MD06G1048200.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0007685
Physical Location & Seq
Reverse (-)
6526894 .. 6536200
9307 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1048200.v1.1.491

Sequence Viewer

Length: 459 bp
ATGAATAAGCTGATTGAATTCGGAAAGAAAGCTCTGTTCTATGCGAGGGTTCTTTCCGGCTACGAAGAACGGAGAATCCGATCGTTTCGGTTGGAGGTTGAAAAGCGCTTGAAAGAGGCAAATGCGCGGAAGTTAGCCTTAAGAAAGATTCCTGAACAAACTATATTATCGGAGGTTCGCCGTATGGTCGAGGAGATGCAAACTTTAAATAGGAAGCTGGAAGAAACTGAGGCTCAAATTGAAGAGTACTTCAAGCCGATTGACAAAGAGGCTGAGATGATTATGAAAACGCAACTTGAAGGAGAGGAGAGGACAATGAAGGAGATGATGAAAGCCATGCATCAGCAGGCTTTGCTTGAGAAAACAGAAGCGGAGAAAGAAGCACAAAAGAAATTGGAAACAGACCGGAAGAACCAAGAGTCAAGACCCAAGACCCAGCATCTGCCGCCGCCCCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

153

Amino Acids

18.17

Weight (kDa)

9.2

Isoelectric Point (pI)

