Rh7AG202300

HIT zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0010570
Physical Location & Seq
Forward (+)
18396381 .. 18401757
5377 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG202300.1

Sequence Viewer

Length: 987 bp
ATGACGACGAGGACGAATTTGTACAAGAACCCTTCCATTACGTACAAGAAGGACCTCAGTCTCTCCTCCGTTCTTCAAAACCTCCAAGCTTACAACATCGCCACCGGCAATGCCCCGCCTCCTGAAGAACATCCTCCGCCAGCCGCCGATGCCAAAACGGCAGGTCGTAAACGCCAGCGCGATCCAAAACCGCCGCCGCAGCATTGTGGCGGGAAGCGTGAAATTGAACAGCGCGACGGACCTATGTCGCACCAGGATTACATAGACAAGAGAAGAAAAGAAGCTTCTGCAACGCCAGCTTTCGAAGAATTAACTGCGGATGTTTTGGGAAAGCAAGGAACTTCCAATTCATGTTTAAATTTGGTACAGTATGACAGTGGTGAGACTAGTTCTTCAGAATGTGAAGAGAAGCAGCATGATCCTCCAAATTCTGTTCACAGGAATGAATCTGATGGAGTAAAGAGCAGAAGTGAACAGCGTTTGCCTCACCCAGGCGAACCTGTTTGTGTAATGTGTGGTAAATACGGAGAATATATATGCAACGAGACGGATGACGACATCTGCAGCATGGAGTGCAAAGCTGAGTTACTGGAAGCTCTTAAAGTTGTCAAGGATCCATTAAGCAATGAAAGACCAGATGTCTCCTCATCCGGGCCCAACTATACCTTACCCATGCCTGATTTTGGTGAAGACACTTGGGATGATGAACGTCATCGCTGGTTGAAGAAGACTTCCAGTCTTTGTAGTTATGAATGCTGGAAATGCAAGAGGCCTGGACACCTTGCTCAAGATTGTTTGGTGATGACAAATAACCAGATGACATTGGGCCAAAGCAATTCCAATTCAATATCAGCGGGCCTTCTTGCGTTGTACAGAAGATGCCACCAGATAGGAAAAAACATGTCAGTAGCAAAGTGCAGCGAATGCTACAGCTCATTAAGTTTGGCTACATGCCTTGATTGTAGGACTGCCCTCTGTGACAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-CCHC zf-HIT

Protein Analysis

328

Amino Acids

36.48

Weight (kDa)

6.03

Isoelectric Point (pI)

