Rh7BG202000

Scarecrow-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0002481
Physical Location & Seq
Forward (+)
16800018 .. 16803833
3816 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG202000.1

Sequence Viewer

Length: 2355 bp
ATGCCCTTTCAATTTCAGCAACAGGGGAAGGGGGTCTTAGAAATTACAGCGGCATCAATCTGTGACTGGAACCACAAGAAAGACGAGGAGCTTTTCATCAACAACAGCTTCGTCAGCAACGAACCTACCTCTGTTCTTCATATGAGAAGAAGCCCGAGCCCACCCACTTCGGCATCCACTCTCTCCTCCTCCTTCAACGGCGGCGGCAACGGTAACACCACCACGCCGCTTCTGCAGGACACTTCGCCGGTGGGACCCGAGCCCGACACGGCGGCGGCGCGCAGCAGCAGCAAGGATGAGTGGACCTGCGAGCTTCAGTCGATTCCCAGCGGCGGTCTGGAGGCCGTGACCGGATCGGGCGGCGGGAGATGCGGCGGGCTTGGGTTGGAGGACTGGGAGACCATGCTGTCCGAAACGGCGGCGTCTCCGGGTCAAGACCAGTCGCTGCTCCGGTGGATCTCGGGCGACGTGGACGACGCGTCGTTTGGGCTAAAGCAGCTGTTGCAGAGCGGGAACAACAGTAACTCTCTTGAGTTTGAAGGCAATGCGGGTCTGGGAATGGTCGATCAGGGTTCCGGTTTCGACCACATCGGGAGCAGCGTTTCCGGTCCCAATTTGGGGTTCTCCGGCGGTTCTGGTTTCAATGGGAAAATGGGTTTTGTGCCCAGCTCTTCTGGGCCGCTGAATTACAAGGTTTCAAATGTCGGGTTGATGAACAATAATGGCGGCAACAACAACAACAACAACTCCAATTTCGCCAATTCAGCACTTCCAGTTTCGCTGCCTCATGGAATGGTTTATCAGAATCACCAGCAGTTTGAGAACCCAGATGAGAAGCCTCAGATTCTGAACCCACAGCTGTTCATGAACCAGCACCAGTCTCAGCTCCCTCATCAAAATCCAAACTTTTTCATGCCAGTGACTTATGCTCAGCAGGAGCAGCACTTTCTTCAGTCCCAGCCCAAGCGCCACAACCCAGGTGGAGGCGGTGGAGGACTGGACCCGAGTTCTCAGATCCACCAAAAGGTACAATATTCTGATCCGGGTCATGAGTTTCTGCTCAGAAAGCAACACCTTCAGCAGCAGCAGCAGCAGCCCCATTTTGGTAATTTCCCTCCCGGAATGCAGTTCCTTCCTCAGCAGCACCACACTCAACCGAGGCCGGTAGTGGTGCCCAAGCAGAAGGTGGGTGGCGAAGAAATGGCGGCGGCGGCGGCTAACCAGAACCAGCAGCAGCAACTGCTACAGCATAGTTTGCTGGACCAGCTCTATAAGGCCGCAGAAATGGTAGGTACTGGGAACTTCTCACACGCGCAGGCGATATTGGCGCGGCTCAATCACCAGCTCTCCCCTGTTGGAAAGCCCCTTCAGCGGGCTGCTTTCTATTTCAAGGAGGCTTTGCAACTCCTCCTCCTTATGAACAACCCAGCAACCTCTCCTCCACCAAGAACCCCGACCCCTTTTGATGTCATCTTCAAAATGGGGGCTTACAAAGTCTTCTCTGAAGTGTCTCCATTGATTCAGTTTGTCAATTTTACTTGCAACCAGGCTCTACTTGAGGCTGTATCAGAAGCTGATCAGATTCACATTGTTGATTTTGATATTGGGTTTGGTGCTCACTGGGCCTCCTTTATGCAGGAACTCCCATTGAGGAACAGGGGTGGCACTTCCCCCTTTCTCAAAATCACTGCTTTTGCCTCTCCTTCTACTCATCACACAGTTGAGCTTAATCTTATGCGTGATAATTTAACCCAATTTGCTGATGAGATTGGCTTAGGTTTTGAGCTTGAAGTAGTTAACATTGATTTGTTGGACCAAAACTCATACTCACTGCCCTTTTTCCGAGCTAATGATAATGAGGCAGTTGCTGTGAATTTCCCCATTTGGTCTAGTTCGAATCAACCGGCTGCTTTGCCCAATCTCCTTCGATTTGTTAAACAGCTCTCTCCCAAAATTGTGGTGTCTTTGGATAGAGGGTGTGACAGGAGTGACCTTCCATTCCCACAGCACATTCTGCAGGCCCTCCAATCGTACATAAACCTCTTGGAATCTCTTGATGCGGTTAATGTAACTTCGGATGCTGTGAACAAGATTGAGAGGTTCCTCCTTCAGCCTAAGATTGAAAGCACTGTTTTGGGGCGTCTCCGAACCCCTGACAAAATGCCCCTATGGAAAACGCTCTTTGCCTCAGCTGGTTTCACTCCATTATCCTTCAGTAACTTCACGGAGACTCAGGCAGAATGTGTGGTCAAGAGGAGTACAGCAAGAGGGTTTCATGTCGAGAAGCGTCAAGAGTCACTTGTGCTATGCTGGCAGCGCCGAGAGCTCATCTCAGCTTCAGCTTGGAGGTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

784

Amino Acids

86.01

Weight (kDa)

5.89

Isoelectric Point (pI)

55.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 420 - 783 3e-93 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 406
Acc36I ACCTGC 1 cut(s) 314
AccB1I GGYRCC 1 cut(s) 1171
AccBSI CCGCTC 1 cut(s) 510
AccII CGCG 4 cut(s) 280, 479, 1313, 1330
AclWI GGATC 4 cut(s) 361, 464, 1009, 1034
AcsI RAATTY 1 cut(s) 1873
AcuI CTGAAG 8 cut(s) 299, 935, 1061, 1352, 1524, 2093, 2197, 2322
AcyI GRCGYC 2 cut(s) 422, 2140
AfaI GTAC 4 cut(s) 1029, 1294, 2033, 2260
AfiI CCNNNNNNNGG 8 cut(s) 332, 617, 618, 983, 1024, 1103, 1371, 1372
AflIII ACRYGT 1 cut(s) 477
AgsI TTSAA 9 cut(s) 11, 196, 539, 643, 699, 1390, 1477, 1790, 2123
AhdI GACNNNNNGTC 1 cut(s) 478
AjiI CACGTC 1 cut(s) 469
AjnI CCWGG 2 cut(s) 976, 1545
AjuI GAANNNNNNNTTGG 2 cut(s) 605, 637
Alw21I GWGCWC 2 cut(s) 1618, 2328
Alw26I GTCTC 6 cut(s) 392, 429, 885, 1515, 2147, 2222
AlwI GGATC 4 cut(s) 361, 464, 1009, 1034
AlwNI CAGNNNCTG 5 cut(s) 445, 847, 1240, 1574, 1868
Ama87I CYCGRG 4 cut(s) 154, 257, 460, 1003
AoxI GGCC 6 cut(s) 342, 677, 1160, 1275, 1623, 2019
ApoI RAATTY 1 cut(s) 1873
ArsI GACNNNNNNTTYG 2 cut(s) 467, 499
AspLEI GCGC 6 cut(s) 280, 282, 969, 1315, 1330, 2319
AspS9I GGNCC 9 cut(s) 254, 303, 608, 677, 1000, 1261, 1623, 1813, 2020
AsuC2I CCSGG 3 cut(s) 429, 1044, 1119
AsuHPI GGTGA 2 cut(s) 800, 1331
AsuII TTCGAA 1 cut(s) 1895
AvaI CYCGRG 4 cut(s) 154, 257, 460, 1003
AvaII GGWCC 6 cut(s) 254, 303, 608, 1000, 1261, 1813
BaeGI GKGCMC 2 cut(s) 666, 1176
BanI GGYRCC 1 cut(s) 1171
BanII GRGCYC 3 cut(s) 161, 264, 2328
BbsI GAAGAC 1 cut(s) 1489
Bbv12I GWGCWC 2 cut(s) 1618, 2328
BbvCI CCTCAGC 2 cut(s) 1137, 2188
BceAI ACGGC 4 cut(s) 214, 285, 329, 432
BciT130I CCWGG 2 cut(s) 978, 1547
BclI TGATCA 1 cut(s) 1576
