Rorug07G0074000

Scarecrow-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0002481
Physical Location & Seq
Reverse (-)
5702511 .. 5707464
4954 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0074000.1

Sequence Viewer

Length: 2241 bp
ATGGGGAGTTACAGTGAAGAGTGTGAGGATCAATTTTATGATACCAGGGAGGACCTATCTTCTGTGTCAGATTGGGGTGCAGATTGCTGTGAGGACTGCAGTCCTATTGCTTCGGTTTTTTCGAGGTATGAGTGTTGGGGTAAGAACCCGGAAAGTGTTCATGAGCGCCGCCATAGGTTCCTCAAATGGATGGGTTTGGATTTAGATCGGAATTTGAGTATGACAGAAGAATCAGATGATGGCTTCTTTATTGACAGAAGTGAAATGGGTATTGATAGAATGAAAGAAGACAGTGGGGTGGTGTTGAGAACTTCAGGATTTGAAGAGGGGTGTTGCTCAAATCAGTGTACAGTGTCTTCTTCATCAAGTGAAGTTCGAATATCACTAGGAAATGCTTTGGATGAGAATTTTGAATTTCCAACGAGGAAATTGAATGATCGAAGGGACTTTGTTGTGGATGAATTGGGTGAGAATTGCATACTCAGCAGATTCCGCGAGTTGAGACTAAATCAGTTGGTCAGTTCTGGGGAGATTCATTCCAACCGTGCACCAGCACCATCTCCTTCAATTCAGCAATTATTGCAGAGAGAAGTTGAAAACGCTAGGTACTTGCTTGATGCAAAAAAGAAGACTAAAAGAGGATGGTTAAAGAAGTTACGCAGTGGGATGTGTGTTGCTGATAAACCTGAGGCTGCACTGAGCCCCATCAGTCTTCAATCTGCAACACGGACAGGGATGCAAAGAGTCTGGGTTCAATCAAGTAAAAAGCGGAGCAAGGAACTATCTTCTCTTTATGGTGGGCAAAATTTTTCGGCTCATAAGGGGTCAATTTTGTCGATGAAGTTCAGTCTTGATGGGCAACACCTGGCAAGTGCTGGTCAAGATGGCATCGTCCGTGTATGGAAGGTGATTGAGGATGAGAGAATAGACAAATTTGATATTGCGAATGATCCTTCTTCTCTATACTTCAAAATGAATCCATTTTCTAAGTTAGGCTCTCAGGTTGTGGACAAAGAGAAACAAAGTAATGCGAAGAAATTGGGTGGATCATCAGACTCAGCTTGTGTGATTTTCCCTCCAAAGATTTTCCGTTTAATGGAGAAGCCTCTGCACGAATTCCAGGGACACAGTGGTGATGTCTTGGATCTCTCATGGTCAAAGAATGGGTTTCTGCTCTCATCCTCTGTTGATAAGACAGTTCGACTATGGCAAGTGGGATGTGACAGATGCCTGAGAGTTTTCTCTCATAATAATTATGTGACATGTGTTGATTTCAACCCTATGGATGATAATTATTTCATCAGTGGTTCAATTGACGGAAAAGTTCGCATCTGGGAAATTATTCGTTGTCTGGTTGTGGATTATATTGATGTTCGCGAGATAGTCACTGCTGTATCTTATCATCCTGATGGAGAGGGAGGAATTGTGGGCTTTGTCACCGGAAATTGCTGCTTTTATAATATCATAGATAATCACCTGAAATTGGATGCTGAGATATGCATACAGGGCAAAAAGAAGTCAGCGGGAAAGAGGATTACTGGCTTGCAGTTTTATCCAAGCAACCCAAGCAAGGTTATGGTCACCTCTGCCGATTCAGTAGTACGTGTCATTTCTGGGTCTAAGGTCATCTGCAAATTTAAGGGTCTGCGAAGTGGAGGAAGCCATGTTTCTGCATCTTTTACCTCAGATGGAAACCACATTGTTTCGGCGAGTGAGGATTCAAGTGTTCACATCTGGAACTACAATAGCCAGGACACGTCCTCTTCACGATCAAAGAACATAAGGTCTTGTGAGAGTTTCGTGTCCCACAACTCGATGATCGCGATACCTTGGAATGGTGTGAACGATCTCCCAGGAACACTTCCATCCCCTACATTCATTGGGGATGCTCAGGGAAGAAGTAGCCTTGATAGTCCGCTGAAGCTTCACGATTTCGATGAGAGAGCACAACAGAAAATGCATCTCTCTTCACCTGATTGCTTCTCTATGGGTCGTGGGTTTTTGTTGGAGTCATTGCCAAAGGGAACTCCAACTTGGCCTGAAGAGAAACTTGTAAACTCAAGTCCCGTCCCCCCGGTGCCTGTTTCCCCAACAATGTCTAGATCAGAGTACAGGTTTTTGAAGAATGCTTGTCAGAGCATGTCTGCTTCTTCTCATATGTGGGGCCTCGTGATTGTCACTGCCGGCTGGGACGGGCATATCAGAACCTACCACAATTATGGGTTACCGACTCGTTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

746

Amino Acids

83.24

Weight (kDa)

6.69

Isoelectric Point (pI)

53.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR3_1st PF25173 245 - 309 3.1e-06 WDR3 first beta-propeller domain
Beta-prop_WDR3_2nd PF25172 268 - 446 7e-12 WDR3 second beta-propeller domain
WD40 PF00400 269 - 301 3.1e-07 WD domain, G-beta repeat
Beta-prop_WDR36-Utp21_2nd PF25168 269 - 323 4.4e-07 WDR36/Utp21 second beta-propeller domain
WD40_WDHD1_1st PF24817 269 - 319 2.1e-06 WDHD1 first WD40 domain
Beta-prop_THOC3 PF25174 269 - 313 5.6e-06 THOC3 beta-propeller domain
WD40_Prp19 PF24814 332 - 486 6.4e-15 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 349 - 587 1.7e-22 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 361 - 589 4.4e-31 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 365 - 447 1.5e-12 WDR3 first beta-propeller domain
WD40 PF00400 368 - 403 2e-08 WD domain, G-beta repeat
Beta-prop_WDR36-Utp21_2nd PF25168 369 - 430 2.2e-07 WDR36/Utp21 second beta-propeller domain
WD40_CDC20-Fz PF24807 369 - 479 1.3e-10 CDC20/Fizzy WD40 domain
Beta-prop_CAF1B_HIR1 PF24105 373 - 452 7.1e-11 CAF1B/HIR1 beta-propeller domain
EIF3I PF24805 373 - 583 1.5e-06 EIF3I
Beta-prop_WDR90_POC16_2nd PF23393 393 - 493 4.3e-06 WDR90/POC16, second beta-propeller
WD40 PF00400 409 - 445 4.3e-07 WD domain, G-beta repeat
Beta-prop_EML_2 PF23414 423 - 614 1.7e-13 Echinoderm microtubule-associated protein second beta-propeller
WD40_WDHD1_1st PF24817 522 - 612 6.9e-07 WDHD1 first WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1458
AccB1I GGYRCC 1 cut(s) 2077
AccII CGCG 3 cut(s) 495, 1377, 1823
AciI CCGC 5 cut(s) 169, 493, 769, 1523, 1916
AclWI GGATC 4 cut(s) 36, 944, 1054, 1152
AcsI RAATTY 7 cut(s) 211, 406, 413, 805, 932, 1115, 1634
AcuI CTGAAG 3 cut(s) 297, 1940, 2061
AfaI GTAC 4 cut(s) 349, 608, 1602, 2111
AfiI CCNNNNNNNGG 4 cut(s) 1096, 1483, 1570, 1835
