Rw7G000340

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0016850
Physical Location & Seq
Forward (+)
290204 .. 291155
952 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G000340.1

Sequence Viewer

Length: 666 bp
ATGGCTCAAAGCAAGGCAGCCGGAGCTGATCATCAGGCCAAACGTACTTACGAGGACTTTGAACCCTTCTGCAAATGGCATAACCGCGACAATGTTGTTGAGGTCCATATTCAAGGTTTCAGAAAGGAACACCTAAGTGTCCGTACCACTCAGACGGGGATGCTTACAATCCATGGAGAACGTCCACTGGATCAAACTAAATCTACATGGAGCCGTTTCCACAAAGAAATTAAGCTTCCAAACAACTGCGACACAAATAGAGTTGCGGCAAAGTTTGCTGCAGGAGTTCTTACAGTAACAATGCCTCCCAAAATTGCACCACCTCAACCTCAATCTCCAGAAGATCAGAAACAACAGCTTAGCAATCATGAAAAGTTACCAGAACCAAGCAACAATGATACTCAAACAAATAATGATAAAGCGATAGCTAAGCTCGTTAAACAAAATCCGGACAAGCAGCCAACCACCGATGAAAATGCTGCATTGCTTGATGAAGAGGTTAAGCCAGAAAGCTGCTGCGAATTGGGGCAAAAGAGTGCTTTAGCTTACAAGAATTGGAAGGTGAAACTCGTGTTGGTGGTTGCCGTGGTGGTCGCTTTTGGTTTCGGGGCATATATCAGATACAAACACGGCCATTACTCATACAGCTGGGAGCAGACAGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HSP20

Protein Analysis

221

Amino Acids

25.02

Weight (kDa)

8.26

Isoelectric Point (pI)

