RLG00000003559

HIT zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0010570
Physical Location & Seq
Reverse (-)
49989003 .. 49992371
3369 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000003559

Sequence Viewer

Length: 1092 bp
ATGACGACGAGAACGAACTTCTACAAGAACCCTTCCATTACTTACAAGAAGAACCTCAGTCTCTCCTCCGTTCTTCAAAACCTCCAAGCTTACAACATCGCCACCGGCAATGCTCCGCCGCCCGAAGAACAACATCCGCCAGCCGCCGATGTCAAAACGACAGGTCGTAAACGCCAGCGCGATCCAAAACCGCCGCCGCAGCATTGTGGCGGGAACCGTGAAATTGAAGAGCGCAATGGACCTATGTCGCACCAGGATTACATAGACAAGAGAAGAAAAGAAGCTTTTGCAACGCCAGCTTTTGAAGAATTAACTGCGGATGTTTTGGGAAAGCAGGGAACTTCCAATTCATGTTTAACTTTGGTACAGTATGACAGTGATGAGAGTAGTTCTTCAGAATGTGAAGAGAAGCAGCATGATCCTCCAAATTCTGTTCACAGGAATGAATCTGATGGAGTAAAAAGCAGAAGTGAACAACGTTTCCCTCACCCAGGCGAACCTGTTTGTGTAATGTGCGGTAAATACGGAGAATATATATGCAATGAGACAGATGACGATATCTGCAGCATGGAGTGCAAAGCTGAGTTACTGGAAGCTCTTAAAGTTGTCAAGGATCCATTAAGCAATGGAAGACCAGATGTGTCCTCATCCGGGCCCAACTATACCTTACCCATGCCTGATTTTGGTGAAGACACTTGGGATTATGAACGTCATCGCTGGTCGAAGAAGACTTCCAGTCTTTGTACTTATGAATGCTGGAAATGCAAGAGGCCTGGACACCTTGCTCAAGATTGTTTGGTGATGACAAATAACCAGATGACATTGGGCCAAAGCAATTCCAATTCAATACCAGCGGACCTTCTTGCGTTGTACAGAAGATGCCACCAGATAAGAAAAAACATGTCAGTGGCAAAGTGCAGCGAATGCTACAGCTCATTAAGTTTGGCTACATGCCTTGATTGTAGGATTGCCCTCTGTGACAATGCAGGTCATTTGCATGAGCATATACAGAGACATACATCCCATCAACGATATTATTCTCATAAACTCTCTCGTCTGGTGGGTACTTACTTTTCCTTGCTGCTTAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-CCHC zf-HIT

Protein Analysis

364

Amino Acids

41.0

Weight (kDa)

6.73

Isoelectric Point (pI)

