Prupe.5G058800_v2.0.a1

50S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0013276
Physical Location & Seq
Reverse (-)
6344884 .. 6346879
1996 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G058800.1

Sequence Viewer

Length: 846 bp
ATGAGGCTACCTCTGTCGTCGTCCTATCCACCAATCCATCATCCATCATCCATCCACACTTCCATTACCTCTACTCTCTCTCTCTCTCTCTCTCTCTCAATCTTCATTTCCCAGAACCCTAATTCCCAAATCATGGCCTCCAAAGCCCTGCCTCAGGCTCTGATAACAATGCCAAGAAACCCTACCCATCACCCGCCGGTAAAGCTTGGGTTTTCCGCTTCCTTCTCTCGTCGTTCTTCTTCTAATTCCGCCCGGGTCTCGCTCTCTAGGGTTTCGTCGAGCTCATTTGGGTCCAGCTTCTGTCCCATCGTCGCTGCAATGTCGAGACTGGACTTTGTTGTGAGAGCAGATTCGAGCACTGAAGGTGAAGCTGAAGCAGTTGGAAGTGAAGAGGCCTCGGAGGCCATCGTAGAAGCTGAAGCTGAACCAGTTGTGGAGTCTGAGCCTGAAGAGCCTGAGCCTGAAGAGCCGAAGACGCCCAGAAAGTCTCGGGTTAAGCTTGGAGACATAATGGGGATATTAAACAAGAGGGCAGTTGAGGCAGCAGTGCAAGAAAGGCCAGTTCCGGACATTAGAACTGGAGACGTTGTGCAAATCAAACTGGAAGTTCCTGAAAATAAGCGTAGGTTGTCTGTTTATAAAGGCATTGTCATTTCAAGACAAAATGCTGGCATTCACACAACCATTCGCATTCGGAGGATTATTGCTGGAATAGGGGTTGAGATTGTGTTCCCGCTGTACTCACCAAACATTAAGGAGCTAAAGGTACTGAGCCACAGGAAGGTTAGGAGGGCAAGGCTGTACTATTTGAGAGACAAGCTTCCAAGGTTCTCAACTTTCAAATGA

Protein Analysis

282

Amino Acids

31.01

Weight (kDa)

9.94

Isoelectric Point (pI)

