MD06G1228300.v1.1

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0001818
Physical Location & Seq
Forward (+)
35838008 .. 35843562
5555 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1228300.v1.1.491

Sequence Viewer

Length: 3261 bp
ATGGCGGTTGTCACCGGAGATCGCTACCTGGAGAAGCTAGTCCACTTCGTGGAGCAGCAGGCCGGTTCGTTGATCGACGGGAGCCTAGTGCTGAAGCTGAACCCGGCCGGGTTCCACTACGTCAACTCCCGGCTCGAGGCCTTGCACGAACTCGAGAGCCTCCTCGCCGGCGCTCCCGTTGACTACCTCCGCGCCTACGTCTCCGACCTTGGAGACCACCGCGCGCTCGAGCAGCTGAGGAGGATTCTCAGGCTCTTGACATCCCTCAAGGTCGTTTCGGTGCTGCCGCCTGCTGCCAGGGACCCTACGCCGCTGTCGTTTTGGCCGTTTGGGAGGCTTAAGGTTTTGGAGCTTCGAGGATGCGATTTGTCCACCTCCGCCGCGAAAGGGTTACTGGAGCTGCGTCACACCTTGGAGAAGATCATTTGCCATAATTCTACTGATGCTTTGCGGCATGTGTTTGCGAGTAGAATTGCAGAGATTAAGGACTCGCCGCAGTGGAACCGCCTGTCATTCATTTCGTGTGCTTGTAATGGCTTGGTTCTCATGGATGAGTCTTTGCAACTTCTTCCTGCTGTCGAAACTCTCGACCTTAGCCGGAACAAGTTTGCAAAGGTTGATAATCTTCGCAAGTGTGTCAAATTGAAGCACCTAGACCTCGGTTTCAATCAGCTCAGAACGATTTCGTTATTCGGTCAGGTCACGTGCCGTTTACTTAAACTTGTTTTGAGGAACAATGCTATAACTACATTGCGTGGGATTGAGAATTTGAAGTCACTGGAAGGGCTTGACGTTTCCTACAATATTCTCTTCAATTTTTCCGAGTTAGAATACCTTTCTGGACTTCGATCTCTTGAAAACTTGTGGTTGGAAGGGAATCCATTATGTTGTGCCAGTTGGTATCGGTCACAAGCATTCAGCTATGTCACTAATCCAGAAAAATTGAAGTTAGATGATAAGGAGATCAGCACCAGAGAATTTTGGAAGAGGCAACTTATTATTGCTAGCAGGCACAAGCGACCTGCCAGCTTTGGGTTTTATTCTCCTGCAAAGTGCGATGATAAAGGAGATTCAAGTATTAACAAAAAAAAGAAAAAGGTATCTCGTCTTGCATCAATTGTTAATGAAGAGGAGAACACATGTTCTGACCAGGACTCTGTGTCTTGTGAGAATGAGATCCAAAGCAGAGAGGATGTTGTCATATCTGACGATGAAGCTGAAATTTTTGATTTGATGACTAGAGTTGAGCTAATGAAGAAAGAGCGCTCCGTTCTTTGGTTGCGGGAGTTCAAGGAATGGTTAGATCGTGCTTCTGAGAATGATGCAGACATCAGTAGATATAGCAGGGCTAGATTGCATCTTGAGAAGGAAAAGTATATAAAAAACAAGGCAAGTTGGAGGCAGCTAGGTGAAAAATCAACATACGTTCAAGCATCAGGAGACGAGAGTAATACAAATGTTTTAGAATCAGTGGGGAATGCTGGTAGGGTCAGCCCAGTTGGCATGGATAGTAGAGATATCGGGGAGAACTTAAAAGCATATTCGTATGAAAGCCCAGTTGGCATGGATGGTAGAGATATTGAGGAGAACTTAAAAGCATATTCGTATGAAGGGACAAGTACTTTCAGTTCAGAGGCTAAAAGTTCCCATACTCAGACATTCACCACTGATGGAGGTCATAGGATGGTCCAAAATTTGAGTATGTCTGCATTGTCTGTCATTGATGATATTTCAGAGTCTTATTCATCATCTGCTAACCCAGGATCACCGCCCCATTATCAAAAGGACATTCTTCATCGTCGACATAACTTAGAGGTGGAAATTTTACAGTTATCCGCAGAGTCCTATTCAGTGGCATCATCTGATAGTAATACGAGCTCAAGTGAAGATGAAAATTGTGAATCTAGGCAGTCAGTTCCTGAGAACTTGTTGAATGAGAGTGCCGAAGAATACCCATCTAAAAATTGTTTCAAGTATTATGACATAAAACATGAGATTGACAGACGTTCGGTCAGTGTACGTGCCAGTGACATTCCTGCTGATGCTCATGATGTTGAAATTACTAATTGCATTAATGAAGAGGGTGATTTGCTGGAGAGGAGGAAAGGAAGACAGAGGACTAAAAGAAGAGTTATCGCTATAGTAGAAGATGATAACATGATTAGGCAACCAGAACTGTCACCAAAATCAAATGGCAATCTAGGTAATCATGTAGGGCAAGTCGAAAATAAGCAAGAGAATAAATATTTCTATGGGAGTGATTTTCAAGAAGTTGTTGATAAGAAACAGATGTTGGCAAATAGAAGCAGTGCCTCAATAACCGAAATCTTATCTTCAGGAAGTGATGAATTTATTGAGAATTATTTCAATACAAATATCGCAGATTCACGAAATCACGAGATCTGTAGGCATTATTTGTGTTGCTGTTGTATACTTGAGCGGGATTTTCTTTCCCCAGAAAGAGAAGTTGCTGTTGTACTTAGTAGTGAAGACAAGCTTTATATACTGCCCATTGGTATTGCAGGAGATGGGTCAGGCACCATCTTAAAGTTACAGGGTCGTCACAGGGTTGAGGATATCAGAGAAGTTGTAGTTGGTATAGGGCTCCAGGTTGTGAGGGTGTATGTTGGAGGCAGTGCGAGTTACCTGTTTAAAACTAGAAGCATTGAAAAATCAAGACAGTTACTTTCTACTTTAAAAGTTATCGATTCTTTTGCCCCAAATGGAGAACTTTGCTTAAGGAGTTTGGAGCAGGTTCAGGTGGAGTTATTTGAGAAACAAATATGTGGAGGTTCAAAAGTGAGCATATTCCAGTATTCTATGGTACAGTTTTGGTGTAGTTATAATGAAGGTGAATCATGGTTTTCAAGATCATTATTTGTGGCTGGAGGGCACGTATTTTTGTGCTTTGAAGACCTTATGCAGTTCAGCTCTTTATGCGTGGATGCGTCTTTGCCCCCATATTTTTCACTTGACTTGTGTTGCTCTATTGCCGACATATCTGAGTTGGTTGTCGATGTCAGAGAAAGTCGGTGTGTAACCATAGCTGTTGCGTGCGCAATGTCGGAGTTCTGCCCCCCAGGCTCAGCCGAAGCGGGCAATTCAGACTCAAGTGTAAATGATAAAAAGATTGCTCCAGGCTCCATGACTTGGAAGCTCCAGTGGTTTTCCGAAGAGAGTCCGTTCAAGTTTGTGGCGTTGTTGAAGGCAATCCATGAAGGGATGACCGCTTCCCCTTTGCTTGTAAGATTTATATCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_4

Protein Analysis

1087

Amino Acids

122.19

Weight (kDa)

5.56

Isoelectric Point (pI)

51.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_4 PF12799 192 - 229 9e-07 Leucine Rich repeats (2 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2844
Acc16I TGCGCA 1 cut(s) 3058
Acc36I ACCTGC 2 cut(s) 1030, 2743
AccB1I GGYRCC 1 cut(s) 2537
AccB7I CCANNNNNTGG 2 cut(s) 49, 3150
AccBSI CCGCTC 1 cut(s) 2440
AccI GTMKAC 2 cut(s) 1801, 2431
AccII CGCG 4 cut(s) 192, 222, 224, 383
AclWI GGATC 2 cut(s) 1171, 1771
AcoI YGGCCR 2 cut(s) 105, 323
AcsI RAATTY 6 cut(s) 766, 977, 1221, 1693, 1821, 2348
AcuI CTGAAG 2 cut(s) 113, 2319
AcvI CACGTG 1 cut(s) 705
AdeI CACNNNGTG 1 cut(s) 49
AfaI GTAC 4 cut(s) 1621, 2019, 2478, 2826
AfeI AGCGCT 1 cut(s) 1265
AfiI CCNNNNNNNGG 6 cut(s) 49, 136, 387, 1032, 1275, 3150
