Rh7DG226700

50S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0013276
Physical Location & Seq
Forward (+)
22149116 .. 22270278
121163 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG226700.1

Sequence Viewer

Length: 708 bp
ATGGGCTCCAAAGTCCTTCCTCAGGCTCTGCTAACAATCCCAAGAAACCCAAACCACTACGCACCGGTAAAGCTCGGGTTTTCAGCCGCCGTGTCTCGTCGGTCTTTCTTCAATTCCACTGGGGTTTCGCTTTCCAGGGCTTCATCGAGCTCGATTAGGTCCAAATTCAGCCCCATTGTGGCTGCAAAATCTAGACGGGGCTTTGCTGTGAGAGCCGAGTCGGAAGGTGAAGCGGGAGCCACCGAGAATGAAGAAGCTGTTAGTGAACCGGCTTCAGAGGCCGTGGTGGAAGCTGAAGCAGAAGCAGTTCTGGAGTCTGAGCCTGAAGAAAAGAAGCCGCCGCGGAAGCCTCGTATCAAGCTCGGTGACATAATGGGGATACTAAATAGCCGGGCAATTGAGGCGGCAGAGAAGGAAAGGCCAGTTCCAGACATTAGGAGTGGAGACGTTGTGCAAATTAAACTGGAAGTTCCTGAAAACAAGCGTAGGTTGTCTATCTATAAAGGTATCGTTATTTCAAAGCAAAATGCTGGCATTCATACAACATTTCGCATTCGGAGGATAATTGCTGGCATCGGGGTTGAGATTGTTTTCCCTCTATACTCACCGAACATTAAGGAGTTGAAGGTACTTAGTCATAGGAAGGTCAGAAGGGCAAGGCTGTACTATTTGAGAGACAAACTTCCTAGGTTCTCGACTTTCAAGTGA

Protein Analysis

235

Amino Acids

26.04

Weight (kDa)

10.24

Isoelectric Point (pI)

