Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0016162

Physical Location & Seq

Genomic Coordinates Forward (+)

32881934 .. 32883733

Gene Structure 1800 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1193700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1800 bp

ATGACAGCGCTTCCAGTCGCAACCAAAGCTCAAACCTCGTACCAGAAACTACTTCAAACCTCTTCATCAACCGGTTTGCTGTCCCTCATCAAATCATGCACCACAAAATCCCATCTTCTCCAAATCCACGCCCATACCCTTCGAACATCACTCGTTCTGGACCCCACCATTTCCTTCCAGTTCCTGTCCGTCGTCGTCGCTCTTTCCCCGTTGACAAGCATCACATATTCCCGCCAATTCTTCGACCAAATCGAAAAGCCGACGGCTTTTCACTACAACAAAATGATAAGAGCTTACTCCAAGAGCGACTCGCCGGCGGAGGGGTTTTATCTGTACAGAGATTTGCGGCGGCGCGGCCTCTGCGCGGACGCTATGGCGTCGTCTTTTGTTATCAAGTCCTGCATTAGAGTTTCGTCGTTGGTGGGTGGGATTCAGGTGCATGCTAGGATTTTGAGAGATGGGCATCGATCAGACAGTCTTTTGCTGACCACTTTGATGGATTTGTATTCGAATTGCAGGAAGTATGATGAAGCGTGTCAGGTGTTTGATGAAATGCCCAAGAGAGATACTGTGGCTTGGAATGTATTGATTTCTTGTTGTTTGCATAACAACCGGACAAGGGATGCTTTGGGTTTGTTTGATATTATGCAGAGTGAGATACATAGGTGTGAGCCTGATGACGTTACGTGTTTGCTTGTGCTACAAGCTTGTGCCAGCTTGAACGCGTTGGAGTTTGGCGAACGGGTTCATAAGTACATTGAGGATCACGGTTACGGTGGTGCTTCAAATTTGTGCAACTCTCTTGTATCGATGTATTCACGTTGTGGGAGTTTGGATAAGGCCTATGGGGTTTTTAAGGGAATGCGGGATAAAAACGTGGTTTCGTGGAGTGCGATGATTTCAGGGTTGGCAGTGAATGGTTATGGTAGAGAAGCTATTGAAGCGTTTGAAGAGATGCAGAAAATGGGTGTTTTACCTGATGATCAGACTTTTACCGGAGTTCTTTGTGCTTGCAGCCATTGCGGTTTGGTTGATGAAGGAATGATGTTTTTCGATCTTATGAGCAAAGAGTTTGGGGTAGTGCCAAATGTCCATCACTATGGGTGCATGGTTGATCTTTTGGGTCGTGCTGGTCAACTTGACCCGGCCTATCAGCTCATAACGTCGATGGATATCAAACCAGATCCTACAATATGGAGGACCTTACTCGGGGCTTGCAGAATTCATGGAAATGATTCCCTTGCAGAACGAGTGGTAGGCCATTTGATTGAACTCAAGGCCCAAGAGGCTGGGGATTATGTTCTATTGATGAATATTTACTCTTCAGCTGGGAACTGGGAGAAGCTAACAGAAGTGAGGAAGTTTATGAAGGAAAAAGCAATTCAAACTACACCGGGATGTAGCACGATTGAACTGAAAGGAGTAGCACACGAGTTCGTTGTGGATGATGTTTCACATCTGCGGAAGGATGAAATCTACAAAATGCTGGATGAGATTAATAGCCAGCTGAAGATTGCTGGTTATGTTGCAGATGTAACATCTGAATTGCACAACTTGGGAGCAGAAGACAAGGGGCATGCCCTCTCTCACCACAGCGAGAAATTGGCTATTGCTTTCGGTGTTCTGTCAACTCCTCCCGGCACTCCAATCAGAGTGGCCAAGAATCTTCGCATTTGCGTTGATTGCCACAATTTCGCAATGGCTCTCTCAGGGGTTTACAACAGAGAGGTAATTATCAGAGACCGCACTCGGTTTCATCATTTCCGGGAGGGACACTGCTCCTGCAATGGCTATTGGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DYW_deaminase PPR PPR_1 PPR_2