AflIII ACRYGT 3 cut(s) 1262, 1603, 1755
AjiI CACGTC 1 cut(s) 1758
AjnI CCWGG 5 cut(s) 44, 864, 1119, 1749, 1852
AjuI GAANNNNNNNTTGG 2 cut(s) 2017, 2049
AleI CACNNNNGTG 1 cut(s) 1131
AluBI AGCT 2 cut(s) 1061, 1924
AluI AGCT 2 cut(s) 1061, 1924
Alw21I GWGCWC 2 cut(s) 550, 1948
Alw26I GTCTC 1 cut(s) 496
Alw44I GTGCAC 1 cut(s) 546
AlwI GGATC 4 cut(s) 36, 944, 1054, 1152
AoxI GGCC 2 cut(s) 2036, 2164
ApaLI GTGCAC 1 cut(s) 546
ApeKI GCWGC 2 cut(s) 692, 1449
ApoI RAATTY 7 cut(s) 211, 406, 413, 805, 932, 1115, 1634
Asp700I GAANNNNTTC 2 cut(s) 156, 1341
AspLEI GCGC 1 cut(s) 168
AspS9I GGNCC 2 cut(s) 52, 2164
AsuC2I CCSGG 2 cut(s) 149, 2075
AsuHPI GGTGA 7 cut(s) 479, 919, 1145, 1429, 1466, 1573, 1962
AsuII TTCGAA 1 cut(s) 376
AvaII GGWCC 1 cut(s) 52
AxyI CCTNAGG 1 cut(s) 687
BaeGI GKGCMC 1 cut(s) 550
BaeI ACNNNNGTAYC 2 cut(s) 1377, 1410
BanI GGYRCC 1 cut(s) 2077
BanII GRGCYC 1 cut(s) 704
BarI GAAGNNNNNNTAC 2 cut(s) 340, 372
BauI CACGAG 1 cut(s) 2168
BbsI GAAGAC 4 cut(s) 294, 348, 635, 704
Bbv12I GWGCWC 2 cut(s) 550, 1948
BbvI GCAGC 2 cut(s) 679, 1436
BciT130I CCWGG 5 cut(s) 46, 866, 1121, 1751, 1854
BcnI CCSGG 2 cut(s) 149, 2075
BcoDI GTCTC 1 cut(s) 496
BfaI CTAG 3 cut(s) 386, 603, 2100
BfmI CTRYAG 1 cut(s) 97
BfoI RGCGCY 1 cut(s) 169
BisI GCNGC 3 cut(s) 169, 693, 1450
BlsI GCNGC 3 cut(s) 170, 694, 1451
Bme1390I CCNGG 7 cut(s) 46, 149, 866, 1121, 1751, 1854, 2075
Bme18I GGWCC 1 cut(s) 52
BmgBI CACGTC 1 cut(s) 1758
BmgT120I GGNCC 2 cut(s) 52, 2164
BmiI GGNNCC 3 cut(s) 179, 2079, 2165
BmrFI CCNGG 7 cut(s) 46, 149, 866, 1121, 1751, 1854, 2075
BmsI GCATC 9 cut(s) 607, 726, 897, 1217, 1338, 1477, 1682, 1876, 1969
BoxI GACNNNNGTC 1 cut(s) 99
BpiI GAAGAC 4 cut(s) 294, 348, 635, 704
Bpu10I CCTNAGC 1 cut(s) 1890
Bpu14I TTCGAA 1 cut(s) 376
BpuEI CTTGAG 1 cut(s) 2044
BpuMI CCSGG 2 cut(s) 149, 2075
BsaAI YACGTR 1 cut(s) 1604
BsaBI GATNNNNATC 1 cut(s) 204
BsaJI CCNNGG 5 cut(s) 45, 1120, 1829, 1852, 2073
BsaWI WCCGGW 1 cut(s) 1439
BsaXI ACNNNNNCTCC 3 cut(s) 28, 1410, 1440
Bsc4I CCNNNNNNNGG 4 cut(s) 1096, 1483, 1570, 1835
Bse118I RCCGGY 1 cut(s) 2183
Bse1I ACTGG 1 cut(s) 1543
Bse21I CCTNAGG 1 cut(s) 687
Bse3DI GCAATG 1 cut(s) 2012
Bse8I GATNNNNATC 1 cut(s) 204
