Rh7BG202400

HIT zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0010570
Physical Location & Seq
Forward (+)
16854393 .. 16859865
5473 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG202400.1

Sequence Viewer

Length: 1095 bp
ATGACGACGAGGACGAATTTCTACAAGAACCCTTCCATTACTTACAAGAAGGACCTCAGTCTCTCCTCCGTTCTTCAAAACCTCCAAGCTTACAACATCGCCACCGGCAATGCTCCGCCTCCCGAAGAACAACATCCTCCGCCAGCCGCCGATGCCAAAACGGCAGGTCGTAAACGCCAGCGCGATCCAAAACCGCCGCCGCAGCATTGTGGCGGGAAGCGTGAAATTGAAGAGCGCGACGGACCTATGTCGCACCAGGATTACGTAGACAAGAGAAGAAAAGAAGCTTCTGCAACGCCAGCTTTTGAAGAATTAACTGCGGATGTTTTGGGAAAGCAGGGAACTTCGAATTCATGTTTAAATTTGGTACAGTATGACAGTGATGAGAGTAGTTCTTCAGAATGTGAAGAGAAGCAGCATGATCCTTCTAATTCTGTTCACAGGAATGAATCTGATGGAGTAAAGAGCAGAAGTGAACAGCGTTTCCCTCACCCAGGCGAACCTGTTTGTGTAATGTGCGGTAAATACGGAGAATATATATGCAACGAGACGGATGACGACATCTGCAGCATGGAGTGCAAAGCTGAGTTACTGGACGCTCTCAAAGTTGTCAAGGATCCATTAAGCAATGAAAGACCAGATGTCTCCTCATCCGGTCCCAACTATACCTTACCCATGCCTGATTTTGGTGAAGACACTTGGGATTATGAACGTCATCGCTGGTCGAAGAAGACTTCCAGTCTTTGTACTTATGAATGCTGGAAATGCAAGAGGCCTGGACACCTTGCTCAAGATTGTTTGGTGATGACAAATAACCAGATGACATTGGGCCAAAGCAATTCCAATTCAATACCAGCGGACCTTCTTGCGTTGTACAGAAGATGCCACCAGATAGGAAAAAACATGTCAGTGGCAAAGTGCAGCGAATGCTACAGCTCATTAAGTTTGGCTACATGCCTTGATTGTAGGACTGCCCTCTGTGACAATGCAGGTCATTTGCATGAGCATATACAGAGACATACATCCCATCAACGATATTATTCTCATAAACTCTCTCGTCTGGTGGGTACTTACTTTGCCTTGCTGCTTAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-CCHC zf-HIT

Protein Analysis

364

Amino Acids

40.91

Weight (kDa)

6.31

Isoelectric Point (pI)

