MD04G1051500.v1.1

50S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0013276
Physical Location & Seq
Forward (+)
6049738 .. 6051996
2259 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1051500.v1.1.491

Sequence Viewer

Length: 726 bp
ATGGGCTCCAAAATTCTTCCACAGGCTCTGATAACAATTCCACGAAACCCTAATCAATACCCACCAGTAAAGCTCGGGTTTTCAGCTGCCCTCTCTCGCCGCTCTTCCAATTCCGCCCGGGTTTCGCTCTCTAGGGTTTCATCGAGCTCAATTGGGTCCGTTTTCAGTCCCATTGTTTCCGCGATATCGAAACGGAACTTCGTTGTGAGAGCAGGCCCGAGCTCTGAAGGTGAAGTCGGAGCCACTGATGATGCCCAAGAGAATGAAGCAACTGGGAGCGGAGAGGCCTCTGTGGCCGTTGTAGAAGCGGAAGCAGAAGCAGTGCTGGAGCCTGAATCCGAAGAGCCGAAGCCGCCAAGAAAGTCTCGGGTTAAGCTCGGAGATATAATGGGGGTAATATTAAACAAAAGGGCAGTTGAGGCGGCAGAGCAGGAAAGGCCAGTTCCCGACATTAGAACTGGAGATGTTGTGCAAATTAAACTGGAAGTTCCTGAAAATAAGAGAAGACTGTCTATCTATAAAGGCATTGTCATTTCAAGACAAAATGCTGGTATTCACACAACTATTCGCATTCGGAGGATTATTGCTGGCGTAGGGGTTGAGATTGTGTTCCCGCTATACTCACCAAACATTAAGGAGCTAAAGGTACTGAGTCACAGGAAGGTCAGGAGGGCAAGGTTGTACTATTTGAGAGACAAGCTTCCAAGGCTTTCGACGTTCAAATGA

Protein Analysis

242

Amino Acids

26.37

Weight (kDa)

10.23

Isoelectric Point (pI)

