MD04G1088100.v1.1

Scarecrow-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0002481
Physical Location & Seq
Reverse (-)
13542153 .. 13544592
2440 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1088100.v1.1.491

Sequence Viewer

Length: 2385 bp
ATGCCCTTTCAGTTCCAGCAGGGGAAGGGGGTTGTCGAAATCGCAACCGCAAGTTTACCGGCAATTTTCACAGACAGCAGCTGCAGCAAGTACAAAGACGGCGGCTTTTTCAACCACCAACACCACAGCTTTCCGACCGGCGAGCCCAACTCTGTTCTTCATATGAGAAGAAGCCCGAGCCCACCCACTTCGGCTTCCACTCTCTCTTCGTCTTTTAACAACGGCGGTGGTGGCGGTGACAAATCGACGTCGCTGGCTGCGGAGACTCTGCCTGCTGGGGGTCCAGCGTCGTCCAAAGACGAGTGGGCCTGCGAGCTCCAGTCGATTCGGAGCGGCGGATTGGAGGCCGTGACCGGATCGGGAGGAGGGGTCGAGAAATGCGGCCTTGGGCTTGGCGGGTTGGAGGATTGGGAGACTATGCTCTCCGAAACGGCAGCGTCTCCGGCCCACGACCAGTCTCTGCTGAGGTGGATAGCCGGGGATGTGGACGACATGTCGTGTGGACTCAAGCAGCTGTTGCAGAGCGGGAACGGCAACCACCACAACAGCCCCATTGATTTCGACAGCAATGCCGGGTTGGGAATCGTTGATCAGGGCCCTAATTTCGATATAATTGGGAGCAGCGGCGTTTCGGTCCCGAATTTCAGGTTCGCCGGTTCGGGGTTTTCAAACGGCGGCAACAATGGCAGCAATGGGAAGGTGGGTTATGTTCCTCCCGGGTCTGGGTCTTTTGGTTTGCTAAATTACAAGGTTTCAAAGGTTGGGTTAATGCACAACAATGGCGGCACCAACAGTGTCAATTGCAACATTCAAAACCCCATTTTCTCGAATTCGTCCAACACTGTGGCTCTGCCGGTCTCTTTGCCTGCGGTTTACGGGCAGCAGCAATTTGAGAGCAGCCCGGACGAGAAGCCGCAGATTCTGAACCCGCAGCTTTTCATGAGCAACCAGACTCAGCAGCCACAAAACCCGAACTTTTTCATGCCACTGGCGTATGCCCAGCAAGAACAGTACCACCTGCTTCAGTCCCAGCCCAAACGCCACAACTCCGGCTCGAATGTAGACCTGAGTACTCATCAGATCCAAAAGGGTCAGTTTTCTGATCCGAGACACGAGATTTTTGTGAGGCACCAGCAGCAGCAGCAATTGGGGTTTGCTCAAGGGATGCGGTTTCTACCTCAGCAGCATCACATTCAGCAGAAGCCGTTGATGGTGCCGAAACAGACGGTTGTGCTGGGAAATGGTGAAGAAATGACCCACCAGCAGCAGCAACAAGAGCTCCAGGATACTTTGCTCGACCAGCTCTATAAGGCCGCAGAACTGGTAGGGACTGGGAACTTTTCACACGCGCAAGGGATATTGGCGCGGCTCAATCACCAGCTTTCCCCTGTTGGAAAGCCCCTTCAGAGGGCTGCTTTCTATTTCAAGGAGGCTTTGCAACTCCTCATGAACAATCCTGCCACCTTTCCGCCACCGAGAACTCTGACCCCATTTGATGTCTTCTTCAAAATGGGGGCATACAAAGTGTTCTCTGAAGTTTCTCCGCTCCTTCAGTTTGTCAATTTCACCTGCAACCAGGCCCTCCTTGAGGCCCTCTCAGACGCTGATCAGATTCACATTGTTGATTTCGATATTGGGTTTGGCGCTCATTGGGCTTCATTTATGCAGGAGCTTCCTACCCGGAATAGGGGTATTTCTGCCCCTTCACTCAGAATCACTGCCTTTGCCTCTCCTTCAACTCACCACCCAGTTGAGCTCGGGCTTATGCGTGATAATTTGACACAATTTGCTAATGAGATTGGTATAAGTTTTGAGCTTGACGTGGTTAACCTTGATTATCTGGACCAAAACTCCTACTCCCTGCCCATTTTTCAAACCAACGATAGTGAGGCAGTTGCTGTGAATTTCCCCATTTGGTCTACATCGAATCAACCGGATGCTTTACCAAATGTCCTTCGCTTTGTGAAGCAGCTATCTCCCAAAATTGTGGTGTCCTTGGATAGAGGTTGTGATAGAAGTGACCTCCCGTTCCCGCAGCACATTCTGCAGGCCCTCCAGTCCTACATAAACCTTTTGGAGTCACTTGATGCGGTTAATGTGACTTCCGATGCTGTGAGCAAGATTGAGAGGTTCCTCCTTCAGCCCAAGATTGAGAGCACTGTTCTGGGGCGTCTCCGAACCCCAGACAAGATGCCCATTTGGAAAGCACTCTTTGCCTCTGCCGGTTTCACTCCTGTACCCTTCAGTAACTTCACTGAAACTCAGGCGGAATGTGTGGTGAAGAGGAATCCAGCAAGGGGATTCCATGTTGAGAAGCGTCAAGAGTCACTTGTTCTATGCTGGCAGCGACGAGAGCTCATCTCTGCTTCAGCTTGGAGGTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

795

Amino Acids

86.88

Weight (kDa)

6.0

Isoelectric Point (pI)

55.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 432 - 793 5.3e-93 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 1026, 1577
AatII GACGTC 1 cut(s) 251
Acc36I ACCTGC 2 cut(s) 1026, 1577
AccB1I GGYRCC 3 cut(s) 785, 1128, 1213
AccBSI CCGCTC 3 cut(s) 333, 525, 1546
AccI GTMKAC 2 cut(s) 1062, 1919
AccII CGCG 2 cut(s) 1349, 1366
AclWI GGATC 3 cut(s) 364, 1075, 1097
AcsI RAATTY 3 cut(s) 640, 829, 1903
AcuI CTGAAG 7 cut(s) 1007, 1388, 1535, 1554, 2123, 2227, 2352
AcyI GRCGYC 2 cut(s) 248, 2170
AfaI GTAC 4 cut(s) 92, 1013, 1072, 2238
AflIII ACRYGT 1 cut(s) 492
AgsI TTSAA 8 cut(s) 112, 669, 756, 812, 1426, 1507, 1737, 1874
AhdI GACNNNNNGTC 1 cut(s) 493
AjiI CACGTC 1 cut(s) 1822
AjnI CCWGG 2 cut(s) 1281, 1575
Alw21I GWGCWC 5 cut(s) 318, 1281, 1758, 2159, 2358
Alw26I GTCTC 7 cut(s) 257, 407, 444, 462, 862, 1102, 2177
AlwI GGATC 3 cut(s) 364, 1075, 1097
AlwNI CAGNNNCTG 5 cut(s) 81, 460, 922, 1604, 1898
Ama87I CYCGRG 3 cut(s) 175, 716, 1757
AoxI GGCC 9 cut(s) 306, 345, 382, 444, 595, 1311, 1578, 1590, 2049
ApaI GGGCCC 1 cut(s) 599
ApoI RAATTY 3 cut(s) 640, 829, 1903
Asp700I GAANNNNTTC 1 cut(s) 977
AspLEI GCGC 3 cut(s) 1351, 1366, 1646
AsuC2I CCSGG 6 cut(s) 478, 574, 717, 718, 902, 1681
AsuHPI GGTGA 6 cut(s) 248, 1256, 1367, 1558, 1733, 2290
AvaI CYCGRG 3 cut(s) 175, 716, 1757
AvaII GGWCC 3 cut(s) 281, 634, 1843
BaeGI GKGCMC 1 cut(s) 599
BaeI ACNNNNGTAYC 2 cut(s) 2220, 2253
BanI GGYRCC 3 cut(s) 785, 1128, 1213
BanII GRGCYC 7 cut(s) 147, 182, 318, 599, 1281, 1758, 2358
BauI CACGAG 1 cut(s) 1112
BbsI GAAGAC 1 cut(s) 1492
Bbv12I GWGCWC 5 cut(s) 318, 1281, 1758, 2159, 2358
BbvCI CCTCAGC 2 cut(s) 464, 1179
BccI CCATC 1 cut(s) 1204
BceAI ACGGC 7 cut(s) 115, 238, 332, 447, 547, 688, 1189
BcgI CGANNNNNNTGC 2 cut(s) 551, 585
BciT130I CCWGG 2 cut(s) 1283, 1577
BciVI GTATCC 1 cut(s) 1279
