Rw7G001090

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0001818
Physical Location & Seq
Reverse (-)
914540 .. 920267
5728 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G001090.1

Sequence Viewer

Length: 2901 bp
ATGGCGATTGTGACCGGAGATCGGTACCTGGAGAAGCTAGTCCACTTCGTGGAGCAGCAAGCCGGTTCTCTGATCGACGGTTCGTTAGTGCTGAAGCTCAACCCGGCCGGCTTCCACTACGTCCACTCCCGCCTCGAGGCCTTGCACGAGCTCGAGAGCCTCCTCGCCGGCGCTCCCGTCGACTATCTCCGAGCCTATGTCTCCGACCTCGGCGACCACCGCGCCCTCGAGCAGCTCAGGAGGATTCTCAGGCTCCTCACCTCGCTTAAGGTCGTTTCGGTGCTGCCGGCGCCGGCCAGAGACCCCACGCCGCTGTCGTTTTGGCTGTTCGGGAGGCTTAGGGTTTTGGAGCTTCGGGGATGCGATTTGTCCACGTCGGCCGCGAAAGGTCTGCTCGAGTTGAGGAACACCTTGGAGAAGATCATCTGTCACAATTCAACTGATGCTCTGCGCCATGTGTTTGCGAGTAGAATTGCAGAGATAAAGGACTCGCCACTATGGCACCGGTTGTCATTTGTTTCGTGTGCTTGTAATGGCTTGGTTCTGATGGATGAGTCTTTGCAACTACTTCCGGCAGTTGAAACTCTTGACCTCAGCCGAAACAAGTTTGCAAAGATTGACAATCTTCGCAAGTGTGGCAAATTGAAACACCTAGACCTTGGTTTCAATCATCTGAGAACGATTTCGTCATTTGGTGAGGTCACCTCTCGACTACTTAAACTTGTTTTGAGGAACAATGCTCTAACTACATTGCGTGGGATTGAGGCTTTGAAGTCCCTTGAAGGGCTTGATGTTTCCTACAATATTATTTCCAATTTTTCAGAGTTAGAGTTCCTTGGTGGTCTTCCATCTCTACAAAGCTTGTGGCTGGAAGGGAATCCATTATGTTGTGCCAGTTGGTATCGACCACAAGTATTCAGCTATTTCTATAATCCAGAAAAATTGAAGTTAGATGATAAGGAAATCAGCACCAGAGAGTTTTGGAAGAGGCAACTAATTATTGCAAGTAGACAGAAGCGACCTGCCAGCTTTGGGTTTTATTCTCCCATAAAGTGTGATGACAAAGGAGATGCAAGTATCAACAGAAGAAGGAAAAAGGTGTCTCGTCTTGCTTCCATTGTAAGTGAAGAAGGGAGCACATCTCTCTGTTCTGATCAGGAGTCTGTGTCTTGTGACAATGAGATTCAAAGCAGAGAAGAACTTGTTATATCAGATGAGGAAGCTGAAATTGTTGATTTGATGACTAGAGTTGAGCAAATGAAGAAAGAGCGCTCTGTTCTTTGGTTGCGAGAGTTCAAAGAATGGTTGGATCATGCTCCTGAGGATTCTGTGGACAGCAATAGACATAGCGGGACGACATTGCATATTGGCAGGGAAAATTATATAAAAGAAAAGTCAAGCTGGAGGCAGCTTGGTGAAAACTCCAGATTTGTTTCAGACTATGCTCAAGCATCAGGAGATGAGAGTAGTACAAATGTTTTAGATTCAGATAGTTCATTTATGGATATGTCTACTGGTTTGCATGCCCATCACTTTGATCAAACAGGTTCCTTGGGAAATGCTGGTTTTGTCATCCCAGTTGGCATGGATAGAAGAGATCTGAAGGAAACCCTAAAAGATAGTTCACGTGAAGGAACAAGTAGTGTCCCTTTACGAGCAAAAAATTTCCATGGTCACGTATTCACCGGTCAAAGAGGTCATAGGATGGTCGAAAATTTGAGTATGTCATTGACTTCTATAGATGATATTTCTGAATCTCGCTCCTCGTCTGTGTGCCCGGGATCACCACCTCATTATCAAAAGGACATTCTCAACCGTCGTCATAACTTGGAGGAAGAAATTTTACAGTTATCTGCAGAGTCATTTTCAGTGGCATCATCTGATAGTAATACCAGCTGTAGTGAAGACGATAACTATGAGTCTAGGCAGTCCATTCCTGAAGGTCATCAGCTACTGAATAAAAATATGGAAGAACATCCGTCTTCAGATCCTTTTAGGTTGTATGACATGAGATATGAGGTTCCACATGTAAGAGGAAATGACAGGTGTTCAGTTGGAATGGGGGCAGAGAAAATTTCAAATTCAGACCTATCGCTGCAATCACATGCCAGTGATGTTCCTGGTCGTGCTCATCATGGTGAGATTGCTTATTTTGTTGATGAAGAAGGTGATTTGTTAGAGAGGAGAAAACGCAGACAGAAAATAAAAAGAAGAGTTGTTACACTACTAGAAGATGACAAAATGGTTAGGCAAGCTGAAACATTACCAACATTAAATGGTAATATAGAGAATCATGTAACAATACTGGAAGATGAGCAAGATAGTCGATCTTTCTATGGGATTAACCTTGAAGTTATTGGTAAGAACCAGATGTTGACAAATACAAGTAATGTCCCACTAATAAATGATACTACTGGATCTTCTGGGGCTGAATGTTTCTCTTCAATGAGTGATGAATTTATTGAGGACTATTTCAGAAAGAATGTTGCAGATATGGGAAGTAATGAAATTTGCAAGCAGTATATGCGCTGCTATTGTATACTTGAGTTGGATTCTCTATACAGAGAAAGAGAAGTAGCTGTCTTGCTTAGCAGTGAAAACAAAGTGTATGTGCTACTAATTGGTATTGGAGGTGATGGATCAGGCACCATCTTGAATTTGCAGGGTTCTCATAGGGTTGAAGACATAAGAGACGTCGTAGTTGGTGTAGGACTTCAGGTTGTGAGGGTGTATGTCGAGGAGAGTGCTACCTACCTCTTTAAAACCAGAAGCATTGAAAGTTCAAGACAGTTACTTTCAACTTTAACAGTTATCGATTCTTACTCTCCAATCGATAAATTTTGTTTGAGGAGGTTCAGGTTGGACTATTTGAGAAACAAATATGTGGAGGTTCAAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_4

Protein Analysis

966

Amino Acids

109.24

Weight (kDa)

5.79

Isoelectric Point (pI)

56.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_4 PF12799 192 - 231 2.9e-06 Leucine Rich repeats (2 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 2697
AbsI CCTCGAGG 1 cut(s) 134
Acc36I ACCTGC 1 cut(s) 1030
Acc65I GGTACC 1 cut(s) 24
AccB1I GGYRCC 4 cut(s) 24, 289, 501, 2645
AccB7I CCANNNNNTGG 1 cut(s) 49
AccI GTMKAC 4 cut(s) 180, 1009, 1511, 2539
AccII CGCG 2 cut(s) 222, 383
AciI CCGC 5 cut(s) 130, 220, 311, 381, 1350
AclWI GGATC 5 cut(s) 1317, 1789, 1982, 2425, 2647
AcoI YGGCCR 3 cut(s) 105, 294, 378
AcsI RAATTY 9 cut(s) 1663, 1714, 1839, 2073, 2080, 2456, 2508, 2656, 2837
AcuI CTGAAG 5 cut(s) 113, 1622, 1959, 1968, 2699
AcvI CACGTG 1 cut(s) 1628
AcyI GRCGYC 2 cut(s) 290, 2694
AdeI CACNNNGTG 1 cut(s) 49
AfaI GTAC 2 cut(s) 26, 1471
AfeI AGCGCT 1 cut(s) 1271
AfiI CCNNNNNNNGG 5 cut(s) 21, 49, 136, 783, 1032
AflII CTTAAG 1 cut(s) 266
AflIII ACRYGT 1 cut(s) 2026
AgeI ACCGGT 2 cut(s) 504, 1685
AjiI CACGTC 1 cut(s) 375
AjnI CCWGG 2 cut(s) 27, 2119
Alw21I GWGCWC 3 cut(s) 153, 1139, 2131
Alw26I GTCTC 4 cut(s) 205, 294, 1107, 2685
AlwI GGATC 5 cut(s) 1317, 1789, 1982, 2425, 2647
AlwNI CAGNNNCTG 1 cut(s) 1954
Ama87I CYCGRG 5 cut(s) 134, 152, 227, 395, 1777
Aor51HI AGCGCT 1 cut(s) 1271
AoxI GGCC 4 cut(s) 105, 138, 294, 378
ApeKI GCWGC 6 cut(s) 55, 232, 283, 1408, 2095, 2529
