MD06G1193300.v1.1

Exostosin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0007835
Physical Location & Seq
Forward (+)
32863055 .. 32866156
3102 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1193300.v1.1.491

Sequence Viewer

Length: 1500 bp
ATGTCGGAAAAGAGTAGGCTTCCATCGAAGTTTCTTTTCTACTTGATAACGATTTCATTGTTCCTTTTAATCTCTTCTTCTGTCTTCCTACTTCAGTATAGCAATACTTCCTTTACACCGAGTTCAGTGTTTAAGCTTATTCTTCTCAATGGCACGTCGGTGTACTTTGAGCCCCTTTTGAAAAGTAGGCAGAAAAACCTTCTTTTCCTCCCTTCTGAGGTTCCCCTAGTTGATGCTGGAATATCCACAACAATAAGTGAATTGGGATGTGAAAATTCCAATTCAAGCAAGAAGTTGGAGTCTTTAGGAAAAAAGAAAACGATTGCTTGTGACTCGACTCAGGCTCTCTTGAGGGTGTTTATGTATGAGTTGCCTTCAGAGTTTCACTTTGGGTTATTGGATTGGAAGGGTAAGGAGAATCAAACTTGGCCAAATGTCAAAAACCCGAGTCATATTCCAGCTTACCCAGGCGGCCTGAATTTACAGCACAGTATCGAATACTGGCTCACCCTTGATCTTCTATCATCAAGTCTTCCGACTTTGGTCAGACCGTGCACGGTAGTCAGGGTGCACAATTCAAGTCAAGCAGATGTTATTTTTGTGCCGTTCTTCGCATCTCTGAGTTACAACAGGCATTCTAAGCTTCGTGGAAAGGAGAAAGTAAGTGTGGACAAGAAGTTGCAGGAAGAGTTGGTGCAGTATTTGATGGGTCGGGATGAATGGAAAAGAAAGGGTGGGAAGGATCATTTAATAGTTGCTCACCATCCCAATAGCATGTTAGATGCAAGAAAGAAATTAGGCTCTGCAATGTTTGTGCTTGCGGATTTTGGAAGATACCCTGCTGAAATAGCAAATATTGAGAAGGACATAATTGCTCCTTACAGACATTTGGTGATGACTGTTCACAGCAGCAAATCAGCCTCATTCAATGAGCGTCCTATACTTTTATACTTCCGAGGAGCAATATACAGGAAAGATGGAGGAAAAGTTCGACAGGAATTGTATTATCTTCTTAAAGATGAGAAAGATGTATACTTCACTTTTGGTAGCGTTCGAGGGAATGGTATTAACAAGGCAAGCCAAGGAATGGCCTCATCAAAGTTTTGCCTGAATCTTGCTGGTGATACCCCTTCCTCAAATCGCCTATTTGATGCCATCGCCAGCCATTGTGTTCCTGTGATAATTAGCGATGAGATTGAGCTACCATTTGAAGATTTCTTAGACTACTCACAGTTTTGCATATTTGTTCACTCAGCTGACGCTATTAAGAAGGGTTACCTACTGAATCTTCTTCGAGGAATCAAGCAGGAAACATGGACCAAGATGTGGGAAAAGTTGAAGGAAGTTGTACATCATTTTGAATATCAGTATCCATCCCAGCCTGGTGATGCCGTTGATATGATATGGCAGGCAGTTGCTCATAAGAAAACCTCTGTACAATTTAAATTTCACAGGAAGAAGAGATATGACAGGTCAACATCTGATATAAGAGCGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exostosin

Protein Analysis

500

Amino Acids

56.93

Weight (kDa)

9.32

Isoelectric Point (pI)

