RLG00000005549

ADP-ribosylation factor GTPase-activating protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0010387
Physical Location & Seq
Reverse (-)
68487659 .. 68490709
3051 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005549

Sequence Viewer

Length: 648 bp
ATGAGCAAGGCCTCCATTTCCAAGGAGCTCAACGACAAGCACACCAAGATTTTAGAAGGTCTTCTTAAGCTGCCAGAAAACCGGGAATGTGCTGATTGTCGGAGCAAAGCCCCACGATGGGCAAGTGTGAATCTGGGAATATTTATTTGCATGCAATGTTCAGGAATTCATCGGAGCCTTGGAGTGCACATTTCAAAGGTCCGGTCTACTACTTTGGATACATGGCTGCCAGAGCAGATTGCTTTTATGGAATCTTTGGGTAATGAAAAGGCAAACAATTATTGGGAAAAAAAGTTGCCACTAAATGCAGACAGAAGTGCAATCGAGAAGTTTATTCGTGCCAAGTATGAGGGAAAAAAGTGGGTTGCAAAGGATGCAAAACAGCCCGCTCCTGAACCAGTTGAAATGGGAACCAGTTCCAACAAGTTGGTAGAAGGTGGAGCCACCAGTGGCAAGAAACTAAGGAGGCATTCTCTTGAGGAAGCAATACTTCCTACACATCTTTCAGAAGATGCTCCTCCATTAGCAAGACCCCGCACGGGTTCTTTAGATTTGAATCACATTTATACTAATTCACCTCTACCAAAGGGGAATGTCAAACTGTACCTCCTGCACGCTGGGCAACTTTTGATTGACAGGACTCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
ArfGap

Protein Analysis

216

Amino Acids

23.97

Weight (kDa)

9.41

Isoelectric Point (pI)

