FvH4_5g17490

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0001818
Physical Location & Seq
Forward (+)
10008666 .. 10014592
5927 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g17490.t14

Sequence Viewer

Length: 2868 bp
ATGGCGATTGTGACCGGAGATCGCTACCTGGAGAAGCTGGTCCAGTTTGTGGAGAAGCACGCCGGCTCTCTGATCGACGGCTCGTTAGTGCTGAAGCTCAACCCAGCCGGCTTCCACTACGTCCACTCGCGCCTCGAGGCCTTGCACGAGCTCGAGAGCCTCCTCGCCGGCGCTCCGGTAGATTATCTCCGAGCCTATGTTTCCGACCTCGGCGACCACCGCGCGCTCGAGCAGCTTCGGAGGATTCTTAGGCTCCTTACCTCGCTTAAGGTCGTCTCGGTGCTGCCGGCTCCCGGTAGGGACCCCACGCCGCTGTCGTTTTGGCCGTTCGGGAGGCTTAGGGTTTTGGAGCTTCGCGGATGCGATTTGTCCACGTCGGCGGCGAAAGGTCTGCTCGAGTTGAGGCACACCTTGGAGAAGATCGTCTGTCACAATTCCACTGATGCTCTGCGGCATGTGTTTGCGAGTAGAATTGCAGAGATTAAGCACTCGCCGGTGTGGAACCGGTTGTCATTTGTTTCGTGTGCTTGTAATGGCCTGGTTCTGATGGATGAGTCTTTGCAACTACTTCCCGTAGTGGAAACTCTTGACCTGAGCCGAAACAAGTTTGCAATGGTTGACAATCTTCGCAAGTGTGGCAAATTAAAACACCTAGACCTTGGTTTCAATCATCTGAGAACGATTGCGTCAATCGGTGAGGTCACCTCTCGACTAATTAAACTTGTTTTGAGGAACAATGCTCTATCTTCATTGCGTGGGATTGAGAATTTGAAGTCACTTGAAGCGCTTGATGTTTCCTACAATATTATTTCCAATTTTTCAGAGTTAGAGTTCCTTGGTGGTCTTCCATCTCTACAAAGCTTGTGGTTGGAAGGGAATCCATTATGTTGTGCCAGTTGGTATCGGTCACAAGTATTCAGCTATTTCTCTAATCCCGAAAAATTGAAGTTAGATGATAAGGAAATCAGCACCAGAGAGTTCTGGAAGAGGCAACTAATTATTGCAAGTAGACAGAAGCGACCTGCAAGCTTTGGGTTTTATTCCCCTGCAAAGTGTGATGATAAAGGAGATGCAAGTATCAACAGAAGAAGGAAAAAGGTGTCACGTCTTGCTTCCATTGTAAGTGAAGAAGGGAGCACATCTCTATGTTCTGATCAGGAGTCTGCGTCTTGTGACAATGAGATTCAAAGCAGAGAAGAACTTGTTATATCTGATGATGAAGCTGAAATTGTTGATTTGATGACTAGAGTAGAGCTAATGAAAAAGGAGCGCTCTGTTCTTTGGTTGCGGGAGTTCAAAGAATGGTTGGATCATGCTCCTGAGGATTCTGTGGACAACAATAGGCATGGTGGGATGACACTGCATAGTGGCAAGGAAAATTATATAAAAGAAAAGGCAAGCTGGATGCAGCTTGGTGTCAACTCCAGATTTATTTCAGATTACGCACATTCCTCAGGAGATGAGAGAAGTACAAATGTTTTAGATTCAGATAGTTCTTTCTTGGATATGTCCACTGGTCTGCATGCCCATCACTTTGATCAAATTGGTTCCTTGGGGAATGCTGGTTTCGCCAAGGATACCCTAAAAGACAATTCACATGAAGGAACGAGTAATGTCCCTTTACAAGCAAAAAGTTTCCATGGTCACATATTCACCAGTCAAAAAGGTCGTAGGATGGTGGAAAATTTGAGTATGTCGTTGAGTTCTATAGATGATATTTCTGAATCTCGCTCATCGTCTGTGTTCCCGGGATCGCCACCTCATTATCAAAAGGACATTCTTCATCGTCGTCATAACTTAGAGGAAGAAATTTTACAGTTGTCTGCAGAGTCATTTTCAGTGGCATCTTCTGATAGTAATACGAGCTGCAGTGAAGATGACCACTGTGAGTCTAGGCACTCCATTCCTGAAGGTCATCAGCTACTAAATAAGAGTGTGGAAGAAAATCTGTCTTCAGATCCTTTTAGGTTGTATGACATGAGATATGAGGTTCCACCTGTAAGAGGAAGTGACAGGAGTTCAGTTGGAATAGGTGCAGAGAAAATTTCAAACTCAGACCAGTCGCTGCAATCACATGCCAGTGTTCCAGGTCACACTCATGATGGTGAGATTGCTCATTTTGTTGATGAAGAAGGTGATTTAGAGAGGACAAAACACAGACAGAAAATAAAAAGAAGAGTTGTTACTCTACTAGAAGACGAAATTATGGTTAGGCAAGTAGAAACATTACCAACAATAAATGGTAGTATGGAAAATCATATAACGAAACTGGAAGATGAGCAAGAGAGTCGATCTTTTTATGGGGTTAACTTTGATGAAGTTATTGGTAAGAACCAGATGGTGGCAAATACAAGTAATATCCCACTTCCAAATGATAATACTGGATCTTCTGGGGCAGAGTGTTGCTCTTCAAGGAGTGATGAATTTATTGAGGACTATTTCAAAAAGAGTGTTGCAGACATGGGAAATAATGAAATTTGCAAGCAGTACATACGCTGCTATTGTATACTTGAGCAGGATTCCTTATATAGAGAAAGAGAAGTAGCTGTCTTGCTGAGCAGTGAAAACAAAGTGTATGTGCTACTAATTGGTACTGGAGGTGATGGATCAGGCACTATCTTGAATTTGCAGGGTTCTCATAGTGTTGAAGACATAAGAGAAGTGGTAGTTAGCTTAGGACTTCAGGTTGTGAGGGTGTTTTTCGGGGAGAGCGCAACCTACCTGTTTAAAACCAGAAGCATTGAAAGTTCCAGACAATTACTTTCGACTTTAACAGTTATCGATTCTTATTCTCCAATCGATAAATTTTGTTTGAGAAGGTTCAGGTCAGATTGTTTGAGAAACAAATATGTGGAGGTTCTAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_4

Protein Analysis

956

Amino Acids

107.32

Weight (kDa)

5.82

Isoelectric Point (pI)

55.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_4 PF12799 55 - 94 6.1e-06 Leucine Rich repeats (2 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 134
Acc36I ACCTGC 1 cut(s) 1030
AccB7I CCANNNNNTGG 2 cut(s) 49, 2341
AccI GTMKAC 2 cut(s) 1009, 2506
AccII CGCG 4 cut(s) 130, 222, 224, 357
AciI CCGC 6 cut(s) 220, 311, 357, 380, 451, 1288
AclWI GGATC 5 cut(s) 1317, 1759, 1952, 2392, 2614
AcoI YGGCCR 1 cut(s) 323
AcsI RAATTY 8 cut(s) 766, 1684, 1809, 2043, 2423, 2475, 2623, 2804
AcuI CTGAAG 4 cut(s) 113, 1929, 1938, 2666
AfaI GTAC 3 cut(s) 1471, 2489, 2593
AfeI AGCGCT 2 cut(s) 786, 1271
AfiI CCNNNNNNNGG 4 cut(s) 49, 293, 2003, 2341
AflII CTTAAG 1 cut(s) 266
AgeI ACCGGT 1 cut(s) 504
AjiI CACGTC 2 cut(s) 375, 1106
AjnI CCWGG 3 cut(s) 27, 537, 2086
Alw21I GWGCWC 2 cut(s) 153, 1139
Alw26I GTCTC 1 cut(s) 280
AlwI GGATC 5 cut(s) 1317, 1759, 1952, 2392, 2614
AlwNI CAGNNNCTG 1 cut(s) 2065
Ama87I CYCGRG 5 cut(s) 134, 152, 227, 395, 1747
Aor51HI AGCGCT 2 cut(s) 786, 1271
AoxI GGCC 3 cut(s) 138, 323, 535
ApeKI GCWGC 6 cut(s) 232, 283, 1408, 1866, 2065, 2496