61.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L19 PF01245 132 - 229 5.1e-31 Ribosomal protein L19
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013276)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G17560 AT5G47190
fragaria_vesca FvH4_5g20190 FvH4_5g20190
malus_domestica MD04G1051500.v1.1 MD06G1048700.v1.1
prunus_persica Prupe.5G058800_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0205151
rosa_laevigata RLG00000003395
rosa_multiflora Rmu_sc0001072.1_g000005 Rmu_sc0009349.1_g000012
rosa_roxburghii Rroxscaffold_3G00252540
rosa_rugosa Rorug07G0088500
rosa_samantha Rh7AG219400 Rh7BG215800 Rh7CG232400 Rh7DG226700
rosa_wichuraiana Rw7G018980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 343
AciI CCGC 6 cut(s) 87, 233, 338, 341, 343, 404
AcsI RAATTY 1 cut(s) 164
AcuI CTGAAG 3 cut(s) 258, 315, 345
AfaI GTAC 2 cut(s) 630, 665
AfiI CCNNNNNNNGG 2 cut(s) 22, 178
AgeI ACCGGT 1 cut(s) 64
AgsI TTSAA 4 cut(s) 112, 519, 625, 703
AjnI CCWGG 1 cut(s) 134
AluBI AGCT 5 cut(s) 73, 150, 257, 293, 361
AluI AGCT 5 cut(s) 73, 150, 257, 293, 361
Alw21I GWGCWC 1 cut(s) 152
Alw26I GTCTC 3 cut(s) 99, 438, 669
AlwNI CAGNNNCTG 1 cut(s) 28
Ama87I CYCGRG 1 cut(s) 74
AoxI GGCC 2 cut(s) 279, 419
ApeKI GCWGC 1 cut(s) 182
ApoI RAATTY 1 cut(s) 164
AsiGI ACCGGT 1 cut(s) 64
Asp700I GAANNNNTTC 1 cut(s) 306
AspA2I CCTAGG 1 cut(s) 686
AspS9I GGNCC 1 cut(s) 159
AsuC2I CCSGG 1 cut(s) 392
AsuHPI GGTGA 3 cut(s) 239, 377, 597
AvaI CYCGRG 1 cut(s) 74
AvaII GGWCC 1 cut(s) 159
AvrII CCTAGG 1 cut(s) 686
AxyI CCTNAGG 1 cut(s) 21
BanII GRGCYC 2 cut(s) 8, 152
Bbv12I GWGCWC 1 cut(s) 152
BbvI GCAGC 1 cut(s) 169
BceAI ACGGC 2 cut(s) 74, 266
BciT130I CCWGG 1 cut(s) 136
BciVI GTATCC 1 cut(s) 372
BcnI CCSGG 1 cut(s) 392
BcoDI GTCTC 3 cut(s) 99, 438, 669
BfaI CTAG 2 cut(s) 192, 687
BfuI GTATCC 1 cut(s) 372
BisI GCNGC 5 cut(s) 87, 183, 338, 341, 405
BlnI CCTAGG 1 cut(s) 686
BlsI GCNGC 5 cut(s) 88, 184, 339, 342, 406
Bme1390I CCNGG 2 cut(s) 136, 392
Bme18I GGWCC 1 cut(s) 159
BmeT110I CYCGRG 1 cut(s) 74
BmgT120I GGNCC 1 cut(s) 159
BmiI GGNNCC 2 cut(s) 7, 238
BmrFI CCNGG 2 cut(s) 136, 392
BmrI ACTGGG 1 cut(s) 129
BmsI GCATC 1 cut(s) 582
BmuI ACTGGG 1 cut(s) 129
BpmI CTGGAG 1 cut(s) 332
BpuMI CCSGG 1 cut(s) 392
BsaJI CCNNGG 4 cut(s) 135, 282, 341, 686
BsaWI WCCGGW 1 cut(s) 64
BsaXI ACNNNNNCTCC 2 cut(s) 435, 465
Bsc4I CCNNNNNNNGG 2 cut(s) 22, 178
Bse118I RCCGGY 2 cut(s) 64, 268
Bse1I ACTGG 3 cut(s) 124, 422, 468
Bse21I CCTNAGG 1 cut(s) 21
BseBI CCWGG 1 cut(s) 136
BseDI CCNNGG 4 cut(s) 135, 282, 341, 686
BseLI CCNNNNNNNGG 2 cut(s) 22, 178
BseMII CTCAG 2 cut(s) 35, 309
