Rh7AG201800

Scarecrow-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0002481
Physical Location & Seq
Forward (+)
18361833 .. 18365601
3769 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG201800.1

Sequence Viewer

Length: 2343 bp
ATGCCCTTTCAATTTCAGCAACAGGGGAAGGGGGTCTTAGAAATTACAGCGGCATCAATCTGTGACTGGAACCACAAGAAAGACGAGGAGCTTTTCTTCAACAACAGCTTCGTCAGCAACGAACCCACCTCTGTTCTTCATATGAGAAGGAGCCCGAGCCCACCCACTTCGGCATCCACTCTCTCCTCCTCCTTCAACGGCGGCGGCAACGGCAACGGCAACACCACCACGCCGCTTCTGCAGGACACTTCGCCGGTGGGACCCGAGCCCGACACGGCCGCGGCGCGCAGCAGCAGCAAGGATGAGTGGACCTGCGAGCTTCAGTCGATTCCCAGCGGCGGTCTGGAGGCCGTGACCGGATCGGGCGGCGGGAGATGCGGCGGGCTTGGGTTGGGGGACTGGGAGACCGTGCTGTCCGAAACGGCGGCGTCTCCGGGTCAAGACCAGTCGCTGCTCCGGTGGATCTCGGGCGACGTGGACGACGCGTCGTTTGGGCTAAAGCAGCTGTTGCAGAGCGGGAACAACAGCAACTGTCTTGAGTTTGACGGCAATGCGGGTCTGGGAATGGTCGATCAGGGTTCCGGTTTCGACCACATCGGGAGCAGCGTTTCGGGTCCCAATTTGGGGTTCTCCGGCGGTTCTGGTTTCAATGGGAAAATGGGTTTTGTGCCCAGCTCTTCTGCGCCGCTGAATTACAAGGTTTCAAATGTTGGGTTGATGAACAATAATGGCGGCAACAACAACAACTTCGCCAATTCAGCACTTCCAGTTTCGCTGCCTCATGGAATGGTTTATCAGAATCACCAGCAGTTTGAGAACCCAGATGAGAAGCCTCAGATTCTGAACCCACAGATGTTCATGAACCAGCACCAGTCTCAGCTCCCTCATCAAAATCCAAACTTTTTCATGCCAGTGACTTATGCTCAGCAGGAGCAGCACTTTCTTCAGTCCCAGCCCAAGCGCCACAACCCAGGTGGCGGCGGTGGAGGACTGGACCCGAGTTCTCAGATCCACCAAAAGGTACAATATTCTGATCCGGGTCATGAGTTTCTGCTCAGAAAGCAACACCTTCAGCAGCAGCAGCAGCAGCCCCATTTTGGTAATTTCCCTCCCGGAATGCAGTTCCTTCCTCAGCAGCACCACACTCAACCGAGGCCGGTAGTGGTGCCCAAGCAGAAGGTGGGTGGCGAAGAAATGGCGGCGGCGGCTCACCAGAACCAGCAGCAACTGCTGCAGCATAGTTTGCTGGACCAGCTCTATAAGGCCGCAGAAATGGTAGGTACTGGGAACTTCTCACACGCGCAGGCGATATTGGCGCGGCTCAATCACCAGCTCTCCCCTGTTGGAAAGCCCCTTCAGCGGGCTGCTTTCTATTTCAAGGAGGCTTTGCAACTCCTCCTCCTTATGAACAACCCAGCAACCTCTCCTCCACCAAGAACCCCGACCCCTTTTGATGTCATCTTCAAAATGGGGGCTTACAAAGTCTTCTCTGAAGTGTCTCCATTGATTCAGTTTGTCAATTTTACTTGCAACCAGGCTCTACTTGAGGCTGTATCAGAAGCTGATCAGATTCACATTGTTGATTTTGATATTGGGTTTGGTGCTCACTGGGCCTCCTTTATGCAGGAGCTCCCATTGAGGAACAGGGGTGGCGCTTCCCCCTCTCTCAAAATCACTGCTTTTGCCTCCCCTTCTACTCATCACACAGTTGAGCTTAATCTTATGCGTGATAATTTAACCCAATTTGCTGATGAGATTGGCTTAGGTTTTGAGCTTGAAGTAGTTAACATTGATTTGTTGGACCAAAACTCATACTCACTGCCCTTTTTCCGAGCTAATGATAATGAGGCCGTTGCTGTGAATTTCCCCATTTGGTCTAGTTCGAATCAACCGGCTGCTTTGCCCAATCTCCTTCAATTTGTTAAACAGCTCTCTCCCAAAATTGTGGTGTCTTTGGATAGAGGGTGTGACAGGAGTGACCTTCCATTCCCACAGCACATTCGGCAGGCCCTCCAATCCTACATAAACCTCTTGGAATCTCTTGATGCGGTTAATGTAACTTCGGATGCTGTGAACAAGATTGAGAGGTTCCTCCTTCAGCCTAAGATTGAAAGCACTGTTTTGGGGCGTCTCCGAACCCCTGACAAAATGCCCCTATGGAAAACGCTCTTTGCCTCAGCTGGTTTCACTCCATTATCCTTCAGTAACTTCACGGAGACTCAGGCAGAATGTGTGGTCAAGAGGAGTACAGCAAGAGGGTTTCATGTCGAGAAGCGTCAAGAGTCACTTGTGCTATGCTGGCAGCGCCGAGAGCTCATCTCAGCTTCAGCTTGGAGGTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

780

Amino Acids

85.46

Weight (kDa)

5.97

Isoelectric Point (pI)

55.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 416 - 779 1.8e-93 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 412
Acc36I ACCTGC 1 cut(s) 320
AccB1I GGYRCC 1 cut(s) 1165
AccBSI CCGCTC 1 cut(s) 516
AccII CGCG 5 cut(s) 281, 286, 485, 1301, 1318
AclWI GGATC 4 cut(s) 367, 470, 1003, 1028
AcoI YGGCCR 1 cut(s) 276
AcsI RAATTY 1 cut(s) 1861
AcuI CTGAAG 8 cut(s) 305, 929, 1055, 1340, 1512, 2081, 2185, 2310
AcyI GRCGYC 2 cut(s) 428, 2128
AfaI GTAC 3 cut(s) 1023, 1282, 2248
AfiI CCNNNNNNNGG 8 cut(s) 338, 623, 624, 977, 1018, 1097, 1359, 1360
AflIII ACRYGT 1 cut(s) 483
AhdI GACNNNNNGTC 1 cut(s) 484
AjiI CACGTC 1 cut(s) 475
AjnI CCWGG 2 cut(s) 970, 1533
AjuI GAANNNNNNNTTGG 2 cut(s) 611, 643
Alw21I GWGCWC 3 cut(s) 1606, 1632, 2316
Alw26I GTCTC 6 cut(s) 398, 435, 879, 1503, 2135, 2210
AlwI GGATC 4 cut(s) 367, 470, 1003, 1028
AlwNI CAGNNNCTG 5 cut(s) 451, 531, 841, 1228, 1562
Ama87I CYCGRG 4 cut(s) 154, 263, 466, 997
AoxI GGCC 7 cut(s) 276, 348, 1154, 1263, 1611, 1848, 2007
ApoI RAATTY 1 cut(s) 1861
ArsI GACNNNNNNTTYG 2 cut(s) 473, 505
AspLEI GCGC 8 cut(s) 286, 288, 685, 963, 1303, 1318, 1655, 2307
AspS9I GGNCC 8 cut(s) 260, 309, 614, 994, 1249, 1611, 1801, 2008
AsuC2I CCSGG 3 cut(s) 435, 1038, 1113
AsuHPI GGTGA 3 cut(s) 794, 1202, 1319
AsuII TTCGAA 1 cut(s) 1883
AvaI CYCGRG 4 cut(s) 154, 263, 466, 997
AvaII GGWCC 6 cut(s) 260, 309, 614, 994, 1249, 1801
BaeGI GKGCMC 2 cut(s) 672, 1170
BanI GGYRCC 1 cut(s) 1165
BanII GRGCYC 5 cut(s) 155, 161, 270, 1632, 2316
BbsI GAAGAC 1 cut(s) 1477
Bbv12I GWGCWC 3 cut(s) 1606, 1632, 2316
BbvCI CCTCAGC 2 cut(s) 1131, 2176
BceAI ACGGC 8 cut(s) 214, 226, 232, 291, 335, 438, 562, 1835
BciT130I CCWGG 2 cut(s) 972, 1535
BclI TGATCA 1 cut(s) 1564
BcnI CCSGG 3 cut(s) 435, 1038, 1113
BcoDI GTCTC 6 cut(s) 398, 435, 879, 1503, 2135, 2210
BfaI CTAG 1 cut(s) 1878
BfmI CTRYAG 2 cut(s) 239, 1232
