RchiOBHm_Chr7g0177251

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0016850
Physical Location & Seq
Forward (+)
304044 .. 305008
965 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ15787

Sequence Viewer

Length: 666 bp
ATGGATCAAAGCAAGGTAGCCGGAGCTGATCATCAGGCCAAACGTACTTACGAGGACTTTGAACCCTTCTGCAAATGGCATAACCGCGACAATGTTGTTGAGGTCCATATTCAAGGTTTCAGAAAGGAACACCTAAGTGTCCGTACCACTCAGACGGGGATGCTTACAATCCATGGAGAACGTCCATTGGACCAAACTAAATCTACATGGAGCCGTTTCCACAAAGAAATTAAGCTTCCAAACAACTGCGACACAAATAAAGTTGCGGCAAAGTTTGCTGCAGGAGTTCTTACAATAACAATGCCTCCCAAAATTGCACCACCTCAACCTCAATCTCCAGAACATCAGAAACAACAGCTTAGCAATCATGAAAAGTTACCAGAACCAAGCAACAATGATACTCAAACAAATAATGATAAAGCGATAGCTAAACTCATTAAACAAAATCCGGACAAGCAGCCAACCACCGATGAAAATGCTGCATTGCTTGATGAAGAGGTTAAGCCAGGAAGCTGCTGCGAATTGGGGCAAAAGAGTACTTTAGCTTCCAAGAATTGGAAGGTGAAACTCGTGTTGGTGGTTGCCGTGGTGGTCGCTTTTGGTTTCGGGGCATATATCAGATACAAACACGGCCATTACTCATACAGCTGGGAGCAGACAGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HSP20

Protein Analysis

221

Amino Acids

25.0

Weight (kDa)

8.57

Isoelectric Point (pI)

