MD14G1237500.v1.1

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0001818
Physical Location & Seq
Forward (+)
31710649 .. 31716237
5589 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1237500.v1.1.491

Sequence Viewer

Length: 3270 bp
ATGGCGGTTGTCACCGGAGATCGCTACCTGGAGAAGCTGGTCCACTTCGTGGAGCAGCAGGCCGGTTCGTTGATCGACGGCAGCCTAGTGCTGAAGCTGAACCCGGCCGGGTTCCACTACGTCAACTCGCGGCTCGAGGCCTTGCACGAGCTCGAGAGCCTCCTCGCCGGCGCTCCCGTCGACTACCTCCGTGCCTACGTCTCCGACCTCGGAGACCACCGCGCGCTTGAGCAGCTGAGGAGGATTCTCAGGCTCTTGACATCCCTCAAGGTCGTTTCGGTGCTGCCGTCTCCCGCCAGGGACCCTACGCCGCTGTCGTTTCGGCCGTTTGGGAGGCTTAGGGTTTTGGAGCTTCGAGGATGCGATTTGTCCACCTCCGCCGCAAAAGGGTTGCTCGAGCTGAGGCACACCTTGGAGAAGATCATTTGCCATAATTCTACTGATGCTCTGCGGCATGTGTTTGCTAGTAGAATTGCAGAGATTAAGGACTCACCGCAGTGGAATCGCTTGTCATTCGTTTCGTGTGCTTGTAATGGCTTGGTTCTCATGGATGAGTCTTTGCAACTCCTTCCTGCTGTCGAAACTCTGGACCTTAGCCGGAACAAGTTTGCAATTGTTGATAATCTTCGGAAGTGTGTCAAATTGAAGCACCTAGACCTTGGTTTCAATCAGCTCAGAACGATTTCGTCATTCAGTCAGGTCACCTGCCGTTTACTTAAACTTGTTTTGAGGAACAATGCTCTAACTACATTGCGTGGGATTGAGAATTTGAAGTCACTTGAAGGGCTTGATGTTTCCTACAATATTATTTCAAATTTTTCGGAGTTAGAATACCTTTCTGGACTTCCATCTCTTGAAAACTTGTGGTTGGAAGGGAATCCGTTGTGTTGTGCCCGTTGGTATCGTTCACAAGCATTCAGTTATGTCACTAATCCATACAAATTGAAGTTAGATGATAAGGAGATCAGCACCAGAGACTTTTGGAAGAGGCAACTTATTGTTGCTGGCCGGCACAAACGACCGGCCAGCTTTGGGTTTTATTCTCCTGCAAAGTGTGATGATAAAGGAGATTCAAGTACTAACAGAAAAAGGAAAAAGGTATCTCGTCTTGCTTCAATTGTTAATGAAGAGGAGAACACATGTTCTGACCAGGACTCTGTGTCTTGTGACAATGAGATTCAAAGCAGAGAGGAAGTTGTCATATCTGACAATGAAGCTGAAATTGTTGATTTGATGACTAGAGTTGAGCTAATGAAGAAAGAGCGCTCTGTTCTTTGGTTGCGGGAGTTCAAGGAGTGGTTAGATCATGCTTCTGAGAATGATGCAGACATTGGTAGGTATAGCAGGGCTAGACTGCATATCGAGAAGGAAAACTATTTAAAAACCAAGGCAAGCTGGAGGCAGCTGGGTGAAAAATTAACATATGTTCCAGACTACATCCAAGCATCAGGAGATGAGAGTAGTACAAATGTTTTAGAATCAGACAGTTCCTTCTTGGATATATCTTCTGGACATGCCCATCACTTTGACCAAATTGTTTCAACGGGGAATGCTGGTAGGGTCAGCCCAGTTGGCATGGATAGTAGAGACCTCAGGGAGAATTTAAAAGCCTATTCTCATGAAGGGACAAGTACTGTCAGTTCAGAGGCTAAAAGTTTCCGTGCTCAGACGTTCACCACTCAAGGAGGTCATAGGATGATCCAGAATTTGAGCATGTCTGCATTATCTGTTATTGATGATATTTCAGAGTCTTATTCATCATCTGCTAACCCAGGATCACCGCCCCATTATCAAAAGGACATTCTTAGTCGTAGACATAACTTAGAGGAGGAAATTTTACAGTTATCCGCAGAGTCATATTCAGTGGCATCATCTGATAGTAATACCAGCTGTAGTGAAGATGAAAATTATGAATCTACGCAGTCGGTTCCTGAGAACTGGTTAAATGAGAGTGCTGAAGAATACCCACTTTCAAATCGTTTCAAGTATTATGACATAAAACGTGAGGTTGACAGACGTTCGGTTGGAGTATCTGCCAGTGATGTTCCTGCTGATGCTCATGATGTTGAAATTAATAATTGCATTAATGAAGAGTGTGATTTTCTGGAGAGGAGAAAAGGAAGACAGAAGACTAAAAGAAGAGTTACTGCTCTACTAGCAGATGATAACATGATTAGGCAAGCAGAACGAGCACCAAAATCAAATGGCATTCTAGATAATCATGTAGCGCAAGTCGAAAATAAGCAAGATAATCAGTATTTTTATGGGAGTGGCTTTCATGAAGTTAATAAGAGACAGATGTTGGCAGATAGAAGCAGTACCCCACTAACTGAATTCTTATCTTCAGGCAATGATGAATTTATTGAGAATTATTTCAATACAAATGTCGCAGATTCACGAAATCACGAGATTTGTAAGCAATGTTTGTGTTGCTGTTGTATACTTGAGCTGGATTTTTTTTCCAGAGAAAGAGAAGTTGCTGTTGTACTTAGTAGTGAAGACAAGCTTTATATACTGCTCATCGGTGTTGTAGGAGATGGGTCAGGCACTATGTTAAAGTTAAAGGGTTGCCACAGGGTTGAGGATATCAGAGAAGTTGTAGTTGGTATAGGGCTCCAGGTCGTGAGGGTGTATGTTGGGGGCAGTGCGACTTACGTGTTTAAAACTAGAAGCATTGAAAAATCAAGACAACTACTTTCTACCTTAAAAGCTATCGATTCTTTTGCGCCAAATGGAGGACTTTGCTTAAGAAGTTTGGAGCAGGTTCAGGTGGAGTTATTTGAGAAACAAATATGCGGAGGTTCAAAAGTGAGCATATTCCAGTATTCTATGGTGCAGTTTTGGTGTAGTTATAATGAAGGTGAATCATGGTTTTCAAGATCATTATTTGTGGCAGGAGGGCACGTATTTTTGTGTTTTGAAGACCTTATGCAGTTCAGCTCTTTGTCCGTGGATGCGCCTTTGCCCTCATATTTTTCACTTGACTTACGTTGCTCTATTGCCGACATATCTGAGTTGGTTGTTGATGTCAGAGAAAGTCGGTGTGTAACCTTAGCTGTTGAGTGTGCAATGTCCCAGTTCTGCCCCTCAGGCTCAGCCGTAGCGGACAATTCAGACTCAAGTGCAAATGAGAAAAAGATAGCTCCAGGCTCCATGACTTGGAAGCTCCGGTGGTTTTCCGAAGAGAGTCCGTTCAAGTTTGTGGCATTGTTGAAGGCGATCCACGGAGGGATGACCGTTACCCCTTTGCTTGTAAGGTGTATATCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_4

Protein Analysis

1090

Amino Acids

122.48

Weight (kDa)

5.79

Isoelectric Point (pI)

51.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_4 PF12799 192 - 231 1.1e-06 Leucine Rich repeats (2 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2853
AarI CACCTGC 1 cut(s) 713
Acc36I ACCTGC 2 cut(s) 713, 2752
AccB7I CCANNNNNTGG 2 cut(s) 49, 3159
AccI GTMKAC 3 cut(s) 180, 1813, 2440
AccII CGCG 3 cut(s) 130, 222, 224
AclWI GGATC 3 cut(s) 1693, 1783, 3214
AcoI YGGCCR 4 cut(s) 105, 323, 1006, 1023
AcsI RAATTY 7 cut(s) 766, 814, 1600, 1705, 1833, 2333, 2357
AcuI CTGAAG 3 cut(s) 113, 1977, 2328
AdeI CACNNNGTG 1 cut(s) 49
AfaI GTAC 5 cut(s) 1078, 1465, 1633, 2320, 2487
AfeI AGCGCT 1 cut(s) 1265
AfiI CCNNNNNNNGG 5 cut(s) 49, 387, 1032, 2735, 3159
AflII CTTAAG 1 cut(s) 2746
AflIII ACRYGT 2 cut(s) 1139, 2655
AhdI GACNNNNNGTC 1 cut(s) 1159
AjnI CCWGG 6 cut(s) 27, 296, 1149, 1771, 2616, 3145
AjuI GAANNNNNNNTTGG 2 cut(s) 2586, 2618
AleI CACNNNNGTG 1 cut(s) 496
Alw21I GWGCWC 3 cut(s) 153, 1666, 2194
Alw26I GTCTC 6 cut(s) 205, 207, 294, 969, 1581, 2287
AlwI GGATC 3 cut(s) 1693, 1783, 3214
Ama87I CYCGRG 3 cut(s) 134, 152, 395
Aor51HI AGCGCT 1 cut(s) 1265
AoxI GGCC 6 cut(s) 60, 105, 138, 323, 1006, 1023
ApeKI GCWGC 5 cut(s) 55, 81, 232, 283, 1402
ApoI RAATTY 7 cut(s) 766, 814, 1600, 1705, 1833, 2333, 2357
