Rroxscaffold_3G00252540

50S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0013276
Physical Location & Seq
Reverse (-)
46566653 .. 46570416
3764 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00252540.1

Sequence Viewer

Length: 714 bp
ATGGGCTCCAAAGTCCTTCCTCAGGCTCTGCTAACAATCCCAAGAAACCCTAACCACTACGCACCGGTAAAGCTCGGGTTTTCAGCCGCCGTGTCTCGTCGGTCTTTCTTCAATTCCACTGGGGTTTCGCTTTCCAGGGCTTCATCGAGCTCGATTAGGTCCAAATTCAGCCCCATTGTGGCCGCAAAATCTAGACGGGGCTTTGCTGTGAGAGCCGAGTCGGAAGGTGAAGCGGGAGCCACCGAGAATGAAGAAGCTGTTAGTGAACCGGCTTCAGAGGCCGTGGTGGAAGCTGAAGCTGAAGCAGAAGCAGTTCTGGAGTCTGAGCCTGAAGAAAAGAAGCCGCCGCGGAAGCCTCGTATCAAGCTCGGTGACATAATGGGGATACTAAATAGCCGGGCAATTGAGGCGGCAGAGAAGGAAAGGCCAGTTCCAGACATTAGGAGTGGAGACGTTGTGCAAATTAAACTGGAGGTTCCTGAAAACAAGCGTAGGTTGTCTATCTATAAAGGTATCGTTATTTCAAAGCAAAATGCTGGCATTCATACAACATTTCGCATTCGGAGGATTATTGCTGGCATAGGGGTTGAGATTGTTTTCCCTCTATACTCACCGAACATTAAGGAGTTGAAGGTACTTAGTCATAGGAAGGTCAGAAGGGCAAGGCTGTACTATTTGAGAGACAAACTTCCTAGGTTCTCGACTTTCAAGTGA

Protein Analysis

237

Amino Acids

26.24

Weight (kDa)

10.16

Isoelectric Point (pI)