BseBI CCWGG 5 cut(s) 46, 866, 1121, 1751, 1854
BseDI CCNNGG 5 cut(s) 45, 1120, 1829, 1852, 2073
BseJI GATNNNNATC 1 cut(s) 204
BseLI CCNNNNNNNGG 4 cut(s) 1096, 1483, 1570, 1835
BseMI GCAATG 1 cut(s) 2012
BseMII CTCAG 9 cut(s) 496, 678, 689, 1013, 1071, 1223, 1482, 1698, 1904
BseNI ACTGG 1 cut(s) 1543
BseSI GKGCMC 1 cut(s) 550
BseXI GCAGC 2 cut(s) 679, 1436
BseYI CCCAGC 1 cut(s) 2187
BsgI GTGCAG 3 cut(s) 99, 678, 1094
Bsh1236I CGCG 3 cut(s) 495, 1377, 1823
BshFI GGCC 2 cut(s) 2038, 2166
BshNI GGYRCC 1 cut(s) 2077
BsiHKAI GWGCWC 2 cut(s) 550, 1948
BsiSI CCGG 4 cut(s) 149, 1440, 2075, 2184
BslFI GGGAC 6 cut(s) 458, 1137, 1789, 2049, 2054, 2204
BslI CCNNNNNNNGG 4 cut(s) 1096, 1483, 1570, 1835
BsmAI GTCTC 1 cut(s) 496
BsmFI GGGAC 6 cut(s) 458, 1137, 1789, 2049, 2054, 2204
BsmI GAATGC 1 cut(s) 2131
BsnI GGCC 2 cut(s) 2038, 2166
Bsp119I TTCGAA 1 cut(s) 376
Bsp1286I GDGCHC 3 cut(s) 550, 704, 1948
Bsp1407I TGTACA 1 cut(s) 347
Bsp68I TCGCGA 2 cut(s) 1377, 1823
BspACI CCGC 5 cut(s) 169, 493, 769, 1523, 1916
BspANI GGCC 2 cut(s) 2038, 2166
BspCNI CTCAG 9 cut(s) 495, 679, 690, 1012, 1070, 1224, 1483, 1697, 1903
BspFNI CGCG 3 cut(s) 495, 1377, 1823
BspHI TCATGA 1 cut(s) 160
BspLI GGNNCC 3 cut(s) 179, 2079, 2165
BspMAI CTGCAG 1 cut(s) 101
BspPI GGATC 4 cut(s) 36, 944, 1054, 1152
BspT104I TTCGAA 1 cut(s) 376
BspT107I GGYRCC 1 cut(s) 2077
BsrDI GCAATG 1 cut(s) 2012
BsrFI RCCGGY 1 cut(s) 2183
BsrGI TGTACA 1 cut(s) 347
BsrI ACTGG 1 cut(s) 1543
BssAI RCCGGY 1 cut(s) 2183
BssECI CCNNGG 5 cut(s) 45, 1120, 1829, 1852, 2073
BssSI CACGAG 1 cut(s) 2168
BssT1I CCWWGG 1 cut(s) 1829
Bst2BI CACGAG 1 cut(s) 2168
Bst2UI CCWGG 5 cut(s) 46, 866, 1121, 1751, 1854
Bst4CI ACNGT 6 cut(s) 14, 293, 352, 545, 1130, 1198
Bst6I CTCTTC 5 cut(s) 12, 318, 1768, 1972, 2037
BstAPI GCANNNNNTGC 1 cut(s) 580
BstAUI TGTACA 1 cut(s) 347
BstBAI YACGTR 1 cut(s) 1604
BstBI TTCGAA 1 cut(s) 376
BstC8I GCNNGC 2 cut(s) 1544, 2185
BstEII GGTNACC 2 cut(s) 1579, 2223
BstFNI CGCG 3 cut(s) 495, 1377, 1823
BstH2I RGCGCY 1 cut(s) 169
BstHHI GCGC 1 cut(s) 168
BstMAI GTCTC 1 cut(s) 496
BstMWI GCNNNNNNNGC 5 cut(s) 483, 492, 580, 1506, 1566
BstNI CCWGG 5 cut(s) 46, 866, 1121, 1751, 1854
BstNSI RCATGY 2 cut(s) 1266, 2143