AseI ATTAAT 2 cut(s) 2073, 2085
Asp700I GAANNNNTTC 2 cut(s) 682, 2372
AspLEI GCGC 7 cut(s) 173, 224, 226, 1266, 2230, 2728, 2959
AspS9I GGNCC 3 cut(s) 40, 301, 589
AsuC2I CCSGG 2 cut(s) 104, 109
AsuHPI GGTGA 7 cut(s) 4, 483, 694, 1421, 1666, 1770, 2873
AvaI CYCGRG 3 cut(s) 134, 152, 395
AvaII GGWCC 3 cut(s) 40, 301, 589
AxyI CCTNAGG 2 cut(s) 1592, 3088
BaeGI GKGCMC 2 cut(s) 895, 2904
BanII GRGCYC 2 cut(s) 153, 2616
BauI CACGAG 2 cut(s) 146, 2405
BbsI GAAGAC 4 cut(s) 2128, 2135, 2505, 2928
Bbv12I GWGCWC 3 cut(s) 153, 1666, 2194
BbvCI CCTCAGC 2 cut(s) 236, 401
BbvI GCAGC 5 cut(s) 67, 93, 244, 270, 1414
BccI CCATC 3 cut(s) 856, 1527, 2531
BceAI ACGGC 5 cut(s) 94, 271, 310, 693, 3083
BcgI CGANNNNNNTGC 4 cut(s) 485, 519, 2705, 2739
BciT130I CCWGG 6 cut(s) 29, 298, 1151, 1773, 2618, 3147
BcnI CCSGG 2 cut(s) 104, 109
BcoDI GTCTC 6 cut(s) 205, 207, 294, 969, 1581, 2287
BfaI CTAG 8 cut(s) 86, 465, 653, 1239, 1350, 2156, 2213, 2667
BfmI CTRYAG 1 cut(s) 1891
BfoI RGCGCY 2 cut(s) 174, 1267
BfrI CTTAAG 1 cut(s) 2746
BfuAI ACCTGC 2 cut(s) 713, 2752
BglI GCCNNNNNGGC 2 cut(s) 1572, 3090
BisI GCNGC 9 cut(s) 56, 82, 131, 233, 284, 311, 381, 452, 1403
BlpI GCTNAGC 1 cut(s) 3094
BlsI GCNGC 9 cut(s) 57, 83, 132, 234, 285, 312, 382, 453, 1404
BmcAI AGTACT 2 cut(s) 1078, 1633
Bme1390I CCNGG 8 cut(s) 29, 104, 109, 298, 1151, 1773, 2618, 3147
Bme18I GGWCC 3 cut(s) 40, 301, 589
BmeRI GACNNNNNGTC 1 cut(s) 1159
BmeT110I CYCGRG 3 cut(s) 134, 152, 395
BmgT120I GGNCC 3 cut(s) 40, 301, 589
BmiI GGNNCC 6 cut(s) 113, 302, 303, 1929, 2615, 3151
BmrFI CCNGG 8 cut(s) 29, 104, 109, 298, 1151, 1773, 2618, 3147
BmrI ACTGGG 2 cut(s) 1562, 3070
BmsI GCATC 7 cut(s) 350, 433, 1312, 1454, 1877, 2044, 2944
BmuI ACTGGG 2 cut(s) 1562, 3070
BpiI GAAGAC 4 cut(s) 2128, 2135, 2505, 2928
BpmI CTGGAG 5 cut(s) 50, 1417, 2126, 2600, 3129
Bpu10I CCTNAGC 5 cut(s) 236, 338, 401, 593, 3052
Bpu1102I GCTNAGC 1 cut(s) 3094
BpuEI CTTGAG 5 cut(s) 248, 251, 1665, 2465, 3103
BpuMI CCSGG 2 cut(s) 104, 109
Bsa29I ATCGAT 1 cut(s) 2715
BsaAI YACGTR 2 cut(s) 2656, 2905
BsaI GGTCTC 2 cut(s) 207, 1581
BsaJI CCNNGG 8 cut(s) 208, 297, 411, 658, 1386, 1771, 2949, 3223
BsaWI WCCGGW 2 cut(s) 14, 3168
BsaXI ACNNNNNCTCC 2 cut(s) 2889, 2919
Bsc4I CCNNNNNNNGG 5 cut(s) 49, 387, 1032, 2735, 3159
Bse118I RCCGGY 4 cut(s) 62, 167, 1008, 1021
Bse1I ACTGG 5 cut(s) 1568, 1943, 2037, 2821, 3076
Bse21I CCTNAGG 2 cut(s) 1592, 3088
Bse3DI GCAATG 4 cut(s) 749, 2356, 2426, 3075
