MD12G1051500.v1.1

ADP-ribosylation factor GTPase-activating protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0010387
Physical Location & Seq
Reverse (-)
5846672 .. 5851558
4887 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1051500.v1.1.491

Sequence Viewer

Length: 453 bp
ATGTTTCAGGACCTGAGCTGGGGGAACAAGAAGCATCGGTTTGTTTCGAGATTTTTAATGGTGTGCTTGCTCTTGAATTGGCTAGAGATGGTGCAGCAGCTGCTCAATCAGCTCAGAGTCGTGGTTGATAAGCGCAAGAGGACCTTCCAGGTTTTGAATGAAAAGGGAAATAGTTATTGGGAAAAGAATTGCCACCAAATTTTGGGACTGAGAAAGCTATTCGTGCCAAGTATGAGGGAAGACTGGGTTGCGAAGAATGCAAAACAACCAGTTCCTAAACCAGTAGATATGAGCATCAGTTCCAATAGTTTGGTAAAGGATGGAGCCACATGTGGAGCTCCAAAGGAAACAAGGAGACATTCTCTTGAGGAAGCGATGCTTTTTACGGTGGCGGAAGCTGCTCCTCCAGTGGCAAGATCCCGTGGGTTACTTTTCAGCATCCGACAACACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
ArfGap

Protein Analysis

151

Amino Acids

17.35

Weight (kDa)

10.08

Isoelectric Point (pI)