53.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L19 PF01245 138 - 236 1e-30 Ribosomal protein L19
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013276)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G17560 AT5G47190
fragaria_vesca FvH4_5g20190 FvH4_5g20190
malus_domestica MD04G1051500.v1.1 MD06G1048700.v1.1
prunus_persica Prupe.5G058800_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0205151
rosa_laevigata RLG00000003395
rosa_multiflora Rmu_sc0001072.1_g000005 Rmu_sc0009349.1_g000012
rosa_roxburghii Rroxscaffold_3G00252540
rosa_rugosa Rorug07G0088500
rosa_samantha Rh7AG219400 Rh7BG215800 Rh7CG232400 Rh7DG226700
rosa_wichuraiana Rw7G018980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 102, 279
AccII CGCG 1 cut(s) 182
AciI CCGC 8 cut(s) 100, 114, 180, 279, 308, 353, 422, 614
AcoI YGGCCR 1 cut(s) 294
AcsI RAATTY 1 cut(s) 12
AcuI CTGAAG 1 cut(s) 246
AfaI GTAC 2 cut(s) 648, 683
AgsI TTSAA 2 cut(s) 537, 721
AluBI AGCT 7 cut(s) 73, 86, 147, 222, 376, 640, 700
AluI AGCT 7 cut(s) 73, 86, 147, 222, 376, 640, 700
Alw21I GWGCWC 2 cut(s) 149, 224
Alw26I GTCTC 2 cut(s) 369, 687
AlwNI CAGNNNCTG 1 cut(s) 28
Ama87I CYCGRG 4 cut(s) 74, 117, 217, 366
AoxI GGCC 4 cut(s) 214, 285, 294, 437
ApeKI GCWGC 1 cut(s) 86
ApoI RAATTY 1 cut(s) 12
AspS9I GGNCC 2 cut(s) 156, 215
AsuC2I CCSGG 2 cut(s) 118, 119
AsuHPI GGTGA 2 cut(s) 242, 615
AvaI CYCGRG 4 cut(s) 74, 117, 217, 366
AvaII GGWCC 1 cut(s) 156
BanII GRGCYC 3 cut(s) 8, 149, 224
BbsI GAAGAC 1 cut(s) 511
Bbv12I GWGCWC 2 cut(s) 149, 224
BbvI GCAGC 1 cut(s) 73
BceAI ACGGC 1 cut(s) 281
BcnI CCSGG 2 cut(s) 118, 119
BcoDI GTCTC 2 cut(s) 369, 687
BfaI CTAG 1 cut(s) 132
BglI GCCNNNNNGGC 1 cut(s) 293
BisI GCNGC 4 cut(s) 87, 100, 353, 423
BlsI GCNGC 4 cut(s) 88, 101, 354, 424
Bme1390I CCNGG 2 cut(s) 118, 119
Bme18I GGWCC 1 cut(s) 156
BmeT110I CYCGRG 4 cut(s) 74, 117, 217, 366
BmgT120I GGNCC 2 cut(s) 156, 215
BmiI GGNNCC 4 cut(s) 7, 157, 241, 330
BmrFI CCNGG 2 cut(s) 118, 119
BmrI ACTGGG 1 cut(s) 282
BmsI GCATC 1 cut(s) 241
BmuI ACTGGG 1 cut(s) 282
BpiI GAAGAC 1 cut(s) 511
BpmI CTGGAG 2 cut(s) 347, 480
BpuMI CCSGG 2 cut(s) 118, 119
BsaJI CCNNGG 2 cut(s) 117, 704
BsaXI ACNNNNNCTCC 2 cut(s) 453, 483
Bse1I ACTGG 5 cut(s) 65, 277, 440, 463, 486
BseDI CCNNGG 2 cut(s) 117, 704
BseMII CTCAG 1 cut(s) 641
BseNI ACTGG 5 cut(s) 65, 277, 440, 463, 486
BseXI GCAGC 1 cut(s) 73
Bsh1236I CGCG 1 cut(s) 182
BshFI GGCC 4 cut(s) 216, 287, 296, 439
BsiHKAI GWGCWC 2 cut(s) 149, 224
BsiHKCI CYCGRG 4 cut(s) 74, 117, 217, 366
BsiSI CCGG 1 cut(s) 118