BseBI CCWGG 6 cut(s) 29, 298, 1151, 1773, 2618, 3147
BseCI ATCGAT 1 cut(s) 2715
BseDI CCNNGG 8 cut(s) 208, 297, 411, 658, 1386, 1771, 2949, 3223
BseGI GGATG 7 cut(s) 260, 365, 556, 1437, 1701, 2959, 3237
BseLI CCNNNNNNNGG 5 cut(s) 49, 387, 1032, 2735, 3159
BseMI GCAATG 4 cut(s) 749, 2356, 2426, 3075
BseNI ACTGG 5 cut(s) 1568, 1943, 2037, 2821, 3076
BsePI GCGCGC 1 cut(s) 222
BseRI GAGGAG 5 cut(s) 152, 253, 1145, 1841, 2125
BseSI GKGCMC 2 cut(s) 895, 2904
BseX3I CGGCCG 2 cut(s) 105, 323
BseXI GCAGC 5 cut(s) 67, 93, 244, 270, 1414
BseYI CCCAGC 1 cut(s) 1405
BsgI GTGCAG 1 cut(s) 2855
Bsh1236I CGCG 3 cut(s) 130, 222, 224
Bsh1285I CGRYCG 3 cut(s) 108, 326, 1022
BshFI GGCC 6 cut(s) 62, 107, 140, 325, 1008, 1025
BshVI ATCGAT 1 cut(s) 2715
BsiEI CGRYCG 3 cut(s) 108, 326, 1022
BsiHKAI GWGCWC 3 cut(s) 153, 1666, 2194
BsiHKCI CYCGRG 3 cut(s) 134, 152, 395
BsiSI CCGG 9 cut(s) 15, 63, 104, 108, 168, 598, 1009, 1022, 3169
BslFI GGGAC 3 cut(s) 314, 1639, 3058
BslI CCNNNNNNNGG 5 cut(s) 49, 387, 1032, 2735, 3159
BsmAI GTCTC 6 cut(s) 205, 207, 294, 969, 1581, 2287
BsmBI CGTCTC 2 cut(s) 205, 294
BsmFI GGGAC 3 cut(s) 314, 1639, 3058
BsmI GAATGC 3 cut(s) 914, 1555, 2208
BsnI GGCC 6 cut(s) 62, 107, 140, 325, 1008, 1025
Bso31I GGTCTC 2 cut(s) 207, 1581
BsoBI CYCGRG 3 cut(s) 134, 152, 395
Bsp1286I GDGCHC 6 cut(s) 153, 895, 1666, 2194, 2616, 2904
Bsp143I GATC 9 cut(s) 19, 72, 420, 963, 1303, 1698, 1775, 2879, 3219
Bsp1720I GCTNAGC 1 cut(s) 3094
BspANI GGCC 6 cut(s) 62, 107, 140, 325, 1008, 1025
BspDI ATCGAT 1 cut(s) 2715
BspFNI CGCG 3 cut(s) 130, 222, 224
BspHI TCATGA 3 cut(s) 1618, 2059, 2278
BspLI GGNNCC 6 cut(s) 113, 302, 303, 1929, 2615, 3151
BspMI ACCTGC 2 cut(s) 713, 2752
BspPI GGATC 3 cut(s) 1693, 1783, 3214
BspTI CTTAAG 1 cut(s) 2746
BspTNI GGTCTC 2 cut(s) 207, 1581
BsrDI GCAATG 4 cut(s) 749, 2356, 2426, 3075
BsrFI RCCGGY 4 cut(s) 62, 167, 1008, 1021
BsrI ACTGG 5 cut(s) 1568, 1943, 2037, 2821, 3076
BssAI RCCGGY 4 cut(s) 62, 167, 1008, 1021
BssECI CCNNGG 8 cut(s) 208, 297, 411, 658, 1386, 1771, 2949, 3223
BssHII GCGCGC 1 cut(s) 222
BssMI GATC 9 cut(s) 19, 72, 420, 963, 1303, 1698, 1775, 2879, 3219
BssNAI GTATAC 1 cut(s) 2441
BssSI CACGAG 2 cut(s) 146, 2405
BssT1I CCWWGG 3 cut(s) 411, 658, 1386
Bst1107I GTATAC 1 cut(s) 2441
Bst2BI CACGAG 2 cut(s) 146, 2405
Bst2UI CCWGG 6 cut(s) 29, 298, 1151, 1773, 2618, 3147
Bst4CI ACNGT 4 cut(s) 1487, 1636, 1842, 3238
Bst6I CTCTTC 5 cut(s) 980, 1122, 2085, 2134, 3177
