Rroxscaffold_3G00275090

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0001818
Physical Location & Seq
Forward (+)
66654198 .. 66659567
5370 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00275090.1

Sequence Viewer

Length: 3291 bp
ATGGCGATTGTGACCGGAGATCGGTACCTGGAGAAGCTAGTCCACTTCGTGGAGCAGCAAGCCGGTTCTCTGATCGACGGTTCGTTAGTGCTGAAGCTCAACCCGGCCGGCTTCCACTACGTCCACTCCCGCCTCGAGGCCTTGCACGAGCTCGAGAGCCTCCTCGCCGGCGCTCCCGTCGACTATCTCCGAGCCTATGTCTCCGACCTCGGCGACCACCGCGCCCTCGAGCAGCTCAGGAGGATTCTCAGGCTGTTGACGTCGCTTAAGGTCGTTTCGGTGCTGCCGGCTCCGGCCAGAGACCCCACGCCGCTGTCGTTTTGGCCGTTCGGGAGGCTTAGGGTTTTGGAGCTTCGGGGATGCGATTTGTCCACGTCGGCCGCGAAAGGTCTTCTCGAGTTGAGGCACACCTTGGAGAAGATCATCTGTCACAATTCAACTGATGCTCTGCGCCATGTGTTTGCGAGTAGAATTGCAGAGATAAAGGACTCGCCACTATGGCACCGGTTGTCATTTGTTTCGTGTGCTTGTAATGGCTTGGTTCTGATGGATGAGTCTTTGCAACTACTTCCGGCAGTTGAAACTCTTGACCTGAGCCGAAACAAGTTTGCAAAGATTGACAATCTTCGCAAGTGTGGCAAATTGAAACACCTAGACCTTGGTTTCAATCATCTGAGAACGATTTCGTCATTTGGTGAGGTCACCTCTCGACTACTTAAACTTGTTTTGAGGAACAATGCTCTAACTACGTTGCGTGGGATTGAGACTTTGAAGTCCCTTGAAGGGCTTGATGTTTCCTACAATATTATTTCCAATTTTTCAGAGTTAGAGTTCCTTGGTGGTCTTCCATCTCTACAAAGCTTGTGGCTGGAAGGGAATCCATTATGTTGTGCCAGTTGGTATCGGCCACAAGTATTCAGCTATTTCTATAATCCAGAAAAATTGAAGTTAGATGATAAGGAAATCAGCACCAGAGAGTTTTGGAAGAGGCAACTAATTATTGCAAGTAGACAGAAGCGACCTGCCAGCTTTGGGTTTTATTCTCCCATAAAGTGTGATGATAAAGGAGATGCAAGTATCAACAGAAGAAGGAAAAAGGTGTCTCGTCTTGCTTCCATTGTAAGTGAAGAAGGGAGCACATCTCTCTGTTCTGATCAGGAGTCTGTGTCTTGTGACAATGAGATTCAAAGCAGAGAAGAACTTGTTATATCAGATGAGGAAGCTGAAATTGTTGATTTGATGACTAGAGTTGAGCAAATGAAGAAAGAGCGCTCTGTTCTTTGGTTGCGAGAGTTCAAAGAATGGTTGGATCATGCTCCTGAGGATTCTGTGGACAGCAATAGACATAGCGGGACGACATTGCATATTGGCAGGGAAAATTATATAAAAGAAAAGTCAAGCTGGAGGCAGCTTGATAGTTCATTTATGGATATGTCTACTGGTTTGCATGCCCATCACCTTGATCAAACTGGTTCCTTGGGAAATGCTGGTTTTGTCATCCCAGTTGGCATGGATAGAAGAGATCTGAAGGAAACCCTAAAAGATAGTTCACGTGAAGGAACAAGTAGTGTCCCTTCACGAGCAAAAAATTTCCATGGTCACATGTTCACTGGTCAAAGAGGTCATAGGATGGTCGAAAATTTGAGTATGTCATTGACTTCTATAGATGATATTTCTGAATCTCGCTCCTCGTCTGTGTGCCCGGGATCACCACCTCATTATCAAAAGGACATTCTTAACCGTCGTCATAACTTGGAGGAAGAAATTTTACAGTTATCTGCAGAGTCATTTTCAGTGGCATCATCTGATAGTAATACCAGCTGTAGTGAAGACGATAACTATGAGTCTAGGCAGTCCATTCCTGAAGGTTATCAGCTACTGAATAAAAATATGGAAGAACATCCGTCTTCAGATCCTTTTAGGTTGTACGACATGAGATATGAGGTTCCACATGTAAGAGGAAATGACAGGTGTTCAGTTGGAATAGGGGCAGAGAAAATTTCAAATTCAGACCTATCGCTGCAATCACATGCCAGTGATGTTCCTGGTCGTGCTCATCATGCTGAGATTGCTCATTTTGTTGATGAAGAAGGTGATTTGTTAGAGAGGAGAAAACGCAGACAGAAAATAAAAAGAAGAGTTGTTACACTACTAGAAGATGACAAAATGATTAGGCAAGCAGAAACATTACCATCATTAAATGGTCATATAGAGAATCATGTAACAATACTGGAAGATGAGCAAGATAGTCGATCTTTCTATGGGATTAACTTTGATGAAGTTATTGGTAAGAACCAGATGTTGACAAATACAAGTAATATTCCACTAATAAATGATACTACTGGATCTTCTGGGGCTGAATGTTTCTCTTCAAAGAGTGATGAATTTATTGAGGATTATTTCAGAAAGAATGTTGCAGATATGGGAAATAATGAAATTTGCAAGCAATATATGCGCTGCTATTGTATACTTGAGTTGGATTCTCTATACAGAGAAAGAGAAGTAGCTGTCTTGCTGAGCAGTGAAAACAAAGTGTATGTGCTACTAATTGGTATTGGAGGTGATGGATCAGGCACCATCTTGAATTTGCAGGGTTCTCATAGGGTTGAAGACATAAGAGACGTCGTAGTTGGTGTAGGACTTCAGGTTGTGAGGGTGTATGTCGAGGAGGGTGCTACCTACCTCTTTAAAACCAGAAGCATTGAAAGTTCAAGACAGTTACTTTCAACTTTAACAGTTATTGATTCTTACTCTCCAATCGATAAATTTTGTCTGAGGAGTTTGGAGCAGGTTCAGGTTGGACTATTTGAGAAACAAATATGTGGAGGTTCAAAACTGAGCTTATTCCAGTATTCTATGGTGCAGTTTTGGTGTAGTAGTAGTGAAGATGGATCATGGCTTTCAAGATCAATATTTGTGGCTGGAGAGCATTTATTTGTGTGCTTTGAAGACCTCATGCAGTTTAGCTCTTTATCAGAGGATACGTCTCTACCACCATATTTTTCACTTGACTTGTGTTGCTCTATTGCTGACATATCTGAGCTGGTTGTCGAGACCAGGGAAAATCGGTGTGTAACCTTAGCTGTTGAGTGTGCAATGTCGGAATTCTGTTCTTCACCCAAAGCTGGGAAACAAGACCCAGCTGAAAATGACATAAAGACAGCTTCAGGCTCTATGACTTGGAAACTCAAGTGGTTTTCTGAAGAGAGTCCGTTCAAGTTTGTGGCATTGTTGAAGGCAATCCATGCCAGGTTGACTATTTCTCCATTGCTGATAAGATGTACATCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_4

Protein Analysis

1096

Amino Acids

123.66

Weight (kDa)

5.72

Isoelectric Point (pI)

55.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_4 PF12799 192 - 231 3.4e-06 Leucine Rich repeats (2 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 772
AatII GACGTC 2 cut(s) 263, 2625
AbsI CCTCGAGG 1 cut(s) 134
Acc36I ACCTGC 2 cut(s) 1030, 2779
Acc65I GGTACC 1 cut(s) 24
AccB1I GGYRCC 3 cut(s) 24, 501, 2573
AccB7I CCANNNNNTGG 1 cut(s) 49
AccI GTMKAC 4 cut(s) 180, 1009, 1436, 2467
AccII CGCG 2 cut(s) 222, 383
AciI CCGC 5 cut(s) 130, 220, 311, 381, 1350
AclWI GGATC 6 cut(s) 1317, 1714, 1907, 2353, 2575, 2899
AcoI YGGCCR 5 cut(s) 105, 294, 323, 378, 905
AcuI CTGAAG 7 cut(s) 113, 1547, 1884, 1893, 2627, 3150, 3222
AcvI CACGTG 1 cut(s) 1553
AcyI GRCGYC 2 cut(s) 260, 2622
AdeI CACNNNGTG 1 cut(s) 49
AfaI GTAC 3 cut(s) 26, 1928, 3283
AfeI AGCGCT 1 cut(s) 1271
AfiI CCNNNNNNNGG 7 cut(s) 21, 49, 136, 783, 1032, 3125, 3126
AflII CTTAAG 1 cut(s) 266
AflIII ACRYGT 2 cut(s) 1602, 1951
AgeI ACCGGT 1 cut(s) 504
AjiI CACGTC 1 cut(s) 375
AjnI CCWGG 4 cut(s) 27, 2044, 3056, 3248
Alw21I GWGCWC 3 cut(s) 153, 1139, 2056
Alw26I GTCTC 7 cut(s) 205, 294, 758, 1107, 2613, 2991, 3047