BslFI GGGAC 1 cut(s) 153
BsmAI GTCTC 2 cut(s) 369, 687
BsmFI GGGAC 1 cut(s) 153
BsmI GAATGC 1 cut(s) 570
BsnI GGCC 4 cut(s) 216, 287, 296, 439
BsoBI CYCGRG 4 cut(s) 74, 117, 217, 366
Bsp1286I GDGCHC 3 cut(s) 8, 149, 224
BspACI CCGC 8 cut(s) 100, 114, 180, 279, 308, 353, 422, 614
BspANI GGCC 4 cut(s) 216, 287, 296, 439
BspCNI CTCAG 1 cut(s) 642
BspFNI CGCG 1 cut(s) 182
BspLI GGNNCC 4 cut(s) 7, 157, 241, 330
BspQI GCTCTTC 2 cut(s) 109, 336
BsrBI CCGCTC 2 cut(s) 102, 279
BsrI ACTGG 5 cut(s) 65, 277, 440, 463, 486
BssECI CCNNGG 2 cut(s) 117, 704
BssT1I CCWWGG 1 cut(s) 704
Bst4CI ACNGT 1 cut(s) 510
Bst6I CTCTTC 2 cut(s) 109, 336
BstC8I GCNNGC 2 cut(s) 214, 589
BstDEI CTNAG 1 cut(s) 650
BstFNI CGCG 1 cut(s) 182
BstMAI GTCTC 2 cut(s) 369, 687
BstMWI GCNNNNNNNGC 5 cut(s) 293, 352, 419, 436, 706
BstSCI CCNGG 2 cut(s) 116, 117
BstUI CGCG 1 cut(s) 182
BstV1I GCAGC 1 cut(s) 73
BstV2I GAAGAC 1 cut(s) 511
BsuRI GGCC 4 cut(s) 216, 287, 296, 439
BtsI GCAGTG 1 cut(s) 327
BtsIMutI CAGTG 2 cut(s) 243, 327
Cac8I GCNNGC 2 cut(s) 214, 589
CaiI CAGNNNCTG 1 cut(s) 28
Cfr13I GGNCC 2 cut(s) 156, 215
Cfr9I CCCGGG 1 cut(s) 117
Csp6I GTAC 2 cut(s) 647, 682
CviQI GTAC 2 cut(s) 647, 682
DdeI CTNAG 1 cut(s) 650
EaeI YGGCCR 1 cut(s) 294
Eam1104I CTCTTC 2 cut(s) 109, 336
EarI CTCTTC 2 cut(s) 109, 336
EciI GGCGGA 1 cut(s) 103
Ecl136II GAGCTC 2 cut(s) 147, 222
Eco130I CCWWGG 1 cut(s) 704
Eco147I AGGCCT 1 cut(s) 287
Eco24I GRGCYC 3 cut(s) 8, 149, 224
Eco32I GATATC 1 cut(s) 186
Eco47I GGWCC 1 cut(s) 156
Eco53kI GAGCTC 2 cut(s) 147, 222
Eco57I CTGAAG 1 cut(s) 246
Eco88I CYCGRG 4 cut(s) 74, 117, 217, 366
EcoICRI GAGCTC 2 cut(s) 147, 222
EcoRV GATATC 1 cut(s) 186
EcoT14I CCWWGG 1 cut(s) 704
EcoT38I GRGCYC 3 cut(s) 8, 149, 224
ErhI CCWWGG 1 cut(s) 704
FaiI YATR 3 cut(s) 386, 519, 619
FaqI GGGAC 1 cut(s) 153
FauI CCCGC 1 cut(s) 621
Fnu4HI GCNGC 4 cut(s) 87, 100, 353, 423
FriOI GRGCYC 3 cut(s) 8, 149, 224
Fsp4HI GCNGC 4 cut(s) 87, 100, 353, 423
FspBI CTAG 1 cut(s) 132
GluI GCNGC 4 cut(s) 87, 100, 353, 423
GsuI CTGGAG 2 cut(s) 347, 480
HaeIII GGCC 4 cut(s) 216, 287, 296, 439
HapII CCGG 1 cut(s) 118
HindIII AAGCTT 1 cut(s) 698
HinfI GANTC 2 cut(s) 335, 652
HpaII CCGG 1 cut(s) 118
HphI GGTGA 2 cut(s) 242, 615
Hpy188I TCNGA 6 cut(s) 30, 226, 239, 340, 380, 576
Hpy188III TCNNGA 4 cut(s) 446, 491, 537, 667
Hpy99I CGWCG 1 cut(s) 718
HpyAV CCTTC 2 cut(s) 221, 655
HpyCH4III ACNGT 1 cut(s) 510
HpyCH4IV ACGT 1 cut(s) 716
HpyCH4V TGCA 1 cut(s) 472
HpyF10VI GCNNNNNNNGC 5 cut(s) 293, 352, 419, 436, 706
HpyF3I CTNAG 1 cut(s) 650
HpySE526I ACGT 1 cut(s) 716