60.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-HIT PF04438 167 - 194 1.9e-06 HIT zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 155, 980
AccI GTMKAC 1 cut(s) 267
AccII CGCG 2 cut(s) 183, 237
AclWI GGATC 4 cut(s) 179, 416, 611, 624
AcsI RAATTY 3 cut(s) 16, 349, 361
AcuI CTGAAG 1 cut(s) 381
AfaI GTAC 4 cut(s) 369, 748, 875, 1069
AfiI CCNNNNNNNGG 2 cut(s) 494, 686
AflIII ACRYGT 1 cut(s) 903
AgsI TTSAA 4 cut(s) 77, 230, 308, 849
AhdI GACNNNNNGTC 2 cut(s) 641, 738
AjnI CCWGG 3 cut(s) 255, 493, 775
AloI GAACNNNNNNTCC 2 cut(s) 468, 500
AluBI AGCT 6 cut(s) 89, 287, 302, 584, 936, 1092
AluI AGCT 6 cut(s) 89, 287, 302, 584, 936, 1092
Alw26I GTCTC 4 cut(s) 65, 542, 649, 1009
AlwI GGATC 4 cut(s) 179, 416, 611, 624
AoxI GGCC 2 cut(s) 773, 829
ApeKI GCWGC 5 cut(s) 202, 415, 567, 921, 1084
ApoI RAATTY 3 cut(s) 16, 349, 361
AspLEI GCGC 2 cut(s) 183, 237
AspS9I GGNCC 5 cut(s) 52, 242, 656, 829, 859
AsuHPI GGTGA 3 cut(s) 482, 701, 814
AsuII TTCGAA 1 cut(s) 347
AvaII GGWCC 4 cut(s) 52, 242, 656, 859
BamHI GGATCC 1 cut(s) 616
BbsI GAAGAC 2 cut(s) 699, 737
BbvI GCAGC 5 cut(s) 214, 427, 579, 933, 1071
BccI CCATC 2 cut(s) 449, 1035
BceAI ACGGC 1 cut(s) 177
BciT130I CCWGG 3 cut(s) 257, 495, 777
BcoDI GTCTC 4 cut(s) 65, 542, 649, 1009
BfmI CTRYAG 2 cut(s) 565, 931
BfuAI ACCTGC 2 cut(s) 155, 980
BglI GCCNNNNNGGC 1 cut(s) 161
BisI GCNGC 8 cut(s) 147, 197, 200, 203, 416, 568, 922, 1085
BlpI GCTNAGC 1 cut(s) 1088
BlsI GCNGC 8 cut(s) 148, 198, 201, 204, 417, 569, 923, 1086
Bme1390I CCNGG 3 cut(s) 257, 495, 777
Bme18I GGWCC 4 cut(s) 52, 242, 656, 859
BmeRI GACNNNNNGTC 2 cut(s) 641, 738
BmgT120I GGNCC 5 cut(s) 52, 242, 656, 829, 859
BmiI GGNNCC 2 cut(s) 618, 658
BmrFI CCNGG 3 cut(s) 257, 495, 777
BmsI GCATC 2 cut(s) 142, 872
BoxI GACNNNNGTC 2 cut(s) 57, 247
BpiI GAAGAC 2 cut(s) 699, 737
Bpu1102I GCTNAGC 1 cut(s) 1088
Bpu14I TTCGAA 1 cut(s) 347
BpuEI CTTGAG 1 cut(s) 774
BsaAI YACGTR 1 cut(s) 265
BsaJI CCNNGG 1 cut(s) 493
BsaWI WCCGGW 1 cut(s) 653
Bsc4I CCNNNNNNNGG 2 cut(s) 494, 686
Bse118I RCCGGY 1 cut(s) 104
Bse1I ACTGG 2 cut(s) 597, 738
Bse3DI GCAATG 2 cut(s) 115, 634
BseBI CCWGG 3 cut(s) 257, 495, 777
BseDI CCNNGG 1 cut(s) 493
BseGI GGATG 5 cut(s) 133, 328, 559, 650, 1022
BseLI CCNNNNNNNGG 2 cut(s) 494, 686
BseMI GCAATG 2 cut(s) 115, 634
BseMII CTCAG 2 cut(s) 70, 576
BseNI ACTGG 2 cut(s) 597, 738
BseRI GAGGAG 2 cut(s) 55, 637
BseXI GCAGC 5 cut(s) 214, 427, 579, 933, 1071