BstPAI GACNNNNGTC 1 cut(s) 99
BstPI GGTNACC 2 cut(s) 1579, 2223
BstSCI CCNGG 7 cut(s) 44, 147, 864, 1119, 1749, 1852, 2073
BstSFI CTRYAG 1 cut(s) 97
BstSLI GKGCMC 1 cut(s) 550
BstUI CGCG 3 cut(s) 495, 1377, 1823
BstV1I GCAGC 2 cut(s) 679, 1436
BstV2I GAAGAC 4 cut(s) 294, 348, 635, 704
BstX2I RGATCY 1 cut(s) 1144
BstXI CCANNNNNNTGG 1 cut(s) 2219
BstYI RGATCY 1 cut(s) 1144
Bsu36I CCTNAGG 1 cut(s) 687
BsuRI GGCC 2 cut(s) 2038, 2166
BtrI CACGTC 1 cut(s) 1758
BtsI GCAGTG 3 cut(s) 667, 1386, 2178
BtuMI TCGCGA 2 cut(s) 1377, 1823
Cac8I GCNNGC 2 cut(s) 1544, 2185
CciI TCATGA 1 cut(s) 160
CfoI GCGC 1 cut(s) 168
Cfr10I RCCGGY 1 cut(s) 2183
Cfr13I GGNCC 2 cut(s) 52, 2164
Csp6I GTAC 4 cut(s) 348, 607, 1601, 2110
CviAII CATG 5 cut(s) 161, 1152, 1263, 1664, 2140
CviQI GTAC 4 cut(s) 348, 607, 1601, 2110
Eam1104I CTCTTC 5 cut(s) 12, 318, 1768, 1972, 2037
EarI CTCTTC 5 cut(s) 12, 318, 1768, 1972, 2037
Eco130I CCWWGG 1 cut(s) 1829
Eco24I GRGCYC 1 cut(s) 704
Eco47I GGWCC 1 cut(s) 52
Eco57I CTGAAG 3 cut(s) 297, 1940, 2061
Eco81I CCTNAGG 1 cut(s) 687
Eco91I GGTNACC 2 cut(s) 1579, 2223
EcoO109I RGGNCCY 2 cut(s) 52, 2164
EcoO65I GGTNACC 2 cut(s) 1579, 2223
EcoRI GAATTC 1 cut(s) 1115
EcoRII CCWGG 5 cut(s) 44, 864, 1119, 1749, 1852
EcoT14I CCWWGG 1 cut(s) 1829
EcoT22I ATGCAT 2 cut(s) 1502, 1962
EcoT38I GRGCYC 1 cut(s) 704
ErhI CCWWGG 1 cut(s) 1829
FaeI CATG 5 cut(s) 164, 1155, 1266, 1667, 2143
FalI AAGNNNNNCTT 2 cut(s) 2034, 2066
FaqI GGGAC 6 cut(s) 458, 1137, 1789, 2049, 2054, 2204
FatI CATG 5 cut(s) 160, 1151, 1262, 1663, 2139
FauI CCCGC 1 cut(s) 1516
FauNDI CATATG 1 cut(s) 2157
Fnu4HI GCNGC 3 cut(s) 169, 693, 1450
FriOI GRGCYC 1 cut(s) 704
Fsp4HI GCNGC 3 cut(s) 169, 693, 1450
FspBI CTAG 3 cut(s) 386, 603, 2100
GlaI GCGC 1 cut(s) 167
GluI GCNGC 3 cut(s) 169, 693, 1450
GsaI CCCAGC 1 cut(s) 2191
HaeII RGCGCY 1 cut(s) 169
HaeIII GGCC 2 cut(s) 2038, 2166
HapII CCGG 4 cut(s) 149, 1440, 2075, 2184
HhaI GCGC 1 cut(s) 168
Hin1II CATG 5 cut(s) 164, 1155, 1266, 1667, 2143
Hin6I GCGC 1 cut(s) 166
HinP1I GCGC 1 cut(s) 166
HindIII AAGCTT 1 cut(s) 1922
HpaII CCGG 4 cut(s) 149, 1440, 2075, 2184
HphI GGTGA 7 cut(s) 479, 919, 1145, 1429, 1466, 1573, 1962
Hpy166II GTNNAC 6 cut(s) 348, 548, 1009, 1729, 1843, 2056