ApoI RAATTY 8 cut(s) 766, 1684, 1809, 2043, 2423, 2475, 2623, 2804
AsiGI ACCGGT 1 cut(s) 504
AspLEI GCGC 7 cut(s) 132, 173, 224, 226, 787, 1272, 2714
AspS9I GGNCC 2 cut(s) 40, 301
AsuC2I CCSGG 3 cut(s) 294, 1748, 1749
AsuHPI GGTGA 6 cut(s) 694, 707, 1645, 2117, 2147, 2612
AvaI CYCGRG 5 cut(s) 134, 152, 227, 395, 1747
AvaII GGWCC 2 cut(s) 40, 301
AxyI CCTNAGG 2 cut(s) 1320, 1453
BanII GRGCYC 1 cut(s) 153
BarI GAAGNNNNNNTAC 4 cut(s) 1797, 1829, 2167, 2199
BauI CACGAG 1 cut(s) 146
BbsI GAAGAC 4 cut(s) 836, 1944, 2202, 2655
Bbv12I GWGCWC 2 cut(s) 153, 1139
BbvI GCAGC 6 cut(s) 244, 270, 1420, 1853, 2052, 2483
BccI CCATC 7 cut(s) 541, 856, 1536, 1669, 2096, 2332, 2597
BceAI ACGGC 2 cut(s) 94, 310
BcgI CGANNNNNNTGC 4 cut(s) 373, 407, 2270, 2304
BciT130I CCWGG 3 cut(s) 29, 539, 2088
BciVI GTATCC 1 cut(s) 1570
BclI TGATCA 2 cut(s) 1153, 1537
BcnI CCSGG 3 cut(s) 294, 1748, 1749
BcoDI GTCTC 1 cut(s) 280
BfaI CTAG 4 cut(s) 653, 1245, 1893, 2192
BfmI CTRYAG 3 cut(s) 1707, 1824, 1867
BfoI RGCGCY 3 cut(s) 174, 788, 1273
BfrI CTTAAG 1 cut(s) 266
BfuAI ACCTGC 1 cut(s) 1030
BfuI GTATCC 1 cut(s) 1570
BisI GCNGC 9 cut(s) 233, 284, 311, 381, 452, 1409, 1867, 2066, 2497
BlpI GCTNAGC 1 cut(s) 2555
BlsI GCNGC 9 cut(s) 234, 285, 312, 382, 453, 1410, 1868, 2067, 2498
Bme1390I CCNGG 6 cut(s) 29, 294, 539, 1748, 1749, 2088
Bme18I GGWCC 2 cut(s) 40, 301
BmeT110I CYCGRG 5 cut(s) 134, 152, 227, 395, 1747
BmgBI CACGTC 2 cut(s) 375, 1106
BmgT120I GGNCC 2 cut(s) 40, 301
BmiI GGNNCC 7 cut(s) 254, 291, 302, 303, 503, 1549, 1992
BmrFI CCNGG 6 cut(s) 29, 294, 539, 1748, 1749, 2088
BmsI GCATC 5 cut(s) 350, 433, 1060, 1395, 1853
BpiI GAAGAC 4 cut(s) 836, 1944, 2202, 2655
BplI GAGNNNNNCTC 6 cut(s) 689, 721, 1126, 1158, 2390, 2422
BpmI CTGGAG 3 cut(s) 50, 1408, 2616
Bpu10I CCTNAGC 3 cut(s) 338, 593, 2674
Bpu1102I GCTNAGC 1 cut(s) 2555
BpuEI CTTGAG 1 cut(s) 2531
BpuMI CCSGG 3 cut(s) 294, 1748, 1749
Bsa29I ATCGAT 2 cut(s) 2781, 2799
BsaJI CCNNGG 8 cut(s) 208, 411, 658, 835, 1551, 1572, 1639, 1747
BsaWI WCCGGW 3 cut(s) 14, 175, 504
BsaXI ACNNNNNCTCC 2 cut(s) 1151, 1181
Bsc4I CCNNNNNNNGG 4 cut(s) 49, 293, 2003, 2341
Bse118I RCCGGY 6 cut(s) 62, 107, 167, 286, 493, 504