BfoI RGCGCY 3 cut(s) 964, 1656, 2308
BfuAI ACCTGC 1 cut(s) 320
BlpI GCTNAGC 1 cut(s) 924
Bme1390I CCNGG 5 cut(s) 435, 972, 1038, 1113, 1535
Bme18I GGWCC 6 cut(s) 260, 309, 614, 994, 1249, 1801
BmeRI GACNNNNNGTC 1 cut(s) 484
BmeT110I CYCGRG 4 cut(s) 154, 263, 466, 997
BmgBI CACGTC 1 cut(s) 475
BmgT120I GGNCC 8 cut(s) 260, 309, 614, 994, 1249, 1611, 1801, 2008
BmrFI CCNGG 5 cut(s) 435, 972, 1038, 1113, 1535
BmrI ACTGGG 3 cut(s) 409, 1293, 1618
BmsI GCATC 5 cut(s) 62, 182, 365, 2035, 2056
BmuI ACTGGG 3 cut(s) 409, 1293, 1618
BpiI GAAGAC 1 cut(s) 1477
BplI GAGNNNNNCTC 2 cut(s) 2303, 2335
BpmI CTGGAG 1 cut(s) 365
Bpu10I CCTNAGC 3 cut(s) 1131, 1762, 2176
Bpu1102I GCTNAGC 1 cut(s) 924
Bpu14I TTCGAA 1 cut(s) 1883
BpuEI CTTGAG 2 cut(s) 557, 1565
BpuMI CCSGG 3 cut(s) 435, 1038, 1113
BsaHI GRCGYC 2 cut(s) 428, 2128
BsaI GGTCTC 1 cut(s) 398
BsaJI CCNNGG 3 cut(s) 279, 970, 1151
BsaWI WCCGGW 3 cut(s) 356, 456, 581
BsaXI ACNNNNNCTCC 8 cut(s) 1319, 1349, 1383, 1411, 1413, 1441, 1598, 1628
Bsc4I CCNNNNNNNGG 8 cut(s) 338, 623, 624, 977, 1018, 1097, 1359, 1360
Bse118I RCCGGY 3 cut(s) 253, 1156, 1891
Bse1I ACTGG 9 cut(s) 71, 404, 445, 767, 871, 911, 996, 1288, 1613
Bse3DI GCAATG 1 cut(s) 556
BseBI CCWGG 2 cut(s) 972, 1535
BseDI CCNNGG 3 cut(s) 279, 970, 1151
BseGI GGATG 3 cut(s) 173, 307, 2071
BseLI CCNNNNNNNGG 8 cut(s) 338, 623, 624, 977, 1018, 1097, 1359, 1360
BseMI GCAATG 1 cut(s) 556
BseMII CTCAG 9 cut(s) 848, 890, 938, 1019, 1069, 1145, 2190, 2234, 2334
BseNI ACTGG 9 cut(s) 71, 404, 445, 767, 871, 911, 996, 1288, 1613
BsePI GCGCGC 1 cut(s) 284
BseRI GAGGAG 7 cut(s) 101, 175, 178, 1385, 1388, 1416, 2257
BseSI GKGCMC 2 cut(s) 672, 1170
BseX3I CGGCCG 1 cut(s) 276
BseYI CCCAGC 4 cut(s) 332, 671, 951, 1414
Bsh1236I CGCG 5 cut(s) 281, 286, 485, 1301, 1318
Bsh1285I CGRYCG 1 cut(s) 279
BshFI GGCC 7 cut(s) 278, 350, 1156, 1265, 1613, 1850, 2009
BshNI GGYRCC 1 cut(s) 1165
BsiEI CGRYCG 1 cut(s) 279
BsiHKAI GWGCWC 3 cut(s) 1606, 1632, 2316
BsiHKCI CYCGRG 4 cut(s) 154, 263, 466, 997
BslFI GGGAC 4 cut(s) 273, 410, 600, 934
BslI CCNNNNNNNGG 8 cut(s) 338, 623, 624, 977, 1018, 1097, 1359, 1360
BsmAI GTCTC 6 cut(s) 398, 435, 879, 1503, 2135, 2210
BsmBI CGTCTC 2 cut(s) 435, 2135
BsmFI GGGAC 4 cut(s) 273, 410, 600, 934
BsmI GAATGC 1 cut(s) 1122
BsnI GGCC 7 cut(s) 278, 350, 1156, 1265, 1613, 1850, 2009