Hpy188I TCNGA 8 cut(s) 70, 210, 235, 1054, 1687, 2107, 2136, 2204
Hpy8I GTNNAC 6 cut(s) 348, 548, 1009, 1729, 1843, 2056
HpyAV CCTTC 4 cut(s) 435, 573, 898, 963
HpyCH4III ACNGT 6 cut(s) 14, 293, 352, 545, 1130, 1198
HpyCH4IV ACGT 2 cut(s) 1603, 1757
HpyF10VI GCNNNNNNNGC 5 cut(s) 483, 492, 580, 1506, 1566
HpySE526I ACGT 2 cut(s) 1603, 1757
Hsp92II CATG 5 cut(s) 164, 1155, 1266, 1667, 2143
HspAI GCGC 1 cut(s) 166
KroI GCCGGC 1 cut(s) 2183
KroNI GCCGGC 1 cut(s) 2185
LmnI GCTCC 1 cut(s) 771
Lsp1109I GCAGC 2 cut(s) 679, 1436
LweI GCATC 9 cut(s) 607, 726, 897, 1217, 1338, 1477, 1682, 1876, 1969
MaeI CTAG 3 cut(s) 386, 603, 2100
MaeII ACGT 2 cut(s) 1603, 1757
MaeIII GTNAC 9 cut(s) 8, 654, 1220, 1258, 1384, 1435, 1579, 2176, 2223
MfeI CAATTG 1 cut(s) 1311
MflI RGATCY 1 cut(s) 1144
MhlI GDGCHC 3 cut(s) 550, 704, 1948
MlyI GAGTC 4 cut(s) 753, 1049, 2018, 2224
MmeI TCCRAC 4 cut(s) 443, 564, 1986, 2054
Mph1103I ATGCAT 2 cut(s) 1502, 1962
MroNI GCCGGC 1 cut(s) 2183
MroXI GAANNNNTTC 2 cut(s) 156, 1341
MseI TTAA 4 cut(s) 647, 1094, 1638, 2239
MslI CAYNNNNRTG 3 cut(s) 1131, 1407, 2217
MspA1I CMGCKG 2 cut(s) 1523, 1918
MspI CCGG 4 cut(s) 149, 1440, 2075, 2184
MspR9I CCNGG 7 cut(s) 46, 149, 866, 1121, 1751, 1854, 2075
MunI CAATTG 1 cut(s) 1311
Mva1269I GAATGC 1 cut(s) 2131
MvaI CCWGG 5 cut(s) 46, 866, 1121, 1751, 1854
MvnI CGCG 3 cut(s) 495, 1377, 1823
MwoI GCNNNNNNNGC 5 cut(s) 483, 492, 580, 1506, 1566
NaeI GCCGGC 1 cut(s) 2185
NciI CCSGG 2 cut(s) 149, 2075
NdeI CATATG 1 cut(s) 2157
NgoMIV GCCGGC 1 cut(s) 2183
NlaIII CATG 5 cut(s) 164, 1155, 1266, 1667, 2143
NlaIV GGNNCC 3 cut(s) 179, 2079, 2165
NmuCI GTSAC 6 cut(s) 1220, 1258, 1384, 1435, 1579, 2176
NruI TCGCGA 2 cut(s) 1377, 1823
NsiI ATGCAT 2 cut(s) 1502, 1962
NspI RCATGY 2 cut(s) 1266, 2143
NspV TTCGAA 1 cut(s) 376
OliI CACNNNNGTG 1 cut(s) 1131
PagI TCATGA 1 cut(s) 160
PciI ACATGT 1 cut(s) 1262
PcsI WCGNNNNNNNCGW 2 cut(s) 119, 1820
PctI GAATGC 1 cut(s) 2131
PdiI GCCGGC 1 cut(s) 2185
PdmI GAANNNNTTC 2 cut(s) 156, 1341
PfeI GAWTC 6 cut(s) 230, 489, 532, 976, 1592, 1718
PkrI GCNGC 3 cut(s) 170, 694, 1451
PleI GAGTC 4 cut(s) 752, 1049, 2017, 2224
PpsI GAGTC 4 cut(s) 752, 1049, 2017, 2224
