MD06G1238800.v1.1

ADP-ribosylation factor GTPase-activating protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0010387
Physical Location & Seq
Reverse (-)
36961503 .. 36964705
3203 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1238800.v1.1.491

Sequence Viewer

Length: 783 bp
ATGAACAACAAGGCCTCCGTTTCCAAAGACCTCAACGCCAAACACCTCAAGGTTTTAGAAGGCCTTCTTAAGCTGCCACAAAACAGGGAATGTGCTGATTGCCGGAGCAAAGCCCCGCGATGGGCTAGTGTCAACCTGGGAATATTTATTTGCATGCAGTGCTCAGGAATTCATCGGAGTCTTGGAGTGCATATTTCAAAGGTACGGTCTACAACTTTGGATACATGGCTGCCAGAACAGGTTGCTTTTATGGAATCCATGGGTAATGAAAAGGCAAACAGTTATTGGGAAAAAGAATTGCCACCAAATTTTGACAGAAGTGGGACTGAGAAATTTATTCGTGCCAAGTATGAGGGGAAAATGTGGGTTTCGAAGAATGCAAAACAGCCAGCTCCTAAACCAGTAGATATGAGCATCAGTTCCAACAGTTTGGTAAAGGGCGGACCCACACGTGGAGTTCCTAAGAAAACAAGGAGACATTCTCTTGAGGAGGCAATGCTTTTTACGAACCTGGCAGAAGCTGCTCCTACAGTGGCAAGATCCCGTGGGGGTTCTTTAGATTTGAATCATAAGTTTAGTACGTCACCTCCAGCAAAGGGGCCACCACCAATGATGGAATGTGATACAGTCACAAAAAAGATCAACGGTACAACAGACCTATTCAGCTTGCTTTATGTTCATGATGCAAAGCAGAAGTATGCCACTCCTCCGCTCTGGCAAACTTTTGATTGTAAGAAAACTATAGAGAAGATAATTTTAAGTGAAGCCCATGGAATTCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
ArfGap

Protein Analysis

261

Amino Acids

28.95

Weight (kDa)

9.56

Isoelectric Point (pI)