BseNI ACTGG 3 cut(s) 124, 422, 468
BseXI GCAGC 1 cut(s) 169
Bsh1236I CGCG 1 cut(s) 343
BshFI GGCC 2 cut(s) 281, 421
BshTI ACCGGT 1 cut(s) 64
BsiHKAI GWGCWC 1 cut(s) 152
BsiHKCI CYCGRG 1 cut(s) 74
BsiSI CCGG 3 cut(s) 65, 269, 391
BslI CCNNNNNNNGG 2 cut(s) 22, 178
BsmAI GTCTC 3 cut(s) 99, 438, 669
BsmBI CGTCTC 1 cut(s) 438
BsmI GAATGC 2 cut(s) 534, 552
BsnI GGCC 2 cut(s) 281, 421
BsoBI CYCGRG 1 cut(s) 74
Bsp1286I GDGCHC 2 cut(s) 8, 152
BspACI CCGC 6 cut(s) 87, 233, 338, 341, 343, 404
BspANI GGCC 2 cut(s) 281, 421
BspCNI CTCAG 2 cut(s) 34, 310
BspFNI CGCG 1 cut(s) 343
BspLI GGNNCC 2 cut(s) 7, 238
BsrFI RCCGGY 2 cut(s) 64, 268
BsrI ACTGG 3 cut(s) 124, 422, 468
BssAI RCCGGY 2 cut(s) 64, 268
BssECI CCNNGG 4 cut(s) 135, 282, 341, 686
BssT1I CCWWGG 1 cut(s) 686
Bst2UI CCWGG 1 cut(s) 136
BstC8I GCNNGC 2 cut(s) 532, 571
BstDEI CTNAG 3 cut(s) 21, 318, 632
BstDSI CCRYGG 2 cut(s) 282, 341
BstENI CCTNNNNNAGG 1 cut(s) 20
BstFNI CGCG 1 cut(s) 343
BstMAI GTCTC 3 cut(s) 99, 438, 669
BstMWI GCNNNNNNNGC 4 cut(s) 212, 278, 346, 401
BstNI CCWGG 1 cut(s) 136
BstSCI CCNGG 2 cut(s) 134, 390
BstUI CGCG 1 cut(s) 343
BstV1I GCAGC 1 cut(s) 169
Bsu36I CCTNAGG 1 cut(s) 21
BsuI GTATCC 1 cut(s) 372
BsuRI GGCC 2 cut(s) 281, 421
BtgI CCRYGG 2 cut(s) 282, 341
BtsIMutI CAGTG 1 cut(s) 117
Cac8I GCNNGC 2 cut(s) 532, 571
CaiI CAGNNNCTG 1 cut(s) 28
Cfr10I RCCGGY 2 cut(s) 64, 268
Cfr13I GGNCC 1 cut(s) 159
Cfr42I CCGCGG 1 cut(s) 344
Csp6I GTAC 2 cut(s) 629, 664
CspAI ACCGGT 1 cut(s) 64
CviQI GTAC 2 cut(s) 629, 664
DdeI CTNAG 3 cut(s) 21, 318, 632
Ecl136II GAGCTC 1 cut(s) 150
Eco130I CCWWGG 1 cut(s) 686
Eco24I GRGCYC 2 cut(s) 8, 152
Eco47I GGWCC 1 cut(s) 159
Eco53kI GAGCTC 1 cut(s) 150
Eco57I CTGAAG 3 cut(s) 258, 315, 345
Eco81I CCTNAGG 1 cut(s) 21
Eco88I CYCGRG 1 cut(s) 74
EcoICRI GAGCTC 1 cut(s) 150
EcoNI CCTNNNNNAGG 1 cut(s) 20
EcoRII CCWGG 1 cut(s) 134
EcoT14I CCWWGG 1 cut(s) 686
EcoT38I GRGCYC 2 cut(s) 8, 152
ErhI CCWWGG 1 cut(s) 686
Esp3I CGTCTC 1 cut(s) 438
FaiI YATR 5 cut(s) 371, 501, 540, 601, 639
FauI CCCGC 1 cut(s) 226
Fnu4HI GCNGC 5 cut(s) 87, 183, 338, 341, 405
FriOI GRGCYC 2 cut(s) 8, 152
Fsp4HI GCNGC 5 cut(s) 87, 183, 338, 341, 405
FspBI CTAG 2 cut(s) 192, 687
GluI GCNGC 5 cut(s) 87, 183, 338, 341, 405
GsuI CTGGAG 1 cut(s) 332
HaeIII GGCC 2 cut(s) 281, 421
HapII CCGG 3 cut(s) 65, 269, 391
HinfI GANTC 2 cut(s) 218, 314
HpaII CCGG 3 cut(s) 65, 269, 391
HphI GGTGA 3 cut(s) 239, 377, 597
Hpy166II GTNNAC 1 cut(s) 266
Hpy188I TCNGA 5 cut(s) 223, 277, 319, 558, 650
Hpy188III TCNNGA 5 cut(s) 192, 311, 428, 473, 694
Hpy8I GTNNAC 1 cut(s) 266
Hpy99I CGWCG 1 cut(s) 102
HpyAV CCTTC 6 cut(s) 26, 218, 406, 619, 637, 645
