Rh7CG044700

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0016162
Physical Location & Seq
Forward (+)
3006918 .. 3008910
1993 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG044700.1

Sequence Viewer

Length: 1554 bp
ATGACCGCAGTCCCACACCCCCTGCGCTCAAGCTGTGGCCGTCTCAAAGACCACAACATCTCTTGTACTCGTTTCACCTCCACCCTCTCATCACTCGTCACCTTCAACTCTCCAAAACCCCATCACCGCCAAAACCAGACCAAACCCATCATCCCCTATACTCAAACCCTCAAAGACTCCCTCCTCTCCCTCATCAAATCATGCACCCACAAATCCCATTTGCTCCAAATCCACGCCCACATTCTCCAAACCTCTCTCATTCTCGACTCCTCAATCTGCTTCCACTTCTTGTCCCTCGTTTCGCTTTCCCCACCTTTCCAAAACTTGACCTATTCCCGCCGATTCTTGTTTCAAATTCCCAAACCCAAAGCCATTCACTACAACACTGTGATAAGAGCTTACTCAATGAGTGATTCACCCCAGGAGGGGATTCATCTTTACCTTGATTTGAGGCGGCGAGGTCTTGGTAGCAACGCTCTCTCGTCCTCTTTCGTAATGAAATGTTGTGTTAGAGCCAAGTACTTATTAGGAGGAATTCAGGTTCAGGCTAGGATTGTGAGAGATGGGCAGCAATCAGACAGTCGTTTACTCACGACTTTGATGGACCTGTACTCGATTTGTGGCGAGTTTGATGATGCATGTAAGGTGTTCGACGAAATTCTTCACAGAGACACTGTTGCCTGGAATGTGTTGATTTCTTGTTTTCTGCATAACAGCCGCAGCAGGGATGCTTTGGGTTTGTTTGACATTATGCGGAGCGAAAGTTACAGATGTGAGCCTGATGATGTTACTTGTTTACTTATGCTCCAAGCTTGTGCAAGCTTGAATGCGTTGGAGTTTGGCCAAAGGGTTCATAGGTACATTGAAGAACATGCTTATATTGGTGCTTCTAATCTCTGTAATGCTCTTATAACTATGTATTCGCGGTGTGGGTGTTTGGATCAGGCTTATGAGGTGTTTAAGGGGATGCGGGGTCGAAATGTGGTTTCATGGAGTGCAATCATTTCTGGGCTTGCTGTGAACGGGCATGGCAGAGACGCTGTTGAAGCGTTTTGTGAGATGCAGAGAATGGGGGTTTTACCTGATGAACAGACTTTTACTGGCGTTCTTTCGGCTTGCAGTCATTGTGGGTTGGTTGATGAAGCAATGGATATTTTTGATCGCATGAGCAAAGAGTTTGGAGTAGTGCCTAATGTCCATCACTATGGGTGCTTGGTTGATCTTTTGGGTCGGGCTGGTCGACTCGATCAAGCATATCAGCTTATAATGTCGATGGATATGAAACCAGATTCAAAGATATGGAGAACCTTACTTGGGGCTTGCAAGATTCATAACTATGAAACCCTTGGGGAACGTGCGGCGGATCATTTGATCGAACTCAAGGCTCTAGAGGCAGGGGACTATGTTCTGTTGATGAATATTTACTCTTCAGCTGAAAACTGGGAGAAATTAGCTGAATTGAGCAAGAACATTCAAGAACTACGGCATGTGAGCATCAGGGAACATGTTCTGCAAATGAAATGGCTGGGTGGGAAGCAACACAGAGTTTTATAG

Protein Analysis

517

Amino Acids

58.45

Weight (kDa)

8.18

Isoelectric Point (pI)

