FvH4_5g20190

50S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0013276
Physical Location & Seq
Reverse (-)
11916971 .. 11919443
2473 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g20190.t2

Sequence Viewer

Length: 702 bp
ATGGGCTCCAAGGTCCTTCCTCAGGCTCTGCTAACAATCCCAAGAAACCCTAACCACTACGCGCCGGTAAAGCTCGGGTTGTCTCGTCGCCCTTCCTTCTGTTCCACTGGCGTTTCGCTCTGCAAGGCTTCTTCGAGCTCAGTCAGGTCCAAATTCAGCCCCATTGTCGCCGCAAAGTCCAGACGGGACTTTGCTGTGAGAGCCGAGTCGGAAGGTGAGGTGGGAGCCACCGAGAATGAAGAAGCTGTTGGTGAACCGGCTTCAGAGGCCGTAGTGGACGCCGAAGCAGGAGCAGCTCTGGAGACTGAGCCTGAGCCTGAAGAGAAGAAGCCGCCGCGGAAGCCTCGTATCAAGCTCGGCGATGTCATGGGGATACTAAATAGCCGGGCAATTGATGCGGCAGAGAAGGAAAGGCCAGTTCCGGACATTAGGAGTGGAGATGTTGTGCAAATTAAGCTGGAAGTTCCGGAAAACAAGCGTAGGCTGTCTATCTATAAAGGTATCGTTATTTCAAAGCAAAATGCTGGCATTCATACAACATTTCGCATTCGGAGGATTATTGCTGGTGTAGGGGTTGAGATTGTTTTCCCTCTATACTCACCAAACATCAAGGAGTTGAAAGTACTTAGTCACAGGAAGGTCAGAAGGGCAAGGCTATACTATTTGAGAGACAAACTTCCTAGGTTTTCGACTTTCAAGTGA

Protein Analysis

234

Amino Acids

25.73

Weight (kDa)

10.0

Isoelectric Point (pI)

