Rh7CG213400

HIT zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0010570
Physical Location & Seq
Forward (+)
19315366 .. 19320645
5280 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG213400.1

Sequence Viewer

Length: 996 bp
ATGACGACGAGGACGAATTTCTACAAGAACCCTTCCATTACCTACAAGAAGGACCTCAGTCTCTCCTCCGTTCTTCAAAACCTCCAAGCTTACAACATCGCCACCGGCAATGCTCCGCCGCCCGAAGAACAACATCCGCCAGCCGCCGATGTCAAAACGACAGGTCGTAAACGCCAGCGCGATCCAAAACCGCCGCCGCAGCATTGTGGCGGGAAGCGAGAAATTGAAGAGCGCGATGGACCTATGTCGCACCAGGATTACATAGACAAGAGAAGAAAAGAAGCTTTTGCAACGCCAGCATTTGAAGAATTAACTGCGGATGTTTTGGGAAAGCAGGGAACTTCCAATTCATGTTTAAATTTGGTACAGTATGACAGTGATGAGAGTAGTTCTTCAGAATGTGAAGAGAAGCCGCATGATCCTCCAAATTCTGTTCACAGGAATGAATCTGATGGAGTAAAGAGCAGAAGTGAACAGCGTTTGCCTCACCCAGGCGAACCGGTTTGTGTAATGTGTGGTAAATACGGAGAATATATATGCAACGAGACGGATGACGACATCTGCAGCATGGAGTGCAAAGCTGAGTTACTGGACGCTCTCAAAGTTGTCAAGGATCCATTAAGCAATGAAAGACCAGATGTCTCCTCATCCGGGCCCAACTATACCTTACCCATGCCTGATTTTGATGAACACACTTGGGATTATGAACGTCATCGCTGGTCAAAGAAGACTTCCAGTCTTTGTACTTATGAATGCTGGAAATGCAAGAGGCCTGGACACCTTGCTCAAGATTGTTTGGTGATGACAAACAACCAGATGACATTGGGCCAAAGCAATTCCAATTCAATATCAGCGGACCTTCTTGCGTTGTACAGAAGATGCCACCAGATAGGAAAAAACATGTCAGTGGCAAAGTGCAGCGAATGCTACAGCTCATTAAGTTTGGCTACATGCCTTGATTGTAGGACTGCCCTCTGTGACAAGTCATTTGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-CCHC zf-HIT

Protein Analysis

331

Amino Acids

37.14

Weight (kDa)

5.85

Isoelectric Point (pI)