61.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L19 PF01245 134 - 231 5.2e-31 Ribosomal protein L19
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013276)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G17560 AT5G47190
fragaria_vesca FvH4_5g20190 FvH4_5g20190
malus_domestica MD04G1051500.v1.1 MD06G1048700.v1.1
prunus_persica Prupe.5G058800_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0205151
rosa_laevigata RLG00000003395
rosa_multiflora Rmu_sc0001072.1_g000005 Rmu_sc0009349.1_g000012
rosa_roxburghii Rroxscaffold_3G00252540
rosa_rugosa Rorug07G0088500
rosa_samantha Rh7AG219400 Rh7BG215800 Rh7CG232400 Rh7DG226700
rosa_wichuraiana Rw7G018980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 349
AciI CCGC 7 cut(s) 87, 183, 233, 344, 347, 349, 410
AcoI YGGCCR 1 cut(s) 180
AcsI RAATTY 1 cut(s) 164
AcuI CTGAAG 4 cut(s) 258, 315, 321, 351
AfaI GTAC 2 cut(s) 636, 671
AfiI CCNNNNNNNGG 2 cut(s) 22, 178
AgeI ACCGGT 1 cut(s) 64
AgsI TTSAA 4 cut(s) 112, 525, 631, 709
AjnI CCWGG 1 cut(s) 134
AluBI AGCT 6 cut(s) 73, 150, 257, 293, 299, 367
AluI AGCT 6 cut(s) 73, 150, 257, 293, 299, 367
Alw21I GWGCWC 1 cut(s) 152
Alw26I GTCTC 3 cut(s) 99, 444, 675
AlwNI CAGNNNCTG 1 cut(s) 28
Ama87I CYCGRG 1 cut(s) 74
AoxI GGCC 3 cut(s) 180, 279, 425
ApoI RAATTY 1 cut(s) 164
AsiGI ACCGGT 1 cut(s) 64
Asp700I GAANNNNTTC 1 cut(s) 312
AspA2I CCTAGG 1 cut(s) 692
AspS9I GGNCC 1 cut(s) 159
AsuC2I CCSGG 1 cut(s) 398
AsuHPI GGTGA 3 cut(s) 239, 383, 603
AvaI CYCGRG 1 cut(s) 74
AvaII GGWCC 1 cut(s) 159
AvrII CCTAGG 1 cut(s) 692
AxyI CCTNAGG 1 cut(s) 21
BanII GRGCYC 2 cut(s) 8, 152
Bbv12I GWGCWC 1 cut(s) 152
BceAI ACGGC 2 cut(s) 74, 266
BciT130I CCWGG 1 cut(s) 136
BciVI GTATCC 1 cut(s) 378
BcnI CCSGG 1 cut(s) 398
BcoDI GTCTC 3 cut(s) 99, 444, 675
BfaI CTAG 2 cut(s) 192, 693
BfuI GTATCC 1 cut(s) 378
BisI GCNGC 5 cut(s) 87, 183, 344, 347, 411
BlnI CCTAGG 1 cut(s) 692
BlsI GCNGC 5 cut(s) 88, 184, 345, 348, 412
Bme1390I CCNGG 2 cut(s) 136, 398
Bme18I GGWCC 1 cut(s) 159
BmeT110I CYCGRG 1 cut(s) 74
BmgT120I GGNCC 1 cut(s) 159
BmiI GGNNCC 3 cut(s) 7, 238, 477
BmrFI CCNGG 2 cut(s) 136, 398
BmrI ACTGGG 1 cut(s) 129
BmuI ACTGGG 1 cut(s) 129
BpmI CTGGAG 2 cut(s) 338, 491
BpuMI CCSGG 1 cut(s) 398
BsaJI CCNNGG 4 cut(s) 135, 282, 347, 692
BsaWI WCCGGW 1 cut(s) 64
BsaXI ACNNNNNCTCC 2 cut(s) 441, 471
Bsc4I CCNNNNNNNGG 2 cut(s) 22, 178
Bse118I RCCGGY 2 cut(s) 64, 268
Bse1I ACTGG 3 cut(s) 124, 428, 474
Bse21I CCTNAGG 1 cut(s) 21
BseBI CCWGG 1 cut(s) 136
BseDI CCNNGG 4 cut(s) 135, 282, 347, 692
BseLI CCNNNNNNNGG 2 cut(s) 22, 178
BseMII CTCAG 2 cut(s) 35, 315
