FvH4_5g18300

ADP-ribosylation factor GTPase-activating protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0010387
Physical Location & Seq
Reverse (-)
10531916 .. 10536985
5070 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g18300.t1

Sequence Viewer

Length: 729 bp
ATGAACAAGGCTTCCGTTTCCAAGGAGCTCGATGCCAAGCACGCCAAGATTTTAGAAGGTCTTCTTAAGCTGCCGGAAAACCGGGAATGTGCTGATTGTCGGAGCAAAGCCCCACGATGGGCAAGCGTGAACCTGGGAATATTTATTTGCATGCAATGTTCAGGAATCCATCGGAGCCTTGGAGTGCACATTTCAAAGGTCCGGTCTACTACTTTGGATACATGGCTGCCAGAGCAGATTGCTTTTATGGAATCTTTGGGTAATGAAAAGGCAAACCATTATTGGGAAAAAGAGTTGCCACCAAATGAAGACAGAAGTGAGATTGAGAAGTTTATTCGTGCCAAGTATGAGGGGAAAAAGTGGGTTGCGAAGAATGCAAAACAGCCCGCTCCTGAACTAGTTGAAATGGGCACCAGTTCCAACAAGTTGGTAGAAGGTGGAGCCACCAGTGGGAAGAAACTAAGGAGGCATTCTCTTGAGGAAGCAGTACTTCCTACGCGTCTTGCAGAAGATGCTCCTCCAGTAGCAAGACCCCGCACGGTTTCTTTAGATTTGAATCACACGTTGACTAGTTCACCTCTACCAAAGGGGCCAGCACCGATGACAGATTGTAATACAGCAACAACAAAGAGCAATGGTGAAACAAATCTCTTCAATTTGCTTCATGTCCATGATGCAAAACAGGAATGTGTAACTGTACCTCCTGCACGCTGGGCAACTTTCGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
ArfGap

Protein Analysis

243

Amino Acids

26.84

Weight (kDa)

8.69

Isoelectric Point (pI)