60.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-HIT PF04438 166 - 193 1.7e-06 HIT zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 152
AccII CGCG 2 cut(s) 180, 234
AciI CCGC 9 cut(s) 116, 137, 144, 191, 194, 197, 210, 317, 854
AclWI GGATC 4 cut(s) 176, 413, 608, 621
AcsI RAATTY 3 cut(s) 16, 358, 427
AcuI CTGAAG 2 cut(s) 144, 378
AfaI GTAC 4 cut(s) 23, 44, 366, 872
AfiI CCNNNNNNNGG 3 cut(s) 491, 651, 683
AflIII ACRYGT 1 cut(s) 900
AgsI TTSAA 4 cut(s) 77, 227, 724, 846
AhdI GACNNNNNGTC 2 cut(s) 638, 735
AhlI ACTAGT 1 cut(s) 386
AjnI CCWGG 3 cut(s) 252, 490, 772
AluBI AGCT 6 cut(s) 89, 284, 299, 581, 596, 933
AluI AGCT 6 cut(s) 89, 284, 299, 581, 596, 933
Alw26I GTCTC 4 cut(s) 65, 377, 539, 646
AlwI GGATC 4 cut(s) 176, 413, 608, 621
AoxI GGCC 4 cut(s) 653, 770, 826, 856
ApaI GGGCCC 1 cut(s) 657
ApeKI GCWGC 4 cut(s) 199, 412, 564, 918
ApoI RAATTY 3 cut(s) 16, 358, 427
AspLEI GCGC 2 cut(s) 180, 234
AspS9I GGNCC 6 cut(s) 52, 239, 653, 654, 826, 856
AsuC2I CCSGG 1 cut(s) 652
AsuHPI GGTGA 4 cut(s) 392, 479, 698, 811
AsuII TTCGAA 1 cut(s) 303
AvaII GGWCC 2 cut(s) 52, 239
BaeGI GKGCMC 1 cut(s) 657
BamHI GGATCC 1 cut(s) 613
BanII GRGCYC 1 cut(s) 657
BbsI GAAGAC 2 cut(s) 696, 734
BbvI GCAGC 4 cut(s) 211, 424, 576, 930
BccI CCATC 1 cut(s) 446
BceAI ACGGC 1 cut(s) 174
BciT130I CCWGG 3 cut(s) 254, 492, 774
BcnI CCSGG 1 cut(s) 652
BcoDI GTCTC 4 cut(s) 65, 377, 539, 646
BcuI ACTAGT 1 cut(s) 386
BfaI CTAG 1 cut(s) 387
BfmI CTRYAG 2 cut(s) 562, 928
BfuAI ACCTGC 1 cut(s) 152
BglI GCCNNNNNGGC 1 cut(s) 158
BisI GCNGC 7 cut(s) 144, 194, 197, 200, 413, 565, 919
BlsI GCNGC 7 cut(s) 145, 195, 198, 201, 414, 566, 920
Bme1390I CCNGG 4 cut(s) 254, 492, 652, 774
Bme18I GGWCC 2 cut(s) 52, 239
BmeRI GACNNNNNGTC 2 cut(s) 638, 735
BmgT120I GGNCC 6 cut(s) 52, 239, 653, 654, 826, 856
BmiI GGNNCC 2 cut(s) 615, 655
BmrFI CCNGG 4 cut(s) 254, 492, 652, 774
BmsI GCATC 2 cut(s) 139, 869
BoxI GACNNNNGTC 2 cut(s) 57, 244
BpiI GAAGAC 2 cut(s) 696, 734
Bpu14I TTCGAA 1 cut(s) 303
BpuEI CTTGAG 1 cut(s) 771
BpuMI CCSGG 1 cut(s) 652
BsaAI YACGTR 1 cut(s) 42
BsaJI CCNNGG 1 cut(s) 490
Bsc4I CCNNNNNNNGG 3 cut(s) 491, 651, 683
Bse118I RCCGGY 1 cut(s) 104
Bse1I ACTGG 2 cut(s) 594, 735
Bse3DI GCAATG 2 cut(s) 115, 631
BseBI CCWGG 3 cut(s) 254, 492, 774
BseDI CCNNGG 1 cut(s) 490
BseGI GGATG 5 cut(s) 130, 325, 556, 647, 706
BseLI CCNNNNNNNGG 3 cut(s) 491, 651, 683
BseMI GCAATG 2 cut(s) 115, 631
BseMII CTCAG 2 cut(s) 70, 573
BseNI ACTGG 2 cut(s) 594, 735
