Rh7BG013500

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0001818
Physical Location & Seq
Reverse (-)
862839 .. 869000
6162 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG013500.1

Sequence Viewer

Length: 2859 bp
ATGGCGATTGTGACCGGAGATCGGTACCTGGAGAAGCTAGTCCACTTCGTGGAGCAGCAAGCCGGTTCTCTGATCGACGGTTCGTTAGTGCTGAAGCTCAACCCGGCCGGCTTCCACTACGTCCACTCCCGCCTCGAGGCCTTGCACGAGCTCGAGAGCCTCCTCGCCGGCGCTCCCGTCGACTATCTCCGAGCCTATATCTCCGACCTCGGCGACCACCGCGCCCTCGAGCAGCTCAGGAGGATTCTCAGGCTGTTGACGTCGCTTAAGGTCGTTTCGGTGCTGCCGGCTCCGGCCAGAGACCCCACGCCGCTGTCGTTTTGGCCGTTCGGGAGGCTTAGGGTTTTGGAGCTTCGGGGATGCGATTTGTCCACGTCGGCCGCGAAAGGTCTGCTCGAGTTGAGGCACACCTTGGAGAAGATCATCTGTCACAATTCAACTGATGCTCTGCGGCATGTGTTTGCGAGTAGAATTGCAGAGATAAAGGACTCGCCACTATGGCACCGGTTGTCATTTGTTTCGTGTGCTTGTAATGGCTTGGTTCTGATGGATGAGTCTTTGCAACTACTTCCGGCCGTTGAAACTCTTGACCTGAGCCGAAACAAGTTTGCAAAGATTGACAATCTTCGCAAGTGTGGCAAATTGAAACACCTAGACCTTGGTTTCAATCATCTGAGAACGATTTCGTCATTTGGTGAGGTCACCTCTCGACTACTTAAACTTGTTTTGAGGAACAATGCTCTAACTACGTTGCGTGGGATTGAGACTTTGAAGTCCCTTGAAGGGCTTGATGTTTCCTACAATATTATTTCCAATTTTTCAGAGTTAGAGTTCCTTGGTGGTCTTCCATCTCTACAAAGCTTGTGGCTGGAAGGGAATCCATTATGTTGTGCCAGTTGGTATCGGCCACAAGTATTCAGCTATTTCTATAATCCAGAAAAATTGAAGTTAGATGATAAGGAAATCAGCACCAGAGAGTTTTGGAAGAGGCAACTAATTATTGCAAGTAGACAGAAGCGACCTGCCAGCTTTGGGTTTTATTCTCCCATAAAGTGTGATGATAAAGGAGATGCAAGTATCAACAGAAGAAGGAAAAAGGTGTCTCGTCTTGCTTCCATTGTAAGTGAAGAAGGGAGCACATCTCTCTGTTCTGATCAGGAGTCTGTGTCTTGTGACAATGAGATTCAAAGCAGAGAAGAACTTGTTATATCAGATGAGGAAGCTGAAATTGTTGATTTGATGACTAGAGTTGAGCAAATGAAGAAAGAACGCTCTGTTCTTTGGTTGCGAGAGTTCAAAGAATGGTTGGATCATGCTCCTGAGGATTCTGTGGACAGCAATAGACATAGCGGGACAACATTGCATATTGGCAGGGAAAATTATATAAAAGAAAAGTCAAGCTGGAGGCAGCTTGGTGAAAGCTCCAGATTTGTTTCAGACTATGCTCAAGCATCAGGAGATGAGAGTAGTACTAATGTTTTAGATTCAGATAGTTCCTTTATGGATATGTCTACTGGTTTGCAGGCCCACCACTTTGATAAAACTGGTTCCTTGGGAAATGCTGGTTTTGTCATCCCAGTTGGCATGGATAGAAGAGATCTGAAGGAAACCCTAAAAGATAGTTCACGTGAAGGAACAAGTAGTGTCCCTTTACAAGCAAAAAGTTTACATGGTCACATATTCACCGGTCAAAGAGGTCATAGGATGGTGGAAAATTTGAGTATGTCATTGACTTCTATAGATGATATTTCTGAATCTCGCTCCTCGTCTGTGTGCCCGGGATCACCACCTCATTATCAAAAGGACATTCTCAATCGTCGTCATAACTTGGAGGAAGAAATTTTACAGTTATCTGCAGAGTCATTTTCAGTGGCATCATCTGATAGTAATACCAGCTGTAGTGAAGACAATAACTATGAGTCTAGGCAGTCCATTCCTGAAGGTCATCAGCTACTGAATAAAAATATGGAAGAACATCCGTCTTCAGATCCTTTTAGGTTGTATGACATGAGATATGAGGTTCCGCATGTAAGAGGAAATGACAGGTGTTCAGTTGGAATAGGGGCAGAGAAAATTTCAAATTCAGACCTATCGCTGCAATCACATGCCAGTGTTGTTCCTGGTCGTGCTCATCATGGTGAGATTGCTCATTTTGTTGATGAAGAAGGTGATTTGTTAGAGAGGAGAAAACGCAGACAGAAAATAAAAAGAAGAGTTGTTACACTACTAGAAGATGACAAAATGGTTAGGCAAGCAGAAACATTACCAACATTAAATGGTAATATAGAGAATCATGTAACAATACTGGAAGATGAGCAAGATAGTCAATCTTTCTATGGGATTAACCTTGATGAAGTTATTGGTAAGAACCAGATGTTGACAAATACAAGTAATATCCCACTAATAAATGATACTACTGGATCTTCTGGGGCTGAATGTTTCTCTTCAAAGAGTGATGAATTTATTGAGGACTATTTCAGAAAGAATGTTGCAGATATGGGAAATAATGAAATTTGCAAGCAGTATATGCGCTGCTATTGTATACTTGAGTTGGATTCTCTATACAGAGAAAGAGAAGTAGCTGTCTTGCTGAGCAGTGAAAACAAAGTGTATGTGCTACTAATTGGTATTGGAGGTGATGGATCAGGCACTATCTTGAATTTGCAGGGTTGTCATAGGGTTGAAGACATAAGAGACGTTGTAGTTGGTGTAGGACTTCAGGTTGTGAGGGTGTATGTCGAGGAGAGCGCTGCCTACCTCTTTAAAACCAGAAGCATTGAAAGTTCAAGACAGTTACTTTCAACTTTAACAGTTATTGATTCTTATTCTCCAATCGATAAATTTTGTTTGAGAAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_4

Protein Analysis

952

Amino Acids

107.29

Weight (kDa)

5.81

Isoelectric Point (pI)

58.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_4 PF12799 192 - 231 2.9e-06 Leucine Rich repeats (2 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 772
AatII GACGTC 1 cut(s) 263
AbsI CCTCGAGG 1 cut(s) 134
Acc36I ACCTGC 1 cut(s) 1030
Acc65I GGTACC 1 cut(s) 24
AccB1I GGYRCC 2 cut(s) 24, 501
AccB7I CCANNNNNTGG 1 cut(s) 49
AccI GTMKAC 4 cut(s) 180, 1009, 1511, 2542
AccII CGCG 2 cut(s) 222, 383
AciI CCGC 7 cut(s) 130, 220, 311, 381, 451, 1350, 2024
AclWI GGATC 5 cut(s) 1317, 1789, 1982, 2428, 2650
AcoI YGGCCR 6 cut(s) 105, 294, 323, 378, 573, 905
AcsI RAATTY 8 cut(s) 1714, 1839, 2073, 2080, 2459, 2511, 2659, 2840
AcuI CTGAAG 5 cut(s) 113, 1622, 1959, 1968, 2702
AcvI CACGTG 1 cut(s) 1628
AcyI GRCGYC 1 cut(s) 260
AdeI CACNNNGTG 1 cut(s) 49
AfaI GTAC 2 cut(s) 26, 1471
AfeI AGCGCT 1 cut(s) 2749
AfiI CCNNNNNNNGG 5 cut(s) 21, 49, 136, 783, 1032
AflII CTTAAG 1 cut(s) 266
AgeI ACCGGT 2 cut(s) 504, 1685
AjiI CACGTC 1 cut(s) 375
AjnI CCWGG 2 cut(s) 27, 2119
Alw21I GWGCWC 3 cut(s) 153, 1139, 2131
Alw26I GTCTC 4 cut(s) 294, 758, 1107, 2688
AlwI GGATC 5 cut(s) 1317, 1789, 1982, 2428, 2650
AlwNI CAGNNNCTG 1 cut(s) 1954
Ama87I CYCGRG 5 cut(s) 134, 152, 227, 395, 1777
Aor51HI AGCGCT 1 cut(s) 2749
AoxI GGCC 8 cut(s) 105, 138, 294, 323, 378, 573, 905, 1524