AlwI GGATC 6 cut(s) 1317, 1714, 1907, 2353, 2575, 2899
AlwNI CAGNNNCTG 1 cut(s) 1879
Ama87I CYCGRG 5 cut(s) 134, 152, 227, 395, 1702
Aor51HI AGCGCT 1 cut(s) 1271
AoxI GGCC 6 cut(s) 105, 138, 294, 323, 378, 905
ApeKI GCWGC 6 cut(s) 55, 232, 283, 1408, 2020, 2457
AsiGI ACCGGT 1 cut(s) 504
Asp700I GAANNNNTTC 1 cut(s) 682
Asp718I GGTACC 1 cut(s) 24
AspLEI GCGC 5 cut(s) 173, 224, 453, 1272, 2457
AsuC2I CCSGG 3 cut(s) 104, 1703, 1704
AsuHPI GGTGA 7 cut(s) 694, 707, 1448, 1701, 2105, 2573, 3108
AvaI CYCGRG 5 cut(s) 134, 152, 227, 395, 1702
AxyI CCTNAGG 1 cut(s) 1320
BaeGI GKGCMC 1 cut(s) 1703
BanI GGYRCC 3 cut(s) 24, 501, 2573
BanII GRGCYC 1 cut(s) 153
BarI GAAGNNNNNNTAC 6 cut(s) 1558, 1590, 1752, 1784, 2128, 2160
BauI CACGAG 2 cut(s) 146, 1578
BbrPI CACGTG 1 cut(s) 1553
BbsI GAAGAC 6 cut(s) 383, 836, 1836, 1899, 2616, 2955
Bbv12I GWGCWC 3 cut(s) 153, 1139, 2056
BbvI GCAGC 6 cut(s) 67, 244, 270, 1420, 2007, 2444
BccI CCATC 8 cut(s) 541, 856, 1461, 1624, 2200, 2558, 2585, 2882
BceAI ACGGC 1 cut(s) 310
BcgI CGANNNNNNTGC 4 cut(s) 2231, 2265, 2654, 2688
BciT130I CCWGG 4 cut(s) 29, 2046, 3058, 3250
BciVI GTATCC 1 cut(s) 2974
BclI TGATCA 2 cut(s) 1153, 1462
BcnI CCSGG 3 cut(s) 104, 1703, 1704
BcoDI GTCTC 7 cut(s) 205, 294, 758, 1107, 2613, 2991, 3047
BfaI CTAG 5 cut(s) 38, 653, 1245, 1848, 2153
BfmI CTRYAG 3 cut(s) 1662, 1779, 1822
BfoI RGCGCY 2 cut(s) 174, 1273
BfrI CTTAAG 1 cut(s) 266
BfuAI ACCTGC 2 cut(s) 1030, 2779
BfuI GTATCC 1 cut(s) 2974
BglI GCCNNNNNGGC 1 cut(s) 499
BglII AGATCT 1 cut(s) 1522
BisI GCNGC 8 cut(s) 56, 233, 284, 311, 381, 1409, 2021, 2458
BlpI GCTNAGC 1 cut(s) 2516
BlsI GCNGC 8 cut(s) 57, 234, 285, 312, 382, 1410, 2022, 2459
Bme1390I CCNGG 7 cut(s) 29, 104, 1703, 1704, 2046, 3058, 3250
BmeT110I CYCGRG 5 cut(s) 134, 152, 227, 395, 1702
BmgBI CACGTC 1 cut(s) 375
BmiI GGNNCC 6 cut(s) 26, 291, 503, 1474, 1947, 2575
BmrFI CCNGG 7 cut(s) 29, 104, 1703, 1704, 2046, 3058, 3250
BmrI ACTGGG 1 cut(s) 1496
BmsI GCATC 4 cut(s) 350, 433, 1060, 1808
BmuI ACTGGG 1 cut(s) 1496
BpiI GAAGAC 6 cut(s) 383, 836, 1836, 1899, 2616, 2955
BplI GAGNNNNNCTC 4 cut(s) 689, 721, 1126, 1158
BpmI CTGGAG 3 cut(s) 50, 1423, 2943
Bpu10I CCTNAGC 4 cut(s) 236, 338, 593, 3079
Bpu1102I GCTNAGC 1 cut(s) 2516
BpuEI CTTGAG 2 cut(s) 2492, 3173
BpuMI CCSGG 3 cut(s) 104, 1703, 1704
Bsa29I ATCGAT 1 cut(s) 2760
BsaAI YACGTR 1 cut(s) 1553
BsaBI GATNNNNATC 1 cut(s) 3283
BsaHI GRCGYC 2 cut(s) 260, 2622