BseNI ACTGG 3 cut(s) 124, 428, 474
Bsh1236I CGCG 1 cut(s) 349
BshFI GGCC 3 cut(s) 182, 281, 427
BshTI ACCGGT 1 cut(s) 64
BsiHKAI GWGCWC 1 cut(s) 152
BsiHKCI CYCGRG 1 cut(s) 74
BsiSI CCGG 3 cut(s) 65, 269, 397
BslI CCNNNNNNNGG 2 cut(s) 22, 178
BsmAI GTCTC 3 cut(s) 99, 444, 675
BsmBI CGTCTC 1 cut(s) 444
BsmI GAATGC 2 cut(s) 540, 558
BsnI GGCC 3 cut(s) 182, 281, 427
BsoBI CYCGRG 1 cut(s) 74
Bsp1286I GDGCHC 2 cut(s) 8, 152
BspACI CCGC 7 cut(s) 87, 183, 233, 344, 347, 349, 410
BspANI GGCC 3 cut(s) 182, 281, 427
BspCNI CTCAG 2 cut(s) 34, 316
BspFNI CGCG 1 cut(s) 349
BspLI GGNNCC 3 cut(s) 7, 238, 477
BsrFI RCCGGY 2 cut(s) 64, 268
BsrI ACTGG 3 cut(s) 124, 428, 474
BssAI RCCGGY 2 cut(s) 64, 268
BssECI CCNNGG 4 cut(s) 135, 282, 347, 692
BssT1I CCWWGG 1 cut(s) 692
Bst2UI CCWGG 1 cut(s) 136
BstC8I GCNNGC 2 cut(s) 538, 577
BstDEI CTNAG 3 cut(s) 21, 324, 638
BstDSI CCRYGG 2 cut(s) 282, 347
BstENI CCTNNNNNAGG 1 cut(s) 20
BstFNI CGCG 1 cut(s) 349
BstMAI GTCTC 3 cut(s) 99, 444, 675
BstMWI GCNNNNNNNGC 4 cut(s) 212, 278, 352, 407
BstNI CCWGG 1 cut(s) 136
BstSCI CCNGG 2 cut(s) 134, 396
BstUI CGCG 1 cut(s) 349
Bsu36I CCTNAGG 1 cut(s) 21
BsuI GTATCC 1 cut(s) 378
BsuRI GGCC 3 cut(s) 182, 281, 427
BtgI CCRYGG 2 cut(s) 282, 347
BtsIMutI CAGTG 1 cut(s) 117
Cac8I GCNNGC 2 cut(s) 538, 577
CaiI CAGNNNCTG 1 cut(s) 28
Cfr10I RCCGGY 2 cut(s) 64, 268
Cfr13I GGNCC 1 cut(s) 159
Cfr42I CCGCGG 1 cut(s) 350
Csp6I GTAC 2 cut(s) 635, 670
CspAI ACCGGT 1 cut(s) 64
CviQI GTAC 2 cut(s) 635, 670
DdeI CTNAG 3 cut(s) 21, 324, 638
EaeI YGGCCR 1 cut(s) 180
Ecl136II GAGCTC 1 cut(s) 150
Eco130I CCWWGG 1 cut(s) 692
Eco24I GRGCYC 2 cut(s) 8, 152
Eco47I GGWCC 1 cut(s) 159
Eco53kI GAGCTC 1 cut(s) 150
Eco57I CTGAAG 4 cut(s) 258, 315, 321, 351
Eco81I CCTNAGG 1 cut(s) 21
Eco88I CYCGRG 1 cut(s) 74
EcoICRI GAGCTC 1 cut(s) 150
EcoNI CCTNNNNNAGG 1 cut(s) 20
EcoRII CCWGG 1 cut(s) 134
EcoT14I CCWWGG 1 cut(s) 692
EcoT38I GRGCYC 2 cut(s) 8, 152
ErhI CCWWGG 1 cut(s) 692
Esp3I CGTCTC 1 cut(s) 444
FaiI YATR 6 cut(s) 377, 507, 546, 581, 607, 645
FauI CCCGC 1 cut(s) 226
Fnu4HI GCNGC 5 cut(s) 87, 183, 344, 347, 411
FriOI GRGCYC 2 cut(s) 8, 152
Fsp4HI GCNGC 5 cut(s) 87, 183, 344, 347, 411
FspBI CTAG 2 cut(s) 192, 693
GluI GCNGC 5 cut(s) 87, 183, 344, 347, 411
GsuI CTGGAG 2 cut(s) 338, 491
HaeIII GGCC 3 cut(s) 182, 281, 427
HapII CCGG 3 cut(s) 65, 269, 397
HinfI GANTC 2 cut(s) 218, 320
HpaII CCGG 3 cut(s) 65, 269, 397
HphI GGTGA 3 cut(s) 239, 383, 603
Hpy166II GTNNAC 1 cut(s) 266
Hpy188I TCNGA 5 cut(s) 223, 277, 325, 564, 656
Hpy188III TCNNGA 5 cut(s) 192, 317, 434, 479, 700
Hpy8I GTNNAC 1 cut(s) 266