56.99

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013276)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G17560 AT5G47190
fragaria_vesca FvH4_5g20190 FvH4_5g20190
malus_domestica MD04G1051500.v1.1 MD06G1048700.v1.1
prunus_persica Prupe.5G058800_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0205151
rosa_laevigata RLG00000003395
rosa_multiflora Rmu_sc0001072.1_g000005 Rmu_sc0009349.1_g000012
rosa_roxburghii Rroxscaffold_3G00252540
rosa_rugosa Rorug07G0088500
rosa_samantha Rh7AG219400 Rh7BG215800 Rh7CG232400 Rh7DG226700
rosa_wichuraiana Rw7G018980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 62, 337
AccIII TCCGGA 2 cut(s) 421, 466
AciI CCGC 5 cut(s) 171, 332, 335, 337, 398
AcsI RAATTY 1 cut(s) 152
AcuI CTGAAG 2 cut(s) 246, 339
AcyI GRCGYC 1 cut(s) 279
AfaI GTAC 1 cut(s) 624
AfiI CCNNNNNNNGG 1 cut(s) 22
AgsI TTSAA 3 cut(s) 513, 619, 697
AjuI GAANNNNNNNTTGG 2 cut(s) 231, 263
AluBI AGCT 6 cut(s) 73, 138, 245, 296, 355, 457
AluI AGCT 6 cut(s) 73, 138, 245, 296, 355, 457
Alw21I GWGCWC 1 cut(s) 140
Alw26I GTCTC 3 cut(s) 87, 296, 663
AlwNI CAGNNNCTG 1 cut(s) 28
Ama87I CYCGRG 1 cut(s) 74
Aor13HI TCCGGA 2 cut(s) 421, 466
AoxI GGCC 2 cut(s) 267, 413
ApeKI GCWGC 1 cut(s) 293
ApoI RAATTY 1 cut(s) 152
AspA2I CCTAGG 1 cut(s) 680
AspLEI GCGC 1 cut(s) 64
AspS9I GGNCC 2 cut(s) 13, 147
AsuC2I CCSGG 1 cut(s) 386
AsuHPI GGTGA 3 cut(s) 227, 263, 591
AvaI CYCGRG 1 cut(s) 74
AvaII GGWCC 2 cut(s) 13, 147
AvrII CCTAGG 1 cut(s) 680
AxyI CCTNAGG 1 cut(s) 21
BanII GRGCYC 2 cut(s) 8, 140
Bbv12I GWGCWC 1 cut(s) 140
BbvI GCAGC 1 cut(s) 305
BceAI ACGGC 1 cut(s) 254
BciVI GTATCC 1 cut(s) 366
BcnI CCSGG 1 cut(s) 386
BcoDI GTCTC 3 cut(s) 87, 296, 663
BfaI CTAG 1 cut(s) 681
BfuI GTATCC 1 cut(s) 366
BisI GCNGC 5 cut(s) 171, 294, 332, 335, 399
BlnI CCTAGG 1 cut(s) 680
BlsI GCNGC 5 cut(s) 172, 295, 333, 336, 400
BmcAI AGTACT 1 cut(s) 624
Bme1390I CCNGG 1 cut(s) 386
Bme18I GGWCC 2 cut(s) 13, 147
BmeT110I CYCGRG 1 cut(s) 74
BmgT120I GGNCC 2 cut(s) 13, 147
BmiI GGNNCC 2 cut(s) 7, 226
BmrFI CCNGG 1 cut(s) 386
BmsI GCATC 1 cut(s) 385
BpmI CTGGAG 1 cut(s) 320
Bpu10I CCTNAGC 1 cut(s) 312
BpuMI CCSGG 1 cut(s) 386
BsaHI GRCGYC 1 cut(s) 279
BsaJI CCNNGG 3 cut(s) 9, 335, 680
BsaWI WCCGGW 2 cut(s) 421, 466
BsaXI ACNNNNNCTCC 2 cut(s) 429, 459
Bsc4I CCNNNNNNNGG 1 cut(s) 22
Bse118I RCCGGY 2 cut(s) 64, 256
Bse1I ACTGG 2 cut(s) 112, 416
Bse21I CCTNAGG 1 cut(s) 21
BseAI TCCGGA 2 cut(s) 421, 466
BseDI CCNNGG 3 cut(s) 9, 335, 680
BseLI CCNNNNNNNGG 1 cut(s) 22
BseMII CTCAG 4 cut(s) 35, 153, 297, 303
BseNI ACTGG 2 cut(s) 112, 416
BseXI GCAGC 1 cut(s) 305
Bsh1236I CGCG 2 cut(s) 62, 337
BshFI GGCC 2 cut(s) 269, 415
BsiHKAI GWGCWC 1 cut(s) 140
BsiHKCI CYCGRG 1 cut(s) 74
BsiSI CCGG 5 cut(s) 65, 257, 385, 422, 467
