Rh7DG209400

HIT zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0010570
Physical Location & Seq
Forward (+)
19991138 .. 19996418
5281 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG209400.1

Sequence Viewer

Length: 1308 bp
ATGACGACGAGGACGAATTTGTACAAGAACCCTTCCATTACGTACAAGAAGGACCTCAGTCTCTCCTCCGTTCTTCAAAACCTCCAAGCTTACAACATCGCCACCGGCAATGCTCCGCCTCCTGAAGAACATCCTCCGCCAGCCGCCGATGCCAAAACGGCAGGTCGTAAACGCCAGCGCGATCCAAAACCGCCGCCGCAGCATTGTGGCGGGAAGCGTGAAATTGAAGAGGGCGACGGACCTATGTCGCACCAGGATTACATAGACAAGAGAAGAAAAGAAGCTTCTGCAACGCCAGCTTTCGAAGAATTAACTGCGGATGTTTTGGGAAAGCAAGGAACTTCCAATTCATGTTTAAATTTGGTACAGTATGACAGTGGTGAGACTAGTTCTTCAGAATGTGAAGAGAAGCAGCATGATCCTCCAAATTCTGTTCACAGGAATGAATCTGATGGAGTAAAGAGCAGAAGTGAACAGCGTTTGCCTCACCCAGGCGAACCTGTTTGTGTAATGTGTGGTAAATACGGAGAATATATATGCAACGAGACGGATGACGACATCTGCAGCATGGAGTGCAAAGCTGAGTTACTGGAAGCTCTTCAAGTTGTCAAGGATCCATTAAGCAATGAAAGACCAGATGTCTCCTCATCCGGGCCCAACTATACCTTACCCATGCCTGATTTTGGTGAAGACACTTGGGATTATGAACGTCATCGCTGGTTGAAGAAGACTTCCAGTCTTTGTAGTTATGAATGCTGGAAATGCAAGAGGCCTGGACACCTTGCTCAAGATTGTTTGGTGATGACAAATAACCAGATGACATTGGGCCAAAGCAATTCCAATTCAATATCAGCGGACCTTCTTGCGTTGTACAGAAGATGCCACCAGATAGGAAAAAACATGTCAGTGGCAAAGTGCAGCGAATGCTACAGCTCATTAAGTTTGGCTACATGCCTTGATTGTAGGACTGCCCTCTGTGACAATGCAGGTCATTTGCATGAGCATATACAGAGACATACATCCCATCAACGATATTATTCTCATAAACTCTCTCGTCTGGTGAAATGCTGTAAATCAACATGCAAGGTGACTGACATCAAGGATCTTTTGGCATGCCAGTACTGTTTTGATAAAGCTTTTGATAAGTTCTATGACATGTATACTGCATCTTGGTATGAAAATAGTTGGCTGAAATTTGTGTATCTTTTCCACTTATATTTTGGATTAATTATATTTTGCTACTCATGTTTGGGCTGTTCCATTTCTTTTACTTATTTGATTTGTTATTTCTTACTGGTGTGTCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-CCHC zf-HIT

Protein Analysis

435

Amino Acids

49.32

Weight (kDa)

6.35

Isoelectric Point (pI)