61.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-HIT PF04438 166 - 193 1.7e-06 HIT zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 180, 234
AclWI GGATC 4 cut(s) 176, 413, 608, 621
AcsI RAATTY 3 cut(s) 16, 358, 427
AcuI CTGAAG 1 cut(s) 378
AfaI GTAC 3 cut(s) 366, 745, 872
AfiI CCNNNNNNNGG 2 cut(s) 491, 651
AflIII ACRYGT 1 cut(s) 900
AgeI ACCGGT 1 cut(s) 499
AgsI TTSAA 4 cut(s) 77, 227, 305, 846
AhdI GACNNNNNGTC 2 cut(s) 638, 735
AjnI CCWGG 3 cut(s) 252, 490, 772
AluBI AGCT 4 cut(s) 89, 284, 581, 933
AluI AGCT 4 cut(s) 89, 284, 581, 933
Alw26I GTCTC 3 cut(s) 65, 539, 646
AlwI GGATC 4 cut(s) 176, 413, 608, 621
AoxI GGCC 3 cut(s) 653, 770, 826
ApaI GGGCCC 1 cut(s) 657
ApeKI GCWGC 3 cut(s) 199, 564, 918
ApoI RAATTY 3 cut(s) 16, 358, 427
ArsI GACNNNNNNTTYG 1 cut(s) 971
AsiGI ACCGGT 1 cut(s) 499
AspLEI GCGC 2 cut(s) 180, 234
AspS9I GGNCC 6 cut(s) 52, 239, 653, 654, 826, 856
AsuC2I CCSGG 1 cut(s) 652
AsuHPI GGTGA 2 cut(s) 479, 811
AvaII GGWCC 3 cut(s) 52, 239, 856
BaeGI GKGCMC 1 cut(s) 657
BamHI GGATCC 1 cut(s) 613
BanII GRGCYC 1 cut(s) 657
BbsI GAAGAC 1 cut(s) 734
BbvI GCAGC 3 cut(s) 211, 576, 930
BccI CCATC 2 cut(s) 230, 446
BciT130I CCWGG 3 cut(s) 254, 492, 774
BcnI CCSGG 1 cut(s) 652
BcoDI GTCTC 3 cut(s) 65, 539, 646
BfmI CTRYAG 2 cut(s) 562, 928
BisI GCNGC 8 cut(s) 119, 144, 194, 197, 200, 413, 565, 919
BlsI GCNGC 8 cut(s) 120, 145, 195, 198, 201, 414, 566, 920
Bme1390I CCNGG 4 cut(s) 254, 492, 652, 774
Bme18I GGWCC 3 cut(s) 52, 239, 856
BmeRI GACNNNNNGTC 2 cut(s) 638, 735
BmgT120I GGNCC 6 cut(s) 52, 239, 653, 654, 826, 856
BmiI GGNNCC 2 cut(s) 615, 655
BmrFI CCNGG 4 cut(s) 254, 492, 652, 774
BmsI GCATC 1 cut(s) 869
BoxI GACNNNNGTC 2 cut(s) 57, 244
BpiI GAAGAC 1 cut(s) 734
BpuEI CTTGAG 1 cut(s) 771
BpuMI CCSGG 1 cut(s) 652
BsaJI CCNNGG 1 cut(s) 490
BsaWI WCCGGW 1 cut(s) 499
Bsc4I CCNNNNNNNGG 2 cut(s) 491, 651
Bse118I RCCGGY 2 cut(s) 104, 499
Bse1I ACTGG 2 cut(s) 594, 735
Bse3DI GCAATG 2 cut(s) 115, 631
BseBI CCWGG 3 cut(s) 254, 492, 774
BseDI CCNNGG 1 cut(s) 490
BseGI GGATG 4 cut(s) 133, 325, 556, 647
BseLI CCNNNNNNNGG 2 cut(s) 491, 651
BseMI GCAATG 2 cut(s) 115, 631
BseMII CTCAG 2 cut(s) 70, 573
BseNI ACTGG 2 cut(s) 594, 735
BseRI GAGGAG 2 cut(s) 55, 634
BseSI GKGCMC 1 cut(s) 657
BseXI GCAGC 3 cut(s) 211, 576, 930
BsgI GTGCAG 1 cut(s) 937
Bsh1236I CGCG 2 cut(s) 180, 234