59.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-HIT PF04438 166 - 193 1.9e-06 HIT zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 977
AccII CGCG 1 cut(s) 180
AclI AACGTT 1 cut(s) 478
AclWI GGATC 4 cut(s) 176, 413, 608, 621
AcsI RAATTY 1 cut(s) 427
AcuI CTGAAG 1 cut(s) 378
AfaI GTAC 4 cut(s) 366, 745, 872, 1066
AfiI CCNNNNNNNGG 3 cut(s) 491, 651, 683
AflIII ACRYGT 1 cut(s) 900
AgsI TTSAA 4 cut(s) 77, 227, 305, 846
AhdI GACNNNNNGTC 1 cut(s) 735
AjnI CCWGG 3 cut(s) 252, 490, 772
AloI GAACNNNNNNTCC 2 cut(s) 465, 497
AluBI AGCT 7 cut(s) 89, 284, 299, 581, 596, 933, 1089
AluI AGCT 7 cut(s) 89, 284, 299, 581, 596, 933, 1089
Alw26I GTCTC 3 cut(s) 65, 539, 1006
AlwI GGATC 4 cut(s) 176, 413, 608, 621
AoxI GGCC 3 cut(s) 653, 770, 826
ApaI GGGCCC 1 cut(s) 657
ApeKI GCWGC 5 cut(s) 199, 412, 564, 918, 1081
ApoI RAATTY 1 cut(s) 427
AspLEI GCGC 2 cut(s) 180, 234
AspS9I GGNCC 5 cut(s) 239, 653, 654, 826, 856
AsuC2I CCSGG 1 cut(s) 652
AsuHPI GGTGA 3 cut(s) 479, 698, 811
AvaII GGWCC 2 cut(s) 239, 856
BaeGI GKGCMC 1 cut(s) 657
BamHI GGATCC 1 cut(s) 613
BanII GRGCYC 1 cut(s) 657
BbsI GAAGAC 3 cut(s) 637, 696, 734
BbvI GCAGC 5 cut(s) 211, 424, 576, 930, 1068
BccI CCATC 2 cut(s) 446, 1032
BciT130I CCWGG 3 cut(s) 254, 492, 774
BcnI CCSGG 1 cut(s) 652
BcoDI GTCTC 3 cut(s) 65, 539, 1006
BfmI CTRYAG 2 cut(s) 562, 928
BfuAI ACCTGC 1 cut(s) 977
BisI GCNGC 9 cut(s) 119, 144, 194, 197, 200, 413, 565, 919, 1082
BlpI GCTNAGC 1 cut(s) 1085
BlsI GCNGC 9 cut(s) 120, 145, 195, 198, 201, 414, 566, 920, 1083
Bme1390I CCNGG 4 cut(s) 254, 492, 652, 774
Bme18I GGWCC 2 cut(s) 239, 856
BmeRI GACNNNNNGTC 1 cut(s) 735
BmgT120I GGNCC 5 cut(s) 239, 653, 654, 826, 856
BmiI GGNNCC 3 cut(s) 215, 615, 655
BmrFI CCNGG 4 cut(s) 254, 492, 652, 774
BmsI GCATC 1 cut(s) 869
BoxI GACNNNNGTC 1 cut(s) 244
BpiI GAAGAC 3 cut(s) 637, 696, 734
Bpu1102I GCTNAGC 1 cut(s) 1085
BpuEI CTTGAG 1 cut(s) 771
BpuMI CCSGG 1 cut(s) 652
BsaJI CCNNGG 1 cut(s) 490
Bsc4I CCNNNNNNNGG 3 cut(s) 491, 651, 683
Bse118I RCCGGY 1 cut(s) 104
Bse1I ACTGG 2 cut(s) 594, 735
Bse3DI GCAATG 4 cut(s) 115, 241, 547, 631
BseBI CCWGG 3 cut(s) 254, 492, 774
BseDI CCNNGG 1 cut(s) 490
BseGI GGATG 4 cut(s) 133, 325, 647, 1019
BseLI CCNNNNNNNGG 3 cut(s) 491, 651, 683
BseMI GCAATG 4 cut(s) 115, 241, 547, 631
BseMII CTCAG 2 cut(s) 70, 573
BseNI ACTGG 2 cut(s) 594, 735
