MD06G1048700.v1.1

50S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0013276
Physical Location & Seq
Reverse (-)
6569194 .. 6570593
1400 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1048700.v1.1.491

Sequence Viewer

Length: 624 bp
ATGGGCTCCAAAATTCTTCCACAGGCTCTGCTAACAATTCCAAGAAACCCTAACGAATTCCCGCCAGTAAAGCTCGGTTTTTCAGCTGCTCTCGCTCGCCGCTCTTCCAATTGCTCTCGGGTTTCGCTCTCTAGGGTTTCATCGAGCTCAATTGGGTCCGTTTTCAGTCCCATTGTTTCTGCAATGTCGAAACGGAACTTTGTTGTGAGGGCCGAGCCGAGCTCTGAAGGTGAAACCGGAGCCACGGATGATGCCCAAGAGATTGAAGCGGCTGGGAGAGGAGAGGCCTCTGTGGCCGTGGTAGAATCTGAAGCAGAAGCAGAAGCAGTGCTGGAGTCTGAACCCGAAGAGCCAAAGCCGCCGAGAAAGTCTCGGGTTAAGCTCGGAGATATAATGGGGATATTAAATAAAAGGGCAGTTGAGGCGGCCGAGCAGGAAAGGCCAGTTCCGGACATTAGGACTGGAGATGTTGTGCAAATTAAACTGGAAGTTCCTGAAAATAAGAGAAGGTTGTCTATCTATAAGGGCATTGTCATTTCTAGACAAAATGCTGGTATTCACACAACTATTCGCATTCGGAGGATTATTGCTGGCATAGGGGTTGAGATTGTGTTCCCGCTGTAA

Protein Analysis

208

Amino Acids

22.3

Weight (kDa)

9.16

Isoelectric Point (pI)