41.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 32 - 109 2.4e-12 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016850)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g18340
malus_domestica MD06G1239100.v1.1
prunus_persica Prupe.5G242900_v2.0.a1
pyrus_communis pycom06g21460
rosa_chinensis RchiOBHm_Chr7g0177251
rosa_laevigata RLG00000005551
rosa_multiflora Rmu_sc0005310.1_g000013
rosa_roxburghii Rroxscaffold_3G00276010
rosa_rugosa Rorug06G0404200
rosa_samantha Rh7AG004200 Rh7BG004000 Rh7CG003900 Rh7DG003900
rosa_wichuraiana Rw7G000340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 87
AccIII TCCGGA 1 cut(s) 448
AciI CCGC 2 cut(s) 85, 266
AclWI GGATC 1 cut(s) 198
AcoI YGGCCR 1 cut(s) 631
AfaI GTAC 2 cut(s) 46, 145
AgsI TTSAA 2 cut(s) 62, 113
AjuI GAANNNNNNNTTGG 2 cut(s) 557, 589
AleI CACNNNNGTG 1 cut(s) 135
AluBI AGCT 8 cut(s) 26, 235, 358, 428, 433, 513, 545, 648
AluI AGCT 8 cut(s) 26, 235, 358, 428, 433, 513, 545, 648
AlwI GGATC 1 cut(s) 198
Aor13HI TCCGGA 1 cut(s) 448
AoxI GGCC 2 cut(s) 36, 631
ApeKI GCWGC 6 cut(s) 17, 278, 457, 479, 513, 516
AspS9I GGNCC 1 cut(s) 103
AsuHPI GGTGA 1 cut(s) 574
AvaII GGWCC 1 cut(s) 103
BauI CACGAG 1 cut(s) 569
BbvI GCAGC 6 cut(s) 29, 265, 466, 469, 500, 503
BceAI ACGGC 3 cut(s) 198, 569, 646
BcgI CGANNNNNNTGC 2 cut(s) 458, 492
BclI TGATCA 1 cut(s) 28
BfaI CTAG 1 cut(s) 664
BfmI CTRYAG 1 cut(s) 279
BisI GCNGC 7 cut(s) 18, 267, 279, 458, 480, 514, 517
BlpI GCTNAGC 2 cut(s) 359, 429
BlsI GCNGC 7 cut(s) 19, 268, 280, 459, 481, 515, 518
Bme18I GGWCC 1 cut(s) 103
BmgT120I GGNCC 1 cut(s) 103
BmiI GGNNCC 1 cut(s) 212
BmsI GCATC 1 cut(s) 150
BpmI CTGGAG 1 cut(s) 321
Bpu1102I GCTNAGC 2 cut(s) 359, 429
BsaJI CCNNGG 2 cut(s) 172, 585
BsaWI WCCGGW 1 cut(s) 448
BsaXI ACNNNNNCTCC 2 cut(s) 289, 319
Bse1I ACTGG 1 cut(s) 192
Bse3DI GCAATG 1 cut(s) 482
BseAI TCCGGA 1 cut(s) 448
BseDI CCNNGG 2 cut(s) 172, 585
BseGI GGATG 1 cut(s) 165
BseMI GCAATG 1 cut(s) 482
BseMII CTCAG 1 cut(s) 164
BseNI ACTGG 1 cut(s) 192
BseXI GCAGC 6 cut(s) 29, 265, 466, 469, 500, 503
BseYI CCCAGC 1 cut(s) 648
Bsh1236I CGCG 1 cut(s) 87
BshFI GGCC 2 cut(s) 38, 633
BsiSI CCGG 2 cut(s) 21, 449
BsnI GGCC 2 cut(s) 38, 633
Bsp13I TCCGGA 1 cut(s) 448
Bsp143I GATC 3 cut(s) 28, 190, 343
Bsp1720I GCTNAGC 2 cut(s) 359, 429
Bsp19I CCATGG 1 cut(s) 172
BspACI CCGC 2 cut(s) 85, 266
BspANI GGCC 2 cut(s) 38, 633
BspCNI CTCAG 1 cut(s) 163
BspEI TCCGGA 1 cut(s) 448
BspFNI CGCG 1 cut(s) 87
BspHI TCATGA 1 cut(s) 367
BspLI GGNNCC 1 cut(s) 212
BspMAI CTGCAG 1 cut(s) 283
BspPI GGATC 1 cut(s) 198
BsrDI GCAATG 1 cut(s) 482
BsrI ACTGG 1 cut(s) 192
BssECI CCNNGG 2 cut(s) 172, 585
BssMI GATC 3 cut(s) 28, 190, 343
BssSI CACGAG 1 cut(s) 569
BssT1I CCWWGG 1 cut(s) 172
Bst2BI CACGAG 1 cut(s) 569
Bst4CI ACNGT 1 cut(s) 295
Bst6I CTCTTC 1 cut(s) 489
BstAPI GCANNNNNTGC 1 cut(s) 275
BstDEI CTNAG 4 cut(s) 134, 150, 359, 429
BstDSI CCRYGG 2 cut(s) 172, 585
BstF5I GGATG 1 cut(s) 165
BstFNI CGCG 1 cut(s) 87
BstKTI GATC 3 cut(s) 31, 193, 346
BstMBI GATC 3 cut(s) 28, 190, 343
BstMWI GCNNNNNNNGC 2 cut(s) 23, 275
BstSFI CTRYAG 1 cut(s) 279
BstUI CGCG 1 cut(s) 87
BstV1I GCAGC 6 cut(s) 29, 265, 466, 469, 500, 503
BsuRI GGCC 2 cut(s) 38, 633
BtgI CCRYGG 2 cut(s) 172, 585
BtsCI GGATG 1 cut(s) 165
BtsIMutI CAGTG 1 cut(s) 185
CciI TCATGA 1 cut(s) 367
Cfr13I GGNCC 1 cut(s) 103
Csp6I GTAC 2 cut(s) 45, 144