57.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ArfGap PF01412 16 - 124 4.8e-44 Putative GTPase activating protein for Arf
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 410
AccBSI CCGCTC 1 cut(s) 389
AccI GTMKAC 1 cut(s) 206
AccII CGCG 1 cut(s) 499
AciI CCGC 2 cut(s) 387, 535
AfaI GTAC 2 cut(s) 489, 699
AfiI CCNNNNNNNGG 4 cut(s) 117, 118, 283, 450
AflII CTTAAG 1 cut(s) 65
AflIII ACRYGT 2 cut(s) 497, 561
AgsI TTSAA 4 cut(s) 195, 404, 556, 655
AhlI ACTAGT 2 cut(s) 397, 569
AjnI CCWGG 1 cut(s) 132
AloI GAACNNNNNNTCC 1 cut(s) 28
AluBI AGCT 2 cut(s) 28, 70
AluI AGCT 2 cut(s) 28, 70
Alw21I GWGCWC 2 cut(s) 30, 189
Alw44I GTGCAC 1 cut(s) 185
AoxI GGCC 1 cut(s) 590
ApaLI GTGCAC 1 cut(s) 185
ApeKI GCWGC 2 cut(s) 70, 226
ArsI GACNNNNNNTTYG 2 cut(s) 188, 220
Asp700I GAANNNNTTC 1 cut(s) 60
AspS9I GGNCC 2 cut(s) 199, 590
AsuC2I CCSGG 1 cut(s) 83
AsuHPI GGTGA 2 cut(s) 567, 650
AvaII GGWCC 1 cut(s) 199
BaeGI GKGCMC 2 cut(s) 189, 413
BanI GGYRCC 1 cut(s) 410
BanII GRGCYC 1 cut(s) 30
BbsI GAAGAC 2 cut(s) 53, 315
Bbv12I GWGCWC 2 cut(s) 30, 189
BbvI GCAGC 2 cut(s) 57, 213
BccI CCATC 2 cut(s) 111, 177
BciT130I CCWGG 1 cut(s) 134
BciVI GTATCC 1 cut(s) 211
BcnI CCSGG 1 cut(s) 83
BcuI ACTAGT 2 cut(s) 397, 569
BfaI CTAG 2 cut(s) 398, 570
BfrI CTTAAG 1 cut(s) 65
BfuI GTATCC 1 cut(s) 211
BisI GCNGC 2 cut(s) 71, 227
BlsI GCNGC 2 cut(s) 72, 228
BmcAI AGTACT 1 cut(s) 489
Bme1390I CCNGG 2 cut(s) 83, 134
Bme18I GGWCC 1 cut(s) 199
BmgT120I GGNCC 2 cut(s) 199, 590
BmiI GGNNCC 4 cut(s) 176, 412, 442, 591
BmrFI CCNGG 2 cut(s) 83, 134
BmsI GCATC 3 cut(s) 22, 502, 664
BpiI GAAGAC 2 cut(s) 53, 315
BplI GAGNNNNNCTC 2 cut(s) 457, 489
BpmI CTGGAG 1 cut(s) 504
BpuEI CTTGAG 1 cut(s) 497
BpuMI CCSGG 1 cut(s) 83
BsaBI GATNNNNATC 1 cut(s) 555
BsaJI CCNNGG 3 cut(s) 21, 133, 178
BsaWI WCCGGW 1 cut(s) 201
BsaXI ACNNNNNCTCC 2 cut(s) 685, 715
Bsc4I CCNNNNNNNGG 4 cut(s) 117, 118, 283, 450
Bse1I ACTGG 3 cut(s) 414, 447, 521
Bse3DI GCAATG 2 cut(s) 161, 640
Bse8I GATNNNNATC 1 cut(s) 555
BseBI CCWGG 1 cut(s) 134
BseDI CCNNGG 3 cut(s) 21, 133, 178
BseJI GATNNNNATC 1 cut(s) 555
BseLI CCNNNNNNNGG 4 cut(s) 117, 118, 283, 450
BseMI GCAATG 2 cut(s) 161, 640
BseNI ACTGG 3 cut(s) 414, 447, 521
BseRI GAGGAG 1 cut(s) 507
BseSI GKGCMC 2 cut(s) 189, 413
BseXI GCAGC 2 cut(s) 57, 213
BseYI CCCAGC 1 cut(s) 711
BsgI GTGCAG 1 cut(s) 690
Bsh1236I CGCG 1 cut(s) 499
BshFI GGCC 1 cut(s) 592
BshNI GGYRCC 1 cut(s) 410
BsiHKAI GWGCWC 2 cut(s) 30, 189
BsiSI CCGG 3 cut(s) 74, 82, 202
BslI CCNNNNNNNGG 4 cut(s) 117, 118, 283, 450
BsmI GAATGC 2 cut(s) 379, 469
BsnI GGCC 1 cut(s) 592
Bsp1286I GDGCHC 3 cut(s) 30, 189, 413
BspACI CCGC 2 cut(s) 387, 535
BspANI GGCC 1 cut(s) 592
BspFNI CGCG 1 cut(s) 499
BspLI GGNNCC 4 cut(s) 176, 412, 442, 591
BspT107I GGYRCC 1 cut(s) 410
BspTI CTTAAG 1 cut(s) 65
BsrBI CCGCTC 1 cut(s) 389
BsrDI GCAATG 2 cut(s) 161, 640
BsrI ACTGG 3 cut(s) 414, 447, 521
BssECI CCNNGG 3 cut(s) 21, 133, 178
BssT1I CCWWGG 2 cut(s) 21, 178
Bst2UI CCWGG 1 cut(s) 134
Bst4CI ACNGT 2 cut(s) 541, 697
Bst6I CTCTTC 1 cut(s) 656
BstAFI CTTAAG 1 cut(s) 65
BstAPI GCANNNNNTGC 1 cut(s) 512
BstC8I GCNNGC 6 cut(s) 42, 124, 152, 387, 594, 709
BstDEI CTNAG 1 cut(s) 461
BstFNI CGCG 1 cut(s) 499
BstMWI GCNNNNNNNGC 5 cut(s) 41, 232, 374, 512, 713
BstNI CCWGG 1 cut(s) 134
BstNSI RCATGY 1 cut(s) 154
BstSCI CCNGG 2 cut(s) 81, 132
BstSLI GKGCMC 2 cut(s) 189, 413
BstUI CGCG 1 cut(s) 499
BstV1I GCAGC 2 cut(s) 57, 213
BstV2I GAAGAC 2 cut(s) 53, 315
BstXI CCANNNNNNTGG 1 cut(s) 427
BsuI GTATCC 1 cut(s) 211
BsuRI GGCC 1 cut(s) 592
BtsIMutI CAGTG 1 cut(s) 454
Cac8I GCNNGC 6 cut(s) 42, 124, 152, 387, 594, 709
Cfr13I GGNCC 2 cut(s) 199, 590
CseI GACGC 1 cut(s) 488
Csp6I GTAC 2 cut(s) 488, 698
CviAII CATG 4 cut(s) 151, 222, 665, 671
CviJI RGCY 9 cut(s) 11, 28, 70, 110, 177, 226, 385, 443, 592
CviKI_1 RGCY 9 cut(s) 11, 28, 70, 110, 177, 226, 385, 443, 592
CviQI GTAC 2 cut(s) 488, 698
DdeI CTNAG 1 cut(s) 461
Eam1104I CTCTTC 1 cut(s) 656
EarI CTCTTC 1 cut(s) 656
Ecl136II GAGCTC 1 cut(s) 28
Eco130I CCWWGG 2 cut(s) 21, 178
Eco24I GRGCYC 1 cut(s) 30
Eco47I GGWCC 1 cut(s) 199
Eco53kI GAGCTC 1 cut(s) 28
EcoICRI GAGCTC 1 cut(s) 28
EcoRII CCWGG 1 cut(s) 132
EcoT14I CCWWGG 2 cut(s) 21, 178
EcoT38I GRGCYC 1 cut(s) 30
ErhI CCWWGG 2 cut(s) 21, 178
FaeI CATG 4 cut(s) 154, 225, 668, 674
FaiI YATR 6 cut(s) 152, 223, 248, 348, 666, 672
FalI AAGNNNNNCTT 4 cut(s) 48, 80, 474, 506
FatI CATG 4 cut(s) 150, 221, 664, 670
FauI CCCGC 2 cut(s) 394, 542
FblI GTMKAC 1 cut(s) 206
Fnu4HI GCNGC 2 cut(s) 71, 227
FriOI GRGCYC 1 cut(s) 30
Fsp4HI GCNGC 2 cut(s) 71, 227
FspBI CTAG 2 cut(s) 398, 570
GluI GCNGC 2 cut(s) 71, 227
GsaI CCCAGC 1 cut(s) 715
GsuI CTGGAG 1 cut(s) 504
HaeIII GGCC 1 cut(s) 592
HapII CCGG 3 cut(s) 74, 82, 202
HgaI GACGC 1 cut(s) 488
Hin1II CATG 4 cut(s) 154, 225, 668, 674
HincII GTYRAC 1 cut(s) 567
HindII GTYRAC 1 cut(s) 567
HinfI GANTC 3 cut(s) 165, 251, 556
HpaII CCGG 3 cut(s) 74, 82, 202
HphI GGTGA 2 cut(s) 567, 650
Hpy166II GTNNAC 5 cut(s) 130, 187, 207, 567, 575
Hpy188I TCNGA 2 cut(s) 102, 174
Hpy188III TCNNGA 3 cut(s) 162, 392, 476
Hpy8I GTNNAC 5 cut(s) 130, 187, 207, 567, 575
HpyAV CCTTC 2 cut(s) 50, 428
HpyCH4III ACNGT 2 cut(s) 541, 697
HpyCH4IV ACGT 1 cut(s) 563
HpyCH4V TGCA 7 cut(s) 150, 154, 187, 377, 506, 677, 707
HpyF10VI GCNNNNNNNGC 5 cut(s) 41, 232, 374, 512, 713