50.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 223 - 273 1.2e-08 PPR repeat family
PPR_long PF17177 290 - 423 2.2e-07 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 326 - 374 8.2e-12 PPR repeat family
PPR PF01535 329 - 359 4.5e-06 PPR repeat
PPR_1 PF12854 394 - 424 1e-05 PPR repeat
E_motif PF20431 446 - 490 4.2e-07 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016162)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G47530
fragaria_vesca FvH4_5g14900
malus_domestica MD06G1193700.v1.1 MD14G1200800.v1.1
prunus_persica Prupe.5G194000_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0181481
rosa_laevigata RLG00000005190
rosa_multiflora Rmu_co8291233.1_g000001 Rmu_sc0002060.1_g000023
rosa_roxburghii Rroxscaffold_3G00271960
rosa_rugosa Rorug06G0441700
rosa_samantha Rh7AG043500 Rh7BG042900 Rh7CG044700
rosa_wichuraiana Rw7G003620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 911, 1265
AccI GTMKAC 1 cut(s) 1240
AccII CGCG 1 cut(s) 925
AclWI GGATC 2 cut(s) 948, 1371
AcoI YGGCCR 2 cut(s) 37, 841
AcsI RAATTY 3 cut(s) 354, 534, 657
AcuI CTGAAG 1 cut(s) 1413
AfaI GTAC 4 cut(s) 67, 521, 611, 860
AfiI CCNNNNNNNGG 4 cut(s) 425, 426, 724, 1314
AflIII ACRYGT 1 cut(s) 1504
AgsI TTSAA 7 cut(s) 106, 353, 826, 866, 1046, 1293, 1475
AjnI CCWGG 2 cut(s) 420, 680
AloI GAACNNNNNNTCC 2 cut(s) 525, 557
AluBI AGCT 7 cut(s) 33, 398, 812, 822, 1261, 1433, 1454
AluI AGCT 7 cut(s) 33, 398, 812, 822, 1261, 1433, 1454
Alw26I GTCTC 3 cut(s) 47, 663, 1029
AlwI GGATC 2 cut(s) 948, 1371
AoxI GGCC 2 cut(s) 37, 841
ApeKI GCWGC 2 cut(s) 568, 720
ApoI RAATTY 3 cut(s) 354, 534, 657
Asp700I GAANNNNTTC 2 cut(s) 660, 1506
AspLEI GCGC 1 cut(s) 27
AspS9I GGNCC 1 cut(s) 604
AsuHPI GGTGA 4 cut(s) 67, 91, 116, 408
AvaII GGWCC 1 cut(s) 604
BalI TGGCCA 1 cut(s) 843
BbvI GCAGC 2 cut(s) 580, 732
BccI CCATC 6 cut(s) 129, 155, 557, 595, 1206, 1267
BceAI ACGGC 2 cut(s) 24, 1499
BciT130I CCWGG 2 cut(s) 422, 682
BcoDI GTCTC 3 cut(s) 47, 663, 1029
BfaI CTAG 2 cut(s) 549, 1388
BisI GCNGC 5 cut(s) 455, 569, 718, 721, 1359
BlsI GCNGC 5 cut(s) 456, 570, 719, 722, 1360
BmcAI AGTACT 1 cut(s) 521
Bme1390I CCNGG 2 cut(s) 422, 682
Bme18I GGWCC 1 cut(s) 604
BmgT120I GGNCC 1 cut(s) 604
BmrFI CCNGG 2 cut(s) 422, 682
BmrI ACTGGG 1 cut(s) 1450
BmsI GCATC 5 cut(s) 625, 718, 957, 1050, 1503
BmuI ACTGGG 1 cut(s) 1450
BoxI GACNNNNGTC 1 cut(s) 8