Protein Analysis

600

Amino Acids

66.82

Weight (kDa)

6.68

Isoelectric Point (pI)

39.7

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	188 - 238	9.2e-09	PPR repeat family
PPR_2	PF13041	291 - 339	8.4e-12	PPR repeat family
PPR	PF01535	294 - 324	9.2e-08	PPR repeat
E_motif	PF20431	409 - 471	1.4e-15	E motif
DYW_deaminase	PF14432	507 - 599	2.5e-35	DYW family of nucleic acid deaminases

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0016162)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G47530
fragaria_vesca	FvH4_5g14900
malus_domestica	MD06G1193700.v1.1 MD14G1200800.v1.1
prunus_persica	Prupe.5G194000_v2.0.a1
rosa_chinensis	RchiOBHm_Chr7g0181481
rosa_laevigata	RLG00000005190
rosa_multiflora	Rmu_co8291233.1_g000001 Rmu_sc0002060.1_g000023
rosa_roxburghii	Rroxscaffold_3G00271960
rosa_rugosa	Rorug06G0441700
rosa_samantha	Rh7AG043500 Rh7BG042900 Rh7CG044700
rosa_wichuraiana	Rw7G003620

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccII	CGCG	3 cut(s)	354, 365, 725
AclWI	GGATC	2 cut(s)	771, 1178
AcoI	YGGCCR	1 cut(s)	1656
AcsI	RAATTY	2 cut(s)	787, 1221
AcuI	CTGAAG	2 cut(s)	1308, 1529
AcyI	GRCGYC	1 cut(s)	377
AdeI	CACNNNGTG	1 cut(s)	824
AfaI	GTAC	3 cut(s)	41, 335, 755
AfeI	AGCGCT	1 cut(s)	9
AfiI	CCNNNNNNNGG	4 cut(s)	320, 364, 619, 1209
AflIII	ACRYGT	2 cut(s)	686, 723
AgeI	ACCGGT	1 cut(s)	71
AgsI	TTSAA	8 cut(s)	56, 721, 786, 941, 950, 1271, 1385, 1412
AluBI	AGCT	9 cut(s)	29, 293, 707, 717, 935, 1156, 1328, 1345, 1507
AluI	AGCT	9 cut(s)	29, 293, 707, 717, 935, 1156, 1328, 1345, 1507
Alw26I	GTCTC	1 cut(s)	1734
AlwI	GGATC	2 cut(s)	771, 1178
AlwNI	CAGNNNCTG	1 cut(s)	184
Ama87I	CYCGRG	1 cut(s)	1208
Aor51HI	AGCGCT	1 cut(s)	9
AoxI	GGCC	6 cut(s)	355, 840, 1146, 1258, 1278, 1656
ApeKI	GCWGC	1 cut(s)	1014
ApoI	RAATTY	2 cut(s)	787, 1221
AseI	ATTAAT	1 cut(s)	1497
AsiGI	ACCGGT	1 cut(s)	71
Asp700I	GAANNNNTTC	2 cut(s)	744, 1234
AspLEI	GCGC	3 cut(s)	10, 354, 365
AspS9I	GGNCC	3 cut(s)	160, 1200, 1279
AsuC2I	CCSGG	4 cut(s)	1145, 1395, 1638, 1766
AsuHPI	GGTGA	1 cut(s)	1580
AsuII	TTCGAA	2 cut(s)	142, 509
AvaI	CYCGRG	1 cut(s)	1208