ApeKI GCWGC 7 cut(s) 55, 232, 283, 1408, 2095, 2532, 2750
ApoI RAATTY 8 cut(s) 1714, 1839, 2073, 2080, 2459, 2511, 2659, 2840
AsiGI ACCGGT 2 cut(s) 504, 1685
Asp700I GAANNNNTTC 1 cut(s) 682
Asp718I GGTACC 1 cut(s) 24
AspLEI GCGC 4 cut(s) 173, 224, 2532, 2750
AspS9I GGNCC 1 cut(s) 1525
AsuC2I CCSGG 3 cut(s) 104, 1778, 1779
AsuHPI GGTGA 8 cut(s) 694, 707, 1427, 1675, 1776, 2150, 2180, 2648
AvaI CYCGRG 5 cut(s) 134, 152, 227, 395, 1777
AxyI CCTNAGG 1 cut(s) 1320
BaeGI GKGCMC 1 cut(s) 1778
BanI GGYRCC 2 cut(s) 24, 501
BanII GRGCYC 1 cut(s) 153
BarI GAAGNNNNNNTAC 4 cut(s) 1827, 1859, 2203, 2235
BauI CACGAG 1 cut(s) 146
BbrPI CACGTG 1 cut(s) 1628
BbsI GAAGAC 4 cut(s) 836, 1911, 1974, 2691
Bbv12I GWGCWC 3 cut(s) 153, 1139, 2131
BbvI GCAGC 7 cut(s) 67, 244, 270, 1420, 2082, 2519, 2737
BccI CCATC 4 cut(s) 541, 856, 1699, 2633
BceAI ACGGC 2 cut(s) 310, 560
BcgI CGANNNNNNTGC 2 cut(s) 373, 407
BciT130I CCWGG 2 cut(s) 29, 2121
BclI TGATCA 1 cut(s) 1153
BcnI CCSGG 3 cut(s) 104, 1778, 1779
BcoDI GTCTC 4 cut(s) 294, 758, 1107, 2688
BfaI CTAG 5 cut(s) 38, 653, 1245, 1923, 2228
BfmI CTRYAG 3 cut(s) 1737, 1854, 1897
BfoI RGCGCY 2 cut(s) 174, 2751
BfrI CTTAAG 1 cut(s) 266
BfuAI ACCTGC 1 cut(s) 1030
BglI GCCNNNNNGGC 1 cut(s) 499
BglII AGATCT 1 cut(s) 1597
BlpI GCTNAGC 1 cut(s) 2591
BmcAI AGTACT 1 cut(s) 1471
Bme1390I CCNGG 5 cut(s) 29, 104, 1778, 1779, 2121
BmeT110I CYCGRG 5 cut(s) 134, 152, 227, 395, 1777
BmgBI CACGTC 1 cut(s) 375
BmgT120I GGNCC 1 cut(s) 1525
BmiI GGNNCC 5 cut(s) 26, 291, 503, 1549, 2022
BmrFI CCNGG 5 cut(s) 29, 104, 1778, 1779, 2121
BmrI ACTGGG 1 cut(s) 1571
BmsI GCATC 5 cut(s) 350, 433, 1060, 1460, 1883
BmuI ACTGGG 1 cut(s) 1571
BpiI GAAGAC 4 cut(s) 836, 1911, 1974, 2691
BplI GAGNNNNNCTC 4 cut(s) 689, 721, 1126, 1158
BpmI CTGGAG 3 cut(s) 50, 1408, 1423
Bpu10I CCTNAGC 3 cut(s) 236, 338, 593
Bpu1102I GCTNAGC 1 cut(s) 2591
BpuEI CTTGAG 2 cut(s) 1431, 2567
BpuMI CCSGG 3 cut(s) 104, 1778, 1779
Bsa29I ATCGAT 1 cut(s) 2835
BsaAI YACGTR 1 cut(s) 1628
BsaHI GRCGYC 1 cut(s) 260
BsaI GGTCTC 1 cut(s) 294
BsaJI CCNNGG 6 cut(s) 208, 411, 658, 835, 1551, 1777
BsaWI WCCGGW 3 cut(s) 14, 504, 1685
BsaXI ACNNNNNCTCC 4 cut(s) 110, 140, 1151, 1181
Bsc4I CCNNNNNNNGG 5 cut(s) 21, 49, 136, 783, 1032