BpuEI CTTGAG 2 cut(s) 13, 1364
BsaJI CCNNGG 2 cut(s) 420, 1345
BsaXI ACNNNNNCTCC 4 cut(s) 748, 778, 789, 819
Bsc4I CCNNNNNNNGG 4 cut(s) 425, 426, 724, 1314
Bse1I ACTGG 2 cut(s) 1105, 1445
Bse3DI GCAATG 1 cut(s) 1152
BseBI CCWGG 2 cut(s) 422, 682
BseDI CCNNGG 2 cut(s) 420, 1345
BseGI GGATG 3 cut(s) 150, 733, 972
BseLI CCNNNNNNNGG 4 cut(s) 425, 426, 724, 1314
BseMI GCAATG 1 cut(s) 1152
BseNI ACTGG 2 cut(s) 1105, 1445
BseRI GAGGAG 2 cut(s) 173, 259
BseXI GCAGC 2 cut(s) 580, 732
BseYI CCCAGC 1 cut(s) 1525
Bsh1236I CGCG 1 cut(s) 925
BshFI GGCC 2 cut(s) 39, 843
BslFI GGGAC 2 cut(s) 277, 1412
BslI CCNNNNNNNGG 4 cut(s) 425, 426, 724, 1314
BsmAI GTCTC 3 cut(s) 47, 663, 1029
BsmBI CGTCTC 2 cut(s) 47, 1029
BsmFI GGGAC 2 cut(s) 277, 1412
BsmI GAATGC 1 cut(s) 832
BsnI GGCC 2 cut(s) 39, 843
Bsp143I GATC 6 cut(s) 940, 1159, 1219, 1246, 1363, 1371
BspANI GGCC 2 cut(s) 39, 843
BspFNI CGCG 1 cut(s) 925
BspPI GGATC 2 cut(s) 948, 1371
BsrDI GCAATG 1 cut(s) 1152
BsrI ACTGG 2 cut(s) 1105, 1445
BssECI CCNNGG 2 cut(s) 420, 1345
BssMI GATC 6 cut(s) 940, 1159, 1219, 1246, 1363, 1371
BssT1I CCWWGG 1 cut(s) 1345
Bst2UI CCWGG 2 cut(s) 422, 682
Bst4CI ACNGT 3 cut(s) 388, 581, 676
Bst6I CTCTTC 1 cut(s) 1432
BstC8I GCNNGC 4 cut(s) 820, 1014, 1117, 1321
BstF5I GGATG 3 cut(s) 150, 733, 972
BstFNI CGCG 1 cut(s) 925
BstHHI GCGC 1 cut(s) 27
BstKTI GATC 6 cut(s) 943, 1162, 1222, 1249, 1366, 1374
BstMAI GTCTC 3 cut(s) 47, 663, 1029
BstMBI GATC 6 cut(s) 940, 1159, 1219, 1246, 1363, 1371
BstMWI GCNNNNNNNGC 2 cut(s) 1046, 1391
BstNI CCWGG 2 cut(s) 422, 682
BstNSI RCATGY 4 cut(s) 642, 875, 1490, 1508
BstPAI GACNNNNGTC 1 cut(s) 8
BstSCI CCNGG 2 cut(s) 420, 680
BstUI CGCG 1 cut(s) 925
BstV1I GCAGC 2 cut(s) 580, 732
BstXI CCANNNNNNTGG 1 cut(s) 1205
BsuRI GGCC 2 cut(s) 39, 843
BtsCI GGATG 3 cut(s) 150, 733, 972
BtsIMutI CAGTG 2 cut(s) 384, 672
Cac8I GCNNGC 4 cut(s) 820, 1014, 1117, 1321
CfoI GCGC 1 cut(s) 27
Cfr13I GGNCC 1 cut(s) 604
CseI GACGC 1 cut(s) 1046
Csp6I GTAC 4 cut(s) 66, 520, 610, 859
CviAII CATG 8 cut(s) 201, 639, 872, 990, 1028, 1165, 1487, 1505
CviQI GTAC 4 cut(s) 66, 520, 610, 859
DpnI GATC 6 cut(s) 942, 1161, 1221, 1248, 1365, 1373
DpnII GATC 6 cut(s) 940, 1159, 1219, 1246, 1363, 1371
EaeI YGGCCR 2 cut(s) 37, 841
Eam1104I CTCTTC 1 cut(s) 1432
EarI CTCTTC 1 cut(s) 1432
EciI GGCGGA 1 cut(s) 1376
Eco130I CCWWGG 1 cut(s) 1345
Eco47I GGWCC 1 cut(s) 604