BsgI GTGCAG 1 cut(s) 940
Bsh1236I CGCG 2 cut(s) 183, 237
BshFI GGCC 2 cut(s) 775, 831
BsiSI CCGG 2 cut(s) 105, 654
BslFI GGGAC 1 cut(s) 642
BslI CCNNNNNNNGG 2 cut(s) 494, 686
BsmAI GTCTC 4 cut(s) 65, 542, 649, 1009
BsmBI CGTCTC 1 cut(s) 542
BsmFI GGGAC 1 cut(s) 642
BsmI GAATGC 2 cut(s) 761, 932
BsnI GGCC 2 cut(s) 775, 831
Bsp119I TTCGAA 1 cut(s) 347
Bsp1407I TGTACA 1 cut(s) 873
Bsp143I GATC 3 cut(s) 184, 421, 616
Bsp1720I GCTNAGC 1 cut(s) 1088
BspANI GGCC 2 cut(s) 775, 831
BspCNI CTCAG 2 cut(s) 69, 577
BspFNI CGCG 2 cut(s) 183, 237
BspLI GGNNCC 2 cut(s) 618, 658
BspMAI CTGCAG 1 cut(s) 569
BspMI ACCTGC 2 cut(s) 155, 980
BspPI GGATC 4 cut(s) 179, 416, 611, 624
BspQI GCTCTTC 1 cut(s) 225
BspT104I TTCGAA 1 cut(s) 347
BsrDI GCAATG 2 cut(s) 115, 634
BsrFI RCCGGY 1 cut(s) 104
BsrGI TGTACA 1 cut(s) 873
BsrI ACTGG 2 cut(s) 597, 738
BssAI RCCGGY 1 cut(s) 104
BssECI CCNNGG 1 cut(s) 493
BssMI GATC 3 cut(s) 184, 421, 616
Bst2UI CCWGG 3 cut(s) 257, 495, 777
Bst4CI ACNGT 2 cut(s) 372, 380
Bst6I CTCTTC 2 cut(s) 225, 402
BstAPI GCANNNNNTGC 2 cut(s) 576, 927
BstAUI TGTACA 1 cut(s) 873
BstBAI YACGTR 1 cut(s) 265
BstBI TTCGAA 1 cut(s) 347
BstC8I GCNNGC 3 cut(s) 144, 179, 300
BstDEI CTNAG 3 cut(s) 56, 585, 1088
BstENI CCTNNNNNAGG 1 cut(s) 492
BstF5I GGATG 5 cut(s) 133, 328, 559, 650, 1022
BstFNI CGCG 2 cut(s) 183, 237
BstHHI GCGC 2 cut(s) 183, 237
BstKTI GATC 3 cut(s) 187, 424, 619
BstMAI GTCTC 4 cut(s) 65, 542, 649, 1009
BstMBI GATC 3 cut(s) 184, 421, 616
BstMWI GCNNNNNNNGC 7 cut(s) 152, 161, 202, 299, 576, 765, 927
BstNI CCWGG 3 cut(s) 257, 495, 777
BstNSI RCATGY 2 cut(s) 907, 957
BstPAI GACNNNNGTC 2 cut(s) 57, 247
BstSCI CCNGG 3 cut(s) 255, 493, 775
BstSFI CTRYAG 2 cut(s) 565, 931
BstSNI TACGTA 1 cut(s) 265
BstUI CGCG 2 cut(s) 183, 237
BstV1I GCAGC 5 cut(s) 214, 427, 579, 933, 1071
BstV2I GAAGAC 2 cut(s) 699, 737
BstX2I RGATCY 1 cut(s) 616
BstYI RGATCY 1 cut(s) 616
BsuRI GGCC 2 cut(s) 775, 831
BtgZI GCGATG 2 cut(s) 82, 701
BtsCI GGATG 5 cut(s) 133, 328, 559, 650, 1022
BtsIMutI CAGTG 2 cut(s) 385, 915
BveI ACCTGC 2 cut(s) 155, 980
Cac8I GCNNGC 3 cut(s) 144, 179, 300
CfoI GCGC 2 cut(s) 183, 237
Cfr10I RCCGGY 1 cut(s) 104
Cfr13I GGNCC 5 cut(s) 52, 242, 656, 829, 859
CseI GACGC 1 cut(s) 605
Csp6I GTAC 4 cut(s) 368, 747, 874, 1068
CviAII CATG 7 cut(s) 354, 419, 571, 676, 904, 954, 1001
CviQI GTAC 4 cut(s) 368, 747, 874, 1068
DdeI CTNAG 3 cut(s) 56, 585, 1088
DpnI GATC 3 cut(s) 186, 423, 618