AvaII	GGWCC	2 cut(s)	160, 1200
BaeI	ACNNNNGTAYC	2 cut(s)	650, 683
BalI	TGGCCA	1 cut(s)	1658
BauI	CACGAG	1 cut(s)	1430
BbsI	GAAGAC	1 cut(s)	1572
BbvI	GCAGC	1 cut(s)	1026
BccI	CCATC	5 cut(s)	120, 452, 490, 1101, 1162
BceAI	ACGGC	1 cut(s)	279
BclI	TGATCA	1 cut(s)	982
BcnI	CCSGG	4 cut(s)	1145, 1395, 1638, 1766
BcoDI	GTCTC	1 cut(s)	1734
BfaI	CTAG	1 cut(s)	444
BfoI	RGCGCY	1 cut(s)	11
BglI	GCCNNNNNGGC	1 cut(s)	1286
BisI	GCNGC	4 cut(s)	347, 350, 355, 1015
BlsI	GCNGC	4 cut(s)	348, 351, 356, 1016
Bme1390I	CCNGG	4 cut(s)	1145, 1395, 1638, 1766
Bme18I	GGWCC	2 cut(s)	160, 1200
BmeT110I	CYCGRG	1 cut(s)	1208
BmgT120I	GGNCC	3 cut(s)	160, 1200, 1279
BmiI	GGNNCC	1 cut(s)	162
BmrFI	CCNGG	4 cut(s)	1145, 1395, 1638, 1766
BmrI	ACTGGG	1 cut(s)	1345
BmsI	GCATC	4 cut(s)	228, 472, 613, 945
BmuI	ACTGGG	1 cut(s)	1345
BpiI	GAAGAC	1 cut(s)	1572
Bpu14I	TTCGAA	2 cut(s)	142, 509
BpuEI	CTTGAG	1 cut(s)	1259
BpuMI	CCSGG	4 cut(s)	1145, 1395, 1638, 1766
Bsa29I	ATCGAT	2 cut(s)	466, 809
BsaAI	YACGTR	1 cut(s)	687
BsaBI	GATNNNNATC	2 cut(s)	462, 1172
BsaHI	GRCGYC	1 cut(s)	377
BsaI	GGTCTC	1 cut(s)	1734
BsaWI	WCCGGW	3 cut(s)	71, 612, 995
Bsc4I	CCNNNNNNNGG	4 cut(s)	320, 364, 619, 1209
Bse118I	RCCGGY	2 cut(s)	71, 313
Bse1I	ACTGG	3 cut(s)	14, 178, 1340
Bse3DI	GCAATG	3 cut(s)	1018, 1704, 1792
Bse8I	GATNNNNATC	2 cut(s)	462, 1172
BseCI	ATCGAT	2 cut(s)	466, 809
BseGI	GGATG	5 cut(s)	628, 1403, 1450, 1474, 1495
BseJI	GATNNNNATC	2 cut(s)	462, 1172
BseLI	CCNNNNNNNGG	4 cut(s)	320, 364, 619, 1209
BseMI	GCAATG	3 cut(s)	1018, 1704, 1792
BseMII	CTCAG	1 cut(s)	1722
BseNI	ACTGG	3 cut(s)	14, 178, 1340
BseRI	GAGGAG	1 cut(s)	1623
BseXI	GCAGC	1 cut(s)	1026
BseYI	CCCAGC	2 cut(s)	1289, 1328
Bsh1236I	CGCG	3 cut(s)	354, 365, 725
BshFI	GGCC	6 cut(s)	357, 842, 1148, 1260, 1280, 1658
BshTI	ACCGGT	1 cut(s)	71
BshVI	ATCGAT	2 cut(s)	466, 809
BsiHKCI	CYCGRG	1 cut(s)	1208
BsiSI	CCGG	8 cut(s)	72, 314, 613, 996, 1145, 1394, 1638, 1765
BslFI	GGGAC	2 cut(s)	67, 1785
BslI	CCNNNNNNNGG	4 cut(s)	320, 364, 619, 1209
BsmAI	GTCTC	1 cut(s)	1734
BsmFI	GGGAC	2 cut(s)	67, 1785
BsmI	GAATGC	1 cut(s)	867
BsnI	GGCC	6 cut(s)	357, 842, 1148, 1260, 1280, 1658