BstAFI CTTAAG 1 cut(s) 2746
BstBAI YACGTR 2 cut(s) 2656, 2905
BstC8I GCNNGC 8 cut(s) 60, 169, 224, 1006, 1010, 1027, 1393, 2181
BstDSI CCRYGG 2 cut(s) 2949, 3223
BstEII GGTNACC 1 cut(s) 700
BstF5I GGATG 7 cut(s) 260, 365, 556, 1437, 1701, 2959, 3237
BstFNI CGCG 3 cut(s) 130, 222, 224
BstH2I RGCGCY 2 cut(s) 174, 1267
BstHHI GCGC 7 cut(s) 173, 224, 226, 1266, 2230, 2728, 2959
BstKTI GATC 9 cut(s) 22, 75, 423, 966, 1306, 1701, 1778, 2882, 3222
BstMAI GTCTC 6 cut(s) 205, 207, 294, 969, 1581, 2287
BstMBI GATC 9 cut(s) 19, 72, 420, 963, 1303, 1698, 1775, 2879, 3219
BstMCI CGRYCG 3 cut(s) 108, 326, 1022
BstMWI GCNNNNNNNGC 5 cut(s) 232, 1572, 2156, 2189, 3090
BstNI CCWGG 6 cut(s) 29, 298, 1151, 1773, 2618, 3147
BstNSI RCATGY 4 cut(s) 458, 1143, 1517, 1717
BstPI GGTNACC 1 cut(s) 700
BstSCI CCNGG 8 cut(s) 27, 102, 107, 296, 1149, 1771, 2616, 3145
BstSFI CTRYAG 1 cut(s) 1891
BstSLI GKGCMC 2 cut(s) 895, 2904
BstUI CGCG 3 cut(s) 130, 222, 224
BstV1I GCAGC 5 cut(s) 67, 93, 244, 270, 1414
BstV2I GAAGAC 4 cut(s) 2128, 2135, 2505, 2928
BstZ17I GTATAC 1 cut(s) 2441
BstZI CGGCCG 2 cut(s) 105, 323
Bsu15I ATCGAT 1 cut(s) 2715
Bsu36I CCTNAGG 2 cut(s) 1592, 3088
BsuRI GGCC 6 cut(s) 62, 107, 140, 325, 1008, 1025
BsuTUI ATCGAT 1 cut(s) 2715
BtgI CCRYGG 2 cut(s) 2949, 3223
BtsCI GGATG 7 cut(s) 260, 365, 556, 1437, 1701, 2959, 3237
BtsI GCAGTG 2 cut(s) 503, 2650
BtsIMutI CAGTG 4 cut(s) 503, 1869, 2044, 2650
BveI ACCTGC 2 cut(s) 713, 2752
Cac8I GCNNGC 8 cut(s) 60, 169, 224, 1006, 1010, 1027, 1393, 2181
CciI TCATGA 3 cut(s) 1618, 2059, 2278
CfoI GCGC 7 cut(s) 173, 224, 226, 1266, 2230, 2728, 2959
Cfr10I RCCGGY 4 cut(s) 62, 167, 1008, 1021
Cfr13I GGNCC 3 cut(s) 40, 301, 589
ClaI ATCGAT 1 cut(s) 2715
Csp6I GTAC 5 cut(s) 1077, 1464, 1632, 2319, 2486
CviQI GTAC 5 cut(s) 1077, 1464, 1632, 2319, 2486
DpnI GATC 9 cut(s) 21, 74, 422, 965, 1305, 1700, 1777, 2881, 3221
DpnII GATC 9 cut(s) 19, 72, 420, 963, 1303, 1698, 1775, 2879, 3219
DraI TTTAAA 3 cut(s) 1380, 1605, 2662
DraIII CACNNNGTG 1 cut(s) 49
DriI GACNNNNNGTC 1 cut(s) 1159
EaeI YGGCCR 4 cut(s) 105, 323, 1006, 1023
EagI CGGCCG 2 cut(s) 105, 323
Eam1104I CTCTTC 5 cut(s) 980, 1122, 2085, 2134, 3177
Eam1105I GACNNNNNGTC 1 cut(s) 1159
EarI CTCTTC 5 cut(s) 980, 1122, 2085, 2134, 3177
EciI GGCGGA 1 cut(s) 367
Ecl136II GAGCTC 1 cut(s) 151
EclXI CGGCCG 2 cut(s) 105, 323
Eco130I CCWWGG 3 cut(s) 411, 658, 1386
Eco147I AGGCCT 1 cut(s) 140
Eco24I GRGCYC 2 cut(s) 153, 2616