50.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ArfGap PF01412 16 - 124 1.8e-43 Putative GTPase activating protein for Arf
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 389
AccI GTMKAC 1 cut(s) 206
AciI CCGC 2 cut(s) 387, 535
AcsI RAATTY 1 cut(s) 165
AfaI GTAC 1 cut(s) 605
AfiI CCNNNNNNNGG 3 cut(s) 117, 118, 539
AflII CTTAAG 1 cut(s) 65
AgsI TTSAA 3 cut(s) 195, 404, 556
AluBI AGCT 2 cut(s) 28, 70
AluI AGCT 2 cut(s) 28, 70
Alw21I GWGCWC 2 cut(s) 30, 189
Alw44I GTGCAC 1 cut(s) 185
AoxI GGCC 1 cut(s) 9
ApaLI GTGCAC 1 cut(s) 185
ApeKI GCWGC 2 cut(s) 70, 226
ApoI RAATTY 1 cut(s) 165
ArsI GACNNNNNNTTYG 2 cut(s) 188, 220
Asp700I GAANNNNTTC 2 cut(s) 60, 415
AspS9I GGNCC 1 cut(s) 199
AsuC2I CCSGG 1 cut(s) 83
AsuHPI GGTGA 1 cut(s) 567
AvaII GGWCC 1 cut(s) 199
BaeGI GKGCMC 1 cut(s) 189
BanII GRGCYC 1 cut(s) 30
BbsI GAAGAC 1 cut(s) 53
Bbv12I GWGCWC 2 cut(s) 30, 189
BbvI GCAGC 2 cut(s) 57, 213
BccI CCATC 1 cut(s) 111
BciVI GTATCC 1 cut(s) 211
BcnI CCSGG 1 cut(s) 83
BfrI CTTAAG 1 cut(s) 65
BfuI GTATCC 1 cut(s) 211
BisI GCNGC 2 cut(s) 71, 227
BlsI GCNGC 2 cut(s) 72, 228
Bme1390I CCNGG 1 cut(s) 83
Bme18I GGWCC 1 cut(s) 199
BmgT120I GGNCC 1 cut(s) 199
BmiI GGNNCC 3 cut(s) 176, 412, 442
BmrFI CCNGG 1 cut(s) 83
BmsI GCATC 2 cut(s) 364, 502
BpiI GAAGAC 1 cut(s) 53
BplI GAGNNNNNCTC 2 cut(s) 457, 489
BpuEI CTTGAG 1 cut(s) 497
BpuMI CCSGG 1 cut(s) 83
BsaBI GATNNNNATC 1 cut(s) 555
BsaJI CCNNGG 2 cut(s) 21, 178
BsaWI WCCGGW 1 cut(s) 201
BsaXI ACNNNNNCTCC 2 cut(s) 591, 621
Bsc4I CCNNNNNNNGG 3 cut(s) 117, 118, 539
Bse1I ACTGG 3 cut(s) 398, 414, 447
Bse3DI GCAATG 1 cut(s) 161
Bse8I GATNNNNATC 1 cut(s) 555
BseDI CCNNGG 2 cut(s) 21, 178
BseGI GGATG 1 cut(s) 379
BseJI GATNNNNATC 1 cut(s) 555
BseLI CCNNNNNNNGG 3 cut(s) 117, 118, 539
BseMI GCAATG 1 cut(s) 161
BseNI ACTGG 3 cut(s) 398, 414, 447
BseRI GAGGAG 1 cut(s) 507
BseSI GKGCMC 1 cut(s) 189
BseXI GCAGC 2 cut(s) 57, 213
BseYI CCCAGC 1 cut(s) 617
BsgI GTGCAG 1 cut(s) 596
BshFI GGCC 1 cut(s) 11
BsiHKAI GWGCWC 2 cut(s) 30, 189
BsiSI CCGG 2 cut(s) 82, 202
BslI CCNNNNNNNGG 3 cut(s) 117, 118, 539
BsmI GAATGC 1 cut(s) 469
BsnI GGCC 1 cut(s) 11
Bsp1286I GDGCHC 2 cut(s) 30, 189
BspACI CCGC 2 cut(s) 387, 535
BspANI GGCC 1 cut(s) 11
BspLI GGNNCC 3 cut(s) 176, 412, 442
BspTI CTTAAG 1 cut(s) 65
BsrBI CCGCTC 1 cut(s) 389
BsrDI GCAATG 1 cut(s) 161
BsrI ACTGG 3 cut(s) 398, 414, 447
BssECI CCNNGG 2 cut(s) 21, 178
BssT1I CCWWGG 2 cut(s) 21, 178
Bst4CI ACNGT 1 cut(s) 603
BstAFI CTTAAG 1 cut(s) 65
BstAPI GCANNNNNTGC 1 cut(s) 374
BstC8I GCNNGC 3 cut(s) 152, 387, 615
BstDEI CTNAG 1 cut(s) 461
BstF5I GGATG 1 cut(s) 379
BstMWI GCNNNNNNNGC 3 cut(s) 232, 374, 619
BstNSI RCATGY 1 cut(s) 154
BstSCI CCNGG 1 cut(s) 81
BstSLI GKGCMC 1 cut(s) 189
BstV1I GCAGC 2 cut(s) 57, 213
BstV2I GAAGAC 1 cut(s) 53
BstXI CCANNNNNNTGG 1 cut(s) 427
BsuI GTATCC 1 cut(s) 211
BsuRI GGCC 1 cut(s) 11
BtsCI GGATG 1 cut(s) 379
BtsIMutI CAGTG 1 cut(s) 454
Cac8I GCNNGC 3 cut(s) 152, 387, 615
Cfr13I GGNCC 1 cut(s) 199
Csp6I GTAC 1 cut(s) 604
CviAII CATG 2 cut(s) 151, 222
CviJI RGCY 8 cut(s) 11, 28, 70, 110, 177, 226, 385, 443
CviKI_1 RGCY 8 cut(s) 11, 28, 70, 110, 177, 226, 385, 443
CviQI GTAC 1 cut(s) 604
DdeI CTNAG 1 cut(s) 461
Ecl136II GAGCTC 1 cut(s) 28
Eco130I CCWWGG 2 cut(s) 21, 178
Eco147I AGGCCT 1 cut(s) 11