BseRI GAGGAG 2 cut(s) 55, 634
BseSI GKGCMC 1 cut(s) 657
BseXI GCAGC 4 cut(s) 211, 424, 576, 930
BsgI GTGCAG 1 cut(s) 937
Bsh1236I CGCG 2 cut(s) 180, 234
BshFI GGCC 4 cut(s) 655, 772, 828, 858
BsiSI CCGG 2 cut(s) 105, 651
BslI CCNNNNNNNGG 3 cut(s) 491, 651, 683
BsmAI GTCTC 4 cut(s) 65, 377, 539, 646
BsmBI CGTCTC 1 cut(s) 539
BsmI GAATGC 2 cut(s) 758, 929
BsnI GGCC 4 cut(s) 655, 772, 828, 858
Bsp119I TTCGAA 1 cut(s) 303
Bsp120I GGGCCC 1 cut(s) 653
Bsp1286I GDGCHC 1 cut(s) 657
Bsp1407I TGTACA 2 cut(s) 21, 870
Bsp143I GATC 3 cut(s) 181, 418, 613
BspACI CCGC 9 cut(s) 116, 137, 144, 191, 194, 197, 210, 317, 854
BspANI GGCC 4 cut(s) 655, 772, 828, 858
BspCNI CTCAG 2 cut(s) 69, 574
BspFNI CGCG 2 cut(s) 180, 234
BspLI GGNNCC 2 cut(s) 615, 655
BspMAI CTGCAG 1 cut(s) 566
BspMI ACCTGC 1 cut(s) 152
BspPI GGATC 4 cut(s) 176, 413, 608, 621
BspT104I TTCGAA 1 cut(s) 303
BsrDI GCAATG 2 cut(s) 115, 631
BsrFI RCCGGY 1 cut(s) 104
BsrGI TGTACA 2 cut(s) 21, 870
BsrI ACTGG 2 cut(s) 594, 735
BssAI RCCGGY 1 cut(s) 104
BssECI CCNNGG 1 cut(s) 490
BssMI GATC 3 cut(s) 181, 418, 613
Bst2UI CCWGG 3 cut(s) 254, 492, 774
Bst4CI ACNGT 2 cut(s) 369, 377
Bst6I CTCTTC 1 cut(s) 399
BstAPI GCANNNNNTGC 2 cut(s) 573, 924
BstAUI TGTACA 2 cut(s) 21, 870
BstBAI YACGTR 1 cut(s) 42
BstBI TTCGAA 1 cut(s) 303
BstC8I GCNNGC 4 cut(s) 141, 176, 297, 856
BstDEI CTNAG 2 cut(s) 56, 582
BstENI CCTNNNNNAGG 1 cut(s) 489
BstF5I GGATG 5 cut(s) 130, 325, 556, 647, 706
BstFNI CGCG 2 cut(s) 180, 234
BstHHI GCGC 2 cut(s) 180, 234
BstKTI GATC 3 cut(s) 184, 421, 616
BstMAI GTCTC 4 cut(s) 65, 377, 539, 646
BstMBI GATC 3 cut(s) 181, 418, 613
BstMWI GCNNNNNNNGC 7 cut(s) 149, 158, 199, 296, 573, 762, 924
BstNI CCWGG 3 cut(s) 254, 492, 774
BstNSI RCATGY 2 cut(s) 904, 954
BstPAI GACNNNNGTC 2 cut(s) 57, 244
BstSCI CCNGG 4 cut(s) 252, 490, 650, 772
BstSFI CTRYAG 2 cut(s) 562, 928
BstSLI GKGCMC 1 cut(s) 657
BstSNI TACGTA 1 cut(s) 42
BstUI CGCG 2 cut(s) 180, 234
BstV1I GCAGC 4 cut(s) 211, 424, 576, 930
BstV2I GAAGAC 2 cut(s) 696, 734
BstX2I RGATCY 1 cut(s) 613
BstYI RGATCY 1 cut(s) 613
BsuRI GGCC 4 cut(s) 655, 772, 828, 858
BtgZI GCGATG 2 cut(s) 82, 698
BtsCI GGATG 5 cut(s) 130, 325, 556, 647, 706
BtsIMutI CAGTG 1 cut(s) 382
BveI ACCTGC 1 cut(s) 152
Cac8I GCNNGC 4 cut(s) 141, 176, 297, 856
CfoI GCGC 2 cut(s) 180, 234
Cfr10I RCCGGY 1 cut(s) 104
Cfr13I GGNCC 6 cut(s) 52, 239, 653, 654, 826, 856
Csp6I GTAC 4 cut(s) 22, 43, 365, 871
CviAII CATG 6 cut(s) 351, 416, 568, 673, 901, 951