DpnII GATC 3 cut(s) 184, 421, 616
DraI TTTAAA 1 cut(s) 360
DriI GACNNNNNGTC 2 cut(s) 641, 738
Eam1104I CTCTTC 2 cut(s) 225, 402
Eam1105I GACNNNNNGTC 2 cut(s) 641, 738
EarI CTCTTC 2 cut(s) 225, 402
EciI GGCGGA 2 cut(s) 105, 129
Eco105I TACGTA 1 cut(s) 265
Eco147I AGGCCT 1 cut(s) 775
Eco47I GGWCC 4 cut(s) 52, 242, 656, 859
Eco57I CTGAAG 1 cut(s) 381
EcoNI CCTNNNNNAGG 1 cut(s) 492
EcoO109I RGGNCCY 1 cut(s) 52
EcoRI GAATTC 1 cut(s) 349
EcoRII CCWGG 3 cut(s) 255, 493, 775
Esp3I CGTCTC 1 cut(s) 542
FaeI CATG 7 cut(s) 357, 422, 574, 679, 907, 957, 1004
FaqI GGGAC 1 cut(s) 642
FatI CATG 7 cut(s) 353, 418, 570, 675, 903, 953, 1000
FauI CCCGC 1 cut(s) 206
FblI GTMKAC 1 cut(s) 267
Fnu4HI GCNGC 8 cut(s) 147, 197, 200, 203, 416, 568, 922, 1085
FokI GGATG 5 cut(s) 120, 335, 566, 637, 1009
Fsp4HI GCNGC 8 cut(s) 147, 197, 200, 203, 416, 568, 922, 1085
GlaI GCGC 2 cut(s) 182, 236
GluI GCNGC 8 cut(s) 147, 197, 200, 203, 416, 568, 922, 1085
HaeIII GGCC 2 cut(s) 775, 831
HapII CCGG 2 cut(s) 105, 654
HgaI GACGC 1 cut(s) 605
HhaI GCGC 2 cut(s) 183, 237
Hin1II CATG 7 cut(s) 357, 422, 574, 679, 907, 957, 1004
Hin6I GCGC 2 cut(s) 181, 235
HinP1I GCGC 2 cut(s) 181, 235
HindIII AAGCTT 2 cut(s) 87, 285
HinfI GANTC 1 cut(s) 449
HpaII CCGG 2 cut(s) 105, 654
HphI GGTGA 3 cut(s) 482, 701, 814
Hpy166II GTNNAC 4 cut(s) 173, 268, 439, 476
Hpy188I TCNGA 2 cut(s) 400, 454
Hpy188III TCNNGA 2 cut(s) 122, 791
Hpy8I GTNNAC 4 cut(s) 173, 268, 439, 476
Hpy99I CGWCG 2 cut(s) 10, 242
HpyAV CCTTC 4 cut(s) 42, 43, 435, 872
HpyCH4III ACNGT 2 cut(s) 372, 380
HpyCH4IV ACGT 2 cut(s) 264, 712
HpyCH4V TGCA 8 cut(s) 293, 543, 567, 579, 768, 921, 989, 1000
HpyF10VI GCNNNNNNNGC 7 cut(s) 152, 161, 202, 299, 576, 765, 927
HpyF3I CTNAG 3 cut(s) 56, 585, 1088
HpySE526I ACGT 2 cut(s) 264, 712
Hsp92II CATG 7 cut(s) 357, 422, 574, 679, 907, 957, 1004
HspAI GCGC 2 cut(s) 181, 235
Kzo9I GATC 3 cut(s) 184, 421, 616
LguI GCTCTTC 1 cut(s) 225
LmnI GCTCC 1 cut(s) 118
Lsp1109I GCAGC 5 cut(s) 214, 427, 579, 933, 1071
LweI GCATC 2 cut(s) 142, 872
MaeII ACGT 2 cut(s) 264, 712
MaeIII GTNAC 2 cut(s) 588, 980
MalI GATC 3 cut(s) 186, 423, 618
MboI GATC 3 cut(s) 184, 421, 616
MflI RGATCY 1 cut(s) 616
MluCI AATT 8 cut(s) 16, 225, 311, 349, 361, 430, 838, 844
MseI TTAA 4 cut(s) 314, 359, 623, 941
MslI CAYNNNNRTG 3 cut(s) 444, 908, 999
MspA1I CMGCKG 1 cut(s) 857
MspI CCGG 2 cut(s) 105, 654
MspR9I CCNGG 3 cut(s) 257, 495, 777
Mva1269I GAATGC 2 cut(s) 761, 932