AflII CTTAAG 2 cut(s) 338, 2737
AflIII ACRYGT 1 cut(s) 1139
AhdI GACNNNNNGTC 2 cut(s) 1159, 2009
AjnI CCWGG 7 cut(s) 27, 296, 1149, 1759, 2607, 3079, 3136
AjuI GAANNNNNNNTTGG 6 cut(s) 1542, 1574, 2276, 2308, 2577, 2609
Alw21I GWGCWC 1 cut(s) 1880
Alw26I GTCTC 3 cut(s) 205, 207, 1434
AlwI GGATC 2 cut(s) 1171, 1771
AlwNI CAGNNNCTG 1 cut(s) 1919
Ama87I CYCGRG 3 cut(s) 134, 152, 227
Aor51HI AGCGCT 1 cut(s) 1265
AoxI GGCC 4 cut(s) 60, 105, 138, 323
ApeKI GCWGC 6 cut(s) 55, 232, 283, 293, 400, 1402
ApoI RAATTY 6 cut(s) 766, 977, 1221, 1693, 1821, 2348
AseI ATTAAT 1 cut(s) 2073
Asp700I GAANNNNTTC 2 cut(s) 682, 2363
AspLEI GCGC 6 cut(s) 173, 194, 224, 226, 1266, 3059
AspS9I GGNCC 2 cut(s) 301, 1687
AsuC2I CCSGG 3 cut(s) 104, 109, 130
AsuHPI GGTGA 7 cut(s) 4, 1421, 1654, 1758, 2096, 2172, 2864
AsuNHI GCTAGC 1 cut(s) 1004
AvaI CYCGRG 3 cut(s) 134, 152, 227
AvaII GGWCC 2 cut(s) 301, 1687
BaeGI GKGCMC 1 cut(s) 2895
BanI GGYRCC 1 cut(s) 2537
BanII GRGCYC 2 cut(s) 1880, 2607
BauI CACGAG 1 cut(s) 2396
BbrPI CACGTG 1 cut(s) 705
BbsI GAAGAC 3 cut(s) 2116, 2496, 2919
Bbv12I GWGCWC 1 cut(s) 1880
BbvCI CCTCAGC 1 cut(s) 236
BbvI GCAGC 6 cut(s) 67, 244, 270, 280, 387, 1414
BccI CCATC 6 cut(s) 1562, 1664, 1678, 1963, 2522, 2549
BceAI ACGGC 2 cut(s) 310, 693
BcgI CGANNNNNNTGC 2 cut(s) 2696, 2730
BciT130I CCWGG 7 cut(s) 29, 298, 1151, 1761, 2609, 3081, 3138
BcnI CCSGG 3 cut(s) 104, 109, 130
BcoDI GTCTC 3 cut(s) 205, 207, 1434
BfmI CTRYAG 2 cut(s) 2139, 2404
BfoI RGCGCY 2 cut(s) 174, 1267
BfrI CTTAAG 2 cut(s) 338, 2737
BfuAI ACCTGC 2 cut(s) 1030, 2743
BglI GCCNNNNNGGC 3 cut(s) 1500, 1560, 3081
BglII AGATCT 1 cut(s) 2400
BlpI GCTNAGC 1 cut(s) 3085
BmcAI AGTACT 1 cut(s) 1621
Bme18I GGWCC 2 cut(s) 301, 1687
BmeRI GACNNNNNGTC 2 cut(s) 1159, 2009
BmeT110I CYCGRG 3 cut(s) 134, 152, 227
BmgT120I GGNCC 2 cut(s) 301, 1687
BmiI GGNNCC 8 cut(s) 83, 113, 302, 303, 503, 2539, 2606, 3142
BmrI ACTGGG 2 cut(s) 1490, 1550
BmsI GCATC 9 cut(s) 350, 433, 1121, 1312, 1366, 1442, 1865, 2032, 2935
BmtI GCTAGC 1 cut(s) 1008
BmuI ACTGGG 2 cut(s) 1490, 1550
BpiI GAAGAC 3 cut(s) 2116, 2496, 2919
BpmI CTGGAG 7 cut(s) 50, 416, 2114, 2591, 2907, 3120, 3143
Bpu10I CCTNAGC 2 cut(s) 236, 593
Bpu1102I GCTNAGC 1 cut(s) 3085
BpuEI CTTGAG 5 cut(s) 251, 1382, 1864, 2456, 3094
BpuMI CCSGG 3 cut(s) 104, 109, 130
Bsa29I ATCGAT 1 cut(s) 2706
BsaAI YACGTR 3 cut(s) 705, 2021, 2896
BsaBI GATNNNNATC 1 cut(s) 3253
BsaI GGTCTC 1 cut(s) 207
BsaJI CCNNGG 6 cut(s) 208, 297, 411, 658, 1759, 3079
BsaWI WCCGGW 1 cut(s) 14
BsaXI ACNNNNNCTCC 4 cut(s) 110, 140, 2880, 2910
Bsc4I CCNNNNNNNGG 6 cut(s) 49, 136, 387, 1032, 1275, 3150
Bse118I RCCGGY 2 cut(s) 62, 167
Bse1I ACTGG 8 cut(s) 399, 783, 894, 1496, 1556, 2025, 2812, 3160
Bse3DI GCAATG 2 cut(s) 749, 3066
Bse8I GATNNNNATC 1 cut(s) 3253
BseBI CCWGG 7 cut(s) 29, 298, 1151, 1761, 2609, 3081, 3138
BseCI ATCGAT 1 cut(s) 2706
BseDI CCNNGG 6 cut(s) 208, 297, 411, 658, 1759, 3079
BseGI GGATG 8 cut(s) 260, 365, 556, 1198, 1573, 1689, 2950, 3228
BseJI GATNNNNATC 1 cut(s) 3253
BseLI CCNNNNNNNGG 6 cut(s) 49, 136, 387, 1032, 1275, 3150
BseMI GCAATG 2 cut(s) 749, 3066
BseMII CTCAG 8 cut(s) 227, 262, 688, 1305, 1667, 1911, 2994, 3099
BseNI ACTGG 8 cut(s) 399, 783, 894, 1496, 1556, 2025, 2812, 3160
BsePI GCGCGC 1 cut(s) 222
BseRI GAGGAG 5 cut(s) 152, 253, 1145, 1598, 2113
BseSI GKGCMC 1 cut(s) 2895
BseX3I CGGCCG 1 cut(s) 105
BseXI GCAGC 6 cut(s) 67, 244, 270, 280, 387, 1414
Bsh1236I CGCG 4 cut(s) 192, 222, 224, 383
Bsh1285I CGRYCG 1 cut(s) 108
BshFI GGCC 4 cut(s) 62, 107, 140, 325
BshNI GGYRCC 1 cut(s) 2537
BshVI ATCGAT 1 cut(s) 2706
BsiEI CGRYCG 1 cut(s) 108
BsiHKAI GWGCWC 1 cut(s) 1880
BsiHKCI CYCGRG 3 cut(s) 134, 152, 227
BsiSI CCGG 7 cut(s) 15, 63, 104, 108, 130, 168, 598
BslFI GGGAC 2 cut(s) 314, 1627
BslI CCNNNNNNNGG 6 cut(s) 49, 136, 387, 1032, 1275, 3150
BsmAI GTCTC 3 cut(s) 205, 207, 1434
BsmBI CGTCTC 2 cut(s) 205, 1434
BsmFI GGGAC 2 cut(s) 314, 1627
BsmI GAATGC 2 cut(s) 914, 1483
BsnI GGCC 4 cut(s) 62, 107, 140, 325
Bso31I GGTCTC 1 cut(s) 207
BsoBI CYCGRG 3 cut(s) 134, 152, 227
Bsp1286I GDGCHC 3 cut(s) 1880, 2607, 2895
Bsp1720I GCTNAGC 1 cut(s) 3085
BspANI GGCC 4 cut(s) 62, 107, 140, 325
BspCNI CTCAG 8 cut(s) 228, 261, 687, 1306, 1666, 1912, 2995, 3098
BspDI ATCGAT 1 cut(s) 2706
BspFNI CGCG 4 cut(s) 192, 222, 224, 383
BspHI TCATGA 1 cut(s) 2047