Bso31I GGTCTC 1 cut(s) 398
BsoBI CYCGRG 4 cut(s) 154, 263, 466, 997
Bsp119I TTCGAA 1 cut(s) 1883
Bsp1286I GDGCHC 8 cut(s) 155, 161, 270, 672, 1170, 1606, 1632, 2316
Bsp143I GATC 6 cut(s) 359, 462, 571, 1008, 1033, 1564
Bsp1720I GCTNAGC 1 cut(s) 924
BspANI GGCC 7 cut(s) 278, 350, 1156, 1265, 1613, 1850, 2009
BspCNI CTCAG 9 cut(s) 847, 889, 937, 1018, 1068, 1144, 2189, 2233, 2333
BspFNI CGCG 5 cut(s) 281, 286, 485, 1301, 1318
BspHI TCATGA 2 cut(s) 858, 1042
BspMAI CTGCAG 2 cut(s) 243, 1236
BspMI ACCTGC 1 cut(s) 320
BspPI GGATC 4 cut(s) 367, 470, 1003, 1028
BspQI GCTCTTC 1 cut(s) 682
BspT104I TTCGAA 1 cut(s) 1883
BspT107I GGYRCC 1 cut(s) 1165
BspTNI GGTCTC 1 cut(s) 398
BsrBI CCGCTC 1 cut(s) 516
BsrDI GCAATG 1 cut(s) 556
BsrFI RCCGGY 3 cut(s) 253, 1156, 1891
BsrI ACTGG 9 cut(s) 71, 404, 445, 767, 871, 911, 996, 1288, 1613
BssAI RCCGGY 3 cut(s) 253, 1156, 1891
BssECI CCNNGG 3 cut(s) 279, 970, 1151
BssHII GCGCGC 1 cut(s) 284
BssMI GATC 6 cut(s) 359, 462, 571, 1008, 1033, 1564
BssNI GRCGYC 2 cut(s) 428, 2128
Bst2UI CCWGG 2 cut(s) 972, 1535
Bst4CI ACNGT 4 cut(s) 409, 533, 1708, 2119
Bst6I CTCTTC 1 cut(s) 682
BstACI GRCGYC 2 cut(s) 428, 2128
BstAPI GCANNNNNTGC 4 cut(s) 508, 1228, 1231, 1243
BstBI TTCGAA 1 cut(s) 1883
BstC8I GCNNGC 7 cut(s) 286, 317, 383, 1305, 1362, 2007, 2300
BstDSI CCRYGG 1 cut(s) 279
BstF5I GGATG 3 cut(s) 173, 307, 2071
BstFNI CGCG 5 cut(s) 281, 286, 485, 1301, 1318
BstH2I RGCGCY 3 cut(s) 964, 1656, 2308
BstHHI GCGC 8 cut(s) 286, 288, 685, 963, 1303, 1318, 1655, 2307
BstKTI GATC 6 cut(s) 362, 465, 574, 1011, 1036, 1567
BstMAI GTCTC 6 cut(s) 398, 435, 879, 1503, 2135, 2210
BstMBI GATC 6 cut(s) 359, 462, 571, 1008, 1033, 1564
BstMCI CGRYCG 1 cut(s) 279
BstNI CCWGG 2 cut(s) 972, 1535
BstSCI CCNGG 5 cut(s) 433, 970, 1036, 1111, 1533
BstSFI CTRYAG 2 cut(s) 239, 1232
BstSLI GKGCMC 2 cut(s) 672, 1170
BstUI CGCG 5 cut(s) 281, 286, 485, 1301, 1318
BstV2I GAAGAC 1 cut(s) 1477
BstX2I RGATCY 2 cut(s) 462, 1008
BstXI CCANNNNNNTGG 1 cut(s) 1945
BstYI RGATCY 2 cut(s) 462, 1008
BstZI CGGCCG 1 cut(s) 276
BsuRI GGCC 7 cut(s) 278, 350, 1156, 1265, 1613, 1850, 2009
BtgI CCRYGG 1 cut(s) 279
BtrI CACGTC 1 cut(s) 475
BtsCI GGATG 3 cut(s) 173, 307, 2071
BtsI GCAGTG 2 cut(s) 1674, 1817
BtsIMutI CAGTG 5 cut(s) 918, 1606, 1674, 1817, 2115