50.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ArfGap PF01412 18 - 125 2.3e-42 Putative GTPase activating protein for Arf
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 712
AccI GTMKAC 1 cut(s) 209
AccII CGCG 1 cut(s) 118
AciI CCGC 3 cut(s) 116, 441, 710
AclWI GGATC 1 cut(s) 534
AcsI RAATTY 4 cut(s) 168, 307, 332, 774
AcvI CACGTG 1 cut(s) 452
AfaI GTAC 3 cut(s) 204, 580, 649
AfiI CCNNNNNNNGG 4 cut(s) 120, 121, 452, 596
AflII CTTAAG 1 cut(s) 68
AflIII ACRYGT 1 cut(s) 449
AgsI TTSAA 2 cut(s) 198, 565
AjnI CCWGG 2 cut(s) 135, 510
AluBI AGCT 4 cut(s) 73, 392, 521, 666
AluI AGCT 4 cut(s) 73, 392, 521, 666
Alw21I GWGCWC 1 cut(s) 164
Alw26I GTCTC 1 cut(s) 469
AlwI GGATC 1 cut(s) 534
AlwNI CAGNNNCTG 1 cut(s) 521
AoxI GGCC 3 cut(s) 12, 61, 599
ApeKI GCWGC 3 cut(s) 73, 229, 521
ApoI RAATTY 4 cut(s) 168, 307, 332, 774
ArsI GACNNNNNNTTYG 2 cut(s) 191, 223
Asp700I GAANNNNTTC 1 cut(s) 63
AspS9I GGNCC 2 cut(s) 443, 599
AsuHPI GGTGA 1 cut(s) 576
AsuII TTCGAA 1 cut(s) 371
AvaII GGWCC 1 cut(s) 443
BbrPI CACGTG 1 cut(s) 452
Bbv12I GWGCWC 1 cut(s) 164
BbvI GCAGC 3 cut(s) 60, 216, 508
BccI CCATC 2 cut(s) 114, 607
BciT130I CCWGG 2 cut(s) 137, 512
BciVI GTATCC 1 cut(s) 214
BcoDI GTCTC 1 cut(s) 469
BfaI CTAG 1 cut(s) 126
BfmI CTRYAG 2 cut(s) 528, 741
BfrI CTTAAG 1 cut(s) 68
BfuI GTATCC 1 cut(s) 214
BisI GCNGC 3 cut(s) 74, 230, 522
BlsI GCNGC 3 cut(s) 75, 231, 523
Bme1390I CCNGG 2 cut(s) 137, 512
Bme18I GGWCC 1 cut(s) 443
BmgT120I GGNCC 2 cut(s) 443, 599
BmiI GGNNCC 2 cut(s) 445, 600
BmrFI CCNGG 2 cut(s) 137, 512
BmsI GCATC 2 cut(s) 423, 673
BplI GAGNNNNNCTC 2 cut(s) 466, 498
BpmI CTGGAG 1 cut(s) 573
Bpu10I CCTNAGC 1 cut(s) 163
Bpu14I TTCGAA 1 cut(s) 371
BpuEI CTTGAG 2 cut(s) 32, 506
BsaAI YACGTR 1 cut(s) 452
BsaBI GATNNNNATC 1 cut(s) 564
BsaJI CCNNGG 4 cut(s) 136, 258, 544, 769
BsaXI ACNNNNNCTCC 3 cut(s) 29, 571, 601
Bsc4I CCNNNNNNNGG 4 cut(s) 120, 121, 452, 596
Bse1I ACTGG 1 cut(s) 401
Bse3DI GCAATG 1 cut(s) 501
Bse8I GATNNNNATC 1 cut(s) 564
BseBI CCWGG 2 cut(s) 137, 512
BseDI CCNNGG 4 cut(s) 136, 258, 544, 769
BseJI GATNNNNATC 1 cut(s) 564
BseLI CCNNNNNNNGG 4 cut(s) 120, 121, 452, 596
BseMI GCAATG 1 cut(s) 501
BseMII CTCAG 2 cut(s) 177, 318
BseNI ACTGG 1 cut(s) 401
BseRI GAGGAG 2 cut(s) 503, 696
BseXI GCAGC 3 cut(s) 60, 216, 508
Bsh1236I CGCG 1 cut(s) 118
BshFI GGCC 3 cut(s) 14, 63, 601
BsiHKAI GWGCWC 1 cut(s) 164
BsiSI CCGG 1 cut(s) 103
BslFI GGGAC 1 cut(s) 337
BslI CCNNNNNNNGG 4 cut(s) 120, 121, 452, 596
BsmAI GTCTC 1 cut(s) 469
BsmFI GGGAC 1 cut(s) 337
BsmI GAATGC 1 cut(s) 382
BsnI GGCC 3 cut(s) 14, 63, 601
Bsp119I TTCGAA 1 cut(s) 371
Bsp1286I GDGCHC 1 cut(s) 164
Bsp143I GATC 2 cut(s) 539, 639
Bsp19I CCATGG 2 cut(s) 258, 769
BspACI CCGC 3 cut(s) 116, 441, 710
BspANI GGCC 3 cut(s) 14, 63, 601
BspCNI CTCAG 2 cut(s) 176, 319
BspFNI CGCG 1 cut(s) 118
BspHI TCATGA 1 cut(s) 679
BspLI GGNNCC 2 cut(s) 445, 600
