FvH4_5g14940

Exostosin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0007835
Physical Location & Seq
Reverse (-)
8451943 .. 8454997
3055 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g14940.t1

Sequence Viewer

Length: 1482 bp
ATGTCATCAATGTCAGAAAAGAGTAGGATTCCCTCAAAGTTCATTTCCTCCCTAATAACAGTTTCTGGGTTCCTTTTAATTCTTTCTTCTGTGTTCCTTCTTCAATCTGGCAATACATCTTTCATTCCCAGATCAGTGTTTGAGCTTATGCTTGTTAATGGCACATCTGTGTACTTTGAGCCCCATGCGGAAAAGGAACCTATTAAATGTTCTTTCCTCTCTTCTGAGGTTCCCCAAGTAGATGCTATACAACCCTTGAAAAGAATGAGTGAATCAGCTTGTCAAGTTTCCAATCCAAGCAAGAAAGTGGGATCTGTTGGTAGAAAGAACATGGTTTGTGACTCGAAGCAGGCTCTTTTGAGGGTATTTATGTATGACTTGCCTCCTGAATTTCACTTTGGGTTATTGGGTTGGAAAGAAATGGAGAATCAAACATGGCCACAGCACATCCCACCTTACCCAGGCGGGCTGAATTTACAGCACAGTATAGAATATTGGCTCACCCTTGATATTTTATCATCAGATAACGTAAATGTGGTTAGACCTTGTACATTAGTCAGGGTGCACAATCCAAGAGAAGCTGATTTTATTTTTGTGCCATTCTTCGCATCTCTGAGTTACAACCGGCATTCTAAACTTCATGAGAAGGAGAAAGTCAGTGTCAACAAGAGGTTGCAGGACAAGTTGGTGCAGTTTTTGATGGGCCGGAATGAATGGAAAAGAAAGGGTGGGAAAGATCATTTAATAGTTGCTCATCATCCAAATAGCATGTTGGATGCAAGAAAGAAATTGGGTTCTGCCATGTTCGTGCTTGCAGATTTTGGAAGATATCCTGCTGAAATAGCAAATCTTGGGAAGGATATAATCGCTCCTTACAGACATTTGGTGAAGACTGTTCCCAGTAGCAAATCAGCCACATTCAAGGAGCGTCCTATACTAGTGTATTTCCGAGGAGCAATATACAGAAAAGATGGAGGAGTAATTCGACAAGAATTGTATTATCTTCTAAAAGATGAGAAAGATGTTGACTTCAATTTTGGTAGCATTGGAGGAAACGGTGTTCGCAAGGCAACCCAAGGAATGGCCTCATCGAAATTTTGCTTAAATATAGCTGGCGATACCCCATCCTCAAATCGCTTATTCGATGCCATTGCTAGTCATTGTGTTCCAGTGATAATCAGTGATGAGATTGAGGTACCATTTGAAGATGTCTTAGACTACTCACAGTTTTGCATATTTGTTCGTGGGGATGATGCTATAAAGAAGGGTTACCTGCTGAAACTTCTTCGAGGAATAAATGAAGAGAAGTGGACCAAAATGTGGGAAAGGTTGAAGGAAGTTGTACATCATTTCGAATATCAGTATCCCTCCCTGCCTGGCGATGCTGTTGATATGATTTGGCAGGCAGTTTCTCGCAAGATATCATCTGTACAATTTAAATATAACAGGAAGAGGAGATATGACAGGTCTCAACTTGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exostosin

Protein Analysis

494

Amino Acids

56.29

Weight (kDa)

9.31

Isoelectric Point (pI)

