MD06G1239100.v1.1

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0016850
Physical Location & Seq
Reverse (-)
36965896 .. 36966801
906 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1239100.v1.1.491

Sequence Viewer

Length: 477 bp
ATGGAAAACAAGGCCACAGCTGAGTCCCTACGCATTTACGAAGACTTTGAACCCTTCTGCAAATGGAATGGACGCGACGAGATTGAGCTCCATCTTCGCAGTTTTAGAAAGGAACACCTGAAGGTTCAAATTCATAAGGGATTCGTTATAATCCATGGAGAACGTCCTTTGAAGGAAAGTAATGGAAACACATGGAGGCGCTTCCACAAAGAAATCAAACTTTCAGACAAGTGCAATCCAAATGACATTCACGCTAAGCTTCACAGTGCAGGAGTCCTTACAGTGACAATGCCTGTAGCAGTCAATGCTGGTGATTATGATGCTAATAAACCAACAAGCTGCGAATTTAAGGGCCAAAGGATGTTAGTTTCTAGACGATTGAAAGTGGGAAAAGATGTTACAGTGAAGCTTCTAGCGGCAGTTGCTGTGATTGTAGCTTTTGGATTTGGCGTATATGTTGTCAAATGTTACAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HSP20

Protein Analysis

159

Amino Acids

18.01

Weight (kDa)

9.41

Isoelectric Point (pI)