49.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SCAI PF12070 3 - 56 5.6e-11 Protein SCAI
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 202
AciI CCGC 1 cut(s) 392
AclWI GGATC 1 cut(s) 411
AcsI RAATTY 1 cut(s) 198
AfiI CCNNNNNNNGG 2 cut(s) 19, 202
AflIII ACRYGT 1 cut(s) 329
AgsI TTSAA 2 cut(s) 76, 157
AjnI CCWGG 1 cut(s) 147
AjuI GAANNNNNNNTTGG 2 cut(s) 160, 192
AluBI AGCT 6 cut(s) 18, 100, 112, 217, 338, 398
AluI AGCT 6 cut(s) 18, 100, 112, 217, 338, 398
Alw21I GWGCWC 1 cut(s) 340
Alw26I GTCTC 1 cut(s) 349
AlwI GGATC 1 cut(s) 411
AlwNI CAGNNNCTG 2 cut(s) 13, 100
ApeKI GCWGC 4 cut(s) 94, 97, 100, 398
ApoI RAATTY 1 cut(s) 198
AspLEI GCGC 1 cut(s) 135
AspS9I GGNCC 2 cut(s) 10, 141
AvaII GGWCC 2 cut(s) 10, 141
BanII GRGCYC 1 cut(s) 340
BbsI GAAGAC 1 cut(s) 246
Bbv12I GWGCWC 1 cut(s) 340
BbvI GCAGC 4 cut(s) 87, 106, 109, 385
BccI CCATC 2 cut(s) 82, 314
BciT130I CCWGG 1 cut(s) 149
BcoDI GTCTC 1 cut(s) 349
BfaI CTAG 1 cut(s) 83
BisI GCNGC 4 cut(s) 95, 98, 101, 399
BlsI GCNGC 4 cut(s) 96, 99, 102, 400
Bme1390I CCNGG 1 cut(s) 149
Bme18I GGWCC 2 cut(s) 10, 141
BmgT120I GGNCC 2 cut(s) 10, 141
BmiI GGNNCC 1 cut(s) 325
BmrFI CCNGG 1 cut(s) 149
BmrI ACTGGG 1 cut(s) 253
BmsI GCATC 4 cut(s) 43, 303, 366, 447
BmuI ACTGGG 1 cut(s) 253
BpiI GAAGAC 1 cut(s) 246
BplI GAGNNNNNCTC 2 cut(s) 346, 378
BpmI CTGGAG 1 cut(s) 390
Bpu10I CCTNAGC 1 cut(s) 14
BpuEI CTTGAG 1 cut(s) 386
BsaJI CCNNGG 1 cut(s) 421
Bsc4I CCNNNNNNNGG 2 cut(s) 19, 202
Bse1I ACTGG 4 cut(s) 248, 269, 281, 407
BseBI CCWGG 1 cut(s) 149
BseDI CCNNGG 1 cut(s) 421
BseGI GGATG 2 cut(s) 325, 438
BseLI CCNNNNNNNGG 2 cut(s) 19, 202
BseMII CTCAG 3 cut(s) 5, 127, 200
BseNI ACTGG 4 cut(s) 248, 269, 281, 407
BseRI GAGGAG 1 cut(s) 393
BseXI GCAGC 4 cut(s) 87, 106, 109, 385
BseYI CCCAGC 1 cut(s) 18
BsgI GTGCAG 1 cut(s) 113
BsiHKAI GWGCWC 1 cut(s) 340
BslFI GGGAC 1 cut(s) 219
BslI CCNNNNNNNGG 2 cut(s) 19, 202
BsmAI GTCTC 1 cut(s) 349
BsmFI GGGAC 1 cut(s) 219
BsmI GAATGC 1 cut(s) 262
Bsp1286I GDGCHC 1 cut(s) 340
Bsp143I GATC 1 cut(s) 416
BspACI CCGC 1 cut(s) 392
BspCNI CTCAG 3 cut(s) 6, 126, 201
BspLI GGNNCC 1 cut(s) 325
BspPI GGATC 1 cut(s) 411
BsrI ACTGG 4 cut(s) 248, 269, 281, 407
BssECI CCNNGG 1 cut(s) 421
BssMI GATC 1 cut(s) 416
Bst2UI CCWGG 1 cut(s) 149
Bst4CI ACNGT 1 cut(s) 388
BstAPI GCANNNNNTGC 1 cut(s) 100
BstC8I GCNNGC 1 cut(s) 68
BstDEI CTNAG 3 cut(s) 14, 113, 209
BstDSI CCRYGG 1 cut(s) 421
BstF5I GGATG 2 cut(s) 325, 438
BstHHI GCGC 1 cut(s) 135
BstKTI GATC 1 cut(s) 419
BstMAI GTCTC 1 cut(s) 349
BstMBI GATC 1 cut(s) 416
BstMWI GCNNNNNNNGC 5 cut(s) 100, 109, 223, 257, 398
BstNI CCWGG 1 cut(s) 149
BstNSI RCATGY 1 cut(s) 333
BstSCI CCNGG 1 cut(s) 147
BstV1I GCAGC 4 cut(s) 87, 106, 109, 385
BstV2I GAAGAC 1 cut(s) 246
BstX2I RGATCY 1 cut(s) 416
BstXI CCANNNNNNTGG 1 cut(s) 310
BstYI RGATCY 1 cut(s) 416
BtgI CCRYGG 1 cut(s) 421
BtgZI GCGATG 1 cut(s) 389
BtsCI GGATG 2 cut(s) 325, 438
BtsIMutI CAGTG 2 cut(s) 414, 448
Cac8I GCNNGC 1 cut(s) 68
CaiI CAGNNNCTG 2 cut(s) 13, 100
CfoI GCGC 1 cut(s) 135
Cfr13I GGNCC 2 cut(s) 10, 141
CviAII CATG 1 cut(s) 330
CviJI RGCY 8 cut(s) 18, 82, 100, 112, 217, 326, 338, 398
CviKI_1 RGCY 8 cut(s) 18, 82, 100, 112, 217, 326, 338, 398
DdeI CTNAG 3 cut(s) 14, 113, 209
DpnI GATC 1 cut(s) 418
DpnII GATC 1 cut(s) 416
EciI GGCGGA 1 cut(s) 407
