MD00G1043300.v1.1

Scarecrow-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0002481
Physical Location & Seq
Reverse (-)
7806171 .. 7809643
3473 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1043300.v1.1.491

Sequence Viewer

Length: 2427 bp
ATGCCCTTTCAGTTCCAGCAGGGGAAGGGGGTCATCGAAATAGCAACTGCAAGTTTGCCGGCAATTTTCACAGACAGCAGCTGCAGCAAGTACAAAGACGGCGGATTTTTCAACCACCACCACCACAGCTTTCCGACCAGCGAGCCCAATTCGGTTCTTCATATGAGAAGAAGCCCGAGCCCACCCACTTCGGCTTCCACTCTCTCTTCCTCTTTCAACAACGGCGGCGGTGGCGGCGACAAATTGACGCCGCTGGCAGCTGAGACTCCGCCTGCGGTGGGACAAACGTCGTCGAAAGACGAGTGGGCCTGCGAGCTCCAGTCGATTCCGAGTGGGGGTTTGGAGGCCGTGACCGGATCAGCAGGAGGAGGAGTCGAGAGATGCGACCTTGGGCTTGGCGGGTTGGAGGATTGGGAGACCATGCTCTCCGAAACGACGGCGTCTCCGGGTCACGACCAGTCGCTGCTGAGGTGGATCGCTGGGGATGTGGACGACACGTCGTTTGGGCTCAAGCAGCTCTTGCAGAGCGGGAACAACCACCACCGCCACCACAACAGCCCCCTTGATTTCGACGGCAATGCCGGGTTGGGTGTTGTCGATCAGGGCCCTAATTTCGACCTAATTGGAAACGGCGGCGTTTCGGGCCCGAATTTCGGGTTCGGCGGTTCGGGTTTTGGGAACGGCAGTAACAATGGCAGCAATGGGAAGCTGGGTTTTGTTCCTCCGGGTTCTGGGTCTTTTGGGTTGCTAAATTACAAGGTTTCAAATGTTGAGTTGATTCACAACAATGGCGGCAGCAACAGTGTCAATTGCAACATTCAAAACCCCATTTTCTCGAATTCGTCTAACACTTTGGCTCTGCCGGTATCTTTGCCTGCGGTTTACGGGCAGCAGCAATTAAGCCCGGACGAGAAGCCGCAGATTCTGAACCCCCAGCTGTTCATGAGCAACCAAAATCAGCAGCCTCAGGTGGCTCAGAATCCGAACTTTTTCATGCCACTGGCGTATGCCCAGCAAGAACAGCACCACCTGCTTCAGTCCCAGCCCAAACGCCCCAACTCAGGCACGAATGTAGACCCGAGTACTCATCAGATCCAAAAGGGTCAGTTTTCTGATGCAGGGCAAGAGTTTTTTATGAGAAAACAGCAGCAGCAATTCGGGTTTGCTCAAGGGATGCAGTTTCTGCCTCAGCAGCATCGCATTCAGCAGAAGCCGTTAATGGTGCCGAAACAGAAGGTTGTGCTGGGAAATGGTGAAGAAATGGCTCACCACCAGCAGCAACAACAGCTCCAGCATACTTTGCTCGACCAGCTCTATAAGGCCGCAGAGCTGGTAGGGACTGGGAACTTTTCACACGCGCAAGGGATATTGGCGCGGCTCAATCACCAGCTTTCCCCTGTTGGAAAGCCCCTTCAGAGGGCTGCTTTCTATTTCAAGGAGGCTTTGCAACTCCTCCTCCTCATGAACAATCCTGCCACCTCTCCACCACCGAGAACTCCGACCCCATTTGATATCATCTTCAAAATGGGGGCTTACAAAGTCTTCTCCGAAGTTTCTCCGCTCCTTCAGTTTGTCAATTTCACCTGCAACCAGGCCCTCCTCGAGGCCCTCTCCGACGCTGATCAGATTCACATTGTTGATTTCGATATTGGTTTTGGTGCTCATTGGGCTTCCTTTATGCAGGAGCTTCCCATCAGGAATAGGGGTGCTTCTGCCCCTTCACTCAGAATCACTGCCTTTGCTTCACCTTCGACTCACCACCCGGTTGAGCTCGGGCTTATGTGTGATAATTTGACACAATTCGCTAATGAGATTGGTGTAAGCTTTGATCTTGAAGTGGTTAACATTGATTCTCTGGACCAAAACTCATACTCCCTGCCCATTTTTCGAGCCAACGATAATGAGGCAGTTGCTGTGAATTTCCCCATTTGGTCTACTTCTAATCAACCAGCTGCTTTGCCTAATGTCCTTCGCTTTGTGAAGCAGCTATCTCCCAAAATTGTGGTATCTTTGGATAGAGGGTGTGATAGAAGTGACCTCCCATTCCCACAGCACATTCTGCAGGCCCTCCAGTCGTACATAAACCTTTTGGAATCACTTGATGCTGTTAATGTGACTTCCGATGCTGTGAACAAGATTGAGAGGTTCCTCCTTCAGCCCAAGATCGAGAGCACTGTTCTGGGGCGTCTCCGAACCCCAGACAAGATGCCCCTTTGGAAAACACTCTTTGCCTCCGCTGGTTTCACTCCTGTACCCTTCAGTTCCAGCTTACTCTACTCTGATAATATGTCAATAAATATTGAACTATGTTATTGGCGCCTCTGTTATGACTTAATCCTTATGAATTGTCGTGTTTGTGAGGCCCCTTTGCGTATTGTATATCTAGTCAGATGGACTAGTGATTTCTCGGTAGAACAAATCATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

809

Amino Acids

88.36

Weight (kDa)

5.8

Isoelectric Point (pI)

53.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 435 - 757 5.3e-75 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 1038, 1592
AbsI CCTCGAGG 1 cut(s) 1601
Acc36I ACCTGC 2 cut(s) 1038, 1592
AccB1I GGYRCC 2 cut(s) 1222, 2316
AccBSI CCGCTC 2 cut(s) 528, 1561
AccI GTMKAC 2 cut(s) 1074, 1934
AccII CGCG 2 cut(s) 1358, 1375
AclWI GGATC 3 cut(s) 364, 482, 1087
AcsI RAATTY 3 cut(s) 649, 838, 1918
AcuI CTGAAG 5 cut(s) 1019, 1397, 1550, 2138, 2242
AcyI GRCGYC 4 cut(s) 248, 440, 2185, 2317
AfaI GTAC 4 cut(s) 92, 1084, 2078, 2253
AfiI CCNNNNNNNGG 8 cut(s) 278, 335, 653, 731, 1061, 1416, 1417, 1603
AflIII ACRYGT 1 cut(s) 495
AgsI TTSAA 8 cut(s) 112, 217, 765, 821, 1435, 1522, 1835, 2303
AhdI GACNNNNNGTC 1 cut(s) 496
AhlI ACTAGT 1 cut(s) 2396
AjiI CACGTC 1 cut(s) 498
AjnI CCWGG 1 cut(s) 1590
Alw21I GWGCWC 4 cut(s) 318, 1663, 1773, 2174
Alw26I GTCTC 4 cut(s) 257, 410, 447, 2192
AlwI GGATC 3 cut(s) 364, 482, 1087
AlwNI CAGNNNCTG 5 cut(s) 81, 463, 925, 1183, 1913
Ama87I CYCGRG 4 cut(s) 175, 1078, 1601, 1772
AoxI GGCC 9 cut(s) 306, 345, 604, 643, 1320, 1593, 1605, 2064, 2361
ApaI GGGCCC 2 cut(s) 608, 647
ApoI RAATTY 3 cut(s) 649, 838, 1918
ArsI GACNNNNNNTTYG 2 cut(s) 485, 517
Asp700I GAANNNNTTC 1 cut(s) 989
AspLEI GCGC 3 cut(s) 1360, 1375, 2319
AsuC2I CCSGG 5 cut(s) 447, 583, 726, 905, 1763
AsuHPI GGTGA 6 cut(s) 1259, 1265, 1376, 1573, 1737, 1748