BveI ACCTGC 1 cut(s) 320
Cac8I GCNNGC 7 cut(s) 286, 317, 383, 1305, 1362, 2007, 2300
CaiI CAGNNNCTG 5 cut(s) 451, 531, 841, 1228, 1562
CciI TCATGA 2 cut(s) 858, 1042
CfoI GCGC 8 cut(s) 286, 288, 685, 963, 1303, 1318, 1655, 2307
Cfr10I RCCGGY 3 cut(s) 253, 1156, 1891
Cfr13I GGNCC 8 cut(s) 260, 309, 614, 994, 1249, 1611, 1801, 2008
Cfr42I CCGCGG 1 cut(s) 282
CseI GACGC 5 cut(s) 417, 474, 491, 2117, 2264
Csp6I GTAC 3 cut(s) 1022, 1281, 2247
CspCI CAANNNNNGTGG 2 cut(s) 955, 990
CviAII CATG 5 cut(s) 782, 859, 907, 1043, 2264
CviQI GTAC 3 cut(s) 1022, 1281, 2247
DpnI GATC 6 cut(s) 361, 464, 573, 1010, 1035, 1566
DpnII GATC 6 cut(s) 359, 462, 571, 1008, 1033, 1564
DrdI GACNNNNNNGTC 1 cut(s) 412
DriI GACNNNNNGTC 1 cut(s) 484
DseDI GACNNNNNNGTC 1 cut(s) 412
EaeI YGGCCR 1 cut(s) 276
EagI CGGCCG 1 cut(s) 276
Eam1104I CTCTTC 1 cut(s) 682
Eam1105I GACNNNNNGTC 1 cut(s) 484
EarI CTCTTC 1 cut(s) 682
Ecl136II GAGCTC 2 cut(s) 1630, 2314
EclXI CGGCCG 1 cut(s) 276
Eco24I GRGCYC 5 cut(s) 155, 161, 270, 1632, 2316
Eco31I GGTCTC 1 cut(s) 398
Eco47I GGWCC 6 cut(s) 260, 309, 614, 994, 1249, 1801
Eco52I CGGCCG 1 cut(s) 276
Eco53kI GAGCTC 2 cut(s) 1630, 2314
Eco57I CTGAAG 8 cut(s) 305, 929, 1055, 1340, 1512, 2081, 2185, 2310
Eco88I CYCGRG 4 cut(s) 154, 263, 466, 997
EcoICRI GAGCTC 2 cut(s) 1630, 2314
EcoO109I RGGNCCY 3 cut(s) 260, 614, 2008
EcoRII CCWGG 2 cut(s) 970, 1533
EcoT38I GRGCYC 5 cut(s) 155, 161, 270, 1632, 2316
Esp3I CGTCTC 2 cut(s) 435, 2135
FaeI CATG 5 cut(s) 785, 862, 910, 1046, 2267
FalI AAGNNNNNCTT 4 cut(s) 20, 52, 2271, 2303
FaqI GGGAC 4 cut(s) 273, 410, 600, 934
FatI CATG 5 cut(s) 781, 858, 906, 1042, 2263
FauI CCCGC 5 cut(s) 362, 374, 509, 547, 1353
FauNDI CATATG 1 cut(s) 141
FbaI TGATCA 1 cut(s) 1564
FokI GGATG 3 cut(s) 160, 314, 2078
FriOI GRGCYC 5 cut(s) 155, 161, 270, 1632, 2316
FspBI CTAG 1 cut(s) 1878
GlaI GCGC 8 cut(s) 285, 287, 684, 962, 1302, 1317, 1654, 2306
GsaI CCCAGC 4 cut(s) 336, 675, 955, 1418
GsuI CTGGAG 1 cut(s) 365
HaeII RGCGCY 3 cut(s) 964, 1656, 2308
HaeIII GGCC 7 cut(s) 278, 350, 1156, 1265, 1613, 1850, 2009
HgaI GACGC 5 cut(s) 417, 474, 491, 2117, 2264
HhaI GCGC 8 cut(s) 286, 288, 685, 963, 1303, 1318, 1655, 2307
Hin1I GRCGYC 2 cut(s) 428, 2128
Hin1II CATG 5 cut(s) 785, 862, 910, 1046, 2267