Eco31I GGTCTC 2 cut(s) 207, 1581
Eco32I GATATC 1 cut(s) 2587
Eco47I GGWCC 3 cut(s) 40, 301, 589
Eco47III AGCGCT 1 cut(s) 1265
Eco52I CGGCCG 2 cut(s) 105, 323
Eco53kI GAGCTC 1 cut(s) 151
Eco57I CTGAAG 3 cut(s) 113, 1977, 2328
Eco81I CCTNAGG 2 cut(s) 1592, 3088
Eco88I CYCGRG 3 cut(s) 134, 152, 395
Eco91I GGTNACC 1 cut(s) 700
EcoICRI GAGCTC 1 cut(s) 151
EcoO109I RGGNCCY 1 cut(s) 301
EcoO65I GGTNACC 1 cut(s) 700
EcoRI GAATTC 1 cut(s) 2333
EcoRII CCWGG 6 cut(s) 27, 296, 1149, 1771, 2616, 3145
EcoRV GATATC 1 cut(s) 2587
EcoT14I CCWWGG 3 cut(s) 411, 658, 1386
EcoT38I GRGCYC 2 cut(s) 153, 2616
ErhI CCWWGG 3 cut(s) 411, 658, 1386
Esp3I CGTCTC 2 cut(s) 205, 294
FalI AAGNNNNNCTT 2 cut(s) 2490, 2522
FaqI GGGAC 3 cut(s) 314, 1639, 3058
FauI CCCGC 2 cut(s) 301, 1275
FauNDI CATATG 1 cut(s) 1423
FblI GTMKAC 3 cut(s) 180, 1813, 2440
Fnu4HI GCNGC 9 cut(s) 56, 82, 131, 233, 284, 311, 381, 452, 1403
FokI GGATG 7 cut(s) 247, 372, 563, 1424, 1708, 2966, 3244
FriOI GRGCYC 2 cut(s) 153, 2616
Fsp4HI GCNGC 9 cut(s) 56, 82, 131, 233, 284, 311, 381, 452, 1403
FspBI CTAG 8 cut(s) 86, 465, 653, 1239, 1350, 2156, 2213, 2667
GlaI GCGC 7 cut(s) 172, 223, 225, 1265, 2229, 2727, 2958
GluI GCNGC 9 cut(s) 56, 82, 131, 233, 284, 311, 381, 452, 1403
GsaI CCCAGC 1 cut(s) 1409
GsuI CTGGAG 5 cut(s) 50, 1417, 2126, 2600, 3129
HaeII RGCGCY 2 cut(s) 174, 1267
HaeIII GGCC 6 cut(s) 62, 107, 140, 325, 1008, 1025
HapII CCGG 9 cut(s) 15, 63, 104, 108, 168, 598, 1009, 1022, 3169
HhaI GCGC 7 cut(s) 173, 224, 226, 1266, 2230, 2728, 2959
Hin6I GCGC 7 cut(s) 171, 222, 224, 1264, 2228, 2726, 2957
HinP1I GCGC 7 cut(s) 171, 222, 224, 1264, 2228, 2726, 2957
HincII GTYRAC 3 cut(s) 124, 181, 2011
HindII GTYRAC 3 cut(s) 124, 181, 2011
HindIII AAGCTT 1 cut(s) 2504
HpaII CCGG 9 cut(s) 15, 63, 104, 108, 168, 598, 1009, 1022, 3169
HphI GGTGA 7 cut(s) 4, 483, 694, 1421, 1666, 1770, 2873
Hpy99I CGWCG 2 cut(s) 80, 182
HpyAV CCTTC 8 cut(s) 578, 776, 866, 1360, 1501, 1616, 2852, 3208
HpyCH4III ACNGT 4 cut(s) 1487, 1636, 1842, 3238
HpyCH4IV ACGT 8 cut(s) 120, 198, 1670, 2002, 2017, 2655, 2904, 2989
HpyF10VI GCNNNNNNNGC 5 cut(s) 232, 1572, 2156, 2189, 3090
HpySE526I ACGT 8 cut(s) 120, 198, 1670, 2002, 2017, 2655, 2904, 2989
HspAI GCGC 7 cut(s) 171, 222, 224, 1264, 2228, 2726, 2957
KflI GGGWCCC 1 cut(s) 301
KroI GCCGGC 2 cut(s) 167, 1008
KroNI GCCGGC 2 cut(s) 169, 1010
Kzo9I GATC 9 cut(s) 19, 72, 420, 963, 1303, 1698, 1775, 2879, 3219
LmnI GCTCC 8 cut(s) 52, 178, 349, 2619, 2758, 3148, 3155, 3171