54.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-HIT PF04438 166 - 193 2.4e-06 HIT zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 152, 977
AccI GTMKAC 1 cut(s) 1160
AccII CGCG 1 cut(s) 180
AciI CCGC 9 cut(s) 116, 137, 144, 191, 194, 197, 210, 317, 854
AclWI GGATC 5 cut(s) 176, 413, 608, 621, 1110
AcsI RAATTY 4 cut(s) 16, 358, 427, 1193
AcuI CTGAAG 2 cut(s) 144, 378
AfaI GTAC 5 cut(s) 23, 44, 366, 872, 1121
AfiI CCNNNNNNNGG 3 cut(s) 491, 651, 683
AflIII ACRYGT 2 cut(s) 900, 1155
AgsI TTSAA 5 cut(s) 77, 227, 602, 724, 846
AhdI GACNNNNNGTC 2 cut(s) 638, 735
AhlI ACTAGT 1 cut(s) 386
AjnI CCWGG 3 cut(s) 252, 490, 772
AluBI AGCT 7 cut(s) 89, 284, 299, 581, 596, 933, 1136
AluI AGCT 7 cut(s) 89, 284, 299, 581, 596, 933, 1136
Alw26I GTCTC 5 cut(s) 65, 377, 539, 646, 1006
AlwI GGATC 5 cut(s) 176, 413, 608, 621, 1110
AoxI GGCC 3 cut(s) 653, 770, 826
ApaI GGGCCC 1 cut(s) 657
ApeKI GCWGC 4 cut(s) 199, 412, 564, 918
ApoI RAATTY 4 cut(s) 16, 358, 427, 1193
AseI ATTAAT 1 cut(s) 1226
Asp700I GAANNNNTTC 1 cut(s) 597
AspLEI GCGC 1 cut(s) 180
AspS9I GGNCC 6 cut(s) 52, 239, 653, 654, 826, 856
AsuC2I CCSGG 1 cut(s) 652
AsuHPI GGTGA 6 cut(s) 392, 479, 698, 811, 1072, 1099
AsuII TTCGAA 1 cut(s) 303
AvaII GGWCC 3 cut(s) 52, 239, 856
BaeGI GKGCMC 1 cut(s) 657
BamHI GGATCC 1 cut(s) 613
BanII GRGCYC 1 cut(s) 657
BbsI GAAGAC 2 cut(s) 696, 734
BbvI GCAGC 4 cut(s) 211, 424, 576, 930
BccI CCATC 2 cut(s) 446, 1032
BceAI ACGGC 1 cut(s) 174
BciT130I CCWGG 3 cut(s) 254, 492, 774
BcnI CCSGG 1 cut(s) 652
BcoDI GTCTC 5 cut(s) 65, 377, 539, 646, 1006
BcuI ACTAGT 1 cut(s) 386
BfaI CTAG 1 cut(s) 387
BfmI CTRYAG 2 cut(s) 562, 928
BfuAI ACCTGC 2 cut(s) 152, 977
BglI GCCNNNNNGGC 1 cut(s) 158
BisI GCNGC 7 cut(s) 144, 194, 197, 200, 413, 565, 919
BlsI GCNGC 7 cut(s) 145, 195, 198, 201, 414, 566, 920
BmcAI AGTACT 1 cut(s) 1121
Bme1390I CCNGG 4 cut(s) 254, 492, 652, 774
Bme18I GGWCC 3 cut(s) 52, 239, 856
BmeRI GACNNNNNGTC 2 cut(s) 638, 735
BmgT120I GGNCC 6 cut(s) 52, 239, 653, 654, 826, 856
BmiI GGNNCC 2 cut(s) 615, 655
BmrFI CCNGG 4 cut(s) 254, 492, 652, 774
BmsI GCATC 3 cut(s) 139, 869, 1175
BoxI GACNNNNGTC 2 cut(s) 57, 244
BpiI GAAGAC 2 cut(s) 696, 734
Bpu14I TTCGAA 1 cut(s) 303
BpuEI CTTGAG 1 cut(s) 771
BpuMI CCSGG 1 cut(s) 652
BsaAI YACGTR 1 cut(s) 42
BsaJI CCNNGG 1 cut(s) 490
Bsc4I CCNNNNNNNGG 3 cut(s) 491, 651, 683
Bse118I RCCGGY 1 cut(s) 104
Bse1I ACTGG 4 cut(s) 594, 735, 1117, 1299
Bse3DI GCAATG 2 cut(s) 115, 631
BseBI CCWGG 3 cut(s) 254, 492, 774
BseDI CCNNGG 1 cut(s) 490
BseGI GGATG 5 cut(s) 130, 325, 556, 647, 1019
BseLI CCNNNNNNNGG 3 cut(s) 491, 651, 683
BseMI GCAATG 2 cut(s) 115, 631