38.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exostosin_GT47 PF03016 116 - 428 3.1e-60 Exostosin GT47 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 1087, 1326
AccI GTMKAC 1 cut(s) 1032
AciI CCGC 2 cut(s) 471, 821
AclWI GGATC 1 cut(s) 750
AcoI YGGCCR 1 cut(s) 428
AcsI RAATTY 3 cut(s) 274, 478, 1445
AcuI CTGAAG 2 cut(s) 77, 360
AfaI GTAC 3 cut(s) 164, 1350, 1437
AfiI CCNNNNNNNGG 3 cut(s) 217, 1087, 1326
AgsI TTSAA 7 cut(s) 181, 285, 579, 928, 1211, 1339, 1361
AjiI CACGTC 1 cut(s) 156
AjnI CCWGG 2 cut(s) 466, 1381
AleI CACNNNNGTG 1 cut(s) 158
AloI GAACNNNNNNTCC 2 cut(s) 972, 1004
AluBI AGCT 5 cut(s) 136, 461, 643, 1201, 1256
AluI AGCT 5 cut(s) 136, 461, 643, 1201, 1256
Alw21I GWGCWC 2 cut(s) 557, 573
Alw44I GTGCAC 2 cut(s) 553, 569
AlwI GGATC 1 cut(s) 750
Ama87I CYCGRG 1 cut(s) 445
AoxI GGCC 3 cut(s) 428, 472, 1089
ApaLI GTGCAC 2 cut(s) 553, 569
ApeKI GCWGC 1 cut(s) 909
ApoI RAATTY 3 cut(s) 274, 478, 1445
AspS9I GGNCC 1 cut(s) 1317
AsuHPI GGTGA 5 cut(s) 499, 752, 904, 1133, 1397
AvaI CYCGRG 1 cut(s) 445
AvaII GGWCC 1 cut(s) 1317
BaeGI GKGCMC 2 cut(s) 557, 573
BalI TGGCCA 1 cut(s) 430
BanII GRGCYC 1 cut(s) 174
BbsI GAAGAC 2 cut(s) 76, 524
Bbv12I GWGCWC 2 cut(s) 557, 573
BbvI GCAGC 1 cut(s) 921
BccI CCATC 6 cut(s) 31, 700, 771, 971, 1163, 1381
BceAI ACGGC 2 cut(s) 589, 1376
BciT130I CCWGG 2 cut(s) 468, 1383
BciVI GTATCC 1 cut(s) 1380
BfaI CTAG 1 cut(s) 227
BfuI GTATCC 1 cut(s) 1380
BisI GCNGC 2 cut(s) 472, 910
BlsI GCNGC 2 cut(s) 473, 911
Bme1390I CCNGG 2 cut(s) 468, 1383
Bme18I GGWCC 1 cut(s) 1317
BmeT110I CYCGRG 1 cut(s) 445
BmgBI CACGTC 1 cut(s) 156
BmgT120I GGNCC 1 cut(s) 1317
BmiI GGNNCC 1 cut(s) 222
BmrFI CCNGG 2 cut(s) 468, 1383
BmsI GCATC 5 cut(s) 223, 623, 772, 1141, 1378
BoxI GACNNNNGTC 1 cut(s) 542
BpiI GAAGAC 2 cut(s) 76, 524
BpuEI CTTGAG 1 cut(s) 370
BsaJI CCNNGG 3 cut(s) 466, 955, 1081
BsaXI ACNNNNNCTCC 2 cut(s) 972, 1002
Bsc4I CCNNNNNNNGG 3 cut(s) 217, 1087, 1326
Bse1I ACTGG 1 cut(s) 506
Bse3DI GCAATG 1 cut(s) 813
BseBI CCWGG 2 cut(s) 468, 1383
BseDI CCNNGG 3 cut(s) 466, 955, 1081
BseGI GGATG 4 cut(s) 272, 721, 763, 1373
BseLI CCNNNNNNNGG 3 cut(s) 217, 1087, 1326
BseMI GCAATG 1 cut(s) 813
