FvH4_5g18340

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0016850
Physical Location & Seq
Reverse (-)
10545049 .. 10546711
1663 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g18340.t1

Sequence Viewer

Length: 681 bp
ATGGATCAAAGCAAGGCAGCCGGAGCTGATCATCAGGCCATGGCCAAACGTACTTACGAGGACTTCGAACCCTTCTGCAAATGGCATAACCGCGACAATGTTGTTGAGGTCCATCTTCAAGGTTTCAGAAAGGAACAGCTGAGTGTCCGTACCACTCATACGGGGATGCTTACAATCCATGGAGAACGTCCATTGGACCAAACTAAATCTACATGGAGCCGCTTCCACAAAGAACTGAAGCTTCCAAACAACTGCGACACAAATAGAGTTGCTGCAAAATTTGCTGCAGGAGTTCTTACAATAACAATGCCTCCCAAAGTTGTTGCACAACCACCTCAATCTCCACATGAGGATGATCAGAAACAGCAGCTTGCTAATCGTGAAAAGTTACCGCGACCAAGCCACGTTGATAATCAAACAAATAATGATGAAGCTTTAGGTAAACTTATCAAACAAAATCAGGACGTAAACGCAACCAATGATGAAAATGCTGCATTGCTTGAGGAAGAGGTCAAGCAAGGAAGCAGCTGCCAATTCCGACAGAGAAGTAGTTTAGCCTCCAAAAAACACATTGCAGTGAAACTTGTGTTGGTGGTTGCCGCGGTGGTCGCTCTGGGTTTCGGGGCATATGTCCTATACAAACACGGCCATCACTCATATAGCTCGCTGCAAGTAGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HSP20

Protein Analysis

227

Amino Acids

25.37

Weight (kDa)

8.7

Isoelectric Point (pI)

