FvH4_5g18700

HIT zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0010570
Physical Location & Seq
Forward (+)
10749894 .. 10755594
5701 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g18700.t5

Sequence Viewer

Length: 1320 bp
ATGACGACGAGGACGAATTTCTACAAGAACCCTTCCATTACTTACAAGAAGGACCTCTCTCTCTCCTCCGTTCTTCAGAATCTCAAAGCTTACAACACCGCCACCGGCAATGCTCCGCCGCCTGAAGAGCAGACGCCCACCTCCGATGCCAAAACGACAGGTCGTAAACGCCAGCGCGATCCAAAACCACCGCCGCGTGGCGGGAAGCGTGAAATTGAAGAGCGCGATGGACCTATGTCGCATCAGGATTACATCGACAAGAGAAGAAAAGAAGCTTTTGCAAAGCCAGCTTTTGAAGAATTGACTGCGGATGTTTTGGGAAAGCAGGGACCTTCTGGTTCGTGTTTAAATTTGGTACAGTATGACAGTGATGAGAGTAGTTCTTCGGAATGTGAAGAGAAGCAGGATCCTCCAAATTCTGGTCAGGCGAATGAATCTGATGGAGTAAAGAGCAGAAGTGAACAGCGGTACCCTCACCCAGGCGAACCTGTTTGTGTGATGTGCGGTAAATATGGAGAATATATATGCAACGAGACCGATGATGACATTTGCAGCATGGATTGCAAAGCTGAATTACTGGAATCTCTTAAAGCTGTCAAGGATCCATTAAGTAATGAAAGACCTGATGTCTCCTCATCCGGGCCCAGCTATACCTTACCGATGCCCGATTTTGGTGAAGACACGTGGGATTATGAACGTCATCGCTGGTCGAAGAAGACTTCCAATCTTTGTACATATGAATGTTGGAAATGCAAGAGGCCTGGACACCTTGCTCAAGATTGTTTGGTGATGACACGAAACCAGATGACATTGGGCCAAAGCAATTCAATACCAGCGGACCTTGTTGCCTTATACAGAAGATGCCACCAGATAGGGAAAAACATGTCAGTGGCAAAGTGCAATGAATGCTACAGCTCATTAAGTTTAGCTACATGCCTTGATTGTAGTACTGCCCTCTGTGACAATGCAGGTCATTTGCATGAGCATATACAGAGACATCCATCCCATCGACAATATTATTCTCATAAACTCTCCCGTCTGGTCAAATGCTGTAAATCAACATGCAAGGTGACTGACATTAAGGATCTTTTGGCATGCCAATACTGTTTTGATAAAGCTTTTGATAAGTTCTATGACATGTATACTGCAACCTGGAAAGGAACTGGTCTTTCAATTATATCAGGTTCTGTTTGCTGTGAGGATCACTTTGATTGGCATCGTATGAACTGTTTCAATGCTGGTGCGGAAGACAGTGGATATATCATCAGTAGGACATCCCTGAAAGAGAAGCATATTCAGATTAGTGACTTTATCTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-CCHC zf-HIT

Protein Analysis

440

Amino Acids

49.56

Weight (kDa)

6.4

Isoelectric Point (pI)