BcnI CCSGG 3 cut(s) 429, 1044, 1119
BcoDI GTCTC 6 cut(s) 392, 429, 885, 1515, 2147, 2222
BfaI CTAG 1 cut(s) 1890
BfmI CTRYAG 3 cut(s) 233, 1244, 2015
BfoI RGCGCY 2 cut(s) 970, 2320
BfuAI ACCTGC 1 cut(s) 314
BlpI GCTNAGC 1 cut(s) 930
Bme1390I CCNGG 5 cut(s) 429, 978, 1044, 1119, 1547
Bme18I GGWCC 6 cut(s) 254, 303, 608, 1000, 1261, 1813
BmeRI GACNNNNNGTC 1 cut(s) 478
BmeT110I CYCGRG 4 cut(s) 154, 257, 460, 1003
BmgBI CACGTC 1 cut(s) 469
BmgT120I GGNCC 9 cut(s) 254, 303, 608, 677, 1000, 1261, 1623, 1813, 2020
BmiI GGNNCC 8 cut(s) 71, 255, 256, 574, 610, 1002, 1173, 2102
BmrFI CCNGG 5 cut(s) 429, 978, 1044, 1119, 1547
BmrI ACTGGG 3 cut(s) 403, 1305, 1630
BmsI GCATC 5 cut(s) 62, 182, 359, 2047, 2068
BmuI ACTGGG 3 cut(s) 403, 1305, 1630
BpiI GAAGAC 1 cut(s) 1489
BplI GAGNNNNNCTC 2 cut(s) 2315, 2347
BpmI CTGGAG 1 cut(s) 359
Bpu10I CCTNAGC 3 cut(s) 1137, 1774, 2188
Bpu1102I GCTNAGC 1 cut(s) 930
Bpu14I TTCGAA 1 cut(s) 1895
BpuEI CTTGAG 2 cut(s) 551, 1577
BpuMI CCSGG 3 cut(s) 429, 1044, 1119
BsaHI GRCGYC 2 cut(s) 422, 2140
BsaI GGTCTC 1 cut(s) 392
BsaJI CCNNGG 2 cut(s) 976, 1157
BsaWI WCCGGW 4 cut(s) 350, 450, 575, 605
Bsc4I CCNNNNNNNGG 8 cut(s) 332, 617, 618, 983, 1024, 1103, 1371, 1372
Bse118I RCCGGY 3 cut(s) 247, 1162, 1903
Bse1I ACTGG 9 cut(s) 71, 398, 439, 773, 877, 917, 1002, 1300, 1625
Bse3DI GCAATG 1 cut(s) 550
BseBI CCWGG 2 cut(s) 978, 1547
BseDI CCNNGG 2 cut(s) 976, 1157
BseGI GGATG 3 cut(s) 173, 301, 2083
BseLI CCNNNNNNNGG 8 cut(s) 332, 617, 618, 983, 1024, 1103, 1371, 1372
BseMI GCAATG 1 cut(s) 550
BseMII CTCAG 9 cut(s) 854, 896, 944, 1025, 1075, 1151, 2202, 2246, 2346
BseNI ACTGG 9 cut(s) 71, 398, 439, 773, 877, 917, 1002, 1300, 1625
BsePI GCGCGC 1 cut(s) 278
BseRI GAGGAG 7 cut(s) 101, 175, 178, 1397, 1400, 1428, 2269
BseSI GKGCMC 2 cut(s) 666, 1176
BseYI CCCAGC 4 cut(s) 326, 665, 957, 1426
Bsh1236I CGCG 4 cut(s) 280, 479, 1313, 1330
BshFI GGCC 6 cut(s) 344, 679, 1162, 1277, 1625, 2021
BshNI GGYRCC 1 cut(s) 1171
BsiHKAI GWGCWC 2 cut(s) 1618, 2328
BsiHKCI CYCGRG 4 cut(s) 154, 257, 460, 1003
BslFI GGGAC 3 cut(s) 267, 594, 940
BslI CCNNNNNNNGG 8 cut(s) 332, 617, 618, 983, 1024, 1103, 1371, 1372
BsmAI GTCTC 6 cut(s) 392, 429, 885, 1515, 2147, 2222
BsmBI CGTCTC 2 cut(s) 429, 2147