BseMII CTCAG 4 cut(s) 207, 353, 611, 1266
BseNI ACTGG 1 cut(s) 506
BseRI GAGGAG 1 cut(s) 972
BseSI GKGCMC 2 cut(s) 557, 573
BseXI GCAGC 1 cut(s) 921
BseYI CCCAGC 1 cut(s) 1377
BsgI GTGCAG 1 cut(s) 716
BshFI GGCC 3 cut(s) 430, 474, 1091
BsiHKAI GWGCWC 2 cut(s) 557, 573
BsiHKCI CYCGRG 1 cut(s) 445
BslI CCNNNNNNNGG 3 cut(s) 217, 1087, 1326
BsmI GAATGC 1 cut(s) 634
BsnI GGCC 3 cut(s) 430, 474, 1091
BsoBI CYCGRG 1 cut(s) 445
Bsp1286I GDGCHC 3 cut(s) 174, 557, 573
Bsp1407I TGTACA 2 cut(s) 1348, 1435
Bsp143I GATC 2 cut(s) 514, 742
BspACI CCGC 2 cut(s) 471, 821
BspANI GGCC 3 cut(s) 430, 474, 1091
BspCNI CTCAG 4 cut(s) 208, 352, 612, 1265
BspLI GGNNCC 1 cut(s) 222
BspPI GGATC 1 cut(s) 750
BsrDI GCAATG 1 cut(s) 813
BsrGI TGTACA 2 cut(s) 1348, 1435
BsrI ACTGG 1 cut(s) 506
BssECI CCNNGG 3 cut(s) 466, 955, 1081
BssMI GATC 2 cut(s) 514, 742
BssNAI GTATAC 1 cut(s) 1033
BssT1I CCWWGG 1 cut(s) 1081
Bst1107I GTATAC 1 cut(s) 1033
Bst2UI CCWGG 2 cut(s) 468, 1383
Bst4CI ACNGT 5 cut(s) 491, 552, 559, 901, 1233
Bst6I CTCTTC 3 cut(s) 79, 681, 1454
BstAUI TGTACA 2 cut(s) 1348, 1435
BstC8I GCNNGC 4 cut(s) 819, 1078, 1162, 1410
BstDEI CTNAG 6 cut(s) 216, 339, 620, 639, 1219, 1252
BstEII GGTNACC 1 cut(s) 1274
BstF5I GGATG 4 cut(s) 272, 721, 763, 1373
BstKTI GATC 2 cut(s) 517, 745
BstMBI GATC 2 cut(s) 514, 742
BstMWI GCNNNNNNNGC 2 cut(s) 640, 848
BstNI CCWGG 2 cut(s) 468, 1383
BstNSI RCATGY 1 cut(s) 778
BstPAI GACNNNNGTC 1 cut(s) 542
BstPI GGTNACC 1 cut(s) 1274
BstSCI CCNGG 2 cut(s) 466, 1381
BstSLI GKGCMC 2 cut(s) 557, 573
BstV1I GCAGC 1 cut(s) 921
BstV2I GAAGAC 2 cut(s) 76, 524
BstZ17I GTATAC 1 cut(s) 1033
BsuI GTATCC 1 cut(s) 1380
BsuRI GGCC 3 cut(s) 430, 474, 1091
BtgZI GCGATG 2 cut(s) 1141, 1203
BtrI CACGTC 1 cut(s) 156
BtsCI GGATG 4 cut(s) 272, 721, 763, 1373
BtsIMutI CAGTG 1 cut(s) 132
Cac8I GCNNGC 4 cut(s) 819, 1078, 1162, 1410
Cfr13I GGNCC 1 cut(s) 1317
CseI GACGC 2 cut(s) 923, 1268
Csp6I GTAC 3 cut(s) 163, 1349, 1436
CviAII CATG 2 cut(s) 775, 1314
CviQI GTAC 3 cut(s) 163, 1349, 1436
DdeI CTNAG 6 cut(s) 216, 339, 620, 639, 1219, 1252
DpnI GATC 2 cut(s) 516, 744
DpnII GATC 2 cut(s) 514, 742
DraI TTTAAA 1 cut(s) 1444
EaeI YGGCCR 1 cut(s) 428
Eam1104I CTCTTC 3 cut(s) 79, 681, 1454
