FvH4_5g20310

ATP-dependent Clp protease proteolytic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0009609
Physical Location & Seq
Reverse (-)
11991105 .. 11996223
5119 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g20310.t1

Sequence Viewer

Length: 906 bp
ATGGAGGTTTCCGCCACCTCAGCTTCCAGCTTCTCGCTTCACGCGCGCCTCTCAGCTCCTCGAGCCCTAAATCCAAACCGCATCCAAACGATGTCGTCTTGTTCGCCTCTCAGAGCCTCCCTCTCCTCCAACTTCCTCTCGCCCTCCGCCGGCGGCAGCGTCTCCTCAGACCTCTCCGGCTTCAAGCTCCGCCCTTCCTCTCTCAATCCGGCGTCGTATCGTGGCTCTAAGCCCAAACGCGGTGTCGTCACCATGGTTATTCCATTTCAAAGAGGAAGTGCGTGGGAGCAACCACCTCCGGACCTGGCATCGTATTTATACAAGAACCGAATTGTTTACTTGGGAATGTCGCTTGTTCCTTCAGTAACTGAGTTGATTCTTGCAGAGTTTCTCTACCTTCAGTACGAGGACGATACGAAGCCTATCTACTTGTACATAAATTCCACTGGAACAACCAAGGGTGGTGAGAAATTGGGGTACGAGACAGAAGCTCTTGCCATATATGATGTCATGCGGTATGTCAAGCCACCGATATTTACTCTTTGTGTTGGTAATGCTTGGGGAGAAGCCGCTTTACTCTTGGCTGCTGGTGCAAAGGGAAACCGTTCTGCGTTACCCTCATCAACAATCATGATAAAGCAGCCAATTGCAAGGTTCCAAGGTCAAGCAACAGATATTGAACTTGCAAGAAAAGAAGTGAAGAATGTGAAAGACGAGTTGGTCAAGCTCTTTGCTAAGCATGTTGGAAAATCGACCGAGCAAATTGAAGCAGACATTAGACGTCCAAAATATTTCAGTCCCAGCGAGGCAGTTGAATATGGCATCATAGACAAGGTACTATACAATGAAAGGGGTCATAAAGACAGGGGAGTAGTCGCTGACCTGAAAAAGGCACAGCTTATTTGA

Protein Analysis

302

Amino Acids

32.99

Weight (kDa)

9.44

Isoelectric Point (pI)