BsmFI GGGAC 3 cut(s) 267, 594, 940
BsmI GAATGC 1 cut(s) 1128
BsnI GGCC 6 cut(s) 344, 679, 1162, 1277, 1625, 2021
Bso31I GGTCTC 1 cut(s) 392
BsoBI CYCGRG 4 cut(s) 154, 257, 460, 1003
Bsp119I TTCGAA 1 cut(s) 1895
Bsp1286I GDGCHC 6 cut(s) 161, 264, 666, 1176, 1618, 2328
Bsp143I GATC 6 cut(s) 353, 456, 565, 1014, 1039, 1576
Bsp1720I GCTNAGC 1 cut(s) 930
BspANI GGCC 6 cut(s) 344, 679, 1162, 1277, 1625, 2021
BspCNI CTCAG 9 cut(s) 853, 895, 943, 1024, 1074, 1150, 2201, 2245, 2345
BspFNI CGCG 4 cut(s) 280, 479, 1313, 1330
BspHI TCATGA 2 cut(s) 864, 1048
BspLI GGNNCC 8 cut(s) 71, 255, 256, 574, 610, 1002, 1173, 2102
BspMAI CTGCAG 2 cut(s) 237, 2019
BspMI ACCTGC 1 cut(s) 314
BspPI GGATC 4 cut(s) 361, 464, 1009, 1034
BspQI GCTCTTC 1 cut(s) 676
BspT104I TTCGAA 1 cut(s) 1895
BspT107I GGYRCC 1 cut(s) 1171
BspTNI GGTCTC 1 cut(s) 392
BsrBI CCGCTC 1 cut(s) 510
BsrDI GCAATG 1 cut(s) 550
BsrFI RCCGGY 3 cut(s) 247, 1162, 1903
BsrI ACTGG 9 cut(s) 71, 398, 439, 773, 877, 917, 1002, 1300, 1625
BssAI RCCGGY 3 cut(s) 247, 1162, 1903
BssECI CCNNGG 2 cut(s) 976, 1157
BssHII GCGCGC 1 cut(s) 278
BssMI GATC 6 cut(s) 353, 456, 565, 1014, 1039, 1576
BssNI GRCGYC 2 cut(s) 422, 2140
Bst2UI CCWGG 2 cut(s) 978, 1547
Bst4CI ACNGT 4 cut(s) 212, 521, 1720, 2131
Bst6I CTCTTC 1 cut(s) 676
BstACI GRCGYC 2 cut(s) 422, 2140
BstAPI GCANNNNNTGC 4 cut(s) 502, 1240, 1255, 2014
BstBI TTCGAA 1 cut(s) 1895
BstC8I GCNNGC 7 cut(s) 280, 311, 377, 1317, 1374, 2019, 2312
BstF5I GGATG 3 cut(s) 173, 301, 2083
BstFNI CGCG 4 cut(s) 280, 479, 1313, 1330
BstH2I RGCGCY 2 cut(s) 970, 2320
BstHHI GCGC 6 cut(s) 280, 282, 969, 1315, 1330, 2319
BstKTI GATC 6 cut(s) 356, 459, 568, 1017, 1042, 1579
BstMAI GTCTC 6 cut(s) 392, 429, 885, 1515, 2147, 2222
BstMBI GATC 6 cut(s) 353, 456, 565, 1014, 1039, 1576
BstNI CCWGG 2 cut(s) 978, 1547
BstSCI CCNGG 5 cut(s) 427, 976, 1042, 1117, 1545
BstSFI CTRYAG 3 cut(s) 233, 1244, 2015
BstSLI GKGCMC 2 cut(s) 666, 1176
BstUI CGCG 4 cut(s) 280, 479, 1313, 1330
BstV2I GAAGAC 1 cut(s) 1489
BstX2I RGATCY 2 cut(s) 456, 1014
BstXI CCANNNNNNTGG 1 cut(s) 1957
BstYI RGATCY 2 cut(s) 456, 1014
BsuRI GGCC 6 cut(s) 344, 679, 1162, 1277, 1625, 2021
BtrI CACGTC 1 cut(s) 469
BtsCI GGATG 3 cut(s) 173, 301, 2083
