MD04G1051900.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0007685
Physical Location & Seq
Forward (+)
6090173 .. 6093137
2965 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1051900.v1.1.491

Sequence Viewer

Length: 459 bp
ATGAACAAGCTGATTGAATTCGGAAAGAAAGCTCTGTTCTATGCGAGGGTTCTTTCGGGCTACGAAGAACGGAGAATCAGATCGTTTCGATTGGAAGTTGAAAAGCGCCTAAAAGAGGCAAATGCTCGGAAGTTAGCCTTAAGAAAGATCCCTGAACAAACTATATTATCGGAGGTTCGCCGTATGGTTGAGGAGATGCAAACTTTAAACAGGAAGCTGGAAGAAACGGAGGCTCAAATTGAAGAGTACTTCAAGCCGATTGACAAAGAGGCTGAGATGATTATGAAAACACAACTTGAAGGAGAGGAGAGGACCATGAAGGAGATGATGAAAGCCATGCATCAGCAGGCTTTGCTCGAGAAAGCTGAAGCGGAGAAAACCGCACAAAAGAAATTGGAAACAAACCAGAAGAACCAAGAGTCGATACCCAAGACACAGCATCTGCCACCGCCCCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

153

Amino Acids

18.04

Weight (kDa)

9.2

Isoelectric Point (pI)

