Rh7CG003900

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0016850
Physical Location & Seq
Forward (+)
354615 .. 355379
765 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG003900.1

Sequence Viewer

Length: 660 bp
ATGGATCAAAGCAAGGCAGCCGGAGCTGATCATCAGGCCAAACGAACTTACGAGGACTTTGAACCCTTCTGCAAATGGCATAACAATGTTGTTGAGGTCCATATTCAAGGTTTCAGAAAGGAACACCTTAGTGTCCGTACCACTCAGACGAGGATGCTTACAATCCATGGAGAACGTCCATTGGACCAAACTAAATCTACATGGACCCGTTTCCACAAAGAAATTAATCTTCCAAACAACTGCGACACAAATAGAGTTGCGGCAAAGTTTGCTGCGGGAGTTCTTACAATAACAATGCCTCCCAAAATTGCACCACCTCAACCTCAATCTCCAGAAGATCAGAAACAACAGCTTAGCAATCATGAAAAGTTACCAGAACCAAGCAACAATGATACTCGAACAAATAATGATAAAGCGATAGCTAAACTCATTAAACAAAATCCGGACAAGCAGCCAACCACCGATGAAAATGCTGCATTGCTTGATGAAGAGGTTAAGCCAGGAAGCTGCTGCGAATTGGGGCAAAAGAGTACTTTAGCTTCCAAGAATTGGAAGGTGAAACTCGTGTTGGTGGTTGCCGTGGTGGTCGCTTTTGGTTTCGGGGCATATATCAGATACAAACACGGCCATTACTCATACAGCTGGGAGCAGACAGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HSP20

Protein Analysis

219

Amino Acids

24.83

Weight (kDa)

8.59

Isoelectric Point (pI)

