MD05G1272000.v1.1

Protein SCAI

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0010387
Physical Location & Seq
Forward (+)
40720468 .. 40726154
5687 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1272000.v1.1.491

Sequence Viewer

Length: 456 bp
ATGGATCATGTTCTCATTTGTAGATCGATTGGTAGGCCTTTGTATCCAACTATTTTTAGCGGCTTCTACCATGAAACTCAGATACTCTCTTGGTTTCTTCCTTATGCACACGAGGTAATTCCACGAGGTTATTCTGAGCAGAGAGTACTCAAGGAGGGCATGTTTGAGGACCTGAGCTTGGCCAACAAGCAGCTCCGGTTTGTTTTGAGATTTTTAATGGTGTGCTTTCTCTTGAATCGGCGAGAGATGGTCTGGCAGCTGCTCAATCAGCTCAGAGTCTTGGTTGATGAGTGCAAGAGGACCTTCCAGCCATGGGTAATGAAAAGGGAAACAGTTATTGGAAAAAAGAATTGCCACCAAATTTTGACAGAAGTGGGACAGAGGAATTTATTCGTGCCAAATATGAGGGAAAACTGTGGGTTGCGAAGAATGCAAAGCAACCAGTTCCTCTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SCAI

Protein Analysis

152

Amino Acids

18.11

Weight (kDa)

9.61

Isoelectric Point (pI)