52.85

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 371, 381, 449
AclWI GGATC 1 cut(s) 142
AcsI RAATTY 1 cut(s) 17
AcuI CTGAAG 1 cut(s) 387
AfaI GTAC 1 cut(s) 248
AfiI CCNNNNNNNGG 1 cut(s) 115
AflII CTTAAG 1 cut(s) 139
AgsI TTSAA 5 cut(s) 17, 101, 242, 253, 299
AluBI AGCT 4 cut(s) 10, 32, 217, 365
AluI AGCT 4 cut(s) 10, 32, 217, 365
AlwI GGATC 1 cut(s) 142
AlwNI CAGNNNCTG 1 cut(s) 442
Ama87I CYCGRG 1 cut(s) 356
ApoI RAATTY 1 cut(s) 17
AspLEI GCGC 1 cut(s) 108
AspS9I GGNCC 1 cut(s) 312
AvaI CYCGRG 1 cut(s) 356
AvaII GGWCC 1 cut(s) 312
BceAI ACGGC 1 cut(s) 165
BfoI RGCGCY 1 cut(s) 109
BfrI CTTAAG 1 cut(s) 139
BmcAI AGTACT 1 cut(s) 248
Bme18I GGWCC 1 cut(s) 312
BmeT110I CYCGRG 1 cut(s) 356
BmgT120I GGNCC 1 cut(s) 312
BmsI GCATC 3 cut(s) 186, 349, 448
Bsc4I CCNNNNNNNGG 1 cut(s) 115
BseLI CCNNNNNNNGG 1 cut(s) 115
BseMII CTCAG 1 cut(s) 264
BseRI GAGGAG 2 cut(s) 206, 320
BsiHKCI CYCGRG 1 cut(s) 356
BslI CCNNNNNNNGG 1 cut(s) 115
BsoBI CYCGRG 1 cut(s) 356
Bsp143I GATC 2 cut(s) 80, 147
BspACI CCGC 3 cut(s) 371, 381, 449
BspCNI CTCAG 1 cut(s) 265
BspPI GGATC 1 cut(s) 142
BspTI CTTAAG 1 cut(s) 139
BssMI GATC 2 cut(s) 80, 147
Bst6I CTCTTC 1 cut(s) 237
BstAFI CTTAAG 1 cut(s) 139
BstAPI GCANNNNNTGC 1 cut(s) 352
BstC8I GCNNGC 1 cut(s) 348
BstDEI CTNAG 1 cut(s) 273
BstENI CCTNNNNNAGG 1 cut(s) 113
BstH2I RGCGCY 1 cut(s) 109
BstHHI GCGC 1 cut(s) 108
BstKTI GATC 2 cut(s) 83, 150
BstMBI GATC 2 cut(s) 80, 147
BstMWI GCNNNNNNNGC 1 cut(s) 352
BstX2I RGATCY 1 cut(s) 147
BstYI RGATCY 1 cut(s) 147
Cac8I GCNNGC 1 cut(s) 348
CaiI CAGNNNCTG 1 cut(s) 442
CfoI GCGC 1 cut(s) 108
Cfr13I GGNCC 1 cut(s) 312
Csp6I GTAC 1 cut(s) 247
CviAII CATG 3 cut(s) 316, 337, 456
CviQI GTAC 1 cut(s) 247
DdeI CTNAG 1 cut(s) 273
DpnI GATC 2 cut(s) 82, 149
DpnII GATC 2 cut(s) 80, 147
DraI TTTAAA 1 cut(s) 207
Eam1104I CTCTTC 1 cut(s) 237
EarI CTCTTC 1 cut(s) 237
Eco47I GGWCC 1 cut(s) 312
Eco57I CTGAAG 1 cut(s) 387
Eco88I CYCGRG 1 cut(s) 356
EcoNI CCTNNNNNAGG 1 cut(s) 113
EcoRI GAATTC 1 cut(s) 17
EcoT22I ATGCAT 1 cut(s) 342
FaeI CATG 3 cut(s) 319, 340, 459
FaiI YATR 7 cut(s) 42, 164, 185, 284, 317, 338, 457
FalI AAGNNNNNCTT 2 cut(s) 122, 154
FatI CATG 3 cut(s) 315, 336, 455
GlaI GCGC 1 cut(s) 107
HaeII RGCGCY 1 cut(s) 109
HhaI GCGC 1 cut(s) 108
Hin1II CATG 3 cut(s) 319, 340, 459
Hin6I GCGC 1 cut(s) 106
HinP1I GCGC 1 cut(s) 106
HinfI GANTC 2 cut(s) 75, 419
Hpy188I TCNGA 4 cut(s) 23, 80, 129, 172
Hpy188III TCNNGA 1 cut(s) 358
HpyAV CCTTC 2 cut(s) 293, 313
HpyCH4V TGCA 2 cut(s) 199, 340
HpyF10VI GCNNNNNNNGC 1 cut(s) 352
HpyF3I CTNAG 1 cut(s) 273
Hsp92II CATG 3 cut(s) 319, 340, 459
HspAI GCGC 1 cut(s) 106
Kzo9I GATC 2 cut(s) 80, 147
LpnPI CCDG 5 cut(s) 165, 196, 203, 332, 419
LweI GCATC 3 cut(s) 186, 349, 448
MalI GATC 2 cut(s) 82, 149
MboI GATC 2 cut(s) 80, 147
MboII GAAGA 4 cut(s) 77, 233, 254, 421
MflI RGATCY 1 cut(s) 147
MluCI AATT 3 cut(s) 17, 237, 392
MlyI GAGTC 1 cut(s) 428
MnlI CCTC 8 cut(s) 39, 109, 166, 184, 223, 262, 298, 303
Mph1103I ATGCAT 1 cut(s) 342
MseI TTAA 2 cut(s) 140, 206
MspCI CTTAAG 1 cut(s) 139
MwoI GCNNNNNNNGC 1 cut(s) 352
NdeII GATC 2 cut(s) 80, 147
NlaIII CATG 3 cut(s) 319, 340, 459
NsiI ATGCAT 1 cut(s) 342
PaeR7I CTCGAG 1 cut(s) 356
PfeI GAWTC 1 cut(s) 75
PleI GAGTC 1 cut(s) 427
PpsI GAGTC 1 cut(s) 427
PspPI GGNCC 1 cut(s) 312
PstNI CAGNNNCTG 1 cut(s) 442
PsuI RGATCY 1 cut(s) 147
RsaI GTAC 1 cut(s) 248
RsaNI GTAC 1 cut(s) 247
SaqAI TTAA 2 cut(s) 140, 206
Sau3AI GATC 2 cut(s) 80, 147
Sau96I GGNCC 1 cut(s) 312
ScaI AGTACT 1 cut(s) 248
SchI GAGTC 1 cut(s) 428
SetI ASST 5 cut(s) 12, 34, 177, 219, 367
SfaNI GCATC 3 cut(s) 186, 349, 448
Sfr274I CTCGAG 1 cut(s) 356
SinI GGWCC 1 cut(s) 312
SlaI CTCGAG 1 cut(s) 356
SmlI CTYRAG 2 cut(s) 139, 356
SmoI CTYRAG 2 cut(s) 139, 356
Sse9I AATT 3 cut(s) 17, 237, 392
SsiI CCGC 3 cut(s) 371, 381, 449
TaqI TCGA 3 cut(s) 88, 357, 422
TasI AATT 3 cut(s) 17, 237, 392
TatI WGTACW 1 cut(s) 246
TfiI GAWTC 1 cut(s) 75
Tru1I TTAA 2 cut(s) 140, 206
Tru9I TTAA 2 cut(s) 140, 206
TspDTI ATGAA 4 cut(s) 17, 299, 332, 344
TspGWI ACGGA 2 cut(s) 85, 242
Vha464I CTTAAG 1 cut(s) 139
VpaK11BI GGWCC 1 cut(s) 312
XagI CCTNNNNNAGG 1 cut(s) 113
XapI RAATTY 1 cut(s) 17
XhoI CTCGAG 1 cut(s) 356
ZrmI AGTACT 1 cut(s) 248
Zsp2I ATGCAT 1 cut(s) 342
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.