FvH4_5g20230

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0007685
Physical Location & Seq
Forward (+)
11938368 .. 11940880
2513 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g20230.t1

Sequence Viewer

Length: 462 bp
ATGAATAAGATATTGGATTTCGGAAGGAAAGCTCTGTTCTACGCCAGGGTTCTTTCCGGCTATGAAGAACGGAGAATCCGATCCTATCGCCTCATGTGCGAGCAGCGTCTCAAACAGGCAGAAGAGCGGAGGCTGGCTATAAAAAAGCTTCCTGAGCAGACTATTTTGTCAGAGGTTCGCCGTATGGTTGAGGAGATGCAGAATTTAAATAAGCAGCTAGAAGAGACTGAGGCTGCTATTGAAGAGTACTTCAAGCCACTCGACCAAGAGGCTCAGAGGTTAATGAAAACACAACTTGAAGGAGAGGAGAGAACAATGAAACAGATGATGAAAACCATGCATGACCAGGCTATGCTTGATATAGCTGATGAGGAGAGAAAAGTTGAAGCACAAATAGTCGACACAGACCAGAAGAACAAAGACCCAAGACCTAATACTCAACATCTGCCACCGCCCACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

154

Amino Acids

18.3

Weight (kDa)

6.22

Isoelectric Point (pI)