Hpy99I CGWCG 1 cut(s) 102
HpyAV CCTTC 6 cut(s) 26, 218, 412, 625, 643, 651
HpyCH4IV ACGT 1 cut(s) 453
HpyCH4V TGCA 1 cut(s) 460
HpyF10VI GCNNNNNNNGC 4 cut(s) 212, 278, 352, 407
HpyF3I CTNAG 3 cut(s) 21, 324, 638
HpySE526I ACGT 1 cut(s) 453
KspI CCGCGG 1 cut(s) 350
LmnI GCTCC 2 cut(s) 11, 236
MaeI CTAG 2 cut(s) 192, 693
MaeII ACGT 1 cut(s) 453
MaeIII GTNAC 1 cut(s) 371
MboII GAAGA 3 cut(s) 100, 263, 344
MfeI CAATTG 1 cut(s) 402
MhlI GDGCHC 2 cut(s) 8, 152
MluCI AATT 4 cut(s) 112, 164, 402, 462
MlyI GAGTC 2 cut(s) 227, 329
MmeI TCCRAC 1 cut(s) 201
MnlI CCTC 7 cut(s) 30, 271, 366, 400, 466, 558, 612
MroXI GAANNNNTTC 1 cut(s) 312
MseI TTAA 2 cut(s) 465, 621
MspA1I CMGCKG 1 cut(s) 349
MspI CCGG 3 cut(s) 65, 269, 397
MspR9I CCNGG 2 cut(s) 136, 398
MunI CAATTG 1 cut(s) 402
Mva1269I GAATGC 2 cut(s) 540, 558
MvaI CCWGG 1 cut(s) 136
MvnI CGCG 1 cut(s) 349
MwoI GCNNNNNNNGC 4 cut(s) 212, 278, 352, 407
NciI CCSGG 1 cut(s) 398
NlaIV GGNNCC 3 cut(s) 7, 238, 477
NmeAIII GCCGAG 1 cut(s) 241
NmuCI GTSAC 1 cut(s) 371
PctI GAATGC 2 cut(s) 540, 558
PdmI GAANNNNTTC 1 cut(s) 312
PinAI ACCGGT 1 cut(s) 64
PkrI GCNGC 5 cut(s) 88, 184, 345, 348, 412
PleI GAGTC 2 cut(s) 226, 328
PpsI GAGTC 2 cut(s) 226, 328
Psp124BI GAGCTC 1 cut(s) 152
Psp6I CCWGG 1 cut(s) 134
PspGI CCWGG 1 cut(s) 134
PspN4I GGNNCC 3 cut(s) 7, 238, 477
PspPI GGNCC 1 cut(s) 159
PstNI CAGNNNCTG 1 cut(s) 28
RsaI GTAC 2 cut(s) 636, 671
RsaNI GTAC 2 cut(s) 635, 670
SacI GAGCTC 1 cut(s) 152
SacII CCGCGG 1 cut(s) 350
SaqAI TTAA 2 cut(s) 465, 621
SatI GCNGC 5 cut(s) 87, 183, 344, 347, 411
Sau96I GGNCC 1 cut(s) 159
SchI GAGTC 2 cut(s) 227, 329
ScrFI CCNGG 2 cut(s) 136, 398
SduI GDGCHC 2 cut(s) 8, 152
Sfr303I CCGCGG 1 cut(s) 350
SgrBI CCGCGG 1 cut(s) 350
SinI GGWCC 1 cut(s) 159
Sse9I AATT 4 cut(s) 112, 164, 402, 462
SsiI CCGC 7 cut(s) 87, 183, 233, 344, 347, 349, 410
SspMI CTAG 2 cut(s) 192, 693
SstI GAGCTC 1 cut(s) 152
StyD4I CCNGG 2 cut(s) 134, 396
StyI CCWWGG 1 cut(s) 692
TaiI ACGT 1 cut(s) 456
TaqI TCGA 3 cut(s) 146, 152, 701
TaqII GACCGA 1 cut(s) 90
TasI AATT 4 cut(s) 112, 164, 402, 462
TatI WGTACW 1 cut(s) 669
TauI GCSGC 5 cut(s) 89, 185, 346, 349, 413
Tru1I TTAA 2 cut(s) 465, 621
Tru9I TTAA 2 cut(s) 465, 621
TscAI CASTG 1 cut(s) 124
TseFI GTSAC 1 cut(s) 371
Tsp45I GTSAC 1 cut(s) 371
TspDTI ATGAA 3 cut(s) 132, 264, 533
TspRI CASTG 1 cut(s) 124
VpaK11BI GGWCC 1 cut(s) 159
XagI CCTNNNNNAGG 1 cut(s) 20
XapI RAATTY 1 cut(s) 164
XbaI TCTAGA 1 cut(s) 191
XmaJI CCTAGG 1 cut(s) 692
XmnI GAANNNNTTC 1 cut(s) 312
XspI CTAG 2 cut(s) 192, 693
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.