52.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-HIT PF04438 174 - 201 4.4e-06 HIT zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 959
Acc65I GGTACC 1 cut(s) 468
AccB1I GGYRCC 1 cut(s) 468
AccB7I CCANNNNNTGG 1 cut(s) 419
AccI GTMKAC 1 cut(s) 1142
AccII CGCG 3 cut(s) 177, 196, 225
AclWI GGATC 7 cut(s) 173, 401, 414, 596, 609, 1092, 1209
AcsI RAATTY 3 cut(s) 16, 349, 415
AcuI CTGAAG 2 cut(s) 59, 144
AcvI CACGTG 1 cut(s) 684
AcyI GRCGYC 1 cut(s) 134
AfaI GTAC 4 cut(s) 357, 470, 733, 949
AfiI CCNNNNNNNGG 6 cut(s) 197, 200, 419, 479, 639, 671
AflIII ACRYGT 3 cut(s) 681, 882, 1137
AgsI TTSAA 5 cut(s) 218, 296, 828, 1173, 1234
AhdI GACNNNNNGTC 1 cut(s) 626
AjnI CCWGG 3 cut(s) 478, 760, 1151
AluBI AGCT 9 cut(s) 89, 275, 290, 569, 593, 648, 915, 929, 1118
AluI AGCT 9 cut(s) 89, 275, 290, 569, 593, 648, 915, 929, 1118
Alw26I GTCTC 3 cut(s) 527, 634, 988
AlwI GGATC 7 cut(s) 173, 401, 414, 596, 609, 1092, 1209
AlwNI CAGNNNCTG 1 cut(s) 1187
AoxI GGCC 3 cut(s) 641, 758, 814
ApaI GGGCCC 1 cut(s) 645
ApeKI GCWGC 1 cut(s) 552
ApoI RAATTY 3 cut(s) 16, 349, 415
Asp700I GAANNNNTTC 1 cut(s) 1229
Asp718I GGTACC 1 cut(s) 468
AspLEI GCGC 2 cut(s) 177, 225
AspS9I GGNCC 7 cut(s) 52, 230, 329, 641, 642, 814, 838
AsuC2I CCSGG 1 cut(s) 640
AsuHPI GGTGA 4 cut(s) 467, 686, 799, 1081
AvaII GGWCC 4 cut(s) 52, 230, 329, 838
BaeGI GKGCMC 1 cut(s) 645
BaeI ACNNNNGTAYC 2 cut(s) 452, 485
BamHI GGATCC 2 cut(s) 406, 601
BanI GGYRCC 1 cut(s) 468
BanII GRGCYC 1 cut(s) 645
BbrPI CACGTG 1 cut(s) 684
BbsI GAAGAC 3 cut(s) 684, 722, 1254
BbvI GCAGC 1 cut(s) 564
BccI CCATC 4 cut(s) 221, 434, 1009, 1014
BciT130I CCWGG 3 cut(s) 480, 762, 1153
BcnI CCSGG 1 cut(s) 640
BcoDI GTCTC 3 cut(s) 527, 634, 988
BfmI CTRYAG 1 cut(s) 910
BfuAI ACCTGC 1 cut(s) 959
BisI GCNGC 3 cut(s) 119, 194, 553
BlsI GCNGC 3 cut(s) 120, 195, 554
BmcAI AGTACT 1 cut(s) 949
Bme1390I CCNGG 4 cut(s) 480, 640, 762, 1153
Bme18I GGWCC 4 cut(s) 52, 230, 329, 838
BmeRI GACNNNNNGTC 1 cut(s) 626
BmgT120I GGNCC 7 cut(s) 52, 230, 329, 641, 642, 814, 838
BmiI GGNNCC 5 cut(s) 330, 408, 470, 603, 643
BmrFI CCNGG 4 cut(s) 480, 640, 762, 1153
BmsI GCATC 5 cut(s) 136, 250, 651, 851, 1225
BoxI GACNNNNGTC 1 cut(s) 235
BpiI GAAGAC 3 cut(s) 684, 722, 1254
BpuEI CTTGAG 1 cut(s) 759