BshFI GGCC 3 cut(s) 655, 772, 828
BshTI ACCGGT 1 cut(s) 499
BsiSI CCGG 3 cut(s) 105, 500, 651
BslI CCNNNNNNNGG 2 cut(s) 491, 651
BsmAI GTCTC 3 cut(s) 65, 539, 646
BsmBI CGTCTC 1 cut(s) 539
BsmI GAATGC 2 cut(s) 758, 929
BsnI GGCC 3 cut(s) 655, 772, 828
Bsp120I GGGCCC 1 cut(s) 653
Bsp1286I GDGCHC 1 cut(s) 657
Bsp1407I TGTACA 1 cut(s) 870
Bsp143I GATC 3 cut(s) 181, 418, 613
BspANI GGCC 3 cut(s) 655, 772, 828
BspCNI CTCAG 2 cut(s) 69, 574
BspFNI CGCG 2 cut(s) 180, 234
BspLI GGNNCC 2 cut(s) 615, 655
BspMAI CTGCAG 1 cut(s) 566
BspPI GGATC 4 cut(s) 176, 413, 608, 621
BspQI GCTCTTC 1 cut(s) 222
BsrDI GCAATG 2 cut(s) 115, 631
BsrFI RCCGGY 2 cut(s) 104, 499
BsrGI TGTACA 1 cut(s) 870
BsrI ACTGG 2 cut(s) 594, 735
BssAI RCCGGY 2 cut(s) 104, 499
BssECI CCNNGG 1 cut(s) 490
BssMI GATC 3 cut(s) 181, 418, 613
Bst2UI CCWGG 3 cut(s) 254, 492, 774
Bst4CI ACNGT 2 cut(s) 369, 377
Bst6I CTCTTC 2 cut(s) 222, 399
BstAPI GCANNNNNTGC 2 cut(s) 573, 924
BstAUI TGTACA 1 cut(s) 870
BstC8I GCNNGC 3 cut(s) 141, 176, 297
BstDEI CTNAG 2 cut(s) 56, 582
BstENI CCTNNNNNAGG 1 cut(s) 489
BstF5I GGATG 4 cut(s) 133, 325, 556, 647
BstFNI CGCG 2 cut(s) 180, 234
BstHHI GCGC 2 cut(s) 180, 234
BstKTI GATC 3 cut(s) 184, 421, 616
BstMAI GTCTC 3 cut(s) 65, 539, 646
BstMBI GATC 3 cut(s) 181, 418, 613
BstMWI GCNNNNNNNGC 5 cut(s) 199, 296, 573, 762, 924
BstNI CCWGG 3 cut(s) 254, 492, 774
BstNSI RCATGY 2 cut(s) 904, 954
BstPAI GACNNNNGTC 2 cut(s) 57, 244
BstSCI CCNGG 4 cut(s) 252, 490, 650, 772
BstSFI CTRYAG 2 cut(s) 562, 928
BstSLI GKGCMC 1 cut(s) 657
BstUI CGCG 2 cut(s) 180, 234
BstV1I GCAGC 3 cut(s) 211, 576, 930
BstV2I GAAGAC 1 cut(s) 734
BstX2I RGATCY 1 cut(s) 613
BstYI RGATCY 1 cut(s) 613
BsuRI GGCC 3 cut(s) 655, 772, 828
BtgZI GCGATG 3 cut(s) 82, 249, 698
BtsCI GGATG 4 cut(s) 133, 325, 556, 647
BtsIMutI CAGTG 2 cut(s) 382, 912
Cac8I GCNNGC 3 cut(s) 141, 176, 297
CfoI GCGC 2 cut(s) 180, 234
Cfr10I RCCGGY 2 cut(s) 104, 499
Cfr13I GGNCC 6 cut(s) 52, 239, 653, 654, 826, 856
CseI GACGC 1 cut(s) 602
Csp6I GTAC 3 cut(s) 365, 744, 871
CspAI ACCGGT 1 cut(s) 499
CviAII CATG 7 cut(s) 351, 416, 568, 673, 901, 951, 993
CviQI GTAC 3 cut(s) 365, 744, 871
DdeI CTNAG 2 cut(s) 56, 582
DpnI GATC 3 cut(s) 183, 420, 615
DpnII GATC 3 cut(s) 181, 418, 613
DraI TTTAAA 1 cut(s) 357
DriI GACNNNNNGTC 2 cut(s) 638, 735
Eam1104I CTCTTC 2 cut(s) 222, 399