HpyF3I CTNAG 1 cut(s) 461
HpySE526I ACGT 1 cut(s) 563
Hsp92II CATG 4 cut(s) 154, 225, 668, 674
LmnI GCTCC 6 cut(s) 25, 102, 174, 394, 440, 520
Lsp1109I GCAGC 2 cut(s) 57, 213
LweI GCATC 3 cut(s) 22, 502, 664
MaeI CTAG 2 cut(s) 398, 570
MaeII ACGT 1 cut(s) 563
MaeIII GTNAC 1 cut(s) 691
MbiI CCGCTC 1 cut(s) 389
MboII GAAGA 6 cut(s) 53, 320, 382, 466, 521, 643
MhlI GDGCHC 3 cut(s) 30, 189, 413
MluCI AATT 1 cut(s) 655
MluI ACGCGT 1 cut(s) 497
MmeI TCCRAC 2 cut(s) 80, 444
MnlI CCTC 6 cut(s) 343, 459, 472, 528, 588, 711
MroXI GAANNNNTTC 1 cut(s) 60
MseI TTAA 1 cut(s) 66
MslI CAYNNNNRTG 1 cut(s) 669
MspCI CTTAAG 1 cut(s) 65
MspI CCGG 3 cut(s) 74, 82, 202
MspR9I CCNGG 2 cut(s) 83, 134
Mva1269I GAATGC 2 cut(s) 379, 469
MvaI CCWGG 1 cut(s) 134
MvnI CGCG 1 cut(s) 499
MwoI GCNNNNNNNGC 5 cut(s) 41, 232, 374, 512, 713
NciI CCSGG 1 cut(s) 83
NlaIII CATG 4 cut(s) 154, 225, 668, 674
NlaIV GGNNCC 4 cut(s) 176, 412, 442, 591
NspI RCATGY 1 cut(s) 154
PaeI GCATGC 1 cut(s) 154
PctI GAATGC 2 cut(s) 379, 469
PdmI GAANNNNTTC 1 cut(s) 60
PfeI GAWTC 3 cut(s) 165, 251, 556
PkrI GCNGC 2 cut(s) 72, 228
Psp124BI GAGCTC 1 cut(s) 30
Psp6I CCWGG 1 cut(s) 132
PspFI CCCAGC 1 cut(s) 711
PspGI CCWGG 1 cut(s) 132
PspN4I GGNNCC 4 cut(s) 176, 412, 442, 591
PspPI GGNCC 2 cut(s) 199, 590
RsaI GTAC 2 cut(s) 489, 699
RsaNI GTAC 2 cut(s) 488, 698
RseI CAYNNNNRTG 1 cut(s) 669
SacI GAGCTC 1 cut(s) 30
SaqAI TTAA 1 cut(s) 66
SatI GCNGC 2 cut(s) 71, 227
Sau96I GGNCC 2 cut(s) 199, 590
ScaI AGTACT 1 cut(s) 489
ScrFI CCNGG 2 cut(s) 83, 134
SduI GDGCHC 3 cut(s) 30, 189, 413
SetI ASST 9 cut(s) 30, 61, 72, 135, 201, 439, 566, 580, 703
SfaNI GCATC 3 cut(s) 22, 502, 664
SinI GGWCC 1 cut(s) 199
SmiMI CAYNNNNRTG 1 cut(s) 669
SmlI CTYRAG 2 cut(s) 65, 476
SmoI CTYRAG 2 cut(s) 65, 476
SpeI ACTAGT 2 cut(s) 397, 569
SphI GCATGC 1 cut(s) 154
Sse9I AATT 1 cut(s) 655
SsiI CCGC 2 cut(s) 387, 535
SspI AATATT 1 cut(s) 141
SspMI CTAG 2 cut(s) 398, 570
SstI GAGCTC 1 cut(s) 30
StyD4I CCNGG 2 cut(s) 81, 132
StyI CCWWGG 2 cut(s) 21, 178
TaaI ACNGT 2 cut(s) 541, 697
TaiI ACGT 1 cut(s) 566
TaqI TCGA 2 cut(s) 30, 723
TasI AATT 1 cut(s) 655
TatI WGTACW 1 cut(s) 487
TfiI GAWTC 3 cut(s) 165, 251, 556
Tru1I TTAA 1 cut(s) 66
Tru9I TTAA 1 cut(s) 66
TscAI CASTG 1 cut(s) 454
TseI GCWGC 2 cut(s) 70, 226
TspDTI ATGAA 4 cut(s) 17, 279, 321, 653
TspGWI ACGGA 1 cut(s) 4
TspRI CASTG 1 cut(s) 454
Vha464I CTTAAG 1 cut(s) 65
VneI GTGCAC 1 cut(s) 185
VpaK11BI GGWCC 1 cut(s) 199
XceI RCATGY 1 cut(s) 154
XcmI CCANNNNNNNNNTGG 1 cut(s) 176
XmiI GTMKAC 1 cut(s) 206
XmnI GAANNNNTTC 1 cut(s) 60
XspI CTAG 2 cut(s) 398, 570
ZrmI AGTACT 1 cut(s) 489
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.