BspLI GGNNCC 8 cut(s) 83, 113, 302, 303, 503, 2539, 2606, 3142
BspMI ACCTGC 2 cut(s) 1030, 2743
BspOI GCTAGC 1 cut(s) 1008
BspPI GGATC 2 cut(s) 1171, 1771
BspT107I GGYRCC 1 cut(s) 2537
BspTI CTTAAG 2 cut(s) 338, 2737
BspTNI GGTCTC 1 cut(s) 207
BsrBI CCGCTC 1 cut(s) 2440
BsrDI GCAATG 2 cut(s) 749, 3066
BsrFI RCCGGY 2 cut(s) 62, 167
BsrI ACTGG 8 cut(s) 399, 783, 894, 1496, 1556, 2025, 2812, 3160
BssAI RCCGGY 2 cut(s) 62, 167
BssECI CCNNGG 6 cut(s) 208, 297, 411, 658, 1759, 3079
BssHII GCGCGC 1 cut(s) 222
BssNAI GTATAC 1 cut(s) 2432
BssSI CACGAG 1 cut(s) 2396
BssT1I CCWWGG 2 cut(s) 208, 411
Bst1107I GTATAC 1 cut(s) 2432
Bst2BI CACGAG 1 cut(s) 2396
Bst2UI CCWGG 7 cut(s) 29, 298, 1151, 1761, 2609, 3081, 3138
Bst4CI ACNGT 4 cut(s) 1830, 2178, 2682, 2829
Bst6I CTCTTC 6 cut(s) 815, 980, 1122, 2073, 2122, 3168
BstAFI CTTAAG 2 cut(s) 338, 2737
BstBAI YACGTR 3 cut(s) 705, 2021, 2896
BstC8I GCNNGC 9 cut(s) 60, 169, 224, 291, 1006, 1010, 1027, 3055, 3097
BstF5I GGATG 8 cut(s) 260, 365, 556, 1198, 1573, 1689, 2950, 3228
BstFNI CGCG 4 cut(s) 192, 222, 224, 383
BstH2I RGCGCY 2 cut(s) 174, 1267
BstHHI GCGC 6 cut(s) 173, 194, 224, 226, 1266, 3059
BstMAI GTCTC 3 cut(s) 205, 207, 1434
BstMCI CGRYCG 1 cut(s) 108
BstMWI GCNNNNNNNGC 5 cut(s) 232, 1500, 1560, 2937, 3081
BstNI CCWGG 7 cut(s) 29, 298, 1151, 1761, 2609, 3081, 3138
BstNSI RCATGY 2 cut(s) 458, 1143
BstSFI CTRYAG 2 cut(s) 2139, 2404
BstSLI GKGCMC 1 cut(s) 2895
BstUI CGCG 4 cut(s) 192, 222, 224, 383
BstV1I GCAGC 6 cut(s) 67, 244, 270, 280, 387, 1414
BstV2I GAAGAC 3 cut(s) 2116, 2496, 2919
BstX2I RGATCY 2 cut(s) 1176, 2400
BstYI RGATCY 2 cut(s) 1176, 2400
BstZ17I GTATAC 1 cut(s) 2432
BstZI CGGCCG 1 cut(s) 105
Bsu15I ATCGAT 1 cut(s) 2706
BsuRI GGCC 4 cut(s) 62, 107, 140, 325
BsuTUI ATCGAT 1 cut(s) 2706
BtgZI GCGATG 1 cut(s) 1071
BtsCI GGATG 8 cut(s) 260, 365, 556, 1198, 1573, 1689, 2950, 3228
BtsI GCAGTG 3 cut(s) 503, 2314, 2641
BveI ACCTGC 2 cut(s) 1030, 2743
Cac8I GCNNGC 9 cut(s) 60, 169, 224, 291, 1006, 1010, 1027, 3055, 3097
CaiI CAGNNNCTG 1 cut(s) 1919
CciI TCATGA 1 cut(s) 2047
CfoI GCGC 6 cut(s) 173, 194, 224, 226, 1266, 3059
Cfr10I RCCGGY 2 cut(s) 62, 167
Cfr13I GGNCC 2 cut(s) 301, 1687
ClaI ATCGAT 1 cut(s) 2706