BseRI GAGGAG 1 cut(s) 55
BseSI GKGCMC 1 cut(s) 657
BseXI GCAGC 5 cut(s) 211, 424, 576, 930, 1068
BsgI GTGCAG 1 cut(s) 937
Bsh1236I CGCG 1 cut(s) 180
BshFI GGCC 3 cut(s) 655, 772, 828
BsiSI CCGG 2 cut(s) 105, 651
BslI CCNNNNNNNGG 3 cut(s) 491, 651, 683
BsmAI GTCTC 3 cut(s) 65, 539, 1006
BsmI GAATGC 2 cut(s) 758, 929
BsnI GGCC 3 cut(s) 655, 772, 828
Bsp120I GGGCCC 1 cut(s) 653
Bsp1286I GDGCHC 1 cut(s) 657
Bsp1407I TGTACA 1 cut(s) 870
Bsp143I GATC 3 cut(s) 181, 418, 613
Bsp1720I GCTNAGC 1 cut(s) 1085
BspANI GGCC 3 cut(s) 655, 772, 828
BspCNI CTCAG 2 cut(s) 69, 574
BspFNI CGCG 1 cut(s) 180
BspLI GGNNCC 3 cut(s) 215, 615, 655
BspMAI CTGCAG 1 cut(s) 566
BspMI ACCTGC 1 cut(s) 977
BspPI GGATC 4 cut(s) 176, 413, 608, 621
BspQI GCTCTTC 1 cut(s) 222
BsrDI GCAATG 4 cut(s) 115, 241, 547, 631
BsrFI RCCGGY 1 cut(s) 104
BsrGI TGTACA 1 cut(s) 870
BsrI ACTGG 2 cut(s) 594, 735
BssAI RCCGGY 1 cut(s) 104
BssECI CCNNGG 1 cut(s) 490
BssMI GATC 3 cut(s) 181, 418, 613
Bst2UI CCWGG 3 cut(s) 254, 492, 774
Bst4CI ACNGT 3 cut(s) 218, 369, 377
Bst6I CTCTTC 2 cut(s) 222, 399
BstAPI GCANNNNNTGC 2 cut(s) 573, 924
BstAUI TGTACA 1 cut(s) 870
BstC8I GCNNGC 3 cut(s) 141, 176, 297
BstDEI CTNAG 3 cut(s) 56, 582, 1085
BstENI CCTNNNNNAGG 1 cut(s) 489
BstF5I GGATG 4 cut(s) 133, 325, 647, 1019
BstFNI CGCG 1 cut(s) 180
BstHHI GCGC 2 cut(s) 180, 234
BstKTI GATC 3 cut(s) 184, 421, 616
BstMAI GTCTC 3 cut(s) 65, 539, 1006
BstMBI GATC 3 cut(s) 181, 418, 613
BstMWI GCNNNNNNNGC 5 cut(s) 199, 296, 573, 762, 924
BstNI CCWGG 3 cut(s) 254, 492, 774
BstNSI RCATGY 2 cut(s) 904, 954
BstPAI GACNNNNGTC 1 cut(s) 244
BstSCI CCNGG 4 cut(s) 252, 490, 650, 772
BstSFI CTRYAG 2 cut(s) 562, 928
BstSLI GKGCMC 1 cut(s) 657
BstUI CGCG 1 cut(s) 180
BstV1I GCAGC 5 cut(s) 211, 424, 576, 930, 1068
BstV2I GAAGAC 3 cut(s) 637, 696, 734
BstX2I RGATCY 1 cut(s) 613
BstYI RGATCY 1 cut(s) 613
BsuRI GGCC 3 cut(s) 655, 772, 828
BtgZI GCGATG 2 cut(s) 82, 698
BtsCI GGATG 4 cut(s) 133, 325, 647, 1019
BtsIMutI CAGTG 2 cut(s) 382, 912
BveI ACCTGC 1 cut(s) 977
Cac8I GCNNGC 3 cut(s) 141, 176, 297
CfoI GCGC 2 cut(s) 180, 234
Cfr10I RCCGGY 1 cut(s) 104
Cfr13I GGNCC 5 cut(s) 239, 653, 654, 826, 856
Csp6I GTAC 4 cut(s) 365, 744, 871, 1065
CviAII CATG 7 cut(s) 351, 416, 568, 673, 901, 951, 998
CviQI GTAC 4 cut(s) 365, 744, 871, 1065
DdeI CTNAG 3 cut(s) 56, 582, 1085
DpnI GATC 3 cut(s) 183, 420, 615