BsaI GGTCTC 2 cut(s) 294, 3047
BsaJI CCNNGG 8 cut(s) 208, 411, 658, 835, 1476, 1594, 1702, 3057
BsaWI WCCGGW 2 cut(s) 14, 504
BsaXI ACNNNNNCTCC 6 cut(s) 110, 140, 1151, 1181, 3247, 3277
Bsc4I CCNNNNNNNGG 7 cut(s) 21, 49, 136, 783, 1032, 3125, 3126
Bse118I RCCGGY 5 cut(s) 62, 107, 167, 286, 504
Bse1I ACTGG 9 cut(s) 894, 1444, 1474, 1502, 1615, 2034, 2235, 2347, 2848
Bse21I CCTNAGG 1 cut(s) 1320
Bse3DI GCAATG 3 cut(s) 1358, 3102, 3266
Bse8I GATNNNNATC 1 cut(s) 3283
BseBI CCWGG 4 cut(s) 29, 2046, 3058, 3250
BseCI ATCGAT 1 cut(s) 2760
BseDI CCNNGG 8 cut(s) 208, 411, 658, 835, 1476, 1594, 1702, 3057
BseGI GGATG 5 cut(s) 365, 556, 1497, 1635, 1900
BseJI GATNNNNATC 1 cut(s) 3283
BseLI CCNNNNNNNGG 7 cut(s) 21, 49, 136, 783, 1032, 3125, 3126
BseMI GCAATG 3 cut(s) 1358, 3102, 3266
BseNI ACTGG 9 cut(s) 894, 1444, 1474, 1502, 1615, 2034, 2235, 2347, 2848
BseRI GAGGAG 5 cut(s) 152, 1678, 2122, 2681, 2791
BseSI GKGCMC 1 cut(s) 1703
BseX3I CGGCCG 2 cut(s) 105, 378
BseXI GCAGC 6 cut(s) 67, 244, 270, 1420, 2007, 2444
BseYI CCCAGC 2 cut(s) 3125, 3139
BsgI GTGCAG 1 cut(s) 2882
Bsh1236I CGCG 2 cut(s) 222, 383
Bsh1285I CGRYCG 2 cut(s) 108, 381
BshFI GGCC 6 cut(s) 107, 140, 296, 325, 380, 907
BshNI GGYRCC 3 cut(s) 24, 501, 2573
BshTI ACCGGT 1 cut(s) 504
BshVI ATCGAT 1 cut(s) 2760
BsiEI CGRYCG 2 cut(s) 108, 381
BsiHKAI GWGCWC 3 cut(s) 153, 1139, 2056
BsiHKCI CYCGRG 5 cut(s) 134, 152, 227, 395, 1702
BslFI GGGAC 3 cut(s) 760, 1366, 1556
BslI CCNNNNNNNGG 7 cut(s) 21, 49, 136, 783, 1032, 3125, 3126
BsmAI GTCTC 7 cut(s) 205, 294, 758, 1107, 2613, 2991, 3047
BsmBI CGTCTC 2 cut(s) 2613, 2991
BsmFI GGGAC 3 cut(s) 760, 1366, 1556
BsnI GGCC 6 cut(s) 107, 140, 296, 325, 380, 907
Bso31I GGTCTC 2 cut(s) 294, 3047
BsoBI CYCGRG 5 cut(s) 134, 152, 227, 395, 1702
Bsp1286I GDGCHC 4 cut(s) 153, 1139, 1703, 2056
Bsp1407I TGTACA 1 cut(s) 3281
Bsp1720I GCTNAGC 1 cut(s) 2516
Bsp19I CCATGG 1 cut(s) 1594
BspACI CCGC 5 cut(s) 130, 220, 311, 381, 1350
BspANI GGCC 6 cut(s) 107, 140, 296, 325, 380, 907
BspDI ATCGAT 1 cut(s) 2760
BspFNI CGCG 2 cut(s) 222, 383
BspHI TCATGA 1 cut(s) 3287
BspLI GGNNCC 6 cut(s) 26, 291, 503, 1474, 1947, 2575
BspMAI CTGCAG 1 cut(s) 1783
BspMI ACCTGC 2 cut(s) 1030, 2779
BspPI GGATC 6 cut(s) 1317, 1714, 1907, 2353, 2575, 2899
BspT107I GGYRCC 3 cut(s) 24, 501, 2573
BspTI CTTAAG 1 cut(s) 266
BspTNI GGTCTC 2 cut(s) 294, 3047
BsrDI GCAATG 3 cut(s) 1358, 3102, 3266