42.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 34 - 111 3.4e-12 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016850)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g18340
malus_domestica MD06G1239100.v1.1
prunus_persica Prupe.5G242900_v2.0.a1
pyrus_communis pycom06g21460
rosa_chinensis RchiOBHm_Chr7g0177251
rosa_laevigata RLG00000005551
rosa_multiflora Rmu_sc0005310.1_g000013
rosa_roxburghii Rroxscaffold_3G00276010
rosa_rugosa Rorug06G0404200
rosa_samantha Rh7AG004200 Rh7BG004000 Rh7CG003900 Rh7DG003900
rosa_wichuraiana Rw7G000340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 675
AccII CGCG 3 cut(s) 93, 394, 602
AciI CCGC 5 cut(s) 91, 220, 392, 600, 602
AclWI GGATC 1 cut(s) 12
AcoI YGGCCR 2 cut(s) 42, 646
AcsI RAATTY 1 cut(s) 278
AcuI CTGAAG 1 cut(s) 257
AfaI GTAC 2 cut(s) 52, 151
AgsI TTSAA 1 cut(s) 119
AjuI GAANNNNNNNTTGG 2 cut(s) 572, 604
AluBI AGCT 7 cut(s) 26, 139, 241, 370, 434, 528, 663
AluI AGCT 7 cut(s) 26, 139, 241, 370, 434, 528, 663
AlwI GGATC 1 cut(s) 12
AoxI GGCC 3 cut(s) 36, 42, 646
ApeKI GCWGC 8 cut(s) 17, 272, 284, 367, 491, 525, 528, 667
ApoI RAATTY 1 cut(s) 278
AspS9I GGNCC 2 cut(s) 109, 196
AsuII TTCGAA 1 cut(s) 66
AvaII GGWCC 2 cut(s) 109, 196
BalI TGGCCA 1 cut(s) 44
BbvI GCAGC 8 cut(s) 29, 259, 271, 379, 478, 515, 537, 654
BccI CCATC 2 cut(s) 120, 657
BceAI ACGGC 1 cut(s) 661
BclI TGATCA 2 cut(s) 28, 355
BfaI CTAG 1 cut(s) 679
BfmI CTRYAG 1 cut(s) 285
Bme18I GGWCC 2 cut(s) 109, 196
BmgT120I GGNCC 2 cut(s) 109, 196
BmiI GGNNCC 1 cut(s) 218
BmsI GCATC 1 cut(s) 156
Bpu14I TTCGAA 1 cut(s) 66
BpuEI CTTGAG 1 cut(s) 521
BsaJI CCNNGG 3 cut(s) 39, 178, 600
BsaXI ACNNNNNCTCC 2 cut(s) 295, 325
Bse3DI GCAATG 2 cut(s) 494, 570
BseDI CCNNGG 3 cut(s) 39, 178, 600
BseGI GGATG 2 cut(s) 171, 358
BseMI GCAATG 2 cut(s) 494, 570
BseMII CTCAG 1 cut(s) 131
BseXI GCAGC 8 cut(s) 29, 259, 271, 379, 478, 515, 537, 654
Bsh1236I CGCG 3 cut(s) 93, 394, 602
BshFI GGCC 3 cut(s) 38, 44, 648
BsiSI CCGG 1 cut(s) 21
BsnI GGCC 3 cut(s) 38, 44, 648
Bsp119I TTCGAA 1 cut(s) 66
Bsp143I GATC 3 cut(s) 4, 28, 355
Bsp19I CCATGG 2 cut(s) 39, 178
BspACI CCGC 5 cut(s) 91, 220, 392, 600, 602
BspANI GGCC 3 cut(s) 38, 44, 648
BspCNI CTCAG 1 cut(s) 132
BspFNI CGCG 3 cut(s) 93, 394, 602
BspLI GGNNCC 1 cut(s) 218
BspMAI CTGCAG 1 cut(s) 289
BspPI GGATC 1 cut(s) 12
BspT104I TTCGAA 1 cut(s) 66
BsrDI GCAATG 2 cut(s) 494, 570
BssECI CCNNGG 3 cut(s) 39, 178, 600
BssMI GATC 3 cut(s) 4, 28, 355
BssT1I CCWWGG 2 cut(s) 39, 178
Bst6I CTCTTC 1 cut(s) 501
BstAPI GCANNNNNTGC 1 cut(s) 281
BstBI TTCGAA 1 cut(s) 66
BstC8I GCNNGC 2 cut(s) 372, 665
BstDEI CTNAG 1 cut(s) 140
BstDSI CCRYGG 3 cut(s) 39, 178, 600
BstF5I GGATG 2 cut(s) 171, 358
BstFNI CGCG 3 cut(s) 93, 394, 602
BstKTI GATC 3 cut(s) 7, 31, 358
BstMBI GATC 3 cut(s) 4, 28, 355
BstMWI GCNNNNNNNGC 3 cut(s) 23, 281, 608
BstSFI CTRYAG 1 cut(s) 285
BstUI CGCG 3 cut(s) 93, 394, 602
BstV1I GCAGC 8 cut(s) 29, 259, 271, 379, 478, 515, 537, 654
BsuRI GGCC 3 cut(s) 38, 44, 648
BtgI CCRYGG 3 cut(s) 39, 178, 600
BtsCI GGATG 2 cut(s) 171, 358
BtsI GCAGTG 1 cut(s) 582
BtsIMutI CAGTG 1 cut(s) 582
Cac8I GCNNGC 2 cut(s) 372, 665
Cfr13I GGNCC 2 cut(s) 109, 196
Cfr42I CCGCGG 1 cut(s) 603
Csp6I GTAC 2 cut(s) 51, 150
CviAII CATG 4 cut(s) 40, 179, 213, 347