42.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exostosin_GT47 PF03016 118 - 425 2.2e-60 Exostosin GT47 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1281
Acc65I GGTACC 1 cut(s) 1195
AccB1I GGYRCC 1 cut(s) 1195
AccB7I CCANNNNNTGG 2 cut(s) 1081, 1320
AciI CCGC 2 cut(s) 188, 465
AclWI GGATC 1 cut(s) 319
AcoI YGGCCR 1 cut(s) 437
AcsI RAATTY 3 cut(s) 389, 472, 1094
AfaI GTAC 5 cut(s) 173, 550, 1197, 1344, 1431
AfiI CCNNNNNNNGG 3 cut(s) 461, 1081, 1320
AgsI TTSAA 6 cut(s) 104, 259, 922, 1033, 1205, 1333
AhlI ACTAGT 1 cut(s) 937
AjnI CCWGG 2 cut(s) 460, 1375
AjuI GAANNNNNNNTTGG 2 cut(s) 381, 413
AleI CACNNNNGTG 1 cut(s) 167
AloI GAACNNNNNNTCC 2 cut(s) 1044, 1076
AluBI AGCT 4 cut(s) 145, 278, 581, 1112
AluI AGCT 4 cut(s) 145, 278, 581, 1112
Alw21I GWGCWC 1 cut(s) 567
Alw26I GTCTC 1 cut(s) 1473
Alw44I GTGCAC 1 cut(s) 563
AlwI GGATC 1 cut(s) 319
AlwNI CAGNNNCTG 1 cut(s) 65
AoxI GGCC 3 cut(s) 437, 703, 1083
ApaLI GTGCAC 1 cut(s) 563
ApoI RAATTY 3 cut(s) 389, 472, 1094
ArsI GACNNNNNNTTYG 2 cut(s) 1019, 1051
Asp718I GGTACC 1 cut(s) 1195
AspS9I GGNCC 2 cut(s) 703, 1311
AsuHPI GGTGA 2 cut(s) 493, 898
AsuII TTCGAA 1 cut(s) 1353
AvaII GGWCC 1 cut(s) 1311
BaeGI GKGCMC 1 cut(s) 567
BalI TGGCCA 1 cut(s) 439
BanI GGYRCC 1 cut(s) 1195
BanII GRGCYC 1 cut(s) 183
BbsI GAAGAC 1 cut(s) 896
Bbv12I GWGCWC 1 cut(s) 567
BccI CCATC 3 cut(s) 694, 965, 1132
BcgI CGANNNNNNTGC 2 cut(s) 1133, 1167
BciT130I CCWGG 2 cut(s) 462, 1377
BciVI GTATCC 1 cut(s) 1374
BcoDI GTCTC 1 cut(s) 1473
BcuI ACTAGT 1 cut(s) 937
BfaI CTAG 2 cut(s) 938, 1155
BfuAI ACCTGC 1 cut(s) 1281
BfuI GTATCC 1 cut(s) 1374
Bme1390I CCNGG 2 cut(s) 462, 1377
Bme18I GGWCC 1 cut(s) 1311
BmgT120I GGNCC 2 cut(s) 703, 1311
BmiI GGNNCC 4 cut(s) 71, 198, 231, 1197
BmrFI CCNGG 2 cut(s) 462, 1377
BmrI ACTGGG 1 cut(s) 894
BmsI GCATC 6 cut(s) 232, 617, 766, 1135, 1243, 1372
BmuI ACTGGG 1 cut(s) 894
BpiI GAAGAC 1 cut(s) 896
Bpu14I TTCGAA 1 cut(s) 1353
BsaI GGTCTC 1 cut(s) 1473
BsaJI CCNNGG 3 cut(s) 460, 949, 1075
Bsc4I CCNNNNNNNGG 3 cut(s) 461, 1081, 1320
Bse118I RCCGGY 1 cut(s) 624
Bse1I ACTGG 2 cut(s) 900, 1169
Bse3DI GCAATG 1 cut(s) 1149
BseBI CCWGG 2 cut(s) 462, 1377
BseDI CCNNGG 3 cut(s) 460, 949, 1075
BseGI GGATG 5 cut(s) 447, 757, 781, 1124, 1255
BseLI CCNNNNNNNGG 3 cut(s) 461, 1081, 1320
BseMI GCAATG 1 cut(s) 1149
BseMII CTCAG 2 cut(s) 216, 605