Bso31I	GGTCTC	1 cut(s)	1734
BsoBI	CYCGRG	1 cut(s)	1208
Bsp119I	TTCGAA	2 cut(s)	142, 509
Bsp1407I	TGTACA	1 cut(s)	333
Bsp143I	GATC	6 cut(s)	467, 763, 982, 1054, 1114, 1183
BspANI	GGCC	6 cut(s)	357, 842, 1148, 1260, 1280, 1658
BspCNI	CTCAG	1 cut(s)	1721
BspDI	ATCGAT	2 cut(s)	466, 809
BspFNI	CGCG	3 cut(s)	354, 365, 725
BspLI	GGNNCC	1 cut(s)	162
BspPI	GGATC	2 cut(s)	771, 1178
BspT104I	TTCGAA	2 cut(s)	142, 509
BspTNI	GGTCTC	1 cut(s)	1734
BsrDI	GCAATG	3 cut(s)	1018, 1704, 1792
BsrFI	RCCGGY	2 cut(s)	71, 313
BsrGI	TGTACA	1 cut(s)	333
BsrI	ACTGG	3 cut(s)	14, 178, 1340
BssAI	RCCGGY	2 cut(s)	71, 313
BssMI	GATC	6 cut(s)	467, 763, 982, 1054, 1114, 1183
BssNI	GRCGYC	1 cut(s)	377
BssSI	CACGAG	1 cut(s)	1430
Bst2BI	CACGAG	1 cut(s)	1430
Bst4CI	ACNGT	4 cut(s)	476, 571, 770, 776
Bst6I	CTCTTC	3 cut(s)	67, 945, 1327
BstACI	GRCGYC	1 cut(s)	377
BstAPI	GCANNNNNTGC	1 cut(s)	1020
BstAUI	TGTACA	1 cut(s)	333
BstBAI	YACGTR	1 cut(s)	687
BstBI	TTCGAA	2 cut(s)	142, 509
BstC8I	GCNNGC	7 cut(s)	315, 441, 715, 1012, 1216, 1505, 1578
BstDEI	CTNAG	1 cut(s)	1708
BstF5I	GGATG	5 cut(s)	628, 1403, 1450, 1474, 1495
BstFNI	CGCG	3 cut(s)	354, 365, 725
BstH2I	RGCGCY	1 cut(s)	11
BstHHI	GCGC	3 cut(s)	10, 354, 365
BstKTI	GATC	6 cut(s)	470, 766, 985, 1057, 1117, 1186
BstMAI	GTCTC	1 cut(s)	1734
BstMBI	GATC	6 cut(s)	467, 763, 982, 1054, 1114, 1183
BstMWI	GCNNNNNNNGC	6 cut(s)	26, 360, 941, 1020, 1286, 1683
BstNSI	RCATGY	2 cut(s)	443, 1580
BstSCI	CCNGG	4 cut(s)	1143, 1393, 1636, 1764
BstUI	CGCG	3 cut(s)	354, 365, 725
BstV1I	GCAGC	1 cut(s)	1026
BstV2I	GAAGAC	1 cut(s)	1572
BstX2I	RGATCY	1 cut(s)	1183
BstXI	CCANNNNNNTGG	3 cut(s)	496, 1100, 1289
BstYI	RGATCY	1 cut(s)	1183
Bsu15I	ATCGAT	2 cut(s)	466, 809
BsuRI	GGCC	6 cut(s)	357, 842, 1148, 1260, 1280, 1658
BsuTUI	ATCGAT	2 cut(s)	466, 809
BtgZI	GCGATG	1 cut(s)	908
BtsCI	GGATG	5 cut(s)	628, 1403, 1450, 1474, 1495
BtsI	GCAGTG	2 cut(s)	918, 1774
BtsIMutI	CAGTG	2 cut(s)	918, 1774
Cac8I	GCNNGC	7 cut(s)	315, 441, 715, 1012, 1216, 1505, 1578
CaiI	CAGNNNCTG	1 cut(s)	184
CfoI	GCGC	3 cut(s)	10, 354, 365
Cfr10I	RCCGGY	2 cut(s)	71, 313