AvaI CYCGRG 4 cut(s) 175, 1078, 1601, 1772
AvaII GGWCC 1 cut(s) 1858
AxyI CCTNAGG 1 cut(s) 966
BaeGI GKGCMC 2 cut(s) 608, 647
BaeI ACNNNNGTAYC 2 cut(s) 2235, 2268
BanI GGYRCC 2 cut(s) 1222, 2316
BanII GRGCYC 7 cut(s) 147, 182, 318, 510, 608, 647, 1773
BbsI GAAGAC 1 cut(s) 1534
Bbv12I GWGCWC 4 cut(s) 318, 1663, 1773, 2174
BbvCI CCTCAGC 2 cut(s) 467, 1188
BccI CCATC 2 cut(s) 1700, 2385
BceAI ACGGC 8 cut(s) 115, 238, 332, 453, 589, 646, 697, 1198
BcgI CGANNNNNNTGC 2 cut(s) 560, 594
BciT130I CCWGG 1 cut(s) 1592
BclI TGATCA 1 cut(s) 1621
BcnI CCSGG 5 cut(s) 447, 583, 726, 905, 1763
BcoDI GTCTC 4 cut(s) 257, 410, 447, 2192
BcuI ACTAGT 1 cut(s) 2396
BfaI CTAG 2 cut(s) 2384, 2397
BfmI CTRYAG 2 cut(s) 82, 2060
BfoI RGCGCY 1 cut(s) 2320
BfuAI ACCTGC 2 cut(s) 1038, 1592
BmcAI AGTACT 1 cut(s) 1084
Bme1390I CCNGG 6 cut(s) 447, 583, 726, 905, 1592, 1763
Bme18I GGWCC 1 cut(s) 1858
BmeRI GACNNNNNGTC 1 cut(s) 496
BmeT110I CYCGRG 4 cut(s) 175, 1078, 1601, 1772
BmgBI CACGTC 1 cut(s) 498
BmiI GGNNCC 6 cut(s) 606, 645, 1224, 2147, 2318, 2364
BmrFI CCNGG 6 cut(s) 447, 583, 726, 905, 1592, 1763
BmrI ACTGGG 1 cut(s) 1350
BmsI GCATC 7 cut(s) 371, 1105, 1164, 1204, 2092, 2113, 2196
BmuI ACTGGG 1 cut(s) 1350
BoxI GACNNNNGTC 1 cut(s) 286
BpiI GAAGAC 1 cut(s) 1534
BplI GAGNNNNNCTC 2 cut(s) 1595, 1627
BpmI CTGGAG 3 cut(s) 302, 1274, 2054
Bpu10I CCTNAGC 2 cut(s) 467, 1188
BpuEI CTTGAG 2 cut(s) 494, 1152
BpuMI CCSGG 5 cut(s) 447, 583, 726, 905, 1763
BsaHI GRCGYC 4 cut(s) 248, 440, 2185, 2317
BsaI GGTCTC 1 cut(s) 410
BsaJI CCNNGG 1 cut(s) 388
BsaWI WCCGGW 1 cut(s) 353
Bsc4I CCNNNNNNNGG 8 cut(s) 278, 335, 653, 731, 1061, 1416, 1417, 1603
Bse118I RCCGGY 2 cut(s) 58, 862
Bse1I ACTGG 5 cut(s) 319, 457, 1005, 1345, 2071
Bse21I CCTNAGG 1 cut(s) 966
Bse3DI GCAATG 2 cut(s) 583, 706
BseBI CCWGG 1 cut(s) 1592
BseDI CCNNGG 1 cut(s) 388
BseGI GGATG 2 cut(s) 490, 1179
BseLI CCNNNNNNNGG 8 cut(s) 278, 335, 653, 731, 1061, 1416, 1417, 1603
BseMI GCAATG 2 cut(s) 583, 706
BseMII CTCAG 7 cut(s) 252, 458, 980, 989, 1074, 1202, 1738
BseNI ACTGG 5 cut(s) 319, 457, 1005, 1345, 2071