Ecl136II GAGCTC 1 cut(s) 338
Eco24I GRGCYC 1 cut(s) 340
Eco47I GGWCC 2 cut(s) 10, 141
Eco53kI GAGCTC 1 cut(s) 338
EcoICRI GAGCTC 1 cut(s) 338
EcoO109I RGGNCCY 2 cut(s) 10, 141
EcoRII CCWGG 1 cut(s) 147
EcoT38I GRGCYC 1 cut(s) 340
FaeI CATG 1 cut(s) 333
FaiI YATR 3 cut(s) 233, 290, 331
FalI AAGNNNNNCTT 4 cut(s) 128, 160, 363, 395
FaqI GGGAC 1 cut(s) 219
FatI CATG 1 cut(s) 329
Fnu4HI GCNGC 4 cut(s) 95, 98, 101, 399
FokI GGATG 2 cut(s) 332, 425
FriOI GRGCYC 1 cut(s) 340
Fsp4HI GCNGC 4 cut(s) 95, 98, 101, 399
FspBI CTAG 1 cut(s) 83
GlaI GCGC 1 cut(s) 134
GluI GCNGC 4 cut(s) 95, 98, 101, 399
GsaI CCCAGC 1 cut(s) 22
GsuI CTGGAG 1 cut(s) 390
HhaI GCGC 1 cut(s) 135
Hin1II CATG 1 cut(s) 333
Hin6I GCGC 1 cut(s) 133
HinP1I GCGC 1 cut(s) 133
HinfI GANTC 1 cut(s) 117
Hpy188I TCNGA 2 cut(s) 116, 443
Hpy188III TCNNGA 4 cut(s) 8, 48, 73, 365
HpyAV CCTTC 1 cut(s) 154
HpyCH4III ACNGT 1 cut(s) 388
HpyCH4V TGCA 2 cut(s) 94, 260
HpyF10VI GCNNNNNNNGC 5 cut(s) 100, 109, 223, 257, 398
HpyF3I CTNAG 3 cut(s) 14, 113, 209
Hsp92II CATG 1 cut(s) 333
HspAI GCGC 1 cut(s) 133
Kzo9I GATC 1 cut(s) 416
LmnI GCTCC 4 cut(s) 323, 335, 343, 406
LpnPI CCDG 8 cut(s) 4, 26, 134, 161, 229, 282, 294, 420
Lsp1109I GCAGC 4 cut(s) 87, 106, 109, 385
LweI GCATC 4 cut(s) 43, 303, 366, 447
MaeI CTAG 1 cut(s) 83
MaeIII GTNAC 1 cut(s) 426
MalI GATC 1 cut(s) 418
MboI GATC 1 cut(s) 416
MboII GAAGA 2 cut(s) 251, 265
MflI RGATCY 1 cut(s) 416
MhlI GDGCHC 1 cut(s) 340
MluCI AATT 3 cut(s) 76, 187, 198
MlyI GAGTC 1 cut(s) 126
MnlI CCTC 4 cut(s) 132, 228, 361, 414
MseI TTAA 1 cut(s) 56
MspA1I CMGCKG 1 cut(s) 100
MspR9I CCNGG 1 cut(s) 149
Mva1269I GAATGC 1 cut(s) 262
MvaI CCWGG 1 cut(s) 149
MwoI GCNNNNNNNGC 5 cut(s) 100, 109, 223, 257, 398
NdeII GATC 1 cut(s) 416
NlaIII CATG 1 cut(s) 333
NlaIV GGNNCC 1 cut(s) 325
NspI RCATGY 1 cut(s) 333
PciI ACATGT 1 cut(s) 329
PctI GAATGC 1 cut(s) 262
PflMI CCANNNNNTGG 1 cut(s) 202
PkrI GCNGC 4 cut(s) 96, 99, 102, 400
PleI GAGTC 1 cut(s) 125
PpsI GAGTC 1 cut(s) 125
PpuMI RGGWCCY 2 cut(s) 10, 141
PscI ACATGT 1 cut(s) 329
Psp124BI GAGCTC 1 cut(s) 340
Psp5II RGGWCCY 2 cut(s) 10, 141
Psp6I CCWGG 1 cut(s) 147
PspFI CCCAGC 1 cut(s) 18
PspGI CCWGG 1 cut(s) 147
PspN4I GGNNCC 1 cut(s) 325
PspPI GGNCC 2 cut(s) 10, 141
PspPPI RGGWCCY 2 cut(s) 10, 141
PstNI CAGNNNCTG 2 cut(s) 13, 100
PsuI RGATCY 1 cut(s) 416
PvuII CAGCTG 1 cut(s) 100
SacI GAGCTC 1 cut(s) 340
SaqAI TTAA 1 cut(s) 56
SatI GCNGC 4 cut(s) 95, 98, 101, 399
Sau3AI GATC 1 cut(s) 416
Sau96I GGNCC 2 cut(s) 10, 141
SchI GAGTC 1 cut(s) 126
ScrFI CCNGG 1 cut(s) 149
SduI GDGCHC 1 cut(s) 340
SetI ASST 9 cut(s) 15, 20, 102, 114, 146, 153, 219, 340, 400
SfaNI GCATC 4 cut(s) 43, 303, 366, 447
SinI GGWCC 2 cut(s) 10, 141
SmlI CTYRAG 1 cut(s) 365
SmoI CTYRAG 1 cut(s) 365
Sse9I AATT 3 cut(s) 76, 187, 198
SsiI CCGC 1 cut(s) 392
SspMI CTAG 1 cut(s) 83
SstI GAGCTC 1 cut(s) 340
StyD4I CCNGG 1 cut(s) 147
TaaI ACNGT 1 cut(s) 388
TaqI TCGA 1 cut(s) 47
TasI AATT 3 cut(s) 76, 187, 198
Tru1I TTAA 1 cut(s) 56
Tru9I TTAA 1 cut(s) 56
TscAI CASTG 1 cut(s) 414
TseI GCWGC 4 cut(s) 94, 97, 100, 398
TspDTI ATGAA 1 cut(s) 174
TspRI CASTG 1 cut(s) 414
Van91I CCANNNNNTGG 1 cut(s) 202
VpaK11BI GGWCC 2 cut(s) 10, 141
XapI RAATTY 1 cut(s) 198
XceI RCATGY 1 cut(s) 333
XspI CTAG 1 cut(s) 83
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.