DpnII GATC 3 cut(s) 181, 418, 613
DriI GACNNNNNGTC 1 cut(s) 735
Eam1104I CTCTTC 2 cut(s) 222, 399
Eam1105I GACNNNNNGTC 1 cut(s) 735
EarI CTCTTC 2 cut(s) 222, 399
EciI GGCGGA 2 cut(s) 105, 126
Eco147I AGGCCT 1 cut(s) 772
Eco24I GRGCYC 1 cut(s) 657
Eco32I GATATC 1 cut(s) 559
Eco47I GGWCC 2 cut(s) 239, 856
Eco57I CTGAAG 1 cut(s) 378
EcoNI CCTNNNNNAGG 1 cut(s) 489
EcoRII CCWGG 3 cut(s) 252, 490, 772
EcoRV GATATC 1 cut(s) 559
EcoT38I GRGCYC 1 cut(s) 657
FaeI CATG 7 cut(s) 354, 419, 571, 676, 904, 954, 1001
FatI CATG 7 cut(s) 350, 415, 567, 672, 900, 950, 997
FauI CCCGC 1 cut(s) 203
Fnu4HI GCNGC 9 cut(s) 119, 144, 194, 197, 200, 413, 565, 919, 1082
FokI GGATG 4 cut(s) 120, 332, 634, 1006
FriOI GRGCYC 1 cut(s) 657
Fsp4HI GCNGC 9 cut(s) 119, 144, 194, 197, 200, 413, 565, 919, 1082
GlaI GCGC 2 cut(s) 179, 233
GluI GCNGC 9 cut(s) 119, 144, 194, 197, 200, 413, 565, 919, 1082
HaeIII GGCC 3 cut(s) 655, 772, 828
HapII CCGG 2 cut(s) 105, 651
HhaI GCGC 2 cut(s) 180, 234
Hin1II CATG 7 cut(s) 354, 419, 571, 676, 904, 954, 1001
Hin6I GCGC 2 cut(s) 178, 232
HinP1I GCGC 2 cut(s) 178, 232
HindIII AAGCTT 2 cut(s) 87, 282
HinfI GANTC 1 cut(s) 446
HpaII CCGG 2 cut(s) 105, 651
HphI GGTGA 3 cut(s) 479, 698, 811
Hpy166II GTNNAC 3 cut(s) 170, 436, 473
Hpy188I TCNGA 2 cut(s) 397, 451
Hpy188III TCNNGA 1 cut(s) 788
Hpy8I GTNNAC 3 cut(s) 170, 436, 473
Hpy99I CGWCG 1 cut(s) 10
HpyAV CCTTC 2 cut(s) 42, 869
HpyCH4III ACNGT 3 cut(s) 218, 369, 377
HpyCH4IV ACGT 2 cut(s) 478, 709
HpyCH4V TGCA 8 cut(s) 290, 540, 564, 576, 765, 918, 986, 997
HpyF10VI GCNNNNNNNGC 5 cut(s) 199, 296, 573, 762, 924
HpyF3I CTNAG 3 cut(s) 56, 582, 1085
HpySE526I ACGT 2 cut(s) 478, 709
Hsp92II CATG 7 cut(s) 354, 419, 571, 676, 904, 954, 1001
HspAI GCGC 2 cut(s) 178, 232
Kzo9I GATC 3 cut(s) 181, 418, 613
LguI GCTCTTC 1 cut(s) 222
LmnI GCTCC 1 cut(s) 118
Lsp1109I GCAGC 5 cut(s) 211, 424, 576, 930, 1068
LweI GCATC 1 cut(s) 869
MaeII ACGT 2 cut(s) 478, 709
MaeIII GTNAC 2 cut(s) 585, 977
MalI GATC 3 cut(s) 183, 420, 615
MboI GATC 3 cut(s) 181, 418, 613
MflI RGATCY 1 cut(s) 613
MhlI GDGCHC 1 cut(s) 657
MluCI AATT 6 cut(s) 222, 308, 346, 427, 835, 841
MnlI CCTC 8 cut(s) 65, 76, 92, 432, 495, 655, 762, 983
MseI TTAA 5 cut(s) 311, 356, 600, 620, 938
MslI CAYNNNNRTG 3 cut(s) 441, 905, 996
MspA1I CMGCKG 1 cut(s) 854
MspI CCGG 2 cut(s) 105, 651
MspR9I CCNGG 4 cut(s) 254, 492, 652, 774