CseI GACGC 2 cut(s) 392, 2937
Csp6I GTAC 4 cut(s) 1620, 2018, 2477, 2825
CviQI GTAC 4 cut(s) 1620, 2018, 2477, 2825
DraI TTTAAA 2 cut(s) 2653, 2697
DraIII CACNNNGTG 1 cut(s) 49
DriI GACNNNNNGTC 2 cut(s) 1159, 2009
EaeI YGGCCR 2 cut(s) 105, 323
EagI CGGCCG 1 cut(s) 105
Eam1104I CTCTTC 6 cut(s) 815, 980, 1122, 2073, 2122, 3168
Eam1105I GACNNNNNGTC 2 cut(s) 1159, 2009
EarI CTCTTC 6 cut(s) 815, 980, 1122, 2073, 2122, 3168
EciI GGCGGA 1 cut(s) 367
Ecl136II GAGCTC 1 cut(s) 1878
EclXI CGGCCG 1 cut(s) 105
Eco130I CCWWGG 2 cut(s) 208, 411
Eco147I AGGCCT 1 cut(s) 140
Eco24I GRGCYC 2 cut(s) 1880, 2607
Eco31I GGTCTC 1 cut(s) 207
Eco32I GATATC 2 cut(s) 1519, 2578
Eco47I GGWCC 2 cut(s) 301, 1687
Eco47III AGCGCT 1 cut(s) 1265
Eco52I CGGCCG 1 cut(s) 105
Eco53kI GAGCTC 1 cut(s) 1878
Eco57I CTGAAG 2 cut(s) 113, 2319
Eco72I CACGTG 1 cut(s) 705
Eco88I CYCGRG 3 cut(s) 134, 152, 227
EcoICRI GAGCTC 1 cut(s) 1878
EcoO109I RGGNCCY 1 cut(s) 301
EcoRII CCWGG 7 cut(s) 27, 296, 1149, 1759, 2607, 3079, 3136
EcoRV GATATC 2 cut(s) 1519, 2578
EcoT14I CCWWGG 2 cut(s) 208, 411
EcoT38I GRGCYC 2 cut(s) 1880, 2607
ErhI CCWWGG 2 cut(s) 208, 411
Esp3I CGTCTC 2 cut(s) 205, 1434
FalI AAGNNNNNCTT 2 cut(s) 2481, 2513
FaqI GGGAC 2 cut(s) 314, 1627
FauI CCCGC 3 cut(s) 1275, 2433, 3088
FblI GTMKAC 2 cut(s) 1801, 2431
FokI GGATG 8 cut(s) 247, 372, 563, 1205, 1580, 1696, 2957, 3235
FriOI GRGCYC 2 cut(s) 1880, 2607
FspI TGCGCA 1 cut(s) 3058
GlaI GCGC 6 cut(s) 172, 193, 223, 225, 1265, 3058
GsuI CTGGAG 7 cut(s) 50, 416, 2114, 2591, 2907, 3120, 3143
HaeII RGCGCY 2 cut(s) 174, 1267
HaeIII GGCC 4 cut(s) 62, 107, 140, 325
HapII CCGG 7 cut(s) 15, 63, 104, 108, 130, 168, 598
HgaI GACGC 2 cut(s) 392, 2937
HhaI GCGC 6 cut(s) 173, 194, 224, 226, 1266, 3059
Hin6I GCGC 6 cut(s) 171, 192, 222, 224, 1264, 3057
HinP1I GCGC 6 cut(s) 171, 192, 222, 224, 1264, 3057
HincII GTYRAC 3 cut(s) 124, 181, 1802
HindII GTYRAC 3 cut(s) 124, 181, 1802
HindIII AAGCTT 1 cut(s) 2495
HpaII CCGG 7 cut(s) 15, 63, 104, 108, 130, 168, 598
HphI GGTGA 7 cut(s) 4, 1421, 1654, 1758, 2096, 2172, 2864
Hpy166II GTNNAC 8 cut(s) 43, 124, 181, 372, 713, 1802, 2018, 2432
Hpy8I GTNNAC 8 cut(s) 43, 124, 181, 372, 713, 1802, 2018, 2432