Eam1105I GACNNNNNGTC 2 cut(s) 638, 735
EarI CTCTTC 2 cut(s) 222, 399
EciI GGCGGA 2 cut(s) 105, 126
Eco147I AGGCCT 1 cut(s) 772
Eco24I GRGCYC 1 cut(s) 657
Eco47I GGWCC 3 cut(s) 52, 239, 856
Eco57I CTGAAG 1 cut(s) 378
EcoNI CCTNNNNNAGG 1 cut(s) 489
EcoO109I RGGNCCY 1 cut(s) 52
EcoRII CCWGG 3 cut(s) 252, 490, 772
EcoT38I GRGCYC 1 cut(s) 657
Esp3I CGTCTC 1 cut(s) 539
FaeI CATG 7 cut(s) 354, 419, 571, 676, 904, 954, 996
FatI CATG 7 cut(s) 350, 415, 567, 672, 900, 950, 992
FauI CCCGC 1 cut(s) 203
Fnu4HI GCNGC 8 cut(s) 119, 144, 194, 197, 200, 413, 565, 919
FokI GGATG 4 cut(s) 120, 332, 563, 634
FriOI GRGCYC 1 cut(s) 657
Fsp4HI GCNGC 8 cut(s) 119, 144, 194, 197, 200, 413, 565, 919
GlaI GCGC 2 cut(s) 179, 233
GluI GCNGC 8 cut(s) 119, 144, 194, 197, 200, 413, 565, 919
HaeIII GGCC 3 cut(s) 655, 772, 828
HapII CCGG 3 cut(s) 105, 500, 651
HgaI GACGC 1 cut(s) 602
HhaI GCGC 2 cut(s) 180, 234
Hin1II CATG 7 cut(s) 354, 419, 571, 676, 904, 954, 996
Hin6I GCGC 2 cut(s) 178, 232
HinP1I GCGC 2 cut(s) 178, 232
HindIII AAGCTT 2 cut(s) 87, 282
HinfI GANTC 1 cut(s) 446
HpaII CCGG 3 cut(s) 105, 500, 651
HphI GGTGA 2 cut(s) 479, 811
Hpy166II GTNNAC 3 cut(s) 170, 436, 473
Hpy188I TCNGA 2 cut(s) 397, 451
Hpy188III TCNNGA 1 cut(s) 788
Hpy8I GTNNAC 3 cut(s) 170, 436, 473
Hpy99I CGWCG 1 cut(s) 10
HpyAV CCTTC 3 cut(s) 42, 43, 869
HpyCH4III ACNGT 2 cut(s) 369, 377
HpyCH4IV ACGT 1 cut(s) 709
HpyCH4V TGCA 7 cut(s) 290, 540, 564, 576, 765, 918, 992
HpyF10VI GCNNNNNNNGC 5 cut(s) 199, 296, 573, 762, 924
HpyF3I CTNAG 2 cut(s) 56, 582
HpySE526I ACGT 1 cut(s) 709
Hsp92II CATG 7 cut(s) 354, 419, 571, 676, 904, 954, 996
HspAI GCGC 2 cut(s) 178, 232
Kzo9I GATC 3 cut(s) 181, 418, 613
LguI GCTCTTC 1 cut(s) 222
LmnI GCTCC 1 cut(s) 118
Lsp1109I GCAGC 3 cut(s) 211, 576, 930
LweI GCATC 1 cut(s) 869
MaeII ACGT 1 cut(s) 709
MaeIII GTNAC 2 cut(s) 585, 977
MalI GATC 3 cut(s) 183, 420, 615
MboI GATC 3 cut(s) 181, 418, 613
MboII GAAGA 9 cut(s) 65, 137, 239, 285, 317, 384, 416, 739, 888
MflI RGATCY 1 cut(s) 613
MhlI GDGCHC 1 cut(s) 657
MluCI AATT 8 cut(s) 16, 222, 308, 346, 358, 427, 835, 841
MnlI CCTC 9 cut(s) 3, 65, 76, 92, 432, 495, 655, 762, 983
MseI TTAA 4 cut(s) 311, 356, 620, 938
MslI CAYNNNNRTG 3 cut(s) 441, 905, 991
MspA1I CMGCKG 1 cut(s) 854
MspI CCGG 3 cut(s) 105, 500, 651
MspR9I CCNGG 4 cut(s) 254, 492, 652, 774
Mva1269I GAATGC 2 cut(s) 758, 929