62.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L19 PF01245 139 - 207 8.8e-16 Ribosomal protein L19
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013276)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G17560 AT5G47190
fragaria_vesca FvH4_5g20190 FvH4_5g20190
malus_domestica MD04G1051500.v1.1 MD06G1048700.v1.1
prunus_persica Prupe.5G058800_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0205151
rosa_laevigata RLG00000003395
rosa_multiflora Rmu_sc0001072.1_g000005 Rmu_sc0009349.1_g000012
rosa_roxburghii Rroxscaffold_3G00252540
rosa_rugosa Rorug07G0088500
rosa_samantha Rh7AG219400 Rh7BG215800 Rh7CG232400 Rh7DG226700
rosa_wichuraiana Rw7G018980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 102
AccIII TCCGGA 1 cut(s) 448
AciI CCGC 6 cut(s) 62, 100, 269, 359, 425, 617
AcoI YGGCCR 2 cut(s) 294, 426
AcsI RAATTY 2 cut(s) 12, 56
AcuI CTGAAG 2 cut(s) 246, 330
AgsI TTSAA 1 cut(s) 266
AluBI AGCT 5 cut(s) 73, 86, 147, 222, 382
AluI AGCT 5 cut(s) 73, 86, 147, 222, 382
Alw21I GWGCWC 2 cut(s) 149, 224
Alw26I GTCTC 1 cut(s) 375
AlwNI CAGNNNCTG 1 cut(s) 28
Ama87I CYCGRG 2 cut(s) 117, 372
Aor13HI TCCGGA 1 cut(s) 448
AoxI GGCC 5 cut(s) 210, 285, 294, 426, 440
ApeKI GCWGC 1 cut(s) 86
ApoI RAATTY 2 cut(s) 12, 56
AspS9I GGNCC 2 cut(s) 156, 210
AsuHPI GGTGA 1 cut(s) 242
AvaI CYCGRG 2 cut(s) 117, 372
AvaII GGWCC 1 cut(s) 156
BanII GRGCYC 3 cut(s) 8, 149, 224
Bbv12I GWGCWC 2 cut(s) 149, 224
BbvI GCAGC 1 cut(s) 73
BceAI ACGGC 1 cut(s) 281
BcoDI GTCTC 1 cut(s) 375
BfaI CTAG 2 cut(s) 132, 540
BglI GCCNNNNNGGC 1 cut(s) 293
BisI GCNGC 5 cut(s) 87, 100, 270, 359, 426
BlsI GCNGC 5 cut(s) 88, 101, 271, 360, 427
Bme18I GGWCC 1 cut(s) 156
BmeT110I CYCGRG 2 cut(s) 117, 372
BmgT120I GGNCC 2 cut(s) 156, 210
BmiI GGNNCC 3 cut(s) 7, 157, 241
BmsI GCATC 1 cut(s) 241
BplI GAGNNNNNCTC 4 cut(s) 206, 238, 355, 387
BpmI CTGGAG 2 cut(s) 353, 483
BsaJI CCNNGG 2 cut(s) 243, 297
BsaWI WCCGGW 2 cut(s) 236, 448
BsaXI ACNNNNNCTCC 2 cut(s) 456, 486
Bse1I ACTGG 4 cut(s) 65, 443, 466, 489
Bse3DI GCAATG 1 cut(s) 189
BseAI TCCGGA 1 cut(s) 448
BseDI CCNNGG 2 cut(s) 243, 297
BseGI GGATG 1 cut(s) 253
BseMI GCAATG 1 cut(s) 189
BseNI ACTGG 4 cut(s) 65, 443, 466, 489
BseRI GAGGAG 1 cut(s) 294
BseX3I CGGCCG 1 cut(s) 426
BseXI GCAGC 1 cut(s) 73
BseYI CCCAGC 1 cut(s) 272
Bsh1285I CGRYCG 1 cut(s) 429
BshFI GGCC 5 cut(s) 212, 287, 296, 428, 442
BsiEI CGRYCG 1 cut(s) 429
BsiHKAI GWGCWC 2 cut(s) 149, 224
BsiHKCI CYCGRG 2 cut(s) 117, 372
BsiSI CCGG 2 cut(s) 237, 449
BslFI GGGAC 1 cut(s) 153
BsmAI GTCTC 1 cut(s) 375
BsmFI GGGAC 1 cut(s) 153
BsmI GAATGC 1 cut(s) 573
BsnI GGCC 5 cut(s) 212, 287, 296, 428, 442
BsoBI CYCGRG 2 cut(s) 117, 372
Bsp1286I GDGCHC 3 cut(s) 8, 149, 224
Bsp13I TCCGGA 1 cut(s) 448
BspACI CCGC 6 cut(s) 62, 100, 269, 359, 425, 617
BspANI GGCC 5 cut(s) 212, 287, 296, 428, 442
BspEI TCCGGA 1 cut(s) 448
BspLI GGNNCC 3 cut(s) 7, 157, 241
BspQI GCTCTTC 2 cut(s) 109, 342
BsrBI CCGCTC 1 cut(s) 102
BsrDI GCAATG 1 cut(s) 189
BsrI ACTGG 4 cut(s) 65, 443, 466, 489
BssECI CCNNGG 2 cut(s) 243, 297
Bst6I CTCTTC 2 cut(s) 109, 342
BstC8I GCNNGC 2 cut(s) 97, 592
BstDSI CCRYGG 2 cut(s) 243, 297
BstF5I GGATG 1 cut(s) 253
BstMAI GTCTC 1 cut(s) 375
BstMCI CGRYCG 1 cut(s) 429
BstMWI GCNNNNNNNGC 6 cut(s) 70, 92, 293, 358, 422, 439
BstV1I GCAGC 1 cut(s) 73
BstZI CGGCCG 1 cut(s) 426
BsuRI GGCC 5 cut(s) 212, 287, 296, 428, 442
BtgI CCRYGG 2 cut(s) 243, 297
BtsCI GGATG 1 cut(s) 253
BtsI GCAGTG 1 cut(s) 333
BtsIMutI CAGTG 1 cut(s) 333