CviQI GTAC 2 cut(s) 51, 150
DdeI CTNAG 1 cut(s) 140
DpnI GATC 3 cut(s) 6, 30, 357
DpnII GATC 3 cut(s) 4, 28, 355
EaeI YGGCCR 2 cut(s) 42, 646
Eam1104I CTCTTC 1 cut(s) 501
EarI CTCTTC 1 cut(s) 501
Eco130I CCWWGG 2 cut(s) 39, 178
Eco47I GGWCC 2 cut(s) 109, 196
Eco57I CTGAAG 1 cut(s) 257
EcoT14I CCWWGG 2 cut(s) 39, 178
ErhI CCWWGG 2 cut(s) 39, 178
FaeI CATG 4 cut(s) 43, 182, 216, 350
FatI CATG 4 cut(s) 39, 178, 212, 346
FauNDI CATATG 1 cut(s) 628
FbaI TGATCA 2 cut(s) 28, 355
FblI GTMKAC 1 cut(s) 675
FokI GGATG 2 cut(s) 178, 365
FspBI CTAG 1 cut(s) 679
HaeIII GGCC 3 cut(s) 38, 44, 648
HapII CCGG 1 cut(s) 21
Hin1II CATG 4 cut(s) 43, 182, 216, 350
HindIII AAGCTT 2 cut(s) 239, 432
HpaII CCGG 1 cut(s) 21
Hpy166II GTNNAC 3 cut(s) 443, 469, 676
Hpy188I TCNGA 3 cut(s) 128, 360, 539
Hpy188III TCNNGA 2 cut(s) 380, 461
Hpy8I GTNNAC 3 cut(s) 443, 469, 676
HpyAV CCTTC 1 cut(s) 82
HpyCH4IV ACGT 4 cut(s) 49, 187, 405, 465
HpyCH4V TGCA 7 cut(s) 78, 275, 287, 326, 494, 575, 670
HpyF10VI GCNNNNNNNGC 3 cut(s) 23, 281, 608
HpyF3I CTNAG 1 cut(s) 140
HpySE526I ACGT 4 cut(s) 49, 187, 405, 465
Hsp92II CATG 4 cut(s) 43, 182, 216, 350
Ksp22I TGATCA 2 cut(s) 28, 355
KspI CCGCGG 1 cut(s) 603
Kzo9I GATC 3 cut(s) 4, 28, 355
LmnI GCTCC 2 cut(s) 23, 216
LpnPI CCDG 5 cut(s) 20, 34, 273, 446, 599
Lsp1109I GCAGC 8 cut(s) 29, 259, 271, 379, 478, 515, 537, 654
LweI GCATC 1 cut(s) 156
MaeI CTAG 1 cut(s) 679
MaeII ACGT 4 cut(s) 49, 187, 405, 465
MaeIII GTNAC 1 cut(s) 387
MalI GATC 3 cut(s) 6, 30, 357
MboI GATC 3 cut(s) 4, 28, 355
MboII GAAGA 2 cut(s) 107, 518
MlsI TGGCCA 1 cut(s) 44
MluCI AATT 2 cut(s) 278, 533
MluNI TGGCCA 1 cut(s) 44
MmeI TCCRAC 1 cut(s) 562
MnlI CCTC 8 cut(s) 52, 100, 321, 343, 345, 496, 502, 568
Mox20I TGGCCA 1 cut(s) 44
MscI TGGCCA 1 cut(s) 44
MslI CAYNNNNRTG 2 cut(s) 351, 575
Msp20I TGGCCA 1 cut(s) 44
MspA1I CMGCKG 3 cut(s) 139, 528, 602
MspI CCGG 1 cut(s) 21
MvnI CGCG 3 cut(s) 93, 394, 602
MwoI GCNNNNNNNGC 3 cut(s) 23, 281, 608
NcoI CCATGG 2 cut(s) 39, 178
NdeI CATATG 1 cut(s) 628
NdeII GATC 3 cut(s) 4, 28, 355
NlaIII CATG 4 cut(s) 43, 182, 216, 350
NlaIV GGNNCC 1 cut(s) 218
NspV TTCGAA 1 cut(s) 66
PcsI WCGNNNNNNNCGW 1 cut(s) 63
PspN4I GGNNCC 1 cut(s) 218
PspPI GGNCC 2 cut(s) 109, 196
PstI CTGCAG 1 cut(s) 289
PvuII CAGCTG 2 cut(s) 139, 528
RsaI GTAC 2 cut(s) 52, 151
RsaNI GTAC 2 cut(s) 51, 150
RseI CAYNNNNRTG 2 cut(s) 351, 575
SacII CCGCGG 1 cut(s) 603
Sau3AI GATC 3 cut(s) 4, 28, 355
Sau96I GGNCC 2 cut(s) 109, 196
SfaNI GCATC 1 cut(s) 156
SfcI CTRYAG 1 cut(s) 285
Sfr303I CCGCGG 1 cut(s) 603
SfuI TTCGAA 1 cut(s) 66
SgrBI CCGCGG 1 cut(s) 603
SinI GGWCC 2 cut(s) 109, 196
SmiMI CAYNNNNRTG 2 cut(s) 351, 575
SmlI CTYRAG 1 cut(s) 500
SmoI CTYRAG 1 cut(s) 500
Sse9I AATT 2 cut(s) 278, 533
SsiI CCGC 5 cut(s) 91, 220, 392, 600, 602
SspMI CTAG 1 cut(s) 679
StyI CCWWGG 2 cut(s) 39, 178
TaiI ACGT 4 cut(s) 52, 190, 408, 468
TaqI TCGA 1 cut(s) 66
TasI AATT 2 cut(s) 278, 533
TauI GCSGC 2 cut(s) 222, 602
TscAI CASTG 1 cut(s) 582
TseI GCWGC 8 cut(s) 17, 272, 284, 367, 491, 525, 528, 667
TspDTI ATGAA 2 cut(s) 444, 498
TspGWI ACGGA 1 cut(s) 137
TspRI CASTG 1 cut(s) 582
VpaK11BI GGWCC 2 cut(s) 109, 196
XapI RAATTY 1 cut(s) 278
XmiI GTMKAC 1 cut(s) 675
XspI CTAG 1 cut(s) 679
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.