BseNI ACTGG 2 cut(s) 900, 1169
BseRI GAGGAG 3 cut(s) 966, 990, 1468
BseSI GKGCMC 1 cut(s) 567
BsgI GTGCAG 1 cut(s) 710
BshFI GGCC 3 cut(s) 439, 705, 1085
BshNI GGYRCC 1 cut(s) 1195
BsiHKAI GWGCWC 1 cut(s) 567
BsiSI CCGG 2 cut(s) 625, 706
BslI CCNNNNNNNGG 3 cut(s) 461, 1081, 1320
BsmAI GTCTC 1 cut(s) 1473
BsmI GAATGC 1 cut(s) 628
BsnI GGCC 3 cut(s) 439, 705, 1085
Bso31I GGTCTC 1 cut(s) 1473
Bsp119I TTCGAA 1 cut(s) 1353
Bsp1286I GDGCHC 2 cut(s) 183, 567
Bsp1407I TGTACA 3 cut(s) 548, 1342, 1429
Bsp143I GATC 3 cut(s) 131, 311, 736
BspACI CCGC 2 cut(s) 188, 465
BspANI GGCC 3 cut(s) 439, 705, 1085
BspCNI CTCAG 2 cut(s) 217, 606
BspHI TCATGA 1 cut(s) 640
BspLI GGNNCC 4 cut(s) 71, 198, 231, 1197
BspMI ACCTGC 1 cut(s) 1281
BspPI GGATC 1 cut(s) 319
BspT104I TTCGAA 1 cut(s) 1353
BspT107I GGYRCC 1 cut(s) 1195
BspTNI GGTCTC 1 cut(s) 1473
BsrDI GCAATG 1 cut(s) 1149
BsrFI RCCGGY 1 cut(s) 624
BsrGI TGTACA 3 cut(s) 548, 1342, 1429
BsrI ACTGG 2 cut(s) 900, 1169
BssAI RCCGGY 1 cut(s) 624
BssECI CCNNGG 3 cut(s) 460, 949, 1075
BssMI GATC 3 cut(s) 131, 311, 736
BssT1I CCWWGG 1 cut(s) 1075
Bst2UI CCWGG 2 cut(s) 462, 1377
Bst4CI ACNGT 5 cut(s) 61, 485, 895, 1058, 1227
Bst6I CTCTTC 3 cut(s) 226, 1296, 1445
BstAUI TGTACA 3 cut(s) 548, 1342, 1429
BstBI TTCGAA 1 cut(s) 1353
BstC8I GCNNGC 5 cut(s) 351, 467, 813, 1114, 1404
BstDEI CTNAG 3 cut(s) 225, 614, 1213
BstEII GGTNACC 1 cut(s) 1268
BstENI CCTNNNNNAGG 1 cut(s) 459
BstF5I GGATG 5 cut(s) 447, 757, 781, 1124, 1255
BstKTI GATC 3 cut(s) 134, 314, 739
BstMAI GTCTC 1 cut(s) 1473
BstMBI GATC 3 cut(s) 131, 311, 736
BstMWI GCNNNNNNNGC 1 cut(s) 842
BstNI CCWGG 2 cut(s) 462, 1377
BstNSI RCATGY 1 cut(s) 772
BstPI GGTNACC 1 cut(s) 1268
BstSCI CCNGG 2 cut(s) 460, 1375
BstSLI GKGCMC 1 cut(s) 567
BstV2I GAAGAC 1 cut(s) 896
BstX2I RGATCY 1 cut(s) 311
BstYI RGATCY 1 cut(s) 311
BsuI GTATCC 1 cut(s) 1374
BsuRI GGCC 3 cut(s) 439, 705, 1085
BtgZI GCGATG 1 cut(s) 1395
BtsCI GGATG 5 cut(s) 447, 757, 781, 1124, 1255
BtsIMutI CAGTG 4 cut(s) 141, 664, 1176, 1186
BveI ACCTGC 1 cut(s) 1281
Cac8I GCNNGC 5 cut(s) 351, 467, 813, 1114, 1404
CaiI CAGNNNCTG 1 cut(s) 65
CciI TCATGA 1 cut(s) 640
Cfr10I RCCGGY 1 cut(s) 624
Cfr13I GGNCC 2 cut(s) 703, 1311
CseI GACGC 1 cut(s) 917
Csp6I GTAC 5 cut(s) 172, 549, 1196, 1343, 1430
CviAII CATG 6 cut(s) 185, 331, 435, 641, 769, 802
CviQI GTAC 5 cut(s) 172, 549, 1196, 1343, 1430