ApoI RAATTY 9 cut(s) 1663, 1714, 1839, 2073, 2080, 2456, 2508, 2656, 2837
AsiGI ACCGGT 2 cut(s) 504, 1685
Asp700I GAANNNNTTC 1 cut(s) 682
Asp718I GGTACC 1 cut(s) 24
AspLEI GCGC 6 cut(s) 173, 224, 292, 453, 1272, 2529
AsuC2I CCSGG 3 cut(s) 104, 1778, 1779
AsuHPI GGTGA 9 cut(s) 250, 694, 707, 1427, 1675, 1776, 2150, 2180, 2645
AvaI CYCGRG 5 cut(s) 134, 152, 227, 395, 1777
AxyI CCTNAGG 1 cut(s) 1320
BaeGI GKGCMC 1 cut(s) 1778
BanI GGYRCC 4 cut(s) 24, 289, 501, 2645
BanII GRGCYC 1 cut(s) 153
BarI GAAGNNNNNNTAC 4 cut(s) 1827, 1859, 2203, 2235
BauI CACGAG 1 cut(s) 146
BbrPI CACGTG 1 cut(s) 1628
BbsI GAAGAC 4 cut(s) 836, 1911, 1974, 2688
Bbv12I GWGCWC 3 cut(s) 153, 1139, 2131
BbvCI CCTCAGC 1 cut(s) 593
BbvI GCAGC 6 cut(s) 67, 244, 270, 1420, 2082, 2516
BccI CCATC 6 cut(s) 541, 856, 1536, 1699, 2630, 2657
BcgI CGANNNNNNTGC 6 cut(s) 373, 407, 2306, 2340, 2726, 2760
BciT130I CCWGG 2 cut(s) 29, 2121
BclI TGATCA 2 cut(s) 1153, 1537
BcnI CCSGG 3 cut(s) 104, 1778, 1779
BcoDI GTCTC 4 cut(s) 205, 294, 1107, 2685
BfaI CTAG 5 cut(s) 38, 653, 1245, 1923, 2228
BfmI CTRYAG 3 cut(s) 1737, 1854, 1897
BfoI RGCGCY 3 cut(s) 174, 293, 1273
BfrI CTTAAG 1 cut(s) 266
BfuAI ACCTGC 1 cut(s) 1030
BglI GCCNNNNNGGC 1 cut(s) 499
BglII AGATCT 1 cut(s) 1597
BisI GCNGC 8 cut(s) 56, 233, 284, 311, 381, 1409, 2096, 2530
BlpI GCTNAGC 1 cut(s) 2588
BlsI GCNGC 8 cut(s) 57, 234, 285, 312, 382, 1410, 2097, 2531
Bme1390I CCNGG 5 cut(s) 29, 104, 1778, 1779, 2121
BmeT110I CYCGRG 5 cut(s) 134, 152, 227, 395, 1777
BmgBI CACGTC 1 cut(s) 375
BmiI GGNNCC 7 cut(s) 26, 254, 291, 503, 1549, 2022, 2647
BmrFI CCNGG 5 cut(s) 29, 104, 1778, 1779, 2121
BmrI ACTGGG 1 cut(s) 1571
BmsI GCATC 5 cut(s) 350, 433, 1060, 1460, 1883
BmuI ACTGGG 1 cut(s) 1571
BpiI GAAGAC 4 cut(s) 836, 1911, 1974, 2688
BplI GAGNNNNNCTC 4 cut(s) 689, 721, 1126, 1158
BpmI CTGGAG 3 cut(s) 50, 1408, 1423
Bpu10I CCTNAGC 3 cut(s) 236, 338, 593
Bpu1102I GCTNAGC 1 cut(s) 2588
BpuEI CTTGAG 2 cut(s) 1431, 2564
BpuMI CCSGG 3 cut(s) 104, 1778, 1779
Bsa29I ATCGAT 2 cut(s) 2814, 2832
BsaAI YACGTR 2 cut(s) 1628, 1678
BsaHI GRCGYC 2 cut(s) 290, 2694
BsaI GGTCTC 1 cut(s) 294
BsaJI CCNNGG 7 cut(s) 208, 411, 658, 835, 1551, 1669, 1777
BsaWI WCCGGW 3 cut(s) 14, 504, 1685
BsaXI ACNNNNNCTCC 4 cut(s) 110, 140, 1151, 1181
Bsc4I CCNNNNNNNGG 5 cut(s) 21, 49, 136, 783, 1032