61.83

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013276)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G17560 AT5G47190
fragaria_vesca FvH4_5g20190 FvH4_5g20190
malus_domestica MD04G1051500.v1.1 MD06G1048700.v1.1
prunus_persica Prupe.5G058800_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0205151
rosa_laevigata RLG00000003395
rosa_multiflora Rmu_sc0001072.1_g000005 Rmu_sc0009349.1_g000012
rosa_roxburghii Rroxscaffold_3G00252540
rosa_rugosa Rorug07G0088500
rosa_samantha Rh7AG219400 Rh7BG215800 Rh7CG232400 Rh7DG226700
rosa_wichuraiana Rw7G018980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 639
AccB7I CCANNNNNTGG 1 cut(s) 133
AccIII TCCGGA 1 cut(s) 565
AciI CCGC 4 cut(s) 194, 216, 249, 734
AcuI CTGAAG 5 cut(s) 381, 393, 438, 468, 483
AcyI GRCGYC 1 cut(s) 476
AfaI GTAC 3 cut(s) 740, 768, 803
AfiI CCNNNNNNNGG 3 cut(s) 133, 154, 781
AgsI TTSAA 2 cut(s) 657, 841
AloI GAACNNNNNNTCC 2 cut(s) 107, 139
AluBI AGCT 9 cut(s) 205, 282, 297, 371, 416, 422, 499, 760, 820
AluI AGCT 9 cut(s) 205, 282, 297, 371, 416, 422, 499, 760, 820
Alw21I GWGCWC 2 cut(s) 284, 359
Alw26I GTCTC 6 cut(s) 262, 319, 492, 498, 576, 807
AlwNI CAGNNNCTG 2 cut(s) 160, 300
Ama87I CYCGRG 2 cut(s) 252, 489
Aor13HI TCCGGA 1 cut(s) 565
AoxI GGCC 4 cut(s) 135, 393, 402, 557
ApeKI GCWGC 2 cut(s) 314, 542
AspS9I GGNCC 1 cut(s) 291
AsuC2I CCSGG 2 cut(s) 253, 254
AsuHPI GGTGA 3 cut(s) 182, 377, 735
AvaI CYCGRG 2 cut(s) 252, 489
AvaII GGWCC 1 cut(s) 291
AxyI CCTNAGG 1 cut(s) 153
BanII GRGCYC 1 cut(s) 284
BbsI GAAGAC 1 cut(s) 479
Bbv12I GWGCWC 2 cut(s) 284, 359
BbvI GCAGC 2 cut(s) 301, 554
BccI CCATC 6 cut(s) 45, 52, 59, 195, 314, 413
BcnI CCSGG 2 cut(s) 253, 254
BcoDI GTCTC 6 cut(s) 262, 319, 492, 498, 576, 807
BfaI CTAG 1 cut(s) 267
BglI GCCNNNNNGGC 1 cut(s) 401
BisI GCNGC 2 cut(s) 315, 543
BlsI GCNGC 2 cut(s) 316, 544
Bme1390I CCNGG 2 cut(s) 253, 254
Bme18I GGWCC 1 cut(s) 291
BmeT110I CYCGRG 2 cut(s) 252, 489
BmgT120I GGNCC 1 cut(s) 291
BmiI GGNNCC 1 cut(s) 292
BmrFI CCNGG 2 cut(s) 253, 254
BpiI GAAGAC 1 cut(s) 479
BplI GAGNNNNNCTC 1 cut(s) 27
BpmI CTGGAG 1 cut(s) 600
Bpu10I CCTNAGC 1 cut(s) 456
BpuMI CCSGG 2 cut(s) 253, 254
BsaHI GRCGYC 1 cut(s) 476
BsaI GGTCTC 1 cut(s) 262
BsaJI CCNNGG 3 cut(s) 252, 396, 824
BsaWI WCCGGW 1 cut(s) 565
BsaXI ACNNNNNCTCC 2 cut(s) 573, 603
Bsc4I CCNNNNNNNGG 3 cut(s) 133, 154, 781
Bse118I RCCGGY 1 cut(s) 196
Bse1I ACTGG 5 cut(s) 333, 428, 560, 583, 606
Bse21I CCTNAGG 1 cut(s) 153
Bse3DI GCAATG 1 cut(s) 324
BseAI TCCGGA 1 cut(s) 565
BseDI CCNNGG 3 cut(s) 252, 396, 824
BseGI GGATG 3 cut(s) 40, 47, 51
BseLI CCNNNNNNNGG 3 cut(s) 133, 154, 781
BseMI GCAATG 1 cut(s) 324
BseMII CTCAG 4 cut(s) 167, 432, 447, 761
BseNI ACTGG 5 cut(s) 333, 428, 560, 583, 606
BseXI GCAGC 2 cut(s) 301, 554