BpuMI CCSGG 1 cut(s) 640
BsaAI YACGTR 1 cut(s) 684
BsaBI GATNNNNATC 1 cut(s) 1215
BsaHI GRCGYC 1 cut(s) 134
BsaI GGTCTC 1 cut(s) 527
BsaJI CCNNGG 1 cut(s) 478
Bsc4I CCNNNNNNNGG 6 cut(s) 197, 200, 419, 479, 639, 671
Bse118I RCCGGY 1 cut(s) 104
Bse1I ACTGG 2 cut(s) 582, 1168
Bse3DI GCAATG 2 cut(s) 115, 907
Bse8I GATNNNNATC 1 cut(s) 1215
BseBI CCWGG 3 cut(s) 480, 762, 1153
BseDI CCNNGG 1 cut(s) 478
BseGI GGATG 5 cut(s) 316, 635, 997, 1001, 1274
BseJI GATNNNNATC 1 cut(s) 1215
BseLI CCNNNNNNNGG 6 cut(s) 197, 200, 419, 479, 639, 671
BseMI GCAATG 2 cut(s) 115, 907
BseNI ACTGG 2 cut(s) 582, 1168
BseRI GAGGAG 2 cut(s) 55, 622
BseSI GKGCMC 1 cut(s) 645
BseXI GCAGC 1 cut(s) 564
BseYI CCCAGC 1 cut(s) 644
Bsh1236I CGCG 3 cut(s) 177, 196, 225
BshFI GGCC 3 cut(s) 643, 760, 816
BshNI GGYRCC 1 cut(s) 468
BsiSI CCGG 2 cut(s) 105, 639
BslFI GGGAC 1 cut(s) 342
BslI CCNNNNNNNGG 6 cut(s) 197, 200, 419, 479, 639, 671
BsmAI GTCTC 3 cut(s) 527, 634, 988
BsmFI GGGAC 1 cut(s) 342
BsmI GAATGC 1 cut(s) 911
BsnI GGCC 3 cut(s) 643, 760, 816
Bso31I GGTCTC 1 cut(s) 527
Bsp120I GGGCCC 1 cut(s) 641
Bsp1286I GDGCHC 1 cut(s) 645
Bsp1407I TGTACA 1 cut(s) 731
Bsp143I GATC 5 cut(s) 178, 406, 601, 1084, 1201
BspANI GGCC 3 cut(s) 643, 760, 816
BspFNI CGCG 3 cut(s) 177, 196, 225
BspLI GGNNCC 5 cut(s) 330, 408, 470, 603, 643
BspMI ACCTGC 1 cut(s) 959
BspPI GGATC 7 cut(s) 173, 401, 414, 596, 609, 1092, 1209
BspQI GCTCTTC 2 cut(s) 120, 213
BspT107I GGYRCC 1 cut(s) 468
BspTNI GGTCTC 1 cut(s) 527
BsrDI GCAATG 2 cut(s) 115, 907
BsrFI RCCGGY 1 cut(s) 104
BsrGI TGTACA 1 cut(s) 731
BsrI ACTGG 2 cut(s) 582, 1168
BssAI RCCGGY 1 cut(s) 104
BssECI CCNNGG 1 cut(s) 478
BssMI GATC 5 cut(s) 178, 406, 601, 1084, 1201
BssNAI GTATAC 1 cut(s) 1143
BssNI GRCGYC 1 cut(s) 134
Bst1107I GTATAC 1 cut(s) 1143
Bst2UI CCWGG 3 cut(s) 480, 762, 1153
Bst4CI ACNGT 5 cut(s) 360, 368, 1106, 1229, 1253
Bst6I CTCTTC 3 cut(s) 120, 213, 390
BstACI GRCGYC 1 cut(s) 134
BstAPI GCANNNNNTGC 2 cut(s) 561, 906
BstAUI TGTACA 1 cut(s) 731
BstBAI YACGTR 1 cut(s) 684
BstC8I GCNNGC 3 cut(s) 173, 288, 1096
BstENI CCTNNNNNAGG 1 cut(s) 477
BstF5I GGATG 5 cut(s) 316, 635, 997, 1001, 1274
BstFNI CGCG 3 cut(s) 177, 196, 225
BstHHI GCGC 2 cut(s) 177, 225
BstKTI GATC 5 cut(s) 181, 409, 604, 1087, 1204