Bse118I RCCGGY 6 cut(s) 62, 107, 167, 286, 504, 1685
Bse1I ACTGG 7 cut(s) 894, 1519, 1549, 1577, 2109, 2310, 2422
Bse21I CCTNAGG 1 cut(s) 1320
Bse3DI GCAATG 1 cut(s) 1358
BseBI CCWGG 2 cut(s) 29, 2121
BseCI ATCGAT 1 cut(s) 2835
BseDI CCNNGG 6 cut(s) 208, 411, 658, 835, 1551, 1777
BseGI GGATG 5 cut(s) 365, 556, 1572, 1710, 1975
BseLI CCNNNNNNNGG 5 cut(s) 21, 49, 136, 783, 1032
BseMI GCAATG 1 cut(s) 1358
BseMII CTCAG 6 cut(s) 250, 262, 584, 665, 1311, 2582
BseNI ACTGG 7 cut(s) 894, 1519, 1549, 1577, 2109, 2310, 2422
BseRI GAGGAG 4 cut(s) 152, 1753, 2197, 2756
BseSI GKGCMC 1 cut(s) 1778
BseX3I CGGCCG 3 cut(s) 105, 378, 573
BseXI GCAGC 7 cut(s) 67, 244, 270, 1420, 2082, 2519, 2737
Bsh1236I CGCG 2 cut(s) 222, 383
Bsh1285I CGRYCG 3 cut(s) 108, 381, 576
BshFI GGCC 8 cut(s) 107, 140, 296, 325, 380, 575, 907, 1526
BshNI GGYRCC 2 cut(s) 24, 501
BshTI ACCGGT 2 cut(s) 504, 1685
BshVI ATCGAT 1 cut(s) 2835
BsiEI CGRYCG 3 cut(s) 108, 381, 576
BsiHKAI GWGCWC 3 cut(s) 153, 1139, 2131
BsiHKCI CYCGRG 5 cut(s) 134, 152, 227, 395, 1777
BslFI GGGAC 3 cut(s) 760, 1366, 1631
BslI CCNNNNNNNGG 5 cut(s) 21, 49, 136, 783, 1032
BsmAI GTCTC 4 cut(s) 294, 758, 1107, 2688
BsmBI CGTCTC 1 cut(s) 2688
BsmFI GGGAC 3 cut(s) 760, 1366, 1631
BsnI GGCC 8 cut(s) 107, 140, 296, 325, 380, 575, 907, 1526
Bso31I GGTCTC 1 cut(s) 294
BsoBI CYCGRG 5 cut(s) 134, 152, 227, 395, 1777
Bsp1286I GDGCHC 4 cut(s) 153, 1139, 1778, 2131
Bsp1720I GCTNAGC 1 cut(s) 2591
BspACI CCGC 7 cut(s) 130, 220, 311, 381, 451, 1350, 2024
BspANI GGCC 8 cut(s) 107, 140, 296, 325, 380, 575, 907, 1526
BspCNI CTCAG 6 cut(s) 249, 261, 585, 666, 1312, 2583
BspDI ATCGAT 1 cut(s) 2835
BspFNI CGCG 2 cut(s) 222, 383
BspLI GGNNCC 5 cut(s) 26, 291, 503, 1549, 2022
BspMAI CTGCAG 1 cut(s) 1858
BspMI ACCTGC 1 cut(s) 1030
BspPI GGATC 5 cut(s) 1317, 1789, 1982, 2428, 2650
BspT107I GGYRCC 2 cut(s) 24, 501
BspTI CTTAAG 1 cut(s) 266
BspTNI GGTCTC 1 cut(s) 294
BsrDI GCAATG 1 cut(s) 1358
BsrFI RCCGGY 6 cut(s) 62, 107, 167, 286, 504, 1685
BsrI ACTGG 7 cut(s) 894, 1519, 1549, 1577, 2109, 2310, 2422
BssAI RCCGGY 6 cut(s) 62, 107, 167, 286, 504, 1685
BssECI CCNNGG 6 cut(s) 208, 411, 658, 835, 1551, 1777
BssNAI GTATAC 1 cut(s) 2543
BssNI GRCGYC 1 cut(s) 260
BssSI CACGAG 1 cut(s) 146
BssT1I CCWWGG 4 cut(s) 411, 658, 835, 1551