61.59

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 166
AccBSI CCGCTC 1 cut(s) 127
AccI GTMKAC 1 cut(s) 399
AciI CCGC 2 cut(s) 127, 452
AclWI GGATC 1 cut(s) 75
AcsI RAATTY 1 cut(s) 202
AfaI GTAC 1 cut(s) 248
AgsI TTSAA 4 cut(s) 242, 253, 299, 386
AjnI CCWGG 2 cut(s) 44, 345
AjuI GAANNNNNNNTTGG 1 cut(s) 28
AloI GAACNNNNNNTCC 2 cut(s) 60, 92
AluBI AGCT 4 cut(s) 32, 148, 217, 365
AluI AGCT 4 cut(s) 32, 148, 217, 365
Alw26I GTCTC 2 cut(s) 113, 218
AlwI GGATC 1 cut(s) 75
ApeKI GCWGC 3 cut(s) 103, 214, 233
ApoI RAATTY 1 cut(s) 202
BbvI GCAGC 3 cut(s) 115, 220, 226
BceAI ACGGC 1 cut(s) 165
BciT130I CCWGG 2 cut(s) 46, 347
BcoDI GTCTC 2 cut(s) 113, 218
BfaI CTAG 1 cut(s) 218
BisI GCNGC 3 cut(s) 104, 215, 234
BlsI GCNGC 3 cut(s) 105, 216, 235
BmcAI AGTACT 1 cut(s) 248
Bme1390I CCNGG 2 cut(s) 46, 347
BmrFI CCNGG 2 cut(s) 46, 347
BmsI GCATC 1 cut(s) 186
Bpu10I CCTNAGC 1 cut(s) 153
BsaJI CCNNGG 1 cut(s) 45
BseBI CCWGG 2 cut(s) 46, 347
BseDI CCNNGG 1 cut(s) 45
BseMII CTCAG 3 cut(s) 144, 219, 287
BseRI GAGGAG 3 cut(s) 206, 320, 386
BseXI GCAGC 3 cut(s) 115, 220, 226
BsiSI CCGG 1 cut(s) 57
BsmAI GTCTC 2 cut(s) 113, 218
BsmBI CGTCTC 1 cut(s) 113
Bsp143I GATC 1 cut(s) 80
BspACI CCGC 2 cut(s) 127, 452
BspCNI CTCAG 3 cut(s) 145, 220, 286
BspPI GGATC 1 cut(s) 75
BspQI GCTCTTC 1 cut(s) 117
BsrBI CCGCTC 1 cut(s) 127
BssECI CCNNGG 1 cut(s) 45
BssMI GATC 1 cut(s) 80
Bst2UI CCWGG 2 cut(s) 46, 347
Bst6I CTCTTC 3 cut(s) 117, 216, 237
BstC8I GCNNGC 2 cut(s) 101, 135
BstDEI CTNAG 3 cut(s) 153, 228, 273
BstKTI GATC 1 cut(s) 83
BstMAI GTCTC 2 cut(s) 113, 218
BstMBI GATC 1 cut(s) 80
BstMWI GCNNNNNNNGC 2 cut(s) 96, 154
BstNI CCWGG 2 cut(s) 46, 347
BstSCI CCNGG 2 cut(s) 44, 345
BstV1I GCAGC 3 cut(s) 115, 220, 226
Cac8I GCNNGC 2 cut(s) 101, 135
CseI GACGC 1 cut(s) 95
Csp6I GTAC 1 cut(s) 247
CviAII CATG 4 cut(s) 94, 337, 341, 459
CviQI GTAC 1 cut(s) 247
DdeI CTNAG 3 cut(s) 153, 228, 273
DpnI GATC 1 cut(s) 82
DpnII GATC 1 cut(s) 80
DraI TTTAAA 1 cut(s) 207
DrdI GACNNNNNNGTC 1 cut(s) 166
DseDI GACNNNNNNGTC 1 cut(s) 166
Eam1104I CTCTTC 3 cut(s) 117, 216, 237
EarI CTCTTC 3 cut(s) 117, 216, 237
EcoRII CCWGG 2 cut(s) 44, 345
EcoT22I ATGCAT 1 cut(s) 342
Esp3I CGTCTC 1 cut(s) 113
FaeI CATG 4 cut(s) 97, 340, 344, 462
FaiI YATR 9 cut(s) 63, 95, 140, 185, 338, 342, 353, 362, 460
FatI CATG 4 cut(s) 93, 336, 340, 458
FblI GTMKAC 1 cut(s) 399
Fnu4HI GCNGC 3 cut(s) 104, 215, 234
Fsp4HI GCNGC 3 cut(s) 104, 215, 234
FspBI CTAG 1 cut(s) 218
GluI GCNGC 3 cut(s) 104, 215, 234
HapII CCGG 1 cut(s) 57
HgaI GACGC 1 cut(s) 95
Hin1II CATG 4 cut(s) 97, 340, 344, 462
HincII GTYRAC 1 cut(s) 400
HindII GTYRAC 1 cut(s) 400
HindIII AAGCTT 1 cut(s) 146
HinfI GANTC 1 cut(s) 75
HpaII CCGG 1 cut(s) 57
Hpy166II GTNNAC 1 cut(s) 400
Hpy188I TCNGA 4 cut(s) 23, 80, 172, 276
Hpy188III TCNNGA 1 cut(s) 152
Hpy8I GTNNAC 1 cut(s) 400
HpyAV CCTTC 2 cut(s) 18, 293
HpyCH4V TGCA 2 cut(s) 199, 340
HpyF10VI GCNNNNNNNGC 2 cut(s) 96, 154
HpyF3I CTNAG 3 cut(s) 153, 228, 273
Hsp92II CATG 4 cut(s) 97, 340, 344, 462
Kzo9I GATC 1 cut(s) 80
LguI GCTCTTC 1 cut(s) 117
LpnPI CCDG 9 cut(s) 31, 58, 70, 101, 119, 165, 332, 359, 422
Lsp1109I GCAGC 3 cut(s) 115, 220, 226
LweI GCATC 1 cut(s) 186
MaeI CTAG 1 cut(s) 218
MalI GATC 1 cut(s) 82
MbiI CCGCTC 1 cut(s) 127
MboI GATC 1 cut(s) 80
MboII GAAGA 5 cut(s) 77, 134, 233, 254, 424
MluCI AATT 1 cut(s) 202
MnlI CCTC 9 cut(s) 101, 123, 166, 184, 223, 262, 270, 298, 364
Mph1103I ATGCAT 1 cut(s) 342
MseI TTAA 2 cut(s) 206, 281
MspI CCGG 1 cut(s) 57
MspR9I CCNGG 2 cut(s) 46, 347
MvaI CCWGG 2 cut(s) 46, 347
MwoI GCNNNNNNNGC 2 cut(s) 96, 154
NdeII GATC 1 cut(s) 80
NlaIII CATG 4 cut(s) 97, 340, 344, 462
NsiI ATGCAT 1 cut(s) 342
PciSI GCTCTTC 1 cut(s) 117
PcsI WCGNNNNNNNCGW 1 cut(s) 76
PfeI GAWTC 1 cut(s) 75
PkrI GCNGC 3 cut(s) 105, 216, 235
Psp6I CCWGG 2 cut(s) 44, 345
PspGI CCWGG 2 cut(s) 44, 345
RsaI GTAC 1 cut(s) 248
RsaNI GTAC 1 cut(s) 247
SalI GTCGAC 1 cut(s) 398
SapI GCTCTTC 1 cut(s) 117
SaqAI TTAA 2 cut(s) 206, 281
SatI GCNGC 3 cut(s) 104, 215, 234
Sau3AI GATC 1 cut(s) 80
ScaI AGTACT 1 cut(s) 248
ScrFI CCNGG 2 cut(s) 46, 347
SetI ASST 7 cut(s) 34, 150, 177, 219, 281, 367, 433
SfaNI GCATC 1 cut(s) 186
SmiI ATTTAAAT 1 cut(s) 207
Sse9I AATT 1 cut(s) 202
SsiI CCGC 2 cut(s) 127, 452
SspMI CTAG 1 cut(s) 218
StyD4I CCNGG 2 cut(s) 44, 345
SwaI ATTTAAAT 1 cut(s) 207
TaqI TCGA 2 cut(s) 261, 399
TasI AATT 1 cut(s) 202
TatI WGTACW 1 cut(s) 246
TfiI GAWTC 1 cut(s) 75
Tru1I TTAA 2 cut(s) 206, 281
Tru9I TTAA 2 cut(s) 206, 281
TseI GCWGC 3 cut(s) 103, 214, 233
TspDTI ATGAA 5 cut(s) 17, 78, 299, 332, 344
TspGWI ACGGA 1 cut(s) 85
XapI RAATTY 1 cut(s) 202
XmiI GTMKAC 1 cut(s) 399
XspI CTAG 1 cut(s) 218
ZrmI AGTACT 1 cut(s) 248
Zsp2I ATGCAT 1 cut(s) 342
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.