Lsp1109I GCAGC 5 cut(s) 67, 93, 244, 270, 1414
LweI GCATC 7 cut(s) 350, 433, 1312, 1454, 1877, 2044, 2944
MaeI CTAG 8 cut(s) 86, 465, 653, 1239, 1350, 2156, 2213, 2667
MaeII ACGT 8 cut(s) 120, 198, 1670, 2002, 2017, 2655, 2904, 2989
MaeIII GTNAC 8 cut(s) 10, 700, 774, 925, 1166, 2143, 3046, 3238
MalI GATC 9 cut(s) 21, 74, 422, 965, 1305, 1700, 1777, 2881, 3221
MboI GATC 9 cut(s) 19, 72, 420, 963, 1303, 1698, 1775, 2879, 3219
MfeI CAATTG 2 cut(s) 612, 1116
MhlI GDGCHC 6 cut(s) 153, 895, 1666, 2194, 2616, 2904
MlyI GAGTC 7 cut(s) 482, 563, 1148, 1757, 1862, 3110, 3196
MmeI TCCRAC 3 cut(s) 228, 849, 2005
MreI CGCCGGCG 1 cut(s) 167
MroNI GCCGGC 2 cut(s) 167, 1008
MroXI GAANNNNTTC 2 cut(s) 682, 2372
MslI CAYNNNNRTG 1 cut(s) 496
MspA1I CMGCKG 4 cut(s) 235, 313, 1405, 1890
MspCI CTTAAG 1 cut(s) 2746
MspI CCGG 9 cut(s) 15, 63, 104, 108, 168, 598, 1009, 1022, 3169
MspR9I CCNGG 8 cut(s) 29, 104, 109, 298, 1151, 1773, 2618, 3147
MunI CAATTG 2 cut(s) 612, 1116
Mva1269I GAATGC 3 cut(s) 914, 1555, 2208
MvaI CCWGG 6 cut(s) 29, 298, 1151, 1773, 2618, 3147
MvnI CGCG 3 cut(s) 130, 222, 224
MwoI GCNNNNNNNGC 5 cut(s) 232, 1572, 2156, 2189, 3090
NaeI GCCGGC 2 cut(s) 169, 1010
NciI CCSGG 2 cut(s) 104, 109
NdeI CATATG 1 cut(s) 1423
NdeII GATC 9 cut(s) 19, 72, 420, 963, 1303, 1698, 1775, 2879, 3219
NgoMIV GCCGGC 2 cut(s) 167, 1008
NlaIV GGNNCC 6 cut(s) 113, 302, 303, 1929, 2615, 3151
NmuCI GTSAC 5 cut(s) 10, 700, 774, 925, 1166
NspI RCATGY 4 cut(s) 458, 1143, 1517, 1717
OliI CACNNNNGTG 1 cut(s) 496
PaeR7I CTCGAG 3 cut(s) 134, 152, 395
PagI TCATGA 3 cut(s) 1618, 2059, 2278
PaqCI CACCTGC 1 cut(s) 713
PauI GCGCGC 1 cut(s) 222
PceI AGGCCT 1 cut(s) 140
PciI ACATGT 1 cut(s) 1139
PcsI WCGNNNNNNNCGW 3 cut(s) 284, 314, 323
PctI GAATGC 3 cut(s) 914, 1555, 2208
PdiI GCCGGC 2 cut(s) 169, 1010
PdmI GAANNNNTTC 2 cut(s) 682, 2372
PflMI CCANNNNNTGG 2 cut(s) 49, 3159
PkrI GCNGC 9 cut(s) 57, 83, 132, 234, 285, 312, 382, 453, 1404
PleI GAGTC 7 cut(s) 482, 562, 1148, 1756, 1861, 3110, 3195
PpsI GAGTC 7 cut(s) 482, 562, 1148, 1756, 1861, 3110, 3195
Ppu21I YACGTR 2 cut(s) 2656, 2905
PpuMI RGGWCCY 1 cut(s) 301
PscI ACATGT 1 cut(s) 1139
PshBI ATTAAT 2 cut(s) 2073, 2085
PsiI TTATAA 1 cut(s) 2853
Psp124BI GAGCTC 1 cut(s) 153
Psp5II RGGWCCY 1 cut(s) 301
Psp6I CCWGG 6 cut(s) 27, 296, 1149, 1771, 2616, 3145
PspEI GGTNACC 1 cut(s) 700
PspFI CCCAGC 1 cut(s) 1405
PspGI CCWGG 6 cut(s) 27, 296, 1149, 1771, 2616, 3145