Bst1107I GTATAC 1 cut(s) 2543
Bst2BI CACGAG 1 cut(s) 146
Bst2UI CCWGG 2 cut(s) 29, 2121
Bst4CI ACNGT 4 cut(s) 80, 1848, 2793, 2812
Bst6I CTCTTC 4 cut(s) 980, 1588, 2206, 2449
BstACI GRCGYC 1 cut(s) 260
BstAFI CTTAAG 1 cut(s) 266
BstAPI GCANNNNNTGC 1 cut(s) 2527
BstBAI YACGTR 1 cut(s) 1628
BstC8I GCNNGC 8 cut(s) 60, 109, 169, 288, 1027, 1524, 2253, 2519
BstDEI CTNAG 7 cut(s) 236, 248, 338, 593, 674, 1320, 2591
BstEII GGTNACC 1 cut(s) 700
BstF5I GGATG 5 cut(s) 365, 556, 1572, 1710, 1975
BstFNI CGCG 2 cut(s) 222, 383
BstH2I RGCGCY 2 cut(s) 174, 2751
BstHHI GCGC 4 cut(s) 173, 224, 2532, 2750
BstMAI GTCTC 4 cut(s) 294, 758, 1107, 2688
BstMCI CGRYCG 3 cut(s) 108, 381, 576
BstMWI GCNNNNNNNGC 4 cut(s) 219, 499, 636, 2527
BstNI CCWGG 2 cut(s) 29, 2121
BstNSI RCATGY 3 cut(s) 458, 2030, 2108
BstPI GGTNACC 1 cut(s) 700
BstSCI CCNGG 5 cut(s) 27, 102, 1776, 1777, 2119
BstSFI CTRYAG 3 cut(s) 1737, 1854, 1897
BstSLI GKGCMC 1 cut(s) 1778
BstUI CGCG 2 cut(s) 222, 383
BstV1I GCAGC 7 cut(s) 67, 244, 270, 1420, 2082, 2519, 2737
BstV2I GAAGAC 4 cut(s) 836, 1911, 1974, 2691
BstX2I RGATCY 3 cut(s) 1597, 1987, 2420
BstYI RGATCY 3 cut(s) 1597, 1987, 2420
BstZ17I GTATAC 1 cut(s) 2543
BstZI CGGCCG 3 cut(s) 105, 378, 573
Bsu15I ATCGAT 1 cut(s) 2835
Bsu36I CCTNAGG 1 cut(s) 1320
BsuRI GGCC 8 cut(s) 107, 140, 296, 325, 380, 575, 907, 1526
BsuTUI ATCGAT 1 cut(s) 2835
BtrI CACGTC 1 cut(s) 375
BtsCI GGATG 5 cut(s) 365, 556, 1572, 1710, 1975
BtsI GCAGTG 1 cut(s) 2602
BtsIMutI CAGTG 3 cut(s) 1875, 2116, 2602
BveI ACCTGC 1 cut(s) 1030
Cac8I GCNNGC 8 cut(s) 60, 109, 169, 288, 1027, 1524, 2253, 2519
CaiI CAGNNNCTG 1 cut(s) 1954
CfoI GCGC 4 cut(s) 173, 224, 2532, 2750
Cfr10I RCCGGY 6 cut(s) 62, 107, 167, 286, 504, 1685
Cfr13I GGNCC 1 cut(s) 1525
Cfr9I CCCGGG 1 cut(s) 1777
ClaI ATCGAT 1 cut(s) 2835
Csp6I GTAC 2 cut(s) 25, 1470
CspAI ACCGGT 2 cut(s) 504, 1685
CspCI CAANNNNNGTGG 2 cut(s) 845, 880
CviAII CATG 9 cut(s) 455, 1313, 1585, 1670, 2008, 2027, 2105, 2135, 2294
CviQI GTAC 2 cut(s) 25, 1470
DdeI CTNAG 7 cut(s) 236, 248, 338, 593, 674, 1320, 2591
DraI TTTAAA 1 cut(s) 2764
DraIII CACNNNGTG 1 cut(s) 49
DrdI GACNNNNNNGTC 1 cut(s) 772
DseDI GACNNNNNNGTC 1 cut(s) 772
EaeI YGGCCR 6 cut(s) 105, 294, 323, 378, 573, 905