BseRI GAGGAG 6 cut(s) 381, 384, 1442, 1445, 1448, 1589
BseSI GKGCMC 2 cut(s) 608, 647
BseYI CCCAGC 6 cut(s) 479, 709, 933, 1011, 1041, 1243
Bsh1236I CGCG 2 cut(s) 1358, 1375
BshFI GGCC 9 cut(s) 308, 347, 606, 645, 1322, 1595, 1607, 2066, 2363
BshNI GGYRCC 2 cut(s) 1222, 2316
BsiHKAI GWGCWC 4 cut(s) 318, 1663, 1773, 2174
BsiHKCI CYCGRG 4 cut(s) 175, 1078, 1601, 1772
BsiSI CCGG 8 cut(s) 59, 354, 446, 582, 725, 863, 905, 1763
BslFI GGGAC 3 cut(s) 294, 1024, 1351
BslI CCNNNNNNNGG 8 cut(s) 278, 335, 653, 731, 1061, 1416, 1417, 1603
BsmAI GTCTC 4 cut(s) 257, 410, 447, 2192
BsmBI CGTCTC 2 cut(s) 447, 2192
BsmFI GGGAC 3 cut(s) 294, 1024, 1351
BsmI GAATGC 1 cut(s) 1200
BsnI GGCC 9 cut(s) 308, 347, 606, 645, 1322, 1595, 1607, 2066, 2363
Bso31I GGTCTC 1 cut(s) 410
BsoBI CYCGRG 4 cut(s) 175, 1078, 1601, 1772
Bsp120I GGGCCC 2 cut(s) 604, 643
Bsp1286I GDGCHC 9 cut(s) 147, 182, 318, 510, 608, 647, 1663, 1773, 2174
Bsp143I GATC 7 cut(s) 356, 474, 598, 1092, 1621, 1828, 2163
BspANI GGCC 9 cut(s) 308, 347, 606, 645, 1322, 1595, 1607, 2066, 2363
BspCNI CTCAG 7 cut(s) 253, 459, 979, 988, 1073, 1201, 1737
BspFNI CGCG 2 cut(s) 1358, 1375
BspHI TCATGA 2 cut(s) 942, 1461
BspLI GGNNCC 6 cut(s) 606, 645, 1224, 2147, 2318, 2364
BspMAI CTGCAG 2 cut(s) 86, 2064
BspMI ACCTGC 2 cut(s) 1038, 1592
BspPI GGATC 3 cut(s) 364, 482, 1087
BspT107I GGYRCC 2 cut(s) 1222, 2316
BspTNI GGTCTC 1 cut(s) 410
BsrBI CCGCTC 2 cut(s) 528, 1561
BsrDI GCAATG 2 cut(s) 583, 706
BsrFI RCCGGY 2 cut(s) 58, 862
BsrI ACTGG 5 cut(s) 319, 457, 1005, 1345, 2071
BssAI RCCGGY 2 cut(s) 58, 862
BssECI CCNNGG 1 cut(s) 388
BssMI GATC 7 cut(s) 356, 474, 598, 1092, 1621, 1828, 2163
BssNI GRCGYC 4 cut(s) 248, 440, 2185, 2317
BssT1I CCWWGG 1 cut(s) 388
Bst2UI CCWGG 1 cut(s) 1592
Bst4CI ACNGT 2 cut(s) 803, 2176
Bst6I CTCTTC 1 cut(s) 211
BstACI GRCGYC 4 cut(s) 248, 440, 2185, 2317
BstAPI GCANNNNNTGC 5 cut(s) 520, 1030, 1183, 1300, 2059
BstC8I GCNNGC 8 cut(s) 60, 143, 255, 273, 310, 314, 876, 2064
BstDEI CTNAG 7 cut(s) 261, 467, 966, 975, 1060, 1188, 1724
BstENI CCTNNNNNAGG 1 cut(s) 1601
BstF5I GGATG 2 cut(s) 490, 1179