CviQI GTAC 4 cut(s) 22, 43, 365, 871
DdeI CTNAG 2 cut(s) 56, 582
DpnI GATC 3 cut(s) 183, 420, 615
DpnII GATC 3 cut(s) 181, 418, 613
DraI TTTAAA 1 cut(s) 357
DriI GACNNNNNGTC 2 cut(s) 638, 735
Eam1104I CTCTTC 1 cut(s) 399
Eam1105I GACNNNNNGTC 2 cut(s) 638, 735
EarI CTCTTC 1 cut(s) 399
EciI GGCGGA 1 cut(s) 126
Eco105I TACGTA 1 cut(s) 42
Eco147I AGGCCT 1 cut(s) 772
Eco24I GRGCYC 1 cut(s) 657
Eco47I GGWCC 2 cut(s) 52, 239
Eco57I CTGAAG 2 cut(s) 144, 378
EcoNI CCTNNNNNAGG 1 cut(s) 489
EcoO109I RGGNCCY 1 cut(s) 52
EcoRII CCWGG 3 cut(s) 252, 490, 772
EcoT38I GRGCYC 1 cut(s) 657
Esp3I CGTCTC 1 cut(s) 539
FaeI CATG 6 cut(s) 354, 419, 571, 676, 904, 954
FatI CATG 6 cut(s) 350, 415, 567, 672, 900, 950
FauI CCCGC 3 cut(s) 123, 203, 847
Fnu4HI GCNGC 7 cut(s) 144, 194, 197, 200, 413, 565, 919
FokI GGATG 5 cut(s) 117, 332, 563, 634, 713
FriOI GRGCYC 1 cut(s) 657
Fsp4HI GCNGC 7 cut(s) 144, 194, 197, 200, 413, 565, 919
FspBI CTAG 1 cut(s) 387
GlaI GCGC 2 cut(s) 179, 233
GluI GCNGC 7 cut(s) 144, 194, 197, 200, 413, 565, 919
HaeIII GGCC 4 cut(s) 655, 772, 828, 858
HapII CCGG 2 cut(s) 105, 651
HhaI GCGC 2 cut(s) 180, 234
Hin1II CATG 6 cut(s) 354, 419, 571, 676, 904, 954
Hin6I GCGC 2 cut(s) 178, 232
HinP1I GCGC 2 cut(s) 178, 232
HindIII AAGCTT 2 cut(s) 87, 282
HinfI GANTC 1 cut(s) 446
HpaII CCGG 2 cut(s) 105, 651
HphI GGTGA 4 cut(s) 392, 479, 698, 811
Hpy166II GTNNAC 3 cut(s) 170, 436, 473
Hpy188I TCNGA 2 cut(s) 397, 451
Hpy188III TCNNGA 2 cut(s) 122, 788
Hpy8I GTNNAC 3 cut(s) 170, 436, 473
Hpy99I CGWCG 2 cut(s) 10, 239
HpyAV CCTTC 3 cut(s) 42, 43, 869
HpyCH4III ACNGT 2 cut(s) 369, 377
HpyCH4IV ACGT 2 cut(s) 41, 709
HpyCH4V TGCA 6 cut(s) 290, 540, 564, 576, 765, 918
HpyF10VI GCNNNNNNNGC 7 cut(s) 149, 158, 199, 296, 573, 762, 924
HpyF3I CTNAG 2 cut(s) 56, 582
HpySE526I ACGT 2 cut(s) 41, 709
Hsp92II CATG 6 cut(s) 354, 419, 571, 676, 904, 954
HspAI GCGC 2 cut(s) 178, 232
Kzo9I GATC 3 cut(s) 181, 418, 613
Lsp1109I GCAGC 4 cut(s) 211, 424, 576, 930
LweI GCATC 2 cut(s) 139, 869
MaeI CTAG 1 cut(s) 387
MaeII ACGT 2 cut(s) 41, 709
MaeIII GTNAC 2 cut(s) 585, 977
MalI GATC 3 cut(s) 183, 420, 615
MboI GATC 3 cut(s) 181, 418, 613
MflI RGATCY 1 cut(s) 613
MhlI GDGCHC 1 cut(s) 657
MluCI AATT 8 cut(s) 16, 222, 308, 346, 358, 427, 835, 841
MseI TTAA 5 cut(s) 311, 356, 600, 620, 938
MslI CAYNNNNRTG 1 cut(s) 441
MspA1I CMGCKG 1 cut(s) 854
MspI CCGG 2 cut(s) 105, 651
MspR9I CCNGG 4 cut(s) 254, 492, 652, 774
Mva1269I GAATGC 2 cut(s) 758, 929
MvaI CCWGG 3 cut(s) 254, 492, 774