BclI TGATCA 2 cut(s) 589, 1606
BcnI CCSGG 6 cut(s) 478, 574, 717, 718, 902, 1681
BcoDI GTCTC 7 cut(s) 257, 407, 444, 462, 862, 1102, 2177
BfmI CTRYAG 2 cut(s) 82, 2045
BfoI RGCGCY 1 cut(s) 1647
BfuAI ACCTGC 2 cut(s) 1026, 1577
BfuI GTATCC 1 cut(s) 1279
BmcAI AGTACT 1 cut(s) 1072
Bme1390I CCNGG 8 cut(s) 478, 574, 717, 718, 902, 1283, 1577, 1681
Bme18I GGWCC 3 cut(s) 281, 634, 1843
BmeRI GACNNNNNGTC 1 cut(s) 493
BmeT110I CYCGRG 3 cut(s) 175, 716, 1757
BmgBI CACGTC 1 cut(s) 1822
BmiI GGNNCC 7 cut(s) 282, 597, 636, 787, 1130, 1215, 2132
BmrFI CCNGG 8 cut(s) 478, 574, 717, 718, 902, 1283, 1577, 1681
BmrI ACTGGG 2 cut(s) 1341, 1742
BmsI GCATC 6 cut(s) 1155, 1195, 1927, 2077, 2098, 2181
BmuI ACTGGG 2 cut(s) 1341, 1742
BpiI GAAGAC 1 cut(s) 1492
BplI GAGNNNNNCTC 6 cut(s) 134, 166, 1580, 1612, 2345, 2377
BpmI CTGGAG 3 cut(s) 302, 1265, 2039
Bpu10I CCTNAGC 2 cut(s) 464, 1179
BpuEI CTTGAG 3 cut(s) 491, 1143, 1607
BpuMI CCSGG 6 cut(s) 478, 574, 717, 718, 902, 1681
BsaHI GRCGYC 2 cut(s) 248, 2170
BsaI GGTCTC 1 cut(s) 862
BsaJI CCNNGG 4 cut(s) 385, 477, 716, 1995
BsaWI WCCGGW 2 cut(s) 353, 1933
BsaXI ACNNNNNCTCC 8 cut(s) 354, 384, 1263, 1293, 1835, 1841, 1865, 1871
Bse118I RCCGGY 5 cut(s) 58, 137, 653, 853, 2222
Bse1I ACTGG 7 cut(s) 319, 454, 993, 1326, 1336, 1748, 2056
Bse3DI GCAATG 2 cut(s) 574, 697
BseBI CCWGG 2 cut(s) 1283, 1577
BseDI CCNNGG 4 cut(s) 385, 477, 716, 1995
BseGI GGATG 3 cut(s) 487, 1170, 1942
BseMI GCAATG 2 cut(s) 574, 697
BseMII CTCAG 7 cut(s) 455, 968, 1058, 1193, 1611, 1723, 2276
BseNI ACTGG 7 cut(s) 319, 454, 993, 1326, 1336, 1748, 2056
BseRI GAGGAG 2 cut(s) 378, 1433
BseSI GKGCMC 1 cut(s) 599
BseYI CCCAGC 4 cut(s) 275, 999, 1029, 1234
Bsh1236I CGCG 2 cut(s) 1349, 1366
Bsh1285I CGRYCG 1 cut(s) 138
BshFI GGCC 9 cut(s) 308, 347, 384, 446, 597, 1313, 1580, 1592, 2051
BshNI GGYRCC 3 cut(s) 785, 1128, 1213
BsiEI CGRYCG 1 cut(s) 138
BsiHKAI GWGCWC 5 cut(s) 318, 1281, 1758, 2159, 2358
BsiHKCI CYCGRG 3 cut(s) 175, 716, 1757
BslFI GGGAC 3 cut(s) 620, 1012, 1342
BsmAI GTCTC 7 cut(s) 257, 407, 444, 462, 862, 1102, 2177
BsmBI CGTCTC 2 cut(s) 444, 2177
BsmFI GGGAC 3 cut(s) 620, 1012, 1342
BsnI GGCC 9 cut(s) 308, 347, 384, 446, 597, 1313, 1580, 1592, 2051
Bso31I GGTCTC 1 cut(s) 862
BsoBI CYCGRG 3 cut(s) 175, 716, 1757
Bsp120I GGGCCC 1 cut(s) 595