BsrFI RCCGGY 5 cut(s) 62, 107, 167, 286, 504
BsrGI TGTACA 1 cut(s) 3281
BsrI ACTGG 9 cut(s) 894, 1444, 1474, 1502, 1615, 2034, 2235, 2347, 2848
BssAI RCCGGY 5 cut(s) 62, 107, 167, 286, 504
BssECI CCNNGG 8 cut(s) 208, 411, 658, 835, 1476, 1594, 1702, 3057
BssNAI GTATAC 1 cut(s) 2468
BssNI GRCGYC 2 cut(s) 260, 2622
BssSI CACGAG 2 cut(s) 146, 1578
BssT1I CCWWGG 5 cut(s) 411, 658, 835, 1476, 1594
Bst1107I GTATAC 1 cut(s) 2468
Bst2BI CACGAG 2 cut(s) 146, 1578
Bst2UI CCWGG 4 cut(s) 29, 2046, 3058, 3250
Bst4CI ACNGT 5 cut(s) 80, 1742, 1773, 2718, 2737
Bst6I CTCTTC 5 cut(s) 980, 1513, 2131, 2374, 3198
BstACI GRCGYC 2 cut(s) 260, 2622
BstAFI CTTAAG 1 cut(s) 266
BstAPI GCANNNNNTGC 2 cut(s) 2452, 3245
BstAUI TGTACA 1 cut(s) 3281
BstBAI YACGTR 1 cut(s) 1553
BstC8I GCNNGC 8 cut(s) 60, 109, 169, 288, 1027, 1449, 2178, 2444
BstDSI CCRYGG 1 cut(s) 1594
BstEII GGTNACC 1 cut(s) 700
BstF5I GGATG 5 cut(s) 365, 556, 1497, 1635, 1900
BstFNI CGCG 2 cut(s) 222, 383
BstH2I RGCGCY 2 cut(s) 174, 1273
BstHHI GCGC 5 cut(s) 173, 224, 453, 1272, 2457
BstMAI GTCTC 7 cut(s) 205, 294, 758, 1107, 2613, 2991, 3047
BstMCI CGRYCG 2 cut(s) 108, 381
BstMWI GCNNNNNNNGC 7 cut(s) 219, 499, 636, 2060, 2069, 2452, 3245
BstNI CCWGG 4 cut(s) 29, 2046, 3058, 3250
BstNSI RCATGY 4 cut(s) 1451, 1606, 1955, 2033
BstPI GGTNACC 1 cut(s) 700
BstSCI CCNGG 7 cut(s) 27, 102, 1701, 1702, 2044, 3056, 3248
BstSFI CTRYAG 3 cut(s) 1662, 1779, 1822
BstSLI GKGCMC 1 cut(s) 1703
BstUI CGCG 2 cut(s) 222, 383
BstV1I GCAGC 6 cut(s) 67, 244, 270, 1420, 2007, 2444
BstV2I GAAGAC 6 cut(s) 383, 836, 1836, 1899, 2616, 2955
BstX2I RGATCY 3 cut(s) 1522, 1912, 2345
BstYI RGATCY 3 cut(s) 1522, 1912, 2345
BstZ17I GTATAC 1 cut(s) 2468
BstZI CGGCCG 2 cut(s) 105, 378
Bsu15I ATCGAT 1 cut(s) 2760
Bsu36I CCTNAGG 1 cut(s) 1320
BsuI GTATCC 1 cut(s) 2974
BsuRI GGCC 6 cut(s) 107, 140, 296, 325, 380, 907
BsuTUI ATCGAT 1 cut(s) 2760
BtgI CCRYGG 1 cut(s) 1594
BtrI CACGTC 1 cut(s) 375
BtsCI GGATG 5 cut(s) 365, 556, 1497, 1635, 1900
BtsI GCAGTG 1 cut(s) 2527
BtsIMutI CAGTG 4 cut(s) 1608, 1800, 2041, 2527
BveI ACCTGC 2 cut(s) 1030, 2779
Cac8I GCNNGC 8 cut(s) 60, 109, 169, 288, 1027, 1449, 2178, 2444
CaiI CAGNNNCTG 1 cut(s) 1879
CciI TCATGA 1 cut(s) 3287
CfoI GCGC 5 cut(s) 173, 224, 453, 1272, 2457
Cfr10I RCCGGY 5 cut(s) 62, 107, 167, 286, 504
Cfr9I CCCGGG 1 cut(s) 1702
ClaI ATCGAT 1 cut(s) 2760
Csp6I GTAC 3 cut(s) 25, 1927, 3282
CspAI ACCGGT 1 cut(s) 504