Hin6I GCGC 8 cut(s) 284, 286, 683, 961, 1301, 1316, 1653, 2305
HinP1I GCGC 8 cut(s) 284, 286, 683, 961, 1301, 1316, 1653, 2305
HincII GTYRAC 1 cut(s) 1786
HindII GTYRAC 1 cut(s) 1786
HinfI GANTC 9 cut(s) 328, 799, 838, 1507, 1570, 1885, 2036, 2218, 2282
HpaI GTTAAC 1 cut(s) 1786
HphI GGTGA 3 cut(s) 794, 1202, 1319
Hpy166II GTNNAC 4 cut(s) 309, 478, 1786, 2074
Hpy8I GTNNAC 4 cut(s) 309, 478, 1786, 2074
Hpy99I CGWCG 3 cut(s) 476, 485, 490
HpyCH4III ACNGT 4 cut(s) 409, 533, 1708, 2119
HpyCH4IV ACGT 1 cut(s) 474
HpyCH4V TGCA 7 cut(s) 241, 511, 1120, 1234, 1390, 1530, 1624
HpySE526I ACGT 1 cut(s) 474
Hsp92I GRCGYC 2 cut(s) 428, 2128
Hsp92II CATG 5 cut(s) 785, 862, 910, 1046, 2267
HspAI GCGC 8 cut(s) 284, 286, 683, 961, 1301, 1316, 1653, 2305
KflI GGGWCCC 2 cut(s) 260, 614
Ksp22I TGATCA 1 cut(s) 1564
KspAI GTTAAC 1 cut(s) 1786
KspI CCGCGG 1 cut(s) 282
Kzo9I GATC 6 cut(s) 359, 462, 571, 1008, 1033, 1564
LguI GCTCTTC 1 cut(s) 682
LmnI GCTCC 8 cut(s) 88, 150, 459, 600, 885, 931, 1627, 1635
LweI GCATC 5 cut(s) 62, 182, 365, 2035, 2056
MaeI CTAG 1 cut(s) 1878
MaeII ACGT 1 cut(s) 474
MaeIII GTNAC 8 cut(s) 62, 352, 913, 1967, 1976, 2056, 2204, 2283
MalI GATC 6 cut(s) 361, 464, 573, 1010, 1035, 1566
MbiI CCGCTC 1 cut(s) 516
MboI GATC 6 cut(s) 359, 462, 571, 1008, 1033, 1564
MboII GAAGA 7 cut(s) 88, 128, 669, 935, 1202, 1453, 1477
MflI RGATCY 2 cut(s) 462, 1008
MhlI GDGCHC 8 cut(s) 155, 161, 270, 672, 1170, 1606, 1632, 2316
MluI ACGCGT 1 cut(s) 483
MlyI GAGTC 2 cut(s) 2212, 2291
MmeI TCCRAC 2 cut(s) 1324, 1779
MseI TTAA 5 cut(s) 1716, 1736, 1785, 1923, 2052
MslI CAYNNNNRTG 1 cut(s) 911
MspA1I CMGCKG 7 cut(s) 50, 281, 336, 505, 688, 1360, 2180
MspR9I CCNGG 5 cut(s) 435, 972, 1038, 1113, 1535
Mva1269I GAATGC 1 cut(s) 1122
MvaI CCWGG 2 cut(s) 972, 1535
MvnI CGCG 5 cut(s) 281, 286, 485, 1301, 1318
NciI CCSGG 3 cut(s) 435, 1038, 1113
NdeI CATATG 1 cut(s) 141
NdeII GATC 6 cut(s) 359, 462, 571, 1008, 1033, 1564
NlaIII CATG 5 cut(s) 785, 862, 910, 1046, 2267
NmeAIII GCCGAG 1 cut(s) 2333
NmuCI GTSAC 6 cut(s) 62, 352, 913, 1967, 1976, 2283
NspV TTCGAA 1 cut(s) 1883
PagI TCATGA 2 cut(s) 858, 1042
PauI GCGCGC 1 cut(s) 284
PciSI GCTCTTC 1 cut(s) 682
PcsI WCGNNNNNNNCGW 2 cut(s) 117, 603
PctI GAATGC 1 cut(s) 1122
PfeI GAWTC 7 cut(s) 328, 799, 838, 1507, 1570, 1885, 2036
PfoI TCCNGGA 1 cut(s) 1111