Hpy99I CGWCG 2 cut(s) 80, 1803
HpyAV CCTTC 7 cut(s) 776, 866, 1360, 1604, 2843, 3199, 3212
HpyCH4III ACNGT 4 cut(s) 1830, 2178, 2682, 2829
HpyCH4IV ACGT 8 cut(s) 120, 198, 704, 792, 1425, 2005, 2020, 2895
HpyF10VI GCNNNNNNNGC 5 cut(s) 232, 1500, 1560, 2937, 3081
HpySE526I ACGT 8 cut(s) 120, 198, 704, 792, 1425, 2005, 2020, 2895
HspAI GCGC 6 cut(s) 171, 192, 222, 224, 1264, 3057
KflI GGGWCCC 1 cut(s) 301
KroI GCCGGC 1 cut(s) 167
KroNI GCCGGC 1 cut(s) 169
Lsp1109I GCAGC 6 cut(s) 67, 244, 270, 280, 387, 1414
LweI GCATC 9 cut(s) 350, 433, 1121, 1312, 1366, 1442, 1865, 2032, 2935
MaeII ACGT 8 cut(s) 120, 198, 704, 792, 1425, 2005, 2020, 2895
MbiI CCGCTC 1 cut(s) 2440
MfeI CAATTG 1 cut(s) 1116
MflI RGATCY 2 cut(s) 1176, 2400
MhlI GDGCHC 3 cut(s) 1880, 2607, 2895
MlyI GAGTC 7 cut(s) 482, 563, 1148, 1745, 1850, 3101, 3187
MmeI TCCRAC 5 cut(s) 228, 849, 1376, 2608, 3045
MreI CGCCGGCG 1 cut(s) 167
MroNI GCCGGC 1 cut(s) 167
MroXI GAANNNNTTC 2 cut(s) 682, 2363
MspA1I CMGCKG 2 cut(s) 235, 313
MspCI CTTAAG 2 cut(s) 338, 2737
MspI CCGG 7 cut(s) 15, 63, 104, 108, 130, 168, 598
MunI CAATTG 1 cut(s) 1116
Mva1269I GAATGC 2 cut(s) 914, 1483
MvaI CCWGG 7 cut(s) 29, 298, 1151, 1761, 2609, 3081, 3138
MvnI CGCG 4 cut(s) 192, 222, 224, 383
MwoI GCNNNNNNNGC 5 cut(s) 232, 1500, 1560, 2937, 3081
NaeI GCCGGC 1 cut(s) 169
NciI CCSGG 3 cut(s) 104, 109, 130
NgoMIV GCCGGC 1 cut(s) 167
NheI GCTAGC 1 cut(s) 1004
NlaIV GGNNCC 8 cut(s) 83, 113, 302, 303, 503, 2539, 2606, 3142
NmuCI GTSAC 9 cut(s) 10, 404, 700, 774, 906, 925, 2027, 2178, 2561
NsbI TGCGCA 1 cut(s) 3058
NspI RCATGY 2 cut(s) 458, 1143
PaeR7I CTCGAG 3 cut(s) 134, 152, 227
PagI TCATGA 1 cut(s) 2047
PauI GCGCGC 1 cut(s) 222
PceI AGGCCT 1 cut(s) 140
PciI ACATGT 1 cut(s) 1139
PcsI WCGNNNNNNNCGW 3 cut(s) 314, 323, 585
PctI GAATGC 2 cut(s) 914, 1483
PdiI GCCGGC 1 cut(s) 169
PdmI GAANNNNTTC 2 cut(s) 682, 2363
PfeI GAWTC 8 cut(s) 244, 877, 1070, 1466, 1901, 2384, 2708, 2855
PflMI CCANNNNNTGG 2 cut(s) 49, 3150
PleI GAGTC 7 cut(s) 482, 562, 1148, 1744, 1849, 3101, 3186
PmaCI CACGTG 1 cut(s) 705
PmlI CACGTG 1 cut(s) 705
PpsI GAGTC 7 cut(s) 482, 562, 1148, 1744, 1849, 3101, 3186
Ppu21I YACGTR 3 cut(s) 705, 2021, 2896