Bse118I RCCGGY 7 cut(s) 62, 107, 167, 286, 292, 504, 1685
Bse1I ACTGG 6 cut(s) 894, 1519, 1577, 2109, 2310, 2419
Bse21I CCTNAGG 1 cut(s) 1320
Bse3DI GCAATG 2 cut(s) 749, 1358
BseBI CCWGG 2 cut(s) 29, 2121
BseCI ATCGAT 2 cut(s) 2814, 2832
BseDI CCNNGG 7 cut(s) 208, 411, 658, 835, 1551, 1669, 1777
BseGI GGATG 5 cut(s) 365, 556, 1572, 1710, 1975
BseLI CCNNNNNNNGG 5 cut(s) 21, 49, 136, 783, 1032
BseMI GCAATG 2 cut(s) 749, 1358
BseMII CTCAG 5 cut(s) 250, 262, 607, 665, 1311
BseNI ACTGG 6 cut(s) 894, 1519, 1577, 2109, 2310, 2419
BseRI GAGGAG 6 cut(s) 152, 245, 1753, 2197, 2753, 2863
BseSI GKGCMC 1 cut(s) 1778
BseX3I CGGCCG 2 cut(s) 105, 378
BseXI GCAGC 6 cut(s) 67, 244, 270, 1420, 2082, 2516
Bsh1236I CGCG 2 cut(s) 222, 383
Bsh1285I CGRYCG 2 cut(s) 108, 381
BshFI GGCC 4 cut(s) 107, 140, 296, 380
BshNI GGYRCC 4 cut(s) 24, 289, 501, 2645
BshTI ACCGGT 2 cut(s) 504, 1685
BshVI ATCGAT 2 cut(s) 2814, 2832
BsiEI CGRYCG 2 cut(s) 108, 381
BsiHKAI GWGCWC 3 cut(s) 153, 1139, 2131
BsiHKCI CYCGRG 5 cut(s) 134, 152, 227, 395, 1777
BslFI GGGAC 4 cut(s) 760, 1366, 1631, 2378
BslI CCNNNNNNNGG 5 cut(s) 21, 49, 136, 783, 1032
BsmAI GTCTC 4 cut(s) 205, 294, 1107, 2685
BsmBI CGTCTC 1 cut(s) 2685
BsmFI GGGAC 4 cut(s) 760, 1366, 1631, 2378
BsnI GGCC 4 cut(s) 107, 140, 296, 380
Bso31I GGTCTC 1 cut(s) 294
BsoBI CYCGRG 5 cut(s) 134, 152, 227, 395, 1777
Bsp1286I GDGCHC 4 cut(s) 153, 1139, 1778, 2131
Bsp1720I GCTNAGC 1 cut(s) 2588
Bsp19I CCATGG 1 cut(s) 1669
BspACI CCGC 5 cut(s) 130, 220, 311, 381, 1350
BspANI GGCC 4 cut(s) 107, 140, 296, 380
BspCNI CTCAG 5 cut(s) 249, 261, 606, 666, 1312
BspDI ATCGAT 2 cut(s) 2814, 2832
BspFNI CGCG 2 cut(s) 222, 383
BspLI GGNNCC 7 cut(s) 26, 254, 291, 503, 1549, 2022, 2647
BspMAI CTGCAG 1 cut(s) 1858
BspMI ACCTGC 1 cut(s) 1030
BspPI GGATC 5 cut(s) 1317, 1789, 1982, 2425, 2647
BspT107I GGYRCC 4 cut(s) 24, 289, 501, 2645
BspTI CTTAAG 1 cut(s) 266
BspTNI GGTCTC 1 cut(s) 294
BsrDI GCAATG 2 cut(s) 749, 1358
BsrFI RCCGGY 7 cut(s) 62, 107, 167, 286, 292, 504, 1685
BsrI ACTGG 6 cut(s) 894, 1519, 1577, 2109, 2310, 2419
BssAI RCCGGY 7 cut(s) 62, 107, 167, 286, 292, 504, 1685
BssECI CCNNGG 7 cut(s) 208, 411, 658, 835, 1551, 1669, 1777
BssNAI GTATAC 1 cut(s) 2540
BssNI GRCGYC 2 cut(s) 290, 2694
BssSI CACGAG 1 cut(s) 146