PspN4I GGNNCC 6 cut(s) 113, 302, 303, 1929, 2615, 3151
PspPI GGNCC 3 cut(s) 40, 301, 589
PspPPI RGGWCCY 1 cut(s) 301
PspXI VCTCGAGB 2 cut(s) 134, 395
PsrI GAACNNNNNNTAC 2 cut(s) 1624, 1656
PteI GCGCGC 1 cut(s) 222
PvuII CAGCTG 3 cut(s) 235, 1405, 1890
RsaI GTAC 5 cut(s) 1078, 1465, 1633, 2320, 2487
RsaNI GTAC 5 cut(s) 1077, 1464, 1632, 2319, 2486
RseI CAYNNNNRTG 1 cut(s) 496
SacI GAGCTC 1 cut(s) 153
SalI GTCGAC 1 cut(s) 179
SatI GCNGC 9 cut(s) 56, 82, 131, 233, 284, 311, 381, 452, 1403
Sau3AI GATC 9 cut(s) 19, 72, 420, 963, 1303, 1698, 1775, 2879, 3219
Sau96I GGNCC 3 cut(s) 40, 301, 589
ScaI AGTACT 2 cut(s) 1078, 1633
SchI GAGTC 7 cut(s) 482, 563, 1148, 1757, 1862, 3110, 3196
ScrFI CCNGG 8 cut(s) 29, 104, 109, 298, 1151, 1773, 2618, 3147
SduI GDGCHC 6 cut(s) 153, 895, 1666, 2194, 2616, 2904
SfaNI GCATC 7 cut(s) 350, 433, 1312, 1454, 1877, 2044, 2944
SfcI CTRYAG 1 cut(s) 1891
Sfr274I CTCGAG 3 cut(s) 134, 152, 395
SgrAI CRCCGGYG 1 cut(s) 167
SinI GGWCC 3 cut(s) 40, 301, 589
SlaI CTCGAG 3 cut(s) 134, 152, 395
SmiMI CAYNNNNRTG 1 cut(s) 496
SmlI CTYRAG 9 cut(s) 134, 152, 227, 266, 395, 1680, 2444, 2746, 3118
SmoI CTYRAG 9 cut(s) 134, 152, 227, 266, 395, 1680, 2444, 2746, 3118
SseBI AGGCCT 1 cut(s) 140
SspI AATATT 1 cut(s) 805
SspMI CTAG 8 cut(s) 86, 465, 653, 1239, 1350, 2156, 2213, 2667
SstI GAGCTC 1 cut(s) 153
StuI AGGCCT 1 cut(s) 140
StyD4I CCNGG 8 cut(s) 27, 102, 107, 296, 1149, 1771, 2616, 3145
StyI CCWWGG 3 cut(s) 411, 658, 1386
TaaI ACNGT 4 cut(s) 1487, 1636, 1842, 3238
TaiI ACGT 8 cut(s) 123, 201, 1673, 2005, 2020, 2658, 2907, 2992
TatI WGTACW 4 cut(s) 1076, 1463, 1631, 2485
TauI GCSGC 4 cut(s) 133, 313, 383, 454
TscAI CASTG 4 cut(s) 503, 1869, 2044, 2650
TseFI GTSAC 5 cut(s) 10, 700, 774, 925, 1166
TseI GCWGC 5 cut(s) 55, 81, 232, 283, 1402
Tsp45I GTSAC 5 cut(s) 10, 700, 774, 925, 1166
TspGWI ACGGA 6 cut(s) 179, 870, 1649, 2938, 3180, 3240
TspRI CASTG 4 cut(s) 503, 1869, 2044, 2650
Van91I CCANNNNNTGG 2 cut(s) 49, 3159
Vha464I CTTAAG 1 cut(s) 2746
VpaK11BI GGWCC 3 cut(s) 40, 301, 589
VspI ATTAAT 2 cut(s) 2073, 2085
XapI RAATTY 7 cut(s) 766, 814, 1600, 1705, 1833, 2333, 2357
XbaI TCTAGA 1 cut(s) 2212
XceI RCATGY 4 cut(s) 458, 1143, 1517, 1717
XhoI CTCGAG 3 cut(s) 134, 152, 395
XmiI GTMKAC 3 cut(s) 180, 1813, 2440
XmnI GAANNNNTTC 2 cut(s) 682, 2372
XspI CTAG 8 cut(s) 86, 465, 653, 1239, 1350, 2156, 2213, 2667
ZrmI AGTACT 2 cut(s) 1078, 1633
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.