BtsI GCAGTG 2 cut(s) 1686, 1829
BtsIMutI CAGTG 5 cut(s) 924, 1618, 1686, 1829, 2127
BveI ACCTGC 1 cut(s) 314
Cac8I GCNNGC 7 cut(s) 280, 311, 377, 1317, 1374, 2019, 2312
CaiI CAGNNNCTG 5 cut(s) 445, 847, 1240, 1574, 1868
CciI TCATGA 2 cut(s) 864, 1048
CfoI GCGC 6 cut(s) 280, 282, 969, 1315, 1330, 2319
Cfr10I RCCGGY 3 cut(s) 247, 1162, 1903
Cfr13I GGNCC 9 cut(s) 254, 303, 608, 677, 1000, 1261, 1623, 1813, 2020
CseI GACGC 5 cut(s) 411, 468, 485, 2129, 2276
Csp6I GTAC 4 cut(s) 1028, 1293, 2032, 2259
CspCI CAANNNNNGTGG 2 cut(s) 961, 996
CviAII CATG 6 cut(s) 403, 788, 865, 913, 1049, 2276
CviQI GTAC 4 cut(s) 1028, 1293, 2032, 2259
DpnI GATC 6 cut(s) 355, 458, 567, 1016, 1041, 1578
DpnII GATC 6 cut(s) 353, 456, 565, 1014, 1039, 1576
DrdI GACNNNNNNGTC 1 cut(s) 406
DriI GACNNNNNGTC 1 cut(s) 478
DseDI GACNNNNNNGTC 1 cut(s) 406
Eam1104I CTCTTC 1 cut(s) 676
Eam1105I GACNNNNNGTC 1 cut(s) 478
EarI CTCTTC 1 cut(s) 676
Ecl136II GAGCTC 1 cut(s) 2326
Eco24I GRGCYC 3 cut(s) 161, 264, 2328
Eco31I GGTCTC 1 cut(s) 392
Eco47I GGWCC 6 cut(s) 254, 303, 608, 1000, 1261, 1813
Eco53kI GAGCTC 1 cut(s) 2326
Eco57I CTGAAG 8 cut(s) 299, 935, 1061, 1352, 1524, 2093, 2197, 2322
Eco88I CYCGRG 4 cut(s) 154, 257, 460, 1003
EcoICRI GAGCTC 1 cut(s) 2326
EcoO109I RGGNCCY 2 cut(s) 254, 2020
EcoRII CCWGG 2 cut(s) 976, 1545
EcoT38I GRGCYC 3 cut(s) 161, 264, 2328
Esp3I CGTCTC 2 cut(s) 429, 2147
FaeI CATG 6 cut(s) 406, 791, 868, 916, 1052, 2279
FalI AAGNNNNNCTT 4 cut(s) 20, 52, 2283, 2315
FaqI GGGAC 3 cut(s) 267, 594, 940
FatI CATG 6 cut(s) 402, 787, 864, 912, 1048, 2275
FauI CCCGC 5 cut(s) 356, 368, 503, 541, 1365
FauNDI CATATG 1 cut(s) 141
FbaI TGATCA 1 cut(s) 1576
FokI GGATG 3 cut(s) 160, 308, 2090
FriOI GRGCYC 3 cut(s) 161, 264, 2328
FspBI CTAG 1 cut(s) 1890
GlaI GCGC 6 cut(s) 279, 281, 968, 1314, 1329, 2318
GsaI CCCAGC 4 cut(s) 330, 669, 961, 1430
GsuI CTGGAG 1 cut(s) 359
HaeII RGCGCY 2 cut(s) 970, 2320
HaeIII GGCC 6 cut(s) 344, 679, 1162, 1277, 1625, 2021
HgaI GACGC 5 cut(s) 411, 468, 485, 2129, 2276
HhaI GCGC 6 cut(s) 280, 282, 969, 1315, 1330, 2319
Hin1I GRCGYC 2 cut(s) 422, 2140
Hin1II CATG 6 cut(s) 406, 791, 868, 916, 1052, 2279
Hin6I GCGC 6 cut(s) 278, 280, 967, 1313, 1328, 2317