HpyCH4IV ACGT 1 cut(s) 447
HpyCH4V TGCA 2 cut(s) 185, 454
HpyF10VI GCNNNNNNNGC 4 cut(s) 212, 278, 346, 401
HpyF3I CTNAG 3 cut(s) 21, 318, 632
HpySE526I ACGT 1 cut(s) 447
KspI CCGCGG 1 cut(s) 344
LmnI GCTCC 2 cut(s) 11, 236
Lsp1109I GCAGC 1 cut(s) 169
LweI GCATC 1 cut(s) 582
MaeI CTAG 2 cut(s) 192, 687
MaeII ACGT 1 cut(s) 447
MaeIII GTNAC 1 cut(s) 365
MboII GAAGA 3 cut(s) 100, 263, 338
MfeI CAATTG 1 cut(s) 396
MhlI GDGCHC 2 cut(s) 8, 152
MluCI AATT 5 cut(s) 112, 164, 396, 456, 564
MlyI GAGTC 2 cut(s) 227, 323
MmeI TCCRAC 1 cut(s) 201
MnlI CCTC 6 cut(s) 30, 271, 360, 394, 552, 606
MroXI GAANNNNTTC 1 cut(s) 306
MseI TTAA 2 cut(s) 459, 615
MspA1I CMGCKG 1 cut(s) 343
MspI CCGG 3 cut(s) 65, 269, 391
MspR9I CCNGG 2 cut(s) 136, 392
MunI CAATTG 1 cut(s) 396
Mva1269I GAATGC 2 cut(s) 534, 552
MvaI CCWGG 1 cut(s) 136
MvnI CGCG 1 cut(s) 343
MwoI GCNNNNNNNGC 4 cut(s) 212, 278, 346, 401
NciI CCSGG 1 cut(s) 392
NlaIV GGNNCC 2 cut(s) 7, 238
NmeAIII GCCGAG 1 cut(s) 241
NmuCI GTSAC 1 cut(s) 365
PctI GAATGC 2 cut(s) 534, 552
PdmI GAANNNNTTC 1 cut(s) 306
PinAI ACCGGT 1 cut(s) 64
PkrI GCNGC 5 cut(s) 88, 184, 339, 342, 406
PleI GAGTC 2 cut(s) 226, 322
PpsI GAGTC 2 cut(s) 226, 322
Psp124BI GAGCTC 1 cut(s) 152
Psp6I CCWGG 1 cut(s) 134
PspGI CCWGG 1 cut(s) 134
PspN4I GGNNCC 2 cut(s) 7, 238
PspPI GGNCC 1 cut(s) 159
PstNI CAGNNNCTG 1 cut(s) 28
RsaI GTAC 2 cut(s) 630, 665
RsaNI GTAC 2 cut(s) 629, 664
SacI GAGCTC 1 cut(s) 152
SacII CCGCGG 1 cut(s) 344
SaqAI TTAA 2 cut(s) 459, 615
SatI GCNGC 5 cut(s) 87, 183, 338, 341, 405
Sau96I GGNCC 1 cut(s) 159
SchI GAGTC 2 cut(s) 227, 323
ScrFI CCNGG 2 cut(s) 136, 392
SduI GDGCHC 2 cut(s) 8, 152
SfaNI GCATC 1 cut(s) 582
Sfr303I CCGCGG 1 cut(s) 344
SgrBI CCGCGG 1 cut(s) 344
SinI GGWCC 1 cut(s) 159
Sse9I AATT 5 cut(s) 112, 164, 396, 456, 564
SsiI CCGC 6 cut(s) 87, 233, 338, 341, 343, 404
SspMI CTAG 2 cut(s) 192, 687
SstI GAGCTC 1 cut(s) 152
StyD4I CCNGG 2 cut(s) 134, 390
StyI CCWWGG 1 cut(s) 686
TaiI ACGT 1 cut(s) 450
TaqI TCGA 3 cut(s) 146, 152, 695
TaqII GACCGA 1 cut(s) 90
TasI AATT 5 cut(s) 112, 164, 396, 456, 564
TatI WGTACW 1 cut(s) 663
TauI GCSGC 4 cut(s) 89, 340, 343, 407
Tru1I TTAA 2 cut(s) 459, 615
Tru9I TTAA 2 cut(s) 459, 615
TscAI CASTG 1 cut(s) 124
TseFI GTSAC 1 cut(s) 365
TseI GCWGC 1 cut(s) 182
Tsp45I GTSAC 1 cut(s) 365
TspDTI ATGAA 3 cut(s) 132, 264, 527
TspRI CASTG 1 cut(s) 124
VpaK11BI GGWCC 1 cut(s) 159
XagI CCTNNNNNAGG 1 cut(s) 20
XapI RAATTY 1 cut(s) 164
XbaI TCTAGA 1 cut(s) 191
XmaJI CCTAGG 1 cut(s) 686
XmnI GAANNNNTTC 1 cut(s) 306
XspI CTAG 2 cut(s) 192, 687
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.