Bsp1286I GDGCHC 8 cut(s) 147, 182, 318, 599, 1281, 1758, 2159, 2358
Bsp143I GATC 5 cut(s) 356, 589, 1080, 1102, 1606
BspANI GGCC 9 cut(s) 308, 347, 384, 446, 597, 1313, 1580, 1592, 2051
BspCNI CTCAG 7 cut(s) 456, 967, 1059, 1192, 1610, 1722, 2275
BspFNI CGCG 2 cut(s) 1349, 1366
BspHI TCATGA 2 cut(s) 939, 1446
BspLI GGNNCC 7 cut(s) 282, 597, 636, 787, 1130, 1215, 2132
BspMAI CTGCAG 2 cut(s) 86, 2049
BspMI ACCTGC 2 cut(s) 1026, 1577
BspPI GGATC 3 cut(s) 364, 1075, 1097
BspT107I GGYRCC 3 cut(s) 785, 1128, 1213
BspTNI GGTCTC 1 cut(s) 862
BsrBI CCGCTC 3 cut(s) 333, 525, 1546
BsrDI GCAATG 2 cut(s) 574, 697
BsrFI RCCGGY 5 cut(s) 58, 137, 653, 853, 2222
BsrI ACTGG 7 cut(s) 319, 454, 993, 1326, 1336, 1748, 2056
BssAI RCCGGY 5 cut(s) 58, 137, 653, 853, 2222
BssECI CCNNGG 4 cut(s) 385, 477, 716, 1995
BssMI GATC 5 cut(s) 356, 589, 1080, 1102, 1606
BssNI GRCGYC 2 cut(s) 248, 2170
BssSI CACGAG 1 cut(s) 1112
BssT1I CCWWGG 2 cut(s) 385, 1995
Bst2BI CACGAG 1 cut(s) 1112
Bst2UI CCWGG 2 cut(s) 1283, 1577
Bst4CI ACNGT 5 cut(s) 794, 844, 1011, 1228, 2161
Bst6I CTCTTC 2 cut(s) 211, 2276
BstACI GRCGYC 2 cut(s) 248, 2170
BstAPI GCANNNNNTGC 3 cut(s) 517, 2044, 2213
BstC8I GCNNGC 8 cut(s) 143, 255, 273, 310, 314, 867, 2049, 2342
BstDEI CTNAG 7 cut(s) 464, 954, 1067, 1179, 1597, 1709, 2262
BstENI CCTNNNNNAGG 1 cut(s) 1586
BstF5I GGATG 3 cut(s) 487, 1170, 1942
BstFNI CGCG 2 cut(s) 1349, 1366
BstH2I RGCGCY 1 cut(s) 1647
BstHHI GCGC 3 cut(s) 1351, 1366, 1646
BstKTI GATC 5 cut(s) 359, 592, 1083, 1105, 1609
BstMAI GTCTC 7 cut(s) 257, 407, 444, 462, 862, 1102, 2177
BstMBI GATC 5 cut(s) 356, 589, 1080, 1102, 1606
BstMCI CGRYCG 1 cut(s) 138
BstNI CCWGG 2 cut(s) 1283, 1577
BstNSI RCATGY 1 cut(s) 496
BstSCI CCNGG 8 cut(s) 476, 572, 715, 716, 900, 1281, 1575, 1679
BstSFI CTRYAG 2 cut(s) 82, 2045
BstSLI GKGCMC 1 cut(s) 599
BstUI CGCG 2 cut(s) 1349, 1366
BstV2I GAAGAC 1 cut(s) 1492
BstX2I RGATCY 1 cut(s) 1080
BstXI CCANNNNNNTGG 2 cut(s) 844, 1987
BstYI RGATCY 1 cut(s) 1080
BsuI GTATCC 1 cut(s) 1279
BsuRI GGCC 9 cut(s) 308, 347, 384, 446, 597, 1313, 1580, 1592, 2051
BtrI CACGTC 1 cut(s) 1822
BtsCI GGATG 3 cut(s) 487, 1170, 1942
BtsI GCAGTG 1 cut(s) 1716
BtsIMutI CAGTG 6 cut(s) 799, 840, 986, 1716, 2157, 2253
BveI ACCTGC 2 cut(s) 1026, 1577
Cac8I GCNNGC 8 cut(s) 143, 255, 273, 310, 314, 867, 2049, 2342