LguI GCTCTTC 2 cut(s) 109, 336
LmnI GCTCC 5 cut(s) 11, 239, 276, 328, 637
Lsp1109I GCAGC 1 cut(s) 73
LweI GCATC 1 cut(s) 241
MaeI CTAG 1 cut(s) 132
MaeII ACGT 1 cut(s) 716
MaeIII GTNAC 1 cut(s) 653
MbiI CCGCTC 2 cut(s) 102, 279
MboII GAAGA 4 cut(s) 8, 96, 353, 516
MfeI CAATTG 1 cut(s) 150
MhlI GDGCHC 3 cut(s) 8, 149, 224
MluCI AATT 5 cut(s) 12, 36, 109, 150, 474
MlyI GAGTC 1 cut(s) 661
MmeI TCCRAC 1 cut(s) 217
MnlI CCTC 6 cut(s) 101, 277, 298, 412, 570, 663
MseI TTAA 4 cut(s) 372, 401, 477, 633
MspA1I CMGCKG 1 cut(s) 86
MspI CCGG 1 cut(s) 118
MspR9I CCNGG 2 cut(s) 118, 119
MunI CAATTG 1 cut(s) 150
Mva1269I GAATGC 1 cut(s) 570
MvnI CGCG 1 cut(s) 182
MwoI GCNNNNNNNGC 5 cut(s) 293, 352, 419, 436, 706
NciI CCSGG 2 cut(s) 118, 119
NlaIV GGNNCC 4 cut(s) 7, 157, 241, 330
NmuCI GTSAC 1 cut(s) 653
PceI AGGCCT 1 cut(s) 287
PciSI GCTCTTC 2 cut(s) 109, 336
PctI GAATGC 1 cut(s) 570
PfeI GAWTC 1 cut(s) 335
PkrI GCNGC 4 cut(s) 88, 101, 354, 424
PleI GAGTC 1 cut(s) 660
PpsI GAGTC 1 cut(s) 660
Psp124BI GAGCTC 2 cut(s) 149, 224
PspN4I GGNNCC 4 cut(s) 7, 157, 241, 330
PspPI GGNCC 2 cut(s) 156, 215
PstNI CAGNNNCTG 1 cut(s) 28
PvuII CAGCTG 1 cut(s) 86
RsaI GTAC 2 cut(s) 648, 683
RsaNI GTAC 2 cut(s) 647, 682
SacI GAGCTC 2 cut(s) 149, 224
SapI GCTCTTC 2 cut(s) 109, 336
SaqAI TTAA 4 cut(s) 372, 401, 477, 633
SatI GCNGC 4 cut(s) 87, 100, 353, 423
Sau96I GGNCC 2 cut(s) 156, 215
SchI GAGTC 1 cut(s) 661
ScrFI CCNGG 2 cut(s) 118, 119
SduI GDGCHC 3 cut(s) 8, 149, 224
SfaNI GCATC 1 cut(s) 241
SfiI GGCCNNNNNGGCC 1 cut(s) 293
SinI GGWCC 1 cut(s) 156
SmaI CCCGGG 1 cut(s) 119
Sse9I AATT 5 cut(s) 12, 36, 109, 150, 474
SseBI AGGCCT 1 cut(s) 287
SsiI CCGC 8 cut(s) 100, 114, 180, 279, 308, 353, 422, 614
SspI AATATT 1 cut(s) 399
SspMI CTAG 1 cut(s) 132
SstI GAGCTC 2 cut(s) 149, 224
StuI AGGCCT 1 cut(s) 287
StyD4I CCNGG 2 cut(s) 116, 117
StyI CCWWGG 1 cut(s) 704
TaaI ACNGT 1 cut(s) 510
TaiI ACGT 1 cut(s) 719
TaqI TCGA 3 cut(s) 143, 188, 713
TasI AATT 5 cut(s) 12, 36, 109, 150, 474
TatI WGTACW 1 cut(s) 681
TauI GCSGC 3 cut(s) 102, 355, 425
TfiI GAWTC 1 cut(s) 335
Tru1I TTAA 4 cut(s) 372, 401, 477, 633
Tru9I TTAA 4 cut(s) 372, 401, 477, 633
TscAI CASTG 2 cut(s) 250, 327
TseFI GTSAC 1 cut(s) 653
TseI GCWGC 1 cut(s) 86
Tsp45I GTSAC 1 cut(s) 653
TspDTI ATGAA 2 cut(s) 129, 279
TspGWI ACGGA 2 cut(s) 148, 208
TspMI CCCGGG 1 cut(s) 117
TspRI CASTG 2 cut(s) 250, 327
VpaK11BI GGWCC 1 cut(s) 156
XapI RAATTY 1 cut(s) 12
XmaI CCCGGG 1 cut(s) 117
XspI CTAG 1 cut(s) 132
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.