BspPI GGATC 1 cut(s) 534
BspT104I TTCGAA 1 cut(s) 371
BspTI CTTAAG 1 cut(s) 68
BsrBI CCGCTC 1 cut(s) 712
BsrDI GCAATG 1 cut(s) 501
BsrI ACTGG 1 cut(s) 401
BssECI CCNNGG 4 cut(s) 136, 258, 544, 769
BssMI GATC 2 cut(s) 539, 639
BssT1I CCWWGG 2 cut(s) 258, 769
Bst2UI CCWGG 2 cut(s) 137, 512
Bst4CI ACNGT 6 cut(s) 207, 281, 428, 532, 628, 647
BstAFI CTTAAG 1 cut(s) 68
BstAPI GCANNNNNTGC 2 cut(s) 159, 521
BstBAI YACGTR 1 cut(s) 452
BstBI TTCGAA 1 cut(s) 371
BstC8I GCNNGC 3 cut(s) 155, 390, 668
BstDEI CTNAG 3 cut(s) 163, 327, 462
BstDSI CCRYGG 3 cut(s) 258, 544, 769
BstFNI CGCG 1 cut(s) 118
BstKTI GATC 2 cut(s) 542, 642
BstMAI GTCTC 1 cut(s) 469
BstMBI GATC 2 cut(s) 539, 639
BstMWI GCNNNNNNNGC 2 cut(s) 159, 521
BstNI CCWGG 2 cut(s) 137, 512
BstNSI RCATGY 1 cut(s) 157
BstSCI CCNGG 2 cut(s) 135, 510
BstSFI CTRYAG 2 cut(s) 528, 741
BstUI CGCG 1 cut(s) 118
BstV1I GCAGC 3 cut(s) 60, 216, 508
BstX2I RGATCY 1 cut(s) 539
BstXI CCANNNNNNTGG 1 cut(s) 430
BstYI RGATCY 1 cut(s) 539
BsuI GTATCC 1 cut(s) 214
BsuRI GGCC 3 cut(s) 14, 63, 601
BtgI CCRYGG 3 cut(s) 258, 544, 769
BtgZI GCGATG 1 cut(s) 133
BtsI GCAGTG 1 cut(s) 164
BtsIMutI CAGTG 2 cut(s) 164, 537
Cac8I GCNNGC 3 cut(s) 155, 390, 668
CaiI CAGNNNCTG 1 cut(s) 521
CciI TCATGA 1 cut(s) 679
Cfr13I GGNCC 2 cut(s) 443, 599
Csp6I GTAC 3 cut(s) 203, 579, 648
CviAII CATG 5 cut(s) 154, 225, 259, 680, 770
CviQI GTAC 3 cut(s) 203, 579, 648
DdeI CTNAG 3 cut(s) 163, 327, 462
DpnI GATC 2 cut(s) 541, 641
DpnII GATC 2 cut(s) 539, 639
EciI GGCGGA 1 cut(s) 456
Eco130I CCWWGG 2 cut(s) 258, 769
Eco147I AGGCCT 2 cut(s) 14, 63
Eco47I GGWCC 1 cut(s) 443
Eco72I CACGTG 1 cut(s) 452
EcoRI GAATTC 2 cut(s) 168, 774
EcoRII CCWGG 2 cut(s) 135, 510
EcoT14I CCWWGG 2 cut(s) 258, 769
ErhI CCWWGG 2 cut(s) 258, 769
FaeI CATG 5 cut(s) 157, 228, 262, 683, 773
FalI AAGNNNNNCTT 2 cut(s) 51, 83
FaqI GGGAC 1 cut(s) 337
FatI CATG 5 cut(s) 153, 224, 258, 679, 769
FauI CCCGC 1 cut(s) 123
FblI GTMKAC 1 cut(s) 209
Fnu4HI GCNGC 3 cut(s) 74, 230, 522
Fsp4HI GCNGC 3 cut(s) 74, 230, 522
FspBI CTAG 1 cut(s) 126
GluI GCNGC 3 cut(s) 74, 230, 522
GsuI CTGGAG 1 cut(s) 573
HaeIII GGCC 3 cut(s) 14, 63, 601
HapII CCGG 1 cut(s) 103
Hin1II CATG 5 cut(s) 157, 228, 262, 683, 773
HincII GTYRAC 1 cut(s) 133
HindII GTYRAC 1 cut(s) 133
HinfI GANTC 3 cut(s) 178, 254, 565
HpaII CCGG 1 cut(s) 103
HphI GGTGA 1 cut(s) 576
Hpy166II GTNNAC 2 cut(s) 133, 210
Hpy188I TCNGA 1 cut(s) 177
Hpy188III TCNNGA 3 cut(s) 165, 485, 680
Hpy8I GTNNAC 2 cut(s) 133, 210
HpyAV CCTTC 2 cut(s) 53, 74
HpyCH4III ACNGT 6 cut(s) 207, 281, 428, 532, 628, 647
HpyCH4IV ACGT 2 cut(s) 451, 581
HpyCH4V TGCA 5 cut(s) 153, 157, 190, 380, 686
HpyF10VI GCNNNNNNNGC 2 cut(s) 159, 521
HpyF3I CTNAG 3 cut(s) 163, 327, 462
HpySE526I ACGT 2 cut(s) 451, 581
Hsp92II CATG 5 cut(s) 157, 228, 262, 683, 773
Kzo9I GATC 2 cut(s) 539, 639
LmnI GCTCC 3 cut(s) 105, 397, 529