PleI GAGTC 2 cut(s) 2212, 2290
PpsI GAGTC 2 cut(s) 2212, 2290
PpuMI RGGWCCY 2 cut(s) 260, 614
Psp124BI GAGCTC 2 cut(s) 1632, 2316
Psp5II RGGWCCY 2 cut(s) 260, 614
Psp6I CCWGG 2 cut(s) 970, 1533
PspFI CCCAGC 4 cut(s) 332, 671, 951, 1414
PspGI CCWGG 2 cut(s) 970, 1533
PspPI GGNCC 8 cut(s) 260, 309, 614, 994, 1249, 1611, 1801, 2008
PspPPI RGGWCCY 2 cut(s) 260, 614
PstI CTGCAG 2 cut(s) 243, 1236
PstNI CAGNNNCTG 5 cut(s) 451, 531, 841, 1228, 1562
PsuI RGATCY 2 cut(s) 462, 1008
PteI GCGCGC 1 cut(s) 284
PvuII CAGCTG 2 cut(s) 505, 2180
RsaI GTAC 3 cut(s) 1023, 1282, 2248
RsaNI GTAC 3 cut(s) 1022, 1281, 2247
RseI CAYNNNNRTG 1 cut(s) 911
SacI GAGCTC 2 cut(s) 1632, 2316
SacII CCGCGG 1 cut(s) 282
SapI GCTCTTC 1 cut(s) 682
SaqAI TTAA 5 cut(s) 1716, 1736, 1785, 1923, 2052
Sau3AI GATC 6 cut(s) 359, 462, 571, 1008, 1033, 1564
Sau96I GGNCC 8 cut(s) 260, 309, 614, 994, 1249, 1611, 1801, 2008
SchI GAGTC 2 cut(s) 2212, 2291
ScrFI CCNGG 5 cut(s) 435, 972, 1038, 1113, 1535
SduI GDGCHC 8 cut(s) 155, 161, 270, 672, 1170, 1606, 1632, 2316
SfaNI GCATC 5 cut(s) 62, 182, 365, 2035, 2056
SfcI CTRYAG 2 cut(s) 239, 1232
Sfr303I CCGCGG 1 cut(s) 282
SfuI TTCGAA 1 cut(s) 1883
SgrAI CRCCGGYG 1 cut(s) 253
SgrBI CCGCGG 1 cut(s) 282
SinI GGWCC 6 cut(s) 260, 309, 614, 994, 1249, 1801
SmiMI CAYNNNNRTG 1 cut(s) 911
SmlI CTYRAG 2 cut(s) 536, 1544
SmoI CTYRAG 2 cut(s) 536, 1544
SspI AATATT 1 cut(s) 1028
SspMI CTAG 1 cut(s) 1878
SstI GAGCTC 2 cut(s) 1632, 2316
StyD4I CCNGG 5 cut(s) 433, 970, 1036, 1111, 1533
TaaI ACNGT 4 cut(s) 409, 533, 1708, 2119
TaiI ACGT 1 cut(s) 477
TaqI TCGA 5 cut(s) 326, 570, 588, 1883, 2268
TatI WGTACW 1 cut(s) 2246
TfiI GAWTC 7 cut(s) 328, 799, 838, 1507, 1570, 1885, 2036
Tru1I TTAA 5 cut(s) 1716, 1736, 1785, 1923, 2052
Tru9I TTAA 5 cut(s) 1716, 1736, 1785, 1923, 2052
TscAI CASTG 5 cut(s) 918, 1613, 1681, 1824, 2122
TseFI GTSAC 6 cut(s) 62, 352, 913, 1967, 1976, 2283
Tsp45I GTSAC 6 cut(s) 62, 352, 913, 1967, 1976, 2283
TspDTI ATGAA 7 cut(s) 128, 734, 847, 875, 895, 1421, 2252
TspGWI ACGGA 1 cut(s) 2228
TspRI CASTG 5 cut(s) 918, 1613, 1681, 1824, 2122
VpaK11BI GGWCC 6 cut(s) 260, 309, 614, 994, 1249, 1801
XapI RAATTY 1 cut(s) 1861
XcmI CCANNNNNNNNNTGG 3 cut(s) 340, 971, 1177
XspI CTAG 1 cut(s) 1878
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.