BstFNI CGCG 2 cut(s) 1358, 1375
BstH2I RGCGCY 1 cut(s) 2320
BstHHI GCGC 3 cut(s) 1360, 1375, 2319
BstKTI GATC 7 cut(s) 359, 477, 601, 1095, 1624, 1831, 2166
BstMAI GTCTC 4 cut(s) 257, 410, 447, 2192
BstMBI GATC 7 cut(s) 356, 474, 598, 1092, 1621, 1828, 2163
BstNI CCWGG 1 cut(s) 1592
BstPAI GACNNNNGTC 1 cut(s) 286
BstSCI CCNGG 6 cut(s) 445, 581, 724, 903, 1590, 1761
BstSFI CTRYAG 2 cut(s) 82, 2060
BstSLI GKGCMC 2 cut(s) 608, 647
BstUI CGCG 2 cut(s) 1358, 1375
BstV2I GAAGAC 1 cut(s) 1534
BstX2I RGATCY 1 cut(s) 1092
BstXI CCANNNNNNTGG 1 cut(s) 2002
BstYI RGATCY 1 cut(s) 1092
Bsu36I CCTNAGG 1 cut(s) 966
BsuRI GGCC 9 cut(s) 308, 347, 606, 645, 1322, 1595, 1607, 2066, 2363
BtgZI GCGATG 1 cut(s) 1181
BtrI CACGTC 1 cut(s) 498
BtsCI GGATG 2 cut(s) 490, 1179
BtsI GCAGTG 1 cut(s) 1731
BtsIMutI CAGTG 4 cut(s) 808, 998, 1731, 2172
BveI ACCTGC 2 cut(s) 1038, 1592
Cac8I GCNNGC 8 cut(s) 60, 143, 255, 273, 310, 314, 876, 2064
CaiI CAGNNNCTG 5 cut(s) 81, 463, 925, 1183, 1913
CciI TCATGA 2 cut(s) 942, 1461
CfoI GCGC 3 cut(s) 1360, 1375, 2319
Cfr10I RCCGGY 2 cut(s) 58, 862
CseI GACGC 4 cut(s) 256, 429, 1625, 2174
Csp6I GTAC 4 cut(s) 91, 1083, 2077, 2252
CviAII CATG 4 cut(s) 421, 943, 994, 1462
CviQI GTAC 4 cut(s) 91, 1083, 2077, 2252
DdeI CTNAG 7 cut(s) 261, 467, 966, 975, 1060, 1188, 1724
DinI GGCGCC 1 cut(s) 2318
DpnI GATC 7 cut(s) 358, 476, 600, 1094, 1623, 1830, 2165
DpnII GATC 7 cut(s) 356, 474, 598, 1092, 1621, 1828, 2163
DriI GACNNNNNGTC 1 cut(s) 496
Eam1104I CTCTTC 1 cut(s) 211
Eam1105I GACNNNNNGTC 1 cut(s) 496
EarI CTCTTC 1 cut(s) 211
EciI GGCGGA 2 cut(s) 117, 258
Ecl136II GAGCTC 2 cut(s) 316, 1771
Eco130I CCWWGG 1 cut(s) 388
Eco24I GRGCYC 7 cut(s) 147, 182, 318, 510, 608, 647, 1773
Eco31I GGTCTC 1 cut(s) 410
Eco32I GATATC 1 cut(s) 1513
Eco47I GGWCC 1 cut(s) 1858
Eco53kI GAGCTC 2 cut(s) 316, 1771
Eco57I CTGAAG 5 cut(s) 1019, 1397, 1550, 2138, 2242
Eco81I CCTNAGG 1 cut(s) 966
Eco88I CYCGRG 4 cut(s) 175, 1078, 1601, 1772
EcoICRI GAGCTC 2 cut(s) 316, 1771
EcoNI CCTNNNNNAGG 1 cut(s) 1601
EcoO109I RGGNCCY 6 cut(s) 604, 605, 1594, 1606, 2065, 2362
