Rroxscaffold_3G00276010

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0016850
Physical Location & Seq
Reverse (-)
67301363 .. 67302308
946 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00276010.1

Sequence Viewer

Length: 603 bp
ATGGATCAAAGCAAGGCAGCCGGAGCTGATCATCAGGCCAAACGTACTTACGAGGACTTTGAACCCTTCTGCAAATGGCATAACCGCGACAATGTTGTTGAGGTCCATATTCAAGGTTTCAGAAAGGAAAACCTAAGTGTCCGTACCACTCAGACGGGGATGCTTACAATCCATGGAGAACGTCCATTGGACCAAACTAAATCTACATGGAGCCGTTTCCACAAAGAAATTAAGCTTCCAAACAACTGCGACACAAATAGAGTTGCTGCAAAGTTTGCTGCAGGAGTTCTTACAATAACAATGCCTCCCAAAGTTGCATCACCTCAACCTCAAACAAATAATGATAAAGCGATAGCTAAACTCATTAAACAAAATCCGGACAAGCAGCCAACCACCGATGAAAATGCTGCCTTGCTTGATGAAGAGGTTAAGCCAGGAAGCTGCTGCGAATTCGGGCAAAAGAGTACTTTAGCTTCCAAGAATTGGAAGGTGAAACTCGTGTTGGTGGTTGCCGTGGTGGTCGCTTTTGGTTTCGGGGCATATATCAGATACAAACACGGCCATTACGCTCATATAGCTGGGAGCAGACAGACTAGATCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HSP20

Protein Analysis

200

Amino Acids

22.39

Weight (kDa)

9.45

Isoelectric Point (pI)