Cfr13I	GGNCC	3 cut(s)	160, 1200, 1279
ClaI	ATCGAT	2 cut(s)	466, 809
CseI	GACGC	2 cut(s)	366, 377
Csp6I	GTAC	3 cut(s)	40, 334, 754
CspAI	ACCGGT	1 cut(s)	71
CspCI	CAANNNNNGTGG	2 cut(s)	1635, 1670
CviAII	CATG	5 cut(s)	96, 440, 1108, 1226, 1577
CviQI	GTAC	3 cut(s)	40, 334, 754
DdeI	CTNAG	1 cut(s)	1708
DpnI	GATC	6 cut(s)	469, 765, 984, 1056, 1116, 1185
DpnII	GATC	6 cut(s)	467, 763, 982, 1054, 1114, 1183
DraIII	CACNNNGTG	1 cut(s)	824
EaeI	YGGCCR	1 cut(s)	1656
Eam1104I	CTCTTC	3 cut(s)	67, 945, 1327
EarI	CTCTTC	3 cut(s)	67, 945, 1327
EciI	GGCGGA	1 cut(s)	332
Eco147I	AGGCCT	1 cut(s)	842
Eco31I	GGTCTC	1 cut(s)	1734
Eco32I	GATATC	1 cut(s)	1174
Eco47I	GGWCC	2 cut(s)	160, 1200
Eco47III	AGCGCT	1 cut(s)	9
Eco57I	CTGAAG	2 cut(s)	1308, 1529
Eco88I	CYCGRG	1 cut(s)	1208
EcoO109I	RGGNCCY	1 cut(s)	1200
EcoRI	GAATTC	1 cut(s)	1221
EcoRV	GATATC	1 cut(s)	1174
FaeI	CATG	5 cut(s)	99, 443, 1111, 1229, 1580
FalI	AAGNNNNNCTT	2 cut(s)	610, 642
FaqI	GGGAC	2 cut(s)	67, 1785
FatI	CATG	5 cut(s)	95, 439, 1107, 1225, 1576
FauI	CCCGC	2 cut(s)	239, 858
FbaI	TGATCA	1 cut(s)	982
Fnu4HI	GCNGC	4 cut(s)	347, 350, 355, 1015
FokI	GGATG	5 cut(s)	635, 1410, 1457, 1481, 1502
Fsp4HI	GCNGC	4 cut(s)	347, 350, 355, 1015
FspBI	CTAG	1 cut(s)	444
GlaI	GCGC	3 cut(s)	9, 353, 364
GluI	GCNGC	4 cut(s)	347, 350, 355, 1015
GsaI	CCCAGC	2 cut(s)	1293, 1332
HaeII	RGCGCY	1 cut(s)	11
HaeIII	GGCC	6 cut(s)	357, 842, 1148, 1260, 1280, 1658
HapII	CCGG	8 cut(s)	72, 314, 613, 996, 1145, 1394, 1638, 1765
HgaI	GACGC	2 cut(s)	366, 377
HhaI	GCGC	3 cut(s)	10, 354, 365
Hin1I	GRCGYC	1 cut(s)	377
Hin1II	CATG	5 cut(s)	99, 443, 1111, 1229, 1580
Hin6I	GCGC	3 cut(s)	8, 352, 363
HinP1I	GCGC	3 cut(s)	8, 352, 363
HincII	GTYRAC	3 cut(s)	213, 1136, 1629
HindII	GTYRAC	3 cut(s)	213, 1136, 1629
HindIII	AAGCTT	1 cut(s)	705
HinfI	GANTC	4 cut(s)	308, 430, 1235, 1663
HpaII	CCGG	8 cut(s)	72, 314, 613, 996, 1145, 1394, 1638, 1765
HphI	GGTGA	1 cut(s)	1580
Hpy166II	GTNNAC	4 cut(s)	213, 1136, 1629, 1717
Hpy188I	TCNGA	5 cut(s)	472, 987, 1543, 1652, 1739
Hpy188III	TCNNGA	1 cut(s)	158
Hpy8I	GTNNAC	4 cut(s)	213, 1136, 1629, 1717
Hpy99I	CGWCG	7 cut(s)	194, 197, 200, 265, 382, 418, 1168