Bse1I ACTGG 9 cut(s) 43, 894, 1519, 1656, 2059, 2079, 2274, 2386, 2599
Bse21I CCTNAGG 2 cut(s) 1320, 1453
Bse3DI GCAATG 2 cut(s) 618, 749
BseBI CCWGG 3 cut(s) 29, 539, 2088
BseCI ATCGAT 2 cut(s) 2781, 2799
BseDI CCNNGG 8 cut(s) 208, 411, 658, 835, 1551, 1572, 1639, 1747
BseGI GGATG 5 cut(s) 365, 556, 1359, 1410, 1680
BseLI CCNNNNNNNGG 4 cut(s) 49, 293, 2003, 2341
BseMI GCAATG 2 cut(s) 618, 749
BseMII CTCAG 6 cut(s) 584, 665, 1311, 1467, 2067, 2546
BseNI ACTGG 9 cut(s) 43, 894, 1519, 1656, 2059, 2079, 2274, 2386, 2599
BsePI GCGCGC 1 cut(s) 222
BseRI GAGGAG 1 cut(s) 152
BseXI GCAGC 6 cut(s) 244, 270, 1420, 1853, 2052, 2483
BseYI CCCAGC 1 cut(s) 103
BsgI GTGCAG 1 cut(s) 2055
Bsh1236I CGCG 4 cut(s) 130, 222, 224, 357
BshFI GGCC 3 cut(s) 140, 325, 537
BshTI ACCGGT 1 cut(s) 504
BshVI ATCGAT 2 cut(s) 2781, 2799
BsiHKAI GWGCWC 2 cut(s) 153, 1139
BsiHKCI CYCGRG 5 cut(s) 134, 152, 227, 395, 1747
BslFI GGGAC 2 cut(s) 314, 1601
BslI CCNNNNNNNGG 4 cut(s) 49, 293, 2003, 2341
BsmAI GTCTC 1 cut(s) 280
BsmBI CGTCTC 1 cut(s) 280
BsmFI GGGAC 2 cut(s) 314, 1601
BsmI GAATGC 1 cut(s) 1564
BsnI GGCC 3 cut(s) 140, 325, 537
BsoBI CYCGRG 5 cut(s) 134, 152, 227, 395, 1747
Bsp1286I GDGCHC 2 cut(s) 153, 1139
Bsp1720I GCTNAGC 1 cut(s) 2555
Bsp19I CCATGG 1 cut(s) 1639
BspACI CCGC 6 cut(s) 220, 311, 357, 380, 451, 1288
BspANI GGCC 3 cut(s) 140, 325, 537
BspCNI CTCAG 6 cut(s) 585, 666, 1312, 1466, 2066, 2547
BspDI ATCGAT 2 cut(s) 2781, 2799
BspFNI CGCG 4 cut(s) 130, 222, 224, 357
BspHI TCATGA 1 cut(s) 2098
BspLI GGNNCC 7 cut(s) 254, 291, 302, 303, 503, 1549, 1992
BspMAI CTGCAG 2 cut(s) 1828, 1871
BspMI ACCTGC 1 cut(s) 1030
BspPI GGATC 5 cut(s) 1317, 1759, 1952, 2392, 2614
BspQI GCTCTTC 1 cut(s) 2413
BspTI CTTAAG 1 cut(s) 266
BsrDI GCAATG 2 cut(s) 618, 749
BsrFI RCCGGY 6 cut(s) 62, 107, 167, 286, 493, 504
BsrI ACTGG 9 cut(s) 43, 894, 1519, 1656, 2059, 2079, 2274, 2386, 2599
BssAI RCCGGY 6 cut(s) 62, 107, 167, 286, 493, 504
BssECI CCNNGG 8 cut(s) 208, 411, 658, 835, 1551, 1572, 1639, 1747
BssHII GCGCGC 1 cut(s) 222
BssNAI GTATAC 1 cut(s) 2507
BssSI CACGAG 1 cut(s) 146
BssT1I CCWWGG 6 cut(s) 411, 658, 835, 1551, 1572, 1639
Bst1107I GTATAC 1 cut(s) 2507