51.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SCAI PF12070 39 - 104 2.8e-13 Protein SCAI
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 60
AclWI GGATC 1 cut(s) 12
AcoI YGGCCR 1 cut(s) 180
AcsI RAATTY 2 cut(s) 360, 385
AfaI GTAC 1 cut(s) 147
AfiI CCNNNNNNNGG 2 cut(s) 178, 313
AgsI TTSAA 1 cut(s) 235
AjuI GAANNNNNNNTTGG 2 cut(s) 321, 353
AluBI AGCT 4 cut(s) 177, 193, 259, 271
AluI AGCT 4 cut(s) 177, 193, 259, 271
AlwI GGATC 1 cut(s) 12
AoxI GGCC 2 cut(s) 35, 180
ApeKI GCWGC 3 cut(s) 190, 256, 259
ApoI RAATTY 2 cut(s) 360, 385
Asp700I GAANNNNTTC 1 cut(s) 389
AspS9I GGNCC 2 cut(s) 169, 300
AvaII GGWCC 2 cut(s) 169, 300
BalI TGGCCA 1 cut(s) 182
BauI CACGAG 2 cut(s) 110, 123
BbvI GCAGC 3 cut(s) 202, 246, 268
BccI CCATC 1 cut(s) 241
BciVI GTATCC 1 cut(s) 54
BfuI GTATCC 1 cut(s) 54
BisI GCNGC 4 cut(s) 61, 191, 257, 260
BlsI GCNGC 4 cut(s) 62, 192, 258, 261
BmcAI AGTACT 1 cut(s) 147
Bme18I GGWCC 2 cut(s) 169, 300
BmgT120I GGNCC 2 cut(s) 169, 300
Bpu10I CCTNAGC 1 cut(s) 173
BpuEI CTTGAG 1 cut(s) 134
Bsa29I ATCGAT 1 cut(s) 26
BsaJI CCNNGG 1 cut(s) 311
BsaWI WCCGGW 1 cut(s) 195
BsaXI ACNNNNNCTCC 2 cut(s) 146, 176
Bsc4I CCNNNNNNNGG 2 cut(s) 178, 313
Bse1I ACTGG 1 cut(s) 442
BseCI ATCGAT 1 cut(s) 26
BseDI CCNNGG 1 cut(s) 311
BseLI CCNNNNNNNGG 2 cut(s) 178, 313
BseMII CTCAG 4 cut(s) 92, 126, 164, 286
BseNI ACTGG 1 cut(s) 442
BseXI GCAGC 3 cut(s) 202, 246, 268
BshFI GGCC 2 cut(s) 37, 182
BshVI ATCGAT 1 cut(s) 26
BsiSI CCGG 1 cut(s) 196
BslFI GGGAC 1 cut(s) 390
BslI CCNNNNNNNGG 2 cut(s) 178, 313
BsmFI GGGAC 1 cut(s) 390
BsmI GAATGC 1 cut(s) 435
BsnI GGCC 2 cut(s) 37, 182
Bsp143I GATC 2 cut(s) 4, 23
Bsp19I CCATGG 1 cut(s) 311
BspACI CCGC 1 cut(s) 60
BspANI GGCC 2 cut(s) 37, 182
BspCNI CTCAG 4 cut(s) 91, 127, 165, 285
BspDI ATCGAT 1 cut(s) 26
BspPI GGATC 1 cut(s) 12
BsrI ACTGG 1 cut(s) 442
BssECI CCNNGG 1 cut(s) 311
BssMI GATC 2 cut(s) 4, 23
BssSI CACGAG 2 cut(s) 110, 123
BssT1I CCWWGG 1 cut(s) 311
Bst2BI CACGAG 2 cut(s) 110, 123
Bst4CI ACNGT 2 cut(s) 334, 416
BstDEI CTNAG 4 cut(s) 78, 135, 173, 272
BstDSI CCRYGG 1 cut(s) 311
BstKTI GATC 2 cut(s) 7, 26
BstMBI GATC 2 cut(s) 4, 23
BstMWI GCNNNNNNNGC 2 cut(s) 268, 430
BstNSI RCATGY 1 cut(s) 163
BstV1I GCAGC 3 cut(s) 202, 246, 268
Bsu15I ATCGAT 1 cut(s) 26
BsuI GTATCC 1 cut(s) 54
BsuRI GGCC 2 cut(s) 37, 182
BsuTUI ATCGAT 1 cut(s) 26
BtgI CCRYGG 1 cut(s) 311
Cfr13I GGNCC 2 cut(s) 169, 300
ClaI ATCGAT 1 cut(s) 26
Csp6I GTAC 1 cut(s) 146
CviAII CATG 4 cut(s) 8, 71, 160, 312
CviJI RGCY 8 cut(s) 37, 63, 177, 182, 193, 259, 271, 310
CviKI_1 RGCY 8 cut(s) 37, 63, 177, 182, 193, 259, 271, 310
CviQI GTAC 1 cut(s) 146
DdeI CTNAG 4 cut(s) 78, 135, 173, 272
DpnI GATC 2 cut(s) 6, 25
DpnII GATC 2 cut(s) 4, 23
EaeI YGGCCR 1 cut(s) 180
Eco130I CCWWGG 1 cut(s) 311
Eco147I AGGCCT 1 cut(s) 37
Eco47I GGWCC 2 cut(s) 169, 300
EcoO109I RGGNCCY 2 cut(s) 169, 300
