC 1 cut(s) 33
PdmI GAANNNNTTC 1 cut(s) 785
PkrI GCNGC 4 cut(s) 66, 69, 74, 485
Ple19I CGATCG 1 cut(s) 526
PleI GAGTC 2 cut(s) 20, 317
PpsI GAGTC 2 cut(s) 20, 317
Ppu21I YACGTR 1 cut(s) 213
PpuMI RGGWCCY 1 cut(s) 669
Psp5II RGGWCCY 1 cut(s) 669
Psp6I CCWGG 1 cut(s) 803
PspGI CCWGG 1 cut(s) 803
PspN4I GGNNCC 1 cut(s) 802
PspPI GGNCC 1 cut(s) 669
PspPPI RGGWCCY 1 cut(s) 669
PsuI RGATCY 1 cut(s) 652
PvuI CGATCG 1 cut(s) 526
PvuII CAGCTG 1 cut(s) 797
RsaI GTAC 1 cut(s) 281
RsaNI GTAC 1 cut(s) 280
RseI CAYNNNNRTG 4 cut(s) 192, 297, 567, 865
SalI GTCGAC 1 cut(s) 603
SatI GCNGC 4 cut(s) 65, 68, 73, 484
Sau3AI GATC 5 cut(s) 453, 523, 583, 610, 652
Sau96I GGNCC 1 cut(s) 669
SchI GAGTC 2 cut(s) 20, 317
ScrFI CCNGG 2 cut(s) 805, 864
SduI GDGCHC 1 cut(s) 228
SfaNI GCATC 1 cut(s) 416
SgrAI CRCCGGYG 1 cut(s) 31
SinI GGWCC 1 cut(s) 669
SmiMI CAYNNNNRTG 4 cut(s) 192, 297, 567, 865
SmlI CTYRAG 2 cut(s) 743, 750
SmoI CTYRAG 2 cut(s) 743, 750
Sse9I AATT 5 cut(s) 313, 690, 703, 730, 849
SseBI AGGCCT 1 cut(s) 617
SsiI CCGC 6 cut(s) 35, 64, 67, 72, 492, 713
SspI AATATT 1 cut(s) 784
StuI AGGCCT 1 cut(s) 617
StyD4I CCNGG 2 cut(s) 803, 862
StyI CCWWGG 1 cut(s) 153
TaaI ACNGT 2 cut(s) 148, 348
TaiI ACGT 2 cut(s) 210, 215
TaqI TCGA 4 cut(s) 285, 522, 604, 635
TasI AATT 5 cut(s) 313, 690, 703, 730, 849
TatI WGTACW 1 cut(s) 279
TauI GCSGC 3 cut(s) 67, 70, 75
TseI GCWGC 1 cut(s) 483
TspDTI ATGAA 7 cut(s) 176, 263, 519, 653, 659, 794, 851
VpaK11BI GGWCC 1 cut(s) 669
XapI RAATTY 2 cut(s) 313, 690
XceI RCATGY 1 cut(s) 580
XmiI GTMKAC 1 cut(s) 604
XmnI GAANNNNTTC 1 cut(s) 785
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.