39.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 30 - 107 3.5e-12 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016850)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g18340
malus_domestica MD06G1239100.v1.1
prunus_persica Prupe.5G242900_v2.0.a1
pyrus_communis pycom06g21460
rosa_chinensis RchiOBHm_Chr7g0177251
rosa_laevigata RLG00000005551
rosa_multiflora Rmu_sc0005310.1_g000013
rosa_roxburghii Rroxscaffold_3G00276010
rosa_rugosa Rorug06G0404200
rosa_samantha Rh7AG004200 Rh7BG004000 Rh7CG003900 Rh7DG003900
rosa_wichuraiana Rw7G000340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 549
AccIII TCCGGA 1 cut(s) 442
AciI CCGC 2 cut(s) 260, 275
AclWI GGATC 1 cut(s) 12
AcoI YGGCCR 1 cut(s) 625
AfaI GTAC 2 cut(s) 139, 532
AfiI CCNNNNNNNGG 1 cut(s) 549
AgsI TTSAA 2 cut(s) 62, 107
AjnI CCWGG 1 cut(s) 499
AjuI GAANNNNNNNTTGG 2 cut(s) 551, 583
AleI CACNNNNGTG 1 cut(s) 129
AluBI AGCT 6 cut(s) 26, 352, 422, 507, 539, 642
AluI AGCT 6 cut(s) 26, 352, 422, 507, 539, 642
AlwI GGATC 1 cut(s) 12
Aor13HI TCCGGA 1 cut(s) 442
AoxI GGCC 2 cut(s) 36, 625
ApeKI GCWGC 6 cut(s) 17, 272, 451, 473, 507, 510
AseI ATTAAT 1 cut(s) 225
AspS9I GGNCC 3 cut(s) 97, 184, 204
AsuHPI GGTGA 1 cut(s) 568
AvaII GGWCC 3 cut(s) 97, 184, 204
BarI GAAGNNNNNNTAC 2 cut(s) 523, 555
BauI CACGAG 1 cut(s) 563
BbvI GCAGC 6 cut(s) 29, 259, 460, 463, 494, 497
BceAI ACGGC 2 cut(s) 563, 640
BcgI CGANNNNNNTGC 2 cut(s) 452, 486
BciT130I CCWGG 1 cut(s) 501
BclI TGATCA 1 cut(s) 28
BfaI CTAG 1 cut(s) 658
BisI GCNGC 7 cut(s) 18, 261, 273, 452, 474, 508, 511
BlpI GCTNAGC 1 cut(s) 353
BlsI GCNGC 7 cut(s) 19, 262, 274, 453, 475, 509, 512
BmcAI AGTACT 1 cut(s) 532
Bme1390I CCNGG 1 cut(s) 501
Bme18I GGWCC 3 cut(s) 97, 184, 204
BmgT120I GGNCC 3 cut(s) 97, 184, 204
BmiI GGNNCC 1 cut(s) 206
BmrFI CCNGG 1 cut(s) 501
BmsI GCATC 1 cut(s) 144
BpmI CTGGAG 1 cut(s) 315
Bpu1102I GCTNAGC 1 cut(s) 353
BsaJI CCNNGG 2 cut(s) 166, 579
BsaWI WCCGGW 1 cut(s) 442
BsaXI ACNNNNNCTCC 2 cut(s) 283, 313
Bsc4I CCNNNNNNNGG 1 cut(s) 549
Bse3DI GCAATG 1 cut(s) 476
BseAI TCCGGA 1 cut(s) 442
BseBI CCWGG 1 cut(s) 501
BseDI CCNNGG 2 cut(s) 166, 579
BseGI GGATG 1 cut(s) 159
BseLI CCNNNNNNNGG 1 cut(s) 549
BseMI GCAATG 1 cut(s) 476
BseMII CTCAG 1 cut(s) 158
BseXI GCAGC 6 cut(s) 29, 259, 460, 463, 494, 497
BseYI CCCAGC 1 cut(s) 642
BshFI GGCC 2 cut(s) 38, 627
BsiSI CCGG 2 cut(s) 21, 443
BslI CCNNNNNNNGG 1 cut(s) 549
BsnI GGCC 2 cut(s) 38, 627
Bsp13I TCCGGA 1 cut(s) 442
Bsp143I GATC 3 cut(s) 4, 28, 337
Bsp1720I GCTNAGC 1 cut(s) 353
Bsp19I CCATGG 1 cut(s) 166
BspACI CCGC 2 cut(s) 260, 275
BspANI GGCC 2 cut(s) 38, 627
BspCNI CTCAG 1 cut(s) 157
BspEI TCCGGA 1 cut(s) 442
BspHI TCATGA 1 cut(s) 361
BspLI GGNNCC 1 cut(s) 206
BspPI GGATC 1 cut(s) 12
BsrDI GCAATG 1 cut(s) 476
BssECI CCNNGG 2 cut(s) 166, 579
BssMI GATC 3 cut(s) 4, 28, 337
BssSI CACGAG 1 cut(s) 563
BssT1I CCWWGG 1 cut(s) 166
Bst2BI CACGAG 1 cut(s) 563
Bst2UI CCWGG 1 cut(s) 501
Bst6I CTCTTC 1 cut(s) 483
BstAPI GCANNNNNTGC 1 cut(s) 269
BstDEI CTNAG 3 cut(s) 128, 144, 353
BstDSI CCRYGG 2 cut(s) 166, 579
BstF5I GGATG 1 cut(s) 159
BstKTI GATC 3 cut(s) 7, 31, 340
BstMBI GATC 3 cut(s) 4, 28, 337
BstMWI GCNNNNNNNGC 2 cut(s) 23, 269
BstNI CCWGG 1 cut(s) 501
BstSCI CCNGG 1 cut(s) 499
BstV1I GCAGC 6 cut(s) 29, 259, 460, 463, 494, 497
BsuRI GGCC 2 cut(s) 38, 627
BtgI CCRYGG 2 cut(s) 166, 579
BtsCI GGATG 1 cut(s) 159
CciI TCATGA 1 cut(s) 361
Cfr13I GGNCC 3 cut(s) 97, 184, 204
Csp6I GTAC 2 cut(s) 138, 531
CviAII CATG 3 cut(s) 167, 201, 362