BssT1I CCWWGG 5 cut(s) 411, 658, 835, 1551, 1669
Bst1107I GTATAC 1 cut(s) 2540
Bst2BI CACGAG 1 cut(s) 146
Bst2UI CCWGG 2 cut(s) 29, 2121
Bst4CI ACNGT 5 cut(s) 80, 1817, 1848, 2790, 2809
Bst6I CTCTTC 4 cut(s) 980, 1588, 2206, 2446
BstACI GRCGYC 2 cut(s) 290, 2694
BstAFI CTTAAG 1 cut(s) 266
BstAPI GCANNNNNTGC 1 cut(s) 2524
BstBAI YACGTR 2 cut(s) 1628, 1678
BstC8I GCNNGC 9 cut(s) 60, 109, 169, 288, 294, 1027, 1524, 2253, 2516
BstDEI CTNAG 7 cut(s) 236, 248, 338, 593, 674, 1320, 2588
BstDSI CCRYGG 1 cut(s) 1669
BstEII GGTNACC 1 cut(s) 700
BstF5I GGATG 5 cut(s) 365, 556, 1572, 1710, 1975
BstFNI CGCG 2 cut(s) 222, 383
BstH2I RGCGCY 3 cut(s) 174, 293, 1273
BstHHI GCGC 6 cut(s) 173, 224, 292, 453, 1272, 2529
BstMAI GTCTC 4 cut(s) 205, 294, 1107, 2685
BstMCI CGRYCG 2 cut(s) 108, 381
BstMWI GCNNNNNNNGC 5 cut(s) 219, 289, 499, 636, 2524
BstNI CCWGG 2 cut(s) 29, 2121
BstNSI RCATGY 3 cut(s) 1526, 2030, 2108
BstPI GGTNACC 1 cut(s) 700
BstSCI CCNGG 5 cut(s) 27, 102, 1776, 1777, 2119
BstSFI CTRYAG 3 cut(s) 1737, 1854, 1897
BstSLI GKGCMC 1 cut(s) 1778
BstUI CGCG 2 cut(s) 222, 383
BstV1I GCAGC 6 cut(s) 67, 244, 270, 1420, 2082, 2516
BstV2I GAAGAC 4 cut(s) 836, 1911, 1974, 2688
BstX2I RGATCY 3 cut(s) 1597, 1987, 2417
BstYI RGATCY 3 cut(s) 1597, 1987, 2417
BstZ17I GTATAC 1 cut(s) 2540
BstZI CGGCCG 2 cut(s) 105, 378
Bsu15I ATCGAT 2 cut(s) 2814, 2832
Bsu36I CCTNAGG 1 cut(s) 1320
BsuRI GGCC 4 cut(s) 107, 140, 296, 380
BsuTUI ATCGAT 2 cut(s) 2814, 2832
BtgI CCRYGG 1 cut(s) 1669
BtrI CACGTC 1 cut(s) 375
BtsCI GGATG 5 cut(s) 365, 556, 1572, 1710, 1975
BtsI GCAGTG 1 cut(s) 2599
BtsIMutI CAGTG 3 cut(s) 1875, 2116, 2599
BveI ACCTGC 1 cut(s) 1030
Cac8I GCNNGC 9 cut(s) 60, 109, 169, 288, 294, 1027, 1524, 2253, 2516
CaiI CAGNNNCTG 1 cut(s) 1954
CfoI GCGC 6 cut(s) 173, 224, 292, 453, 1272, 2529
Cfr10I RCCGGY 7 cut(s) 62, 107, 167, 286, 292, 504, 1685
Cfr9I CCCGGG 1 cut(s) 1777
ClaI ATCGAT 2 cut(s) 2814, 2832
Csp6I GTAC 2 cut(s) 25, 1470
CspAI ACCGGT 2 cut(s) 504, 1685
CspCI CAANNNNNGTGG 2 cut(s) 845, 880
CviQI GTAC 2 cut(s) 25, 1470
DdeI CTNAG 7 cut(s) 236, 248, 338, 593, 674, 1320, 2588
DinI GGCGCC 1 cut(s) 291
DraI TTTAAA 1 cut(s) 2761
DraIII CACNNNGTG 1 cut(s) 49
EaeI YGGCCR 3 cut(s) 105, 294, 378
EagI CGGCCG 2 cut(s) 105, 378
Eam1104I CTCTTC 4 cut(s) 980, 1588, 2206, 2446
EarI CTCTTC 4 cut(s) 980, 1588, 2206, 2446