Cac8I GCNNGC 2 cut(s) 97, 592
CaiI CAGNNNCTG 1 cut(s) 28
Cfr13I GGNCC 2 cut(s) 156, 210
EaeI YGGCCR 2 cut(s) 294, 426
EagI CGGCCG 1 cut(s) 426
Eam1104I CTCTTC 2 cut(s) 109, 342
EarI CTCTTC 2 cut(s) 109, 342
Ecl136II GAGCTC 2 cut(s) 147, 222
EclXI CGGCCG 1 cut(s) 426
Eco147I AGGCCT 1 cut(s) 287
Eco24I GRGCYC 3 cut(s) 8, 149, 224
Eco47I GGWCC 1 cut(s) 156
Eco52I CGGCCG 1 cut(s) 426
Eco53kI GAGCTC 2 cut(s) 147, 222
Eco57I CTGAAG 2 cut(s) 246, 330
Eco88I CYCGRG 2 cut(s) 117, 372
EcoICRI GAGCTC 2 cut(s) 147, 222
EcoRI GAATTC 1 cut(s) 56
EcoT38I GRGCYC 3 cut(s) 8, 149, 224
FaiI YATR 3 cut(s) 392, 522, 596
FaqI GGGAC 1 cut(s) 153
FauI CCCGC 1 cut(s) 69
Fnu4HI GCNGC 5 cut(s) 87, 100, 270, 359, 426
FokI GGATG 1 cut(s) 260
FriOI GRGCYC 3 cut(s) 8, 149, 224
Fsp4HI GCNGC 5 cut(s) 87, 100, 270, 359, 426
FspBI CTAG 2 cut(s) 132, 540
GluI GCNGC 5 cut(s) 87, 100, 270, 359, 426
GsaI CCCAGC 1 cut(s) 276
GsuI CTGGAG 2 cut(s) 353, 483
HaeIII GGCC 5 cut(s) 212, 287, 296, 428, 442
HapII CCGG 2 cut(s) 237, 449
HinfI GANTC 2 cut(s) 305, 335
HpaII CCGG 2 cut(s) 237, 449
HphI GGTGA 1 cut(s) 242
Hpy188I TCNGA 5 cut(s) 226, 310, 340, 386, 579
Hpy188III TCNNGA 3 cut(s) 449, 494, 540
HpyAV CCTTC 2 cut(s) 221, 501
HpyCH4V TGCA 2 cut(s) 182, 475
HpyF10VI GCNNNNNNNGC 6 cut(s) 70, 92, 293, 358, 422, 439
Kpn2I TCCGGA 1 cut(s) 448
LguI GCTCTTC 2 cut(s) 109, 342
LmnI GCTCC 2 cut(s) 11, 239
Lsp1109I GCAGC 1 cut(s) 73
LweI GCATC 1 cut(s) 241
MaeI CTAG 2 cut(s) 132, 540
MbiI CCGCTC 1 cut(s) 102
MboII GAAGA 3 cut(s) 8, 96, 359
MfeI CAATTG 2 cut(s) 109, 150
MhlI GDGCHC 3 cut(s) 8, 149, 224
MluCI AATT 6 cut(s) 12, 36, 56, 109, 150, 477
MlyI GAGTC 1 cut(s) 344
MnlI CCTC 6 cut(s) 201, 272, 277, 298, 415, 573
MroI TCCGGA 1 cut(s) 448
MseI TTAA 3 cut(s) 378, 404, 480
MspA1I CMGCKG 2 cut(s) 86, 619
MspI CCGG 2 cut(s) 237, 449
MunI CAATTG 2 cut(s) 109, 150
Mva1269I GAATGC 1 cut(s) 573
MwoI GCNNNNNNNGC 6 cut(s) 70, 92, 293, 358, 422, 439
NlaIV GGNNCC 3 cut(s) 7, 157, 241
NmeAIII GCCGAG 4 cut(s) 238, 243, 387, 454
PceI AGGCCT 1 cut(s) 287
PciSI GCTCTTC 2 cut(s) 109, 342
PctI GAATGC 1 cut(s) 573
PfeI GAWTC 1 cut(s) 305
PkrI GCNGC 5 cut(s) 88, 101, 271, 360, 427
PleI GAGTC 1 cut(s) 343
PpsI GAGTC 1 cut(s) 343
Psp124BI GAGCTC 2 cut(s) 149, 224
PspFI CCCAGC 1 cut(s) 272
PspN4I GGNNCC 3 cut(s) 7, 157, 241
PspPI GGNCC 2 cut(s) 156, 210
PstNI CAGNNNCTG 1 cut(s) 28
PvuII CAGCTG 1 cut(s) 86
SacI GAGCTC 2 cut(s) 149, 224
SapI GCTCTTC 2 cut(s) 109, 342
SaqAI TTAA 3 cut(s) 378, 404, 480
SatI GCNGC 5 cut(s) 87, 100, 270, 359, 426
Sau96I GGNCC 2 cut(s) 156, 210
SchI GAGTC 1 cut(s) 344
SduI GDGCHC 3 cut(s) 8, 149, 224
SetI ASST 7 cut(s) 75, 88, 149, 224, 232, 384, 512
SfaNI GCATC 1 cut(s) 241
SfiI GGCCNNNNNGGCC 1 cut(s) 293
SinI GGWCC 1 cut(s) 156
Sse9I AATT 6 cut(s) 12, 36, 56, 109, 150, 477
SseBI AGGCCT 1 cut(s) 287
SsiI CCGC 6 cut(s) 62, 100, 269, 359, 425, 617
SspMI CTAG 2 cut(s) 132, 540
SstI GAGCTC 2 cut(s) 149, 224
StuI AGGCCT 1 cut(s) 287
TaqI TCGA 2 cut(s) 143, 188
TasI AATT 6 cut(s) 12, 36, 56, 109, 150, 477
TauI GCSGC 4 cut(s) 102, 272, 361, 428
TfiI GAWTC 1 cut(s) 305
Tru1I TTAA 3 cut(s) 378, 404, 480
Tru9I TTAA 3 cut(s) 378, 404, 480
TscAI CASTG 1 cut(s) 333
TseI GCWGC 1 cut(s) 86
TspDTI ATGAA 1 cut(s) 129
TspGWI ACGGA 3 cut(s) 148, 208, 260
TspRI CASTG 1 cut(s) 333
VpaK11BI GGWCC 1 cut(s) 156
XapI RAATTY 2 cut(s) 12, 56
XbaI TCTAGA 1 cut(s) 539
XspI CTAG 2 cut(s) 132, 540
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.