38.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 32 - 109 3.8e-13 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016850)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g18340
malus_domestica MD06G1239100.v1.1
prunus_persica Prupe.5G242900_v2.0.a1
pyrus_communis pycom06g21460
rosa_chinensis RchiOBHm_Chr7g0177251
rosa_laevigata RLG00000005551
rosa_multiflora Rmu_sc0005310.1_g000013
rosa_roxburghii Rroxscaffold_3G00276010
rosa_rugosa Rorug06G0404200
rosa_samantha Rh7AG004200 Rh7BG004000 Rh7CG003900 Rh7DG003900
rosa_wichuraiana Rw7G000340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 483
AccII CGCG 1 cut(s) 87
AccIII TCCGGA 1 cut(s) 376
AciI CCGC 1 cut(s) 85
AclWI GGATC 1 cut(s) 12
AcoI YGGCCR 1 cut(s) 559
AcsI RAATTY 1 cut(s) 449
AfaI GTAC 3 cut(s) 46, 145, 466
AfiI CCNNNNNNNGG 1 cut(s) 483
AgsI TTSAA 2 cut(s) 62, 113
AjnI CCWGG 1 cut(s) 433
AjuI GAANNNNNNNTTGG 2 cut(s) 485, 517
AluBI AGCT 6 cut(s) 26, 235, 356, 441, 473, 578
AluI AGCT 6 cut(s) 26, 235, 356, 441, 473, 578
AlwI GGATC 1 cut(s) 12
Aor13HI TCCGGA 1 cut(s) 376
AoxI GGCC 2 cut(s) 36, 559
ApeKI GCWGC 7 cut(s) 17, 266, 278, 385, 407, 441, 444
ApoI RAATTY 1 cut(s) 449
AspS9I GGNCC 2 cut(s) 103, 190
AsuHPI GGTGA 2 cut(s) 312, 502
AvaII GGWCC 2 cut(s) 103, 190
BarI GAAGNNNNNNTAC 2 cut(s) 457, 489
BauI CACGAG 1 cut(s) 497
BbvI GCAGC 7 cut(s) 29, 253, 265, 394, 397, 428, 431
BceAI ACGGC 3 cut(s) 198, 497, 574
BcgI CGANNNNNNTGC 2 cut(s) 386, 420
BciT130I CCWGG 1 cut(s) 435
BclI TGATCA 1 cut(s) 28
BfaI CTAG 1 cut(s) 594
BfmI CTRYAG 1 cut(s) 279
BglII AGATCT 1 cut(s) 596
BisI GCNGC 7 cut(s) 18, 267, 279, 386, 408, 442, 445
BlsI GCNGC 7 cut(s) 19, 268, 280, 387, 409, 443, 446
BmcAI AGTACT 1 cut(s) 466
Bme1390I CCNGG 1 cut(s) 435
Bme18I GGWCC 2 cut(s) 103, 190
BmgT120I GGNCC 2 cut(s) 103, 190
BmiI GGNNCC 1 cut(s) 212
BmrFI CCNGG 1 cut(s) 435
BmsI GCATC 2 cut(s) 150, 326
BsaJI CCNNGG 2 cut(s) 172, 513
BsaWI WCCGGW 1 cut(s) 376
BsaXI ACNNNNNCTCC 2 cut(s) 289, 319
Bsc4I CCNNNNNNNGG 1 cut(s) 483
BseAI TCCGGA 1 cut(s) 376
BseBI CCWGG 1 cut(s) 435
BseDI CCNNGG 2 cut(s) 172, 513
BseGI GGATG 1 cut(s) 165
BseLI CCNNNNNNNGG 1 cut(s) 483
BseMII CTCAG 1 cut(s) 164
BseXI GCAGC 7 cut(s) 29, 253, 265, 394, 397, 428, 431
BseYI CCCAGC 1 cut(s) 578
Bsh1236I CGCG 1 cut(s) 87
BshFI GGCC 2 cut(s) 38, 561
BsiSI CCGG 2 cut(s) 21, 377
BslI CCNNNNNNNGG 1 cut(s) 483
BsnI GGCC 2 cut(s) 38, 561
Bsp13I TCCGGA 1 cut(s) 376
Bsp143I GATC 3 cut(s) 4, 28, 596
Bsp19I CCATGG 1 cut(s) 172
BspACI CCGC 1 cut(s) 85
BspANI GGCC 2 cut(s) 38, 561
BspCNI CTCAG 1 cut(s) 163
BspEI TCCGGA 1 cut(s) 376
BspFNI CGCG 1 cut(s) 87
BspLI GGNNCC 1 cut(s) 212
BspMAI CTGCAG 1 cut(s) 283
BspPI GGATC 1 cut(s) 12
BssECI CCNNGG 2 cut(s) 172, 513
BssMI GATC 3 cut(s) 4, 28, 596
BssSI CACGAG 1 cut(s) 497
BssT1I CCWWGG 1 cut(s) 172
Bst2BI CACGAG 1 cut(s) 497
Bst2UI CCWGG 1 cut(s) 435
Bst6I CTCTTC 1 cut(s) 417
BstAPI GCANNNNNTGC 1 cut(s) 275
BstDEI CTNAG 2 cut(s) 134, 150
BstDSI CCRYGG 2 cut(s) 172, 513
BstF5I GGATG 1 cut(s) 165
BstFNI CGCG 1 cut(s) 87
BstKTI GATC 3 cut(s) 7, 31, 599
BstMBI GATC 3 cut(s) 4, 28, 596
BstMWI GCNNNNNNNGC 3 cut(s) 23, 275, 575
BstNI CCWGG 1 cut(s) 435
BstSCI CCNGG 1 cut(s) 433
BstSFI CTRYAG 1 cut(s) 279
BstUI CGCG 1 cut(s) 87
BstV1I GCAGC 7 cut(s) 29, 253, 265, 394, 397, 428, 431
BstX2I RGATCY 1 cut(s) 596
BstYI RGATCY 1 cut(s) 596
BsuRI GGCC 2 cut(s) 38, 561
BtgI CCRYGG 2 cut(s) 172, 513