BslFI GGGAC 1 cut(s) 200
BslI CCNNNNNNNGG 1 cut(s) 22
BsmAI GTCTC 3 cut(s) 87, 296, 663
BsmFI GGGAC 1 cut(s) 200
BsmI GAATGC 2 cut(s) 528, 546
BsnI GGCC 2 cut(s) 269, 415
BsoBI CYCGRG 1 cut(s) 74
Bsp1286I GDGCHC 2 cut(s) 8, 140
Bsp13I TCCGGA 2 cut(s) 421, 466
BspACI CCGC 5 cut(s) 171, 332, 335, 337, 398
BspANI GGCC 2 cut(s) 269, 415
BspCNI CTCAG 4 cut(s) 34, 152, 298, 304
BspEI TCCGGA 2 cut(s) 421, 466
BspFNI CGCG 2 cut(s) 62, 337
BspLI GGNNCC 2 cut(s) 7, 226
BsrFI RCCGGY 2 cut(s) 64, 256
BsrI ACTGG 2 cut(s) 112, 416
BssAI RCCGGY 2 cut(s) 64, 256
BssECI CCNNGG 3 cut(s) 9, 335, 680
BssNI GRCGYC 1 cut(s) 279
BssT1I CCWWGG 2 cut(s) 9, 680
Bst6I CTCTTC 1 cut(s) 315
BstACI GRCGYC 1 cut(s) 279
BstAPI GCANNNNNTGC 1 cut(s) 395
BstC8I GCNNGC 1 cut(s) 526
BstDEI CTNAG 5 cut(s) 21, 139, 306, 312, 626
BstDSI CCRYGG 1 cut(s) 335
BstENI CCTNNNNNAGG 1 cut(s) 20
BstFNI CGCG 2 cut(s) 62, 337
BstHHI GCGC 1 cut(s) 64
BstMAI GTCTC 3 cut(s) 87, 296, 663
BstMWI GCNNNNNNNGC 7 cut(s) 70, 200, 266, 293, 340, 395, 454
BstSCI CCNGG 1 cut(s) 384
BstUI CGCG 2 cut(s) 62, 337
BstV1I GCAGC 1 cut(s) 305
Bsu36I CCTNAGG 1 cut(s) 21
BsuI GTATCC 1 cut(s) 366
BsuRI GGCC 2 cut(s) 269, 415
BtgI CCRYGG 1 cut(s) 335
BtgZI GCGATG 1 cut(s) 375
BtsIMutI CAGTG 1 cut(s) 105
Cac8I GCNNGC 1 cut(s) 526
CaiI CAGNNNCTG 1 cut(s) 28
CfoI GCGC 1 cut(s) 64
Cfr10I RCCGGY 2 cut(s) 64, 256
Cfr13I GGNCC 2 cut(s) 13, 147
Cfr42I CCGCGG 1 cut(s) 338
CseI GACGC 1 cut(s) 287
Csp6I GTAC 1 cut(s) 623
CviAII CATG 1 cut(s) 367
CviQI GTAC 1 cut(s) 623
DdeI CTNAG 5 cut(s) 21, 139, 306, 312, 626
Eam1104I CTCTTC 1 cut(s) 315
EarI CTCTTC 1 cut(s) 315
Ecl136II GAGCTC 1 cut(s) 138
Eco130I CCWWGG 2 cut(s) 9, 680
Eco24I GRGCYC 2 cut(s) 8, 140
Eco47I GGWCC 2 cut(s) 13, 147
Eco53kI GAGCTC 1 cut(s) 138
Eco57I CTGAAG 2 cut(s) 246, 339
Eco81I CCTNAGG 1 cut(s) 21
Eco88I CYCGRG 1 cut(s) 74
EcoICRI GAGCTC 1 cut(s) 138
EcoNI CCTNNNNNAGG 1 cut(s) 20
EcoO109I RGGNCCY 1 cut(s) 13
EcoT14I CCWWGG 2 cut(s) 9, 680
EcoT38I GRGCYC 2 cut(s) 8, 140
ErhI CCWWGG 2 cut(s) 9, 680
FaeI CATG 1 cut(s) 370
FaiI YATR 5 cut(s) 368, 495, 534, 595, 658
FaqI GGGAC 1 cut(s) 200
FatI CATG 1 cut(s) 366
Fnu4HI GCNGC 5 cut(s) 171, 294, 332, 335, 399
FriOI GRGCYC 2 cut(s) 8, 140
Fsp4HI GCNGC 5 cut(s) 171, 294, 332, 335, 399
FspBI CTAG 1 cut(s) 681
GlaI GCGC 1 cut(s) 63
GluI GCNGC 5 cut(s) 171, 294, 332, 335, 399
GsuI CTGGAG 1 cut(s) 320
HaeIII GGCC 2 cut(s) 269, 415
HapII CCGG 5 cut(s) 65, 257, 385, 422, 467
HgaI GACGC 1 cut(s) 287
HhaI GCGC 1 cut(s) 64
Hin1I GRCGYC 1 cut(s) 279
Hin1II CATG 1 cut(s) 370
Hin6I GCGC 1 cut(s) 62
HinP1I GCGC 1 cut(s) 62
HinfI GANTC 1 cut(s) 206
HpaII CCGG 5 cut(s) 65, 257, 385, 422, 467
HphI GGTGA 3 cut(s) 227, 263, 591
Hpy166II GTNNAC 2 cut(s) 254, 277