Eco57I CTGAAG 1 cut(s) 1413
EcoRI GAATTC 1 cut(s) 534
EcoRII CCWGG 2 cut(s) 420, 680
EcoT14I CCWWGG 1 cut(s) 1345
EcoT22I ATGCAT 1 cut(s) 640
ErhI CCWWGG 1 cut(s) 1345
Esp3I CGTCTC 2 cut(s) 47, 1029
FaeI CATG 8 cut(s) 204, 642, 875, 993, 1031, 1168, 1490, 1508
FaqI GGGAC 2 cut(s) 277, 1412
FatI CATG 8 cut(s) 200, 638, 871, 989, 1027, 1164, 1486, 1504
FauI CCCGC 2 cut(s) 344, 963
FblI GTMKAC 1 cut(s) 1240
Fnu4HI GCNGC 5 cut(s) 455, 569, 718, 721, 1359
FokI GGATG 3 cut(s) 137, 740, 979
Fsp4HI GCNGC 5 cut(s) 455, 569, 718, 721, 1359
FspBI CTAG 2 cut(s) 549, 1388
GlaI GCGC 1 cut(s) 26
GluI GCNGC 5 cut(s) 455, 569, 718, 721, 1359
GsaI CCCAGC 1 cut(s) 1529
HaeIII GGCC 2 cut(s) 39, 843
HgaI GACGC 1 cut(s) 1046
HhaI GCGC 1 cut(s) 27
Hin1II CATG 8 cut(s) 204, 642, 875, 993, 1031, 1168, 1490, 1508
Hin6I GCGC 1 cut(s) 25
HinP1I GCGC 1 cut(s) 25
HincII GTYRAC 1 cut(s) 1241
HindII GTYRAC 1 cut(s) 1241
HindIII AAGCTT 2 cut(s) 810, 820
HinfI GANTC 8 cut(s) 176, 266, 342, 413, 430, 1242, 1289, 1327
HphI GGTGA 4 cut(s) 67, 91, 116, 408
Hpy166II GTNNAC 4 cut(s) 587, 797, 1021, 1241
Hpy188I TCNGA 1 cut(s) 577
Hpy188III TCNNGA 4 cut(s) 263, 592, 1388, 1475
Hpy8I GTNNAC 4 cut(s) 587, 797, 1021, 1241
Hpy99I CGWCG 1 cut(s) 656
HpyAV CCTTC 1 cut(s) 112
HpyCH4III ACNGT 3 cut(s) 388, 581, 676
HpyCH4IV ACGT 1 cut(s) 1354
HpyCH4V TGCA 9 cut(s) 204, 638, 709, 818, 998, 1063, 1119, 1323, 1513
HpyF10VI GCNNNNNNNGC 2 cut(s) 1046, 1391
HpySE526I ACGT 1 cut(s) 1354
Hsp92II CATG 8 cut(s) 204, 642, 875, 993, 1031, 1168, 1490, 1508
HspAI GCGC 1 cut(s) 25
Kzo9I GATC 6 cut(s) 940, 1159, 1219, 1246, 1363, 1371
LmnI GCTCC 3 cut(s) 228, 756, 810
Lsp1109I GCAGC 2 cut(s) 580, 732
LweI GCATC 5 cut(s) 625, 718, 957, 1050, 1503
MaeI CTAG 2 cut(s) 549, 1388
MaeII ACGT 1 cut(s) 1354
MaeIII GTNAC 3 cut(s) 97, 764, 787
MalI GATC 6 cut(s) 942, 1161, 1221, 1248, 1365, 1373
MboI GATC 6 cut(s) 940, 1159, 1219, 1246, 1363, 1371
MboII GAAGA 3 cut(s) 653, 878, 1419
MlsI TGGCCA 1 cut(s) 843
MluCI AATT 5 cut(s) 354, 534, 657, 1448, 1457
MluNI TGGCCA 1 cut(s) 843
MlyI GAGTC 3 cut(s) 170, 260, 1236
MmeI TCCRAC 1 cut(s) 813
Mox20I TGGCCA 1 cut(s) 843
Mph1103I ATGCAT 1 cut(s) 640
MroXI GAANNNNTTC 2 cut(s) 660, 1506
MscI TGGCCA 1 cut(s) 843
MseI TTAA 1 cut(s) 960
MslI CAYNNNNRTG 2 cut(s) 1203, 1335
Msp20I TGGCCA 1 cut(s) 843
MspA1I CMGCKG 1 cut(s) 1433