MvnI CGCG 2 cut(s) 180, 234
MwoI GCNNNNNNNGC 7 cut(s) 149, 158, 199, 296, 573, 762, 924
NciI CCSGG 1 cut(s) 652
NdeII GATC 3 cut(s) 181, 418, 613
NlaIII CATG 6 cut(s) 354, 419, 571, 676, 904, 954
NlaIV GGNNCC 2 cut(s) 615, 655
NmuCI GTSAC 1 cut(s) 977
NspI RCATGY 2 cut(s) 904, 954
NspV TTCGAA 1 cut(s) 303
PceI AGGCCT 1 cut(s) 772
PciI ACATGT 1 cut(s) 900
PcsI WCGNNNNNNNCGW 1 cut(s) 11
PctI GAATGC 2 cut(s) 758, 929
PfeI GAWTC 1 cut(s) 446
PkrI GCNGC 7 cut(s) 145, 195, 198, 201, 414, 566, 920
Ppu21I YACGTR 1 cut(s) 42
PpuMI RGGWCCY 1 cut(s) 52
PscI ACATGT 1 cut(s) 900
PshAI GACNNNNGTC 2 cut(s) 57, 244
Psp5II RGGWCCY 1 cut(s) 52
Psp6I CCWGG 3 cut(s) 252, 490, 772
PspGI CCWGG 3 cut(s) 252, 490, 772
PspN4I GGNNCC 2 cut(s) 615, 655
PspOMI GGGCCC 1 cut(s) 653
PspPI GGNCC 6 cut(s) 52, 239, 653, 654, 826, 856
PspPPI RGGWCCY 1 cut(s) 52
PstI CTGCAG 1 cut(s) 566
PsuI RGATCY 1 cut(s) 613
RsaI GTAC 4 cut(s) 23, 44, 366, 872
RsaNI GTAC 4 cut(s) 22, 43, 365, 871
RseI CAYNNNNRTG 1 cut(s) 441
SaqAI TTAA 5 cut(s) 311, 356, 600, 620, 938
SatI GCNGC 7 cut(s) 144, 194, 197, 200, 413, 565, 919
Sau3AI GATC 3 cut(s) 181, 418, 613
Sau96I GGNCC 6 cut(s) 52, 239, 653, 654, 826, 856
ScrFI CCNGG 4 cut(s) 254, 492, 652, 774
SduI GDGCHC 1 cut(s) 657
SfaNI GCATC 2 cut(s) 139, 869
SfcI CTRYAG 2 cut(s) 562, 928
SfuI TTCGAA 1 cut(s) 303
SinI GGWCC 2 cut(s) 52, 239
SmiMI CAYNNNNRTG 1 cut(s) 441
SmlI CTYRAG 1 cut(s) 786
SmoI CTYRAG 1 cut(s) 786
SnaBI TACGTA 1 cut(s) 42
SpeI ACTAGT 1 cut(s) 386
Sse9I AATT 8 cut(s) 16, 222, 308, 346, 358, 427, 835, 841
SseBI AGGCCT 1 cut(s) 772
SsiI CCGC 9 cut(s) 116, 137, 144, 191, 194, 197, 210, 317, 854
SspMI CTAG 1 cut(s) 387
StuI AGGCCT 1 cut(s) 772
StyD4I CCNGG 4 cut(s) 252, 490, 650, 772
TaaI ACNGT 2 cut(s) 369, 377
TaiI ACGT 2 cut(s) 44, 712
TaqI TCGA 1 cut(s) 303
TasI AATT 8 cut(s) 16, 222, 308, 346, 358, 427, 835, 841
TatI WGTACW 2 cut(s) 21, 870
TauI GCSGC 3 cut(s) 146, 196, 199
TfiI GAWTC 1 cut(s) 446
Tru1I TTAA 5 cut(s) 311, 356, 600, 620, 938
Tru9I TTAA 5 cut(s) 311, 356, 600, 620, 938
TscAI CASTG 1 cut(s) 382
TseFI GTSAC 1 cut(s) 977
TseI GCWGC 4 cut(s) 199, 412, 564, 918
Tsp45I GTSAC 1 cut(s) 977
TspDTI ATGAA 5 cut(s) 339, 459, 642, 720, 765
TspGWI ACGGA 4 cut(s) 58, 252, 540, 563
TspRI CASTG 1 cut(s) 382
VpaK11BI GGWCC 2 cut(s) 52, 239
XagI CCTNNNNNAGG 1 cut(s) 489
XapI RAATTY 3 cut(s) 16, 358, 427
XceI RCATGY 2 cut(s) 904, 954
XspI CTAG 1 cut(s) 387
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.