RLG00000005551

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0016850
Physical Location & Seq
Reverse (-)
68492084 .. 68492865
782 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005551

Sequence Viewer

Length: 681 bp
ATGGATCAAAGCAAGGCAGCCGGAGCTGATCATCAGGCCAAACCTACTTATGAGGACTTTGAACCCTTATGCAATTGGCATAACCGCGACAATGTTGTTGAGGTCCATATTCAAGATTTCAGAAAGGAACACCTAAGTGTCCGTACCACTCAGGCGGGGATGCTTACAATCCATGGAGAACGTCCATTGGACCAAACTAAATCTACGTGGCGCCGTTTCCACAAAGAAATTAAGCTTCCAAACAACTGCGACAGGAATAGAGTTGCTGCAAAGTTTGCTGCAGGAGTTCTTACAATAACAATGCCTGCCAAAGTTGCACCACCTCAACCTCAATCTCCAGAAGATCAGAAACAACAGCTTAGCAGTCATGAAAAGTTACCAGAACCAAGTAACATCAATGATACTCAAACAAATAATGATAAAGCGATAGCTAAACTCATTAAACAAAATCCGGACAACAAGCCAACCACCGATGAAAATGCTGCATTGCTTGATGAAGAGGTTAAGCCAGGAAGCTGCTGCGAATTCGGGCAAAAGAGTACTTTAGCTTCCAAGAATAGGAAGAAATATACTGCAGTGAAACTCGTGTTGGTGGTTGCCGTGGTAGTCGCTTTTGGTTTCGGGGCATATATCAGATACAAACACGGCCATTACTCATATAGCTGGGAGCACACAGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HSP20

Protein Analysis

227

Amino Acids

25.53

Weight (kDa)

8.57

Isoelectric Point (pI)