Hpy188I TCNGA 4 cut(s) 211, 265, 552, 644
Hpy188III TCNNGA 4 cut(s) 180, 299, 422, 467
Hpy8I GTNNAC 2 cut(s) 254, 277
Hpy99I CGWCG 1 cut(s) 90
HpyAV CCTTC 7 cut(s) 26, 102, 106, 206, 400, 631, 639
HpyCH4V TGCA 2 cut(s) 123, 448
HpyF10VI GCNNNNNNNGC 7 cut(s) 70, 200, 266, 293, 340, 395, 454
HpyF3I CTNAG 5 cut(s) 21, 139, 306, 312, 626
Hsp92I GRCGYC 1 cut(s) 279
Hsp92II CATG 1 cut(s) 370
HspAI GCGC 1 cut(s) 62
Kpn2I TCCGGA 2 cut(s) 421, 466
KspI CCGCGG 1 cut(s) 338
LmnI GCTCC 3 cut(s) 11, 224, 290
Lsp1109I GCAGC 1 cut(s) 305
LweI GCATC 1 cut(s) 385
MaeI CTAG 1 cut(s) 681
MaeIII GTNAC 1 cut(s) 629
MboII GAAGA 4 cut(s) 123, 251, 332, 337
MfeI CAATTG 1 cut(s) 390
MhlI GDGCHC 2 cut(s) 8, 140
MluCI AATT 3 cut(s) 152, 390, 450
MlyI GAGTC 1 cut(s) 215
MmeI TCCRAC 1 cut(s) 189
MnlI CCTC 6 cut(s) 30, 211, 259, 354, 546, 600
MroI TCCGGA 2 cut(s) 421, 466
MseI TTAA 1 cut(s) 453
MspA1I CMGCKG 1 cut(s) 337
MspI CCGG 5 cut(s) 65, 257, 385, 422, 467
MspR9I CCNGG 1 cut(s) 386
MunI CAATTG 1 cut(s) 390
Mva1269I GAATGC 2 cut(s) 528, 546
MvnI CGCG 2 cut(s) 62, 337
MwoI GCNNNNNNNGC 7 cut(s) 70, 200, 266, 293, 340, 395, 454
NciI CCSGG 1 cut(s) 386
NlaIII CATG 1 cut(s) 370
NlaIV GGNNCC 2 cut(s) 7, 226
NmeAIII GCCGAG 2 cut(s) 229, 336
NmuCI GTSAC 1 cut(s) 629
PctI GAATGC 2 cut(s) 528, 546
PkrI GCNGC 5 cut(s) 172, 295, 333, 336, 400
PleI GAGTC 1 cut(s) 214
PpsI GAGTC 1 cut(s) 214
PpuMI RGGWCCY 1 cut(s) 13
Psp124BI GAGCTC 1 cut(s) 140
Psp5II RGGWCCY 1 cut(s) 13
PspN4I GGNNCC 2 cut(s) 7, 226
PspPI GGNCC 2 cut(s) 13, 147
PspPPI RGGWCCY 1 cut(s) 13
PstNI CAGNNNCTG 1 cut(s) 28
RsaI GTAC 1 cut(s) 624
RsaNI GTAC 1 cut(s) 623
SacI GAGCTC 1 cut(s) 140
SacII CCGCGG 1 cut(s) 338
SaqAI TTAA 1 cut(s) 453
SatI GCNGC 5 cut(s) 171, 294, 332, 335, 399
Sau96I GGNCC 2 cut(s) 13, 147
ScaI AGTACT 1 cut(s) 624
SchI GAGTC 1 cut(s) 215
ScrFI CCNGG 1 cut(s) 386
SduI GDGCHC 2 cut(s) 8, 140
SfaNI GCATC 1 cut(s) 385
Sfr303I CCGCGG 1 cut(s) 338
SgrBI CCGCGG 1 cut(s) 338
SinI GGWCC 2 cut(s) 13, 147
Sse9I AATT 3 cut(s) 152, 390, 450
SsiI CCGC 5 cut(s) 171, 332, 335, 337, 398
SspMI CTAG 1 cut(s) 681
SstI GAGCTC 1 cut(s) 140
StyD4I CCNGG 1 cut(s) 384
StyI CCWWGG 2 cut(s) 9, 680
TaqI TCGA 2 cut(s) 134, 689
TasI AATT 3 cut(s) 152, 390, 450
TatI WGTACW 1 cut(s) 622
TauI GCSGC 4 cut(s) 173, 334, 337, 401
Tru1I TTAA 1 cut(s) 453
Tru9I TTAA 1 cut(s) 453
TscAI CASTG 1 cut(s) 112
TseFI GTSAC 1 cut(s) 629
TseI GCWGC 1 cut(s) 293
Tsp45I GTSAC 1 cut(s) 629
TspDTI ATGAA 2 cut(s) 252, 521
TspRI CASTG 1 cut(s) 112
VpaK11BI GGWCC 2 cut(s) 13, 147
XagI CCTNNNNNAGG 1 cut(s) 20
XapI RAATTY 1 cut(s) 152
XmaJI CCTAGG 1 cut(s) 680
XspI CTAG 1 cut(s) 681
ZrmI AGTACT 1 cut(s) 624
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.