EarI CTCTTC 3 cut(s) 79, 681, 1454
Eco130I CCWWGG 1 cut(s) 1081
Eco24I GRGCYC 1 cut(s) 174
Eco47I GGWCC 1 cut(s) 1317
Eco57I CTGAAG 2 cut(s) 77, 360
Eco88I CYCGRG 1 cut(s) 445
Eco91I GGTNACC 1 cut(s) 1274
EcoO65I GGTNACC 1 cut(s) 1274
EcoRII CCWGG 2 cut(s) 466, 1381
EcoT14I CCWWGG 1 cut(s) 1081
EcoT38I GRGCYC 1 cut(s) 174
ErhI CCWWGG 1 cut(s) 1081
FaeI CATG 2 cut(s) 778, 1317
FatI CATG 2 cut(s) 774, 1313
FblI GTMKAC 1 cut(s) 1032
Fnu4HI GCNGC 2 cut(s) 472, 910
FokI GGATG 4 cut(s) 279, 728, 750, 1360
FriOI GRGCYC 1 cut(s) 174
Fsp4HI GCNGC 2 cut(s) 472, 910
FspBI CTAG 1 cut(s) 227
GluI GCNGC 2 cut(s) 472, 910
GsaI CCCAGC 1 cut(s) 1381
HaeIII GGCC 3 cut(s) 430, 474, 1091
HgaI GACGC 2 cut(s) 923, 1268
Hin1II CATG 2 cut(s) 778, 1317
HincII GTYRAC 1 cut(s) 1476
HindII GTYRAC 1 cut(s) 1476
HindIII AAGCTT 2 cut(s) 134, 641
HinfI GANTC 8 cut(s) 299, 332, 337, 418, 448, 1111, 1285, 1299
HphI GGTGA 5 cut(s) 499, 752, 904, 1133, 1397
Hpy166II GTNNAC 8 cut(s) 163, 555, 571, 670, 904, 1033, 1249, 1476
Hpy188I TCNGA 8 cut(s) 7, 217, 379, 537, 548, 621, 956, 1483
Hpy188III TCNNGA 2 cut(s) 349, 713
Hpy8I GTNNAC 8 cut(s) 163, 555, 571, 670, 904, 1033, 1249, 1476
Hpy99I CGWCG 1 cut(s) 160
HpyAV CCTTC 9 cut(s) 209, 222, 384, 400, 733, 856, 1140, 1264, 1333
HpyCH4III ACNGT 5 cut(s) 491, 552, 559, 901, 1233
HpyCH4IV ACGT 1 cut(s) 155
HpyCH4V TGCA 7 cut(s) 555, 571, 682, 697, 785, 806, 1239
HpyF10VI GCNNNNNNNGC 2 cut(s) 640, 848
HpyF3I CTNAG 6 cut(s) 216, 339, 620, 639, 1219, 1252
HpySE526I ACGT 1 cut(s) 155
Hsp92II CATG 2 cut(s) 778, 1317
Kzo9I GATC 2 cut(s) 514, 742
LmnI GCTCC 2 cut(s) 880, 959
Lsp1109I GCAGC 1 cut(s) 921
LweI GCATC 5 cut(s) 223, 623, 772, 1141, 1378
MaeI CTAG 1 cut(s) 227
MaeII ACGT 1 cut(s) 155
MaeIII GTNAC 3 cut(s) 329, 623, 1274
MalI GATC 2 cut(s) 516, 744
MboI GATC 2 cut(s) 514, 742
MhlI GDGCHC 3 cut(s) 174, 557, 573
MlsI TGGCCA 1 cut(s) 430
MluNI TGGCCA 1 cut(s) 430
MlyI GAGTC 4 cut(s) 308, 326, 331, 457
MmeI TCCRAC 2 cut(s) 276, 560
Mox20I TGGCCA 1 cut(s) 430
MscI TGGCCA 1 cut(s) 430
MseI TTAA 7 cut(s) 68, 132, 749, 1014, 1068, 1266, 1443
MslI CAYNNNNRTG 1 cut(s) 158
Msp20I TGGCCA 1 cut(s) 430
MspA1I CMGCKG 1 cut(s) 1256