BstMAI GTCTC 3 cut(s) 527, 634, 988
BstMBI GATC 5 cut(s) 178, 406, 601, 1084, 1201
BstMWI GCNNNNNNNGC 4 cut(s) 127, 287, 561, 906
BstNI CCWGG 3 cut(s) 480, 762, 1153
BstNSI RCATGY 5 cut(s) 886, 936, 1065, 1098, 1141
BstPAI GACNNNNGTC 1 cut(s) 235
BstSCI CCNGG 4 cut(s) 478, 638, 760, 1151
BstSFI CTRYAG 1 cut(s) 910
BstSLI GKGCMC 1 cut(s) 645
BstUI CGCG 3 cut(s) 177, 196, 225
BstV1I GCAGC 1 cut(s) 564
BstV2I GAAGAC 3 cut(s) 684, 722, 1254
BstX2I RGATCY 3 cut(s) 406, 601, 1084
BstYI RGATCY 3 cut(s) 406, 601, 1084
BstZ17I GTATAC 1 cut(s) 1143
BsuRI GGCC 3 cut(s) 643, 760, 816
BtgZI GCGATG 2 cut(s) 240, 686
BtsCI GGATG 5 cut(s) 316, 635, 997, 1001, 1274
BtsIMutI CAGTG 3 cut(s) 373, 894, 1258
BveI ACCTGC 1 cut(s) 959
Cac8I GCNNGC 3 cut(s) 173, 288, 1096
CaiI CAGNNNCTG 1 cut(s) 1187
CfoI GCGC 2 cut(s) 177, 225
Cfr10I RCCGGY 1 cut(s) 104
Cfr13I GGNCC 7 cut(s) 52, 230, 329, 641, 642, 814, 838
CseI GACGC 1 cut(s) 142
Csp6I GTAC 4 cut(s) 356, 469, 732, 948
CviAII CATG 7 cut(s) 556, 883, 933, 980, 1062, 1095, 1138
CviQI GTAC 4 cut(s) 356, 469, 732, 948
DpnI GATC 5 cut(s) 180, 408, 603, 1086, 1203
DpnII GATC 5 cut(s) 178, 406, 601, 1084, 1201
DraI TTTAAA 1 cut(s) 348
DriI GACNNNNNGTC 1 cut(s) 626
Eam1104I CTCTTC 3 cut(s) 120, 213, 390
Eam1105I GACNNNNNGTC 1 cut(s) 626
EarI CTCTTC 3 cut(s) 120, 213, 390
EciI GGCGGA 1 cut(s) 105
Eco147I AGGCCT 1 cut(s) 760
Eco24I GRGCYC 1 cut(s) 645
Eco31I GGTCTC 1 cut(s) 527
Eco47I GGWCC 4 cut(s) 52, 230, 329, 838
Eco57I CTGAAG 2 cut(s) 59, 144
Eco72I CACGTG 1 cut(s) 684
EcoNI CCTNNNNNAGG 1 cut(s) 477
EcoO109I RGGNCCY 2 cut(s) 52, 329
EcoRII CCWGG 3 cut(s) 478, 760, 1151
EcoT38I GRGCYC 1 cut(s) 645
FaeI CATG 7 cut(s) 559, 886, 936, 983, 1065, 1098, 1141
FaqI GGGAC 1 cut(s) 342
FatI CATG 7 cut(s) 555, 882, 932, 979, 1061, 1094, 1137
FauI CCCGC 1 cut(s) 194
FauNDI CATATG 1 cut(s) 736
FblI GTMKAC 1 cut(s) 1142
Fnu4HI GCNGC 3 cut(s) 119, 194, 553
FokI GGATG 5 cut(s) 323, 622, 984, 988, 1261
FriOI GRGCYC 1 cut(s) 645
Fsp4HI GCNGC 3 cut(s) 119, 194, 553
GlaI GCGC 2 cut(s) 176, 224
GluI GCNGC 3 cut(s) 119, 194, 553
GsaI CCCAGC 1 cut(s) 648
HaeIII GGCC 3 cut(s) 643, 760, 816
HapII CCGG 2 cut(s) 105, 639
HgaI GACGC 1 cut(s) 142
HhaI GCGC 2 cut(s) 177, 225
Hin1I GRCGYC 1 cut(s) 134
Hin1II CATG 7 cut(s) 559, 886, 936, 983, 1065, 1098, 1141