Bst2BI CACGAG 1 cut(s) 146
Bst2UI CCWGG 3 cut(s) 29, 539, 2088
Bst4CI ACNGT 3 cut(s) 1818, 1886, 2776
Bst6I CTCTTC 3 cut(s) 980, 2170, 2413
BstAFI CTTAAG 1 cut(s) 266
BstDSI CCRYGG 1 cut(s) 1639
BstEII GGTNACC 1 cut(s) 700
BstENI CCTNNNNNAGG 1 cut(s) 2001
BstF5I GGATG 5 cut(s) 365, 556, 1359, 1410, 1680
BstFNI CGCG 4 cut(s) 130, 222, 224, 357
BstH2I RGCGCY 3 cut(s) 174, 788, 1273
BstHHI GCGC 7 cut(s) 132, 173, 224, 226, 787, 1272, 2714
BstMAI GTCTC 1 cut(s) 280
BstMWI GCNNNNNNNGC 4 cut(s) 219, 232, 636, 1568
BstNI CCWGG 3 cut(s) 29, 539, 2088
BstNSI RCATGY 3 cut(s) 458, 1526, 2078
BstPI GGTNACC 1 cut(s) 700
BstSCI CCNGG 6 cut(s) 27, 292, 537, 1746, 1747, 2086
BstSFI CTRYAG 3 cut(s) 1707, 1824, 1867
BstUI CGCG 4 cut(s) 130, 222, 224, 357
BstV1I GCAGC 6 cut(s) 244, 270, 1420, 1853, 2052, 2483
BstV2I GAAGAC 4 cut(s) 836, 1944, 2202, 2655
BstX2I RGATCY 2 cut(s) 1957, 2384
BstYI RGATCY 2 cut(s) 1957, 2384
BstZ17I GTATAC 1 cut(s) 2507
Bsu15I ATCGAT 2 cut(s) 2781, 2799
Bsu36I CCTNAGG 2 cut(s) 1320, 1453
BsuI GTATCC 1 cut(s) 1570
BsuRI GGCC 3 cut(s) 140, 325, 537
BsuTUI ATCGAT 2 cut(s) 2781, 2799
BtgI CCRYGG 1 cut(s) 1639
BtrI CACGTC 2 cut(s) 375, 1106
BtsCI GGATG 5 cut(s) 365, 556, 1359, 1410, 1680
BtsI GCAGTG 3 cut(s) 1358, 1876, 2566
BtsIMutI CAGTG 8 cut(s) 438, 1358, 1512, 1845, 1876, 1882, 2086, 2566
BveI ACCTGC 1 cut(s) 1030
CaiI CAGNNNCTG 1 cut(s) 2065
CciI TCATGA 1 cut(s) 2098
CfoI GCGC 7 cut(s) 132, 173, 224, 226, 787, 1272, 2714
Cfr10I RCCGGY 6 cut(s) 62, 107, 167, 286, 493, 504
Cfr13I GGNCC 2 cut(s) 40, 301
Cfr9I CCCGGG 1 cut(s) 1747
ClaI ATCGAT 2 cut(s) 2781, 2799
CseI GACGC 2 cut(s) 675, 1155
Csp6I GTAC 3 cut(s) 1470, 2488, 2592
CspAI ACCGGT 1 cut(s) 504
CspCI CAANNNNNGTGG 2 cut(s) 845, 880
CviQI GTAC 3 cut(s) 1470, 2488, 2592
DraI TTTAAA 1 cut(s) 2728
EaeI YGGCCR 1 cut(s) 323
Eam1104I CTCTTC 3 cut(s) 980, 2170, 2413
EarI CTCTTC 3 cut(s) 980, 2170, 2413
Ecl136II GAGCTC 1 cut(s) 151
Eco130I CCWWGG 6 cut(s) 411, 658, 835, 1551, 1572, 1639
Eco147I AGGCCT 1 cut(s) 140
Eco24I GRGCYC 1 cut(s) 153
Eco47I GGWCC 2 cut(s) 40, 301
Eco47III AGCGCT 2 cut(s) 786, 1271
Eco53kI GAGCTC 1 cut(s) 151
Eco57I CTGAAG 4 cut(s) 113, 1929, 1938, 2666