HpyAV	CCTTC	5 cut(s)	149, 184, 1031, 1363, 1459
HpyCH4III	ACNGT	4 cut(s)	476, 571, 770, 776
HpyCH4IV	ACGT	5 cut(s)	681, 686, 820, 876, 1163
HpyF10VI	GCNNNNNNNGC	6 cut(s)	26, 360, 941, 1020, 1286, 1683
HpyF3I	CTNAG	1 cut(s)	1708
HpySE526I	ACGT	5 cut(s)	681, 686, 820, 876, 1163
Hsp92I	GRCGYC	1 cut(s)	377
Hsp92II	CATG	5 cut(s)	99, 443, 1111, 1229, 1580
HspAI	GCGC	3 cut(s)	8, 352, 363
KroI	GCCGGC	1 cut(s)	313
KroNI	GCCGGC	1 cut(s)	315
Ksp22I	TGATCA	1 cut(s)	982
Kzo9I	GATC	6 cut(s)	467, 763, 982, 1054, 1114, 1183
LmnI	GCTCC	2 cut(s)	1559, 1784
Lsp1109I	GCAGC	1 cut(s)	1026
LweI	GCATC	4 cut(s)	228, 472, 613, 945
MaeI	CTAG	1 cut(s)	444
MaeII	ACGT	5 cut(s)	681, 686, 820, 876, 1163
MaeIII	GTNAC	3 cut(s)	682, 770, 1534
MalI	GATC	6 cut(s)	469, 765, 984, 1056, 1116, 1185
MboI	GATC	6 cut(s)	467, 763, 982, 1054, 1114, 1183
MboII	GAAGA	8 cut(s)	54, 107, 232, 962, 1314, 1522, 1577, 1658
MflI	RGATCY	1 cut(s)	1183
MlsI	TGGCCA	1 cut(s)	1658
MluCI	AATT	9 cut(s)	236, 511, 787, 1221, 1380, 1544, 1601, 1690, 1731
MluI	ACGCGT	1 cut(s)	723
MluNI	TGGCCA	1 cut(s)	1658
MlyI	GAGTC	1 cut(s)	302
MmeI	TCCRAC	1 cut(s)	708
Mox20I	TGGCCA	1 cut(s)	1658
MreI	CGCCGGCG	1 cut(s)	313
MroNI	GCCGGC	1 cut(s)	313
MroXI	GAANNNNTTC	2 cut(s)	744, 1234
MscI	TGGCCA	1 cut(s)	1658
MseI	TTAA	2 cut(s)	855, 1497
MslI	CAYNNNNRTG	5 cut(s)	494, 666, 1098, 1230, 1396
Msp20I	TGGCCA	1 cut(s)	1658
MspA1I	CMGCKG	2 cut(s)	1328, 1507
MspI	CCGG	8 cut(s)	72, 314, 613, 996, 1145, 1394, 1638, 1765
MspR9I	CCNGG	4 cut(s)	1145, 1395, 1638, 1766
Mva1269I	GAATGC	1 cut(s)	867
MvnI	CGCG	3 cut(s)	354, 365, 725
MwoI	GCNNNNNNNGC	6 cut(s)	26, 360, 941, 1020, 1286, 1683
NaeI	GCCGGC	1 cut(s)	315
NciI	CCSGG	4 cut(s)	1145, 1395, 1638, 1766
NdeII	GATC	6 cut(s)	467, 763, 982, 1054, 1114, 1183
NgoMIV	GCCGGC	1 cut(s)	313
NlaIII	CATG	5 cut(s)	99, 443, 1111, 1229, 1580
NlaIV	GGNNCC	1 cut(s)	162
NspI	RCATGY	2 cut(s)	443, 1580
NspV	TTCGAA	2 cut(s)	142, 509
PaeI	GCATGC	2 cut(s)	443, 1580
PceI	AGGCCT	1 cut(s)	842
PcsI	WCGNNNNNNNCGW	2 cut(s)	249, 890
PctI	GAATGC	1 cut(s)	867
PdiI	GCCGGC	1 cut(s)	315