EagI CGGCCG 3 cut(s) 105, 378, 573
Eam1104I CTCTTC 4 cut(s) 980, 1588, 2206, 2449
EarI CTCTTC 4 cut(s) 980, 1588, 2206, 2449
Ecl136II GAGCTC 1 cut(s) 151
EclXI CGGCCG 3 cut(s) 105, 378, 573
Eco130I CCWWGG 4 cut(s) 411, 658, 835, 1551
Eco147I AGGCCT 1 cut(s) 140
Eco24I GRGCYC 1 cut(s) 153
Eco31I GGTCTC 1 cut(s) 294
Eco47III AGCGCT 1 cut(s) 2749
Eco52I CGGCCG 3 cut(s) 105, 378, 573
Eco53kI GAGCTC 1 cut(s) 151
Eco57I CTGAAG 5 cut(s) 113, 1622, 1959, 1968, 2702
Eco72I CACGTG 1 cut(s) 1628
Eco81I CCTNAGG 1 cut(s) 1320
Eco88I CYCGRG 5 cut(s) 134, 152, 227, 395, 1777
Eco91I GGTNACC 1 cut(s) 700
EcoICRI GAGCTC 1 cut(s) 151
EcoO65I GGTNACC 1 cut(s) 700
EcoRII CCWGG 2 cut(s) 27, 2119
EcoT14I CCWWGG 4 cut(s) 411, 658, 835, 1551
EcoT38I GRGCYC 1 cut(s) 153
ErhI CCWWGG 4 cut(s) 411, 658, 835, 1551
Esp3I CGTCTC 1 cut(s) 2688
FaeI CATG 9 cut(s) 458, 1316, 1588, 1673, 2011, 2030, 2108, 2138, 2297
FaqI GGGAC 3 cut(s) 760, 1366, 1631
FatI CATG 9 cut(s) 454, 1312, 1584, 1669, 2007, 2026, 2104, 2134, 2293
FauI CCCGC 2 cut(s) 137, 1343
FbaI TGATCA 1 cut(s) 1153
FblI GTMKAC 4 cut(s) 180, 1009, 1511, 2542
FokI GGATG 5 cut(s) 372, 563, 1559, 1717, 1962
FriOI GRGCYC 1 cut(s) 153
FspBI CTAG 5 cut(s) 38, 653, 1245, 1923, 2228
GlaI GCGC 4 cut(s) 172, 223, 2531, 2749
GsuI CTGGAG 3 cut(s) 50, 1408, 1423
HaeII RGCGCY 2 cut(s) 174, 2751
HaeIII GGCC 8 cut(s) 107, 140, 296, 325, 380, 575, 907, 1526
HhaI GCGC 4 cut(s) 173, 224, 2532, 2750
Hin1I GRCGYC 1 cut(s) 260
Hin1II CATG 9 cut(s) 458, 1316, 1588, 1673, 2011, 2030, 2108, 2138, 2297
Hin6I GCGC 4 cut(s) 171, 222, 2530, 2748
HinP1I GCGC 4 cut(s) 171, 222, 2530, 2748
HincII GTYRAC 3 cut(s) 181, 258, 2379
HindII GTYRAC 3 cut(s) 181, 258, 2379
HindIII AAGCTT 1 cut(s) 859
HphI GGTGA 8 cut(s) 694, 707, 1427, 1675, 1776, 2150, 2180, 2648
Hpy99I CGWCG 5 cut(s) 80, 182, 265, 379, 1821
HpyAV CCTTC 9 cut(s) 776, 866, 1083, 1124, 1597, 1625, 1934, 2159, 2847
HpyCH4III ACNGT 4 cut(s) 80, 1848, 2793, 2812
HpyCH4IV ACGT 6 cut(s) 120, 260, 374, 749, 1627, 2697
HpyF10VI GCNNNNNNNGC 4 cut(s) 219, 499, 636, 2527
HpyF3I CTNAG 7 cut(s) 236, 248, 338, 593, 674, 1320, 2591
HpySE526I ACGT 6 cut(s) 120, 260, 374, 749, 1627, 2697
Hsp92I GRCGYC 1 cut(s) 260
Hsp92II CATG 9 cut(s) 458, 1316, 1588, 1673, 2011, 2030, 2108, 2138, 2297