MspR9I CCNGG 2 cut(s) 422, 682
Mva1269I GAATGC 1 cut(s) 832
MvaI CCWGG 2 cut(s) 422, 682
MvnI CGCG 1 cut(s) 925
MwoI GCNNNNNNNGC 2 cut(s) 1046, 1391
NdeII GATC 6 cut(s) 940, 1159, 1219, 1246, 1363, 1371
NlaIII CATG 8 cut(s) 204, 642, 875, 993, 1031, 1168, 1490, 1508
NmuCI GTSAC 1 cut(s) 97
NsiI ATGCAT 1 cut(s) 640
NspI RCATGY 4 cut(s) 642, 875, 1490, 1508
PciI ACATGT 1 cut(s) 1504
PcsI WCGNNNNNNNCGW 1 cut(s) 1237
PctI GAATGC 1 cut(s) 832
PdmI GAANNNNTTC 2 cut(s) 660, 1506
PfeI GAWTC 5 cut(s) 342, 413, 430, 1289, 1327
PkrI GCNGC 5 cut(s) 456, 570, 719, 722, 1360
PleI GAGTC 3 cut(s) 170, 260, 1236
PpsI GAGTC 3 cut(s) 170, 260, 1236
PscI ACATGT 1 cut(s) 1504
PshAI GACNNNNGTC 1 cut(s) 8
PsiI TTATAA 2 cut(s) 911, 1265
Psp6I CCWGG 2 cut(s) 420, 680
PspFI CCCAGC 1 cut(s) 1525
PspGI CCWGG 2 cut(s) 420, 680
PspPI GGNCC 1 cut(s) 604
PvuII CAGCTG 1 cut(s) 1433
RsaI GTAC 4 cut(s) 67, 521, 611, 860
RsaNI GTAC 4 cut(s) 66, 520, 610, 859
RseI CAYNNNNRTG 2 cut(s) 1203, 1335
SalI GTCGAC 1 cut(s) 1239
SaqAI TTAA 1 cut(s) 960
SatI GCNGC 5 cut(s) 455, 569, 718, 721, 1359
Sau3AI GATC 6 cut(s) 940, 1159, 1219, 1246, 1363, 1371
Sau96I GGNCC 1 cut(s) 604
ScaI AGTACT 1 cut(s) 521
SchI GAGTC 3 cut(s) 170, 260, 1236
ScrFI CCNGG 2 cut(s) 422, 682
SfaNI GCATC 5 cut(s) 625, 718, 957, 1050, 1503
SinI GGWCC 1 cut(s) 604
SmiMI CAYNNNNRTG 2 cut(s) 1203, 1335
SmlI CTYRAG 2 cut(s) 28, 1379
SmoI CTYRAG 2 cut(s) 28, 1379
Sse9I AATT 5 cut(s) 354, 534, 657, 1448, 1457
SspI AATATT 1 cut(s) 1420
SspMI CTAG 2 cut(s) 549, 1388
StyD4I CCNGG 2 cut(s) 420, 680
StyI CCWWGG 1 cut(s) 1345
TaaI ACNGT 3 cut(s) 388, 581, 676
TaiI ACGT 1 cut(s) 1357
TaqI TCGA 8 cut(s) 264, 614, 651, 976, 1240, 1245, 1271, 1374
TasI AATT 5 cut(s) 354, 534, 657, 1448, 1457
TatI WGTACW 3 cut(s) 65, 519, 609
TauI GCSGC 3 cut(s) 457, 720, 1361
TfiI GAWTC 5 cut(s) 342, 413, 430, 1289, 1327
Tru1I TTAA 1 cut(s) 960
Tru9I TTAA 1 cut(s) 960
TscAI CASTG 2 cut(s) 391, 679
TseFI GTSAC 1 cut(s) 97
TseI GCWGC 2 cut(s) 568, 720
Tsp45I GTSAC 1 cut(s) 97
TspRI CASTG 2 cut(s) 391, 679
VpaK11BI GGWCC 1 cut(s) 604
XapI RAATTY 3 cut(s) 354, 534, 657
XbaI TCTAGA 1 cut(s) 1387
XceI RCATGY 4 cut(s) 642, 875, 1490, 1508
XmiI GTMKAC 1 cut(s) 1240
XmnI GAANNNNTTC 2 cut(s) 660, 1506
XspI CTAG 2 cut(s) 549, 1388
ZrmI AGTACT 1 cut(s) 521
Zsp2I ATGCAT 1 cut(s) 640
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.