EcoRI GAATTC 1 cut(s) 838
EcoRII CCWGG 1 cut(s) 1590
EcoRV GATATC 1 cut(s) 1513
EcoT14I CCWWGG 1 cut(s) 388
EcoT38I GRGCYC 7 cut(s) 147, 182, 318, 510, 608, 647, 1773
EgeI GGCGCC 1 cut(s) 2318
EheI GGCGCC 1 cut(s) 2318
ErhI CCWWGG 1 cut(s) 388
Esp3I CGTCTC 2 cut(s) 447, 2192
FaeI CATG 4 cut(s) 424, 946, 997, 1465
FalI AAGNNNNNCTT 2 cut(s) 503, 535
FaqI GGGAC 3 cut(s) 294, 1024, 1351
FatI CATG 4 cut(s) 420, 942, 993, 1461
FauI CCCGC 2 cut(s) 392, 521
FauNDI CATATG 1 cut(s) 162
FbaI TGATCA 1 cut(s) 1621
FblI GTMKAC 2 cut(s) 1074, 1934
FokI GGATG 2 cut(s) 497, 1186
FriOI GRGCYC 7 cut(s) 147, 182, 318, 510, 608, 647, 1773
FspBI CTAG 2 cut(s) 2384, 2397
GlaI GCGC 3 cut(s) 1359, 1374, 2318
GsaI CCCAGC 6 cut(s) 483, 713, 937, 1015, 1045, 1247
GsuI CTGGAG 3 cut(s) 302, 1274, 2054
HaeII RGCGCY 1 cut(s) 2320
HaeIII GGCC 9 cut(s) 308, 347, 606, 645, 1322, 1595, 1607, 2066, 2363
HapII CCGG 8 cut(s) 59, 354, 446, 582, 725, 863, 905, 1763
HgaI GACGC 4 cut(s) 256, 429, 1625, 2174
HhaI GCGC 3 cut(s) 1360, 1375, 2319
Hin1I GRCGYC 4 cut(s) 248, 440, 2185, 2317
Hin1II CATG 4 cut(s) 424, 946, 997, 1465
Hin6I GCGC 3 cut(s) 1358, 1373, 2317
HinP1I GCGC 3 cut(s) 1358, 1373, 2317
HincII GTYRAC 1 cut(s) 1843
HindII GTYRAC 1 cut(s) 1843
HindIII AAGCTT 1 cut(s) 1822
HpaI GTTAAC 1 cut(s) 1843
HpaII CCGG 8 cut(s) 59, 354, 446, 582, 725, 863, 905, 1763
HphI GGTGA 6 cut(s) 1259, 1265, 1376, 1573, 1737, 1748
Hpy166II GTNNAC 6 cut(s) 490, 883, 1075, 1843, 1935, 2131
Hpy188III TCNNGA 9 cut(s) 376, 452, 835, 943, 1462, 1696, 1832, 1856, 2167
Hpy8I GTNNAC 6 cut(s) 490, 883, 1075, 1843, 1935, 2131
Hpy99I CGWCG 6 cut(s) 292, 295, 439, 502, 575, 1619
HpyAV CCTTC 9 cut(s) 19, 1228, 1421, 1574, 1728, 1758, 1979, 2162, 2266
HpyCH4III ACNGT 2 cut(s) 803, 2176
HpyCH4IV ACGT 2 cut(s) 287, 497
HpyF3I CTNAG 7 cut(s) 261, 467, 966, 975, 1060, 1188, 1724
HpySE526I ACGT 2 cut(s) 287, 497
Hsp92I GRCGYC 4 cut(s) 248, 440, 2185, 2317
Hsp92II CATG 4 cut(s) 424, 946, 997, 1465
HspAI GCGC 3 cut(s) 1358, 1373, 2317
KasI GGCGCC 1 cut(s) 2316
KroI GCCGGC 1 cut(s) 58
KroNI GCCGGC 1 cut(s) 60