BseMII CTCAG 2 cut(s) 70, 573
BseNI ACTGG 4 cut(s) 594, 735, 1117, 1299
BseRI GAGGAG 2 cut(s) 55, 634
BseSI GKGCMC 1 cut(s) 657
BseXI GCAGC 4 cut(s) 211, 424, 576, 930
BsgI GTGCAG 1 cut(s) 937
Bsh1236I CGCG 1 cut(s) 180
BshFI GGCC 3 cut(s) 655, 772, 828
BsiSI CCGG 2 cut(s) 105, 651
BslI CCNNNNNNNGG 3 cut(s) 491, 651, 683
BsmAI GTCTC 5 cut(s) 65, 377, 539, 646, 1006
BsmBI CGTCTC 1 cut(s) 539
BsmI GAATGC 2 cut(s) 758, 929
BsnI GGCC 3 cut(s) 655, 772, 828
Bsp119I TTCGAA 1 cut(s) 303
Bsp120I GGGCCC 1 cut(s) 653
Bsp1286I GDGCHC 1 cut(s) 657
Bsp1407I TGTACA 2 cut(s) 21, 870
Bsp143I GATC 4 cut(s) 181, 418, 613, 1102
BspACI CCGC 9 cut(s) 116, 137, 144, 191, 194, 197, 210, 317, 854
BspANI GGCC 3 cut(s) 655, 772, 828
BspCNI CTCAG 2 cut(s) 69, 574
BspFNI CGCG 1 cut(s) 180
BspLI GGNNCC 2 cut(s) 615, 655
BspMAI CTGCAG 1 cut(s) 566
BspMI ACCTGC 2 cut(s) 152, 977
BspPI GGATC 5 cut(s) 176, 413, 608, 621, 1110
BspQI GCTCTTC 1 cut(s) 603
BspT104I TTCGAA 1 cut(s) 303
BsrDI GCAATG 2 cut(s) 115, 631
BsrFI RCCGGY 1 cut(s) 104
BsrGI TGTACA 2 cut(s) 21, 870
BsrI ACTGG 4 cut(s) 594, 735, 1117, 1299
BssAI RCCGGY 1 cut(s) 104
BssECI CCNNGG 1 cut(s) 490
BssMI GATC 4 cut(s) 181, 418, 613, 1102
BssNAI GTATAC 1 cut(s) 1161
Bst1107I GTATAC 1 cut(s) 1161
Bst2UI CCWGG 3 cut(s) 254, 492, 774
Bst4CI ACNGT 3 cut(s) 369, 377, 1124
Bst6I CTCTTC 3 cut(s) 222, 399, 603
BstAPI GCANNNNNTGC 2 cut(s) 573, 924
BstAUI TGTACA 2 cut(s) 21, 870
BstBAI YACGTR 1 cut(s) 42
BstBI TTCGAA 1 cut(s) 303
BstC8I GCNNGC 4 cut(s) 141, 176, 297, 1114
BstDEI CTNAG 2 cut(s) 56, 582
BstENI CCTNNNNNAGG 1 cut(s) 489
BstF5I GGATG 5 cut(s) 130, 325, 556, 647, 1019
BstFNI CGCG 1 cut(s) 180
BstHHI GCGC 1 cut(s) 180
BstKTI GATC 4 cut(s) 184, 421, 616, 1105
BstMAI GTCTC 5 cut(s) 65, 377, 539, 646, 1006
BstMBI GATC 4 cut(s) 181, 418, 613, 1102
BstMWI GCNNNNNNNGC 7 cut(s) 149, 158, 199, 296, 573, 762, 924
BstNI CCWGG 3 cut(s) 254, 492, 774
BstNSI RCATGY 5 cut(s) 904, 954, 1083, 1116, 1159
BstPAI GACNNNNGTC 2 cut(s) 57, 244
BstSCI CCNGG 4 cut(s) 252, 490, 650, 772
BstSFI CTRYAG 2 cut(s) 562, 928
BstSLI GKGCMC 1 cut(s) 657
BstSNI TACGTA 1 cut(s) 42
BstUI CGCG 1 cut(s) 180
BstV1I GCAGC 4 cut(s) 211, 424, 576, 930
BstV2I GAAGAC 2 cut(s) 696, 734
BstX2I RGATCY 2 cut(s) 613, 1102
BstYI RGATCY 2 cut(s) 613, 1102
BstZ17I GTATAC 1 cut(s) 1161
BsuRI GGCC 3 cut(s) 655, 772, 828
BtgZI GCGATG 2 cut(s) 82, 698
BtsCI GGATG 5 cut(s) 130, 325, 556, 647, 1019
BtsIMutI CAGTG 2 cut(s) 382, 912
BveI ACCTGC 2 cut(s) 152, 977
Cac8I GCNNGC 4 cut(s) 141, 176, 297, 1114
CfoI GCGC 1 cut(s) 180
Cfr10I RCCGGY 1 cut(s) 104
Cfr13I GGNCC 6 cut(s) 52, 239, 653, 654, 826, 856