40.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 32 - 109 1.5e-11 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016850)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g18340
malus_domestica MD06G1239100.v1.1
prunus_persica Prupe.5G242900_v2.0.a1
pyrus_communis pycom06g21460
rosa_chinensis RchiOBHm_Chr7g0177251
rosa_laevigata RLG00000005551
rosa_multiflora Rmu_sc0005310.1_g000013
rosa_roxburghii Rroxscaffold_3G00276010
rosa_rugosa Rorug06G0404200
rosa_samantha Rh7AG004200 Rh7BG004000 Rh7CG003900 Rh7DG003900
rosa_wichuraiana Rw7G000340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 210
AccII CGCG 1 cut(s) 87
AccIII TCCGGA 1 cut(s) 451
AciI CCGC 2 cut(s) 85, 155
AclWI GGATC 1 cut(s) 12
AcoI YGGCCR 1 cut(s) 646
AcsI RAATTY 1 cut(s) 524
AcyI GRCGYC 1 cut(s) 211
AfaI GTAC 2 cut(s) 145, 541
AfiI CCNNNNNNNGG 1 cut(s) 558
AgsI TTSAA 2 cut(s) 62, 113
AjnI CCWGG 1 cut(s) 508
AjuI GAANNNNNNNTTGG 2 cut(s) 572, 604
AleI CACNNNNGTG 1 cut(s) 135
AluBI AGCT 7 cut(s) 26, 235, 358, 431, 516, 548, 663
AluI AGCT 7 cut(s) 26, 235, 358, 431, 516, 548, 663
Alw21I GWGCWC 1 cut(s) 672
AlwI GGATC 1 cut(s) 12
Aor13HI TCCGGA 1 cut(s) 451
AoxI GGCC 2 cut(s) 36, 646
ApeKI GCWGC 6 cut(s) 17, 266, 278, 482, 516, 519
ApoI RAATTY 1 cut(s) 524
AspLEI GCGC 1 cut(s) 213
AspS9I GGNCC 2 cut(s) 103, 190
AvaII GGWCC 2 cut(s) 103, 190
BanI GGYRCC 1 cut(s) 210
BarI GAAGNNNNNNTAC 2 cut(s) 532, 564
BauI CACGAG 1 cut(s) 584
Bbv12I GWGCWC 1 cut(s) 672
BbvI GCAGC 6 cut(s) 29, 253, 265, 469, 503, 506
BceAI ACGGC 3 cut(s) 198, 584, 661
BcgI CGANNNNNNTGC 2 cut(s) 461, 495
BciT130I CCWGG 1 cut(s) 510
BclI TGATCA 1 cut(s) 28
BfaI CTAG 1 cut(s) 679
BfmI CTRYAG 2 cut(s) 279, 573
BfoI RGCGCY 1 cut(s) 214
BisI GCNGC 6 cut(s) 18, 267, 279, 483, 517, 520
BlpI GCTNAGC 1 cut(s) 359
BlsI GCNGC 6 cut(s) 19, 268, 280, 484, 518, 521
BmcAI AGTACT 1 cut(s) 541
Bme1390I CCNGG 1 cut(s) 510
Bme18I GGWCC 2 cut(s) 103, 190
BmgT120I GGNCC 2 cut(s) 103, 190
BmiI GGNNCC 1 cut(s) 212
BmrFI CCNGG 1 cut(s) 510
BmsI GCATC 1 cut(s) 150
BpmI CTGGAG 1 cut(s) 321
Bpu1102I GCTNAGC 1 cut(s) 359
BsaAI YACGTR 1 cut(s) 207
BsaHI GRCGYC 1 cut(s) 211
BsaJI CCNNGG 2 cut(s) 172, 600
BsaWI WCCGGW 1 cut(s) 451
Bsc4I CCNNNNNNNGG 1 cut(s) 558
Bse3DI GCAATG 1 cut(s) 485
BseAI TCCGGA 1 cut(s) 451
BseBI CCWGG 1 cut(s) 510
BseDI CCNNGG 2 cut(s) 172, 600
BseGI GGATG 1 cut(s) 165
BseLI CCNNNNNNNGG 1 cut(s) 558
BseMI GCAATG 1 cut(s) 485
BseMII CTCAG 1 cut(s) 164
BseXI GCAGC 6 cut(s) 29, 253, 265, 469, 503, 506
BseYI CCCAGC 1 cut(s) 663
Bsh1236I CGCG 1 cut(s) 87
BshFI GGCC 2 cut(s) 38, 648
BshNI GGYRCC 1 cut(s) 210
BsiHKAI GWGCWC 1 cut(s) 672
BsiSI CCGG 2 cut(s) 21, 452
BslI CCNNNNNNNGG 1 cut(s) 558