34.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 27 - 103 2.4e-11 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016850)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g18340
malus_domestica MD06G1239100.v1.1
prunus_persica Prupe.5G242900_v2.0.a1
pyrus_communis pycom06g21460
rosa_chinensis RchiOBHm_Chr7g0177251
rosa_laevigata RLG00000005551
rosa_multiflora Rmu_sc0005310.1_g000013
rosa_roxburghii Rroxscaffold_3G00276010
rosa_rugosa Rorug06G0404200
rosa_samantha Rh7AG004200 Rh7BG004000 Rh7CG003900 Rh7DG003900
rosa_wichuraiana Rw7G000340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 149
AccII CGCG 1 cut(s) 75
AciI CCGC 1 cut(s) 416
AcsI RAATTY 2 cut(s) 129, 344
AcuI CTGAAG 1 cut(s) 140
AgsI TTSAA 4 cut(s) 50, 128, 172, 382
AluBI AGCT 6 cut(s) 20, 88, 259, 339, 409, 437
AluI AGCT 6 cut(s) 20, 88, 259, 339, 409, 437
Alw21I GWGCWC 1 cut(s) 90
AlwNI CAGNNNCTG 1 cut(s) 425
AoxI GGCC 2 cut(s) 12, 352
ApeKI GCWGC 1 cut(s) 339
ApoI RAATTY 2 cut(s) 129, 344
AspLEI GCGC 1 cut(s) 201
AspS9I GGNCC 1 cut(s) 352
AsuHPI GGTGA 1 cut(s) 323
BanII GRGCYC 1 cut(s) 90
BbsI GAAGAC 1 cut(s) 48
Bbv12I GWGCWC 1 cut(s) 90
BbvI GCAGC 1 cut(s) 326
BccI CCATC 1 cut(s) 99
BfaI CTAG 2 cut(s) 372, 413
BfmI CTRYAG 1 cut(s) 294
BfoI RGCGCY 1 cut(s) 202
BisI GCNGC 2 cut(s) 340, 417
BlpI GCTNAGC 1 cut(s) 255
BlsI GCNGC 2 cut(s) 341, 418
BmgT120I GGNCC 1 cut(s) 352
BmsI GCATC 1 cut(s) 310
BpiI GAAGAC 1 cut(s) 48
Bpu1102I GCTNAGC 1 cut(s) 255
BsaJI CCNNGG 1 cut(s) 154
BseDI CCNNGG 1 cut(s) 154
BseGI GGATG 1 cut(s) 366
BseMII CTCAG 1 cut(s) 12
BseXI GCAGC 1 cut(s) 326
BsgI GTGCAG 1 cut(s) 288
Bsh1236I CGCG 1 cut(s) 75
BshFI GGCC 2 cut(s) 14, 354
BsiHKAI GWGCWC 1 cut(s) 90
BslFI GGGAC 1 cut(s) 10
BsmFI GGGAC 1 cut(s) 10
BsnI GGCC 2 cut(s) 14, 354
Bsp1286I GDGCHC 1 cut(s) 90
Bsp1720I GCTNAGC 1 cut(s) 255
Bsp19I CCATGG 1 cut(s) 154
BspACI CCGC 1 cut(s) 416
BspANI GGCC 2 cut(s) 14, 354
BspCNI CTCAG 1 cut(s) 13
BspFNI CGCG 1 cut(s) 75
BssECI CCNNGG 1 cut(s) 154
BssT1I CCWWGG 1 cut(s) 154
Bst4CI ACNGT 3 cut(s) 266, 283, 403
BstAPI GCANNNNNTGC 1 cut(s) 305
BstDEI CTNAG 2 cut(s) 21, 255
BstDSI CCRYGG 1 cut(s) 154
BstF5I GGATG 1 cut(s) 366
BstFNI CGCG 1 cut(s) 75
BstH2I RGCGCY 1 cut(s) 202
BstHHI GCGC 1 cut(s) 201
BstMWI GCNNNNNNNGC 2 cut(s) 305, 422
BstSFI CTRYAG 1 cut(s) 294
BstUI CGCG 1 cut(s) 75
BstV1I GCAGC 1 cut(s) 326
BstV2I GAAGAC 1 cut(s) 48
BsuRI GGCC 2 cut(s) 14, 354
BtgI CCRYGG 1 cut(s) 154
BtsCI GGATG 1 cut(s) 366
BtsIMutI CAGTG 3 cut(s) 271, 288, 408
CaiI CAGNNNCTG 1 cut(s) 425
CfoI GCGC 1 cut(s) 201
Cfr13I GGNCC 1 cut(s) 352
CseI GACGC 1 cut(s) 81
CviAII CATG 2 cut(s) 155, 192
CviJI RGCY 8 cut(s) 14, 20, 88, 259, 339, 354, 409, 437
CviKI_1 RGCY 8 cut(s) 14, 20, 88, 259, 339, 354, 409, 437
DdeI CTNAG 2 cut(s) 21, 255
Ecl136II GAGCTC 1 cut(s) 88
Eco130I CCWWGG 1 cut(s) 154