Eco24I GRGCYC 1 cut(s) 30
Eco47I GGWCC 1 cut(s) 199
Eco53kI GAGCTC 1 cut(s) 28
EcoICRI GAGCTC 1 cut(s) 28
EcoRI GAATTC 1 cut(s) 165
EcoT14I CCWWGG 2 cut(s) 21, 178
EcoT38I GRGCYC 1 cut(s) 30
ErhI CCWWGG 2 cut(s) 21, 178
FaeI CATG 2 cut(s) 154, 225
FaiI YATR 5 cut(s) 152, 223, 248, 348, 567
FalI AAGNNNNNCTT 4 cut(s) 48, 80, 474, 506
FatI CATG 2 cut(s) 150, 221
FauI CCCGC 2 cut(s) 394, 542
FblI GTMKAC 1 cut(s) 206
Fnu4HI GCNGC 2 cut(s) 71, 227
FokI GGATG 1 cut(s) 386
FriOI GRGCYC 1 cut(s) 30
Fsp4HI GCNGC 2 cut(s) 71, 227
GluI GCNGC 2 cut(s) 71, 227
GsaI CCCAGC 1 cut(s) 621
HaeIII GGCC 1 cut(s) 11
HapII CCGG 2 cut(s) 82, 202
Hin1II CATG 2 cut(s) 154, 225
HinfI GANTC 4 cut(s) 130, 251, 556, 640
HpaII CCGG 2 cut(s) 82, 202
HphI GGTGA 1 cut(s) 567
Hpy166II GTNNAC 2 cut(s) 187, 207
Hpy188I TCNGA 3 cut(s) 102, 174, 508
Hpy188III TCNNGA 4 cut(s) 162, 325, 392, 476
Hpy8I GTNNAC 2 cut(s) 187, 207
HpyAV CCTTC 2 cut(s) 50, 428
HpyCH4III ACNGT 1 cut(s) 603
HpyCH4V TGCA 8 cut(s) 150, 154, 187, 308, 320, 368, 377, 613
HpyF10VI GCNNNNNNNGC 3 cut(s) 232, 374, 619
HpyF3I CTNAG 1 cut(s) 461
Hsp92II CATG 2 cut(s) 154, 225
LmnI GCTCC 6 cut(s) 25, 102, 174, 394, 440, 520
Lsp1109I GCAGC 2 cut(s) 57, 213
LweI GCATC 2 cut(s) 364, 502
MbiI CCGCTC 1 cut(s) 389
MboII GAAGA 2 cut(s) 53, 521
MhlI GDGCHC 2 cut(s) 30, 189
MluCI AATT 3 cut(s) 165, 277, 571
MlyI GAGTC 1 cut(s) 634
MmeI TCCRAC 2 cut(s) 80, 444
MnlI CCTC 7 cut(s) 22, 343, 459, 472, 528, 588, 617
MroXI GAANNNNTTC 2 cut(s) 60, 415
MseI TTAA 1 cut(s) 66
MspCI CTTAAG 1 cut(s) 65
MspI CCGG 2 cut(s) 82, 202
MspR9I CCNGG 1 cut(s) 83
Mva1269I GAATGC 1 cut(s) 469
MwoI GCNNNNNNNGC 3 cut(s) 232, 374, 619
NciI CCSGG 1 cut(s) 83
NlaIII CATG 2 cut(s) 154, 225
NlaIV GGNNCC 3 cut(s) 176, 412, 442
NspI RCATGY 1 cut(s) 154
PaeI GCATGC 1 cut(s) 154
PceI AGGCCT 1 cut(s) 11
PctI GAATGC 1 cut(s) 469
PdmI GAANNNNTTC 2 cut(s) 60, 415
PfeI GAWTC 3 cut(s) 130, 251, 556
PkrI GCNGC 2 cut(s) 72, 228
PleI GAGTC 1 cut(s) 634
PpsI GAGTC 1 cut(s) 634
Psp124BI GAGCTC 1 cut(s) 30
PspFI CCCAGC 1 cut(s) 617
PspN4I GGNNCC 3 cut(s) 176, 412, 442
PspPI GGNCC 1 cut(s) 199
RsaI GTAC 1 cut(s) 605
RsaNI GTAC 1 cut(s) 604
SacI GAGCTC 1 cut(s) 30
SaqAI TTAA 1 cut(s) 66
SatI GCNGC 2 cut(s) 71, 227
Sau96I GGNCC 1 cut(s) 199
SchI GAGTC 1 cut(s) 634
ScrFI CCNGG 1 cut(s) 83
SduI GDGCHC 2 cut(s) 30, 189
SetI ASST 7 cut(s) 30, 61, 72, 201, 439, 580, 609
SfaNI GCATC 2 cut(s) 364, 502
SinI GGWCC 1 cut(s) 199
SmlI CTYRAG 2 cut(s) 65, 476
SmoI CTYRAG 2 cut(s) 65, 476
SphI GCATGC 1 cut(s) 154
Sse9I AATT 3 cut(s) 165, 277, 571
SseBI AGGCCT 1 cut(s) 11
SsiI CCGC 2 cut(s) 387, 535
SspI AATATT 1 cut(s) 141
SstI GAGCTC 1 cut(s) 30
StuI AGGCCT 1 cut(s) 11
StyD4I CCNGG 1 cut(s) 81
StyI CCWWGG 2 cut(s) 21, 178
TaaI ACNGT 1 cut(s) 603
TaqI TCGA 1 cut(s) 324
TasI AATT 3 cut(s) 165, 277, 571
TfiI GAWTC 3 cut(s) 130, 251, 556
Tru1I TTAA 1 cut(s) 66
Tru9I TTAA 1 cut(s) 66
TscAI CASTG 1 cut(s) 454
TseI GCWGC 2 cut(s) 70, 226
TspDTI ATGAA 2 cut(s) 158, 279
TspRI CASTG 1 cut(s) 454
Vha464I CTTAAG 1 cut(s) 65
VneI GTGCAC 1 cut(s) 185
VpaK11BI GGWCC 1 cut(s) 199
XapI RAATTY 1 cut(s) 165
XceI RCATGY 1 cut(s) 154
XmiI GTMKAC 1 cut(s) 206
XmnI GAANNNNTTC 2 cut(s) 60, 415
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.