Hin6I GCGC 2 cut(s) 175, 223
HinP1I GCGC 2 cut(s) 175, 223
HindIII AAGCTT 3 cut(s) 87, 273, 1116
HinfI GANTC 3 cut(s) 79, 434, 581
HpaII CCGG 2 cut(s) 105, 639
HphI GGTGA 4 cut(s) 467, 686, 799, 1081
Hpy166II GTNNAC 3 cut(s) 167, 461, 1143
Hpy188I TCNGA 6 cut(s) 78, 145, 388, 439, 1299, 1319
Hpy188III TCNNGA 2 cut(s) 245, 776
Hpy8I GTNNAC 3 cut(s) 167, 461, 1143
Hpy99I CGWCG 1 cut(s) 10
HpyAV CCTTC 3 cut(s) 42, 43, 342
HpyCH4III ACNGT 5 cut(s) 360, 368, 1106, 1229, 1253
HpyCH4IV ACGT 2 cut(s) 683, 697
HpyF10VI GCNNNNNNNGC 4 cut(s) 127, 287, 561, 906
HpySE526I ACGT 2 cut(s) 683, 697
Hsp92I GRCGYC 1 cut(s) 134
Hsp92II CATG 7 cut(s) 559, 886, 936, 983, 1065, 1098, 1141
HspAI GCGC 2 cut(s) 175, 223
KpnI GGTACC 1 cut(s) 472
Kzo9I GATC 5 cut(s) 178, 406, 601, 1084, 1201
LguI GCTCTTC 2 cut(s) 120, 213
LmnI GCTCC 1 cut(s) 118
Lsp1109I GCAGC 1 cut(s) 564
LweI GCATC 5 cut(s) 136, 250, 651, 851, 1225
MaeII ACGT 2 cut(s) 683, 697
MaeIII GTNAC 3 cut(s) 959, 1069, 1304
MalI GATC 5 cut(s) 180, 408, 603, 1086, 1203
MboI GATC 5 cut(s) 178, 406, 601, 1084, 1201
MflI RGATCY 3 cut(s) 406, 601, 1084
MhlI GDGCHC 1 cut(s) 645
MluCI AATT 8 cut(s) 16, 213, 299, 349, 415, 572, 823, 1173
MmeI TCCRAC 1 cut(s) 725
MroXI GAANNNNTTC 1 cut(s) 1229
MseI TTAA 5 cut(s) 347, 588, 608, 920, 1080
MslI CAYNNNNRTG 3 cut(s) 739, 887, 978
MspA1I CMGCKG 2 cut(s) 466, 836
MspI CCGG 2 cut(s) 105, 639
MspR9I CCNGG 4 cut(s) 480, 640, 762, 1153
Mva1269I GAATGC 1 cut(s) 911
MvaI CCWGG 3 cut(s) 480, 762, 1153
MvnI CGCG 3 cut(s) 177, 196, 225
MwoI GCNNNNNNNGC 4 cut(s) 127, 287, 561, 906
NciI CCSGG 1 cut(s) 640
NdeI CATATG 1 cut(s) 736
NdeII GATC 5 cut(s) 178, 406, 601, 1084, 1201
NlaIII CATG 7 cut(s) 559, 886, 936, 983, 1065, 1098, 1141
NlaIV GGNNCC 5 cut(s) 330, 408, 470, 603, 643
NmuCI GTSAC 3 cut(s) 959, 1069, 1304
NspI RCATGY 5 cut(s) 886, 936, 1065, 1098, 1141
PaeI GCATGC 1 cut(s) 1098
PceI AGGCCT 1 cut(s) 760
PciI ACATGT 2 cut(s) 882, 1137
PciSI GCTCTTC 2 cut(s) 120, 213
PcsI WCGNNNNNNNCGW 1 cut(s) 11
PctI GAATGC 1 cut(s) 911
PdmI GAANNNNTTC 1 cut(s) 1229
PfeI GAWTC 3 cut(s) 79, 434, 581
PflMI CCANNNNNTGG 1 cut(s) 419
PkrI GCNGC 3 cut(s) 120, 195, 554
PmaCI CACGTG 1 cut(s) 684
PmlI CACGTG 1 cut(s) 684
Ppu21I YACGTR 1 cut(s) 684