HinP1I GCGC 6 cut(s) 278, 280, 967, 1313, 1328, 2317
HincII GTYRAC 1 cut(s) 1798
HindII GTYRAC 1 cut(s) 1798
HinfI GANTC 9 cut(s) 322, 805, 844, 1519, 1582, 1897, 2048, 2230, 2294
HpaI GTTAAC 1 cut(s) 1798
HphI GGTGA 2 cut(s) 800, 1331
Hpy166II GTNNAC 4 cut(s) 303, 472, 1798, 2086
Hpy8I GTNNAC 4 cut(s) 303, 472, 1798, 2086
Hpy99I CGWCG 3 cut(s) 470, 479, 484
HpyCH4III ACNGT 4 cut(s) 212, 521, 1720, 2131
HpyCH4IV ACGT 1 cut(s) 468
HpyCH4V TGCA 7 cut(s) 235, 505, 1126, 1402, 1542, 1636, 2017
HpySE526I ACGT 1 cut(s) 468
Hsp92I GRCGYC 2 cut(s) 422, 2140
Hsp92II CATG 6 cut(s) 406, 791, 868, 916, 1052, 2279
HspAI GCGC 6 cut(s) 278, 280, 967, 1313, 1328, 2317
KflI GGGWCCC 1 cut(s) 254
Ksp22I TGATCA 1 cut(s) 1576
KspAI GTTAAC 1 cut(s) 1798
Kzo9I GATC 6 cut(s) 353, 456, 565, 1014, 1039, 1576
LguI GCTCTTC 1 cut(s) 676
LmnI GCTCC 5 cut(s) 88, 453, 594, 891, 937
LweI GCATC 5 cut(s) 62, 182, 359, 2047, 2068
MaeI CTAG 1 cut(s) 1890
MaeII ACGT 1 cut(s) 468
MalI GATC 6 cut(s) 355, 458, 567, 1016, 1041, 1578
MbiI CCGCTC 1 cut(s) 510
MboI GATC 6 cut(s) 353, 456, 565, 1014, 1039, 1576
MboII GAAGA 7 cut(s) 128, 159, 663, 941, 1208, 1465, 1489
MflI RGATCY 2 cut(s) 456, 1014
MhlI GDGCHC 6 cut(s) 161, 264, 666, 1176, 1618, 2328
MluI ACGCGT 1 cut(s) 477
MlyI GAGTC 2 cut(s) 2224, 2303
MmeI TCCRAC 3 cut(s) 366, 1336, 1791
MseI TTAA 5 cut(s) 1728, 1748, 1797, 1935, 2064
MslI CAYNNNNRTG 1 cut(s) 917
MspA1I CMGCKG 7 cut(s) 50, 330, 499, 682, 859, 1372, 2192
MspR9I CCNGG 5 cut(s) 429, 978, 1044, 1119, 1547
Mva1269I GAATGC 1 cut(s) 1128
MvaI CCWGG 2 cut(s) 978, 1547
MvnI CGCG 4 cut(s) 280, 479, 1313, 1330
NciI CCSGG 3 cut(s) 429, 1044, 1119
NdeI CATATG 1 cut(s) 141
NdeII GATC 6 cut(s) 353, 456, 565, 1014, 1039, 1576
NlaIII CATG 6 cut(s) 406, 791, 868, 916, 1052, 2279
NlaIV GGNNCC 8 cut(s) 71, 255, 256, 574, 610, 1002, 1173, 2102
NmeAIII GCCGAG 1 cut(s) 2345
NmuCI GTSAC 6 cut(s) 62, 346, 919, 1979, 1988, 2295
NspV TTCGAA 1 cut(s) 1895
PagI TCATGA 2 cut(s) 864, 1048
PauI GCGCGC 1 cut(s) 278
PciSI GCTCTTC 1 cut(s) 676
PcsI WCGNNNNNNNCGW 2 cut(s) 117, 597
PctI GAATGC 1 cut(s) 1128
PfeI GAWTC 7 cut(s) 322, 805, 844, 1519, 1582, 1897, 2048
PfoI TCCNGGA 1 cut(s) 1117
PleI GAGTC 2 cut(s) 2224, 2302
PpsI GAGTC 2 cut(s) 2224, 2302