BshFI GGCC 4 cut(s) 137, 395, 404, 559
BsiHKAI GWGCWC 2 cut(s) 284, 359
BsiHKCI CYCGRG 2 cut(s) 252, 489
BsiSI CCGG 3 cut(s) 197, 253, 566
BslFI GGGAC 1 cut(s) 288
BslI CCNNNNNNNGG 3 cut(s) 133, 154, 781
BsmAI GTCTC 6 cut(s) 262, 319, 492, 498, 576, 807
BsmBI CGTCTC 1 cut(s) 576
BsmFI GGGAC 1 cut(s) 288
BsmI GAATGC 2 cut(s) 672, 690
BsnI GGCC 4 cut(s) 137, 395, 404, 559
Bso31I GGTCTC 1 cut(s) 262
BsoBI CYCGRG 2 cut(s) 252, 489
Bsp1286I GDGCHC 2 cut(s) 284, 359
Bsp13I TCCGGA 1 cut(s) 565
BspACI CCGC 4 cut(s) 194, 216, 249, 734
BspANI GGCC 4 cut(s) 137, 395, 404, 559
BspCNI CTCAG 4 cut(s) 166, 433, 448, 762
BspEI TCCGGA 1 cut(s) 565
BspLI GGNNCC 1 cut(s) 292
BspQI GCTCTTC 2 cut(s) 444, 459
BspTNI GGTCTC 1 cut(s) 262
BsrDI GCAATG 1 cut(s) 324
BsrFI RCCGGY 1 cut(s) 196
BsrI ACTGG 5 cut(s) 333, 428, 560, 583, 606
BssAI RCCGGY 1 cut(s) 196
BssECI CCNNGG 3 cut(s) 252, 396, 824
BssNI GRCGYC 1 cut(s) 476
BssT1I CCWWGG 1 cut(s) 824
Bst6I CTCTTC 3 cut(s) 384, 444, 459
BstACI GRCGYC 1 cut(s) 476
BstC8I GCNNGC 1 cut(s) 670
BstDEI CTNAG 4 cut(s) 153, 441, 456, 770
BstENI CCTNNNNNAGG 1 cut(s) 152
BstF5I GGATG 3 cut(s) 40, 47, 51
BstMAI GTCTC 6 cut(s) 262, 319, 492, 498, 576, 807
BstMWI GCNNNNNNNGC 8 cut(s) 143, 202, 401, 451, 466, 475, 539, 556
BstSCI CCNGG 2 cut(s) 251, 252
BstV1I GCAGC 2 cut(s) 301, 554
BstV2I GAAGAC 1 cut(s) 479
Bsu36I CCTNAGG 1 cut(s) 153
BsuRI GGCC 4 cut(s) 137, 395, 404, 559
BtsCI GGATG 3 cut(s) 40, 47, 51
BtsI GCAGTG 1 cut(s) 552
BtsIMutI CAGTG 2 cut(s) 357, 552
Cac8I GCNNGC 1 cut(s) 670
CaiI CAGNNNCTG 2 cut(s) 160, 300
Cfr10I RCCGGY 1 cut(s) 196
Cfr13I GGNCC 1 cut(s) 291
Cfr9I CCCGGG 1 cut(s) 252
CseI GACGC 1 cut(s) 484
Csp6I GTAC 3 cut(s) 739, 767, 802
CviAII CATG 1 cut(s) 133
CviQI GTAC 3 cut(s) 739, 767, 802
DdeI CTNAG 4 cut(s) 153, 441, 456, 770
Eam1104I CTCTTC 3 cut(s) 384, 444, 459
EarI CTCTTC 3 cut(s) 384, 444, 459
EciI GGCGGA 1 cut(s) 238
Ecl136II GAGCTC 1 cut(s) 282
Eco130I CCWWGG 1 cut(s) 824
Eco147I AGGCCT 1 cut(s) 395
Eco24I GRGCYC 1 cut(s) 284
Eco31I GGTCTC 1 cut(s) 262
Eco47I GGWCC 1 cut(s) 291
Eco53kI GAGCTC 1 cut(s) 282
Eco57I CTGAAG 5 cut(s) 381, 393, 438, 468, 483
Eco81I CCTNAGG 1 cut(s) 153
Eco88I CYCGRG 2 cut(s) 252, 489
EcoICRI GAGCTC 1 cut(s) 282
EcoNI CCTNNNNNAGG 1 cut(s) 152
EcoT14I CCWWGG 1 cut(s) 824
EcoT38I GRGCYC 1 cut(s) 284
ErhI CCWWGG 1 cut(s) 824
Esp3I CGTCTC 1 cut(s) 576
FaeI CATG 1 cut(s) 136
FaiI YATR 3 cut(s) 134, 509, 639
FaqI GGGAC 1 cut(s) 288
FatI CATG 1 cut(s) 132
FauI CCCGC 2 cut(s) 201, 741
Fnu4HI GCNGC 2 cut(s) 315, 543
FokI GGATG 3 cut(s) 27, 34, 38
FriOI GRGCYC 1 cut(s) 284
Fsp4HI GCNGC 2 cut(s) 315, 543
FspBI CTAG 1 cut(s) 267
GluI GCNGC 2 cut(s) 315, 543
GsuI CTGGAG 1 cut(s) 600
HaeIII GGCC 4 cut(s) 137, 395, 404, 559
HapII CCGG 3 cut(s) 197, 253, 566
HgaI GACGC 1 cut(s) 484