BsnI GGCC 2 cut(s) 38, 648
Bsp1286I GDGCHC 1 cut(s) 672
Bsp13I TCCGGA 1 cut(s) 451
Bsp143I GATC 3 cut(s) 4, 28, 343
Bsp1720I GCTNAGC 1 cut(s) 359
Bsp19I CCATGG 1 cut(s) 172
BspACI CCGC 2 cut(s) 85, 155
BspANI GGCC 2 cut(s) 38, 648
BspCNI CTCAG 1 cut(s) 163
BspEI TCCGGA 1 cut(s) 451
BspFNI CGCG 1 cut(s) 87
BspHI TCATGA 1 cut(s) 367
BspLI GGNNCC 1 cut(s) 212
BspMAI CTGCAG 2 cut(s) 283, 577
BspPI GGATC 1 cut(s) 12
BspT107I GGYRCC 1 cut(s) 210
BsrDI GCAATG 1 cut(s) 485
BssECI CCNNGG 2 cut(s) 172, 600
BssMI GATC 3 cut(s) 4, 28, 343
BssNI GRCGYC 1 cut(s) 211
BssSI CACGAG 1 cut(s) 584
BssT1I CCWWGG 1 cut(s) 172
Bst2BI CACGAG 1 cut(s) 584
Bst2UI CCWGG 1 cut(s) 510
Bst6I CTCTTC 1 cut(s) 492
BstACI GRCGYC 1 cut(s) 211
BstAPI GCANNNNNTGC 1 cut(s) 275
BstBAI YACGTR 1 cut(s) 207
BstC8I GCNNGC 1 cut(s) 306
BstDEI CTNAG 3 cut(s) 134, 150, 359
BstDSI CCRYGG 2 cut(s) 172, 600
BstF5I GGATG 1 cut(s) 165
BstFNI CGCG 1 cut(s) 87
BstH2I RGCGCY 1 cut(s) 214
BstHHI GCGC 1 cut(s) 213
BstKTI GATC 3 cut(s) 7, 31, 346
BstMBI GATC 3 cut(s) 4, 28, 343
BstMWI GCNNNNNNNGC 3 cut(s) 23, 275, 314
BstNI CCWGG 1 cut(s) 510
BstSCI CCNGG 1 cut(s) 508
BstSFI CTRYAG 2 cut(s) 279, 573
BstUI CGCG 1 cut(s) 87
BstV1I GCAGC 6 cut(s) 29, 253, 265, 469, 503, 506
BsuRI GGCC 2 cut(s) 38, 648
BtgI CCRYGG 2 cut(s) 172, 600
BtsCI GGATG 1 cut(s) 165
BtsI GCAGTG 1 cut(s) 582
BtsIMutI CAGTG 1 cut(s) 582
Cac8I GCNNGC 1 cut(s) 306
CciI TCATGA 1 cut(s) 367
CfoI GCGC 1 cut(s) 213
Cfr13I GGNCC 2 cut(s) 103, 190
Csp6I GTAC 2 cut(s) 144, 540
CviAII CATG 2 cut(s) 173, 368
CviQI GTAC 2 cut(s) 144, 540
DdeI CTNAG 3 cut(s) 134, 150, 359
DinI GGCGCC 1 cut(s) 212
DpnI GATC 3 cut(s) 6, 30, 345
DpnII GATC 3 cut(s) 4, 28, 343
EaeI YGGCCR 1 cut(s) 646
Eam1104I CTCTTC 1 cut(s) 492
EarI CTCTTC 1 cut(s) 492
Eco130I CCWWGG 1 cut(s) 172
Eco47I GGWCC 2 cut(s) 103, 190
EcoRI GAATTC 1 cut(s) 524
EcoRII CCWGG 1 cut(s) 508
EcoT14I CCWWGG 1 cut(s) 172
EgeI GGCGCC 1 cut(s) 212
EheI GGCGCC 1 cut(s) 212
ErhI CCWWGG 1 cut(s) 172
FaeI CATG 2 cut(s) 176, 371
FatI CATG 2 cut(s) 172, 367
FauI CCCGC 1 cut(s) 148
FbaI TGATCA 1 cut(s) 28
Fnu4HI GCNGC 6 cut(s) 18, 267, 279, 483, 517, 520
FokI GGATG 1 cut(s) 172
Fsp4HI GCNGC 6 cut(s) 18, 267, 279, 483, 517, 520
FspBI CTAG 1 cut(s) 679
GlaI GCGC 1 cut(s) 212
GluI GCNGC 6 cut(s) 18, 267, 279, 483, 517, 520
GsaI CCCAGC 1 cut(s) 667
GsuI CTGGAG 1 cut(s) 321
HaeII RGCGCY 1 cut(s) 214
HaeIII GGCC 2 cut(s) 38, 648
HapII CCGG 2 cut(s) 21, 452
HhaI GCGC 1 cut(s) 213
Hin1I GRCGYC 1 cut(s) 211
Hin1II CATG 2 cut(s) 176, 371
Hin6I GCGC 1 cut(s) 211
HinP1I GCGC 1 cut(s) 211
HindIII AAGCTT 1 cut(s) 233
HpaII CCGG 2 cut(s) 21, 452
Hpy188I TCNGA 3 cut(s) 122, 348, 635