Lsp1109I GCAGC 3 cut(s) 60, 216, 508
LweI GCATC 2 cut(s) 423, 673
MaeI CTAG 1 cut(s) 126
MaeII ACGT 2 cut(s) 451, 581
MaeIII GTNAC 2 cut(s) 582, 628
MalI GATC 2 cut(s) 541, 641
MbiI CCGCTC 1 cut(s) 712
MboI GATC 2 cut(s) 539, 639
MboII GAAGA 2 cut(s) 385, 760
MflI RGATCY 1 cut(s) 539
MhlI GDGCHC 1 cut(s) 164
MluCI AATT 6 cut(s) 168, 296, 307, 332, 753, 774
MlyI GAGTC 1 cut(s) 187
MmeI TCCRAC 1 cut(s) 447
MnlI CCTC 8 cut(s) 25, 41, 56, 346, 481, 484, 597, 717
MroXI GAANNNNTTC 1 cut(s) 63
MseI TTAA 2 cut(s) 69, 758
MspCI CTTAAG 1 cut(s) 68
MspI CCGG 1 cut(s) 103
MspR9I CCNGG 2 cut(s) 137, 512
Mva1269I GAATGC 1 cut(s) 382
MvaI CCWGG 2 cut(s) 137, 512
MvnI CGCG 1 cut(s) 118
MwoI GCNNNNNNNGC 2 cut(s) 159, 521
NcoI CCATGG 2 cut(s) 258, 769
NdeII GATC 2 cut(s) 539, 639
NlaIII CATG 5 cut(s) 157, 228, 262, 683, 773
NlaIV GGNNCC 2 cut(s) 445, 600
NmuCI GTSAC 2 cut(s) 582, 628
NspI RCATGY 1 cut(s) 157
NspV TTCGAA 1 cut(s) 371
PaeI GCATGC 1 cut(s) 157
PagI TCATGA 1 cut(s) 679
PceI AGGCCT 2 cut(s) 14, 63
PctI GAATGC 1 cut(s) 382
PdmI GAANNNNTTC 1 cut(s) 63
PfeI GAWTC 2 cut(s) 254, 565
PkrI GCNGC 3 cut(s) 75, 231, 523
PleI GAGTC 1 cut(s) 186
PmaCI CACGTG 1 cut(s) 452
PmlI CACGTG 1 cut(s) 452
PpsI GAGTC 1 cut(s) 186
Ppu21I YACGTR 1 cut(s) 452
Psp6I CCWGG 2 cut(s) 135, 510
PspCI CACGTG 1 cut(s) 452
PspGI CCWGG 2 cut(s) 135, 510
PspN4I GGNNCC 2 cut(s) 445, 600
PspPI GGNCC 2 cut(s) 443, 599
PstNI CAGNNNCTG 1 cut(s) 521
PsuI RGATCY 1 cut(s) 539
RsaI GTAC 3 cut(s) 204, 580, 649
RsaNI GTAC 3 cut(s) 203, 579, 648
SaqAI TTAA 2 cut(s) 69, 758
SatI GCNGC 3 cut(s) 74, 230, 522
Sau3AI GATC 2 cut(s) 539, 639
Sau96I GGNCC 2 cut(s) 443, 599
SchI GAGTC 1 cut(s) 187
ScrFI CCNGG 2 cut(s) 137, 512
SduI GDGCHC 1 cut(s) 164
SfaNI GCATC 2 cut(s) 423, 673
SfcI CTRYAG 2 cut(s) 528, 741
SfuI TTCGAA 1 cut(s) 371
SinI GGWCC 1 cut(s) 443
SmlI CTYRAG 3 cut(s) 47, 68, 485
SmoI CTYRAG 3 cut(s) 47, 68, 485
SphI GCATGC 1 cut(s) 157
Sse9I AATT 6 cut(s) 168, 296, 307, 332, 753, 774
SseBI AGGCCT 2 cut(s) 14, 63
SsiI CCGC 3 cut(s) 116, 441, 710
SspI AATATT 1 cut(s) 144
SspMI CTAG 1 cut(s) 126
StuI AGGCCT 2 cut(s) 14, 63
StyD4I CCNGG 2 cut(s) 135, 510
StyI CCWWGG 2 cut(s) 258, 769
TaaI ACNGT 6 cut(s) 207, 281, 428, 532, 628, 647
TaiI ACGT 2 cut(s) 454, 584
TaqI TCGA 1 cut(s) 371
TasI AATT 6 cut(s) 168, 296, 307, 332, 753, 774
TfiI GAWTC 2 cut(s) 254, 565
Tru1I TTAA 2 cut(s) 69, 758
Tru9I TTAA 2 cut(s) 69, 758
TscAI CASTG 2 cut(s) 164, 537
TseFI GTSAC 2 cut(s) 582, 628
TseI GCWGC 3 cut(s) 73, 229, 521
Tsp45I GTSAC 2 cut(s) 582, 628
TspDTI ATGAA 4 cut(s) 17, 161, 282, 668
TspGWI ACGGA 1 cut(s) 7
TspRI CASTG 2 cut(s) 164, 537
Vha464I CTTAAG 1 cut(s) 68
VpaK11BI GGWCC 1 cut(s) 443
XapI RAATTY 4 cut(s) 168, 307, 332, 774
XceI RCATGY 1 cut(s) 157
XmiI GTMKAC 1 cut(s) 209
XmnI GAANNNNTTC 1 cut(s) 63
XspI CTAG 1 cut(s) 126
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.