CspCI CAANNNNNGTGG 4 cut(s) 845, 880, 2898, 2933
CviQI GTAC 3 cut(s) 25, 1927, 3282
DraI TTTAAA 1 cut(s) 2689
DraIII CACNNNGTG 1 cut(s) 49
DrdI GACNNNNNNGTC 1 cut(s) 772
DseDI GACNNNNNNGTC 1 cut(s) 772
EaeI YGGCCR 5 cut(s) 105, 294, 323, 378, 905
EagI CGGCCG 2 cut(s) 105, 378
Eam1104I CTCTTC 5 cut(s) 980, 1513, 2131, 2374, 3198
EarI CTCTTC 5 cut(s) 980, 1513, 2131, 2374, 3198
Ecl136II GAGCTC 1 cut(s) 151
EclXI CGGCCG 2 cut(s) 105, 378
Eco130I CCWWGG 5 cut(s) 411, 658, 835, 1476, 1594
Eco147I AGGCCT 1 cut(s) 140
Eco24I GRGCYC 1 cut(s) 153
Eco31I GGTCTC 2 cut(s) 294, 3047
Eco47III AGCGCT 1 cut(s) 1271
Eco52I CGGCCG 2 cut(s) 105, 378
Eco53kI GAGCTC 1 cut(s) 151
Eco57I CTGAAG 7 cut(s) 113, 1547, 1884, 1893, 2627, 3150, 3222
Eco72I CACGTG 1 cut(s) 1553
Eco81I CCTNAGG 1 cut(s) 1320
Eco88I CYCGRG 5 cut(s) 134, 152, 227, 395, 1702
Eco91I GGTNACC 1 cut(s) 700
EcoICRI GAGCTC 1 cut(s) 151
EcoO65I GGTNACC 1 cut(s) 700
EcoRI GAATTC 1 cut(s) 3104
EcoRII CCWGG 4 cut(s) 27, 2044, 3056, 3248
EcoT14I CCWWGG 5 cut(s) 411, 658, 835, 1476, 1594
EcoT38I GRGCYC 1 cut(s) 153
ErhI CCWWGG 5 cut(s) 411, 658, 835, 1476, 1594
Esp3I CGTCTC 2 cut(s) 2613, 2991
FaqI GGGAC 3 cut(s) 760, 1366, 1556
FauI CCCGC 2 cut(s) 137, 1343
FbaI TGATCA 2 cut(s) 1153, 1462
FblI GTMKAC 4 cut(s) 180, 1009, 1436, 2467
Fnu4HI GCNGC 8 cut(s) 56, 233, 284, 311, 381, 1409, 2021, 2458
FokI GGATG 5 cut(s) 372, 563, 1484, 1642, 1887
FriOI GRGCYC 1 cut(s) 153
Fsp4HI GCNGC 8 cut(s) 56, 233, 284, 311, 381, 1409, 2021, 2458
FspBI CTAG 5 cut(s) 38, 653, 1245, 1848, 2153
GlaI GCGC 5 cut(s) 172, 223, 452, 1271, 2456
GluI GCNGC 8 cut(s) 56, 233, 284, 311, 381, 1409, 2021, 2458
GsaI CCCAGC 2 cut(s) 3129, 3143
GsuI CTGGAG 3 cut(s) 50, 1423, 2943
HaeII RGCGCY 2 cut(s) 174, 1273
HaeIII GGCC 6 cut(s) 107, 140, 296, 325, 380, 907
HhaI GCGC 5 cut(s) 173, 224, 453, 1272, 2457
Hin1I GRCGYC 2 cut(s) 260, 2622
Hin6I GCGC 5 cut(s) 171, 222, 451, 1270, 2455
HinP1I GCGC 5 cut(s) 171, 222, 451, 1270, 2455
HincII GTYRAC 4 cut(s) 181, 258, 2304, 3255
HindII GTYRAC 4 cut(s) 181, 258, 2304, 3255
HindIII AAGCTT 1 cut(s) 859
HphI GGTGA 7 cut(s) 694, 707, 1448, 1701, 2105, 2573, 3108
Hpy99I CGWCG 6 cut(s) 80, 182, 265, 379, 1746, 2627
HpyCH4III ACNGT 5 cut(s) 80, 1742, 1773, 2718, 2737
HpyCH4IV ACGT 7 cut(s) 120, 260, 374, 749, 1552, 2622, 2984
HpyF10VI GCNNNNNNNGC 7 cut(s) 219, 499, 636, 2060, 2069, 2452, 3245