57.83

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 127
AciI CCGC 4 cut(s) 127, 371, 446, 449
AcsI RAATTY 1 cut(s) 17
AfaI GTAC 1 cut(s) 248
AfeI AGCGCT 1 cut(s) 107
AflII CTTAAG 1 cut(s) 139
AgsI TTSAA 6 cut(s) 17, 101, 112, 242, 253, 299
AloI GAACNNNNNNTCC 2 cut(s) 60, 92
AluBI AGCT 3 cut(s) 10, 32, 217
AluI AGCT 3 cut(s) 10, 32, 217
AlwNI CAGNNNCTG 1 cut(s) 442
Aor51HI AGCGCT 1 cut(s) 107
ApoI RAATTY 1 cut(s) 17
AspLEI GCGC 2 cut(s) 108, 127
BceAI ACGGC 1 cut(s) 165
BfoI RGCGCY 1 cut(s) 109
BfrI CTTAAG 1 cut(s) 139
BisI GCNGC 2 cut(s) 446, 449
BlsI GCNGC 2 cut(s) 447, 450
BmcAI AGTACT 1 cut(s) 248
BmsI GCATC 3 cut(s) 186, 349, 448
BpuEI CTTGAG 1 cut(s) 377
BsaWI WCCGGW 1 cut(s) 405
BseMII CTCAG 2 cut(s) 219, 264
BseRI GAGGAG 2 cut(s) 206, 320
BseYI CCCAGC 1 cut(s) 435
Bsh1236I CGCG 1 cut(s) 127
Bsh1285I CGRYCG 1 cut(s) 83
BsiEI CGRYCG 1 cut(s) 83
BsiSI CCGG 2 cut(s) 57, 406
Bsp143I GATC 1 cut(s) 80
BspACI CCGC 4 cut(s) 127, 371, 446, 449
BspCNI CTCAG 2 cut(s) 220, 265
BspFNI CGCG 1 cut(s) 127
BspTI CTTAAG 1 cut(s) 139
BssMI GATC 1 cut(s) 80
Bst6I CTCTTC 1 cut(s) 237
BstAFI CTTAAG 1 cut(s) 139
BstAPI GCANNNNNTGC 1 cut(s) 352
BstC8I GCNNGC 1 cut(s) 348
BstDEI CTNAG 2 cut(s) 228, 273
BstFNI CGCG 1 cut(s) 127
BstH2I RGCGCY 1 cut(s) 109
BstHHI GCGC 2 cut(s) 108, 127
BstKTI GATC 1 cut(s) 83
BstMBI GATC 1 cut(s) 80
BstMCI CGRYCG 1 cut(s) 83
BstMWI GCNNNNNNNGC 2 cut(s) 352, 445
BstUI CGCG 1 cut(s) 127
Cac8I GCNNGC 1 cut(s) 348
CaiI CAGNNNCTG 1 cut(s) 442
CfoI GCGC 2 cut(s) 108, 127
Csp6I GTAC 1 cut(s) 247
CviAII CATG 1 cut(s) 337
CviQI GTAC 1 cut(s) 247
DdeI CTNAG 2 cut(s) 228, 273
DpnI GATC 1 cut(s) 82
DpnII GATC 1 cut(s) 80
DraI TTTAAA 1 cut(s) 207
Eam1104I CTCTTC 1 cut(s) 237
EarI CTCTTC 1 cut(s) 237
Eco47III AGCGCT 1 cut(s) 107
EcoRI GAATTC 1 cut(s) 17
EcoT22I ATGCAT 1 cut(s) 342
FaeI CATG 1 cut(s) 340
FaiI YATR 5 cut(s) 42, 164, 185, 284, 338
FalI AAGNNNNNCTT 2 cut(s) 122, 154
FatI CATG 1 cut(s) 336
Fnu4HI GCNGC 2 cut(s) 446, 449
Fsp4HI GCNGC 2 cut(s) 446, 449
GlaI GCGC 2 cut(s) 107, 126
GluI GCNGC 2 cut(s) 446, 449
GsaI CCCAGC 1 cut(s) 439
HaeII RGCGCY 1 cut(s) 109
HapII CCGG 2 cut(s) 57, 406
HhaI GCGC 2 cut(s) 108, 127
Hin1II CATG 1 cut(s) 340
Hin6I GCGC 2 cut(s) 106, 125
HinP1I GCGC 2 cut(s) 106, 125
HinfI GANTC 3 cut(s) 75, 148, 419
HpaII CCGG 2 cut(s) 57, 406
Hpy188I TCNGA 3 cut(s) 23, 80, 172
Hpy188III TCNNGA 2 cut(s) 152, 423
HpyAV CCTTC 2 cut(s) 293, 313
HpyCH4V TGCA 2 cut(s) 199, 340
HpyF10VI GCNNNNNNNGC 2 cut(s) 352, 445
HpyF3I CTNAG 2 cut(s) 228, 273
Hsp92II CATG 1 cut(s) 340
HspAI GCGC 2 cut(s) 106, 125
Kzo9I GATC 1 cut(s) 80
LpnPI CCDG 6 cut(s) 70, 165, 203, 332, 419, 449
LweI GCATC 3 cut(s) 186, 349, 448
MalI GATC 1 cut(s) 82
MboI GATC 1 cut(s) 80
MboII GAAGA 4 cut(s) 77, 233, 254, 421
MluCI AATT 3 cut(s) 17, 237, 392
MlyI GAGTC 1 cut(s) 428
MmeI TCCRAC 1 cut(s) 72
MnlI CCTC 9 cut(s) 39, 88, 109, 166, 184, 223, 262, 298, 303
Mph1103I ATGCAT 1 cut(s) 342
MseI TTAA 2 cut(s) 140, 206
MspCI CTTAAG 1 cut(s) 139
MspI CCGG 2 cut(s) 57, 406
MvnI CGCG 1 cut(s) 127
MwoI GCNNNNNNNGC 2 cut(s) 352, 445
NdeII GATC 1 cut(s) 80
NlaIII CATG 1 cut(s) 340
NsiI ATGCAT 1 cut(s) 342
PcsI WCGNNNNNNNCGW 1 cut(s) 76
PfeI GAWTC 2 cut(s) 75, 148
PkrI GCNGC 2 cut(s) 447, 450
Ple19I CGATCG 1 cut(s) 83
PleI GAGTC 1 cut(s) 427
PpsI GAGTC 1 cut(s) 427
PspFI CCCAGC 1 cut(s) 435
PstNI CAGNNNCTG 1 cut(s) 442
PvuI CGATCG 1 cut(s) 83
RsaI GTAC 1 cut(s) 248
RsaNI GTAC 1 cut(s) 247
SaqAI TTAA 2 cut(s) 140, 206
SatI GCNGC 2 cut(s) 446, 449
Sau3AI GATC 1 cut(s) 80
ScaI AGTACT 1 cut(s) 248
SchI GAGTC 1 cut(s) 428
SetI ASST 5 cut(s) 12, 34, 99, 177, 219
SfaNI GCATC 3 cut(s) 186, 349, 448
SmlI CTYRAG 2 cut(s) 139, 356
SmoI CTYRAG 2 cut(s) 139, 356
Sse9I AATT 3 cut(s) 17, 237, 392
SsiI CCGC 4 cut(s) 127, 371, 446, 449
TaqI TCGA 1 cut(s) 189
TasI AATT 3 cut(s) 17, 237, 392
TatI WGTACW 1 cut(s) 246
TauI GCSGC 2 cut(s) 448, 451
TfiI GAWTC 2 cut(s) 75, 148
Tru1I TTAA 2 cut(s) 140, 206
Tru9I TTAA 2 cut(s) 140, 206
TspDTI ATGAA 4 cut(s) 17, 299, 332, 344
TspGWI ACGGA 1 cut(s) 85
Vha464I CTTAAG 1 cut(s) 139
XapI RAATTY 1 cut(s) 17
ZrmI AGTACT 1 cut(s) 248
Zsp2I ATGCAT 1 cut(s) 342
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.