Mva1269I GAATGC 2 cut(s) 758, 929
MvaI CCWGG 3 cut(s) 254, 492, 774
MvnI CGCG 1 cut(s) 180
MwoI GCNNNNNNNGC 5 cut(s) 199, 296, 573, 762, 924
NciI CCSGG 1 cut(s) 652
NdeII GATC 3 cut(s) 181, 418, 613
NlaIII CATG 7 cut(s) 354, 419, 571, 676, 904, 954, 1001
NlaIV GGNNCC 3 cut(s) 215, 615, 655
NmuCI GTSAC 1 cut(s) 977
NspI RCATGY 2 cut(s) 904, 954
PceI AGGCCT 1 cut(s) 772
PciI ACATGT 1 cut(s) 900
PciSI GCTCTTC 1 cut(s) 222
PcsI WCGNNNNNNNCGW 1 cut(s) 11
PctI GAATGC 2 cut(s) 758, 929
PfeI GAWTC 1 cut(s) 446
PkrI GCNGC 9 cut(s) 120, 145, 195, 198, 201, 414, 566, 920, 1083
PscI ACATGT 1 cut(s) 900
PshAI GACNNNNGTC 1 cut(s) 244
Psp1406I AACGTT 1 cut(s) 478
Psp6I CCWGG 3 cut(s) 252, 490, 772
PspGI CCWGG 3 cut(s) 252, 490, 772
PspN4I GGNNCC 3 cut(s) 215, 615, 655
PspOMI GGGCCC 1 cut(s) 653
PspPI GGNCC 5 cut(s) 239, 653, 654, 826, 856
PstI CTGCAG 1 cut(s) 566
PsuI RGATCY 1 cut(s) 613
RsaI GTAC 4 cut(s) 366, 745, 872, 1066
RsaNI GTAC 4 cut(s) 365, 744, 871, 1065
RseI CAYNNNNRTG 3 cut(s) 441, 905, 996
SapI GCTCTTC 1 cut(s) 222
SaqAI TTAA 5 cut(s) 311, 356, 600, 620, 938
SatI GCNGC 9 cut(s) 119, 144, 194, 197, 200, 413, 565, 919, 1082
Sau3AI GATC 3 cut(s) 181, 418, 613
Sau96I GGNCC 5 cut(s) 239, 653, 654, 826, 856
ScrFI CCNGG 4 cut(s) 254, 492, 652, 774
SduI GDGCHC 1 cut(s) 657
SfaNI GCATC 1 cut(s) 869
SfcI CTRYAG 2 cut(s) 562, 928
SinI GGWCC 2 cut(s) 239, 856
SmiMI CAYNNNNRTG 3 cut(s) 441, 905, 996
SmlI CTYRAG 1 cut(s) 786
SmoI CTYRAG 1 cut(s) 786
Sse9I AATT 6 cut(s) 222, 308, 346, 427, 835, 841
SseBI AGGCCT 1 cut(s) 772
StuI AGGCCT 1 cut(s) 772
StyD4I CCNGG 4 cut(s) 252, 490, 650, 772
TaaI ACNGT 3 cut(s) 218, 369, 377
TaiI ACGT 2 cut(s) 481, 712
TaqI TCGA 1 cut(s) 722
TasI AATT 6 cut(s) 222, 308, 346, 427, 835, 841
TatI WGTACW 2 cut(s) 743, 870
TauI GCSGC 4 cut(s) 121, 146, 196, 199
TfiI GAWTC 1 cut(s) 446
Tru1I TTAA 5 cut(s) 311, 356, 600, 620, 938
Tru9I TTAA 5 cut(s) 311, 356, 600, 620, 938
TscAI CASTG 2 cut(s) 382, 912
TseFI GTSAC 1 cut(s) 977
TseI GCWGC 5 cut(s) 199, 412, 564, 918, 1081
Tsp45I GTSAC 1 cut(s) 977
TspDTI ATGAA 4 cut(s) 339, 459, 720, 765
TspGWI ACGGA 2 cut(s) 58, 540
TspRI CASTG 2 cut(s) 382, 912
VpaK11BI GGWCC 2 cut(s) 239, 856
XagI CCTNNNNNAGG 1 cut(s) 489
XapI RAATTY 1 cut(s) 427
XceI RCATGY 2 cut(s) 904, 954
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.