Ksp22I TGATCA 1 cut(s) 1621
KspAI GTTAAC 1 cut(s) 1843
Kzo9I GATC 7 cut(s) 356, 474, 598, 1092, 1621, 1828, 2163
LmnI GCTCC 4 cut(s) 321, 1293, 1566, 1684
LweI GCATC 7 cut(s) 371, 1105, 1164, 1204, 2092, 2113, 2196
MaeI CTAG 2 cut(s) 2384, 2397
MaeII ACGT 2 cut(s) 287, 497
MaeIII GTNAC 5 cut(s) 349, 449, 686, 2033, 2113
MalI GATC 7 cut(s) 358, 476, 600, 1094, 1623, 1830, 2165
MbiI CCGCTC 2 cut(s) 528, 1561
MboI GATC 7 cut(s) 356, 474, 598, 1092, 1621, 1828, 2163
MboII GAAGA 6 cut(s) 149, 180, 198, 1268, 1510, 1534
MfeI CAATTG 1 cut(s) 808
MflI RGATCY 1 cut(s) 1092
MhlI GDGCHC 9 cut(s) 147, 182, 318, 510, 608, 647, 1663, 1773, 2174
Mly113I GGCGCC 1 cut(s) 2317
MlyI GAGTC 3 cut(s) 259, 381, 1747
MmeI TCCRAC 5 cut(s) 158, 384, 1381, 1523, 1638
MroNI GCCGGC 1 cut(s) 58
MroXI GAANNNNTTC 1 cut(s) 989
MseI TTAA 5 cut(s) 899, 1217, 1842, 2109, 2333
MslI CAYNNNNRTG 1 cut(s) 786
MspA1I CMGCKG 6 cut(s) 81, 253, 260, 937, 1952, 2237
MspI CCGG 8 cut(s) 59, 354, 446, 582, 725, 863, 905, 1763
MspR9I CCNGG 6 cut(s) 447, 583, 726, 905, 1592, 1763
MunI CAATTG 1 cut(s) 808
Mva1269I GAATGC 1 cut(s) 1200
MvaI CCWGG 1 cut(s) 1592
MvnI CGCG 2 cut(s) 1358, 1375
NaeI GCCGGC 1 cut(s) 60
NarI GGCGCC 1 cut(s) 2317
NciI CCSGG 5 cut(s) 447, 583, 726, 905, 1763
NdeI CATATG 1 cut(s) 162
NdeII GATC 7 cut(s) 356, 474, 598, 1092, 1621, 1828, 2163
NgoMIV GCCGGC 1 cut(s) 58
NlaIII CATG 4 cut(s) 424, 946, 997, 1465
NlaIV GGNNCC 6 cut(s) 606, 645, 1224, 2147, 2318, 2364
NmuCI GTSAC 4 cut(s) 349, 449, 2033, 2113
PaeR7I CTCGAG 1 cut(s) 1601
PagI TCATGA 2 cut(s) 942, 1461
PaqCI CACCTGC 2 cut(s) 1038, 1592
PcsI WCGNNNNNNNCGW 1 cut(s) 381
PctI GAATGC 1 cut(s) 1200
PdiI GCCGGC 1 cut(s) 60
PdmI GAANNNNTTC 1 cut(s) 989
PfeI GAWTC 8 cut(s) 325, 778, 922, 979, 1627, 1728, 1850, 2093
PflFI GACNNNGTC 1 cut(s) 439
PleI GAGTC 3 cut(s) 259, 380, 1747
PluTI GGCGCC 1 cut(s) 2320
PpsI GAGTC 3 cut(s) 259, 380, 1747
PshAI GACNNNNGTC 1 cut(s) 286
Psp124BI GAGCTC 2 cut(s) 318, 1773
Psp6I CCWGG 1 cut(s) 1590
PspFI CCCAGC 6 cut(s) 479, 709, 933, 1011, 1041, 1243
PspGI CCWGG 1 cut(s) 1590