Eco81I CCTNAGG 2 cut(s) 1320, 1453
Eco88I CYCGRG 5 cut(s) 134, 152, 227, 395, 1747
Eco91I GGTNACC 1 cut(s) 700
EcoICRI GAGCTC 1 cut(s) 151
EcoNI CCTNNNNNAGG 1 cut(s) 2001
EcoO109I RGGNCCY 1 cut(s) 301
EcoO65I GGTNACC 1 cut(s) 700
EcoRII CCWGG 3 cut(s) 27, 537, 2086
EcoT14I CCWWGG 6 cut(s) 411, 658, 835, 1551, 1572, 1639
EcoT38I GRGCYC 1 cut(s) 153
ErhI CCWWGG 6 cut(s) 411, 658, 835, 1551, 1572, 1639
Esp3I CGTCTC 1 cut(s) 280
FaqI GGGAC 2 cut(s) 314, 1601
FauI CCCGC 1 cut(s) 1281
FbaI TGATCA 2 cut(s) 1153, 1537
FblI GTMKAC 2 cut(s) 1009, 2506
Fnu4HI GCNGC 9 cut(s) 233, 284, 311, 381, 452, 1409, 1867, 2066, 2497
FokI GGATG 5 cut(s) 372, 563, 1366, 1417, 1687
FriOI GRGCYC 1 cut(s) 153
Fsp4HI GCNGC 9 cut(s) 233, 284, 311, 381, 452, 1409, 1867, 2066, 2497
FspBI CTAG 4 cut(s) 653, 1245, 1893, 2192
GlaI GCGC 7 cut(s) 131, 172, 223, 225, 786, 1271, 2713
GluI GCNGC 9 cut(s) 233, 284, 311, 381, 452, 1409, 1867, 2066, 2497
GsaI CCCAGC 1 cut(s) 107
GsuI CTGGAG 3 cut(s) 50, 1408, 2616
HaeII RGCGCY 3 cut(s) 174, 788, 1273
HaeIII GGCC 3 cut(s) 140, 325, 537
HgaI GACGC 2 cut(s) 675, 1155
HhaI GCGC 7 cut(s) 132, 173, 224, 226, 787, 1272, 2714
Hin6I GCGC 7 cut(s) 130, 171, 222, 224, 785, 1270, 2712
HinP1I GCGC 7 cut(s) 130, 171, 222, 224, 785, 1270, 2712
HincII GTYRAC 3 cut(s) 619, 1420, 2308
HindII GTYRAC 3 cut(s) 619, 1420, 2308
HindIII AAGCTT 2 cut(s) 859, 1027
HpaI GTTAAC 1 cut(s) 2308
HphI GGTGA 6 cut(s) 694, 707, 1645, 2117, 2147, 2612
Hpy166II GTNNAC 9 cut(s) 124, 372, 619, 1010, 1333, 1420, 1512, 2308, 2507
Hpy8I GTNNAC 9 cut(s) 124, 372, 619, 1010, 1333, 1420, 1512, 2308, 2507
Hpy99I CGWCG 3 cut(s) 80, 379, 1791
HpyAV CCTTC 7 cut(s) 866, 1083, 1124, 1595, 1904, 2126, 2811
HpyCH4III ACNGT 3 cut(s) 1818, 1886, 2776
HpyCH4IV ACGT 3 cut(s) 120, 374, 1105
HpyF10VI GCNNNNNNNGC 4 cut(s) 219, 232, 636, 1568
HpySE526I ACGT 3 cut(s) 120, 374, 1105
HspAI GCGC 7 cut(s) 130, 171, 222, 224, 785, 1270, 2712
KflI GGGWCCC 1 cut(s) 301
KroI GCCGGC 4 cut(s) 62, 107, 167, 286
KroNI GCCGGC 4 cut(s) 64, 109, 169, 288
Ksp22I TGATCA 2 cut(s) 1153, 1537
KspAI GTTAAC 1 cut(s) 2308
LguI GCTCTTC 1 cut(s) 2413
LmnI GCTCC 7 cut(s) 178, 258, 295, 349, 1134, 1267, 1321