PpuMI RGGWCCY 1 cut(s) 301
PscI ACATGT 1 cut(s) 1139
PshBI ATTAAT 1 cut(s) 2073
PsiI TTATAA 1 cut(s) 2844
Psp124BI GAGCTC 1 cut(s) 1880
Psp5II RGGWCCY 1 cut(s) 301
Psp6I CCWGG 7 cut(s) 27, 296, 1149, 1759, 2607, 3079, 3136
PspCI CACGTG 1 cut(s) 705
PspGI CCWGG 7 cut(s) 27, 296, 1149, 1759, 2607, 3079, 3136
PspN4I GGNNCC 8 cut(s) 83, 113, 302, 303, 503, 2539, 2606, 3142
PspPI GGNCC 2 cut(s) 301, 1687
PspPPI RGGWCCY 1 cut(s) 301
PspXI VCTCGAGB 2 cut(s) 134, 227
PsrI GAACNNNNNNTAC 2 cut(s) 1612, 1644
PstNI CAGNNNCTG 1 cut(s) 1919
PsuI RGATCY 2 cut(s) 1176, 2400
PteI GCGCGC 1 cut(s) 222
PvuII CAGCTG 1 cut(s) 235
RsaI GTAC 4 cut(s) 1621, 2019, 2478, 2826
RsaNI GTAC 4 cut(s) 1620, 2018, 2477, 2825
SacI GAGCTC 1 cut(s) 1880
SalI GTCGAC 1 cut(s) 1800
Sau96I GGNCC 2 cut(s) 301, 1687
ScaI AGTACT 1 cut(s) 1621
SchI GAGTC 7 cut(s) 482, 563, 1148, 1745, 1850, 3101, 3187
SduI GDGCHC 3 cut(s) 1880, 2607, 2895
SfaNI GCATC 9 cut(s) 350, 433, 1121, 1312, 1366, 1442, 1865, 2032, 2935
SfcI CTRYAG 2 cut(s) 2139, 2404
Sfr274I CTCGAG 3 cut(s) 134, 152, 227
SgrAI CRCCGGYG 1 cut(s) 167
SinI GGWCC 2 cut(s) 301, 1687
SlaI CTCGAG 3 cut(s) 134, 152, 227
SseBI AGGCCT 1 cut(s) 140
SspI AATATT 2 cut(s) 805, 2246
SstI GAGCTC 1 cut(s) 1880
StuI AGGCCT 1 cut(s) 140
StyI CCWWGG 2 cut(s) 208, 411
TaaI ACNGT 4 cut(s) 1830, 2178, 2682, 2829
TaiI ACGT 8 cut(s) 123, 201, 707, 795, 1428, 2008, 2023, 2898
TaqII GACCGA 3 cut(s) 683, 894, 1999
TatI WGTACW 2 cut(s) 1619, 2476
TauI GCSGC 5 cut(s) 289, 313, 383, 454, 496
TfiI GAWTC 8 cut(s) 244, 877, 1070, 1466, 1901, 2384, 2708, 2855
TseFI GTSAC 9 cut(s) 10, 404, 700, 774, 906, 925, 2027, 2178, 2561
TseI GCWGC 6 cut(s) 55, 232, 283, 293, 400, 1402
Tsp45I GTSAC 9 cut(s) 10, 404, 700, 774, 906, 925, 2027, 2178, 2561
TspGWI ACGGA 2 cut(s) 1258, 3171
Van91I CCANNNNNTGG 2 cut(s) 49, 3150
Vha464I CTTAAG 2 cut(s) 338, 2737
VpaK11BI GGWCC 2 cut(s) 301, 1687
VspI ATTAAT 1 cut(s) 2073
XapI RAATTY 6 cut(s) 766, 977, 1221, 1693, 1821, 2348
XceI RCATGY 2 cut(s) 458, 1143
XhoI CTCGAG 3 cut(s) 134, 152, 227
XmiI GTMKAC 2 cut(s) 1801, 2431
XmnI GAANNNNTTC 2 cut(s) 682, 2363
ZrmI AGTACT 1 cut(s) 1621
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.