PpuMI RGGWCCY 2 cut(s) 52, 329
PscI ACATGT 2 cut(s) 882, 1137
PshAI GACNNNNGTC 1 cut(s) 235
Psp5II RGGWCCY 2 cut(s) 52, 329
Psp6I CCWGG 3 cut(s) 478, 760, 1151
PspCI CACGTG 1 cut(s) 684
PspFI CCCAGC 1 cut(s) 644
PspGI CCWGG 3 cut(s) 478, 760, 1151
PspN4I GGNNCC 5 cut(s) 330, 408, 470, 603, 643
PspOMI GGGCCC 1 cut(s) 641
PspPI GGNCC 7 cut(s) 52, 230, 329, 641, 642, 814, 838
PspPPI RGGWCCY 2 cut(s) 52, 329
PstNI CAGNNNCTG 1 cut(s) 1187
PsuI RGATCY 3 cut(s) 406, 601, 1084
RsaI GTAC 4 cut(s) 357, 470, 733, 949
RsaNI GTAC 4 cut(s) 356, 469, 732, 948
RseI CAYNNNNRTG 3 cut(s) 739, 887, 978
SapI GCTCTTC 2 cut(s) 120, 213
SaqAI TTAA 5 cut(s) 347, 588, 608, 920, 1080
SatI GCNGC 3 cut(s) 119, 194, 553
Sau3AI GATC 5 cut(s) 178, 406, 601, 1084, 1201
Sau96I GGNCC 7 cut(s) 52, 230, 329, 641, 642, 814, 838
ScaI AGTACT 1 cut(s) 949
ScrFI CCNGG 4 cut(s) 480, 640, 762, 1153
SduI GDGCHC 1 cut(s) 645
SfaNI GCATC 5 cut(s) 136, 250, 651, 851, 1225
SfcI CTRYAG 1 cut(s) 910
SinI GGWCC 4 cut(s) 52, 230, 329, 838
SmiMI CAYNNNNRTG 3 cut(s) 739, 887, 978
SmlI CTYRAG 1 cut(s) 774
SmoI CTYRAG 1 cut(s) 774
SphI GCATGC 1 cut(s) 1098
Sse9I AATT 8 cut(s) 16, 213, 299, 349, 415, 572, 823, 1173
SseBI AGGCCT 1 cut(s) 760
SspI AATATT 1 cut(s) 1016
StuI AGGCCT 1 cut(s) 760
StyD4I CCNGG 4 cut(s) 478, 638, 760, 1151
TaaI ACNGT 5 cut(s) 360, 368, 1106, 1229, 1253
TaiI ACGT 2 cut(s) 686, 700
TaqI TCGA 3 cut(s) 255, 710, 1009
TaqII GACCGA 1 cut(s) 551
TasI AATT 8 cut(s) 16, 213, 299, 349, 415, 572, 823, 1173
TatI WGTACW 2 cut(s) 731, 947
TauI GCSGC 2 cut(s) 121, 196
TfiI GAWTC 3 cut(s) 79, 434, 581
Tru1I TTAA 5 cut(s) 347, 588, 608, 920, 1080
Tru9I TTAA 5 cut(s) 347, 588, 608, 920, 1080
TscAI CASTG 3 cut(s) 373, 894, 1258
TseFI GTSAC 3 cut(s) 959, 1069, 1304
TseI GCWGC 1 cut(s) 552
Tsp45I GTSAC 3 cut(s) 959, 1069, 1304
TspDTI ATGAA 6 cut(s) 447, 630, 708, 753, 918, 1238
TspGWI ACGGA 1 cut(s) 58
TspRI CASTG 3 cut(s) 373, 894, 1258
Van91I CCANNNNNTGG 1 cut(s) 419
VpaK11BI GGWCC 4 cut(s) 52, 230, 329, 838
XagI CCTNNNNNAGG 1 cut(s) 477
XapI RAATTY 3 cut(s) 16, 349, 415
XceI RCATGY 5 cut(s) 886, 936, 1065, 1098, 1141
XmiI GTMKAC 1 cut(s) 1142
XmnI GAANNNNTTC 1 cut(s) 1229
ZrmI AGTACT 1 cut(s) 949
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.