DdeI CTNAG 3 cut(s) 225, 614, 1213
DpnI GATC 3 cut(s) 133, 313, 738
DpnII GATC 3 cut(s) 131, 311, 736
DraI TTTAAA 1 cut(s) 1438
EaeI YGGCCR 1 cut(s) 437
Eam1104I CTCTTC 3 cut(s) 226, 1296, 1445
EarI CTCTTC 3 cut(s) 226, 1296, 1445
Eco130I CCWWGG 1 cut(s) 1075
Eco24I GRGCYC 1 cut(s) 183
Eco31I GGTCTC 1 cut(s) 1473
Eco32I GATATC 2 cut(s) 830, 1422
Eco47I GGWCC 1 cut(s) 1311
Eco91I GGTNACC 1 cut(s) 1268
EcoNI CCTNNNNNAGG 1 cut(s) 459
EcoO65I GGTNACC 1 cut(s) 1268
EcoRII CCWGG 2 cut(s) 460, 1375
EcoRV GATATC 2 cut(s) 830, 1422
EcoT14I CCWWGG 1 cut(s) 1075
EcoT38I GRGCYC 1 cut(s) 183
ErhI CCWWGG 1 cut(s) 1075
FaeI CATG 6 cut(s) 188, 334, 438, 644, 772, 805
FatI CATG 6 cut(s) 184, 330, 434, 640, 768, 801
FauI CCCGC 1 cut(s) 458
FokI GGATG 5 cut(s) 434, 744, 788, 1111, 1262
FriOI GRGCYC 1 cut(s) 183
FspBI CTAG 2 cut(s) 938, 1155
HaeIII GGCC 3 cut(s) 439, 705, 1085
HapII CCGG 2 cut(s) 625, 706
HgaI GACGC 1 cut(s) 917
Hin1II CATG 6 cut(s) 188, 334, 438, 644, 772, 805
HincII GTYRAC 2 cut(s) 664, 1027
HindII GTYRAC 2 cut(s) 664, 1027
HinfI GANTC 4 cut(s) 28, 272, 341, 427
HpaII CCGG 2 cut(s) 625, 706
HphI GGTGA 2 cut(s) 493, 898
Hpy166II GTNNAC 5 cut(s) 172, 565, 664, 1027, 1311
Hpy188I TCNGA 5 cut(s) 16, 226, 523, 615, 950
Hpy188III TCNNGA 2 cut(s) 386, 641
Hpy8I GTNNAC 5 cut(s) 172, 565, 664, 1027, 1311
HpyAV CCTTC 5 cut(s) 107, 640, 850, 1258, 1327
HpyCH4III ACNGT 5 cut(s) 61, 485, 895, 1058, 1227
HpyCH4IV ACGT 1 cut(s) 528
HpyCH4V TGCA 6 cut(s) 565, 676, 691, 779, 815, 1233
HpyF10VI GCNNNNNNNGC 1 cut(s) 842
HpyF3I CTNAG 3 cut(s) 225, 614, 1213
HpySE526I ACGT 1 cut(s) 528
Hsp92II CATG 6 cut(s) 188, 334, 438, 644, 772, 805
KpnI GGTACC 1 cut(s) 1199
Kzo9I GATC 3 cut(s) 131, 311, 736
LmnI GCTCC 3 cut(s) 874, 925, 953
LweI GCATC 6 cut(s) 232, 617, 766, 1135, 1243, 1372
MaeI CTAG 2 cut(s) 938, 1155
MaeII ACGT 1 cut(s) 528
MaeIII GTNAC 3 cut(s) 338, 617, 1268
MalI GATC 3 cut(s) 133, 313, 738
MboI GATC 3 cut(s) 131, 311, 736
MflI RGATCY 1 cut(s) 311
MhlI GDGCHC 2 cut(s) 183, 567
MlsI TGGCCA 1 cut(s) 439
MluCI AATT 9 cut(s) 78, 389, 472, 788, 981, 992, 1033, 1094, 1433
MluNI TGGCCA 1 cut(s) 439
MlyI GAGTC 1 cut(s) 335
MmeI TCCRAC 2 cut(s) 392, 753
Mox20I TGGCCA 1 cut(s) 439
MscI TGGCCA 1 cut(s) 439
MseI TTAA 7 cut(s) 77, 156, 204, 743, 1103, 1437, 1480
MslI CAYNNNNRTG 2 cut(s) 167, 806
Msp20I TGGCCA 1 cut(s) 439