Ecl136II GAGCTC 1 cut(s) 151
EclXI CGGCCG 2 cut(s) 105, 378
Eco130I CCWWGG 5 cut(s) 411, 658, 835, 1551, 1669
Eco147I AGGCCT 1 cut(s) 140
Eco24I GRGCYC 1 cut(s) 153
Eco31I GGTCTC 1 cut(s) 294
Eco47III AGCGCT 1 cut(s) 1271
Eco52I CGGCCG 2 cut(s) 105, 378
Eco53kI GAGCTC 1 cut(s) 151
Eco57I CTGAAG 5 cut(s) 113, 1622, 1959, 1968, 2699
Eco72I CACGTG 1 cut(s) 1628
Eco81I CCTNAGG 1 cut(s) 1320
Eco88I CYCGRG 5 cut(s) 134, 152, 227, 395, 1777
Eco91I GGTNACC 1 cut(s) 700
EcoICRI GAGCTC 1 cut(s) 151
EcoO65I GGTNACC 1 cut(s) 700
EcoRII CCWGG 2 cut(s) 27, 2119
EcoT14I CCWWGG 5 cut(s) 411, 658, 835, 1551, 1669
EcoT38I GRGCYC 1 cut(s) 153
EgeI GGCGCC 1 cut(s) 291
EheI GGCGCC 1 cut(s) 291
ErhI CCWWGG 5 cut(s) 411, 658, 835, 1551, 1669
Esp3I CGTCTC 1 cut(s) 2685
FaqI GGGAC 4 cut(s) 760, 1366, 1631, 2378
FauI CCCGC 2 cut(s) 137, 1343
FbaI TGATCA 2 cut(s) 1153, 1537
FblI GTMKAC 4 cut(s) 180, 1009, 1511, 2539
Fnu4HI GCNGC 8 cut(s) 56, 233, 284, 311, 381, 1409, 2096, 2530
FokI GGATG 5 cut(s) 372, 563, 1559, 1717, 1962
FriOI GRGCYC 1 cut(s) 153
Fsp4HI GCNGC 8 cut(s) 56, 233, 284, 311, 381, 1409, 2096, 2530
FspBI CTAG 5 cut(s) 38, 653, 1245, 1923, 2228
GlaI GCGC 6 cut(s) 172, 223, 291, 452, 1271, 2528
GluI GCNGC 8 cut(s) 56, 233, 284, 311, 381, 1409, 2096, 2530
GsuI CTGGAG 3 cut(s) 50, 1408, 1423
HaeII RGCGCY 3 cut(s) 174, 293, 1273
HaeIII GGCC 4 cut(s) 107, 140, 296, 380
HhaI GCGC 6 cut(s) 173, 224, 292, 453, 1272, 2529
Hin1I GRCGYC 2 cut(s) 290, 2694
Hin6I GCGC 6 cut(s) 171, 222, 290, 451, 1270, 2527
HinP1I GCGC 6 cut(s) 171, 222, 290, 451, 1270, 2527
HincII GTYRAC 2 cut(s) 181, 2376
HindII GTYRAC 2 cut(s) 181, 2376
HindIII AAGCTT 1 cut(s) 859
HphI GGTGA 9 cut(s) 250, 694, 707, 1427, 1675, 1776, 2150, 2180, 2645
Hpy99I CGWCG 5 cut(s) 80, 182, 379, 1821, 2699
HpyAV CCTTC 8 cut(s) 776, 866, 1083, 1124, 1597, 1625, 1934, 2159
HpyCH4III ACNGT 5 cut(s) 80, 1817, 1848, 2790, 2809
HpyCH4IV ACGT 5 cut(s) 120, 374, 1627, 1677, 2694
HpyF10VI GCNNNNNNNGC 5 cut(s) 219, 289, 499, 636, 2524
HpyF3I CTNAG 7 cut(s) 236, 248, 338, 593, 674, 1320, 2588
HpySE526I ACGT 5 cut(s) 120, 374, 1627, 1677, 2694
Hsp92I GRCGYC 2 cut(s) 290, 2694
HspAI GCGC 6 cut(s) 171, 222, 290, 451, 1270, 2527
KasI GGCGCC 1 cut(s) 289
KpnI GGTACC 1 cut(s) 28
KroI GCCGGC 4 cut(s) 107, 167, 286, 292