Csp6I GTAC 5 cut(s) 22, 43, 365, 871, 1120
CviQI GTAC 5 cut(s) 22, 43, 365, 871, 1120
DdeI CTNAG 2 cut(s) 56, 582
DpnI GATC 4 cut(s) 183, 420, 615, 1104
DpnII GATC 4 cut(s) 181, 418, 613, 1102
DraI TTTAAA 1 cut(s) 357
DriI GACNNNNNGTC 2 cut(s) 638, 735
Eam1104I CTCTTC 3 cut(s) 222, 399, 603
Eam1105I GACNNNNNGTC 2 cut(s) 638, 735
EarI CTCTTC 3 cut(s) 222, 399, 603
EciI GGCGGA 2 cut(s) 105, 126
Eco105I TACGTA 1 cut(s) 42
Eco147I AGGCCT 1 cut(s) 772
Eco24I GRGCYC 1 cut(s) 657
Eco47I GGWCC 3 cut(s) 52, 239, 856
Eco57I CTGAAG 2 cut(s) 144, 378
EcoNI CCTNNNNNAGG 1 cut(s) 489
EcoO109I RGGNCCY 1 cut(s) 52
EcoRII CCWGG 3 cut(s) 252, 490, 772
EcoT38I GRGCYC 1 cut(s) 657
Esp3I CGTCTC 1 cut(s) 539
FauI CCCGC 1 cut(s) 203
FblI GTMKAC 1 cut(s) 1160
Fnu4HI GCNGC 7 cut(s) 144, 194, 197, 200, 413, 565, 919
FokI GGATG 5 cut(s) 117, 332, 563, 634, 1006
FriOI GRGCYC 1 cut(s) 657
Fsp4HI GCNGC 7 cut(s) 144, 194, 197, 200, 413, 565, 919
FspBI CTAG 1 cut(s) 387
GlaI GCGC 1 cut(s) 179
GluI GCNGC 7 cut(s) 144, 194, 197, 200, 413, 565, 919
HaeIII GGCC 3 cut(s) 655, 772, 828
HapII CCGG 2 cut(s) 105, 651
HhaI GCGC 1 cut(s) 180
Hin6I GCGC 1 cut(s) 178
HinP1I GCGC 1 cut(s) 178
HindIII AAGCTT 3 cut(s) 87, 282, 1134
HinfI GANTC 1 cut(s) 446
HpaII CCGG 2 cut(s) 105, 651
HphI GGTGA 6 cut(s) 392, 479, 698, 811, 1072, 1099
Hpy166II GTNNAC 4 cut(s) 170, 436, 473, 1161
Hpy188I TCNGA 2 cut(s) 397, 451
Hpy188III TCNNGA 2 cut(s) 122, 788
Hpy8I GTNNAC 4 cut(s) 170, 436, 473, 1161
Hpy99I CGWCG 2 cut(s) 10, 239
HpyAV CCTTC 3 cut(s) 42, 43, 869
HpyCH4III ACNGT 3 cut(s) 369, 377, 1124
HpyCH4IV ACGT 2 cut(s) 41, 709
HpyF10VI GCNNNNNNNGC 7 cut(s) 149, 158, 199, 296, 573, 762, 924
HpyF3I CTNAG 2 cut(s) 56, 582
HpySE526I ACGT 2 cut(s) 41, 709
HspAI GCGC 1 cut(s) 178
Kzo9I GATC 4 cut(s) 181, 418, 613, 1102
LguI GCTCTTC 1 cut(s) 603
LmnI GCTCC 1 cut(s) 118
Lsp1109I GCAGC 4 cut(s) 211, 424, 576, 930
LweI GCATC 3 cut(s) 139, 869, 1175
MaeI CTAG 1 cut(s) 387
MaeII ACGT 2 cut(s) 41, 709
MaeIII GTNAC 3 cut(s) 585, 977, 1087
MalI GATC 4 cut(s) 183, 420, 615, 1104
MboI GATC 4 cut(s) 181, 418, 613, 1102
MflI RGATCY 2 cut(s) 613, 1102
MhlI GDGCHC 1 cut(s) 657
MroXI GAANNNNTTC 1 cut(s) 597
MseI TTAA 5 cut(s) 311, 356, 620, 938, 1226
MslI CAYNNNNRTG 3 cut(s) 441, 905, 996
MspA1I CMGCKG 1 cut(s) 854
MspI CCGG 2 cut(s) 105, 651
MspR9I CCNGG 4 cut(s) 254, 492, 652, 774
Mva1269I GAATGC 2 cut(s) 758, 929
MvaI CCWGG 3 cut(s) 254, 492, 774
MvnI CGCG 1 cut(s) 180
MwoI GCNNNNNNNGC 7 cut(s) 149, 158, 199, 296, 573, 762, 924
NciI CCSGG 1 cut(s) 652
NdeII GATC 4 cut(s) 181, 418, 613, 1102
NlaIV GGNNCC 2 cut(s) 615, 655