PdmI	GAANNNNTTC	2 cut(s)	744, 1234
PfeI	GAWTC	3 cut(s)	430, 1235, 1663
PfoI	TCCNGGA	1 cut(s)	1764
PinAI	ACCGGT	1 cut(s)	71
PkrI	GCNGC	4 cut(s)	348, 351, 356, 1016
PleI	GAGTC	1 cut(s)	302
PpsI	GAGTC	1 cut(s)	302
Ppu21I	YACGTR	1 cut(s)	687
PpuMI	RGGWCCY	1 cut(s)	1200
PshBI	ATTAAT	1 cut(s)	1497
Psp5II	RGGWCCY	1 cut(s)	1200
PspFI	CCCAGC	2 cut(s)	1289, 1328
PspN4I	GGNNCC	1 cut(s)	162
PspPI	GGNCC	3 cut(s)	160, 1200, 1279
PspPPI	RGGWCCY	1 cut(s)	1200
PstNI	CAGNNNCTG	1 cut(s)	184
PsuI	RGATCY	1 cut(s)	1183
PvuII	CAGCTG	2 cut(s)	1328, 1507
RsaI	GTAC	3 cut(s)	41, 335, 755
RsaNI	GTAC	3 cut(s)	40, 334, 754
RseI	CAYNNNNRTG	5 cut(s)	494, 666, 1098, 1230, 1396
SaqAI	TTAA	2 cut(s)	855, 1497
SatI	GCNGC	4 cut(s)	347, 350, 355, 1015
Sau3AI	GATC	6 cut(s)	467, 763, 982, 1054, 1114, 1183
Sau96I	GGNCC	3 cut(s)	160, 1200, 1279
SchI	GAGTC	1 cut(s)	302
ScrFI	CCNGG	4 cut(s)	1145, 1395, 1638, 1766
SfaNI	GCATC	4 cut(s)	228, 472, 613, 945
SfuI	TTCGAA	2 cut(s)	142, 509
SgrAI	CRCCGGYG	1 cut(s)	313
SinI	GGWCC	2 cut(s)	160, 1200
SmiMI	CAYNNNNRTG	5 cut(s)	494, 666, 1098, 1230, 1396
SmlI	CTYRAG	1 cut(s)	1274
SmoI	CTYRAG	1 cut(s)	1274
SphI	GCATGC	2 cut(s)	443, 1580
Sse9I	AATT	9 cut(s)	236, 511, 787, 1221, 1380, 1544, 1601, 1690, 1731
SseBI	AGGCCT	1 cut(s)	842
SspI	AATATT	1 cut(s)	1315
SspMI	CTAG	1 cut(s)	444
StuI	AGGCCT	1 cut(s)	842
StyD4I	CCNGG	4 cut(s)	1143, 1393, 1636, 1764
TaaI	ACNGT	4 cut(s)	476, 571, 770, 776
TaiI	ACGT	5 cut(s)	684, 689, 823, 879, 1166
TaqI	TCGA	8 cut(s)	142, 243, 252, 466, 509, 809, 1053, 1166
TasI	AATT	9 cut(s)	236, 511, 787, 1221, 1380, 1544, 1601, 1690, 1731
TatI	WGTACW	2 cut(s)	333, 753
TauI	GCSGC	3 cut(s)	349, 352, 357
TfiI	GAWTC	3 cut(s)	430, 1235, 1663
Tru1I	TTAA	2 cut(s)	855, 1497
Tru9I	TTAA	2 cut(s)	855, 1497
TscAI	CASTG	2 cut(s)	918, 1781
TseI	GCWGC	1 cut(s)	1014
TspGWI	ACGGA	1 cut(s)	178
TspRI	CASTG	2 cut(s)	918, 1781
VpaK11BI	GGWCC	2 cut(s)	160, 1200
VspI	ATTAAT	1 cut(s)	1497
XapI	RAATTY	2 cut(s)	787, 1221
XceI	RCATGY	2 cut(s)	443, 1580
XmnI	GAANNNNTTC	2 cut(s)	744, 1234
XspI	CTAG	1 cut(s)	444

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.