KroNI GCCGGC 4 cut(s) 109, 169, 288, 294
Ksp22I TGATCA 2 cut(s) 1153, 1537
LmnI GCTCC 7 cut(s) 52, 178, 258, 349, 1134, 1321, 1766
Lsp1109I GCAGC 6 cut(s) 67, 244, 270, 1420, 2082, 2516
LweI GCATC 5 cut(s) 350, 433, 1060, 1460, 1883
MaeI CTAG 5 cut(s) 38, 653, 1245, 1923, 2228
MaeII ACGT 5 cut(s) 120, 374, 1627, 1677, 2694
MaeIII GTNAC 8 cut(s) 10, 428, 700, 1172, 1673, 2218, 2296, 2790
MflI RGATCY 3 cut(s) 1597, 1987, 2417
MhlI GDGCHC 4 cut(s) 153, 1139, 1778, 2131
Mly113I GGCGCC 1 cut(s) 290
MlyI GAGTC 5 cut(s) 482, 563, 1169, 1868, 1928
MmeI TCCRAC 5 cut(s) 228, 1287, 2035, 2529, 2841
MreI CGCCGGCG 1 cut(s) 167
MroNI GCCGGC 4 cut(s) 107, 167, 286, 292
MroXI GAANNNNTTC 1 cut(s) 682
MseI TTAA 6 cut(s) 267, 717, 2273, 2343, 2760, 2804
MslI CAYNNNNRTG 2 cut(s) 2109, 2136
MspA1I CMGCKG 2 cut(s) 313, 1896
MspCI CTTAAG 1 cut(s) 266
MspR9I CCNGG 5 cut(s) 29, 104, 1778, 1779, 2121
MvaI CCWGG 2 cut(s) 29, 2121
MvnI CGCG 2 cut(s) 222, 383
MwoI GCNNNNNNNGC 5 cut(s) 219, 289, 499, 636, 2524
NaeI GCCGGC 4 cut(s) 109, 169, 288, 294
NarI GGCGCC 1 cut(s) 290
NciI CCSGG 3 cut(s) 104, 1778, 1779
NcoI CCATGG 1 cut(s) 1669
NgoMIV GCCGGC 4 cut(s) 107, 167, 286, 292
NlaIV GGNNCC 7 cut(s) 26, 254, 291, 503, 1549, 2022, 2647
NmeAIII GCCGAG 1 cut(s) 189
NmuCI GTSAC 5 cut(s) 10, 428, 700, 1172, 1673
NspI RCATGY 3 cut(s) 1526, 2030, 2108
PaeI GCATGC 1 cut(s) 1526
PaeR7I CTCGAG 4 cut(s) 134, 152, 227, 395
PceI AGGCCT 1 cut(s) 140
PciI ACATGT 1 cut(s) 2026
PcsI WCGNNNNNNNCGW 2 cut(s) 314, 380
PdiI GCCGGC 4 cut(s) 109, 169, 288, 294
PdmI GAANNNNTTC 1 cut(s) 682
PfeI GAWTC 9 cut(s) 244, 877, 1183, 1325, 1484, 1754, 2290, 2552, 2816
PflMI CCANNNNNTGG 1 cut(s) 49
PinAI ACCGGT 2 cut(s) 504, 1685
PkrI GCNGC 8 cut(s) 57, 234, 285, 312, 382, 1410, 2097, 2531
PleI GAGTC 5 cut(s) 482, 562, 1168, 1867, 1927
PluTI GGCGCC 1 cut(s) 293
PmaCI CACGTG 1 cut(s) 1628
PmlI CACGTG 1 cut(s) 1628
PpsI GAGTC 5 cut(s) 482, 562, 1168, 1867, 1927
Ppu21I YACGTR 2 cut(s) 1628, 1678
PscI ACATGT 1 cut(s) 2026
Psp124BI GAGCTC 1 cut(s) 153
Psp6I CCWGG 2 cut(s) 27, 2119
PspCI CACGTG 1 cut(s) 1628
PspEI GGTNACC 1 cut(s) 700
PspGI CCWGG 2 cut(s) 27, 2119
PspN4I GGNNCC 7 cut(s) 26, 254, 291, 503, 1549, 2022, 2647
PspXI VCTCGAGB 3 cut(s) 134, 227, 395
PstI CTGCAG 1 cut(s) 1858
PstNI CAGNNNCTG 1 cut(s) 1954