Lsp1109I GCAGC 6 cut(s) 244, 270, 1420, 1853, 2052, 2483
LweI GCATC 5 cut(s) 350, 433, 1060, 1395, 1853
MaeI CTAG 4 cut(s) 653, 1245, 1893, 2192
MaeII ACGT 3 cut(s) 120, 374, 1105
MflI RGATCY 2 cut(s) 1957, 2384
MhlI GDGCHC 2 cut(s) 153, 1139
MlyI GAGTC 5 cut(s) 563, 1169, 1838, 1898, 2296
MmeI TCCRAC 4 cut(s) 228, 849, 1287, 2005
MreI CGCCGGCG 1 cut(s) 167
MroNI GCCGGC 4 cut(s) 62, 107, 167, 286
MseI TTAA 7 cut(s) 267, 483, 644, 717, 2307, 2727, 2771
MslI CAYNNNNRTG 4 cut(s) 1144, 2079, 2097, 2103
MspA1I CMGCKG 1 cut(s) 313
MspCI CTTAAG 1 cut(s) 266
MspR9I CCNGG 6 cut(s) 29, 294, 539, 1748, 1749, 2088
Mva1269I GAATGC 1 cut(s) 1564
MvaI CCWGG 3 cut(s) 29, 539, 2088
MvnI CGCG 4 cut(s) 130, 222, 224, 357
MwoI GCNNNNNNNGC 4 cut(s) 219, 232, 636, 1568
NaeI GCCGGC 4 cut(s) 64, 109, 169, 288
NciI CCSGG 3 cut(s) 294, 1748, 1749
NcoI CCATGG 1 cut(s) 1639
NgoMIV GCCGGC 4 cut(s) 62, 107, 167, 286
NlaIV GGNNCC 7 cut(s) 254, 291, 302, 303, 503, 1549, 1992
NmeAIII GCCGAG 1 cut(s) 189
NspI RCATGY 3 cut(s) 458, 1526, 2078
PaeI GCATGC 1 cut(s) 1526
PaeR7I CTCGAG 4 cut(s) 134, 152, 227, 395
PagI TCATGA 1 cut(s) 2098
PauI GCGCGC 1 cut(s) 222
PceI AGGCCT 1 cut(s) 140
PciSI GCTCTTC 1 cut(s) 2413
PcsI WCGNNNNNNNCGW 3 cut(s) 314, 323, 380
PctI GAATGC 1 cut(s) 1564
PdiI GCCGGC 4 cut(s) 64, 109, 169, 288
PfeI GAWTC 8 cut(s) 244, 877, 1183, 1325, 1484, 1724, 2519, 2783
PflMI CCANNNNNTGG 2 cut(s) 49, 2341
PinAI ACCGGT 1 cut(s) 504
PkrI GCNGC 9 cut(s) 234, 285, 312, 382, 453, 1410, 1868, 2067, 2498
PleI GAGTC 5 cut(s) 562, 1168, 1837, 1897, 2295
PpsI GAGTC 5 cut(s) 562, 1168, 1837, 1897, 2295
PpuMI RGGWCCY 1 cut(s) 301
Psp124BI GAGCTC 1 cut(s) 153
Psp5II RGGWCCY 1 cut(s) 301
Psp6I CCWGG 3 cut(s) 27, 537, 2086
PspEI GGTNACC 1 cut(s) 700
PspFI CCCAGC 1 cut(s) 103
PspGI CCWGG 3 cut(s) 27, 537, 2086
PspN4I GGNNCC 7 cut(s) 254, 291, 302, 303, 503, 1549, 1992
PspPI GGNCC 2 cut(s) 40, 301
PspPPI RGGWCCY 1 cut(s) 301
PspXI VCTCGAGB 3 cut(s) 134, 227, 395
PstI CTGCAG 2 cut(s) 1828, 1871
PstNI CAGNNNCTG 1 cut(s) 2065
PsuI RGATCY 2 cut(s) 1957, 2384
PteI GCGCGC 1 cut(s) 222
RsaI GTAC 3 cut(s) 1471, 2489, 2593
RsaNI GTAC 3 cut(s) 1470, 2488, 2592