35.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CLP_protease PF00574 101 - 281 2.7e-52 Clp protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 719
AatII GACGTC 1 cut(s) 784
AccII CGCG 3 cut(s) 44, 46, 240
AccIII TCCGGA 1 cut(s) 298
AciI CCGC 8 cut(s) 12, 79, 147, 153, 190, 240, 514, 570
AcsI RAATTY 1 cut(s) 439
AcuI CTGAAG 2 cut(s) 345, 383
AcyI GRCGYC 2 cut(s) 212, 781
AfaI GTAC 4 cut(s) 404, 434, 479, 837
AfiI CCNNNNNNNGG 3 cut(s) 149, 239, 889
AgsI TTSAA 5 cut(s) 184, 269, 680, 767, 815
AjnI CCWGG 1 cut(s) 303
AjuI GAANNNNNNNTTGG 2 cut(s) 701, 733
AluBI AGCT 7 cut(s) 23, 30, 56, 187, 491, 727, 898
AluI AGCT 7 cut(s) 23, 30, 56, 187, 491, 727, 898
Alw26I GTCTC 2 cut(s) 166, 476
AlwNI CAGNNNCTG 1 cut(s) 368
Ama87I CYCGRG 1 cut(s) 60
Aor13HI TCCGGA 1 cut(s) 298
ApeKI GCWGC 3 cut(s) 156, 584, 640
ApoI RAATTY 1 cut(s) 439
ArsI GACNNNNNNTTYG 2 cut(s) 228, 260
Asp700I GAANNNNTTC 1 cut(s) 604
AspLEI GCGC 2 cut(s) 46, 48
AspS9I GGNCC 1 cut(s) 301
AsuHPI GGTGA 2 cut(s) 241, 476
AvaI CYCGRG 1 cut(s) 60
AvaII GGWCC 1 cut(s) 301
BanII GRGCYC 1 cut(s) 67
BarI GAAGNNNNNNTAC 4 cut(s) 410, 442, 558, 590
BbvCI CCTCAGC 1 cut(s) 19
BbvI GCAGC 3 cut(s) 168, 571, 652
BciT130I CCWGG 1 cut(s) 305
BcoDI GTCTC 2 cut(s) 166, 476
BisI GCNGC 5 cut(s) 154, 157, 570, 585, 641
BlpI GCTNAGC 1 cut(s) 735
BlsI GCNGC 5 cut(s) 155, 158, 571, 586, 642
Bme1390I CCNGG 1 cut(s) 305
Bme18I GGWCC 1 cut(s) 301
BmeT110I CYCGRG 1 cut(s) 60
BmgT120I GGNCC 1 cut(s) 301
BmiI GGNNCC 1 cut(s) 656
BmrFI CCNGG 1 cut(s) 305
BmsI GCATC 3 cut(s) 90, 317, 831
BplI GAGNNNNNCTC 2 cut(s) 105, 137
Bpu10I CCTNAGC 1 cut(s) 19
Bpu1102I GCTNAGC 1 cut(s) 735
BsaHI GRCGYC 2 cut(s) 212, 781
BsaJI CCNNGG 3 cut(s) 252, 456, 658
BsaWI WCCGGW 1 cut(s) 298
Bsc4I CCNNNNNNNGG 3 cut(s) 149, 239, 889
Bse118I RCCGGY 1 cut(s) 149
Bse1I ACTGG 1 cut(s) 451
BseAI TCCGGA 1 cut(s) 298
BseBI CCWGG 1 cut(s) 305
BseDI CCNNGG 3 cut(s) 252, 456, 658
BseGI GGATG 1 cut(s) 81
BseLI CCNNNNNNNGG 3 cut(s) 149, 239, 889
BseMII CTCAG 5 cut(s) 33, 66, 124, 180, 360
BseNI ACTGG 1 cut(s) 451
BsePI GCGCGC 1 cut(s) 44
BseRI GAGGAG 3 cut(s) 48, 115, 154
BseXI GCAGC 3 cut(s) 168, 571, 652
BseYI CCCAGC 1 cut(s) 800
Bsh1236I CGCG 3 cut(s) 44, 46, 240
Bsh1285I CGRYCG 1 cut(s) 756
BsiEI CGRYCG 1 cut(s) 756
BsiHKCI CYCGRG 1 cut(s) 60
BsiSI CCGG 4 cut(s) 150, 177, 209, 299
BslFI GGGAC 1 cut(s) 783
BslI CCNNNNNNNGG 3 cut(s) 149, 239, 889
BsmAI GTCTC 2 cut(s) 166, 476
BsmBI CGTCTC 1 cut(s) 166
BsmFI GGGAC 1 cut(s) 783
BsoBI CYCGRG 1 cut(s) 60
Bsp1286I GDGCHC 1 cut(s) 67
Bsp13I TCCGGA 1 cut(s) 298
Bsp1407I TGTACA 1 cut(s) 432
Bsp1720I GCTNAGC 1 cut(s) 735
Bsp19I CCATGG 1 cut(s) 252
BspACI CCGC 8 cut(s) 12, 79, 147, 153, 190, 240, 514, 570
BspCNI CTCAG 5 cut(s) 32, 65, 123, 179, 361