MvaI CCWGG 3 cut(s) 254, 492, 774
MvnI CGCG 2 cut(s) 180, 234
MwoI GCNNNNNNNGC 5 cut(s) 199, 296, 573, 762, 924
NciI CCSGG 1 cut(s) 652
NdeII GATC 3 cut(s) 181, 418, 613
NlaIII CATG 7 cut(s) 354, 419, 571, 676, 904, 954, 996
NlaIV GGNNCC 2 cut(s) 615, 655
NmuCI GTSAC 1 cut(s) 977
NspI RCATGY 2 cut(s) 904, 954
PceI AGGCCT 1 cut(s) 772
PciI ACATGT 1 cut(s) 900
PciSI GCTCTTC 1 cut(s) 222
PcsI WCGNNNNNNNCGW 1 cut(s) 11
PctI GAATGC 2 cut(s) 758, 929
PfeI GAWTC 1 cut(s) 446
PinAI ACCGGT 1 cut(s) 499
PkrI GCNGC 8 cut(s) 120, 145, 195, 198, 201, 414, 566, 920
PpuMI RGGWCCY 1 cut(s) 52
PscI ACATGT 1 cut(s) 900
PshAI GACNNNNGTC 2 cut(s) 57, 244
Psp5II RGGWCCY 1 cut(s) 52
Psp6I CCWGG 3 cut(s) 252, 490, 772
PspGI CCWGG 3 cut(s) 252, 490, 772
PspN4I GGNNCC 2 cut(s) 615, 655
PspOMI GGGCCC 1 cut(s) 653
PspPI GGNCC 6 cut(s) 52, 239, 653, 654, 826, 856
PspPPI RGGWCCY 1 cut(s) 52
PstI CTGCAG 1 cut(s) 566
PsuI RGATCY 1 cut(s) 613
RsaI GTAC 3 cut(s) 366, 745, 872
RsaNI GTAC 3 cut(s) 365, 744, 871
RseI CAYNNNNRTG 3 cut(s) 441, 905, 991
SapI GCTCTTC 1 cut(s) 222
SaqAI TTAA 4 cut(s) 311, 356, 620, 938
SatI GCNGC 8 cut(s) 119, 144, 194, 197, 200, 413, 565, 919
Sau3AI GATC 3 cut(s) 181, 418, 613
Sau96I GGNCC 6 cut(s) 52, 239, 653, 654, 826, 856
ScrFI CCNGG 4 cut(s) 254, 492, 652, 774
SduI GDGCHC 1 cut(s) 657
SfaNI GCATC 1 cut(s) 869
SfcI CTRYAG 2 cut(s) 562, 928
SinI GGWCC 3 cut(s) 52, 239, 856
SmiMI CAYNNNNRTG 3 cut(s) 441, 905, 991
SmlI CTYRAG 1 cut(s) 786
SmoI CTYRAG 1 cut(s) 786
Sse9I AATT 8 cut(s) 16, 222, 308, 346, 358, 427, 835, 841
SseBI AGGCCT 1 cut(s) 772
StuI AGGCCT 1 cut(s) 772
StyD4I CCNGG 4 cut(s) 252, 490, 650, 772
TaaI ACNGT 2 cut(s) 369, 377
TaiI ACGT 1 cut(s) 712
TasI AATT 8 cut(s) 16, 222, 308, 346, 358, 427, 835, 841
TatI WGTACW 2 cut(s) 743, 870
TauI GCSGC 5 cut(s) 121, 146, 196, 199, 415
TfiI GAWTC 1 cut(s) 446
Tru1I TTAA 4 cut(s) 311, 356, 620, 938
Tru9I TTAA 4 cut(s) 311, 356, 620, 938
TscAI CASTG 2 cut(s) 382, 912
TseFI GTSAC 1 cut(s) 977
TseI GCWGC 3 cut(s) 199, 564, 918
Tsp45I GTSAC 1 cut(s) 977
TspDTI ATGAA 6 cut(s) 339, 459, 642, 702, 720, 765
TspGWI ACGGA 3 cut(s) 58, 540, 563
TspRI CASTG 2 cut(s) 382, 912
VpaK11BI GGWCC 3 cut(s) 52, 239, 856
XagI CCTNNNNNAGG 1 cut(s) 489
XapI RAATTY 3 cut(s) 16, 358, 427
XceI RCATGY 2 cut(s) 904, 954
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.