HspAI GCGC 4 cut(s) 171, 222, 2530, 2748
KpnI GGTACC 1 cut(s) 28
KroI GCCGGC 3 cut(s) 107, 167, 286
KroNI GCCGGC 3 cut(s) 109, 169, 288
Ksp22I TGATCA 1 cut(s) 1153
LmnI GCTCC 8 cut(s) 52, 178, 295, 349, 1134, 1321, 1427, 1766
Lsp1109I GCAGC 7 cut(s) 67, 244, 270, 1420, 2082, 2519, 2737
LweI GCATC 5 cut(s) 350, 433, 1060, 1460, 1883
MaeI CTAG 5 cut(s) 38, 653, 1245, 1923, 2228
MaeII ACGT 6 cut(s) 120, 260, 374, 749, 1627, 2697
MaeIII GTNAC 8 cut(s) 10, 428, 700, 1172, 1673, 2218, 2296, 2793
MflI RGATCY 3 cut(s) 1597, 1987, 2420
MhlI GDGCHC 4 cut(s) 153, 1139, 1778, 2131
MlyI GAGTC 5 cut(s) 482, 563, 1169, 1868, 1928
MmeI TCCRAC 4 cut(s) 228, 1287, 2035, 2532
MreI CGCCGGCG 1 cut(s) 167
MroNI GCCGGC 3 cut(s) 107, 167, 286
MroXI GAANNNNTTC 1 cut(s) 682
MseI TTAA 6 cut(s) 267, 717, 2273, 2343, 2763, 2807
MslI CAYNNNNRTG 2 cut(s) 2109, 2136
MspA1I CMGCKG 2 cut(s) 313, 1896
MspCI CTTAAG 1 cut(s) 266
MspR9I CCNGG 5 cut(s) 29, 104, 1778, 1779, 2121
MvaI CCWGG 2 cut(s) 29, 2121
MvnI CGCG 2 cut(s) 222, 383
MwoI GCNNNNNNNGC 4 cut(s) 219, 499, 636, 2527
NaeI GCCGGC 3 cut(s) 109, 169, 288
NciI CCSGG 3 cut(s) 104, 1778, 1779
NgoMIV GCCGGC 3 cut(s) 107, 167, 286
NlaIII CATG 9 cut(s) 458, 1316, 1588, 1673, 2011, 2030, 2108, 2138, 2297
NlaIV GGNNCC 5 cut(s) 26, 291, 503, 1549, 2022
NmeAIII GCCGAG 1 cut(s) 189
NmuCI GTSAC 5 cut(s) 10, 428, 700, 1172, 1673
NspI RCATGY 3 cut(s) 458, 2030, 2108
PaeR7I CTCGAG 4 cut(s) 134, 152, 227, 395
PceI AGGCCT 1 cut(s) 140
PcsI WCGNNNNNNNCGW 3 cut(s) 314, 323, 380
PdiI GCCGGC 3 cut(s) 109, 169, 288
PdmI GAANNNNTTC 1 cut(s) 682
PfeI GAWTC 9 cut(s) 244, 877, 1183, 1325, 1484, 1754, 2290, 2555, 2819
PflMI CCANNNNNTGG 1 cut(s) 49
PinAI ACCGGT 2 cut(s) 504, 1685
PleI GAGTC 5 cut(s) 482, 562, 1168, 1867, 1927
PmaCI CACGTG 1 cut(s) 1628
PmlI CACGTG 1 cut(s) 1628
PpsI GAGTC 5 cut(s) 482, 562, 1168, 1867, 1927
Ppu21I YACGTR 1 cut(s) 1628
Psp124BI GAGCTC 1 cut(s) 153
Psp6I CCWGG 2 cut(s) 27, 2119
PspCI CACGTG 1 cut(s) 1628
PspEI GGTNACC 1 cut(s) 700
PspGI CCWGG 2 cut(s) 27, 2119
PspN4I GGNNCC 5 cut(s) 26, 291, 503, 1549, 2022
PspPI GGNCC 1 cut(s) 1525
PspXI VCTCGAGB 3 cut(s) 134, 227, 395
PstI CTGCAG 1 cut(s) 1858
PstNI CAGNNNCTG 1 cut(s) 1954
PsuI RGATCY 3 cut(s) 1597, 1987, 2420