CaiI CAGNNNCTG 5 cut(s) 81, 460, 922, 1604, 1898
CciI TCATGA 2 cut(s) 939, 1446
CfoI GCGC 3 cut(s) 1351, 1366, 1646
Cfr10I RCCGGY 5 cut(s) 58, 137, 653, 853, 2222
Cfr9I CCCGGG 1 cut(s) 716
CseI GACGC 5 cut(s) 276, 426, 1610, 2159, 2306
Csp6I GTAC 4 cut(s) 91, 1012, 1071, 2237
CspCI CAANNNNNGTGG 2 cut(s) 208, 243
CviAII CATG 5 cut(s) 493, 940, 982, 1447, 2306
CviQI GTAC 4 cut(s) 91, 1012, 1071, 2237
DdeI CTNAG 7 cut(s) 464, 954, 1067, 1179, 1597, 1709, 2262
DpnI GATC 5 cut(s) 358, 591, 1082, 1104, 1608
DpnII GATC 5 cut(s) 356, 589, 1080, 1102, 1606
DriI GACNNNNNGTC 1 cut(s) 493
Eam1104I CTCTTC 2 cut(s) 211, 2276
Eam1105I GACNNNNNGTC 1 cut(s) 493
EarI CTCTTC 2 cut(s) 211, 2276
EciI GGCGGA 3 cut(s) 351, 1458, 2282
Ecl136II GAGCTC 4 cut(s) 316, 1279, 1756, 2356
Eco130I CCWWGG 2 cut(s) 385, 1995
Eco24I GRGCYC 7 cut(s) 147, 182, 318, 599, 1281, 1758, 2358
Eco31I GGTCTC 1 cut(s) 862
Eco47I GGWCC 3 cut(s) 281, 634, 1843
Eco53kI GAGCTC 4 cut(s) 316, 1279, 1756, 2356
Eco57I CTGAAG 7 cut(s) 1007, 1388, 1535, 1554, 2123, 2227, 2352
Eco88I CYCGRG 3 cut(s) 175, 716, 1757
EcoICRI GAGCTC 4 cut(s) 316, 1279, 1756, 2356
EcoNI CCTNNNNNAGG 1 cut(s) 1586
EcoO109I RGGNCCY 5 cut(s) 595, 596, 1579, 1591, 2050
EcoRI GAATTC 1 cut(s) 829
EcoRII CCWGG 2 cut(s) 1281, 1575
EcoT14I CCWWGG 2 cut(s) 385, 1995
EcoT38I GRGCYC 7 cut(s) 147, 182, 318, 599, 1281, 1758, 2358
ErhI CCWWGG 2 cut(s) 385, 1995
Esp3I CGTCTC 2 cut(s) 444, 2177
FaeI CATG 5 cut(s) 496, 943, 985, 1450, 2309
FalI AAGNNNNNCTT 2 cut(s) 2313, 2345
FaqI GGGAC 3 cut(s) 620, 1012, 1342
FatI CATG 5 cut(s) 492, 939, 981, 1446, 2305
FauI CCCGC 4 cut(s) 389, 518, 936, 2040
FauNDI CATATG 1 cut(s) 162
FbaI TGATCA 2 cut(s) 589, 1606
FblI GTMKAC 2 cut(s) 1062, 1919
FokI GGATG 3 cut(s) 494, 1177, 1949
FriOI GRGCYC 7 cut(s) 147, 182, 318, 599, 1281, 1758, 2358
GlaI GCGC 3 cut(s) 1350, 1365, 1645
GsaI CCCAGC 4 cut(s) 279, 1003, 1033, 1238
GsuI CTGGAG 3 cut(s) 302, 1265, 2039
HaeII RGCGCY 1 cut(s) 1647
HaeIII GGCC 9 cut(s) 308, 347, 384, 446, 597, 1313, 1580, 1592, 2051
HgaI GACGC 5 cut(s) 276, 426, 1610, 2159, 2306
HhaI GCGC 3 cut(s) 1351, 1366, 1646
Hin1I GRCGYC 2 cut(s) 248, 2170
Hin1II CATG 5 cut(s) 496, 943, 985, 1450, 2309
Hin6I GCGC 3 cut(s) 1349, 1364, 1644
HinP1I GCGC 3 cut(s) 1349, 1364, 1644
HincII GTYRAC 1 cut(s) 1828