BspEI TCCGGA 1 cut(s) 298
BspFNI CGCG 3 cut(s) 44, 46, 240
BspHI TCATGA 1 cut(s) 630
BspLI GGNNCC 1 cut(s) 656
BsrFI RCCGGY 1 cut(s) 149
BsrGI TGTACA 1 cut(s) 432
BsrI ACTGG 1 cut(s) 451
BssAI RCCGGY 1 cut(s) 149
BssECI CCNNGG 3 cut(s) 252, 456, 658
BssHII GCGCGC 1 cut(s) 44
BssNI GRCGYC 2 cut(s) 212, 781
BssT1I CCWWGG 3 cut(s) 252, 456, 658
Bst2UI CCWGG 1 cut(s) 305
Bst4CI ACNGT 1 cut(s) 605
BstACI GRCGYC 2 cut(s) 212, 781
BstAUI TGTACA 1 cut(s) 432
BstC8I GCNNGC 2 cut(s) 46, 151
BstDEI CTNAG 7 cut(s) 19, 52, 110, 166, 228, 369, 735
BstDSI CCRYGG 1 cut(s) 252
BstENI CCTNNNNNAGG 1 cut(s) 887
BstF5I GGATG 1 cut(s) 81
BstFNI CGCG 3 cut(s) 44, 46, 240
BstHHI GCGC 2 cut(s) 46, 48
BstMAI GTCTC 2 cut(s) 166, 476
BstMCI CGRYCG 1 cut(s) 756
BstMWI GCNNNNNNNGC 4 cut(s) 20, 43, 62, 590
BstNI CCWGG 1 cut(s) 305
BstNSI RCATGY 1 cut(s) 743
BstSCI CCNGG 1 cut(s) 303
BstUI CGCG 3 cut(s) 44, 46, 240
BstV1I GCAGC 3 cut(s) 168, 571, 652
BtgI CCRYGG 1 cut(s) 252
BtsCI GGATG 1 cut(s) 81
BtsIMutI CAGTG 1 cut(s) 444
Cac8I GCNNGC 2 cut(s) 46, 151
CaiI CAGNNNCTG 1 cut(s) 368
CciI TCATGA 1 cut(s) 630
CfoI GCGC 2 cut(s) 46, 48
Cfr10I RCCGGY 1 cut(s) 149
Cfr13I GGNCC 1 cut(s) 301
CseI GACGC 2 cut(s) 148, 201
Csp6I GTAC 4 cut(s) 403, 433, 478, 836
CviAII CATG 4 cut(s) 253, 511, 631, 740
CviQI GTAC 4 cut(s) 403, 433, 478, 836
DdeI CTNAG 7 cut(s) 19, 52, 110, 166, 228, 369, 735
DrdI GACNNNNNNGTC 1 cut(s) 719
DseDI GACNNNNNNGTC 1 cut(s) 719
EciI GGCGGA 2 cut(s) 136, 179
Eco130I CCWWGG 3 cut(s) 252, 456, 658
Eco24I GRGCYC 1 cut(s) 67
Eco47I GGWCC 1 cut(s) 301
Eco57I CTGAAG 2 cut(s) 345, 383
Eco88I CYCGRG 1 cut(s) 60
EcoNI CCTNNNNNAGG 1 cut(s) 887
EcoRII CCWGG 1 cut(s) 303
EcoT14I CCWWGG 3 cut(s) 252, 456, 658
EcoT38I GRGCYC 1 cut(s) 67
ErhI CCWWGG 3 cut(s) 252, 456, 658
Esp3I CGTCTC 1 cut(s) 166
FaeI CATG 4 cut(s) 256, 514, 634, 743
FaqI GGGAC 1 cut(s) 783
FatI CATG 4 cut(s) 252, 510, 630, 739
Fnu4HI GCNGC 5 cut(s) 154, 157, 570, 585, 641
FokI GGATG 1 cut(s) 68
FriOI GRGCYC 1 cut(s) 67
Fsp4HI GCNGC 5 cut(s) 154, 157, 570, 585, 641
GlaI GCGC 2 cut(s) 45, 47
GluI GCNGC 5 cut(s) 154, 157, 570, 585, 641
GsaI CCCAGC 1 cut(s) 804
HapII CCGG 4 cut(s) 150, 177, 209, 299
HgaI GACGC 2 cut(s) 148, 201
HhaI GCGC 2 cut(s) 46, 48
Hin1I GRCGYC 2 cut(s) 212, 781
Hin1II CATG 4 cut(s) 256, 514, 634, 743
Hin6I GCGC 2 cut(s) 44, 46
HinP1I GCGC 2 cut(s) 44, 46
HinfI GANTC 1 cut(s) 376
HpaII CCGG 4 cut(s) 150, 177, 209, 299
HphI GGTGA 2 cut(s) 241, 476
Hpy166II GTNNAC 1 cut(s) 337
Hpy188I TCNGA 2 cut(s) 113, 169
Hpy188III TCNNGA 2 cut(s) 299, 631
Hpy8I GTNNAC 1 cut(s) 337
Hpy99I CGWCG 1 cut(s) 217
HpyAV CCTTC 3 cut(s) 204, 369, 407
HpyCH4III ACNGT 1 cut(s) 605
HpyCH4IV ACGT 1 cut(s) 781