PpuMI RGGWCCY 1 cut(s) 254
Psp124BI GAGCTC 1 cut(s) 2328
Psp5II RGGWCCY 1 cut(s) 254
Psp6I CCWGG 2 cut(s) 976, 1545
PspFI CCCAGC 4 cut(s) 326, 665, 957, 1426
PspGI CCWGG 2 cut(s) 976, 1545
PspN4I GGNNCC 8 cut(s) 71, 255, 256, 574, 610, 1002, 1173, 2102
PspPI GGNCC 9 cut(s) 254, 303, 608, 677, 1000, 1261, 1623, 1813, 2020
PspPPI RGGWCCY 1 cut(s) 254
PstI CTGCAG 2 cut(s) 237, 2019
PstNI CAGNNNCTG 5 cut(s) 445, 847, 1240, 1574, 1868
PsuI RGATCY 2 cut(s) 456, 1014
PteI GCGCGC 1 cut(s) 278
PvuII CAGCTG 3 cut(s) 499, 859, 2192
RsaI GTAC 4 cut(s) 1029, 1294, 2033, 2260
RsaNI GTAC 4 cut(s) 1028, 1293, 2032, 2259
RseI CAYNNNNRTG 1 cut(s) 917
SacI GAGCTC 1 cut(s) 2328
SapI GCTCTTC 1 cut(s) 676
SaqAI TTAA 5 cut(s) 1728, 1748, 1797, 1935, 2064
Sau3AI GATC 6 cut(s) 353, 456, 565, 1014, 1039, 1576
Sau96I GGNCC 9 cut(s) 254, 303, 608, 677, 1000, 1261, 1623, 1813, 2020
SchI GAGTC 2 cut(s) 2224, 2303
ScrFI CCNGG 5 cut(s) 429, 978, 1044, 1119, 1547
SduI GDGCHC 6 cut(s) 161, 264, 666, 1176, 1618, 2328
SfaNI GCATC 5 cut(s) 62, 182, 359, 2047, 2068
SfcI CTRYAG 3 cut(s) 233, 1244, 2015
SfuI TTCGAA 1 cut(s) 1895
SgrAI CRCCGGYG 1 cut(s) 247
SinI GGWCC 6 cut(s) 254, 303, 608, 1000, 1261, 1813
SmiMI CAYNNNNRTG 1 cut(s) 917
SmlI CTYRAG 2 cut(s) 530, 1556
SmoI CTYRAG 2 cut(s) 530, 1556
SspI AATATT 1 cut(s) 1034
SspMI CTAG 1 cut(s) 1890
SstI GAGCTC 1 cut(s) 2328
StyD4I CCNGG 5 cut(s) 427, 976, 1042, 1117, 1545
TaaI ACNGT 4 cut(s) 212, 521, 1720, 2131
TaiI ACGT 1 cut(s) 471
TaqI TCGA 6 cut(s) 320, 564, 582, 1895, 1927, 2280
TatI WGTACW 1 cut(s) 2258
TfiI GAWTC 7 cut(s) 322, 805, 844, 1519, 1582, 1897, 2048
Tru1I TTAA 5 cut(s) 1728, 1748, 1797, 1935, 2064
Tru9I TTAA 5 cut(s) 1728, 1748, 1797, 1935, 2064
TscAI CASTG 5 cut(s) 924, 1625, 1693, 1836, 2134
TseFI GTSAC 6 cut(s) 62, 346, 919, 1979, 1988, 2295
Tsp45I GTSAC 6 cut(s) 62, 346, 919, 1979, 1988, 2295
TspDTI ATGAA 8 cut(s) 85, 128, 728, 853, 881, 901, 1433, 2264
TspGWI ACGGA 1 cut(s) 2240
TspRI CASTG 5 cut(s) 924, 1625, 1693, 1836, 2134
VpaK11BI GGWCC 6 cut(s) 254, 303, 608, 1000, 1261, 1813
XapI RAATTY 1 cut(s) 1873
XcmI CCANNNNNNNNNTGG 3 cut(s) 334, 977, 1183
XspI CTAG 1 cut(s) 1890
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.