Hin1I GRCGYC 1 cut(s) 476
Hin1II CATG 1 cut(s) 136
HindIII AAGCTT 3 cut(s) 203, 497, 818
HinfI GANTC 2 cut(s) 350, 437
HpaII CCGG 3 cut(s) 197, 253, 566
HphI GGTGA 3 cut(s) 182, 377, 735
Hpy188I TCNGA 4 cut(s) 162, 400, 442, 696
Hpy188III TCNNGA 4 cut(s) 324, 566, 611, 657
Hpy99I CGWCG 4 cut(s) 22, 234, 280, 314
HpyAV CCTTC 3 cut(s) 232, 356, 775
HpyCH4IV ACGT 1 cut(s) 585
HpyCH4V TGCA 3 cut(s) 317, 550, 592
HpyF10VI GCNNNNNNNGC 8 cut(s) 143, 202, 401, 451, 466, 475, 539, 556
HpyF3I CTNAG 4 cut(s) 153, 441, 456, 770
HpySE526I ACGT 1 cut(s) 585
Hsp92I GRCGYC 1 cut(s) 476
Hsp92II CATG 1 cut(s) 136
Kpn2I TCCGGA 1 cut(s) 565
LguI GCTCTTC 2 cut(s) 444, 459
LmnI GCTCC 1 cut(s) 757
Lsp1109I GCAGC 2 cut(s) 301, 554
MaeI CTAG 1 cut(s) 267
MaeII ACGT 1 cut(s) 585
MboII GAAGA 7 cut(s) 94, 228, 231, 401, 461, 476, 484
MhlI GDGCHC 2 cut(s) 284, 359
MluCI AATT 2 cut(s) 121, 244
MlyI GAGTC 1 cut(s) 446
MmeI TCCRAC 1 cut(s) 361
MroI TCCGGA 1 cut(s) 565
MseI TTAA 3 cut(s) 495, 521, 753
MspA1I CMGCKG 1 cut(s) 736
MspI CCGG 3 cut(s) 197, 253, 566
MspR9I CCNGG 2 cut(s) 253, 254
Mva1269I GAATGC 2 cut(s) 672, 690
MwoI GCNNNNNNNGC 8 cut(s) 143, 202, 401, 451, 466, 475, 539, 556
NciI CCSGG 2 cut(s) 253, 254
NlaIII CATG 1 cut(s) 136
NlaIV GGNNCC 1 cut(s) 292
PceI AGGCCT 1 cut(s) 395
PciSI GCTCTTC 2 cut(s) 444, 459
PctI GAATGC 2 cut(s) 672, 690
PfeI GAWTC 1 cut(s) 350
PflMI CCANNNNNTGG 1 cut(s) 133
PkrI GCNGC 2 cut(s) 316, 544
PleI GAGTC 1 cut(s) 445
PpsI GAGTC 1 cut(s) 445
PsiI TTATAA 1 cut(s) 639
Psp124BI GAGCTC 1 cut(s) 284
PspN4I GGNNCC 1 cut(s) 292
PspPI GGNCC 1 cut(s) 291
PstNI CAGNNNCTG 2 cut(s) 160, 300
RsaI GTAC 3 cut(s) 740, 768, 803
RsaNI GTAC 3 cut(s) 739, 767, 802
SacI GAGCTC 1 cut(s) 284
SapI GCTCTTC 2 cut(s) 444, 459
SaqAI TTAA 3 cut(s) 495, 521, 753
SatI GCNGC 2 cut(s) 315, 543
Sau96I GGNCC 1 cut(s) 291
SchI GAGTC 1 cut(s) 446
ScrFI CCNGG 2 cut(s) 253, 254
SduI GDGCHC 2 cut(s) 284, 359
SfiI GGCCNNNNNGGCC 1 cut(s) 401
SinI GGWCC 1 cut(s) 291
SmaI CCCGGG 1 cut(s) 254
Sse9I AATT 2 cut(s) 121, 244
SseBI AGGCCT 1 cut(s) 395
SsiI CCGC 4 cut(s) 194, 216, 249, 734
SspMI CTAG 1 cut(s) 267
SstI GAGCTC 1 cut(s) 284
StuI AGGCCT 1 cut(s) 395
StyD4I CCNGG 2 cut(s) 251, 252
StyI CCWWGG 1 cut(s) 824
TaiI ACGT 1 cut(s) 588
TaqI TCGA 3 cut(s) 278, 323, 353
TasI AATT 2 cut(s) 121, 244
TatI WGTACW 2 cut(s) 738, 801
TfiI GAWTC 1 cut(s) 350
Tru1I TTAA 3 cut(s) 495, 521, 753
Tru9I TTAA 3 cut(s) 495, 521, 753
TscAI CASTG 2 cut(s) 364, 552
TseI GCWGC 2 cut(s) 314, 542
TspDTI ATGAA 1 cut(s) 94
TspMI CCCGGG 1 cut(s) 252
TspRI CASTG 2 cut(s) 364, 552
Van91I CCANNNNNTGG 1 cut(s) 133
VpaK11BI GGWCC 1 cut(s) 291
XagI CCTNNNNNAGG 1 cut(s) 152
XmaI CCCGGG 1 cut(s) 252
XspI CTAG 1 cut(s) 267
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.