HpySE526I ACGT 7 cut(s) 120, 260, 374, 749, 1552, 2622, 2984
Hsp92I GRCGYC 2 cut(s) 260, 2622
HspAI GCGC 5 cut(s) 171, 222, 451, 1270, 2455
KpnI GGTACC 1 cut(s) 28
KroI GCCGGC 3 cut(s) 107, 167, 286
KroNI GCCGGC 3 cut(s) 109, 169, 288
Ksp22I TGATCA 2 cut(s) 1153, 1462
LmnI GCTCC 8 cut(s) 52, 178, 295, 349, 1134, 1321, 1691, 2785
Lsp1109I GCAGC 6 cut(s) 67, 244, 270, 1420, 2007, 2444
LweI GCATC 4 cut(s) 350, 433, 1060, 1808
MaeI CTAG 5 cut(s) 38, 653, 1245, 1848, 2153
MaeII ACGT 7 cut(s) 120, 260, 374, 749, 1552, 2622, 2984
MaeIII GTNAC 9 cut(s) 10, 428, 700, 1172, 1598, 2143, 2221, 2718, 3073
MflI RGATCY 3 cut(s) 1522, 1912, 2345
MhlI GDGCHC 4 cut(s) 153, 1139, 1703, 2056
MlyI GAGTC 6 cut(s) 482, 563, 1169, 1793, 1853, 3217
MmeI TCCRAC 6 cut(s) 228, 1287, 1960, 2457, 2779, 3081
MreI CGCCGGCG 1 cut(s) 167
MroNI GCCGGC 3 cut(s) 107, 167, 286
MroXI GAANNNNTTC 1 cut(s) 682
MseI TTAA 7 cut(s) 267, 717, 1737, 2198, 2268, 2688, 2732
MslI CAYNNNNRTG 1 cut(s) 2034
MspA1I CMGCKG 3 cut(s) 313, 1821, 3143
MspCI CTTAAG 1 cut(s) 266
MspR9I CCNGG 7 cut(s) 29, 104, 1703, 1704, 2046, 3058, 3250
MvaI CCWGG 4 cut(s) 29, 2046, 3058, 3250
MvnI CGCG 2 cut(s) 222, 383
MwoI GCNNNNNNNGC 7 cut(s) 219, 499, 636, 2060, 2069, 2452, 3245
NaeI GCCGGC 3 cut(s) 109, 169, 288
NciI CCSGG 3 cut(s) 104, 1703, 1704
NcoI CCATGG 1 cut(s) 1594
NgoMIV GCCGGC 3 cut(s) 107, 167, 286
NlaIV GGNNCC 6 cut(s) 26, 291, 503, 1474, 1947, 2575
NmeAIII GCCGAG 1 cut(s) 189
NmuCI GTSAC 5 cut(s) 10, 428, 700, 1172, 1598
NspI RCATGY 4 cut(s) 1451, 1606, 1955, 2033
PaeI GCATGC 1 cut(s) 1451
PaeR7I CTCGAG 4 cut(s) 134, 152, 227, 395
PagI TCATGA 1 cut(s) 3287
PceI AGGCCT 1 cut(s) 140
PciI ACATGT 2 cut(s) 1602, 1951
PcsI WCGNNNNNNNCGW 3 cut(s) 314, 323, 380
PdiI GCCGGC 3 cut(s) 109, 169, 288
PdmI GAANNNNTTC 1 cut(s) 682
PfeI GAWTC 8 cut(s) 244, 877, 1183, 1325, 1679, 2215, 2480, 2744
PflMI CCANNNNNTGG 1 cut(s) 49
PinAI ACCGGT 1 cut(s) 504
PkrI GCNGC 8 cut(s) 57, 234, 285, 312, 382, 1410, 2022, 2459
PleI GAGTC 6 cut(s) 482, 562, 1168, 1792, 1852, 3216
PmaCI CACGTG 1 cut(s) 1553
PmlI CACGTG 1 cut(s) 1553
PpsI GAGTC 6 cut(s) 482, 562, 1168, 1792, 1852, 3216
Ppu21I YACGTR 1 cut(s) 1553
PscI ACATGT 2 cut(s) 1602, 1951
Psp124BI GAGCTC 1 cut(s) 153
Psp6I CCWGG 4 cut(s) 27, 2044, 3056, 3248
PspCI CACGTG 1 cut(s) 1553
PspEI GGTNACC 1 cut(s) 700
PspFI CCCAGC 2 cut(s) 3125, 3139
PspGI CCWGG 4 cut(s) 27, 2044, 3056, 3248