HindII GTYRAC 1 cut(s) 1828
HpaI GTTAAC 1 cut(s) 1828
HphI GGTGA 6 cut(s) 248, 1256, 1367, 1558, 1733, 2290
Hpy166II GTNNAC 7 cut(s) 56, 487, 503, 874, 1063, 1828, 1920
Hpy188III TCNNGA 8 cut(s) 360, 373, 637, 826, 940, 1447, 1841, 2321
Hpy8I GTNNAC 7 cut(s) 56, 487, 503, 874, 1063, 1828, 1920
Hpy99I CGWCG 4 cut(s) 250, 253, 292, 2352
HpyAV CCTTC 9 cut(s) 19, 691, 1412, 1559, 1713, 1743, 1964, 2147, 2251
HpyCH4III ACNGT 5 cut(s) 794, 844, 1011, 1228, 2161
HpyCH4IV ACGT 2 cut(s) 248, 1821
HpyCH4V TGCA 8 cut(s) 84, 520, 772, 804, 1438, 1572, 1666, 2047
HpyF3I CTNAG 7 cut(s) 464, 954, 1067, 1179, 1597, 1709, 2262
HpySE526I ACGT 2 cut(s) 248, 1821
Hsp92I GRCGYC 2 cut(s) 248, 2170
Hsp92II CATG 5 cut(s) 496, 943, 985, 1450, 2309
HspAI GCGC 3 cut(s) 1349, 1364, 1644
Ksp22I TGATCA 2 cut(s) 589, 1606
KspAI GTTAAC 1 cut(s) 1828
Kzo9I GATC 5 cut(s) 356, 589, 1080, 1102, 1606
LmnI GCTCC 6 cut(s) 321, 330, 618, 1284, 1551, 1669
LweI GCATC 6 cut(s) 1155, 1195, 1927, 2077, 2098, 2181
MaeII ACGT 2 cut(s) 248, 1821
MaeIII GTNAC 7 cut(s) 236, 349, 2018, 2079, 2098, 2246, 2325
MalI GATC 5 cut(s) 358, 591, 1082, 1104, 1608
MbiI CCGCTC 3 cut(s) 333, 525, 1546
MboI GATC 5 cut(s) 356, 589, 1080, 1102, 1606
MboII GAAGA 7 cut(s) 149, 180, 198, 1259, 1492, 1495, 2293
MfeI CAATTG 2 cut(s) 799, 1145
MflI RGATCY 1 cut(s) 1080
MhlI GDGCHC 8 cut(s) 147, 182, 318, 599, 1281, 1758, 2159, 2358
MlyI GAGTC 5 cut(s) 259, 498, 946, 2087, 2333
MmeI TCCRAC 4 cut(s) 158, 381, 861, 1372
MroXI GAANNNNTTC 1 cut(s) 977
MseI TTAA 4 cut(s) 216, 767, 1827, 2094
MslI CAYNNNNRTG 1 cut(s) 777
MspA1I CMGCKG 3 cut(s) 81, 514, 624
MspR9I CCNGG 8 cut(s) 478, 574, 717, 718, 902, 1283, 1577, 1681
MunI CAATTG 2 cut(s) 799, 1145
MvaI CCWGG 2 cut(s) 1283, 1577
MvnI CGCG 2 cut(s) 1349, 1366
NciI CCSGG 6 cut(s) 478, 574, 717, 718, 902, 1681
NdeI CATATG 1 cut(s) 162
NdeII GATC 5 cut(s) 356, 589, 1080, 1102, 1606
NlaIII CATG 5 cut(s) 496, 943, 985, 1450, 2309
NlaIV GGNNCC 7 cut(s) 282, 597, 636, 787, 1130, 1215, 2132
NmuCI GTSAC 6 cut(s) 236, 349, 2018, 2079, 2098, 2325
NspI RCATGY 1 cut(s) 496
PagI TCATGA 2 cut(s) 939, 1446
PaqCI CACCTGC 2 cut(s) 1026, 1577
PciI ACATGT 1 cut(s) 492
PdmI GAANNNNTTC 1 cut(s) 977
PfeI GAWTC 8 cut(s) 325, 582, 919, 1612, 1713, 1927, 2287, 2301
PfoI TCCNGGA 1 cut(s) 1281