RseI CAYNNNNRTG 4 cut(s) 1144, 2079, 2097, 2103
SacI GAGCTC 1 cut(s) 153
SapI GCTCTTC 1 cut(s) 2413
SaqAI TTAA 7 cut(s) 267, 483, 644, 717, 2307, 2727, 2771
SatI GCNGC 9 cut(s) 233, 284, 311, 381, 452, 1409, 1867, 2066, 2497
Sau96I GGNCC 2 cut(s) 40, 301
SchI GAGTC 5 cut(s) 563, 1169, 1838, 1898, 2296
ScrFI CCNGG 6 cut(s) 29, 294, 539, 1748, 1749, 2088
SduI GDGCHC 2 cut(s) 153, 1139
SfaNI GCATC 5 cut(s) 350, 433, 1060, 1395, 1853
SfcI CTRYAG 3 cut(s) 1707, 1824, 1867
Sfr274I CTCGAG 4 cut(s) 134, 152, 227, 395
SgrAI CRCCGGYG 2 cut(s) 167, 493
SinI GGWCC 2 cut(s) 40, 301
SlaI CTCGAG 4 cut(s) 134, 152, 227, 395
SmaI CCCGGG 1 cut(s) 1749
SmiMI CAYNNNNRTG 4 cut(s) 1144, 2079, 2097, 2103
SmlI CTYRAG 6 cut(s) 134, 152, 227, 266, 395, 2510
SmoI CTYRAG 6 cut(s) 134, 152, 227, 266, 395, 2510
SphI GCATGC 1 cut(s) 1526
SseBI AGGCCT 1 cut(s) 140
SsiI CCGC 6 cut(s) 220, 311, 357, 380, 451, 1288
SspI AATATT 1 cut(s) 805
SspMI CTAG 4 cut(s) 653, 1245, 1893, 2192
SstI GAGCTC 1 cut(s) 153
StuI AGGCCT 1 cut(s) 140
StyD4I CCNGG 6 cut(s) 27, 292, 537, 1746, 1747, 2086
StyI CCWWGG 6 cut(s) 411, 658, 835, 1551, 1572, 1639
TaaI ACNGT 3 cut(s) 1818, 1886, 2776
TaiI ACGT 3 cut(s) 123, 377, 1108
TaqII GACCGA 1 cut(s) 894
TatI WGTACW 2 cut(s) 1469, 2487
TauI GCSGC 3 cut(s) 313, 383, 454
TfiI GAWTC 8 cut(s) 244, 877, 1183, 1325, 1484, 1724, 2519, 2783
Tru1I TTAA 7 cut(s) 267, 483, 644, 717, 2307, 2727, 2771
Tru9I TTAA 7 cut(s) 267, 483, 644, 717, 2307, 2727, 2771
TscAI CASTG 8 cut(s) 445, 1365, 1519, 1845, 1876, 1889, 2086, 2566
TseI GCWGC 6 cut(s) 232, 283, 1408, 1866, 2065, 2496
TspDTI ATGAA 9 cut(s) 738, 1233, 1274, 1614, 1772, 2142, 2331, 2436, 2487
TspMI CCCGGG 1 cut(s) 1747
TspRI CASTG 8 cut(s) 445, 1365, 1519, 1845, 1876, 1889, 2086, 2566
Van91I CCANNNNNTGG 2 cut(s) 49, 2341
Vha464I CTTAAG 1 cut(s) 266
VpaK11BI GGWCC 2 cut(s) 40, 301
XagI CCTNNNNNAGG 1 cut(s) 2001
XapI RAATTY 8 cut(s) 766, 1684, 1809, 2043, 2423, 2475, 2623, 2804
XceI RCATGY 3 cut(s) 458, 1526, 2078
XhoI CTCGAG 4 cut(s) 134, 152, 227, 395
XmaI CCCGGG 1 cut(s) 1747
XmiI GTMKAC 2 cut(s) 1009, 2506
XspI CTAG 4 cut(s) 653, 1245, 1893, 2192
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.