PvuII CAGCTG 1 cut(s) 1896
RsaI GTAC 2 cut(s) 26, 1471
RsaNI GTAC 2 cut(s) 25, 1470
RseI CAYNNNNRTG 2 cut(s) 2109, 2136
SacI GAGCTC 1 cut(s) 153
SalI GTCGAC 1 cut(s) 179
SaqAI TTAA 6 cut(s) 267, 717, 2273, 2343, 2763, 2807
Sau96I GGNCC 1 cut(s) 1525
ScaI AGTACT 1 cut(s) 1471
SchI GAGTC 5 cut(s) 482, 563, 1169, 1868, 1928
ScrFI CCNGG 5 cut(s) 29, 104, 1778, 1779, 2121
SduI GDGCHC 4 cut(s) 153, 1139, 1778, 2131
SfaNI GCATC 5 cut(s) 350, 433, 1060, 1460, 1883
SfcI CTRYAG 3 cut(s) 1737, 1854, 1897
Sfr274I CTCGAG 4 cut(s) 134, 152, 227, 395
SgrAI CRCCGGYG 1 cut(s) 167
SlaI CTCGAG 4 cut(s) 134, 152, 227, 395
SmaI CCCGGG 1 cut(s) 1779
SmiMI CAYNNNNRTG 2 cut(s) 2109, 2136
SmlI CTYRAG 7 cut(s) 134, 152, 227, 266, 395, 1446, 2546
SmoI CTYRAG 7 cut(s) 134, 152, 227, 266, 395, 1446, 2546
SseBI AGGCCT 1 cut(s) 140
SsiI CCGC 7 cut(s) 130, 220, 311, 381, 451, 1350, 2024
SspI AATATT 1 cut(s) 805
SspMI CTAG 5 cut(s) 38, 653, 1245, 1923, 2228
SstI GAGCTC 1 cut(s) 153
StuI AGGCCT 1 cut(s) 140
StyD4I CCNGG 5 cut(s) 27, 102, 1776, 1777, 2119
StyI CCWWGG 4 cut(s) 411, 658, 835, 1551
TaaI ACNGT 4 cut(s) 80, 1848, 2793, 2812
TaiI ACGT 6 cut(s) 123, 263, 377, 752, 1630, 2700
TaqI TCGA 9 cut(s) 75, 135, 153, 180, 228, 396, 709, 2739, 2835
TatI WGTACW 1 cut(s) 1469
TauI GCSGC 3 cut(s) 313, 383, 454
TfiI GAWTC 9 cut(s) 244, 877, 1183, 1325, 1484, 1754, 2290, 2555, 2819
Tru1I TTAA 6 cut(s) 267, 717, 2273, 2343, 2763, 2807
Tru9I TTAA 6 cut(s) 267, 717, 2273, 2343, 2763, 2807
TscAI CASTG 3 cut(s) 1875, 2116, 2602
TseFI GTSAC 5 cut(s) 10, 428, 700, 1172, 1673
TseI GCWGC 7 cut(s) 55, 232, 283, 1408, 2095, 2532, 2750
Tsp45I GTSAC 5 cut(s) 10, 428, 700, 1172, 1673
TspDTI ATGAA 5 cut(s) 1274, 2175, 2367, 2472, 2523
TspGWI ACGGA 1 cut(s) 1968
TspMI CCCGGG 1 cut(s) 1777
TspRI CASTG 3 cut(s) 1875, 2116, 2602
Van91I CCANNNNNTGG 1 cut(s) 49
Vha464I CTTAAG 1 cut(s) 266
XapI RAATTY 8 cut(s) 1714, 1839, 2073, 2080, 2459, 2511, 2659, 2840
XceI RCATGY 3 cut(s) 458, 2030, 2108
XhoI CTCGAG 4 cut(s) 134, 152, 227, 395
XmaI CCCGGG 1 cut(s) 1777
XmiI GTMKAC 4 cut(s) 180, 1009, 1511, 2542
XmnI GAANNNNTTC 1 cut(s) 682
XspI CTAG 5 cut(s) 38, 653, 1245, 1923, 2228
ZraI GACGTC 1 cut(s) 261
ZrmI AGTACT 1 cut(s) 1471
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.