CviAII CATG 3 cut(s) 173, 207, 368
CviQI GTAC 2 cut(s) 45, 144
DdeI CTNAG 4 cut(s) 134, 150, 359, 429
DpnI GATC 3 cut(s) 30, 192, 345
DpnII GATC 3 cut(s) 28, 190, 343
EaeI YGGCCR 1 cut(s) 631
Eam1104I CTCTTC 1 cut(s) 489
EarI CTCTTC 1 cut(s) 489
Eco130I CCWWGG 1 cut(s) 172
Eco47I GGWCC 1 cut(s) 103
EcoT14I CCWWGG 1 cut(s) 172
ErhI CCWWGG 1 cut(s) 172
FaeI CATG 3 cut(s) 176, 210, 371
FaiI YATR 8 cut(s) 81, 108, 174, 208, 369, 613, 615, 643
FatI CATG 3 cut(s) 172, 206, 367
FbaI TGATCA 1 cut(s) 28
Fnu4HI GCNGC 7 cut(s) 18, 267, 279, 458, 480, 514, 517
FokI GGATG 1 cut(s) 172
Fsp4HI GCNGC 7 cut(s) 18, 267, 279, 458, 480, 514, 517
FspBI CTAG 1 cut(s) 664
GluI GCNGC 7 cut(s) 18, 267, 279, 458, 480, 514, 517
GsaI CCCAGC 1 cut(s) 652
GsuI CTGGAG 1 cut(s) 321
HaeIII GGCC 2 cut(s) 38, 633
HapII CCGG 2 cut(s) 21, 449
Hin1II CATG 3 cut(s) 176, 210, 371
HindIII AAGCTT 1 cut(s) 233
HpaII CCGG 2 cut(s) 21, 449
HphI GGTGA 1 cut(s) 574
Hpy166II GTNNAC 1 cut(s) 185
Hpy188I TCNGA 4 cut(s) 122, 153, 348, 620
Hpy188III TCNNGA 3 cut(s) 338, 368, 449
Hpy8I GTNNAC 1 cut(s) 185
HpyAV CCTTC 2 cut(s) 76, 553
HpyCH4III ACNGT 1 cut(s) 295
HpyCH4IV ACGT 2 cut(s) 43, 181
HpyCH4V TGCA 4 cut(s) 72, 281, 317, 482
HpyF10VI GCNNNNNNNGC 2 cut(s) 23, 275
HpyF3I CTNAG 4 cut(s) 134, 150, 359, 429
HpySE526I ACGT 2 cut(s) 43, 181
Hsp92II CATG 3 cut(s) 176, 210, 371
Kpn2I TCCGGA 1 cut(s) 448
Ksp22I TGATCA 1 cut(s) 28
Kzo9I GATC 3 cut(s) 28, 190, 343
LmnI GCTCC 3 cut(s) 23, 210, 652
LpnPI CCDG 9 cut(s) 20, 34, 173, 267, 351, 393, 462, 519, 634
Lsp1109I GCAGC 6 cut(s) 29, 265, 466, 469, 500, 503
LweI GCATC 1 cut(s) 150
MaeI CTAG 1 cut(s) 664
MaeII ACGT 2 cut(s) 43, 181
MaeIII GTNAC 2 cut(s) 295, 375
MalI GATC 3 cut(s) 30, 192, 345
MboI GATC 3 cut(s) 28, 190, 343
MboII GAAGA 2 cut(s) 353, 506
MluCI AATT 4 cut(s) 228, 312, 521, 553
MnlI CCTC 6 cut(s) 46, 94, 315, 333, 339, 490
MroI TCCGGA 1 cut(s) 448
MseI TTAA 3 cut(s) 231, 438, 501
MslI CAYNNNNRTG 1 cut(s) 135
MspA1I CMGCKG 1 cut(s) 648
MspI CCGG 2 cut(s) 21, 449
MvnI CGCG 1 cut(s) 87
MwoI GCNNNNNNNGC 2 cut(s) 23, 275
NcoI CCATGG 1 cut(s) 172
NdeII GATC 3 cut(s) 28, 190, 343
NlaIII CATG 3 cut(s) 176, 210, 371
NlaIV GGNNCC 1 cut(s) 212
OliI CACNNNNGTG 1 cut(s) 135
PagI TCATGA 1 cut(s) 367
PkrI GCNGC 7 cut(s) 19, 268, 280, 459, 481, 515, 518
PspFI CCCAGC 1 cut(s) 648
PspN4I GGNNCC 1 cut(s) 212
PspPI GGNCC 1 cut(s) 103
PstI CTGCAG 1 cut(s) 283
PvuII CAGCTG 1 cut(s) 648
RsaI GTAC 2 cut(s) 46, 145
RsaNI GTAC 2 cut(s) 45, 144
RseI CAYNNNNRTG 1 cut(s) 135
SaqAI TTAA 3 cut(s) 231, 438, 501
SatI GCNGC 7 cut(s) 18, 267, 279, 458, 480, 514, 517
Sau3AI GATC 3 cut(s) 28, 190, 343
Sau96I GGNCC 1 cut(s) 103
SfaNI GCATC 1 cut(s) 150
SfcI CTRYAG 1 cut(s) 279
SinI GGWCC 1 cut(s) 103
SmiMI CAYNNNNRTG 1 cut(s) 135
Sse9I AATT 4 cut(s) 228, 312, 521, 553
SsiI CCGC 2 cut(s) 85, 266
SspMI CTAG 1 cut(s) 664
StyI CCWWGG 1 cut(s) 172
TaaI ACNGT 1 cut(s) 295
TaiI ACGT 2 cut(s) 46, 184
TasI AATT 4 cut(s) 228, 312, 521, 553
TauI GCSGC 1 cut(s) 269
Tru1I TTAA 3 cut(s) 231, 438, 501
Tru9I TTAA 3 cut(s) 231, 438, 501
TscAI CASTG 1 cut(s) 192
TseI GCWGC 6 cut(s) 17, 278, 457, 479, 513, 516
TspDTI ATGAA 3 cut(s) 384, 486, 507
TspGWI ACGGA 1 cut(s) 131
TspRI CASTG 1 cut(s) 192
VpaK11BI GGWCC 1 cut(s) 103
XspI CTAG 1 cut(s) 664
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.