MspI CCGG 2 cut(s) 625, 706
MspR9I CCNGG 2 cut(s) 462, 1377
Mva1269I GAATGC 1 cut(s) 628
MvaI CCWGG 2 cut(s) 462, 1377
MwoI GCNNNNNNNGC 1 cut(s) 842
NdeII GATC 3 cut(s) 131, 311, 736
NlaIII CATG 6 cut(s) 188, 334, 438, 644, 772, 805
NlaIV GGNNCC 4 cut(s) 71, 198, 231, 1197
NmuCI GTSAC 1 cut(s) 338
NspI RCATGY 1 cut(s) 772
NspV TTCGAA 1 cut(s) 1353
OliI CACNNNNGTG 1 cut(s) 167
PagI TCATGA 1 cut(s) 640
PctI GAATGC 1 cut(s) 628
PfeI GAWTC 3 cut(s) 28, 272, 427
PflMI CCANNNNNTGG 2 cut(s) 1081, 1320
PleI GAGTC 1 cut(s) 335
PpsI GAGTC 1 cut(s) 335
Psp6I CCWGG 2 cut(s) 460, 1375
PspEI GGTNACC 1 cut(s) 1268
PspGI CCWGG 2 cut(s) 460, 1375
PspN4I GGNNCC 4 cut(s) 71, 198, 231, 1197
PspPI GGNCC 2 cut(s) 703, 1311
PstNI CAGNNNCTG 1 cut(s) 65
PsuI RGATCY 1 cut(s) 311
RsaI GTAC 5 cut(s) 173, 550, 1197, 1344, 1431
RsaNI GTAC 5 cut(s) 172, 549, 1196, 1343, 1430
RseI CAYNNNNRTG 2 cut(s) 167, 806
SaqAI TTAA 7 cut(s) 77, 156, 204, 743, 1103, 1437, 1480
Sau3AI GATC 3 cut(s) 131, 311, 736
Sau96I GGNCC 2 cut(s) 703, 1311
SchI GAGTC 1 cut(s) 335
ScrFI CCNGG 2 cut(s) 462, 1377
SduI GDGCHC 2 cut(s) 183, 567
SfaNI GCATC 6 cut(s) 232, 617, 766, 1135, 1243, 1372
SfuI TTCGAA 1 cut(s) 1353
SinI GGWCC 1 cut(s) 1311
SmiI ATTTAAAT 1 cut(s) 1438
SmiMI CAYNNNNRTG 2 cut(s) 167, 806
SpeI ACTAGT 1 cut(s) 937
Sse9I AATT 9 cut(s) 78, 389, 472, 788, 981, 992, 1033, 1094, 1433
SsiI CCGC 2 cut(s) 188, 465
SspI AATATT 1 cut(s) 494
SspMI CTAG 2 cut(s) 938, 1155
StyD4I CCNGG 2 cut(s) 460, 1375
StyI CCWWGG 1 cut(s) 1075
SwaI ATTTAAAT 1 cut(s) 1438
TaaI ACNGT 5 cut(s) 61, 485, 895, 1058, 1227
TaiI ACGT 1 cut(s) 531
TaqI TCGA 6 cut(s) 344, 985, 1091, 1143, 1288, 1353
TasI AATT 9 cut(s) 78, 389, 472, 788, 981, 992, 1033, 1094, 1433
TatI WGTACW 4 cut(s) 171, 548, 1342, 1429
TfiI GAWTC 3 cut(s) 28, 272, 427
Tru1I TTAA 7 cut(s) 77, 156, 204, 743, 1103, 1437, 1480
Tru9I TTAA 7 cut(s) 77, 156, 204, 743, 1103, 1437, 1480
TscAI CASTG 4 cut(s) 141, 664, 1176, 1186
TseFI GTSAC 1 cut(s) 338
Tsp45I GTSAC 1 cut(s) 338
TspDTI ATGAA 5 cut(s) 31, 112, 629, 726, 1314
TspRI CASTG 4 cut(s) 141, 664, 1176, 1186
Van91I CCANNNNNTGG 2 cut(s) 1081, 1320
VneI GTGCAC 1 cut(s) 563
VpaK11BI GGWCC 1 cut(s) 1311
XagI CCTNNNNNAGG 1 cut(s) 459
XapI RAATTY 3 cut(s) 389, 472, 1094
XceI RCATGY 1 cut(s) 772
XspI CTAG 2 cut(s) 938, 1155
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.