EcoT14I CCWWGG 1 cut(s) 311
ErhI CCWWGG 1 cut(s) 311
FaeI CATG 4 cut(s) 11, 74, 163, 315
FaiI YATR 6 cut(s) 9, 72, 105, 161, 313, 404
FalI AAGNNNNNCTT 2 cut(s) 287, 319
FaqI GGGAC 1 cut(s) 390
FatI CATG 4 cut(s) 7, 70, 159, 311
Fnu4HI GCNGC 4 cut(s) 61, 191, 257, 260
Fsp4HI GCNGC 4 cut(s) 61, 191, 257, 260
GluI GCNGC 4 cut(s) 61, 191, 257, 260
HaeIII GGCC 2 cut(s) 37, 182
HapII CCGG 1 cut(s) 196
Hin1II CATG 4 cut(s) 11, 74, 163, 315
HinfI GANTC 2 cut(s) 235, 276
HpaII CCGG 1 cut(s) 196
Hpy188I TCNGA 3 cut(s) 81, 136, 275
Hpy188III TCNNGA 1 cut(s) 232
HpyAV CCTTC 1 cut(s) 313
HpyCH4III ACNGT 2 cut(s) 334, 416
HpyCH4V TGCA 3 cut(s) 107, 294, 433
HpyF10VI GCNNNNNNNGC 2 cut(s) 268, 430
HpyF3I CTNAG 4 cut(s) 78, 135, 173, 272
Hsp92II CATG 4 cut(s) 11, 74, 163, 315
Kzo9I GATC 2 cut(s) 4, 23
LmnI GCTCC 1 cut(s) 198
LpnPI CCDG 4 cut(s) 185, 209, 238, 320
Lsp1109I GCAGC 3 cut(s) 202, 246, 268
MalI GATC 2 cut(s) 6, 25
MboI GATC 2 cut(s) 4, 23
MboII GAAGA 2 cut(s) 89, 438
MlsI TGGCCA 1 cut(s) 182
MluCI AATT 4 cut(s) 117, 349, 360, 385
MluNI TGGCCA 1 cut(s) 182
MlyI GAGTC 1 cut(s) 285
MmeI TCCRAC 1 cut(s) 71
MnlI CCTC 7 cut(s) 106, 119, 148, 160, 291, 375, 399
Mox20I TGGCCA 1 cut(s) 182
MroXI GAANNNNTTC 1 cut(s) 389
MscI TGGCCA 1 cut(s) 182
MseI TTAA 2 cut(s) 215, 454
Msp20I TGGCCA 1 cut(s) 182
MspA1I CMGCKG 1 cut(s) 259
MspI CCGG 1 cut(s) 196
Mva1269I GAATGC 1 cut(s) 435
MwoI GCNNNNNNNGC 2 cut(s) 268, 430
NcoI CCATGG 1 cut(s) 311
NdeII GATC 2 cut(s) 4, 23
NlaIII CATG 4 cut(s) 11, 74, 163, 315
NspI RCATGY 1 cut(s) 163
PceI AGGCCT 1 cut(s) 37
PctI GAATGC 1 cut(s) 435
PdmI GAANNNNTTC 1 cut(s) 389
PfeI GAWTC 1 cut(s) 235
PkrI GCNGC 4 cut(s) 62, 192, 258, 261
PleI GAGTC 1 cut(s) 284
PpsI GAGTC 1 cut(s) 284
PpuMI RGGWCCY 2 cut(s) 169, 300
Psp5II RGGWCCY 2 cut(s) 169, 300
PspPI GGNCC 2 cut(s) 169, 300
PspPPI RGGWCCY 2 cut(s) 169, 300
PvuII CAGCTG 1 cut(s) 259
RsaI GTAC 1 cut(s) 147
RsaNI GTAC 1 cut(s) 146
SaqAI TTAA 2 cut(s) 215, 454
SatI GCNGC 4 cut(s) 61, 191, 257, 260
Sau3AI GATC 2 cut(s) 4, 23
Sau96I GGNCC 2 cut(s) 169, 300
ScaI AGTACT 1 cut(s) 147
SchI GAGTC 1 cut(s) 285
SetI ASST 8 cut(s) 117, 130, 174, 179, 195, 261, 273, 305
SinI GGWCC 2 cut(s) 169, 300
SmlI CTYRAG 1 cut(s) 149
SmoI CTYRAG 1 cut(s) 149
Sse9I AATT 4 cut(s) 117, 349, 360, 385
SseBI AGGCCT 1 cut(s) 37
SsiI CCGC 1 cut(s) 60
StuI AGGCCT 1 cut(s) 37
StyI CCWWGG 1 cut(s) 311
TaaI ACNGT 2 cut(s) 334, 416
TaqI TCGA 1 cut(s) 26
TasI AATT 4 cut(s) 117, 349, 360, 385
TatI WGTACW 1 cut(s) 145
TauI GCSGC 1 cut(s) 63
TfiI GAWTC 1 cut(s) 235
Tru1I TTAA 2 cut(s) 215, 454
Tru9I TTAA 2 cut(s) 215, 454
TseI GCWGC 3 cut(s) 190, 256, 259
TspDTI ATGAA 2 cut(s) 87, 335
VpaK11BI GGWCC 2 cut(s) 169, 300
XapI RAATTY 2 cut(s) 360, 385
XceI RCATGY 1 cut(s) 163
XmnI GAANNNNTTC 1 cut(s) 389
ZrmI AGTACT 1 cut(s) 147
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.