NmuCI GTSAC 2 cut(s) 977, 1087
NspI RCATGY 5 cut(s) 904, 954, 1083, 1116, 1159
NspV TTCGAA 1 cut(s) 303
PaeI GCATGC 1 cut(s) 1116
PceI AGGCCT 1 cut(s) 772
PciI ACATGT 2 cut(s) 900, 1155
PciSI GCTCTTC 1 cut(s) 603
PcsI WCGNNNNNNNCGW 1 cut(s) 11
PctI GAATGC 2 cut(s) 758, 929
PdmI GAANNNNTTC 1 cut(s) 597
PfeI GAWTC 1 cut(s) 446
PkrI GCNGC 7 cut(s) 145, 195, 198, 201, 414, 566, 920
Ppu21I YACGTR 1 cut(s) 42
PpuMI RGGWCCY 1 cut(s) 52
PscI ACATGT 2 cut(s) 900, 1155
PshAI GACNNNNGTC 2 cut(s) 57, 244
PshBI ATTAAT 1 cut(s) 1226
Psp5II RGGWCCY 1 cut(s) 52
Psp6I CCWGG 3 cut(s) 252, 490, 772
PspGI CCWGG 3 cut(s) 252, 490, 772
PspN4I GGNNCC 2 cut(s) 615, 655
PspOMI GGGCCC 1 cut(s) 653
PspPI GGNCC 6 cut(s) 52, 239, 653, 654, 826, 856
PspPPI RGGWCCY 1 cut(s) 52
PstI CTGCAG 1 cut(s) 566
PsuI RGATCY 2 cut(s) 613, 1102
RsaI GTAC 5 cut(s) 23, 44, 366, 872, 1121
RsaNI GTAC 5 cut(s) 22, 43, 365, 871, 1120
RseI CAYNNNNRTG 3 cut(s) 441, 905, 996
SapI GCTCTTC 1 cut(s) 603
SaqAI TTAA 5 cut(s) 311, 356, 620, 938, 1226
SatI GCNGC 7 cut(s) 144, 194, 197, 200, 413, 565, 919
Sau3AI GATC 4 cut(s) 181, 418, 613, 1102
Sau96I GGNCC 6 cut(s) 52, 239, 653, 654, 826, 856
ScaI AGTACT 1 cut(s) 1121
ScrFI CCNGG 4 cut(s) 254, 492, 652, 774
SduI GDGCHC 1 cut(s) 657
SfaNI GCATC 3 cut(s) 139, 869, 1175
SfcI CTRYAG 2 cut(s) 562, 928
SfuI TTCGAA 1 cut(s) 303
SinI GGWCC 3 cut(s) 52, 239, 856
SmiMI CAYNNNNRTG 3 cut(s) 441, 905, 996
SmlI CTYRAG 1 cut(s) 786
SmoI CTYRAG 1 cut(s) 786
SnaBI TACGTA 1 cut(s) 42
SpeI ACTAGT 1 cut(s) 386
SphI GCATGC 1 cut(s) 1116
SseBI AGGCCT 1 cut(s) 772
SsiI CCGC 9 cut(s) 116, 137, 144, 191, 194, 197, 210, 317, 854
SspMI CTAG 1 cut(s) 387
StuI AGGCCT 1 cut(s) 772
StyD4I CCNGG 4 cut(s) 252, 490, 650, 772
TaaI ACNGT 3 cut(s) 369, 377, 1124
TaiI ACGT 2 cut(s) 44, 712
TaqI TCGA 1 cut(s) 303
TatI WGTACW 3 cut(s) 21, 870, 1119
TauI GCSGC 3 cut(s) 146, 196, 199
TfiI GAWTC 1 cut(s) 446
Tru1I TTAA 5 cut(s) 311, 356, 620, 938, 1226
Tru9I TTAA 5 cut(s) 311, 356, 620, 938, 1226
TscAI CASTG 2 cut(s) 382, 912
TseFI GTSAC 2 cut(s) 977, 1087
TseI GCWGC 4 cut(s) 199, 412, 564, 918
Tsp45I GTSAC 2 cut(s) 977, 1087
TspDTI ATGAA 6 cut(s) 339, 459, 642, 720, 765, 1191
TspGWI ACGGA 4 cut(s) 58, 252, 540, 563
TspRI CASTG 2 cut(s) 382, 912
VpaK11BI GGWCC 3 cut(s) 52, 239, 856
VspI ATTAAT 1 cut(s) 1226
XagI CCTNNNNNAGG 1 cut(s) 489
XapI RAATTY 4 cut(s) 16, 358, 427, 1193
XceI RCATGY 5 cut(s) 904, 954, 1083, 1116, 1159
XcmI CCANNNNNNNNNTGG 1 cut(s) 1217
XmiI GTMKAC 1 cut(s) 1160
XmnI GAANNNNTTC 1 cut(s) 597
XspI CTAG 1 cut(s) 387
ZrmI AGTACT 1 cut(s) 1121
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.