PleI GAGTC 5 cut(s) 259, 498, 946, 2086, 2332
PpsI GAGTC 5 cut(s) 259, 498, 946, 2086, 2332
PscI ACATGT 1 cut(s) 492
Psp124BI GAGCTC 4 cut(s) 318, 1281, 1758, 2358
Psp6I CCWGG 2 cut(s) 1281, 1575
PspFI CCCAGC 4 cut(s) 275, 999, 1029, 1234
PspGI CCWGG 2 cut(s) 1281, 1575
PspN4I GGNNCC 7 cut(s) 282, 597, 636, 787, 1130, 1215, 2132
PspOMI GGGCCC 1 cut(s) 595
PstI CTGCAG 2 cut(s) 86, 2049
PstNI CAGNNNCTG 5 cut(s) 81, 460, 922, 1604, 1898
PsuI RGATCY 1 cut(s) 1080
PvuII CAGCTG 2 cut(s) 81, 514
RsaI GTAC 4 cut(s) 92, 1013, 1072, 2238
RsaNI GTAC 4 cut(s) 91, 1012, 1071, 2237
RseI CAYNNNNRTG 1 cut(s) 777
SacI GAGCTC 4 cut(s) 318, 1281, 1758, 2358
SaqAI TTAA 4 cut(s) 216, 767, 1827, 2094
Sau3AI GATC 5 cut(s) 356, 589, 1080, 1102, 1606
ScaI AGTACT 1 cut(s) 1072
SchI GAGTC 5 cut(s) 259, 498, 946, 2087, 2333
ScrFI CCNGG 8 cut(s) 478, 574, 717, 718, 902, 1283, 1577, 1681
SduI GDGCHC 8 cut(s) 147, 182, 318, 599, 1281, 1758, 2159, 2358
SfaNI GCATC 6 cut(s) 1155, 1195, 1927, 2077, 2098, 2181
SfcI CTRYAG 2 cut(s) 82, 2045
SinI GGWCC 3 cut(s) 281, 634, 1843
SmaI CCCGGG 1 cut(s) 718
SmiMI CAYNNNNRTG 1 cut(s) 777
SmlI CTYRAG 3 cut(s) 506, 1158, 1586
SmoI CTYRAG 3 cut(s) 506, 1158, 1586
SstI GAGCTC 4 cut(s) 318, 1281, 1758, 2358
StyD4I CCNGG 8 cut(s) 476, 572, 715, 716, 900, 1281, 1575, 1679
StyI CCWWGG 2 cut(s) 385, 1995
TaaI ACNGT 5 cut(s) 794, 844, 1011, 1228, 2161
TaiI ACGT 2 cut(s) 251, 1824
TaqII GACCGA 1 cut(s) 622
TatI WGTACW 2 cut(s) 90, 1070
TauI GCSGC 9 cut(s) 105, 336, 384, 627, 678, 786, 916, 1316, 1369
TfiI GAWTC 8 cut(s) 325, 582, 919, 1612, 1713, 1927, 2287, 2301
Tru1I TTAA 4 cut(s) 216, 767, 1827, 2094
Tru9I TTAA 4 cut(s) 216, 767, 1827, 2094
TscAI CASTG 6 cut(s) 799, 847, 993, 1723, 2164, 2260
TseFI GTSAC 6 cut(s) 236, 349, 2018, 2079, 2098, 2325
Tsp45I GTSAC 6 cut(s) 236, 349, 2018, 2079, 2098, 2325
TspDTI ATGAA 5 cut(s) 149, 928, 970, 1463, 1647
TspMI CCCGGG 1 cut(s) 716
TspRI CASTG 6 cut(s) 799, 847, 993, 1723, 2164, 2260
VpaK11BI GGWCC 3 cut(s) 281, 634, 1843
XagI CCTNNNNNAGG 1 cut(s) 1586
XapI RAATTY 3 cut(s) 640, 829, 1903
XceI RCATGY 1 cut(s) 496
XmaI CCCGGG 1 cut(s) 716
XmiI GTMKAC 2 cut(s) 1062, 1919
XmnI GAANNNNTTC 1 cut(s) 977
ZraI GACGTC 1 cut(s) 249
ZrmI AGTACT 1 cut(s) 1072
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.