MspR9I CCNGG 2 cut(s) 468, 1383
Mva1269I GAATGC 1 cut(s) 634
MvaI CCWGG 2 cut(s) 468, 1383
MwoI GCNNNNNNNGC 2 cut(s) 640, 848
NdeII GATC 2 cut(s) 514, 742
NlaIII CATG 2 cut(s) 778, 1317
NlaIV GGNNCC 1 cut(s) 222
NmuCI GTSAC 1 cut(s) 329
NspI RCATGY 1 cut(s) 778
OliI CACNNNNGTG 1 cut(s) 158
PctI GAATGC 1 cut(s) 634
PfeI GAWTC 4 cut(s) 418, 1111, 1285, 1299
PflMI CCANNNNNTGG 2 cut(s) 1087, 1326
PkrI GCNGC 2 cut(s) 473, 911
PleI GAGTC 4 cut(s) 307, 326, 331, 456
PpsI GAGTC 4 cut(s) 307, 326, 331, 456
PshAI GACNNNNGTC 1 cut(s) 542
Psp6I CCWGG 2 cut(s) 466, 1381
PspEI GGTNACC 1 cut(s) 1274
PspFI CCCAGC 1 cut(s) 1377
PspGI CCWGG 2 cut(s) 466, 1381
PspN4I GGNNCC 1 cut(s) 222
PspPI GGNCC 1 cut(s) 1317
PvuII CAGCTG 1 cut(s) 1256
RsaI GTAC 3 cut(s) 164, 1350, 1437
RsaNI GTAC 3 cut(s) 163, 1349, 1436
RseI CAYNNNNRTG 1 cut(s) 158
SaqAI TTAA 7 cut(s) 68, 132, 749, 1014, 1068, 1266, 1443
SatI GCNGC 2 cut(s) 472, 910
Sau3AI GATC 2 cut(s) 514, 742
Sau96I GGNCC 1 cut(s) 1317
SchI GAGTC 4 cut(s) 308, 326, 331, 457
ScrFI CCNGG 2 cut(s) 468, 1383
SduI GDGCHC 3 cut(s) 174, 557, 573
SfaNI GCATC 5 cut(s) 223, 623, 772, 1141, 1378
SinI GGWCC 1 cut(s) 1317
SmiI ATTTAAAT 1 cut(s) 1444
SmiMI CAYNNNNRTG 1 cut(s) 158
SmlI CTYRAG 1 cut(s) 349
SmoI CTYRAG 1 cut(s) 349
SsiI CCGC 2 cut(s) 471, 821
SspI AATATT 1 cut(s) 856
SspMI CTAG 1 cut(s) 227
StyD4I CCNGG 2 cut(s) 466, 1381
StyI CCWWGG 1 cut(s) 1081
SwaI ATTTAAAT 1 cut(s) 1444
TaaI ACNGT 5 cut(s) 491, 552, 559, 901, 1233
TaiI ACGT 1 cut(s) 158
TaqI TCGA 6 cut(s) 26, 335, 495, 991, 1054, 1294
TatI WGTACW 3 cut(s) 162, 1348, 1435
TauI GCSGC 1 cut(s) 474
TfiI GAWTC 4 cut(s) 418, 1111, 1285, 1299
Tru1I TTAA 7 cut(s) 68, 132, 749, 1014, 1068, 1266, 1443
Tru9I TTAA 7 cut(s) 68, 132, 749, 1014, 1068, 1266, 1443
TscAI CASTG 1 cut(s) 132
TseFI GTSAC 1 cut(s) 329
TseI GCWGC 1 cut(s) 909
Tsp45I GTSAC 1 cut(s) 329
TspDTI ATGAA 2 cut(s) 45, 732
TspRI CASTG 1 cut(s) 132
Van91I CCANNNNNTGG 2 cut(s) 1087, 1326
VneI GTGCAC 2 cut(s) 553, 569
VpaK11BI GGWCC 1 cut(s) 1317
XapI RAATTY 3 cut(s) 274, 478, 1445
XceI RCATGY 1 cut(s) 778
XmiI GTMKAC 1 cut(s) 1032
XspI CTAG 1 cut(s) 227
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.