Ppu21I YACGTR 1 cut(s) 1604
PpuMI RGGWCCY 1 cut(s) 52
PscI ACATGT 1 cut(s) 1262
PshAI GACNNNNGTC 1 cut(s) 99
PsiI TTATAA 1 cut(s) 1458
Psp5II RGGWCCY 1 cut(s) 52
Psp6I CCWGG 5 cut(s) 44, 864, 1119, 1749, 1852
PspEI GGTNACC 2 cut(s) 1579, 2223
PspFI CCCAGC 1 cut(s) 2187
PspGI CCWGG 5 cut(s) 44, 864, 1119, 1749, 1852
PspN4I GGNNCC 3 cut(s) 179, 2079, 2165
PspPI GGNCC 2 cut(s) 52, 2164
PspPPI RGGWCCY 1 cut(s) 52
PstI CTGCAG 1 cut(s) 101
PsuI RGATCY 1 cut(s) 1144
RruI TCGCGA 2 cut(s) 1377, 1823
RsaI GTAC 4 cut(s) 349, 608, 1602, 2111
RsaNI GTAC 4 cut(s) 348, 607, 1601, 2110
RseI CAYNNNNRTG 3 cut(s) 1131, 1407, 2217
SaqAI TTAA 4 cut(s) 647, 1094, 1638, 2239
SatI GCNGC 3 cut(s) 169, 693, 1450
Sau96I GGNCC 2 cut(s) 52, 2164
SchI GAGTC 4 cut(s) 753, 1049, 2018, 2224
ScrFI CCNGG 7 cut(s) 46, 149, 866, 1121, 1751, 1854, 2075
SduI GDGCHC 3 cut(s) 550, 704, 1948
SfaNI GCATC 9 cut(s) 607, 726, 897, 1217, 1338, 1477, 1682, 1876, 1969
SfcI CTRYAG 1 cut(s) 97
SfuI TTCGAA 1 cut(s) 376
SinI GGWCC 1 cut(s) 52
SmiMI CAYNNNNRTG 3 cut(s) 1131, 1407, 2217
SmlI CTYRAG 1 cut(s) 2059
SmoI CTYRAG 1 cut(s) 2059
SsiI CCGC 5 cut(s) 169, 493, 769, 1523, 1916
SspMI CTAG 3 cut(s) 386, 603, 2100
StyD4I CCNGG 7 cut(s) 44, 147, 864, 1119, 1749, 1852, 2073
StyI CCWWGG 1 cut(s) 1829
TaaI ACNGT 6 cut(s) 14, 293, 352, 545, 1130, 1198
TaiI ACGT 2 cut(s) 1606, 1760
TaqI TCGA 7 cut(s) 122, 376, 439, 836, 1201, 1814, 1935
TatI WGTACW 2 cut(s) 347, 2109
TauI GCSGC 1 cut(s) 171
TfiI GAWTC 6 cut(s) 230, 489, 532, 976, 1592, 1718
Tru1I TTAA 4 cut(s) 647, 1094, 1638, 2239
Tru9I TTAA 4 cut(s) 647, 1094, 1638, 2239
TseFI GTSAC 6 cut(s) 1220, 1258, 1384, 1435, 1579, 2176
TseI GCWGC 2 cut(s) 692, 1449
Tsp45I GTSAC 6 cut(s) 1220, 1258, 1384, 1435, 1579, 2176
TspDTI ATGAA 9 cut(s) 149, 296, 351, 474, 524, 854, 989, 1288, 1867
TspGWI ACGGA 4 cut(s) 742, 884, 1079, 1332
VneI GTGCAC 1 cut(s) 546
VpaK11BI GGWCC 1 cut(s) 52
XapI RAATTY 7 cut(s) 211, 406, 413, 805, 932, 1115, 1634
XbaI TCTAGA 1 cut(s) 2099
XceI RCATGY 2 cut(s) 1266, 2143
XcmI CCANNNNNNNNNTGG 1 cut(s) 1127
XmnI GAANNNNTTC 2 cut(s) 156, 1341
XspI CTAG 3 cut(s) 386, 603, 2100
Zsp2I ATGCAT 2 cut(s) 1502, 1962
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.