38.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 32 - 109 1.2e-12 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016850)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g18340
malus_domestica MD06G1239100.v1.1
prunus_persica Prupe.5G242900_v2.0.a1
pyrus_communis pycom06g21460
rosa_chinensis RchiOBHm_Chr7g0177251
rosa_laevigata RLG00000005551
rosa_multiflora Rmu_sc0005310.1_g000013
rosa_roxburghii Rroxscaffold_3G00276010
rosa_rugosa Rorug06G0404200
rosa_samantha Rh7AG004200 Rh7BG004000 Rh7CG003900 Rh7DG003900
rosa_wichuraiana Rw7G000340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 555
AccII CGCG 1 cut(s) 87
AccIII TCCGGA 1 cut(s) 448
AciI CCGC 2 cut(s) 85, 266
AclWI GGATC 1 cut(s) 12
AcoI YGGCCR 1 cut(s) 631
AfaI GTAC 3 cut(s) 46, 145, 538
AfiI CCNNNNNNNGG 1 cut(s) 555
AgsI TTSAA 2 cut(s) 62, 113
AjnI CCWGG 1 cut(s) 505
AjuI GAANNNNNNNTTGG 2 cut(s) 557, 589
AleI CACNNNNGTG 1 cut(s) 135
AluBI AGCT 7 cut(s) 26, 235, 358, 428, 513, 545, 648
AluI AGCT 7 cut(s) 26, 235, 358, 428, 513, 545, 648
AlwI GGATC 1 cut(s) 12
Aor13HI TCCGGA 1 cut(s) 448
AoxI GGCC 2 cut(s) 36, 631
ApeKI GCWGC 5 cut(s) 278, 457, 479, 513, 516
AspS9I GGNCC 2 cut(s) 103, 190
AsuHPI GGTGA 1 cut(s) 574
AvaII GGWCC 2 cut(s) 103, 190
BarI GAAGNNNNNNTAC 2 cut(s) 529, 561
BauI CACGAG 1 cut(s) 569
BbvI GCAGC 5 cut(s) 265, 466, 469, 500, 503
BceAI ACGGC 3 cut(s) 198, 569, 646
BcgI CGANNNNNNTGC 2 cut(s) 458, 492
BciT130I CCWGG 1 cut(s) 507
BclI TGATCA 1 cut(s) 28
BfaI CTAG 1 cut(s) 664
BfmI CTRYAG 1 cut(s) 279
BisI GCNGC 6 cut(s) 267, 279, 458, 480, 514, 517
BlpI GCTNAGC 1 cut(s) 359
BlsI GCNGC 6 cut(s) 268, 280, 459, 481, 515, 518
BmcAI AGTACT 1 cut(s) 538
Bme1390I CCNGG 1 cut(s) 507
Bme18I GGWCC 2 cut(s) 103, 190
BmgT120I GGNCC 2 cut(s) 103, 190
BmiI GGNNCC 1 cut(s) 212
BmrFI CCNGG 1 cut(s) 507
BmsI GCATC 1 cut(s) 150
BpmI CTGGAG 1 cut(s) 321
Bpu1102I GCTNAGC 1 cut(s) 359
BsaJI CCNNGG 2 cut(s) 172, 585
BsaWI WCCGGW 1 cut(s) 448
BsaXI ACNNNNNCTCC 2 cut(s) 289, 319
Bsc4I CCNNNNNNNGG 1 cut(s) 555
Bse3DI GCAATG 1 cut(s) 482
BseAI TCCGGA 1 cut(s) 448
BseBI CCWGG 1 cut(s) 507
BseDI CCNNGG 2 cut(s) 172, 585
BseGI GGATG 1 cut(s) 165
BseLI CCNNNNNNNGG 1 cut(s) 555
BseMI GCAATG 1 cut(s) 482
BseMII CTCAG 1 cut(s) 164
BseXI GCAGC 5 cut(s) 265, 466, 469, 500, 503
BseYI CCCAGC 1 cut(s) 648
Bsh1236I CGCG 1 cut(s) 87
BshFI GGCC 2 cut(s) 38, 633
BsiSI CCGG 2 cut(s) 21, 449
BslI CCNNNNNNNGG 1 cut(s) 555
BsnI GGCC 2 cut(s) 38, 633
Bsp13I TCCGGA 1 cut(s) 448
Bsp143I GATC 2 cut(s) 4, 28
Bsp1720I GCTNAGC 1 cut(s) 359
Bsp19I CCATGG 1 cut(s) 172
BspACI CCGC 2 cut(s) 85, 266
BspANI GGCC 2 cut(s) 38, 633
BspCNI CTCAG 1 cut(s) 163
BspEI TCCGGA 1 cut(s) 448
BspFNI CGCG 1 cut(s) 87
BspHI TCATGA 1 cut(s) 367
BspLI GGNNCC 1 cut(s) 212
BspMAI CTGCAG 1 cut(s) 283
BspPI GGATC 1 cut(s) 12
BsrDI GCAATG 1 cut(s) 482
BssECI CCNNGG 2 cut(s) 172, 585
BssMI GATC 2 cut(s) 4, 28
BssSI CACGAG 1 cut(s) 569
BssT1I CCWWGG 1 cut(s) 172
Bst2BI CACGAG 1 cut(s) 569
Bst2UI CCWGG 1 cut(s) 507
Bst6I CTCTTC 1 cut(s) 489
BstAPI GCANNNNNTGC 1 cut(s) 275
BstDEI CTNAG 3 cut(s) 134, 150, 359
BstDSI CCRYGG 2 cut(s) 172, 585
BstF5I GGATG 1 cut(s) 165
BstFNI CGCG 1 cut(s) 87
BstKTI GATC 2 cut(s) 7, 31
BstMBI GATC 2 cut(s) 4, 28
BstMWI GCNNNNNNNGC 1 cut(s) 275
BstNI CCWGG 1 cut(s) 507
BstSCI CCNGG 1 cut(s) 505
BstSFI CTRYAG 1 cut(s) 279
BstUI CGCG 1 cut(s) 87
BstV1I GCAGC 5 cut(s) 265, 466, 469, 500, 503
BsuRI GGCC 2 cut(s) 38, 633
BtgI CCRYGG 2 cut(s) 172, 585
BtsCI GGATG 1 cut(s) 165