MvaI CCWGG 3 cut(s) 257, 495, 777
MvnI CGCG 2 cut(s) 183, 237
MwoI GCNNNNNNNGC 7 cut(s) 152, 161, 202, 299, 576, 765, 927
NdeII GATC 3 cut(s) 184, 421, 616
NlaIII CATG 7 cut(s) 357, 422, 574, 679, 907, 957, 1004
NlaIV GGNNCC 2 cut(s) 618, 658
NmuCI GTSAC 1 cut(s) 980
NspI RCATGY 2 cut(s) 907, 957
NspV TTCGAA 1 cut(s) 347
PceI AGGCCT 1 cut(s) 775
PciI ACATGT 1 cut(s) 903
PciSI GCTCTTC 1 cut(s) 225
PcsI WCGNNNNNNNCGW 1 cut(s) 11
PctI GAATGC 2 cut(s) 761, 932
PfeI GAWTC 1 cut(s) 449
PkrI GCNGC 8 cut(s) 148, 198, 201, 204, 417, 569, 923, 1086
Ppu21I YACGTR 1 cut(s) 265
PpuMI RGGWCCY 1 cut(s) 52
PscI ACATGT 1 cut(s) 903
PshAI GACNNNNGTC 2 cut(s) 57, 247
Psp5II RGGWCCY 1 cut(s) 52
Psp6I CCWGG 3 cut(s) 255, 493, 775
PspGI CCWGG 3 cut(s) 255, 493, 775
PspN4I GGNNCC 2 cut(s) 618, 658
PspPI GGNCC 5 cut(s) 52, 242, 656, 829, 859
PspPPI RGGWCCY 1 cut(s) 52
PstI CTGCAG 1 cut(s) 569
PsuI RGATCY 1 cut(s) 616
RsaI GTAC 4 cut(s) 369, 748, 875, 1069
RsaNI GTAC 4 cut(s) 368, 747, 874, 1068
RseI CAYNNNNRTG 3 cut(s) 444, 908, 999
SapI GCTCTTC 1 cut(s) 225
SaqAI TTAA 4 cut(s) 314, 359, 623, 941
SatI GCNGC 8 cut(s) 147, 197, 200, 203, 416, 568, 922, 1085
Sau3AI GATC 3 cut(s) 184, 421, 616
Sau96I GGNCC 5 cut(s) 52, 242, 656, 829, 859
ScrFI CCNGG 3 cut(s) 257, 495, 777
SfaNI GCATC 2 cut(s) 142, 872
SfcI CTRYAG 2 cut(s) 565, 931
SfuI TTCGAA 1 cut(s) 347
SinI GGWCC 4 cut(s) 52, 242, 656, 859
SmiMI CAYNNNNRTG 3 cut(s) 444, 908, 999
SmlI CTYRAG 1 cut(s) 789
SmoI CTYRAG 1 cut(s) 789
SnaBI TACGTA 1 cut(s) 265
Sse9I AATT 8 cut(s) 16, 225, 311, 349, 361, 430, 838, 844
SseBI AGGCCT 1 cut(s) 775
StuI AGGCCT 1 cut(s) 775
StyD4I CCNGG 3 cut(s) 255, 493, 775
TaaI ACNGT 2 cut(s) 372, 380
TaiI ACGT 2 cut(s) 267, 715
TaqI TCGA 2 cut(s) 347, 725
TasI AATT 8 cut(s) 16, 225, 311, 349, 361, 430, 838, 844
TatI WGTACW 2 cut(s) 746, 873
TauI GCSGC 3 cut(s) 149, 199, 202
TfiI GAWTC 1 cut(s) 449
Tru1I TTAA 4 cut(s) 314, 359, 623, 941
Tru9I TTAA 4 cut(s) 314, 359, 623, 941
TscAI CASTG 2 cut(s) 385, 915
TseFI GTSAC 1 cut(s) 980
TseI GCWGC 5 cut(s) 202, 415, 567, 921, 1084
Tsp45I GTSAC 1 cut(s) 980
TspDTI ATGAA 5 cut(s) 342, 462, 645, 723, 768
TspGWI ACGGA 4 cut(s) 58, 255, 543, 566
TspRI CASTG 2 cut(s) 385, 915
VpaK11BI GGWCC 4 cut(s) 52, 242, 656, 859
XagI CCTNNNNNAGG 1 cut(s) 492
XapI RAATTY 3 cut(s) 16, 349, 361
XceI RCATGY 2 cut(s) 907, 957
XmiI GTMKAC 1 cut(s) 267
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.