Hpy188III TCNNGA 4 cut(s) 113, 338, 368, 452
HpyCH4IV ACGT 2 cut(s) 181, 206
HpyCH4V TGCA 6 cut(s) 72, 269, 281, 317, 485, 575
HpyF10VI GCNNNNNNNGC 3 cut(s) 23, 275, 314
HpyF3I CTNAG 3 cut(s) 134, 150, 359
HpySE526I ACGT 2 cut(s) 181, 206
Hsp92I GRCGYC 1 cut(s) 211
Hsp92II CATG 2 cut(s) 176, 371
HspAI GCGC 1 cut(s) 211
KasI GGCGCC 1 cut(s) 210
Kpn2I TCCGGA 1 cut(s) 451
Ksp22I TGATCA 1 cut(s) 28
Kzo9I GATC 3 cut(s) 4, 28, 343
LmnI GCTCC 2 cut(s) 23, 667
Lsp1109I GCAGC 6 cut(s) 29, 253, 265, 469, 503, 506
LweI GCATC 1 cut(s) 150
MaeI CTAG 1 cut(s) 679
MaeII ACGT 2 cut(s) 181, 206
MaeIII GTNAC 2 cut(s) 375, 389
MalI GATC 3 cut(s) 6, 30, 345
MboI GATC 3 cut(s) 4, 28, 343
MboII GAAGA 3 cut(s) 353, 509, 574
MfeI CAATTG 1 cut(s) 73
MhlI GDGCHC 1 cut(s) 672
MluCI AATT 3 cut(s) 73, 228, 524
Mly113I GGCGCC 1 cut(s) 211
MnlI CCTC 5 cut(s) 46, 94, 333, 339, 493
MroI TCCGGA 1 cut(s) 451
MseI TTAA 3 cut(s) 231, 441, 504
MslI CAYNNNNRTG 1 cut(s) 135
MspI CCGG 2 cut(s) 21, 452
MspR9I CCNGG 1 cut(s) 510
MunI CAATTG 1 cut(s) 73
MvaI CCWGG 1 cut(s) 510
MvnI CGCG 1 cut(s) 87
MwoI GCNNNNNNNGC 3 cut(s) 23, 275, 314
NarI GGCGCC 1 cut(s) 211
NcoI CCATGG 1 cut(s) 172
NdeII GATC 3 cut(s) 4, 28, 343
NlaIII CATG 2 cut(s) 176, 371
NlaIV GGNNCC 1 cut(s) 212
OliI CACNNNNGTG 1 cut(s) 135
PagI TCATGA 1 cut(s) 367
PkrI GCNGC 6 cut(s) 19, 268, 280, 484, 518, 521
PluTI GGCGCC 1 cut(s) 214
Ppu21I YACGTR 1 cut(s) 207
Psp6I CCWGG 1 cut(s) 508
PspFI CCCAGC 1 cut(s) 663
PspGI CCWGG 1 cut(s) 508
PspN4I GGNNCC 1 cut(s) 212
PspPI GGNCC 2 cut(s) 103, 190
PstI CTGCAG 2 cut(s) 283, 577
RsaI GTAC 2 cut(s) 145, 541
RsaNI GTAC 2 cut(s) 144, 540
RseI CAYNNNNRTG 1 cut(s) 135
SaqAI TTAA 3 cut(s) 231, 441, 504
SatI GCNGC 6 cut(s) 18, 267, 279, 483, 517, 520
Sau3AI GATC 3 cut(s) 4, 28, 343
Sau96I GGNCC 2 cut(s) 103, 190
ScaI AGTACT 1 cut(s) 541
ScrFI CCNGG 1 cut(s) 510
SduI GDGCHC 1 cut(s) 672
SfaNI GCATC 1 cut(s) 150
SfcI CTRYAG 2 cut(s) 279, 573
SfoI GGCGCC 1 cut(s) 212
SinI GGWCC 2 cut(s) 103, 190
SmiMI CAYNNNNRTG 1 cut(s) 135
Sse9I AATT 3 cut(s) 73, 228, 524
SsiI CCGC 2 cut(s) 85, 155
SspDI GGCGCC 1 cut(s) 210
SspMI CTAG 1 cut(s) 679
StyD4I CCNGG 1 cut(s) 508
StyI CCWWGG 1 cut(s) 172
TaiI ACGT 2 cut(s) 184, 209
TasI AATT 3 cut(s) 73, 228, 524
TatI WGTACW 1 cut(s) 539
Tru1I TTAA 3 cut(s) 231, 441, 504
Tru9I TTAA 3 cut(s) 231, 441, 504
TscAI CASTG 1 cut(s) 582
TseI GCWGC 6 cut(s) 17, 266, 278, 482, 516, 519
TspDTI ATGAA 3 cut(s) 384, 489, 510
TspGWI ACGGA 1 cut(s) 131
TspRI CASTG 1 cut(s) 582
VpaK11BI GGWCC 2 cut(s) 103, 190
XapI RAATTY 1 cut(s) 524
XspI CTAG 1 cut(s) 679
ZrmI AGTACT 1 cut(s) 541
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.