CciI TCATGA 1 cut(s) 367
Cfr13I GGNCC 2 cut(s) 103, 190
Csp6I GTAC 3 cut(s) 45, 144, 537
CviAII CATG 3 cut(s) 173, 207, 368
CviQI GTAC 3 cut(s) 45, 144, 537
DdeI CTNAG 3 cut(s) 134, 150, 359
DpnI GATC 2 cut(s) 6, 30
DpnII GATC 2 cut(s) 4, 28
EaeI YGGCCR 1 cut(s) 631
Eam1104I CTCTTC 1 cut(s) 489
EarI CTCTTC 1 cut(s) 489
Eco130I CCWWGG 1 cut(s) 172
Eco47I GGWCC 2 cut(s) 103, 190
EcoRII CCWGG 1 cut(s) 505
EcoT14I CCWWGG 1 cut(s) 172
ErhI CCWWGG 1 cut(s) 172
FaeI CATG 3 cut(s) 176, 210, 371
FaiI YATR 8 cut(s) 81, 108, 174, 208, 369, 613, 615, 643
FatI CATG 3 cut(s) 172, 206, 367
FbaI TGATCA 1 cut(s) 28
Fnu4HI GCNGC 6 cut(s) 267, 279, 458, 480, 514, 517
FokI GGATG 1 cut(s) 172
Fsp4HI GCNGC 6 cut(s) 267, 279, 458, 480, 514, 517
FspBI CTAG 1 cut(s) 664
GluI GCNGC 6 cut(s) 267, 279, 458, 480, 514, 517
GsaI CCCAGC 1 cut(s) 652
GsuI CTGGAG 1 cut(s) 321
HaeIII GGCC 2 cut(s) 38, 633
HapII CCGG 2 cut(s) 21, 449
Hin1II CATG 3 cut(s) 176, 210, 371
HindIII AAGCTT 1 cut(s) 233
HpaII CCGG 2 cut(s) 21, 449
HphI GGTGA 1 cut(s) 574
Hpy188I TCNGA 4 cut(s) 122, 153, 348, 620
Hpy188III TCNNGA 3 cut(s) 338, 368, 449
HpyAV CCTTC 2 cut(s) 76, 553
HpyCH4IV ACGT 2 cut(s) 43, 181
HpyCH4V TGCA 4 cut(s) 72, 281, 317, 482
HpyF10VI GCNNNNNNNGC 1 cut(s) 275
HpyF3I CTNAG 3 cut(s) 134, 150, 359
HpySE526I ACGT 2 cut(s) 43, 181
Hsp92II CATG 3 cut(s) 176, 210, 371
Kpn2I TCCGGA 1 cut(s) 448
Ksp22I TGATCA 1 cut(s) 28
Kzo9I GATC 2 cut(s) 4, 28
LmnI GCTCC 3 cut(s) 23, 210, 652
LpnPI CCDG 9 cut(s) 20, 34, 267, 351, 393, 462, 492, 519, 634
Lsp1109I GCAGC 5 cut(s) 265, 466, 469, 500, 503
LweI GCATC 1 cut(s) 150
MaeI CTAG 1 cut(s) 664
MaeII ACGT 2 cut(s) 43, 181
MaeIII GTNAC 1 cut(s) 375
MalI GATC 2 cut(s) 6, 30
MboI GATC 2 cut(s) 4, 28
MboII GAAGA 1 cut(s) 506
MluCI AATT 4 cut(s) 228, 312, 521, 553
MnlI CCTC 6 cut(s) 46, 94, 315, 333, 339, 490
MroI TCCGGA 1 cut(s) 448
MseI TTAA 3 cut(s) 231, 438, 501
MslI CAYNNNNRTG 1 cut(s) 135
MspA1I CMGCKG 1 cut(s) 648
MspI CCGG 2 cut(s) 21, 449
MspR9I CCNGG 1 cut(s) 507
MvaI CCWGG 1 cut(s) 507
MvnI CGCG 1 cut(s) 87
MwoI GCNNNNNNNGC 1 cut(s) 275
NcoI CCATGG 1 cut(s) 172
NdeII GATC 2 cut(s) 4, 28
NlaIII CATG 3 cut(s) 176, 210, 371
NlaIV GGNNCC 1 cut(s) 212
OliI CACNNNNGTG 1 cut(s) 135
PagI TCATGA 1 cut(s) 367
PflMI CCANNNNNTGG 1 cut(s) 555
PkrI GCNGC 6 cut(s) 268, 280, 459, 481, 515, 518
Psp6I CCWGG 1 cut(s) 505
PspFI CCCAGC 1 cut(s) 648
PspGI CCWGG 1 cut(s) 505
PspN4I GGNNCC 1 cut(s) 212
PspPI GGNCC 2 cut(s) 103, 190
PstI CTGCAG 1 cut(s) 283
PvuII CAGCTG 1 cut(s) 648
RsaI GTAC 3 cut(s) 46, 145, 538
RsaNI GTAC 3 cut(s) 45, 144, 537
RseI CAYNNNNRTG 1 cut(s) 135
SaqAI TTAA 3 cut(s) 231, 438, 501
SatI GCNGC 6 cut(s) 267, 279, 458, 480, 514, 517
Sau3AI GATC 2 cut(s) 4, 28
Sau96I GGNCC 2 cut(s) 103, 190
ScaI AGTACT 1 cut(s) 538
ScrFI CCNGG 1 cut(s) 507
SfaNI GCATC 1 cut(s) 150
SfcI CTRYAG 1 cut(s) 279
SinI GGWCC 2 cut(s) 103, 190
SmiMI CAYNNNNRTG 1 cut(s) 135
Sse9I AATT 4 cut(s) 228, 312, 521, 553
SsiI CCGC 2 cut(s) 85, 266
SspMI CTAG 1 cut(s) 664
StyD4I CCNGG 1 cut(s) 505
StyI CCWWGG 1 cut(s) 172
TaiI ACGT 2 cut(s) 46, 184
TasI AATT 4 cut(s) 228, 312, 521, 553
TatI WGTACW 1 cut(s) 536
TauI GCSGC 1 cut(s) 269
Tru1I TTAA 3 cut(s) 231, 438, 501
Tru9I TTAA 3 cut(s) 231, 438, 501
TseI GCWGC 5 cut(s) 278, 457, 479, 513, 516
TspDTI ATGAA 3 cut(s) 384, 486, 507
TspGWI ACGGA 1 cut(s) 131
Van91I CCANNNNNTGG 1 cut(s) 555
VpaK11BI GGWCC 2 cut(s) 103, 190
XspI CTAG 1 cut(s) 664
ZrmI AGTACT 1 cut(s) 538
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.