HpyCH4V TGCA 4 cut(s) 383, 593, 650, 686
HpyF10VI GCNNNNNNNGC 4 cut(s) 20, 43, 62, 590
HpyF3I CTNAG 7 cut(s) 19, 52, 110, 166, 228, 369, 735
HpySE526I ACGT 1 cut(s) 781
Hsp92I GRCGYC 2 cut(s) 212, 781
Hsp92II CATG 4 cut(s) 256, 514, 634, 743
HspAI GCGC 2 cut(s) 44, 46
Kpn2I TCCGGA 1 cut(s) 298
KroI GCCGGC 1 cut(s) 149
KroNI GCCGGC 1 cut(s) 151
LmnI GCTCC 3 cut(s) 61, 192, 286
Lsp1109I GCAGC 3 cut(s) 168, 571, 652
LweI GCATC 3 cut(s) 90, 317, 831
MaeII ACGT 1 cut(s) 781
MaeIII GTNAC 3 cut(s) 247, 364, 612
MboII GAAGA 1 cut(s) 712
MfeI CAATTG 1 cut(s) 645
MhlI GDGCHC 1 cut(s) 67
MluCI AATT 5 cut(s) 330, 439, 470, 645, 762
MmeI TCCRAC 2 cut(s) 153, 724
MreI CGCCGGCG 1 cut(s) 149
MroI TCCGGA 1 cut(s) 298
MroNI GCCGGC 1 cut(s) 149
MroXI GAANNNNTTC 1 cut(s) 604
MspI CCGG 4 cut(s) 150, 177, 209, 299
MspR9I CCNGG 1 cut(s) 305
MunI CAATTG 1 cut(s) 645
MvaI CCWGG 1 cut(s) 305
MvnI CGCG 3 cut(s) 44, 46, 240
MwoI GCNNNNNNNGC 4 cut(s) 20, 43, 62, 590
NaeI GCCGGC 1 cut(s) 151
NcoI CCATGG 1 cut(s) 252
NgoMIV GCCGGC 1 cut(s) 149
NlaIII CATG 4 cut(s) 256, 514, 634, 743
NlaIV GGNNCC 1 cut(s) 656
NmuCI GTSAC 1 cut(s) 247
NspI RCATGY 1 cut(s) 743
PaeR7I CTCGAG 1 cut(s) 60
PagI TCATGA 1 cut(s) 630
PauI GCGCGC 1 cut(s) 44
PdiI GCCGGC 1 cut(s) 151
PdmI GAANNNNTTC 1 cut(s) 604
PfeI GAWTC 1 cut(s) 376
PkrI GCNGC 5 cut(s) 155, 158, 571, 586, 642
Psp6I CCWGG 1 cut(s) 303
PspFI CCCAGC 1 cut(s) 800
PspGI CCWGG 1 cut(s) 303
PspN4I GGNNCC 1 cut(s) 656
PspPI GGNCC 1 cut(s) 301
PspXI VCTCGAGB 1 cut(s) 60
PstNI CAGNNNCTG 1 cut(s) 368
PteI GCGCGC 1 cut(s) 44
RsaI GTAC 4 cut(s) 404, 434, 479, 837
RsaNI GTAC 4 cut(s) 403, 433, 478, 836
SatI GCNGC 5 cut(s) 154, 157, 570, 585, 641
Sau96I GGNCC 1 cut(s) 301
ScrFI CCNGG 1 cut(s) 305
SduI GDGCHC 1 cut(s) 67
SfaNI GCATC 3 cut(s) 90, 317, 831
Sfr274I CTCGAG 1 cut(s) 60
SgrAI CRCCGGYG 1 cut(s) 149
SinI GGWCC 1 cut(s) 301
SlaI CTCGAG 1 cut(s) 60
SmlI CTYRAG 1 cut(s) 60
SmoI CTYRAG 1 cut(s) 60
Sse9I AATT 5 cut(s) 330, 439, 470, 645, 762
SsiI CCGC 8 cut(s) 12, 79, 147, 153, 190, 240, 514, 570
SspI AATATT 1 cut(s) 791
StyD4I CCNGG 1 cut(s) 303
StyI CCWWGG 3 cut(s) 252, 456, 658
TaaI ACNGT 1 cut(s) 605
TaiI ACGT 1 cut(s) 784
TaqI TCGA 2 cut(s) 61, 752
TaqII GACCGA 1 cut(s) 770
TasI AATT 5 cut(s) 330, 439, 470, 645, 762
TatI WGTACW 1 cut(s) 432
TauI GCSGC 2 cut(s) 156, 572
TfiI GAWTC 1 cut(s) 376
TscAI CASTG 1 cut(s) 451
TseFI GTSAC 1 cut(s) 247
TseI GCWGC 3 cut(s) 156, 584, 640
Tsp45I GTSAC 1 cut(s) 247
TspDTI ATGAA 1 cut(s) 861
TspRI CASTG 1 cut(s) 451
VpaK11BI GGWCC 1 cut(s) 301
XagI CCTNNNNNAGG 1 cut(s) 887
XapI RAATTY 1 cut(s) 439
XceI RCATGY 1 cut(s) 743
XhoI CTCGAG 1 cut(s) 60
XmnI GAANNNNTTC 1 cut(s) 604
ZraI GACGTC 1 cut(s) 782
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.