PspN4I GGNNCC 6 cut(s) 606, 645, 1224, 2147, 2318, 2364
PspOMI GGGCCC 2 cut(s) 604, 643
PspXI VCTCGAGB 1 cut(s) 1601
PstI CTGCAG 2 cut(s) 86, 2064
PstNI CAGNNNCTG 5 cut(s) 81, 463, 925, 1183, 1913
PsuI RGATCY 1 cut(s) 1092
PsyI GACNNNGTC 1 cut(s) 439
PvuII CAGCTG 4 cut(s) 81, 260, 937, 1952
RsaI GTAC 4 cut(s) 92, 1084, 2078, 2253
RsaNI GTAC 4 cut(s) 91, 1083, 2077, 2252
RseI CAYNNNNRTG 1 cut(s) 786
SacI GAGCTC 2 cut(s) 318, 1773
SaqAI TTAA 5 cut(s) 899, 1217, 1842, 2109, 2333
Sau3AI GATC 7 cut(s) 356, 474, 598, 1092, 1621, 1828, 2163
ScaI AGTACT 1 cut(s) 1084
SchI GAGTC 3 cut(s) 259, 381, 1747
ScrFI CCNGG 6 cut(s) 447, 583, 726, 905, 1592, 1763
SduI GDGCHC 9 cut(s) 147, 182, 318, 510, 608, 647, 1663, 1773, 2174
SfaNI GCATC 7 cut(s) 371, 1105, 1164, 1204, 2092, 2113, 2196
SfcI CTRYAG 2 cut(s) 82, 2060
SfoI GGCGCC 1 cut(s) 2318
Sfr274I CTCGAG 1 cut(s) 1601
SinI GGWCC 1 cut(s) 1858
SlaI CTCGAG 1 cut(s) 1601
SmiMI CAYNNNNRTG 1 cut(s) 786
SmlI CTYRAG 3 cut(s) 509, 1167, 1601
SmoI CTYRAG 3 cut(s) 509, 1167, 1601
SpeI ACTAGT 1 cut(s) 2396
SspDI GGCGCC 1 cut(s) 2316
SspI AATATT 1 cut(s) 2299
SspMI CTAG 2 cut(s) 2384, 2397
SstI GAGCTC 2 cut(s) 318, 1773
StyD4I CCNGG 6 cut(s) 445, 581, 724, 903, 1590, 1761
StyI CCWWGG 1 cut(s) 388
TaaI ACNGT 2 cut(s) 803, 2176
TaiI ACGT 2 cut(s) 290, 500
TatI WGTACW 2 cut(s) 90, 1082
TauI GCSGC 8 cut(s) 228, 237, 253, 636, 795, 919, 1325, 1378
TfiI GAWTC 8 cut(s) 325, 778, 922, 979, 1627, 1728, 1850, 2093
Tru1I TTAA 5 cut(s) 899, 1217, 1842, 2109, 2333
Tru9I TTAA 5 cut(s) 899, 1217, 1842, 2109, 2333
TscAI CASTG 4 cut(s) 808, 1005, 1738, 2179
TseFI GTSAC 4 cut(s) 349, 449, 2033, 2113
Tsp45I GTSAC 4 cut(s) 349, 449, 2033, 2113
TspDTI ATGAA 5 cut(s) 149, 931, 982, 1478, 2357
TspRI CASTG 4 cut(s) 808, 1005, 1738, 2179
Tth111I GACNNNGTC 1 cut(s) 439
VpaK11BI GGWCC 1 cut(s) 1858
XagI CCTNNNNNAGG 1 cut(s) 1601
XapI RAATTY 3 cut(s) 649, 838, 1918
XhoI CTCGAG 1 cut(s) 1601
XmiI GTMKAC 2 cut(s) 1074, 1934
XmnI GAANNNNTTC 1 cut(s) 989
XspI CTAG 2 cut(s) 2384, 2397
ZrmI AGTACT 1 cut(s) 1084
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.