CviQI GTAC 2 cut(s) 138, 531
DdeI CTNAG 3 cut(s) 128, 144, 353
DpnI GATC 3 cut(s) 6, 30, 339
DpnII GATC 3 cut(s) 4, 28, 337
EaeI YGGCCR 1 cut(s) 625
Eam1104I CTCTTC 1 cut(s) 483
EarI CTCTTC 1 cut(s) 483
Eco130I CCWWGG 1 cut(s) 166
Eco47I GGWCC 3 cut(s) 97, 184, 204
EcoRII CCWGG 1 cut(s) 499
EcoT14I CCWWGG 1 cut(s) 166
ErhI CCWWGG 1 cut(s) 166
FaeI CATG 3 cut(s) 170, 204, 365
FaiI YATR 8 cut(s) 81, 102, 168, 202, 363, 607, 609, 637
FatI CATG 3 cut(s) 166, 200, 361
FauI CCCGC 1 cut(s) 268
FbaI TGATCA 1 cut(s) 28
Fnu4HI GCNGC 7 cut(s) 18, 261, 273, 452, 474, 508, 511
FokI GGATG 1 cut(s) 166
Fsp4HI GCNGC 7 cut(s) 18, 261, 273, 452, 474, 508, 511
FspBI CTAG 1 cut(s) 658
GluI GCNGC 7 cut(s) 18, 261, 273, 452, 474, 508, 511
GsaI CCCAGC 1 cut(s) 646
GsuI CTGGAG 1 cut(s) 315
HaeIII GGCC 2 cut(s) 38, 627
HapII CCGG 2 cut(s) 21, 443
Hin1II CATG 3 cut(s) 170, 204, 365
HpaII CCGG 2 cut(s) 21, 443
HphI GGTGA 1 cut(s) 568
Hpy188I TCNGA 4 cut(s) 116, 147, 342, 614
Hpy188III TCNNGA 3 cut(s) 332, 362, 443
HpyAV CCTTC 2 cut(s) 76, 547
HpyCH4IV ACGT 1 cut(s) 175
HpyCH4V TGCA 3 cut(s) 72, 311, 476
HpyF10VI GCNNNNNNNGC 2 cut(s) 23, 269
HpyF3I CTNAG 3 cut(s) 128, 144, 353
HpySE526I ACGT 1 cut(s) 175
Hsp92II CATG 3 cut(s) 170, 204, 365
Kpn2I TCCGGA 1 cut(s) 442
Ksp22I TGATCA 1 cut(s) 28
Kzo9I GATC 3 cut(s) 4, 28, 337
LmnI GCTCC 2 cut(s) 23, 646
LpnPI CCDG 8 cut(s) 20, 34, 345, 387, 456, 486, 513, 628
Lsp1109I GCAGC 6 cut(s) 29, 259, 460, 463, 494, 497
LweI GCATC 1 cut(s) 144
MaeI CTAG 1 cut(s) 658
MaeII ACGT 1 cut(s) 175
MaeIII GTNAC 1 cut(s) 369
MalI GATC 3 cut(s) 6, 30, 339
MboI GATC 3 cut(s) 4, 28, 337
MboII GAAGA 3 cut(s) 221, 347, 500
MluCI AATT 4 cut(s) 222, 306, 515, 547
MnlI CCTC 7 cut(s) 46, 88, 144, 309, 327, 333, 484
MroI TCCGGA 1 cut(s) 442
MseI TTAA 3 cut(s) 225, 432, 495
MslI CAYNNNNRTG 2 cut(s) 84, 129
MspA1I CMGCKG 1 cut(s) 642
MspI CCGG 2 cut(s) 21, 443
MspR9I CCNGG 1 cut(s) 501
MvaI CCWGG 1 cut(s) 501
MwoI GCNNNNNNNGC 2 cut(s) 23, 269
NcoI CCATGG 1 cut(s) 166
NdeII GATC 3 cut(s) 4, 28, 337
NlaIII CATG 3 cut(s) 170, 204, 365
NlaIV GGNNCC 1 cut(s) 206
OliI CACNNNNGTG 1 cut(s) 129
PagI TCATGA 1 cut(s) 361
PflMI CCANNNNNTGG 1 cut(s) 549
PkrI GCNGC 7 cut(s) 19, 262, 274, 453, 475, 509, 512
PshBI ATTAAT 1 cut(s) 225
Psp6I CCWGG 1 cut(s) 499
PspFI CCCAGC 1 cut(s) 642
PspGI CCWGG 1 cut(s) 499
PspN4I GGNNCC 1 cut(s) 206
PspPI GGNCC 3 cut(s) 97, 184, 204
PvuII CAGCTG 1 cut(s) 642
RsaI GTAC 2 cut(s) 139, 532
RsaNI GTAC 2 cut(s) 138, 531
RseI CAYNNNNRTG 2 cut(s) 84, 129
SaqAI TTAA 3 cut(s) 225, 432, 495
SatI GCNGC 7 cut(s) 18, 261, 273, 452, 474, 508, 511
Sau3AI GATC 3 cut(s) 4, 28, 337
Sau96I GGNCC 3 cut(s) 97, 184, 204
ScaI AGTACT 1 cut(s) 532
ScrFI CCNGG 1 cut(s) 501
SfaNI GCATC 1 cut(s) 144
SinI GGWCC 3 cut(s) 97, 184, 204
SmiMI CAYNNNNRTG 2 cut(s) 84, 129
Sse9I AATT 4 cut(s) 222, 306, 515, 547
SsiI CCGC 2 cut(s) 260, 275
SspMI CTAG 1 cut(s) 658
StyD4I CCNGG 1 cut(s) 499
StyI CCWWGG 1 cut(s) 166
TaiI ACGT 1 cut(s) 178
TaqI TCGA 1 cut(s) 397
TasI AATT 4 cut(s) 222, 306, 515, 547
TatI WGTACW 1 cut(s) 530
TauI GCSGC 1 cut(s) 263
Tru1I TTAA 3 cut(s) 225, 432, 495
Tru9I TTAA 3 cut(s) 225, 432, 495
TseI GCWGC 6 cut(s) 17, 272, 451, 473, 507, 510
TspDTI ATGAA 3 cut(s) 378, 480, 501
TspGWI ACGGA 1 cut(s) 125
Van91I CCANNNNNTGG 1 cut(s) 549
VpaK11BI GGWCC 3 cut(s) 97, 184, 204
VspI ATTAAT 1 cut(s) 225
XspI CTAG 1 cut(s) 658
ZrmI AGTACT 1 cut(s) 532
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.