BtsCI GGATG 1 cut(s) 165
Cfr13I GGNCC 2 cut(s) 103, 190
Csp6I GTAC 3 cut(s) 45, 144, 465
CviAII CATG 2 cut(s) 173, 207
CviQI GTAC 3 cut(s) 45, 144, 465
DdeI CTNAG 2 cut(s) 134, 150
DpnI GATC 3 cut(s) 6, 30, 598
DpnII GATC 3 cut(s) 4, 28, 596
EaeI YGGCCR 1 cut(s) 559
Eam1104I CTCTTC 1 cut(s) 417
EarI CTCTTC 1 cut(s) 417
Eco130I CCWWGG 1 cut(s) 172
Eco47I GGWCC 2 cut(s) 103, 190
EcoRI GAATTC 1 cut(s) 449
EcoRII CCWGG 1 cut(s) 433
EcoT14I CCWWGG 1 cut(s) 172
ErhI CCWWGG 1 cut(s) 172
FaeI CATG 2 cut(s) 176, 210
FaiI YATR 8 cut(s) 81, 108, 174, 208, 541, 543, 573, 575
FatI CATG 2 cut(s) 172, 206
FbaI TGATCA 1 cut(s) 28
Fnu4HI GCNGC 7 cut(s) 18, 267, 279, 386, 408, 442, 445
FokI GGATG 1 cut(s) 172
Fsp4HI GCNGC 7 cut(s) 18, 267, 279, 386, 408, 442, 445
FspBI CTAG 1 cut(s) 594
GluI GCNGC 7 cut(s) 18, 267, 279, 386, 408, 442, 445
GsaI CCCAGC 1 cut(s) 582
HaeIII GGCC 2 cut(s) 38, 561
HapII CCGG 2 cut(s) 21, 377
Hin1II CATG 2 cut(s) 176, 210
HindIII AAGCTT 1 cut(s) 233
HpaII CCGG 2 cut(s) 21, 377
HphI GGTGA 2 cut(s) 312, 502
Hpy188I TCNGA 3 cut(s) 122, 153, 548
Hpy188III TCNNGA 1 cut(s) 377
HpyAV CCTTC 2 cut(s) 76, 481
HpyCH4IV ACGT 2 cut(s) 43, 181
HpyCH4V TGCA 4 cut(s) 72, 269, 281, 317
HpyF10VI GCNNNNNNNGC 3 cut(s) 23, 275, 575
HpyF3I CTNAG 2 cut(s) 134, 150
HpySE526I ACGT 2 cut(s) 43, 181
Hsp92II CATG 2 cut(s) 176, 210
Kpn2I TCCGGA 1 cut(s) 376
Ksp22I TGATCA 1 cut(s) 28
Kzo9I GATC 3 cut(s) 4, 28, 596
LmnI GCTCC 3 cut(s) 23, 210, 582
LpnPI CCDG 7 cut(s) 20, 34, 267, 390, 420, 447, 564
Lsp1109I GCAGC 7 cut(s) 29, 253, 265, 394, 397, 428, 431
LweI GCATC 2 cut(s) 150, 326
MaeI CTAG 1 cut(s) 594
MaeII ACGT 2 cut(s) 43, 181
MalI GATC 3 cut(s) 6, 30, 598
MboI GATC 3 cut(s) 4, 28, 596
MboII GAAGA 1 cut(s) 434
MflI RGATCY 1 cut(s) 596
MluCI AATT 3 cut(s) 228, 449, 481
MnlI CCTC 6 cut(s) 46, 94, 315, 333, 339, 418
MroI TCCGGA 1 cut(s) 376
MseI TTAA 4 cut(s) 231, 366, 429, 601
MspI CCGG 2 cut(s) 21, 377
MspR9I CCNGG 1 cut(s) 435
MvaI CCWGG 1 cut(s) 435
MvnI CGCG 1 cut(s) 87
MwoI GCNNNNNNNGC 3 cut(s) 23, 275, 575
NcoI CCATGG 1 cut(s) 172
NdeII GATC 3 cut(s) 4, 28, 596
NlaIII CATG 2 cut(s) 176, 210
NlaIV GGNNCC 1 cut(s) 212
PflMI CCANNNNNTGG 1 cut(s) 483
PkrI GCNGC 7 cut(s) 19, 268, 280, 387, 409, 443, 446
Psp6I CCWGG 1 cut(s) 433
PspFI CCCAGC 1 cut(s) 578
PspGI CCWGG 1 cut(s) 433
PspN4I GGNNCC 1 cut(s) 212
PspPI GGNCC 2 cut(s) 103, 190
PstI CTGCAG 1 cut(s) 283
PsuI RGATCY 1 cut(s) 596
RsaI GTAC 3 cut(s) 46, 145, 466
RsaNI GTAC 3 cut(s) 45, 144, 465
SaqAI TTAA 4 cut(s) 231, 366, 429, 601
SatI GCNGC 7 cut(s) 18, 267, 279, 386, 408, 442, 445
Sau3AI GATC 3 cut(s) 4, 28, 596
Sau96I GGNCC 2 cut(s) 103, 190
ScaI AGTACT 1 cut(s) 466
ScrFI CCNGG 1 cut(s) 435
SfaNI GCATC 2 cut(s) 150, 326
SfcI CTRYAG 1 cut(s) 279
SinI GGWCC 2 cut(s) 103, 190
Sse9I AATT 3 cut(s) 228, 449, 481
SsiI CCGC 1 cut(s) 85
SspMI CTAG 1 cut(s) 594
StyD4I CCNGG 1 cut(s) 433
StyI CCWWGG 1 cut(s) 172
TaiI ACGT 2 cut(s) 46, 184
TasI AATT 3 cut(s) 228, 449, 481
TatI WGTACW 1 cut(s) 464
Tru1I TTAA 4 cut(s) 231, 366, 429, 601
Tru9I TTAA 4 cut(s) 231, 366, 429, 601
TseI GCWGC 7 cut(s) 17, 266, 278, 385, 407, 441, 444
TspDTI ATGAA 2 cut(s) 414, 435
TspGWI ACGGA 1 cut(s) 131
Van91I CCANNNNNTGG 1 cut(s) 483
VpaK11BI GGWCC 2 cut(s) 103, 190
XapI RAATTY 1 cut(s) 449
XspI CTAG 1 cut(s) 594
ZrmI AGTACT 1 cut(s) 466
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.