PspN4I GGNNCC 6 cut(s) 26, 291, 503, 1474, 1947, 2575
PspXI VCTCGAGB 2 cut(s) 134, 227
PstI CTGCAG 1 cut(s) 1783
PstNI CAGNNNCTG 1 cut(s) 1879
PsuI RGATCY 3 cut(s) 1522, 1912, 2345
PvuII CAGCTG 2 cut(s) 1821, 3143
RsaI GTAC 3 cut(s) 26, 1928, 3283
RsaNI GTAC 3 cut(s) 25, 1927, 3282
RseI CAYNNNNRTG 1 cut(s) 2034
SacI GAGCTC 1 cut(s) 153
SalI GTCGAC 1 cut(s) 179
SaqAI TTAA 7 cut(s) 267, 717, 1737, 2198, 2268, 2688, 2732
SatI GCNGC 8 cut(s) 56, 233, 284, 311, 381, 1409, 2021, 2458
SchI GAGTC 6 cut(s) 482, 563, 1169, 1793, 1853, 3217
ScrFI CCNGG 7 cut(s) 29, 104, 1703, 1704, 2046, 3058, 3250
SduI GDGCHC 4 cut(s) 153, 1139, 1703, 2056
SfaNI GCATC 4 cut(s) 350, 433, 1060, 1808
SfcI CTRYAG 3 cut(s) 1662, 1779, 1822
Sfr274I CTCGAG 4 cut(s) 134, 152, 227, 395
SgrAI CRCCGGYG 1 cut(s) 167
SlaI CTCGAG 4 cut(s) 134, 152, 227, 395
SmaI CCCGGG 1 cut(s) 1704
SmiMI CAYNNNNRTG 1 cut(s) 2034
SmlI CTYRAG 7 cut(s) 134, 152, 227, 266, 395, 2471, 3188
SmoI CTYRAG 7 cut(s) 134, 152, 227, 266, 395, 2471, 3188
SphI GCATGC 1 cut(s) 1451
SseBI AGGCCT 1 cut(s) 140
SsiI CCGC 5 cut(s) 130, 220, 311, 381, 1350
SspI AATATT 3 cut(s) 805, 2320, 2913
SspMI CTAG 5 cut(s) 38, 653, 1245, 1848, 2153
SstI GAGCTC 1 cut(s) 153
StuI AGGCCT 1 cut(s) 140
StyD4I CCNGG 7 cut(s) 27, 102, 1701, 1702, 2044, 3056, 3248
StyI CCWWGG 5 cut(s) 411, 658, 835, 1476, 1594
TaaI ACNGT 5 cut(s) 80, 1742, 1773, 2718, 2737
TaiI ACGT 7 cut(s) 123, 263, 377, 752, 1555, 2625, 2987
TatI WGTACW 1 cut(s) 3281
TauI GCSGC 2 cut(s) 313, 383
TfiI GAWTC 8 cut(s) 244, 877, 1183, 1325, 1679, 2215, 2480, 2744
Tru1I TTAA 7 cut(s) 267, 717, 1737, 2198, 2268, 2688, 2732
Tru9I TTAA 7 cut(s) 267, 717, 1737, 2198, 2268, 2688, 2732
TscAI CASTG 4 cut(s) 1615, 1800, 2041, 2527
TseFI GTSAC 5 cut(s) 10, 428, 700, 1172, 1598
TseI GCWGC 6 cut(s) 55, 232, 283, 1408, 2020, 2457
Tsp45I GTSAC 5 cut(s) 10, 428, 700, 1172, 1598
TspDTI ATGAA 6 cut(s) 1274, 1410, 2100, 2292, 2397, 2448
TspGWI ACGGA 2 cut(s) 1893, 3201
TspMI CCCGGG 1 cut(s) 1702
TspRI CASTG 4 cut(s) 1615, 1800, 2041, 2527
Van91I CCANNNNNTGG 1 cut(s) 49
Vha464I CTTAAG 1 cut(s) 266
XceI RCATGY 4 cut(s) 1451, 1606, 1955, 2033
XhoI CTCGAG 4 cut(s) 134, 152, 227, 395
XmaI CCCGGG 1 cut(s) 1702
XmiI GTMKAC 4 cut(s) 180, 1009, 1436, 2467
XmnI GAANNNNTTC 1 cut(s) 682
XspI CTAG 5 cut(s) 38, 653, 1245, 1848, 2153
ZraI GACGTC 2 cut(s) 261, 2623
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.