Eco24I GRGCYC 1 cut(s) 90
Eco53kI GAGCTC 1 cut(s) 88
Eco57I CTGAAG 1 cut(s) 140
EcoICRI GAGCTC 1 cut(s) 88
EcoT14I CCWWGG 1 cut(s) 154
EcoT38I GRGCYC 1 cut(s) 90
ErhI CCWWGG 1 cut(s) 154
FaeI CATG 2 cut(s) 158, 195
FaiI YATR 7 cut(s) 135, 149, 156, 193, 318, 454, 456
FaqI GGGAC 1 cut(s) 10
FatI CATG 2 cut(s) 154, 191
Fnu4HI GCNGC 2 cut(s) 340, 417
FokI GGATG 1 cut(s) 373
FriOI GRGCYC 1 cut(s) 90
Fsp4HI GCNGC 2 cut(s) 340, 417
FspBI CTAG 2 cut(s) 372, 413
GlaI GCGC 1 cut(s) 200
GluI GCNGC 2 cut(s) 340, 417
HaeII RGCGCY 1 cut(s) 202
HaeIII GGCC 2 cut(s) 14, 354
HgaI GACGC 1 cut(s) 81
HhaI GCGC 1 cut(s) 201
Hin1II CATG 2 cut(s) 158, 195
Hin6I GCGC 1 cut(s) 199
HinP1I GCGC 1 cut(s) 199
HindIII AAGCTT 2 cut(s) 257, 407
HinfI GANTC 3 cut(s) 23, 141, 273
HphI GGTGA 1 cut(s) 323
Hpy188I TCNGA 1 cut(s) 226
Hpy188III TCNNGA 1 cut(s) 372
Hpy99I CGWCG 1 cut(s) 80
HpyAV CCTTC 3 cut(s) 64, 115, 166
HpyCH4III ACNGT 3 cut(s) 266, 283, 403
HpyCH4IV ACGT 1 cut(s) 163
HpyCH4V TGCA 3 cut(s) 60, 234, 269
HpyF10VI GCNNNNNNNGC 2 cut(s) 305, 422
HpyF3I CTNAG 2 cut(s) 21, 255
HpySE526I ACGT 1 cut(s) 163
Hsp92II CATG 2 cut(s) 158, 195
HspAI GCGC 1 cut(s) 199
LmnI GCTCC 1 cut(s) 93
LpnPI CCDG 4 cut(s) 131, 255, 294, 306
Lsp1109I GCAGC 1 cut(s) 326
LweI GCATC 1 cut(s) 310
MaeI CTAG 2 cut(s) 372, 413
MaeII ACGT 1 cut(s) 163
MaeIII GTNAC 3 cut(s) 283, 397, 467
MboII GAAGA 2 cut(s) 53, 86
MhlI GDGCHC 1 cut(s) 90
MluCI AATT 3 cut(s) 129, 344, 472
MlyI GAGTC 2 cut(s) 32, 282
MnlI CCTC 1 cut(s) 189
MseI TTAA 2 cut(s) 348, 475
MspA1I CMGCKG 1 cut(s) 20
MvnI CGCG 1 cut(s) 75
MwoI GCNNNNNNNGC 2 cut(s) 305, 422
NcoI CCATGG 1 cut(s) 154
NlaIII CATG 2 cut(s) 158, 195
NmuCI GTSAC 1 cut(s) 283
PfeI GAWTC 1 cut(s) 141
PkrI GCNGC 2 cut(s) 341, 418
PleI GAGTC 2 cut(s) 31, 281
PpsI GAGTC 2 cut(s) 31, 281
PsiI TTATAA 1 cut(s) 149
Psp124BI GAGCTC 1 cut(s) 90
PspPI GGNCC 1 cut(s) 352
PstNI CAGNNNCTG 1 cut(s) 425
PvuII CAGCTG 1 cut(s) 20
SacI GAGCTC 1 cut(s) 90
SaqAI TTAA 2 cut(s) 348, 475
SatI GCNGC 2 cut(s) 340, 417
Sau96I GGNCC 1 cut(s) 352
SchI GAGTC 2 cut(s) 32, 282
SduI GDGCHC 1 cut(s) 90
SetI ASST 9 cut(s) 22, 90, 120, 126, 166, 261, 341, 411, 439
SfaNI GCATC 1 cut(s) 310
SfcI CTRYAG 1 cut(s) 294
Sse9I AATT 3 cut(s) 129, 344, 472
SsiI CCGC 1 cut(s) 416
SspMI CTAG 2 cut(s) 372, 413
SstI GAGCTC 1 cut(s) 90
StyI CCWWGG 1 cut(s) 154
TaaI ACNGT 3 cut(s) 266, 283, 403
TaiI ACGT 1 cut(s) 166
TasI AATT 3 cut(s) 129, 344, 472
TauI GCSGC 1 cut(s) 419
TfiI GAWTC 1 cut(s) 141
Tru1I TTAA 2 cut(s) 348, 475
Tru9I TTAA 2 cut(s) 348, 475
TscAI CASTG 3 cut(s) 271, 288, 408
TseFI GTSAC 1 cut(s) 283
TseI GCWGC 1 cut(s) 339
Tsp45I GTSAC 1 cut(s) 283
TspDTI ATGAA 1 cut(s) 122
TspRI CASTG 3 cut(s) 271, 288, 408
XapI RAATTY 2 cut(s) 129, 344
XbaI TCTAGA 1 cut(s) 371
XspI CTAG 2 cut(s) 372, 413
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.