PsuI RGATCY 3 cut(s) 1597, 1987, 2417
PvuII CAGCTG 1 cut(s) 1896
RsaI GTAC 2 cut(s) 26, 1471
RsaNI GTAC 2 cut(s) 25, 1470
RseI CAYNNNNRTG 2 cut(s) 2109, 2136
SacI GAGCTC 1 cut(s) 153
SalI GTCGAC 1 cut(s) 179
SaqAI TTAA 6 cut(s) 267, 717, 2273, 2343, 2760, 2804
SatI GCNGC 8 cut(s) 56, 233, 284, 311, 381, 1409, 2096, 2530
SchI GAGTC 5 cut(s) 482, 563, 1169, 1868, 1928
ScrFI CCNGG 5 cut(s) 29, 104, 1778, 1779, 2121
SduI GDGCHC 4 cut(s) 153, 1139, 1778, 2131
SfaNI GCATC 5 cut(s) 350, 433, 1060, 1460, 1883
SfcI CTRYAG 3 cut(s) 1737, 1854, 1897
SfoI GGCGCC 1 cut(s) 291
Sfr274I CTCGAG 4 cut(s) 134, 152, 227, 395
SgrAI CRCCGGYG 1 cut(s) 167
SlaI CTCGAG 4 cut(s) 134, 152, 227, 395
SmaI CCCGGG 1 cut(s) 1779
SmiMI CAYNNNNRTG 2 cut(s) 2109, 2136
SmlI CTYRAG 7 cut(s) 134, 152, 227, 266, 395, 1446, 2543
SmoI CTYRAG 7 cut(s) 134, 152, 227, 266, 395, 1446, 2543
SphI GCATGC 1 cut(s) 1526
SseBI AGGCCT 1 cut(s) 140
SsiI CCGC 5 cut(s) 130, 220, 311, 381, 1350
SspDI GGCGCC 1 cut(s) 289
SspI AATATT 1 cut(s) 805
SspMI CTAG 5 cut(s) 38, 653, 1245, 1923, 2228
SstI GAGCTC 1 cut(s) 153
StuI AGGCCT 1 cut(s) 140
StyD4I CCNGG 5 cut(s) 27, 102, 1776, 1777, 2119
StyI CCWWGG 5 cut(s) 411, 658, 835, 1551, 1669
TaaI ACNGT 5 cut(s) 80, 1817, 1848, 2790, 2809
TaiI ACGT 5 cut(s) 123, 377, 1630, 1680, 2697
TatI WGTACW 1 cut(s) 1469
TauI GCSGC 2 cut(s) 313, 383
TfiI GAWTC 9 cut(s) 244, 877, 1183, 1325, 1484, 1754, 2290, 2552, 2816
Tru1I TTAA 6 cut(s) 267, 717, 2273, 2343, 2760, 2804
Tru9I TTAA 6 cut(s) 267, 717, 2273, 2343, 2760, 2804
TscAI CASTG 3 cut(s) 1875, 2116, 2599
TseFI GTSAC 5 cut(s) 10, 428, 700, 1172, 1673
TseI GCWGC 6 cut(s) 55, 232, 283, 1408, 2095, 2529
Tsp45I GTSAC 5 cut(s) 10, 428, 700, 1172, 1673
TspDTI ATGAA 5 cut(s) 1274, 1485, 2175, 2469, 2520
TspGWI ACGGA 1 cut(s) 1968
TspMI CCCGGG 1 cut(s) 1777
TspRI CASTG 3 cut(s) 1875, 2116, 2599
Van91I CCANNNNNTGG 1 cut(s) 49
Vha464I CTTAAG 1 cut(s) 266
XapI RAATTY 9 cut(s) 1663, 1714, 1839, 2073, 2080, 2456, 2508, 2656, 2837
XceI RCATGY 3 cut(s) 1526, 2030, 2108
XhoI CTCGAG 4 cut(s) 134, 152, 227, 395
XmaI CCCGGG 1 cut(s) 1777
XmiI GTMKAC 4 cut(s) 180, 1009, 1511, 2539
XmnI GAANNNNTTC 1 cut(s) 682
XspI CTAG 5 cut(s) 38, 653, 1245, 1923, 2228
ZraI GACGTC 1 cut(s) 2695
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.